Cp4.1LG01g26330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g26330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionHistidine--tRNA ligase
LocationCp4.1LG01: 21215113 .. 21222071 (-)
RNA-Seq ExpressionCp4.1LG01g26330
SyntenyCp4.1LG01g26330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACCCTGCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCATCCATGCCATTGCCCATGGCTTTGCTCTGGTTCGGATCGACTCATCAGCCCTCGAGAGGCTTGCATCTTCGTCGAACAATCGAAATGCTGCATCCGTCAAGCACCAAGTCGTCATTCCTGATTACCTGACTAGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTGAACAAGCTCATCATCTCTAGTTCTTCCGGTATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAATTCAAACCCAGAAACTTTACAATTTGAGTCACTTGATGTGACCGACGAGGAGTTATCCGTGTTCAAGCAATCGTGTTATGTTTTGAACGGAGTTTGCGCGTTGTTAGACCATCAATCAGCAGCATTATCCTCCATTGCTGATGCTGTGGCGAGCATATCTTGCGAGGCTTCTAGAGCTGACGTGTCTGTGTTCAATTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCTAATGATATGAAGGTATTACTAAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTATTTCCAAGATCCCAAAGAATCACGGATGCTTGAGAGAGCAAGCTAAGTTGGTGCATTCGAAAATGCGGGTTGAGTTGAATTCTAATGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACCGAGGATACCGTTCGGACCGCATTGTTGTCATTTGCAGCCATGCTGTGGGACTTGGGAAAATGTAGTTTGGATAGAGGAAAACTGATTTTGGATTCAAATAGCGATGATAATATGAAGGCGAGTTTGGCGGGTTTGCTTGATAGGGAATGTCCCAGTAATGAGAGTCTCAAAAAGGTTTATAGGTTGGTCTCCGATTTAGCTTTGGATGACAATTATGATGAGTTTGTCCATACTGTCAATGTGTTGTTGTTAACTGTATGGAAAATTGTCGTTTGGGAGGCAATAGCAGGCTTCTTAACGATTGAAGGCGGTAAATTGATAGGAAAGGGTCAAGATGTTGACATGAATCGAGCTAATGAAAAGGTGGTAAATAAGAGTGAGAAGAAGAAGAAACNGGAAAGGGTCAAGATGTTGACATGAATCGAGCTAATGAAAAGGGGGTAAATAAGAGTGAGAAGAAGAAGAAAGCAGTCTTGGGTAAAGGAACTAGCGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAAAGGTGGTGATCTGGGGTTGGAGAACTCAGTAAAAGATCTTTTATCCTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGATGAAAATCAGAGAGATTGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGTAATACCGTACTTCAGAACTTTACCTAAATTCCAAGCCATTTAGCCATCCTCCCTTTGTTTTGGTCTAGGAAGTGGAATCTCATTAGAAGAATAGTGGCATCCTTTTTTCATGGCTCCTGATTCCTTTTGTCTTCAATTGCATGCCGCTACATAGTTTAGTAACGGTAATAATGAAATCGTCCTTTTACTATCAGTTTAAAAATGCTAAATGTATTGCCTTTACTAAATATTCCTACAATACTACATAAAACATATACCTTAAACTTACCATTACCATCCATTTTATATTTTAATTCTTTGGTCCATCAAAGAAATTGTTTCCTATCAAAAAAAAAAAAAAAATCTTTGATATATTCTTTATAATCATTGTTATTAACGTAGGTATTTTATACAGCCTTATATCAGTTGAAATTGTTGTTACATGTAGACATGATAGTTACTCACAAACCAAATTACTGAAAAGTGATGATTCAATTTTTCTGGCTGCAAATTTTTTATATTATGCTTCATTACAGTAGTTTATTTTGAAACCTTGGCGTGCAGTAGTTGGCTTGTGCATGCGTCAGGTTTCTATGAAAGGAGAAAAAAAAGGAAGACACTATTTTCTTTTTAACACTAAACTTCTCCTTCTTCTTCTTCCACTTCCACTTTTTGGATAAGAATCCAACTTTCATTGAGAGTGCTTACTTCTGGTTTTCTATGTAAAGTAAAGAGAATAACAATAGTTTTCTTTTTCTGAAATCACTAATCAGTATGTTATCTTTGTCTATTTGAGAAGTCTCAATTTAGAAAAGAAGATAGAAAGTGAGTCATTCCTTTTCATTTGGAAGGATACAGCTACAGGATTAGGCTTATTTTGGTTTTCTAAATCTATTTCATGCTTTTGTTAATACTGTTATCTTTTGATTTGATTTAATATTTTATTGTTCCCTCTTTCTAACTTGAGTAAGGTGTTTGTTACTTTAACTCAACTTAATGTTCTTACATATTAGGGCACTCGGGATTTTGCAAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGCCATGGTGCCACAGCCTTGGATACTCCGGCTTTTGAGTTGAGAGAAACACTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGTCTGATCAGGTTATAGTAGATGTTATTACTTGTTTTAACTGTTAGCAGACACTACCATTGTTTCTCTAATCAAGTTTTTTAGACATAAATTGTTGGGATATTTATCCCAATTTATTTTCTTCCTTTATTCTGNGACATAAATTGTTGGGATATTTATCCCAATTTATTTTCTTCCTCTTTATTCTCAGCGTCTATTCATGCTTATTCATGCTTCATGAAATAATTACATGATTAACGATCCACTAAAAATATTCTGATCTGTTGTTGAGCATAATGGTTAGTTGACTGTTGAAATTTTTGGAATATTATTTATTTTTAATAGTTCTTGTATCTGTTGGCTTATTAATGCCATTTATACTAGCATTTGCATTGATATATGATGCATCGTGGAGCTTTTCTGAGCATCTTAACATGCTATTATCACGTCAAGGTTCTCTAATTTTTTGGGTATTATGTATGTTATTCATTTTATTTGTTTGTTATTGGATTAATGCTTCTCTGTTTATTCTGTGACTGTCTAGCTCTCTCTATTCTATCAAGTTTTATGATTGTTGGAATAGTTATGTGACAGAGGAATAACTTCTTGTTCCTTTTTTGTTCTCCATCATTGTAATTTCTTTTTCAGTCTGTTTACAGGGAGGGGAGCTCTGTTCGCTTAGATATGACTTGACTGTTCCGTTTGCTAGGTACGTTGCCATGAATGGTATAACATCATTCAAAAGGTATCAAATAGCAAAAGTTTATAGAAGGGACAACCCATCTAAGGGAAGATTCCGTGAATTTTATCAGTGTGATTTTGATATTGCTGGTCAATATGAAAAGATGGGACCAGATTTTGAGGTTATTAAGATACTAACGGAGCTACTTGATGAGCTGAACATTGGACAGTATGAGGTAGCCTTTTTTACTAATTGTTCTGCATAATATAACTCAATGTATTTGTTTGCTTGTTTAATTTTTATAAATCTGTAGTAATTTTGAATAATATGCTGAATCAGTAAGTATAGCACAAAACATCAAGCAATATATATTAAGCACAGTTTCCCATATTATCATATTGCCTGAGTGGACTGGATTCCTGGATTCCTTTCTATATCTCGGGGTTGCTCAGTCAATTCGTGTTGTTACTGCTATTTATTTCTGACACCACTTTGTGGTTAAACTTTTAGCCTAATCTATATTTTATACATTTTCTTCTCAGATAAAATTGAACCATCGAAAGCTGTTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAGTCTTTTGACCAGATAAAAAGAGAAATGGTAATTAAATTTACTGTTGACCACAATTTTATTATTTATTGAGCATGCCTGCCTCTTTCTGCCTTACCCTCTTAGTTTCTTTTGGTTATCTGATTTATTTTGATTGCAGATTACGTTCGCCAATTTTAAAATGTGGTGGTAACATATATTTGCATAATTGCATTTCACTCAAGTCAAAGCTCTTTTCATTTTGGTTATTATCATTTGTTTAGTTTTTTTTTTTTCTAATTTGATTTTGTTTTTAGTTTGTTAACTTAATTTCCATTGCAAGGTAACTGTTACATTTAATCCGTTTTCCTAGTTTTGCATTTTTTGTCTTCCATCTAGCCATAACAAGGATGGATTTATTATACTTAATTTTATAGTAATGTTTACTTGCCAGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAACAGGAGCAGAGCCCATTATTACAAAATAAAGGATCCTCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCTAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGGTAAGTTTCTATATGTCATCATCATGTTGGTTGGTANTTAGTTTGTTAACTTAATTTCCATTGCAAGGTAACTGTTACATTTAATCCGTTTTCCTAGTTTTGCATTTTTTGTCTTCCATCTAGCCATAACAAGGATGGATTTATTATACTTAATTTTATAGTAATGTTTACTTGCCAGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAGCAGGAGCAGAGCCCATTATTACAAAATAAAGGATCATCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCAAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGGTAAGTTTCTATATGTCATCATCATGTTGGTTGGTACATCCCTTGCTTTTGACTTGTTATGCATATGCTGTTGTTTGATATTAATTTGAACTCTGCTTCAATTGCTTGAGATTTTTCCTTGATAATTCAACTACCAGTTATTGGGGGGATTTTGTTGTGTTAGAGCAATCAATGAACTCAACTTTCTGTTATTTTGAAGAATTGCTAGATATACAATCTTGCACAGTTAGTCTGGGAGCCTGGCCCTTCCTTTCGAAAGAAATATTATTTGATATCCCCCTCTGACTTCTTCTGTGAACTGCTTTTGAAAATTATACGTGGAAATTTTAAAATAGAAAAATTTGAGATAGATGGTTGCTTTCATATCCAGAATAATCTAGTTATGCAGCTATGATACTTTACTGACATCTGACGGAACTTATTTTGCTCTCTAAGATCATTTCTCCTTAGTTCTATTACCTGCAATGATGTACGAAGCTATCATCATGTTACCTTGCTGCCTCCTAATACGTATTTTATTTGGACGCTTTGAGAGGATGTAATTAACTGATCATTTATGTTCGCAGGTTGGTTCTATTGCTGCTGGTGGACGCTATGACAACCTTATTGGAATGTTTGGTTCCAAGCAGGTCCCAGCAGTTGGCGTCAGTCTTGGAATTGAACGAGTTTTTGTTATTATGGAACAACTTTTGAAAGATCAAAATCAGGTAATAACTTATCATTTTTATTTGAATCCTTTGTTTCGTGTGAGATGCGTGCTTCTCTTGTAGTTGAATGTTAATCAGACTTGAGCTTCTAAGTCATTACAGTTTTCCATAAAGCCCGCTTGTTAAGGTTCTTGGATAAAAATTCCTAGGTAATGTTGATAGTTGGTCTTGATTAATCTTTTTCCTGGTCTAGTCTTGGGGGTGAATCAATTCATCTTCATTGCTGGGTATTATTTTACCCTCCAATACAATAGGAGCACCCTCTCTAAAACTTTTATAATTATTGTCCAGCAACTATCTCTGTTAGTTGGGGTGCTCTCCCTTTTGAACTCCTGGTGGTTTTCTGAGAGTTCTCAACCTTCCATATCTAGCCGATTGATGATAAAATTTGAACTCTTCTACTTGTCTTTGTCAAGGGACTATTCATTGAATTCAACTGATGGGATTTTGCAGACTACTCGGGCCACTAAGACTGAGATTTTAGTGAGCATATTGGGAGATGATTTAACACAAGCTGCGGAGCTTGCAAGTGAGATATGGAGTGCGAAACTGAATGCAGAGTTCCTGATCAATAAAAGAGTGATGAAACATATTGATCGTGCAAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTCTGAGAAGCAATATCATTGATGAACTGAAGAAGCGTTTGGCCCCTTGATGTCATTGCTGTTGCAAAATGCAGAGCTTGTCTTCTTCAATCCCACTTCAGGAAGAACTACTTTTTCNAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTCTTAGAAGCAGTATCATTGATGAACTGAAGAAGCGTTTGGCCCCTTGATGTCATTGCTGTTGCAAAATGCAGAGCTTGTCTTCTTCAATCCCACGTCAGGAAGAACTACTTTTTCGCTGACTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATACTGAGATCTTACATACACAAGTGTTCTAATACACAGCCTTGGTTGGTATATATGTGTGTGCGCGCAAGAATTTAGTGTGTGGCTCACATTTGGGCTGTACTCGACTGACACCCAATCCATTTTTGTAATCCATTTTGCTTGTGAACTTGTTGACATAGATGGCTGAG

