Cp4.1LG02g00730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g00730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionindole-3-acetate O-methyltransferase 1-like
LocationCp4.1LG02: 5049608 .. 5052554 (-)
RNA-Seq ExpressionCp4.1LG02g00730
SyntenyCp4.1LG02g00730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAATAAAATATTAAATTACTCCCTAATCCTATTGAGTTTACGGTTAATAACAAAATCCGGCGGAAATCTAGGACTTTTTTCTTTTATGCATGTGNCCCTTTTTTTTTTTTTTTTAATTTTTTTTTATTACTATAATTTATTTATTTTTTTTAGCCCTTTTGTTCTTTATAAATATCCAACTCTCTTAAGCCAACCACTTACTCATAACTCAAGCCCCCCTTCCCTCCAAGCTTAGAGATCAAGCTTAAGCCCTTCCCATGGCTCCCAAGGGCGATAACGTGGTCGTTTCGAACATGAAACTCGAGCGAATGTTGAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTATGCCGACAATTCTCAAGCTCAGGTACAGACCTTTTTTTAGCTCGAGTTTAGTTACTAGTTAAGTTTGTTTTCATCAAAACATATAAACGTTCGTTCTCAATTATTATAACTCTATATTAGCGATTCTCAACTGGGTTTGTAATATAAAATTAATAACCCGACTTAATTTGACTGTTCTGGGTTTTTAGCTCGAGTTGAGTTACTAAAAATATATGGTCGTTATAATTTGCACATGGGTTCGTAATATAAAATTAATAATCCAACTTAATTTGGTTGTTCTGGGTTTTTAGCTCGAGTTGAGTTACTGAAAACATATGGTCGTTATAATTTGCATCTGGGTTTGTAACAGGCTCAACATGCTAGATCGATGCTTCATCTTCTTAAGGAAACATTGGACGGAGTTCATTTGAGCTCGATGGATGTTCCGTTCGTGGTGGTCGATCTCGGCTGCAGCTGTGGTAGTAACACCATTTACATTGTGGACGTAATCATCAAGCATATTATAAAGCGGTTCGAGGCTTTGGCCGTCGACCCGCCGGAATTCACAGCGTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACCCTCTTCCAGCTACTCCCGCCGTTAGCCTCCTACGGCAGCAGCATGGAGGAGTGCCTGGCAGCTGACAACCACCGTTCCTACTTTGCCGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCAAGGTCCATCGACGTGTTTCACTCCGCCTTTTCGTTGCATTGGCTCTCTCAGGTTTTTCATAATATTTGCGTGCTTTTTAAGTTCCATAATTTTCGTTTAAATATTATTATTATTATTATTTATTTTTGGTATTTGCATGGCATTAAAAAATTGAAATTTTTTATTTTTTTATTTTTGGTGAAATTTAATTTTTATTCAAAATTTTTCAAATTTTTAATTATTTAATAAAAAAAAATAAAATCAGCTTTTAACCCAAAAGGAAAAAAAAAACATTTAAAACTATATAATAAATATTTAGAAATTAAAATCGAATATATTTTAATTTGATTGGAACATGTGGGTCCCACCGACACCACAGGTACCGGAGAGCGTGGTAGATGCGAGATCGACGGCGTACAACAGAGGGAGGGTGTTCATCCACGGCGCAAATGAGGCGGCGGCGGCCGCATACCGGAAGCAGTTTCAGACGGACCTTGCCGGATTCCTCCAAGCCAGGGCCCAGGAGCTGAAGAGGAGTGGGTCCATGTTCCTTGTCTGCTTGGGTCGTACCTCACTGGACCCCACCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAAGATGCTTGGGATGATCTCGTCCAAGAGGTACCAAATTGCAATTATTGAAATTATATGGATGGAATTTAATATATATCTAAATTATAAGGACTAAATTAAAATTTGTGGTAGGGGCCAAAAAGAAATCCCGACAAAATAATCACAGCCGTTGATATTTTTGTTTCATACGGAACTATCTTACGTAAAAATGCAGCTGAATAATTCCCCCACCACCTGTTTTATATCTCTCCTTTACCAGACCAAAAAGCAAAAATCACTTTATTTCAAGAGATAATGATTCAGTGCTGCCGGTCTCACTGAACCCCTTTTTTTTTAATACTTTTATGATGAACATCTATCGGTCATGATTAAACCCCATCATTTCTACTCGTCTGATGTCCCCTCCGACAACTCCATCCATCCGTCCACTTGTCGGAAGGAGAATGATTGTGTTCCCCGACAATATTGTGTTATTATTGTTTCATGGAATTGCATGCATTATCCACATTTTGCTCTTTTTAATTTAAATATAATTTAATTAATTTTGGTATTCAGGGACTAATAAGCAACGAAAAACGTGATAGTTTCAACATTCCTGTGTACGCACCGAGCTTGCAAGACTTTAAGGAAGTAGTAGAAGCAGATGGTTCGTTTTCCATAAACAAGCTTGAAGTTTTCAAAGGAGGTAGTCCATTAGTAGTGAACCAGCCCGACGATGCTGCAGAGGTTGGTCGAGCTTTAGCCAACAGTTGTCGAAGTGTCTCTGGTGTTCTTGTTGACGCCCATATTGGTGATCGACTCGGGGATGAACTCTTCTCTCGAGTCGAACGTAGAGCTACAAATCATGCTAAAGACCTTCTCGAGAAGCTTCAATTCTTTCATATTGTGGCATCGCTTTCCCTTGCATAAAAAAAAACACAAAAATTATGGTTTCTTCACCATTTTCTCTCATTATAACTTTGTTAGTGTGTTGTTTTGAGTCATTTTACTGACTGTGTATTGTTCATCTTGGCTTCTTGGAATCCCGAAAAGAAACATTGTAAAAAGAAGCTAAGTGTGCAGTGTAACCCTTCTCTTAGTTTAAGGCTTATTTGTATGTTCATAAATCTCAAACGTCTCTCTTTGTATGCAATCGAAAGACTTTATAAAACCAAAGTAGTTGTGTATTTATGGATGTACGTTCGATTGAGATCGGTGTCGGGGGCTGACAAGTAAGGCCTGTTTCAAGTAGAGGAAGATTAGGGTTGTTTCGAGAGTATTATAACGTTCATATTATGTATTAGATTGAGATCAGTGT

