Cp4.1LG02g00440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g00440 vs. ExPASy Swiss-Prot
Match: Q8VYM6 (26S rRNA (cytosine-C(5))-methyltransferase NOP2B OS=Arabidopsis thaliana OX=3702 GN=NOP2B PE=1 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 4.7e-194
Identity = 396/641 (61.78%), Postives = 477/641 (74.41%), Query Frame = 0

Query: 9   KKAVKRDDPQSKRK----------KKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLD 68
           KKA K   P  K++          KK E+ T +  ++EE   SD       E   D   D
Sbjct: 29  KKAAKTQKPPLKKQRKCISEKKPLKKPEVSTDEEEEEEENEQSDEGS----ESGSDLFSD 88

Query: 69  ADEQGSGSGSGSEVSSDGDD-------PLADDFFHGSDEENVSR-SVSGSDSDESDIEEK 128
            DE+G+      +   D DD       PLA+DF  GSD E V+  S   SDS  S +E K
Sbjct: 89  GDEEGNNDSDDDDDDDDDDDDDDEDAEPLAEDFLDGSDNEEVTMGSDLDSDSGGSKLERK 148

Query: 129 SRAIDEERAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVR 188
           SRAID +R +E +DA DE ++NIKE+ DEF+LPT++ELEEEA RPPD+ +LQ RIREIVR
Sbjct: 149 SRAIDRKRKKEVQDADDEFKMNIKEKPDEFQLPTQKELEEEARRPPDLPSLQMRIREIVR 208

Query: 189 VLSNFKALRQEG--ATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEK 248
           +LSNFK L+ +G    R DYV QLK D+ SYYGYNEFL G L+E+FPVVELMELIEAFEK
Sbjct: 209 ILSNFKDLKPKGDKHERNDYVGQLKADLSSYYGYNEFLIGTLIEMFPVVELMELIEAFEK 268

Query: 249 PRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFY 308
            RP  +RTNTLKTRRRDLAD+L+NRGVNLDPLSKWSKVGL+VYDSQVPIGATPEY+AGFY
Sbjct: 269 KRPTSIRTNTLKTRRRDLADILLNRGVNLDPLSKWSKVGLIVYDSQVPIGATPEYLAGFY 328

Query: 309 MLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLT 368
           MLQ ASSFLPVMALAP+EKER+VDMAAAPGGKTTY+AALMKN+GII+ANEMKVPRLKSL+
Sbjct: 329 MLQSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNTGIIYANEMKVPRLKSLS 388

Query: 369 ANLHRMGVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEI 428
           ANLHRMGVTNT+VCNYDG+EL KVLG ++ DRVLLDAPCSG+GV+SKDESVKTSKS ++I
Sbjct: 389 ANLHRMGVTNTIVCNYDGRELTKVLGQSSVDRVLLDAPCSGTGVISKDESVKTSKSADDI 448

Query: 429 QKCAQLQKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGL 488
           +K A LQKQLIL AID+VDANSK+GGYIVYSTCS+M+ ENEAV+DYALK R+VKLVPCGL
Sbjct: 449 KKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSVMIPENEAVIDYALKNRDVKLVPCGL 508

Query: 489 DFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSE---ALE 548
           DFGRPGF  FRE RFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSN +  + + E    +E
Sbjct: 509 DFGRPGFSSFREHRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNAMQPSGNDEPAVTME 568

Query: 549 EEAV---EEEDFEAE---------------KKSANEKND-GECLHPQVNDGKKRKKVVSE 608
           +  V   +++D +AE               K+ +N K D  +  +P+  +  K K+  + 
Sbjct: 569 QAQVSSSDDDDEKAEAIEELEKPPVASGQPKRESNTKEDTNKRKNPRSKEIHKGKRNKNT 628

BLAST of Cp4.1LG02g00440 vs. ExPASy Swiss-Prot
Match: Q9FG73 (25S rRNA (cytosine-C(5))-methyltransferase NOP2A OS=Arabidopsis thaliana OX=3702 GN=NOP2A PE=1 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.6e-189
Identity = 397/652 (60.89%), Postives = 474/652 (72.70%), Query Frame = 0

Query: 2   PAAKFNKKKAVKRDDPQSKRKKK---------DELFTPKRGKKEEEND----SDLSDALD 61
           P     KK A  ++ P  K++KK         D   T    + +E +D     D+S A++
Sbjct: 33  PQTSMLKKGAKSQNKPPLKKQKKEVVEEEPLEDYEVTDDSDEDDEVSDGSDEDDISPAVE 92

Query: 62  VEEVEDGMLDADEQGSGSGSGSEVSSDGDDPLADDFFHGS---DEENVSRSVSGSDSDES 121
            EE+++   D  E GS      +   + ++ L DDF  GS   DEE    + S +DSD+ 
Sbjct: 93  SEEIDES--DDGENGSNQLFSDDEEENDEETLGDDFLEGSGDEDEEGSLDADSDADSDDD 152

Query: 122 DIEEKSRAIDEERAREEKDAADEMQLNIKE--------ESDEFRLPTKEELEEEALRPPD 181
           DI  KS AID + A ++KDAA E++  IK+        E D FRLPT+EELEEEA  PPD
Sbjct: 153 DIVAKSDAIDRDLAMQKKDAAAELEDFIKQDDVHDEEPEHDAFRLPTEEELEEEARGPPD 212

Query: 182 ISNLQRRIREIVRVLSNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVV 241
           +  L+ RI EIVR L NFKA R +  TRK  VEQLK D+GSYYGYN FL G LVE+FP  
Sbjct: 213 LPLLKTRIEEIVRALKNFKAFRPKDTTRKACVEQLKADLGSYYGYNSFLIGTLVEMFPPG 272