mRNA sequence

ATGGCGGACCCTGCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCATCCATGCCATTGCCCATGGCTTTGCTCTGGTTCGGATCGACTCATCAGCCCTCGAGAGGCTTGCATCTTCGTCGAACAATCGAAATGCTGCATCCGTCAAGCACCAAGTCGTCATTCCTGATTACCTGACTAGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTGAACAAGCTCATCATCTCTAGTTCTTCCGGTATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAATTCAAACCCAGAAACTTTACAATTTGAGTCACTTGATGTGACCGACGAGGAGTTATCCGTGTTCAAGCAATCGTGTTATGTTTTGAACGGAGTTTGCGCGTTGTTAGACCATCAATCAGCAGCATTATCCTCCATTGCTGATGCTGTGGCGAGCATATCTTGCGAGGCTTCTAGAGCTGACGTGTCTGTGTTCAATTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCTAATGATATGAAGGTATTACTAAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTATTTCCAAGATCCCAAAGAATCACGGATGCTTGAGAGAGCAAGCTAAGTTGGTGCATTCGAAAATGCGGGTTGAGTTGAATTCTAATGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACCGAGGATACCGTTCGGACCGCATTGTTGTCATTTGCAGCCATGCTGTGGGACTTGGGAAAATGTAGTTTGGATAGAGGAAAACTGATTTTGGATTCAAATAGCGATGATAATATGAAGGCGAGTTTGGCGGGTTTGCTTGATAGGGAATGTCCCAGTAATGAGAGTCTCAAAAAGGTTTATAGGTTGGTCTCCGATTTAGCTTTGGATGACAATTATGATGAGTTTGTCCATACTGTCAATGTGTTGTTGTTAACTGTATGGAAAATTGTCGTTTGGGAGGCAATAGCAGGCTTCTTAACGATTGAAGGCGGTAAATTGATAGGAAAGGGTCAAGATGTTGACATGAATCGAGCTAATGAAAAGGTGGGGGTAAATAAGAGTGAGAAGAAGAAGAAAGCAGTCTTGGGTAAAGGAACTAGCGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAAAGGTGGTGATCTGGGGTTGGAGAACTCAGTAAAAGATCTTTTATCCTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGATGAAAATCAGAGAGATTGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGGCACTCGGGATTTTGCAAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGCCATGGTGCCACAGCCTTGGATACTCCGGCTTTTGAGTTGAGAGAAACACTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGTCTGATCAGATAAAATTGAACCATCGAAAGCTGTTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAGTCTTTTGACCAGATAAAAAGAGAAATGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAACAGGAGCAGAGCCCATTATTACAAAATAAAGGATCCTCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCTAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAGCAGGAGCAGAGCCCATTATTACAAAATAAAGGATCATCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCAAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGACTACTCGGGCCACTAAGACTGAGATTTTAGTGAGCATATTGGGAGATGATTTAACACAAGCTGCGGAGCTTGCAAGTGAGATATGGAGTGCGAAACTGAATGCAGAGTTCCTGATCAATAAAAGAGTGATGAAACATATTGATCGTGCAAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTCTGAGAAGCAATATCATTGATGAACTGAAGAAGCGTTTGGCCCCTTGATGTCATTGCTGTTGCAAAATGCAGAGCTTGTCTTCTTCAATCCCACTTCAGGAAGAACTACTTTTTCNAACATACTGAGATCTTACATACACAAGTGTTCTAATACACAGCCTTGGTTGGTATATATGTGTGTGCGCGCAAGAATTTAGTGTGTGGCTCACACACCCAATCCATTTTTGTAATCCATTTTGCTTGTGAACTTGTTGACATAGATGGCTGAG

Coding sequence (CDS)

ATGGCGGACCCTGCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCATCCATGCCATTGCCCATGGCTTTGCTCTGGTTCGGATCGACTCATCAGCCCTCGAGAGGCTTGCATCTTCGTCGAACAATCGAAATGCTGCATCCGTCAAGCACCAAGTCGTCATTCCTGATTACCTGACTAGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTGAACAAGCTCATCATCTCTAGTTCTTCCGGTATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAATTCAAACCCAGAAACTTTACAATTTGAGTCACTTGATGTGACCGACGAGGAGTTATCCGTGTTCAAGCAATCGTGTTATGTTTTGAACGGAGTTTGCGCGTTGTTAGACCATCAATCAGCAGCATTATCCTCCATTGCTGATGCTGTGGCGAGCATATCTTGCGAGGCTTCTAGAGCTGACGTGTCTGTGTTCAATTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCTAATGATATGAAGGTATTACTAAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTATTTCCAAGATCCCAAAGAATCACGGATGCTTGAGAGAGCAAGCTAAGTTGGTGCATTCGAAAATGCGGGTTGAGTTGAATTCTAATGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACCGAGGATACCGTTCGGACCGCATTGTTGTCATTTGCAGCCATGCTGTGGGACTTGGGAAAATGTAGTTTGGATAGAGGAAAACTGATTTTGGATTCAAATAGCGATGATAATATGAAGGCGAGTTTGGCGGGTTTGCTTGATAGGGAATGTCCCAGTAATGAGAGTCTCAAAAAGGTTTATAGGTTGGTCTCCGATTTAGCTTTGGATGACAATTATGATGAGTTTGTCCATACTGTCAATGTGTTGTTGTTAACTGTATGGAAAATTGTCGTTTGGGAGGCAATAGCAGGCTTCTTAACGATTGAAGGCGGTAAATTGATAGGAAAGGGTCAAGATGTTGACATGAATCGAGCTAATGAAAAGGTGGGGGTAAATAAGAGTGAGAAGAAGAAGAAAGCAGTCTTGGGTAAAGGAACTAGCGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAAAGGTGGTGATCTGGGGTTGGAGAACTCAGTAAAAGATCTTTTATCCTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGATGAAAATCAGAGAGATTGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGGCACTCGGGATTTTGCAAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGCCATGGTGCCACAGCCTTGGATACTCCGGCTTTTGAGTTGAGAGAAACACTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGTCTGATCAGATAAAATTGAACCATCGAAAGCTGTTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAGTCTTTTGACCAGATAAAAAGAGAAATGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAACAGGAGCAGAGCCCATTATTACAAAATAAAGGATCCTCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCTAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGGTGGAGGAAAAAGGATTGGCCCTGGAGATAGCTGAAAGAATAGGTGATTTTGTGAAGGAAAGGGGACATCCTTTGGAGTTACTAGCTAAGCTTAAGCAGGAGCAGAGCCCATTATTACAAAATAAAGGATCATCTGGTGCATTGAGTGAATTAGAGATTTTATTTCGTGCTCTAGAAAAGGCAAAGTGTGTTGACAAAGTGGTCTTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGTACCCAGACTACTCGGGCCACTAAGACTGAGATTTTAGTGAGCATATTGGGAGATGATTTAACACAAGCTGCGGAGCTTGCAAGTGAGATATGGAGTGCGAAACTGAATGCAGAGTTCCTGATCAATAAAAGAGTGATGAAACATATTGATCGTGCAAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTCTGAGAAGCAATATCATTGATGAACTGAAGAAGCGTTTGGCCCCTTGA

Protein sequence

MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQVVIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEELSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKEEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKGGSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDVDMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLSDQIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP
Homology
BLAST of Cp4.1LG01g26330 vs. ExPASy Swiss-Prot
Match: F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 3.6e-197
Identity = 432/938 (46.06%), Postives = 561/938 (59.81%), Query Frame = 0

Query: 5   AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQVV-IP 64
           AE S ITLGGKGSSLSSSS++ +A G A VRIDSSA+ER ++    RN  S+K     IP
Sbjct: 3   AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFST----RNVPSIKRSSFGIP 62

Query: 65  DYLTSEEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSNPETLQFESLDVTD 124
             LT+EE RASL VLLNKLI+S     SSS  RSV+P+ I E LN   E+L+   +DVT+
Sbjct: 63  QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122

Query: 125 EELSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFAS 184
            E  V ++SC  L G+C+++DH+S  LS I D+VA++SCE ++AD++ F+L+DSGDG   
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182

Query: 185 KEEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIG 244
           K+ +GVA D+KVLLNG K  GK+E   ISKIP  HG  R   K VH+  R ELNS VK G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242

Query: 245 KGGSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 304
           K GS ++G  + + T LL     + +LG CS  R KL  +   D+N+K  L+   ++ C 
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKICV 302

Query: 305 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKL-IGKG 364
            NE+LK  Y+L     L+++Y  F H +N  L  VW+IV  EA+A F  + GG+L + K 
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362

Query: 365 QDVDMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRL----QGKGGDL--GLENSVK 424
            D D  +   K    K + +KKAV+GKGTS+V+Q IKDRL        GD    L    +
Sbjct: 363 GDAD--KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGE 422

Query: 425 DLLSFLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGV 484
            +L+  +P+   FD+LL K++EIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ V
Sbjct: 423 QILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNV 482

Query: 485 FERHGATALDTPAFELRETLMGKYGEDSKLIYDLSDQ----------------------- 544
           F+RHGATALDTP FELRETLMGKYGEDSKL+YD++DQ                       
Sbjct: 483 FKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNG 542

Query: 545 ------------------------------------------------------------ 604
                                                                       
Sbjct: 543 ITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIG 602

Query: 605 ---IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAE 664
              +KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA+
Sbjct: 603 DYEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIAD 662

Query: 665 RIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLS 724
           RIG+FVKE+G PLELL+KL+QE S  L N+ S  AL EL I+F AL+++KC +++VFDLS
Sbjct: 663 RIGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLS 722

Query: 725 LARGLDYYTGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPL 784
           LARGLDYYTGVI+EA+               I   +G                       
Sbjct: 723 LARGLDYYTGVIFEAVC--------------IGAEVGSI--------------------- 782

Query: 785 LQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRA 839
               G+ G    L  +F      K V  V   L + R  +     I E + +   Q  R 
Sbjct: 783 ----GAGGRYDNLIGMF----GTKQVPAVGMSLGIERVFN-----IMEELNEKQKQVIRP 842

BLAST of Cp4.1LG01g26330 vs. ExPASy Swiss-Prot
Match: P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)

HSP 1 Score: 479.9 bits (1234), Expect = 5.7e-134
Identity = 312/807 (38.66%), Postives = 434/807 (53.78%), Query Frame = 0

Query: 138 LDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKEEVGVANDMKVLLNGSKL 197
           +D  ++ L  +ADAVA++SCEA+R DV+ F++  SGDG ++K+E  VA D+K+LL GSKL
Sbjct: 36  IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95

Query: 198 VGK---IESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKGGSLSS--GTEDTVR 257
           VG     ++ + +K+P  +G  RE  + +H+++R+ELN+ VK+GK  ++ +  G E+ + 
Sbjct: 96  VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155