mRNA sequence

AAATAATAAAATATTAAATTACTCCCTAATCCTATTGAGTTTACGGTTAATAACAAAATCCGGCGGAAATCTAGGACTTTTTTCTTTTATGCATGTGNCCCTTTTTTTTTTTTTTTTAATTTTTTTTTATTACTATAATTTATTTATTTTTTTTAGCCCTTTTGTTCTTTATAAATATCCAACTCTCTTAAGCCAACCACTTACTCATAACTCAAGCCCCCCTTCCCTCCAAGCTTAGAGATCAAGCTTAAGCCCTTCCCATGGCTCCCAAGGGCGATAACGTGGTCGTTTCGAACATGAAACTCGAGCGAATGTTGAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTATGCCGACAATTCTCAAGCTCAGGCTCAACATGCTAGATCGATGCTTCATCTTCTTAAGGAAACATTGGACGGAGTTCATTTGAGCTCGATGGATGTTCCGTTCGTGGTGGTCGATCTCGGCTGCAGCTGTGGTAGTAACACCATTTACATTGTGGACGTAATCATCAAGCATATTATAAAGCGGTTCGAGGCTTTGGCCGTCGACCCGCCGGAATTCACAGCGTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACCCTCTTCCAGCTACTCCCGCCGTTAGCCTCCTACGGCAGCAGCATGGAGGAGTGCCTGGCAGCTGACAACCACCGTTCCTACTTTGCCGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCAAGGTCCATCGACGTGTTTCACTCCGCCTTTTCGTTGCATTGGCTCTCTCAGGTACCGGAGAGCGTGGTAGATGCGAGATCGACGGCGTACAACAGAGGGAGGGTGTTCATCCACGGCGCAAATGAGGCGGCGGCGGCCGCATACCGGAAGCAGTTTCAGACGGACCTTGCCGGATTCCTCCAAGCCAGGGCCCAGGAGCTGAAGAGGAGTGGGTCCATGTTCCTTGTCTGCTTGGGTCGTACCTCACTGGACCCCACCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAAGATGCTTGGGATGATCTCGTCCAAGAGGGACTAATAAGCAACGAAAAACGTGATAGTTTCAACATTCCTGTGTACGCACCGAGCTTGCAAGACTTTAAGGAAGTAGTAGAAGCAGATGGTTCGTTTTCCATAAACAAGCTTGAAGTTTTCAAAGGAGGTAGTCCATTAGTAGTGAACCAGCCCGACGATGCTGCAGAGGTTGGTCGAGCTTTAGCCAACAGTTGTCGAAGTGTCTCTGGTGTTCTTGTTGACGCCCATATTGGTGATCGACTCGGGGATGAACTCTTCTCTCGAGTCGAACGTAGAGCTACAAATCATGCTAAAGACCTTCTCGAGAAGCTTCAATTCTTTCATATTGTGGCATCGCTTTCCCTTGCATAAAAAAAAACACAAAAATTATGGTTTCTTCACCATTTTCTCTCATTATAACTTTGTTAGTGTGTTGTTTTGAGTCATTTTACTGACTGTGTATTGTTCATCTTGGCTTCTTGGAATCCCGAAAAGAAACATTGTAAAAAGAAGCTAAGTGTGCAGTGTAACCCTTCTCTTAGTTTAAGGCTTATTTGTATGTTCATAAATCTCAAACGTCTCTCTTTGTATGCAATCGAAAGACTTTATAAAACCAAAGTAGTTGTGTATTTATGGATGTACGTTCGATTGAGATCGGTGTCGGGGGCTGACAAGTAAGGCCTGTTTCAAGTAGAGGAAGATTAGGGTTGTTTCGAGAGTATTATAACGTTCATATTATGTATTAGATTGAGATCAGTGT