Query: 242 ELMELIEAFEKPRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPI 301
           ELMELIEAFEK RP  +RTNTLKTRRRDLADVL+NRGVNLDPLSKWSKVGLV+YDSQVPI
Sbjct: 273 ELMELIEAFEKQRPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPI 332

Query: 302 GATPEYMAGFYMLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFAN 361
           GATPEY+AG+YMLQGASSFLPVMALAP+E ERIVD+AAAPGGKTTYIAALMKN+G+I+AN
Sbjct: 333 GATPEYLAGYYMLQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYAN 392

Query: 362 EMKVPRLKSLTANLHRMGVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDE 421
           EMKVPRLKSLTANLHRMGVTNT+VCNYDG+ELPKVLG NT DRVLLDAPCSG+G++SKDE
Sbjct: 393 EMKVPRLKSLTANLHRMGVTNTIVCNYDGRELPKVLGQNTVDRVLLDAPCSGTGIISKDE 452

Query: 422 SVKTSKSLEEIQKCAQLQKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALK 481
           SVK +K+++EI+K A LQKQL+LAAIDMVDANSK+GGYIVYSTCSIMV ENEAV+DYALK
Sbjct: 453 SVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYALK 512

Query: 482 KRNVKLVPCGLDFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT 541
           KR+VKLV CGLDFGR GF RFRE RF PSL+KTRRFYPHVHNMDGFFVAKLKKMSN    
Sbjct: 513 KRDVKLVTCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSN---V 572

Query: 542 AQSSEALEEEAVE---------EEDFEAEKKSANEKNDGECLHPQVNDGKKRKKVVSEKH 601
            QSSE  +++AVE         ++D EAE     EK       P+    KK K+ +++  
Sbjct: 573 KQSSEEGDDDAVETVEQAEVSSDDDDEAEAIEETEKPSVPVRQPKERKEKKNKEKLAKSK 632

Query: 602 EVENG------------EESKPGVEKRKKRKFPSREEISKAREEKREALREK 609
           E + G            E SKP  +K+K+R++  + EI++AREEKR A+REK
Sbjct: 633 EDKRGKKDKKSKSENVEEPSKPRKQKKKRREW--KNEIAQAREEKRIAMREK 677

BLAST of Cp4.1LG02g00440 vs. ExPASy Swiss-Prot
Match: Q922K7 (Probable 28S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nop2 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 6.6e-140
Identity = 278/524 (53.05%), Postives = 361/524 (68.89%), Query Frame = 0

Query: 32  RGKKE----EENDSDLSDALDVEEVEDGMLDADEQGSGSGSGSEVSSDGDDPLADDFFHG 91
           RGKK     + +D D       EE + G  D   QG   G     S D D+ + DD+   
Sbjct: 86  RGKKRPAPIQNSDGD-------EEEDSGEDDVVTQGDLWG-----SEDSDEDMVDDYGAA 145

Query: 92  SDEE-------------------NVSRSVSGSDSDESDIEEKSRAIDEERAREEKDAADE 151
           S+ E                   + +  V  ++ D  + E+      E   R++  A  +
Sbjct: 146 SNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGD 205

Query: 152 MQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQEGATRKDY 211
           +Q+N+++E + F LP   E +++  + PD+  + +RI++IV VL +F A R+EG +R +Y
Sbjct: 206 LQINVEDE-EAFVLPPAGETDQDG-QAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEY 265

Query: 212 VEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLKTRRRDLAD 271
           + +L+KD+ +YY Y +FL   L+ELFP+ EL+E +EA E PRPI LRTNTLKTRRRDLA 
Sbjct: 266 LSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEVPRPITLRTNTLKTRRRDLAQ 325

Query: 272 VLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVMALAPQEKE 331
           +LINRGVNLDPL KWSK GLVVYDS VPIGATPEY+AG YMLQGASS LPVMALAPQE E
Sbjct: 326 LLINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHE 385

Query: 332 RIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTVVCNYDGKE 391
           RI+DM  APGGKT+YIA LMKN+G+I AN+    RLKS+  NLHR+GVTNT++ +YDG++
Sbjct: 386 RILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVTNTIISHYDGRQ 445

Query: 392 LPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLILAAIDMVDA 451
            PKV+G    DRVLLDAPCSG+GV+SKD +VKT+K  ++IQ+CA LQK+L+L+AID V+A
Sbjct: 446 FPKVVG--GFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNA 505

Query: 452 NSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFREKRFHPSLE 511
            SK+GGY+VY TCSI V+ENE VVDYALKKRNV+LVP GLDFG+ GF RF+ +RFHP+L 
Sbjct: 506 ASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLR 565

Query: 512 KTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEE 533
            TRRFYPH HNMDGFF+AK KK SN IP   +  +      E +
Sbjct: 566 STRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPD 593

BLAST of Cp4.1LG02g00440 vs. ExPASy Swiss-Prot
Match: P46087 (Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NOP2 PE=1 SV=2)

HSP 1 Score: 496.1 bits (1276), Expect = 5.6e-139
Identity = 295/618 (47.73%), Postives = 395/618 (63.92%), Query Frame = 0

Query: 21  RKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDADEQGSGSGSGSEVSSDGDDPL 80
           +K    LF   RGKK     SD  +  +  E EDGM++  +          V   G D  
Sbjct: 91  KKGPQSLFNAPRGKKRPAPGSDEEEEEEDSE-EDGMVNHGDLWGSEDDADTVDDYGADSN 150

Query: 81  ADDFFHGSDEENVSRS------------VSGSDSDESDIEEKSRAIDEERAREEKDAADE 140
           ++D   G     + R+            +  S+ +  D EE+     E    + ++A   
Sbjct: 151 SEDEEEGEALLPIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGG 210

Query: 141 MQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQEGATRKDY 200
           +Q+N+ EE   F LP   E+E++A + PD+  + +RI++IV +L +F A R+EG +R +Y
Sbjct: 211 LQINVDEE--PFVLPPAGEMEQDA-QAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEY 270

Query: 201 VEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLKTRRRDLAD 260
           + +LKKD+  YY Y +FL G L++LFP+ EL+E +EA E PRP+ LRTNTLKTRRRDLA 
Sbjct: 271 LNRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQ 330

Query: 261 VLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVMALAPQEKE 320
            LINRGVNLDPL KWSK GLVVYDS VPIGATPEY+AG YMLQGASS LPVMALAPQE E
Sbjct: 331 ALINRGVNLDPLGKWSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHE 390

Query: 321 RIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTVVCNYDGKE 380
           RI+DM  APGGKT+Y+A LMKN+G+I AN+    RLKS+  NLHR+GVTNT++ +YDG++
Sbjct: 391 RILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQ 450

Query: 381 LPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLILAAIDMVDA 440
            PKV+G    DRVLLDAPCSG+GV+SKD +VKT+K  ++I +CA LQK+L+L+AID V+A
Sbjct: 451 FPKVVG--GFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVNA 510

Query: 441 NSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFREKRFHPSLE 500
            SK+GGY+VY TCSI V+ENE VVDYALKKRNV+LVP GLDFG+ GF RFRE+RFHPSL 
Sbjct: 511 TSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPSLR 570

Query: 501 KTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKSANEKNDGE 560
            TRRFYPH HNMDGFF+AK KK SN IP +Q+  +   E     + +  +     +N  +
Sbjct: 571 STRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNS---ETATPTNVDLPQVIPKSENSSQ 630

Query: 561 CLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE-------- 616
                    K ++++  ++H  +   +   G+ K    +  +   ++K +          
Sbjct: 631 PAKKAKGAAKTKQQLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQ 690

BLAST of Cp4.1LG02g00440 vs. ExPASy Swiss-Prot
Match: O94268 (25S rRNA (cytosine-C(5))-methyltransferase nop2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nop2 PE=1 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 7.3e-139
Identity = 290/552 (52.54%), Postives = 377/552 (68.30%), Query Frame = 0

Query: 8   KKKAVKRDDPQSKRKKKDELFTPKRG---KKEEENDSDLSDALDVEEVE---DGMLDADE 67
           KK+   ++ PQ+ RK+  +L   K+      + EN+ DL DA + EE E   D   D + 
Sbjct: 30  KKRKHSKEKPQNSRKR--QLAEEKKSLFENSDSENEKDLIDADEFEEAETLSDLEHDEEP 89

Query: 68  QGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDI-----EEKSRAIDE 127
           Q        + + + +    D  F   +E  V    S    DE D+     E  SR +DE
Sbjct: 90  QTFADEFIDDEAKECEGEEEDSVFDSDEEHEVKPMFSDDSGDEEDLELANMEAMSRKLDE 149

Query: 128 ERAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALR--PPDISNLQRRIREIVRVLSN 187
           E   EEK+A +E+  NI  E+     P     + + +   P D+S +Q RI+EIVRVL++
Sbjct: 150 EAELEEKEAEEELHTNIHPEAPTVLPPIDGFTDSQPISTLPQDLSQIQLRIQEIVRVLND 209

Query: 188 FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICL 247
           FK L + G  R +YV+QL  DI +YYGY+ FL   L ELF V E +E  EA E PRP+ +
Sbjct: 210 FKNLCEPGRNRSEYVDQLLNDICAYYGYSRFLAEKLFELFSVSEAVEFFEANEMPRPVTI 269

Query: 248 RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS 307
           RTNTLKT+RR+LA  LINRGVNL+P+ KWSKVGL V++SQVPIGATPEY+AG Y+LQ AS
Sbjct: 270 RTNTLKTQRRELAQALINRGVNLEPIGKWSKVGLQVFESQVPIGATPEYLAGHYILQAAS 329

Query: 308 SFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRM 367
           SFLPVMALAPQ  ERI+DM++APGGK TY+AAL KN+GIIFAN+    R K+L+AN+HR+
Sbjct: 330 SFLPVMALAPQPNERILDMSSAPGGKVTYVAALQKNTGIIFANDSNKARTKALSANIHRL 389

Query: 368 GVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQL 427
           GV N +VCNYDG++ P  + +   DRVLLDAPCSG+GV+ KD+SVKT+KS  +    + L
Sbjct: 390 GVRNAIVCNYDGRKFPNEV-IGGFDRVLLDAPCSGTGVIYKDQSVKTNKSERDFDTLSHL 449

Query: 428 QKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKR-NVKLVPCGLDFGRP 487
           Q+QL+L+AID V+A+SK+GG+IVYSTCSI VDE+EAV+ YALKKR NVKLV  GL+FGR 
Sbjct: 450 QRQLLLSAIDSVNADSKTGGFIVYSTCSITVDEDEAVIQYALKKRPNVKLVSTGLEFGRE 509

Query: 488 GFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEED 546
           GF RFREKRFHPSL+ TRR+YPHVHN+DGFFVAKLKK+S+KIPT   ++ +++      D
Sbjct: 510 GFTRFREKRFHPSLKLTRRYYPHVHNIDGFFVAKLKKISDKIPTVNVADDMKDGT--NND 569

BLAST of Cp4.1LG02g00440 vs. NCBI nr
Match: XP_023525424.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1150 bits (2974), Expect = 0.0
Identity = 615/615 (100.00%), Postives = 615/615 (100.00%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120
           EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR
Sbjct: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120

Query: 121 EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180
           EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ
Sbjct: 121 EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180

Query: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240
           EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK
Sbjct: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240

Query: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300
           TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM
Sbjct: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300

Query: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360
           ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV
Sbjct: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360

Query: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL 420
           VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL
Sbjct: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL 420

Query: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480
           AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE
Sbjct: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480