Query: 258 TALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSNESLKKVYRLVSD 317
                 A  +  + K S+ R +L +    D  ++  L             +  +  ++  
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLT--------DGVEIDDLKGMLGK 215

Query: 318 LALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDVDMNRA------- 377
           + +D +    +  V   LL    I+ WEA      IE    I K Q  + N A       
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275

Query: 378 ---NEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSV-----KDLLSFL 437
               EK   +K  KKKK  LGKGTS V+ L++D +      L + +++      +L    
Sbjct: 276 HASGEKPKGDKKSKKKK-TLGKGTSAVLMLLRDLVTNGKEVLSVNSALLAEWGTELSLLF 335

Query: 438 DPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGA 497
           DPK     +L+ K++EIVE+NE RRLPK+PKGTRDF KEQMAIR+ AFSII GVF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395

Query: 498 TALDTPAFELRETLMGKYGEDSKLIYDLSDQ----------------------------- 557
            +LDTP FELRETLMGKYGEDSKLIYDL+DQ                             
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455

Query: 558 ---------------------------------------------------------IKL 617
                                                                    IKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515

Query: 618 NHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFV 677
           NHRKLLDGMLEICGVPPEKFRT+CSSIDKLDKQ+F+Q+K+E+V+EKG++ E A++IGD V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575

Query: 678 KERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLD 737
           K RG PLE+L +L++E S  + N GS  AL+ELEILF+AL+KA  + K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635

Query: 738 YYTGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGS 797
           YYTGVIYEA+FKG TQV                +   G    L+     +Q P +     
Sbjct: 636 YYTGVIYEAVFKGTTQVGS--------------IAAGGRYDNLVGMFSGKQVPAV----- 695

Query: 798 SGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEIL 839
            G    +E +F  +E+ +     +                            RAT+TE+L
Sbjct: 696 -GVSLGIERVFAIMEQQEMEKNQI----------------------------RATETEVL 755

BLAST of Cp4.1LG01g26330 vs. ExPASy Swiss-Prot
Match: E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 3.4e-62
Identity = 169/484 (34.92%), Postives = 246/484 (50.83%), Query Frame = 0

Query: 445 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 504
           K  KGTRD+  +QMAIR+K F+II   F+RHGA  +D+P FEL+ETL GKYGEDSKLIYD
Sbjct: 57  KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116

Query: 505 LSDQ-------------------------------------------------------- 564
           L DQ                                                        
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 565 --------------------------------IKLNHRKLLDGMLEICGVPPEKFRTICS 624
                                           IK+N R++LDGM  ICGVP EKFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236

Query: 625 SIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKG 684
           ++DKLDK +++++K+EMV EKGL+ E+A+RI D+V  +G   +L  +L Q+   L Q+K 
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDPK-LSQSKQ 296

Query: 685 SSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVEEKGLALE 744
           +   ++++++LF  LE  +  DKVVFDLSLARGLDYYTGVIYEAI    TQ      A  
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAIL---TQA-NPAPAST 356

Query: 745 IAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVF 804
            AE+ G                 ++    + +    G    L  +F    K + V  V  
Sbjct: 357 PAEQNG----------------AEDAGVSVGSVAGGGRYDGLVGMFD--PKGRKVPCVGV 416

Query: 805 DLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILV-SILGDDLTQAAELASEIWSAKLN 838
            + + R        I E   +  ++  R T+T++LV S   + L +  +L +E+W+A + 
Sbjct: 417 SIGIERVFS-----IMEQKAELSSEKVRTTETQVLVASAQKNLLEERLKLTAELWNAGIK 476

BLAST of Cp4.1LG01g26330 vs. ExPASy Swiss-Prot
Match: Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 4.5e-62
Identity = 166/486 (34.16%), Postives = 241/486 (49.59%), Query Frame = 0

Query: 445 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 504
           K PKGTRD++  QMA+R+K F +I   F+RHGA  +DTP FEL+ETL GKYGEDSKLIYD
Sbjct: 57  KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116

Query: 505 LSDQ-------------------------------------------------------- 564
           L DQ                                                        
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 565 --------------------------------IKLNHRKLLDGMLEICGVPPEKFRTICS 624
                                           +K+N R++LDGM  ICGVP  KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236

Query: 625 SIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKG 684
           S+DKLDK S++++K EMV EKGLA E+A+RIGD+V++ G  + L+ +L Q+   L QNK 
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQ 296

Query: 685 SSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVEEKGLALE 744
           +   L +L++LF  L      DK+ FDLSLARGLDYYTGVIYEA+             L+
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVL------------LQ 356

Query: 745 IAERIGDFVKERGHPLELLAKLKQEQSPL-LQNKGSSGALSELEILFRAL-EKAKCVDKV 804
              R G                   + PL + +  + G    L  +F     K  CV   
Sbjct: 357 PPARAG-------------------EEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV--- 416

Query: 805 VFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILV-SILGDDLTQAAELASEIWSAK 838
                L+ G++    ++ E   +   +  R T+T++LV S     L +  +L SE+W A 
Sbjct: 417 ----GLSIGVERIFSIV-EQRLEALEEKVRTTETQVLVASAQKKLLEERLKLISELWDAG 476

BLAST of Cp4.1LG01g26330 vs. ExPASy Swiss-Prot
Match: Q61035 (Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 4.5e-62
Identity = 167/504 (33.13%), Postives = 251/504 (49.80%), Query Frame = 0

Query: 428 LMKIREIVESNESRR--LPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 487
           L+K++  +  +E ++  + K PKGTRD++  QMA+R+K F +I   F+RHGA  +DTP F
Sbjct: 38  LLKLKAQLGQDEGKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVF 97

Query: 488 ELRETLMGKYGEDSKLIYDLSDQ------------------------------------- 547
           EL+ETL GKYGEDSKLIYDL DQ                                     
Sbjct: 98  ELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYR 157

Query: 548 ---------------------------------------------------IKLNHRKLL 607
                                                              +K+N R++L
Sbjct: 158 RDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRRIL 217