Coding sequence (CDS)

ATGGCTCCCAAGGGCGATAACGTGGTCGTTTCGAACATGAAACTCGAGCGAATGTTGAGCATGAAAGGCGGCAAAGGCGAAGGAAGCTATGCCGACAATTCTCAAGCTCAGGCTCAACATGCTAGATCGATGCTTCATCTTCTTAAGGAAACATTGGACGGAGTTCATTTGAGCTCGATGGATGTTCCGTTCGTGGTGGTCGATCTCGGCTGCAGCTGTGGTAGTAACACCATTTACATTGTGGACGTAATCATCAAGCATATTATAAAGCGGTTCGAGGCTTTGGCCGTCGACCCGCCGGAATTCACAGCGTTTTTCTCCGATCTCCCTGGAAATGACTTCAATACCCTCTTCCAGCTACTCCCGCCGTTAGCCTCCTACGGCAGCAGCATGGAGGAGTGCCTGGCAGCTGACAACCACCGTTCCTACTTTGCCGCCGGCGTTCCTGGCTCCTTCTACCGCCGTCTGTTTCCGGCAAGGTCCATCGACGTGTTTCACTCCGCCTTTTCGTTGCATTGGCTCTCTCAGGTACCGGAGAGCGTGGTAGATGCGAGATCGACGGCGTACAACAGAGGGAGGGTGTTCATCCACGGCGCAAATGAGGCGGCGGCGGCCGCATACCGGAAGCAGTTTCAGACGGACCTTGCCGGATTCCTCCAAGCCAGGGCCCAGGAGCTGAAGAGGAGTGGGTCCATGTTCCTTGTCTGCTTGGGTCGTACCTCACTGGACCCCACCGACCAAGGTGGGGCCGGCCTCCTCTTTGGGACCCACTTTCAAGATGCTTGGGATGATCTCGTCCAAGAGGGACTAATAAGCAACGAAAAACGTGATAGTTTCAACATTCCTGTGTACGCACCGAGCTTGCAAGACTTTAAGGAAGTAGTAGAAGCAGATGGTTCGTTTTCCATAAACAAGCTTGAAGTTTTCAAAGGAGGTAGTCCATTAGTAGTGAACCAGCCCGACGATGCTGCAGAGGTTGGTCGAGCTTTAGCCAACAGTTGTCGAAGTGTCTCTGGTGTTCTTGTTGACGCCCATATTGGTGATCGACTCGGGGATGAACTCTTCTCTCGAGTCGAACGTAGAGCTACAAATCATGCTAAAGACCTTCTCGAGAAGCTTCAATTCTTTCATATTGTGGCATCGCTTTCCCTTGCATAA

Protein sequence

MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVERRATNHAKDLLEKLQFFHIVASLSLA
Homology
BLAST of Cp4.1LG02g00730 vs. ExPASy Swiss-Prot
Match: Q9FLN8 (Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 5.4e-172
Identity = 297/385 (77.14%), Postives = 337/385 (87.53%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHL--S 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYA+NSQAQA HARSMLHLL+ETL+ VHL  S
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  SMDVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLF 120
           +   PF  VDLGCS G+NT++I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVP 180
           QLLPPL S  + MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID FHSAFSLHWLSQVP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ESVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLG 240
           ESV D RS AYNRGRVFIHGA E    AY++QFQ DLA FL+ARA E+KR G+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEAD 300
           RTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD FNIPVYAPSLQDFKEVV+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ L ++LFSRV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 384
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Cp4.1LG02g00730 vs. ExPASy Swiss-Prot
Match: Q0J998 (Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 7.8e-147
Identity = 262/395 (66.33%), Postives = 313/395 (79.24%), Query Frame = 0

Query: 4   KGDNVVVS------NMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDG-VH 63
           KG+NV VS        KL  ML MKGG G+GSY +NSQAQA HAR MLH L+ETLD  + 
Sbjct: 9   KGENVTVSAAAAPRMKKLASMLCMKGGNGDGSYLNNSQAQALHARRMLHFLEETLDAMME 68

Query: 64  LSSMDVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNT 123
            SS D  F   DLGCSCGSN+++IVDVI++ + + +E+   D PEF  FFSDLP NDFNT
Sbjct: 69  RSSSDKLFTAADLGCSCGSNSLFIVDVIVRRVSEAYESRGRDAPEFQVFFSDLPSNDFNT 128

Query: 124 LFQLLPP-LASYGSSMEECLAADN-----HRSYFAAGVPGSFYRRLFPARSIDVFHSAFS 183
           LFQLLPP LA    S+EECLAA        R Y AAGVPG+FY RLFP  SIDVF S FS
Sbjct: 129 LFQLLPPLLAPVAGSLEECLAAGEGAATATRPYHAAGVPGTFYGRLFPGESIDVFTSTFS 188