Query: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS 540
           KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS
Sbjct: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS 540

Query: 541 ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600
           ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE
Sbjct: 541 ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600

Query: 601 KREALREKKAGGSKK 615
           KREALREKKAGGSKK
Sbjct: 601 KREALREKKAGGSKK 615

BLAST of Cp4.1LG02g00440 vs. NCBI nr
Match: KAG6607741.1 (26S rRNA (cytosine-C(5))-methyltransferase NOP2B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 602/615 (97.89%), Postives = 609/615 (99.02%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALD EEVEDGMLD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120
           EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR
Sbjct: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120

Query: 121 EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180
           EEKDAADEMQLNIKEESDEFRLP+KEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ
Sbjct: 121 EEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180

Query: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240
           EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFP+VELMELIEAFEKPRPICLRTNTLK
Sbjct: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPIVELMELIEAFEKPRPICLRTNTLK 240

Query: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300
           TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM
Sbjct: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300

Query: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360
           ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV
Sbjct: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360

Query: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL 420
           VCNYDGKELPKVLGMNTADRVLLDAPCSGSGV+SKDESVKTSKSLEEIQKCAQLQKQLIL
Sbjct: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQLIL 420

Query: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480
           AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE
Sbjct: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480

Query: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS 540
           KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTA+SSEALEEEAVEEED EAEK S
Sbjct: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTARSSEALEEEAVEEEDIEAEK-S 540

Query: 541 ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600
           +NE N+GECLHPQ NDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE
Sbjct: 541 SNETNNGECLHPQANDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600

Query: 601 KREALREKKAGGSKK 615
           KR+ALREKKAGGSKK
Sbjct: 601 KRQALREKKAGGSKK 614

BLAST of Cp4.1LG02g00440 vs. NCBI nr
Match: XP_022981419.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Cucurbita maxima])

HSP 1 Score: 1120 bits (2897), Expect = 0.0
Identity = 603/617 (97.73%), Postives = 607/617 (98.38%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDAL  EEVEDGMLD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALGEEEVEDGMLDGD 60

Query: 61  E--QGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER 120
           E   GSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER
Sbjct: 61  ELGSGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER 120

Query: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKAL 180
           AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVL+NFKAL
Sbjct: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLTNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN
Sbjct: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360

Query: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420
           TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGV+SKDESVKTSKSLEEIQKCAQLQKQL
Sbjct: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQL 420

Query: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRF 480
           ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYAL+KRNVKLVPCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALRKRNVKLVPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEK 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT QSSEALEEEAVEEED EAEK
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTTQSSEALEEEAVEEEDIEAEK 540

Query: 541 KSANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600
            SANEK DGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR
Sbjct: 541 -SANEKTDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600

Query: 601 EEKREALREKKAGGSKK 615
           EEKR+ALREKKAGGSKK
Sbjct: 601 EEKRQALREKKAGGSKK 616

BLAST of Cp4.1LG02g00440 vs. NCBI nr
Match: XP_022941253.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 1114 bits (2882), Expect = 0.0
Identity = 599/615 (97.40%), Postives = 604/615 (98.21%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALD EEVEDGMLD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120
           EQGSGS    EVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR
Sbjct: 61  EQGSGS----EVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120

Query: 121 EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180
           EEKDAADEMQLNIKEESDEFRLP+KEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ
Sbjct: 121 EEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180

Query: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240
           EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK
Sbjct: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240

Query: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300
           TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM
Sbjct: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300

Query: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360
           ALAP+EKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV
Sbjct: 301 ALAPREKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360

Query: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL 420
           VCNYDGKELPKVLGMNTADRVLLDAPCSGSGV+SKDESVKTSKSLEEIQKCAQLQKQLIL
Sbjct: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQLIL 420

Query: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480
           AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE
Sbjct: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480

Query: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS 540
           KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEED E EK S
Sbjct: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDIETEK-S 540

Query: 541 ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600
           +NE NDGECLHPQ NDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE
Sbjct: 541 SNETNDGECLHPQANDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600

Query: 601 KREALREKKAGGSKK 615
           KR+ALREKKAGGSKK
Sbjct: 601 KRQALREKKAGGSKK 610

BLAST of Cp4.1LG02g00440 vs. NCBI nr
Match: XP_038897895.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Benincasa hispida])

HSP 1 Score: 1010 bits (2611), Expect = 0.0
Identity = 549/615 (89.27%), Postives = 578/615 (93.98%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           M AAKFNKKK VK+DDP SKRK+KDELFTPKR K EE++DSDLSDALD +EV++G++D D
Sbjct: 1   MAAAKFNKKKGVKQDDPPSKRKEKDELFTPKRSK-EEDDDSDLSDALDEDEVDNGIIDGD 60

Query: 61  EQGS-GSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERA 120
           E+   GSGSGSE SSDGDDPLA DF  GSDEEN S SVS SDSDE+DIE+KSR IDE+RA
Sbjct: 61  EEDEEGSGSGSEDSSDGDDPLAHDFLQGSDEENDSGSVSSSDSDETDIEDKSRIIDEKRA 120

Query: 121 REEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALR 180
           REEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPD+SNLQRRIREIVRVLSNFKALR
Sbjct: 121 REEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDLSNLQRRIREIVRVLSNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKDIGSYYGYNEFL GVLVE+FPVVELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNT 360
           MALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRMGVTNT
Sbjct: 301 MALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360

Query: 361 VVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLI 420
           VVCNYDGKELPKVLG+NTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCA+LQKQLI
Sbjct: 361 VVCNYDGKELPKVLGVNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAELQKQLI 420

Query: 421 LAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFR 480
           LAAIDMVD NSKSGGY+VYSTCSIMVDENEAVVDYALKKR+VKLVPCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMVDCNSKSGGYVVYSTCSIMVDENEAVVDYALKKRDVKLVPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKK 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAK KKMSNKI TA+SSE+LE EAVEE++     K
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKFKKMSNKISTARSSESLE-EAVEEDNDAG--K 540