Query: 608 DGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHP 667
           DGM  +CGVP  KFRTICSS+DKLDK S++++K EMV EKGLA E+A+RIGD+V++ G  
Sbjct: 218 DGMFAVCGVPDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-G 277

Query: 668 LELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVI 727
           + L+ +L Q+   L QNK +   L +L++LF  L      DK+ FDLSLARGLDYYTGVI
Sbjct: 278 VSLVEQLLQDPK-LSQNKQAVEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVI 337

Query: 728 YEAI-FKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALS 787
           YEA+  +  TQ  E+ L +      G +    G               +   KG      
Sbjct: 338 YEAVLLQMPTQAGEEPLGVGSIAAGGRYDGLVG---------------MFDPKG------ 397

Query: 788 ELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILV-SIL 838
                     K  CV        L+ G++    ++ E   +   +  R T+T++LV S  
Sbjct: 398 ---------RKVPCV-------GLSIGVERIFSIV-EQRLEASEEKVRTTETQVLVASAQ 457

BLAST of Cp4.1LG01g26330 vs. NCBI nr
Match: XP_023536635.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1350 bits (3495), Expect = 0.0
Identity = 761/926 (82.18%), Postives = 763/926 (82.40%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE
Sbjct: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEK GVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK
Sbjct: 361 DMNRANEK-GVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E + K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQLLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. NCBI nr
Match: KAG7033415.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 759/942 (80.57%), Postives = 777/942 (82.48%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIPDYLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK
Sbjct: 361 DMNRANEKV-VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQ-----EQSPLLQNK 780
           GVIYEAIFKGGTQV            IG F  ++   + +   +++     EQ    QN+
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQ 780

Query: 781 GSSGALSELEILFR---------ALEKAKCVDKVVFDLSLARG--LDYYTGVIYEAIFKG 840
             S  +  L +L           ++     +   +F LSL+    L+   G++       
Sbjct: 781 QLSLLIGVLSLLNSWWFSESSQPSISSRLMIKFELFYLSLSTDYPLNSTDGIL------- 840

BLAST of Cp4.1LG01g26330 vs. NCBI nr
Match: KAG6602728.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 757/926 (81.75%), Postives = 759/926 (81.97%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIPDYLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK
Sbjct: 361 DMNRANEKV-VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E + K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQLLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. NCBI nr
Match: XP_022954606.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1336 bits (3458), Expect = 0.0
Identity = 753/926 (81.32%), Postives = 758/926 (81.86%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIP+YLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDTVRTALLSFAA+LWDLGKCSLDRGKLI DSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKD+LSFLDPK
Sbjct: 361 DMNRANEKV-VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDILSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E + K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQLLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. NCBI nr
Match: XP_022990844.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 753/926 (81.32%), Postives = 757/926 (81.75%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIPDYLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GS+SSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV +NKSEKKKKAVLGKGTSVVVQLIKDRLQG+ GDLGLENSVKDLLSFLDPK
Sbjct: 361 DMNRANEKV-LNKSEKKKKAVLGKGTSVVVQLIKDRLQGESGDLGLENSVKDLLSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E   K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQHLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. ExPASy TrEMBL
Match: A0A6J1GRC1 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1)

HSP 1 Score: 1336 bits (3458), Expect = 0.0
Identity = 753/926 (81.32%), Postives = 758/926 (81.86%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIP+YLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDTVRTALLSFAA+LWDLGKCSLDRGKLI DSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKD+LSFLDPK
Sbjct: 361 DMNRANEKV-VNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDILSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E + K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQLLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. ExPASy TrEMBL
Match: A0A6J1JT52 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1)

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 753/926 (81.32%), Postives = 757/926 (81.75%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
           VIPDYLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSAALSSIADAVA ISCEASRADVSVFNLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GS+SSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLGLENSVKDLLSFLDPK 420
           DMNRANEKV +NKSEKKKKAVLGKGTSVVVQLIKDRLQG+ GDLGLENSVKDLLSFLDPK
Sbjct: 361 DMNRANEKV-LNKSEKKKKAVLGKGTSVVVQLIKDRLQGESGDLGLENSVKDLLSFLDPK 420

Query: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480
           ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL
Sbjct: 421 ASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATAL 480

Query: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------------- 540
           DTPAFELRETLMGKYGEDSKLIYDLSDQ                                
Sbjct: 481 DTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSFKRYQI 540

Query: 541 ------------------------------------------------------IKLNHR 600
                                                                 IKLNHR
Sbjct: 541 AKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKER 660

Query: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYT 720
           GHPLELLAKLKQEQSPLLQNKGSSGALSELEILF ALEKAKCVDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGA 780
           GVIYEAIFKGGTQV            IG F                         GS   
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRYDNLIGMF-------------------------GS--- 780

Query: 781 LSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVSI 840
                         K V  V   L + R       VI E   K   QTTRATKTEILVSI
Sbjct: 781 --------------KQVPAVGVSLGIERVF-----VIMEQHLKDQNQTTRATKTEILVSI 840

BLAST of Cp4.1LG01g26330 vs. ExPASy TrEMBL
Match: A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)

HSP 1 Score: 1237 bits (3201), Expect = 0.0
Identity = 699/932 (75.00%), Postives = 733/932 (78.65%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
            IP++LT EEARASL VLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA+ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGD-----LG-LENSVKDLL 420
           D+N  NEKV V K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG      LG LENSVKDLL
Sbjct: 361 DINEVNEKV-VKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLL 420

Query: 421 SFLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFER 480
           SFLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFER
Sbjct: 421 SFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFER 480

Query: 481 HGATALDTPAFELRETLMGKYGEDSKLIYDLSDQ-------------------------- 540
           HGATALDTPAFELRETLMGKYGEDSKLIYDL+DQ                          
Sbjct: 481 HGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITS 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 FKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYE 600