Query: 184 LHWLSQVPESVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSG 243
           LHWLSQVPE V D+ S AYN GRVF+H A EA AAAY++QFQ DLA FL++RA+E+KR G
Sbjct: 189 LHWLSQVPEEVGDSASPAYNGGRVFVHRATEAVAAAYKRQFQADLARFLRSRAREMKRGG 248

Query: 244 SMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQD 303
           +MFL CLGR+S DP DQGGAGLLFGTHFQDAWDDLVQEG++  EKRDSFNIPVYAPSLQ+
Sbjct: 249 AMFLACLGRSSGDPADQGGAGLLFGTHFQDAWDDLVQEGVVEGEKRDSFNIPVYAPSLQE 308

Query: 304 FKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL 363
           F++VV ADG+F+I++LE+ +GGSPLVV++PDDAAEVGRA+ANSC++V+GVLVDAHIG+R 
Sbjct: 309 FRDVVRADGAFAIDRLELVRGGSPLVVDRPDDAAEVGRAMANSCKAVAGVLVDAHIGERR 368

Query: 364 GDELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 386
           G +LF R+ERRA  HA++L+EK+ FFH+V SLSLA
Sbjct: 369 GAQLFERLERRAARHARELVEKMHFFHVVCSLSLA 403

BLAST of Cp4.1LG02g00730 vs. ExPASy Swiss-Prot
Match: Q5XF78 (Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.8e-58
Identity = 138/379 (36.41%), Postives = 198/379 (52.24%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVPFVVVDLGCSCG 74
           L R+ +M+GG+ + SY +NS +QA        +L  +L  + L S   P  + DLGC+ G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTIYIVDVIIKHIIKRFEAL--AVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I V  S  +LHWLSQ+PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGG--- 254
           + +I GA +    AY +Q   DL  F+  R +E+ + G +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 374
            K     +V   D        D    GRA  N  ++    +VDA++G  L  ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

Query: 375 RATNHAKDLLEKLQFFHIV 380
           R + + ++ L    F+ +V
Sbjct: 378 RVSTN-QEFLHITCFYGVV 380

BLAST of Cp4.1LG02g00730 vs. ExPASy Swiss-Prot
Match: A0A061DFA8 (Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 PE=3 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 1.3e-56
Identity = 132/351 (37.61%), Postives = 191/351 (54.42%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVPFVVVDLGCSCG 74
           +E++LSM GG GE SYA+NS  Q+      + +LK+ +     +++     + DLGCS G
Sbjct: 1   MEQILSMNGGSGEQSYANNSIGQSTCLSRSMPVLKQAVLDSCCTNLPETVTMADLGCSSG 60

Query: 75  SNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSMEEC 134
            NT Y V  I   I KR   L    PEF  F +DLPGNDFNT+FQ LP  A  G   EE 
Sbjct: 61  PNTFYAVSEITSIIYKRCCQLGRSAPEFRVFLNDLPGNDFNTVFQSLP--AYQGKLREE- 120

Query: 135 LAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRV 194
                    + AGVPGSFY RLFP +++   HS+ SLHWLSQVP  + D  +   N+ ++
Sbjct: 121 -NGPGFGPLYVAGVPGSFYGRLFPLKTLHFVHSSSSLHWLSQVPPELTDKANPLINKWKI 180

Query: 195 FIHGAN-EAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGGAGLL 254
           FI   +  A  ++Y  QF+ D + FL+ R++E+   G M L   GRT+ DPT       L
Sbjct: 181 FISKTSPPAVISSYLTQFRKDFSLFLKLRSEEVAPEGRMVLTFRGRTTADPTSD--ESCL 240

Query: 255 FGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEVF---- 314
              +   A+ DLV EGL+  E+ D++N P Y P  ++ +  +E +GSF++++LEV     
Sbjct: 241 LWDYLGQAFQDLVAEGLVEEERLDTYNTPYYEPHPEEIQAEIEKEGSFTLDRLEVIALPW 300

Query: 315 ---KGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSR 358
               GG        DD A   + +A + R+V+  +  +H G+ + D LF R
Sbjct: 301 DSVNGGI------KDDRATTAKRMAKAIRAVNESMFRSHFGEDILDPLFHR 339

BLAST of Cp4.1LG02g00730 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 8.6e-53
Identity = 131/374 (35.03%), Postives = 204/374 (54.55%), Query Frame = 0

Query: 13  MKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVPFVVVDLGCS 72
           M++  +L M GG G+ SYA+NS  Q +       + ++ +  ++ S       + DLGCS
Sbjct: 1   MEVVEVLHMNGGNGDSSYANNSLVQQKVILMTKPITEQAMIDLYSSLFPETLCIADLGCS 60

Query: 73  CGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSME 132
            G+NT  +V  I+K + K  +      PEF   F+DLPGNDFNTLFQ      S G+  E
Sbjct: 61  LGANTFLVVSQIVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQ------SLGAFQE 120