Query: 541 SANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKARE 600
           S NEKNDGECLHPQVND KKRKKV S+ H+V NG+ESKP VEKRKKRKFPSREEI++ARE
Sbjct: 541 SGNEKNDGECLHPQVNDSKKRKKV-SQNHDVGNGKESKPTVEKRKKRKFPSREEIARARE 600

Query: 601 EKREALREKKAGGSK 614
           EKR+ALREKKAG  K
Sbjct: 601 EKRQALREKKAGNKK 610

BLAST of Cp4.1LG02g00440 vs. ExPASy TrEMBL
Match: A0A6J1J1T4 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480547 PE=3 SV=1)

HSP 1 Score: 1120 bits (2897), Expect = 0.0
Identity = 603/617 (97.73%), Postives = 607/617 (98.38%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDAL  EEVEDGMLD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALGEEEVEDGMLDGD 60

Query: 61  E--QGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER 120
           E   GSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER
Sbjct: 61  ELGSGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEER 120

Query: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKAL 180
           AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVL+NFKAL
Sbjct: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLTNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN
Sbjct: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360

Query: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420
           TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGV+SKDESVKTSKSLEEIQKCAQLQKQL
Sbjct: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQL 420

Query: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRF 480
           ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYAL+KRNVKLVPCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALRKRNVKLVPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEK 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT QSSEALEEEAVEEED EAEK
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTTQSSEALEEEAVEEEDIEAEK 540

Query: 541 KSANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600
            SANEK DGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR
Sbjct: 541 -SANEKTDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600

Query: 601 EEKREALREKKAGGSKK 615
           EEKR+ALREKKAGGSKK
Sbjct: 601 EEKRQALREKKAGGSKK 616

BLAST of Cp4.1LG02g00440 vs. ExPASy TrEMBL
Match: A0A6J1FRL9 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446614 PE=3 SV=1)

HSP 1 Score: 1114 bits (2882), Expect = 0.0
Identity = 599/615 (97.40%), Postives = 604/615 (98.21%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALD EEVEDGMLD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120
           EQGSGS    EVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR
Sbjct: 61  EQGSGS----EVSSDGDDPLADDFFHGSDEENVSRSVSGSDSDESDIEEKSRAIDEERAR 120

Query: 121 EEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180
           EEKDAADEMQLNIKEESDEFRLP+KEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ
Sbjct: 121 EEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFKALRQ 180

Query: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240
           EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK
Sbjct: 181 EGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTLK 240

Query: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300
           TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM
Sbjct: 241 TRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVM 300

Query: 301 ALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360
           ALAP+EKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV
Sbjct: 301 ALAPREKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNTV 360

Query: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLIL 420
           VCNYDGKELPKVLGMNTADRVLLDAPCSGSGV+SKDESVKTSKSLEEIQKCAQLQKQLIL
Sbjct: 361 VCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQLIL 420

Query: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480
           AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE
Sbjct: 421 AAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFRE 480

Query: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKKS 540
           KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEED E EK S
Sbjct: 481 KRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDIETEK-S 540

Query: 541 ANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600
           +NE NDGECLHPQ NDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE
Sbjct: 541 SNETNDGECLHPQANDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAREE 600

Query: 601 KREALREKKAGGSKK 615
           KR+ALREKKAGGSKK
Sbjct: 601 KRQALREKKAGGSKK 610

BLAST of Cp4.1LG02g00440 vs. ExPASy TrEMBL
Match: A0A5D3CCE3 (Putative 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001250 PE=3 SV=1)

HSP 1 Score: 993 bits (2568), Expect = 0.0
Identity = 539/616 (87.50%), Postives = 574/616 (93.18%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           M A KFNKKK+VKRDDP SKRKKKDELFTPKR K EEE+DSDLSDALD EEV++G++D+D
Sbjct: 1   MAATKFNKKKSVKRDDPPSKRKKKDELFTPKRSK-EEEDDSDLSDALDEEEVDNGLIDSD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDE--ENVSRSVSGSDSDESDIEEKSRAIDEER 120
           E+GSGSGS  E SSDGDDPLA DF  GSDE  EN S SVS SDSDE+DIE+KSR IDE+R
Sbjct: 61  EEGSGSGS--EDSSDGDDPLAHDFLQGSDEDEENDSGSVSSSDSDETDIEDKSRIIDEKR 120

Query: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKAL 180
           AREEKDAADEMQLNIK+ESDEFRLPTKEELEEE+LRPPD+SNLQRRIREIVRVLSNFKAL
Sbjct: 121 AREEKDAADEMQLNIKDESDEFRLPTKEELEEESLRPPDLSNLQRRIREIVRVLSNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKDIGSYYGYNEFL GVLVE+FPVVELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANE+K  RLKSLTANLHRMGVTN
Sbjct: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEIKAQRLKSLTANLHRMGVTN 360

Query: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420
           TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCA+LQKQL
Sbjct: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAELQKQL 420

Query: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRF 480
           ILAAIDMVD+NSKSGGY+VYSTCSIMVDENEA+VDYAL+KR+VKLVPCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDSNSKSGGYVVYSTCSIMVDENEAIVDYALRKRDVKLVPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEK 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIP+A+SSE+L+E   E+ D    K
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPSAKSSESLDEVVEEDNDT---K 540

Query: 541 KSANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600
           K  NEKNDGECLHPQ+NDG+K KK  S+ H V NG+ESKP VE+R+KRKFPSREEIS+AR
Sbjct: 541 KGGNEKNDGECLHPQLNDGEKGKKG-SQNHAVGNGKESKPSVERRQKRKFPSREEISRAR 600