Query: 601 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIG 660
           IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IG
Sbjct: 601 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIG 660

Query: 661 DFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLAR 720
           DFVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF ALEK+KCVDKVVFDLSLAR
Sbjct: 661 DFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLAR 720

Query: 721 GLDYYTGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQN 780
           GLDYYTGVIYEA+FKGGTQV            IG F                        
Sbjct: 721 GLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMF------------------------ 780

Query: 781 KGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKT 840
            GS                 K V  V   L + R       VI E + K   QTTRATKT
Sbjct: 781 -GS-----------------KQVPAVGVSLGIERVF-----VIMEQLLKDQNQTTRATKT 840

BLAST of Cp4.1LG01g26330 vs. ExPASy TrEMBL
Match: A0A5A7T4J4 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G001410 PE=3 SV=1)

HSP 1 Score: 1202 bits (3110), Expect = 0.0
Identity = 675/927 (72.82%), Postives = 722/927 (77.89%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADP EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSAL+RL+SSSN  NAAS+KH +
Sbjct: 1   MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
            IPD+LT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETL+FESLDVT+EE
Sbjct: 61  FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQS ALSS+ADAVA ISCEAS+ADVS F+LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPK HGCLREQAKLVHS+MRVELNS+VKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDT RTALLSFAAMLWDLGKCSLDRGKLIL S+ D+N+KASLA LL RECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKK Y+LV +L+LD+ YDEFVH+VNVLL+TVWKI  WEA A  LTIEGG+L+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDLLSFLDP 420
             N ANEKV V K+EKKKKAVLGKGTSVV+QLIKDRLQG GGDLG LEN VKDLLSFLDP
Sbjct: 361 GTNEANEKV-VKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDP 420

Query: 421 KASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATA 480
           KASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 
Sbjct: 421 KASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATT 480

Query: 481 LDTPAFELRETLMGKYGEDSKLIYDLSDQ------------------------------- 540
           LDTPAFELRETL GKYGEDSKLI+DL+DQ                               
Sbjct: 481 LDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQ 540

Query: 541 -------------------------------------------------------IKLNH 600
                                                                  IKLNH
Sbjct: 541 IAKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNH 600

Query: 601 RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKE 660
           RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +E+AERIGDFVKE
Sbjct: 601 RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKE 660

Query: 661 RGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYY 720
           RGHPL+LL+KLKQEQS LLQNKGSS ALSEL+ILF ALEK+KC+DKVVFDLSLARGLDYY
Sbjct: 661 RGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYY 720

Query: 721 TGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSG 780
           TGVIYEAIFKGGTQV            IG F                         GS  
Sbjct: 721 TGVIYEAIFKGGTQVGSIAAGGRFDNLIGMF-------------------------GS-- 780

Query: 781 ALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVS 840
                          K +  V   L + R L     VI E I K   +T RATKT++LVS
Sbjct: 781 ---------------KQIPAVGISLGIERVL-----VIMEQILKDQNKTVRATKTDVLVS 840

BLAST of Cp4.1LG01g26330 vs. ExPASy TrEMBL
Match: A0A5D3C5F8 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00730 PE=3 SV=1)

HSP 1 Score: 1202 bits (3110), Expect = 0.0
Identity = 675/927 (72.82%), Postives = 722/927 (77.89%), Query Frame = 0

Query: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
           MADP EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSAL+RL+SSSN  NAAS+KH +
Sbjct: 60  MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 119

Query: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
            IPD+LT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNS PETL+FESLDVT+EE
Sbjct: 120 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 179

Query: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQS ALSS+ADAVA ISCEAS+ADVS F+LMDSGDGFASKE
Sbjct: 180 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 239

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
           EVGVANDMKVLLNGSKLVGKIESGAISKIPK HGCLREQAKLVHS+MRVELNS+VKIGKG
Sbjct: 240 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 299

Query: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
           GSLSSGTEDT RTALLSFAAMLWDLGKCSLDRGKLIL S+ D+N+KASLA LL RECPSN
Sbjct: 300 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 359

Query: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
           ESLKK Y+LV +L+LD+ YDEFVH+VNVLL+TVWKI  WEA A  LTIEGG+L+GKGQDV
Sbjct: 360 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 419

Query: 361 DMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDLLSFLDP 420
             N ANEKV V K+EKKKKAVLGKGTSVV+QLIKDRLQG GGDLG LEN VKDLLSFLDP
Sbjct: 420 GTNEANEKV-VKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDP 479

Query: 421 KASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATA 480
           KASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 
Sbjct: 480 KASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATT 539

Query: 481 LDTPAFELRETLMGKYGEDSKLIYDLSDQ------------------------------- 540
           LDTPAFELRETL GKYGEDSKLI+DL+DQ                               
Sbjct: 540 LDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQ 599

Query: 541 -------------------------------------------------------IKLNH 600
                                                                  IKLNH
Sbjct: 600 IAKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNH 659

Query: 601 RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKE 660
           RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +E+AERIGDFVKE
Sbjct: 660 RKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKE 719

Query: 661 RGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYY 720
           RGHPL+LL+KLKQEQS LLQNKGSS ALSEL+ILF ALEK+KC+DKVVFDLSLARGLDYY
Sbjct: 720 RGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYY 779

Query: 721 TGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSG 780
           TGVIYEAIFKGGTQV            IG F                         GS  
Sbjct: 780 TGVIYEAIFKGGTQVGSIAAGGRFDNLIGMF-------------------------GS-- 839

Query: 781 ALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRATKTEILVS 840
                          K +  V   L + R L     VI E I K   +T RATKT++LVS
Sbjct: 840 ---------------KQIPAVGISLGIERVL-----VIMEQILKDQNKTVRATKTDVLVS 899

BLAST of Cp4.1LG01g26330 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 689.9 bits (1779), Expect = 2.6e-198
Identity = 432/938 (46.06%), Postives = 561/938 (59.81%), Query Frame = 0