Query: 133 ECL--AADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYN 192
           +      ++    F +GVPGSFY RLFP++S+   +S++SL WLSQVP  + +      N
Sbjct: 121 DLRKHIGESFGPCFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIEN------N 180

Query: 193 RGRVFIHGANE-AAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGG 252
           +G +++   +  +   AY KQ++ D + FL+ R++EL + G M L  LGR S DPT +  
Sbjct: 181 KGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSK-- 240

Query: 253 AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEVF 312
                      A ++LV+EGLI  EK D+FNIP Y PS  + K +VE +GSF+IN+LE  
Sbjct: 241 ECCYIWELLAMALNELVKEGLIKEEKVDAFNIPQYTPSPAEVKYLVEKEGSFTINRLET- 300

Query: 313 KGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVERRATNHAKDL 372
              S +  N  ++    G  ++   R+V+  L+ +H    L D +F + E   ++     
Sbjct: 301 ---SRVHWNASNNVKNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIISDCMSK- 354

Query: 373 LEKLQFFHIVASLS 384
            EK +F +++ SL+
Sbjct: 361 -EKTEFINVIVSLT 354

BLAST of Cp4.1LG02g00730 vs. NCBI nr
Match: XP_023525362.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758 bits (1958), Expect = 3.64e-276
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. NCBI nr
Match: XP_022940235.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata] >KAG7037358.1 Indole-3-acetate O-methyltransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 757 bits (1954), Expect = 1.48e-275
Identity = 384/385 (99.74%), Postives = 384/385 (99.74%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGR LANSCRSVSGVLVDAHIGDRLGDELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRVLANSCRSVSGVLVDAHIGDRLGDELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. NCBI nr
Match: XP_022981508.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 747 bits (1929), Expect = 9.59e-272
Identity = 380/385 (98.70%), Postives = 381/385 (98.96%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAP GDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM
Sbjct: 1   MAPMGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALA+DPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAIDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVE DGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEEDGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLG ELFSRVE 
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGVELFSRVEC 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. NCBI nr
Match: XP_038897278.1 (indole-3-acetate O-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 726 bits (1873), Expect = 3.29e-263
Identity = 367/385 (95.32%), Postives = 375/385 (97.40%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSNMKLERMLSMKGGKGE SYA+NSQAQAQHARSMLHLLKETLDGVHL+S 
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           +VPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVD RSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFL ARA ELKR GSMFLVCLGRT
Sbjct: 181 VVDTRSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           F INKLEVFKGGSPLVVNQPD+AAEVGRALANSCRS+SGVLVDAHIGDRL +ELF RVER
Sbjct: 301 FYINKLEVFKGGSPLVVNQPDNAAEVGRALANSCRSISGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. NCBI nr
Match: XP_022936266.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 723 bits (1867), Expect = 2.70e-262
Identity = 365/385 (94.81%), Postives = 376/385 (97.66%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYA+NSQAQAQHARS+LHLLKETLDGVHL+S 
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           ++PFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVD RSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFL ARA ELKR GSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLIS+EKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL +ELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. ExPASy TrEMBL
Match: A0A6J1FQ01 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445917 PE=4 SV=1)

HSP 1 Score: 757 bits (1954), Expect = 7.18e-276
Identity = 384/385 (99.74%), Postives = 384/385 (99.74%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM
Sbjct: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGR LANSCRSVSGVLVDAHIGDRLGDELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRVLANSCRSVSGVLVDAHIGDRLGDELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. ExPASy TrEMBL
Match: A0A6J1J2A7 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480601 PE=4 SV=1)

HSP 1 Score: 747 bits (1929), Expect = 4.64e-272
Identity = 380/385 (98.70%), Postives = 381/385 (98.96%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAP GDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM
Sbjct: 1   MAPMGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALA+DPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAIDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT
Sbjct: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVE DGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEEDGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLG ELFSRVE 
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGVELFSRVEC 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. ExPASy TrEMBL
Match: A0A5D3DHL8 (Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00180 PE=4 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.31e-262
Identity = 367/385 (95.32%), Postives = 373/385 (96.88%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           M PKGDNVVVSNMKLERMLSMKGGKGE SYA+NSQAQAQHARSMLHLLKETLDGVHL+S 
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           +VPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLA FL ARAQELKR GSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAEFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL +ELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. ExPASy TrEMBL
Match: A0A6J1F711 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442937 PE=4 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.31e-262
Identity = 365/385 (94.81%), Postives = 376/385 (97.66%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           MAPKGDNVVVSN+KLERMLSMKGGKGE SYA+NSQAQAQHARS+LHLLKETLDGVHL+S 
Sbjct: 1   MAPKGDNVVVSNIKLERMLSMKGGKGEASYANNSQAQAQHARSVLHLLKETLDGVHLNSP 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           ++PFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEF+AFFSDLPGNDFNTLFQL
Sbjct: 61  ELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFSAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVD RSTAYNRGRV IHGANEAAAAAYRKQFQTDLAGFL ARA ELKR GSMFLVCLGRT
Sbjct: 181 VVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAGFLWARAHELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLIS+EKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL +ELFSRVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFSRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. ExPASy TrEMBL
Match: A0A0A0K2W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1)