Query: 601 EEKREALREKKAGGSK 614
           EEKR+ALR KK G  K
Sbjct: 601 EEKRQALRVKKHGSKK 609

BLAST of Cp4.1LG02g00440 vs. ExPASy TrEMBL
Match: A0A1S3CJU2 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103501261 PE=3 SV=1)

HSP 1 Score: 993 bits (2568), Expect = 0.0
Identity = 539/616 (87.50%), Postives = 574/616 (93.18%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           M A KFNKKK+VKRDDP SKRKKKDELFTPKR K EEE+DSDLSDALD EEV++G++D+D
Sbjct: 1   MAATKFNKKKSVKRDDPPSKRKKKDELFTPKRSK-EEEDDSDLSDALDEEEVDNGLIDSD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDE--ENVSRSVSGSDSDESDIEEKSRAIDEER 120
           E+GSGSGS  E SSDGDDPLA DF  GSDE  EN S SVS SDSDE+DIE+KSR IDE+R
Sbjct: 61  EEGSGSGS--EDSSDGDDPLAHDFLQGSDEDEENDSGSVSSSDSDETDIEDKSRIIDEKR 120

Query: 121 AREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKAL 180
           AREEKDAADEMQLNIK+ESDEFRLPTKEELEEE+LRPPD+SNLQRRIREIVRVLSNFKAL
Sbjct: 121 AREEKDAADEMQLNIKDESDEFRLPTKEELEEESLRPPDLSNLQRRIREIVRVLSNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKDIGSYYGYNEFL GVLVE+FPVVELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANE+K  RLKSLTANLHRMGVTN
Sbjct: 301 VMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEIKAQRLKSLTANLHRMGVTN 360

Query: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420
           TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCA+LQKQL
Sbjct: 361 TVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAELQKQL 420

Query: 421 ILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRF 480
           ILAAIDMVD+NSKSGGY+VYSTCSIMVDENEA+VDYAL+KR+VKLVPCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDSNSKSGGYVVYSTCSIMVDENEAIVDYALRKRDVKLVPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEK 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIP+A+SSE+L+E   E+ D    K
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPSAKSSESLDEVVEEDNDT---K 540

Query: 541 KSANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600
           K  NEKNDGECLHPQ+NDG+K KK  S+ H V NG+ESKP VE+R+KRKFPSREEIS+AR
Sbjct: 541 KGGNEKNDGECLHPQLNDGEKGKKG-SQNHAVGNGKESKPSVERRQKRKFPSREEISRAR 600

Query: 601 EEKREALREKKAGGSK 614
           EEKR+ALR KK G  K
Sbjct: 601 EEKRQALRVKKHGSKK 609

BLAST of Cp4.1LG02g00440 vs. ExPASy TrEMBL
Match: A0A6J1FCL8 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442793 PE=3 SV=1)

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 543/616 (88.15%), Postives = 571/616 (92.69%), Query Frame = 0

Query: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLDAD 60
           M AAKFNKKK VKRDD   K KKKDELFT KR + EE++DS++SD +D+EEVE+G+LD D
Sbjct: 1   MAAAKFNKKKVVKRDDLPLKGKKKDELFTRKRSQ-EEDDDSEISDGMDMEEVENGVLDGD 60

Query: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSD-EENVSRSVSGSDSDESDIEEKSRAIDEERA 120
           E+GS SGS  EVSSDGDDPLADDF  GSD EEN S S SGSDSDE DIE KSR IDEERA
Sbjct: 61  EEGSESGS--EVSSDGDDPLADDFLQGSDDEENGSGSDSGSDSDE-DIENKSRIIDEERA 120

Query: 121 REEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLSNFKALR 180
           REEKDAADEMQLNI+EESDEFRLPTKEELEEEAL PPD+SNLQRRIREIVRVL+NFKALR
Sbjct: 121 REEKDAADEMQLNIREESDEFRLPTKEELEEEALGPPDLSNLQRRIREIVRVLTNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKD+ SYYGYNEFL GVLVE+FPVVELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDLCSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNT 360
           MALAPQEKERI+DMAAAPGGKTTYIAALMKNSG+IFANEMK PRLKSLTANL RMGVTNT
Sbjct: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGLIFANEMKAPRLKSLTANLQRMGVTNT 360

Query: 361 VVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLI 420
           VVCNYDGKELPKVLG NTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLI
Sbjct: 361 VVCNYDGKELPKVLGKNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLI 420

Query: 421 LAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFR 480
           LAAIDMVD+NSKSGGY+VYSTCSIMVDENEAVVDYALKKR+VKL PCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMVDSNSKSGGYVVYSTCSIMVDENEAVVDYALKKRDVKLAPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDFEAEKK 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAKL+KMSNKIPTAQSSE LEE A  EED E+EK 
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLQKMSNKIPTAQSSELLEETA--EEDSESEK- 540

Query: 541 SANEKNDGECLHPQVNDGKKRKKVVSEKHEVENGEE-SKPGVEKRKKRKFPSREEISKAR 600
           S NEK+DGECLHP+V+DGKKRKKV S+ H+ ENGEE SKP VEKRKKRKFPSREEISKAR
Sbjct: 541 SGNEKDDGECLHPKVSDGKKRKKV-SKNHDDENGEEESKPTVEKRKKRKFPSREEISKAR 600

Query: 601 EEKREALREKKAGGSK 614
           EEKR+ALREKK G  K
Sbjct: 601 EEKRQALREKKTGSKK 608

BLAST of Cp4.1LG02g00440 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 679.1 bits (1751), Expect = 3.3e-195
Identity = 396/641 (61.78%), Postives = 477/641 (74.41%), Query Frame = 0