Query: 5   AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQVV-IP 64
           AE S ITLGGKGSSLSSSS++ +A G A VRIDSSA+ER ++    RN  S+K     IP
Sbjct: 3   AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFST----RNVPSIKRSSFGIP 62

Query: 65  DYLTSEEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSNPETLQFESLDVTD 124
             LT+EE RASL VLLNKLI+S     SSS  RSV+P+ I E LN   E+L+   +DVT+
Sbjct: 63  QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122

Query: 125 EELSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFAS 184
            E  V ++SC  L G+C+++DH+S  LS I D+VA++SCE ++AD++ F+L+DSGDG   
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182

Query: 185 KEEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIG 244
           K+ +GVA D+KVLLNG K  GK+E   ISKIP  HG  R   K VH+  R ELNS VK G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242

Query: 245 KGGSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 304
           K GS ++G  + + T LL     + +LG CS  R KL  +   D+N+K  L+   ++ C 
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKICV 302

Query: 305 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKL-IGKG 364
            NE+LK  Y+L     L+++Y  F H +N  L  VW+IV  EA+A F  + GG+L + K 
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362

Query: 365 QDVDMNRANEKVGVNKSEKKKKAVLGKGTSVVVQLIKDRL----QGKGGDL--GLENSVK 424
            D D  +   K    K + +KKAV+GKGTS+V+Q IKDRL        GD    L    +
Sbjct: 363 GDAD--KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGE 422

Query: 425 DLLSFLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGV 484
            +L+  +P+   FD+LL K++EIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ V
Sbjct: 423 QILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNV 482

Query: 485 FERHGATALDTPAFELRETLMGKYGEDSKLIYDLSDQ----------------------- 544
           F+RHGATALDTP FELRETLMGKYGEDSKL+YD++DQ                       
Sbjct: 483 FKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNG 542

Query: 545 ------------------------------------------------------------ 604
                                                                       
Sbjct: 543 ITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIG 602

Query: 605 ---IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAE 664
              +KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA+
Sbjct: 603 DYEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIAD 662

Query: 665 RIGDFVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLS 724
           RIG+FVKE+G PLELL+KL+QE S  L N+ S  AL EL I+F AL+++KC +++VFDLS
Sbjct: 663 RIGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLS 722

Query: 725 LARGLDYYTGVIYEAIFKGGTQVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPL 784
           LARGLDYYTGVI+EA+               I   +G                       
Sbjct: 723 LARGLDYYTGVIFEAVC--------------IGAEVGSI--------------------- 782

Query: 785 LQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQTTRA 839
               G+ G    L  +F      K V  V   L + R  +     I E + +   Q  R 
Sbjct: 783 ----GAGGRYDNLIGMF----GTKQVPAVGMSLGIERVFN-----IMEELNEKQKQVIRP 842

BLAST of Cp4.1LG01g26330 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-09
Identity = 42/137 (30.66%), Postives = 77/137 (56.20%), Query Frame = 0

Query: 510 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 569
           K++ RK+L  +L+  GVP + F  +C  IDK++K   D+IK+E+    G++ +  E++  
Sbjct: 226 KVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQL-- 285

Query: 570 FVKERGHPLELLA-KLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLAR 629
                   L++L+ K   +   ++   G+  A+++L+ LF   EK    + + FD S+ R
Sbjct: 286 --------LQVLSVKSLDDLEDII--GGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVR 345

Query: 630 GLDYYTGVIYEAIFKGG 646
           GL YYTG+++E   + G
Sbjct: 346 GLAYYTGIVFEGFDRKG 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IYF83.6e-19746.06Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... [more]
P934225.7e-13438.66Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
E9QI363.4e-6234.92Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2[more]
Q2KI844.5e-6234.16Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1[more]
Q610354.5e-6233.13Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023536635.10.082.18histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
KAG7033415.10.080.57Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. argy... [more]
KAG6602728.10.081.75Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022954606.10.081.32histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 hi... [more]
XP_022990844.10.081.32histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GRC10.081.32Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1[more]
A0A6J1JT520.081.32Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1[more]
A0A6J1DGB60.075.00Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1[more]
A0A5A7T4J40.072.82Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A5D3C5F80.072.82Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
Match NameE-valueIdentityDescription
AT3G02760.12.6e-19846.06Class II aaRS and biotin synthetases superfamily protein [more]
AT3G46100.11.2e-0930.66Histidyl-tRNA synthetase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 433..508
e-value: 3.5E-20
score: 74.1
coord: 509..647
e-value: 1.1E-41
score: 144.8
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 648..745
e-value: 4.7E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR11476HISTIDYL-TRNA SYNTHETASEcoord: 740..839
coord: 648..737
NoneNo IPR availablePANTHERPTHR11476:SF7HISTIDYL-TRNA SYNTHETASEcoord: 508..646
coord: 648..737
NoneNo IPR availablePANTHERPTHR11476:SF7HISTIDYL-TRNA SYNTHETASEcoord: 131..508
NoneNo IPR availablePANTHERPTHR11476HISTIDYL-TRNA SYNTHETASEcoord: 131..508
NoneNo IPR availablePANTHERPTHR11476:SF7HISTIDYL-TRNA SYNTHETASEcoord: 740..839
NoneNo IPR availablePANTHERPTHR11476HISTIDYL-TRNA SYNTHETASEcoord: 508..646
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 750..838
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 652..739
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 445..646
IPR041715Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domainPFAMPF13393tRNA-synt_Hiscoord: 508..644
e-value: 1.6E-9
score: 37.4
coord: 677..739
e-value: 3.3E-6
score: 26.6
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 746..839
e-value: 1.5E-18
score: 68.4
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 760..836
e-value: 2.5E-10
score: 40.4
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 15..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g26330.1Cp4.1LG01g26330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006427 histidyl-tRNA aminoacylation
biological_process GO:0032543 mitochondrial translation
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004821 histidine-tRNA ligase activity
molecular_function GO:0003824 catalytic activity