HSP 1 Score: 723 bits (1865), Expect = 2.64e-262
Identity = 365/385 (94.81%), Postives = 373/385 (96.88%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSM 60
           M PKGDNVVVSNMKLERMLSMKGGKGE SYA+NSQAQAQHARSMLHLLKETLDGVHL+S 
Sbjct: 1   MTPKGDNVVVSNMKLERMLSMKGGKGETSYANNSQAQAQHARSMLHLLKETLDGVHLNSP 60

Query: 61  DVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120
           + PFVVVDLGCSCGSNTIYI+DVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL
Sbjct: 61  EEPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQL 120

Query: 121 LPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPES 180
           LPPLA+YG SMEECLAADNHRSYFAAGVPGSFYRRLFPARSID+FHSAFSLHWLSQVPE+
Sbjct: 121 LPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDLFHSAFSLHWLSQVPET 180

Query: 181 VVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRT 240
           VVD RS AYNRGRVFIHGANEAAA AYRKQFQTDLAGFL ARAQELKR GSMFLVCLGRT
Sbjct: 181 VVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAGFLWARAQELKRGGSMFLVCLGRT 240

Query: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300
           SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS
Sbjct: 241 SLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGS 300

Query: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 360
           FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRL +ELF RVER
Sbjct: 301 FSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLSEELFYRVER 360

Query: 361 RATNHAKDLLEKLQFFHIVASLSLA 385
           RATNHAKDLLEKLQFFHIVASLSLA
Sbjct: 361 RATNHAKDLLEKLQFFHIVASLSLA 385

BLAST of Cp4.1LG02g00730 vs. TAIR 10
Match: AT5G55250.1 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 605.1 bits (1559), Expect = 3.8e-173
Identity = 297/385 (77.14%), Postives = 337/385 (87.53%), Query Frame = 0

Query: 1   MAPKGDNVVVSNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHL--S 60
           M  KGDNV V NMKLER+LSMKGGKG+ SYA+NSQAQA HARSMLHLL+ETL+ VHL  S
Sbjct: 1   MGSKGDNVAVCNMKLERLLSMKGGKGQDSYANNSQAQAMHARSMLHLLEETLENVHLNSS 60

Query: 61  SMDVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLF 120
           +   PF  VDLGCS G+NT++I+D I+KHI KRF+A  +DPPEFTAFFSDLP NDFNTLF
Sbjct: 61  ASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGIDPPEFTAFFSDLPSNDFNTLF 120

Query: 121 QLLPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVP 180
           QLLPPL S  + MEECLAAD +RSYF AGVPGSFYRRLFPAR+ID FHSAFSLHWLSQVP
Sbjct: 121 QLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLFPARTIDFFHSAFSLHWLSQVP 180

Query: 181 ESVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLG 240
           ESV D RS AYNRGRVFIHGA E    AY++QFQ DLA FL+ARA E+KR G+MFLVCLG
Sbjct: 181 ESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEFLRARAAEVKRGGAMFLVCLG 240

Query: 241 RTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEAD 300
           RTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD FNIPVYAPSLQDFKEVV+A+
Sbjct: 241 RTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDGFNIPVYAPSLQDFKEVVDAN 300

Query: 301 GSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRV 360
           GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV+GVLV+AHIG+ L ++LFSRV
Sbjct: 301 GSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVAGVLVEAHIGEELSNKLFSRV 360

Query: 361 ERRATNHAKDLLEKLQFFHIVASLS 384
           E RAT+HAKD+L  LQFFHIVASLS
Sbjct: 361 ESRATSHAKDVLVNLQFFHIVASLS 384

BLAST of Cp4.1LG02g00730 vs. TAIR 10
Match: AT5G55250.2 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 547.7 bits (1410), Expect = 7.2e-156
Identity = 267/346 (77.17%), Postives = 304/346 (87.86%), Query Frame = 0

Query: 40  HARSMLHLLKETLDGVHL--SSMDVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAV 99
           HARSMLHLL+ETL+ VHL  S+   PF  VDLGCS G+NT++I+D I+KHI KRF+A  +
Sbjct: 2   HARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGI 61

Query: 100 DPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSMEECLAADNHRSYFAAGVPGSFYRRLF 159
           DPPEFTAFFSDLP NDFNTLFQLLPPL S  + MEECLAAD +RSYF AGVPGSFYRRLF
Sbjct: 62  DPPEFTAFFSDLPSNDFNTLFQLLPPLVS-NTCMEECLAADGNRSYFVAGVPGSFYRRLF 121

Query: 160 PARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVFIHGANEAAAAAYRKQFQTDLAG 219
           PAR+ID FHSAFSLHWLSQVPESV D RS AYNRGRVFIHGA E    AY++QFQ DLA 
Sbjct: 122 PARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAE 181