Query: 9   KKAVKRDDPQSKRK----------KKDELFTPKRGKKEEENDSDLSDALDVEEVEDGMLD 68
           KKA K   P  K++          KK E+ T +  ++EE   SD       E   D   D
Sbjct: 29  KKAAKTQKPPLKKQRKCISEKKPLKKPEVSTDEEEEEEENEQSDEGS----ESGSDLFSD 88

Query: 69  ADEQGSGSGSGSEVSSDGDD-------PLADDFFHGSDEENVSR-SVSGSDSDESDIEEK 128
            DE+G+      +   D DD       PLA+DF  GSD E V+  S   SDS  S +E K
Sbjct: 89  GDEEGNNDSDDDDDDDDDDDDDDEDAEPLAEDFLDGSDNEEVTMGSDLDSDSGGSKLERK 148

Query: 129 SRAIDEERAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVR 188
           SRAID +R +E +DA DE ++NIKE+ DEF+LPT++ELEEEA RPPD+ +LQ RIREIVR
Sbjct: 149 SRAIDRKRKKEVQDADDEFKMNIKEKPDEFQLPTQKELEEEARRPPDLPSLQMRIREIVR 208

Query: 189 VLSNFKALRQEG--ATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEK 248
           +LSNFK L+ +G    R DYV QLK D+ SYYGYNEFL G L+E+FPVVELMELIEAFEK
Sbjct: 209 ILSNFKDLKPKGDKHERNDYVGQLKADLSSYYGYNEFLIGTLIEMFPVVELMELIEAFEK 268

Query: 249 PRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFY 308
            RP  +RTNTLKTRRRDLAD+L+NRGVNLDPLSKWSKVGL+VYDSQVPIGATPEY+AGFY
Sbjct: 269 KRPTSIRTNTLKTRRRDLADILLNRGVNLDPLSKWSKVGLIVYDSQVPIGATPEYLAGFY 328

Query: 309 MLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLT 368
           MLQ ASSFLPVMALAP+EKER+VDMAAAPGGKTTY+AALMKN+GII+ANEMKVPRLKSL+
Sbjct: 329 MLQSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNTGIIYANEMKVPRLKSLS 388

Query: 369 ANLHRMGVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEI 428
           ANLHRMGVTNT+VCNYDG+EL KVLG ++ DRVLLDAPCSG+GV+SKDESVKTSKS ++I
Sbjct: 389 ANLHRMGVTNTIVCNYDGRELTKVLGQSSVDRVLLDAPCSGTGVISKDESVKTSKSADDI 448

Query: 429 QKCAQLQKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGL 488
           +K A LQKQLIL AID+VDANSK+GGYIVYSTCS+M+ ENEAV+DYALK R+VKLVPCGL
Sbjct: 449 KKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSVMIPENEAVIDYALKNRDVKLVPCGL 508

Query: 489 DFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSE---ALE 548
           DFGRPGF  FRE RFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSN +  + + E    +E
Sbjct: 509 DFGRPGFSSFREHRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNAMQPSGNDEPAVTME 568

Query: 549 EEAV---EEEDFEAE---------------KKSANEKND-GECLHPQVNDGKKRKKVVSE 608
           +  V   +++D +AE               K+ +N K D  +  +P+  +  K K+  + 
Sbjct: 569 QAQVSSSDDDDEKAEAIEELEKPPVASGQPKRESNTKEDTNKRKNPRSKEIHKGKRNKNT 628

BLAST of Cp4.1LG02g00440 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 664.1 bits (1712), Expect = 1.1e-190
Identity = 397/652 (60.89%), Postives = 474/652 (72.70%), Query Frame = 0

Query: 2   PAAKFNKKKAVKRDDPQSKRKKK---------DELFTPKRGKKEEEND----SDLSDALD 61
           P     KK A  ++ P  K++KK         D   T    + +E +D     D+S A++
Sbjct: 33  PQTSMLKKGAKSQNKPPLKKQKKEVVEEEPLEDYEVTDDSDEDDEVSDGSDEDDISPAVE 92

Query: 62  VEEVEDGMLDADEQGSGSGSGSEVSSDGDDPLADDFFHGS---DEENVSRSVSGSDSDES 121
            EE+++   D  E GS      +   + ++ L DDF  GS   DEE    + S +DSD+ 
Sbjct: 93  SEEIDES--DDGENGSNQLFSDDEEENDEETLGDDFLEGSGDEDEEGSLDADSDADSDDD 152

Query: 122 DIEEKSRAIDEERAREEKDAADEMQLNIKE--------ESDEFRLPTKEELEEEALRPPD 181
           DI  KS AID + A ++KDAA E++  IK+        E D FRLPT+EELEEEA  PPD
Sbjct: 153 DIVAKSDAIDRDLAMQKKDAAAELEDFIKQDDVHDEEPEHDAFRLPTEEELEEEARGPPD 212

Query: 182 ISNLQRRIREIVRVLSNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVV 241
           +  L+ RI EIVR L NFKA R +  TRK  VEQLK D+GSYYGYN FL G LVE+FP  
Sbjct: 213 LPLLKTRIEEIVRALKNFKAFRPKDTTRKACVEQLKADLGSYYGYNSFLIGTLVEMFPPG 272

Query: 242 ELMELIEAFEKPRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPI 301
           ELMELIEAFEK RP  +RTNTLKTRRRDLADVL+NRGVNLDPLSKWSKVGLV+YDSQVPI
Sbjct: 273 ELMELIEAFEKQRPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPI 332

Query: 302 GATPEYMAGFYMLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFAN 361
           GATPEY+AG+YMLQGASSFLPVMALAP+E ERIVD+AAAPGGKTTYIAALMKN+G+I+AN
Sbjct: 333 GATPEYLAGYYMLQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYAN 392

Query: 362 EMKVPRLKSLTANLHRMGVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVVSKDE 421
           EMKVPRLKSLTANLHRMGVTNT+VCNYDG+ELPKVLG NT DRVLLDAPCSG+G++SKDE
Sbjct: 393 EMKVPRLKSLTANLHRMGVTNTIVCNYDGRELPKVLGQNTVDRVLLDAPCSGTGIISKDE 452

Query: 422 SVKTSKSLEEIQKCAQLQKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALK 481
           SVK +K+++EI+K A LQKQL+LAAIDMVDANSK+GGYIVYSTCSIMV ENEAV+DYALK
Sbjct: 453 SVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYALK 512

Query: 482 KRNVKLVPCGLDFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT 541
           KR+VKLV CGLDFGR GF RFRE RF PSL+KTRRFYPHVHNMDGFFVAKLKKMSN    
Sbjct: 513 KRDVKLVTCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSN---V 572

Query: 542 AQSSEALEEEAVE---------EEDFEAEKKSANEKNDGECLHPQVNDGKKRKKVVSEKH 601
            QSSE  +++AVE         ++D EAE     EK       P+    KK K+ +++  
Sbjct: 573 KQSSEEGDDDAVETVEQAEVSSDDDDEAEAIEETEKPSVPVRQPKERKEKKNKEKLAKSK 632

Query: 602 EVENG------------EESKPGVEKRKKRKFPSREEISKAREEKREALREK 609
           E + G            E SKP  +K+K+R++  + EI++AREEKR A+REK
Sbjct: 633 EDKRGKKDKKSKSENVEEPSKPRKQKKKRREW--KNEIAQAREEKRIAMREK 677

BLAST of Cp4.1LG02g00440 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 106.7 bits (265), Expect = 6.8e-23
Identity = 82/232 (35.34%), Postives = 123/232 (53.02%), Query Frame = 0

Query: 286 GFYMLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLK 345
           G   +Q  S+ L V  + PQ  ERI+D  AAPGGKT ++A+ +K  G+I+A ++   RL+
Sbjct: 310 GICSVQDESAGLIVSVVKPQPGERIMDACAAPGGKTLFMASCLKGQGMIYAMDVNEGRLR 369

Query: 346 SL--TANLHRM-GVTNTVVCNYDGKELPKVLGMNTA--DRVLLDAPCSGSGVVSKDESVK 405
            L  TA  H++ G+  T+       +L      N    D+VLLDAPCSG GV+SK   ++
Sbjct: 370 ILGETAKSHQVDGLITTI-----HSDLRVFAETNEVQYDKVLLDAPCSGLGVLSKRADLR 429

Query: 406 TSKSLEEIQKCAQLQKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRN 465
            ++ LE++ +  +LQ +L+ +A  +V    K GG +VYSTCSI  +ENE  V+  L +  
Sbjct: 430 WNRKLEDMLELTKLQDELLDSASKLV----KHGGVLVYSTCSIDPEENEGRVEAFLLR-- 489

Query: 466 VKLVPCGLDFGRPGFIRFREKRFHPSLEKTRRFY----PHVHNMDGFFVAKL 509
                       P F       F PS   T + +    P  H++DG F A+L
Sbjct: 490 -----------HPEFTIDPVTSFVPSSFVTSQGFFLSNPVKHSLDGAFAARL 519

BLAST of Cp4.1LG02g00440 vs. TAIR 10
Match: AT1G06560.1 (NOL1/NOP2/sun family protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.7e-13
Identity = 88/328 (26.83%), Postives = 125/328 (38.11%), Query Frame = 0

Query: 284 MAGFYMLQGASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFA---NEMK 343
           + G   LQ   S +   AL PQ+ ERI+DM AAPGGKTT IA LM + G I A   +  K
Sbjct: 274 LEGEIFLQNLPSIIVAHALDPQKGERILDMCAAPGGKTTAIAILMNDEGEIVAADRSHNK 333

Query: 344 VPRLKSLTANLHRMGVTNTVVCNYDGKE---LPKVLG----------------------- 403
           V  +++L+A    MG T    C  D  +   LP  L                        
Sbjct: 334 VLVVQNLSA---EMGFTCITTCKLDALKSVCLPTTLNESTILINGDNSSSMTSHSELSSN 393

Query: 404 ------------------------------------------------------------ 463
                                                                       
Sbjct: 394 EEMTSVTSRRSEADKSCEKNDSTEQPNGGDNVSQAYIRKNKGRLKNGRGRTQCQGGRAGK 453

Query: 464 -----MNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQLILAAIDMVDAN 508
                 N+ DRVLLDAPCS  G+  +       +++  ++     Q++++  A+ +V   
Sbjct: 454 SQGFPPNSFDRVLLDAPCSALGL--RPRLFAGLETVVSLRNHGWYQRKMLDQAVQLV--- 513

BLAST of Cp4.1LG02g00440 vs. TAIR 10
Match: AT4G17590.1 (CONTAINS InterPro DOMAIN/s: Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p (InterPro:IPR001678); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G26600.1); Has 459 Blast hits to 368 proteins in 186 species: Archae - 2; Bacteria - 0; Metazoa - 137; Fungi - 144; Plants - 100; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 69.7 bits (169), Expect = 9.2e-12
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 0

Query: 332 GIIFANEMKVPRLKSLTANLHRMGVTNTVVCNYD-GKELPKVLGMNTADRVLLDAPCSGS 391
           GIIFAN      L SL ANLHRMG+TNTVV NY+   +L +V  +N+ D VL++AP + +
Sbjct: 55  GIIFANASTEHLLGSLYANLHRMGITNTVVSNYNINTKLSRVFHINSKDMVLVNAPSTRT 114

Query: 392 GVVSKDESVKTSKSLE-EIQKCAQLQKQLILAAIDMVD 428
           G++S+  S+K S + E +IQ+   LQK  I+  + M D
Sbjct: 115 GLISEFGSIKMSINEEADIQRFGVLQK--IVEGVHMSD 150