Query: 220 FLQARAQELKRSGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRD 279
           FL+ARA E+KR G+MFLVCLGRTS+DPTDQGGAGLLFGTHFQDAWDDLV+EGL++ EKRD
Sbjct: 182 FLRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRD 241

Query: 280 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 339
            FNIPVYAPSLQDFKEVV+A+GSF+I+KL V+KGGSPLVVN+PDDA+EVGRA A+SCRSV
Sbjct: 242 GFNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSV 301

Query: 340 SGVLVDAHIGDRLGDELFSRVERRATNHAKDLLEKLQFFHIVASLS 384
           +GVLV+AHIG+ L ++LFSRVE RAT+HAKD+L  LQFFHIVASLS
Sbjct: 302 AGVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 346

BLAST of Cp4.1LG02g00730 vs. TAIR 10
Match: AT5G56300.1 (gibberellic acid methyltransferase 2 )

HSP 1 Score: 228.0 bits (580), Expect = 1.3e-59
Identity = 138/379 (36.41%), Postives = 198/379 (52.24%), Query Frame = 0

Query: 15  LERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVPFVVVDLGCSCG 74
           L R+ +M+GG+ + SY +NS +QA        +L  +L  + L S   P  + DLGC+ G
Sbjct: 18  LHRVFAMQGGEDDLSYVNNSDSQALAITLSKPILISSLQSIKLFSDQTPIKITDLGCATG 77

Query: 75  SNTIYIVDVIIKHIIKRFEAL--AVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSME 134
           SNT   VD +++ + +R+ A       PEF AFF DLP NDFN LF+LL           
Sbjct: 78  SNTFTTVDTVVETLQRRYTARCGGGGSPEFEAFFCDLPSNDFNMLFKLL----------A 137

Query: 135 ECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRG 194
           E    D+   YFA GV GSFY RLFP  +I V  S  +LHWLSQ+PE V++  S  +N+G
Sbjct: 138 EKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWLSQIPEKVLEKESRTWNKG 197

Query: 195 RVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGG--- 254
           + +I GA +    AY +Q   DL  F+  R +E+ + G +F++  GR S   +  G    
Sbjct: 198 KTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFVLMAGRPSGSSSQFGDQDT 257

Query: 255 -AGLLFGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV 314
            A   F T  + AW DL++EGLI  E RD FNIP Y  S ++    ++  G F I K++ 
Sbjct: 258 RAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEEVTAGIDRCGGFKIGKMDF 317

Query: 315 FKGGSPLVVNQPD--------DAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVER 374
            K     +V   D        D    GRA  N  ++    +VDA++G  L  ELF R E 
Sbjct: 318 LK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMVDAYLGPDLSHELFKRYEN 377

Query: 375 RATNHAKDLLEKLQFFHIV 380
           R + + ++ L    F+ +V
Sbjct: 378 RVSTN-QEFLHITCFYGVV 380

BLAST of Cp4.1LG02g00730 vs. TAIR 10
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 203.4 bits (516), Expect = 3.4e-52
Identity = 133/375 (35.47%), Postives = 191/375 (50.93%), Query Frame = 0

Query: 18  MLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLSSMDVP--FVVVDLGCSCGS 77
           +LSMKGG GE SYA+NS+ Q + A     ++ ET+  + +   D P    V DLGCS G 
Sbjct: 3   VLSMKGGDGEHSYANNSEGQKRLASDAKPVVVETVKEM-IVKTDFPGCIKVADLGCSSGE 62

Query: 78  NTIYIVDVIIKHIIKRFEALAVDPPEFTAFFSDLPGNDFNTLFQLLPPLASYGSSMEECL 137
           NT+ ++  I+  II  ++    + PE     +DLP NDFNT F+L+P       +  + L
Sbjct: 63  NTLLVMSEIVNTIITSYQQKGKNLPEINCCLNDLPDNDFNTTFKLVP-------AFHKLL 122

Query: 138 AADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARSTAYNRGRVF 197
             D     F +GVPGSFY RLFP++S+   HS+  LHWLS+VP+ + D +   Y R    
Sbjct: 123 KMDVKGKCFISGVPGSFYSRLFPSKSLHFVHSSLCLHWLSKVPDGLEDNKKNVYLRS--- 182

Query: 198 IHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTDQGGAGLLFG 257
                     +Y  QF+ D + FL+ RA E   +G M L  +GR SLDP  +        
Sbjct: 183 --PCPPNVYKSYLTQFKNDFSLFLRLRADETVPNGRMALTFVGRKSLDPLSKD--CFQNW 242

Query: 258 THFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFSINKLEV------- 317
           +   D+  DLV EG++     DSFN+P Y P   + +EV+E++GSF I+  E        
Sbjct: 243 SSISDSLLDLVSEGIVKESDVDSFNLPFYNPDESEVREVIESEGSFKISNFETIFGLLFS 302

Query: 318 FKGGSPLVVNQPDD-----AAEVGRALANSCRSVSGVLVDAHIGDRLGDELFSRVERRAT 377
           +K G   V +  D+       EV R  A+  RS++  ++ AH GD + D LF R      
Sbjct: 303 YKTGRTEVKDDDDNLDQSCRFEVIRKRASIIRSITEPMLGAHFGDAIMDRLFERYTYHLA 362

BLAST of Cp4.1LG02g00730 vs. TAIR 10
Match: AT4G26420.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 5.7e-52
Identity = 136/384 (35.42%), Postives = 194/384 (50.52%), Query Frame = 0

Query: 11  SNMKLERMLSMKGGKGEGSYADNSQAQAQHARSMLHLLKETLDGVHLS-SMDVPFVVVDL 70
           S+  LE +LSM+GG+ + SY  N    A        +L   ++ + L+        + DL
Sbjct: 3   SSRSLEHVLSMQGGEDDASYVKNCYGPAARLALSKPMLTTAINSIKLTEGCSSHLKIADL 62

Query: 71  GCSCGSNTIYIVDVIIKHIIKRFEAL--AVDPP-EFTAFFSDLPGNDFNTLFQLLPPLAS 130
           GC+ G NT   V+ +++ + K+   +    +P  EF  FFSDL  NDFN LF+ L    +
Sbjct: 63  GCAIGDNTFSTVETVVEVLGKKLAVIDGGTEPEMEFEVFFSDLSSNDFNALFRSLDEKVN 122

Query: 131 YGSSMEECLAADNHRSYFAAGVPGSFYRRLFPARSIDVFHSAFSLHWLSQVPESVVDARS 190
            GSS          R YFAAGVPGSFY+RLFP   + V  +  +L WLSQVPE V++  S
Sbjct: 123 -GSS----------RKYFAAGVPGSFYKRLFPKGELHVVVTMSALQWLSQVPEKVMEKGS 182

Query: 191 TAYNRGRVFIHGANEAAAAAYRKQFQTDLAGFLQARAQELKRSGSMFLVCLGRTSLDPTD 250
            ++N+G V+I GA +    AY +Q   DL  FL+ R +E+   G +F++  GR S     
Sbjct: 183 KSWNKGGVWIEGAEKEVVEAYAEQADKDLVEFLKCRKEEIVVGGVLFMLMGGRPSGSVNQ 242

Query: 251 QGGAGLL----FGTHFQDAWDDLVQEGLISNEKRDSFNIPVYAPSLQDFKEVVEADGSFS 310
            G         F T    AW DLV EGLI  EKRD FNIPVY  + ++    ++  G F 
Sbjct: 243 IGDPDSSLKHPFTTLMDQAWQDLVDEGLIEEEKRDGFNIPVYFRTTEEIAAAIDRCGGFK 302

Query: 311 INKLE------VFKGGSPLVVNQPDDAAEVGRALANSCRSVSGVLVDAHIGDRLGDELFS 370
           I K E         G    ++  PD     GR  AN  ++    +V A++G  L  +LF 
Sbjct: 303 IEKTENLIIADHMNGKQEELMKDPD---SYGRDRANYAQAGLKPIVQAYLGPDLTHKLFK 362

Query: 371 RVERRATNHAKDLLEKLQFFHIVA 381
           R   RA    K++L    F+H++A
Sbjct: 363 RYAVRAAAD-KEILNNC-FYHMIA 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLN85.4e-17277.14Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 ... [more]
Q0J9987.8e-14766.33Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5XF781.8e-5836.41Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE... [more]
A0A061DFA81.3e-5637.61Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 P... [more]
A4ZDG88.6e-5335.03S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
Match NameE-valueIdentityDescription
XP_023525362.13.64e-276100.00indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022940235.11.48e-27599.74indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata] >KAG7037358.1 I... [more]
XP_022981508.19.59e-27298.70indole-3-acetate O-methyltransferase 1-like [Cucurbita maxima][more]
XP_038897278.13.29e-26395.32indole-3-acetate O-methyltransferase 1 [Benincasa hispida][more]
XP_022936266.12.70e-26294.81indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1FQ017.18e-27699.74indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1J2A74.64e-27298.70indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5D3DHL81.31e-26295.32Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1F7111.31e-26294.81indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0K2W32.64e-26294.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55250.13.8e-17377.14IAA carboxylmethyltransferase 1 [more]
AT5G55250.27.2e-15677.17IAA carboxylmethyltransferase 1 [more]
AT5G56300.11.3e-5936.41gibberellic acid methyltransferase 2 [more]
AT5G38020.13.4e-5235.47S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G26420.15.7e-5235.42S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 18..367
e-value: 1.2E-122
score: 411.0
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 33..312
e-value: 1.2E-122
score: 411.0
NoneNo IPR availablePANTHERPTHR31009:SF42INDOLE-3-ACETATE O-METHYLTRANSFERASE 1coord: 10..384
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 61..383
e-value: 1.9E-105
score: 352.8
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 10..384
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 13..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g00730.1Cp4.1LG02g00730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity