Cp4.1LG01g24900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g24900
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionnifU-like protein 4, mitochondrial
LocationCp4.1LG01: 18642510 .. 18646906 (+)
RNA-Seq ExpressionCp4.1LG01g24900
SyntenyCp4.1LG01g24900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGGATTTAGTAACATTTTTTTTAGCCTTTTGCAAAATCAGGGAGAAGGATGAGGGGATTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTCCGGGATTGAATTCTCGAGGCGTTGGGAAGATCCGATTTGTTTCTGCTGCAGCAACTTCTTCTCTATTCCGCGGCGAAGAAGCTGCTTCCGATTCTGTATCATCTAGAACTTTCTCTGATTCTTACTTGCGCTCCTGGAAATGGAACCGATTATTCGGAGGTGCGTCGATTTTGATGAACTAGCAATGGAAACGTTTCGTATTATGTGTATATAATCTGTTGTATTTTGTTGCATTCAGGACAGAGAAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCTAATGCGCGTTCGGCTATGAACTCCCCACTGGCGAGAGCCCTTTATGGTGTTGATGGTACGTAGTTTGAACTTATATTATTATCCTTTTGGGTTTCTGTTGGACTTTCGATTGGGCAAAGCCCTTTTCCTTTTTGTGTGTGCCCTTGTTAAGGATTTGTTAGAATGAATAAGTGAATTGTAGGAATAGAAAAGTTGCATGTGTCATGGTCATGTTTGTTCAAGGCGGGTTACCCATGGTTTGACCATTGTATGCCAAAACCCTTGTCTTGTATCACTTTATTGCTTTCCTTTACTGTTTTCATGATGCGTAGTATTCTTATTGTATTTCTTTAAGTGTATGACGATACGGTAGAATCATGTTTATGCTTGGTAGACCTGGTAAACTTGAAATGCCTCATAATATATTATAGGAGGATACAACTAGTTGTCAATTTTTCCTACCCTGAGTTATATGCAAGTCGTGATGTTTCTTTCTTTGCTTGTAGCCATATAACGTTGTTTTCTTTAGTTCTTTCAAATCTTGCTTTCAAACTCTCTTTCTAAAATCTCTCTCTACCCCCCGTTTTCTAAAAAAATTTTCTCAAAGCGGGCTAGAGATTTGAGTATACGTTGTCAAGTTGAAGGCGACACAATTTCAAGTTGGTTGATTCACTTAGTGGTGAGAGTGAGTGTTGCACAATCGCTTACAAACGTCCCATAAGAGTGCGATTGTGACACGTGGCATAATGAATAAGTGCTACGACTCGAGTTCTAGGTTATGGAGGATTCTTACCAAAAATAACTGTACAATTTAAGTTAACCAACTCTAATTATAATCGTTTCGCTTCATTTTTCCCATATGTAAGGACGTTGTTGAATTGGTAGGTCGAATTTTCATCGGTTACAATGACTTAGTGATGGTTTACAAATATTCTCACTGATCTCATTGATGTCTGGTGTGGGGCTTACTTGTTTGCTTTGCAGGTGTTGTTCGAGTTTTCTTTGGATCAGATTTTGTTACTGTTACAAAGTCAAATGATGCTTCTTGGGATTTCCTGAAGCCCGAAATTTTTGCTGCAATCATGGACTTCTATTCTTCGGGGCAACCACTGTTTCTAGACTCTCAAACTGCTGCTGCCATGGACACAGCTATCAATGAAGTATATCTTCTGAGTTCTTACTGCAAATTAGTGCTCTTCCTTTTTAGATTTTTACTCATTAAGAAACAACTTAATTTTATAGATCACATATTGTTCTCTGAGGATGTTTATGGTAATTTTATATTAGATTGCTTTTTGATTGTGAACTTTGCACTTCTACAATGTTCTTTTTCCTCTCTTCCTCTCTTGCTATCATCTACATCAATCTACATGTAAGAGTCCTTGCCCACCGCTAGCAGATATTGTCCTCTTTAGGCTTTTTCATTCAGACTTTCCTTCGAGGTTTTTAAAATGAGTCTATTAGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAAATGATGTGGGATCTCACAAACCACCTCCTTCGGGGCCCAGCGACCTCACTAGCACTCATTCCCTTCTCCAATCAATGTGGGACCCCTCAATCTACCCCCCATCGGGGCCCAGTGTCTTTGCTGGCACACCGCCTCGTGTCCACCCCCTTCGGTGTCTGGCTTTGATACCATTTGTAACAGCCCAAGTCCACCGCTAGCCGATATTGTCCTCTTTGGGTTTTCCCTTCTGGGCTTCCCTCAAGGTTTTTATAACGTGTCTGGTAGGTAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCCCCTCTCCTACCGACGTGGGATCTCACACTACATAATGTAATACTAGGTTGGATGAGAGTTCCTTGGTGAAGCCATACTAATTTATATAATTATATACGTAGATTTCTAATTGTTCCTTAAATACTAATATAGAAATATGAACATTTAACATCATCACACTTCAGATTAGAAGTGCTTGAGGCTTAGGTATTAATAGCATAATGTGATGCCTTGCAGTTCTATTGAAGACTCCTGCTCCTTATTCTTATTGAACTTACTGTATTGTAATCTTACTCCTTTGGATTCAACTTCAGCTAAGAACTTGTTTTCTATGTCAACGTCTTCTATCATATGTCAGATTTCTTTCGTAATATTATTTTTTTTGAGATTTGACCTCATGGTGTGATTGCTTGCCCTGCTACAAGTATTGAATCTTTCCTTAAAACATATACTTGTCATGTTGGAATTTGAAACATGATATATTCTTATAATTTAGTAATTTGATTCAACTTAGTTCTTAATAGATCCGTCCACATGTGCCTACTTTTTAGGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTCGAGACCCGTATTCGACCAGCAGTACAAGATGATGGCGGAGATATTGAGTACAGGGGATATGATGAGTAGGTTTTGCTGGATTCAGTTTAAGGTGTTCTGTAAAGTATCATATTGCTTATATCGATTGAACTTTGCATTTCTGTTTTCTTCAGAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGCGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAGGGTTATATGATCTTTTTATATTTGTAGTTACCTCACATTCAATGCAAAGCATATCCAATTTTCCGCTTGCAGCATTTCTGGCACAATTGCTCACGCATATTGATTGCTTTTGTTTGCTGATATTTATGCTGGCAGTATCTTCTATATGAATACCCACATTTCTTATATACATTTAGTTGCATGATGGAAGTATTGAGGTTGAATGCACACCAACTGGAATGGAAGGGCTTATTCTTTTTAGATAATAGGAGTGGAAGAACATTGCATCGGTATAATTTAGATTGAATCTTATTGTCATGACTAGAAAGTTGGTTTTCCAACTTCTTTATCCCTCCATAATATAGTCTTCTTCTCCAACGCACTATTACATGTTACATGTTGTAACAGTCTAAGCCCACTGATAGCAGATATTGTCCACTTTAGCCCGTTATGTATCGTTATTAACCTCACGGTTTTAAAACGTGTCTACCAGGGAGAGGTTTTTACACCCTTATAAGGGATGTTTCATTCCCCTCTCCAACCGACGTGGGATCTCACAATCCACCCCCCGGGTTCAGAGTCCTCATTGGCACACCGCTCGGTGTCTAGCTCTGATACCACTTGTAACAGCCCAAGCCCACCGCTAGCAGATATTATCCGGTTTTAAAACGTGTCTATTAGGGAGATGTTTCCACACCCTTATAAAAAAAGGTTTTGCGTGAGATCTCACAACTACTGTTCATGTTTTACTATCATATGGAATGCAGTATGATCTTATTGGATAACAAGAATTCATCGACAAACGGAGCATGTTTGATAGGAAAAAGGTATTGTCCCCAAACACAGCCAAGTTAGAGAATCACTTTAAGAGGAGATTTAGAAATAGAATGATGTTTCAAAGCTGCGTAGCCCAATCCTAATGTATAGATCGTTTCCTCTTTTGTTCTTATGCTTCTCAGAATTATAGTATCTTTTTCTTGGATTATCTATGATTTAAATGTCTATTCTGGATTTATAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGACGATGTAAAGTCAACCAGCCAGATGGAGTAAAAGGCGAGCTGGTCGATTCGTTCTTTGTTTCGTTGACCCACAACACGATCTCGAGGGTAACCCGTACGAGTTGGTCGATTCCTTCTTCGTTGGGTCGAGCTACAAGCCTACAAGCTAAAAAACCATAGTTTATAGAGTATAGTGTTACTTTTATTCCTCGCTAAGAAAAAAAATCTGTGAATATTGAACCGTAACTAGGATTTCATGGACTTTATTTTGGACTTCCGAAAATTTAAGGTTTTATTAAACATAATTTTTAATT

mRNA sequence

GCGGATTTAGTAACATTTTTTTTAGCCTTTTGCAAAATCAGGGAGAAGGATGAGGGGATTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTCCGGGATTGAATTCTCGAGGCGTTGGGAAGATCCGATTTGTTTCTGCTGCAGCAACTTCTTCTCTATTCCGCGGCGAAGAAGCTGCTTCCGATTCTGTATCATCTAGAACTTTCTCTGATTCTTACTTGCGCTCCTGGAAATGGAACCGATTATTCGGAGGACAGAGAAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCTAATGCGCGTTCGGCTATGAACTCCCCACTGGCGAGAGCCCTTTATGGTGTTGATGGTGTTGTTCGAGTTTTCTTTGGATCAGATTTTGTTACTGTTACAAAGTCAAATGATGCTTCTTGGGATTTCCTGAAGCCCGAAATTTTTGCTGCAATCATGGACTTCTATTCTTCGGGGCAACCACTGTTTCTAGACTCTCAAACTGCTGCTGCCATGGACACAGCTATCAATGAAGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTCGAGACCCGTATTCGACCAGCAGTACAAGATGATGGCGGAGATATTGAGTACAGGGGATATGATGAAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGCGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGACGATGTAAAGTCAACCAGCCAGATGGAGTAAAAGGCGAGCTGGTCGATTCGTTCTTTGTTTCGTTGACCCACAACACGATCTCGAGGGTAACCCGTACGAGTTGGTCGATTCCTTCTTCGTTGGGTCGAGCTACAAGCCTACAAGCTAAAAAACCATAGTTTATAGAGTATAGTGTTACTTTTATTCCTCGCTAAGAAAAAAAATCTGTGAATATTGAACCGTAACTAGGATTTCATGGACTTTATTTTGGACTTCCGAAAATTTAAGGTTTTATTAAACATAATTTTTAATT

Coding sequence (CDS)

ATGAGGGGATTAGGAAGTTTGATTGTGCGAGCTCGAAGAATTCCGGGATTGAATTCTCGAGGCGTTGGGAAGATCCGATTTGTTTCTGCTGCAGCAACTTCTTCTCTATTCCGCGGCGAAGAAGCTGCTTCCGATTCTGTATCATCTAGAACTTTCTCTGATTCTTACTTGCGCTCCTGGAAATGGAACCGATTATTCGGAGGACAGAGAAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCTAATGCGCGTTCGGCTATGAACTCCCCACTGGCGAGAGCCCTTTATGGTGTTGATGGTGTTGTTCGAGTTTTCTTTGGATCAGATTTTGTTACTGTTACAAAGTCAAATGATGCTTCTTGGGATTTCCTGAAGCCCGAAATTTTTGCTGCAATCATGGACTTCTATTCTTCGGGGCAACCACTGTTTCTAGACTCTCAAACTGCTGCTGCCATGGACACAGCTATCAATGAAGATGATTCTGAAACTGTTGCTATGATTAAAGAGCTGCTCGAGACCCGTATTCGACCAGCAGTACAAGATGATGGCGGAGATATTGAGTACAGGGGATATGATGAAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGCGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGACGATGTAAAGTCAACCAGCCAGATGGAGTAA

Protein sequence

MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME
Homology
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BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match: Q9LIG6 (NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 6.0e-101
Identity = 197/283 (69.61%), Postives = 229/283 (80.92%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEE----AASDSVSSRTFS--- 60
           M+G+  L+    RI G   + V     V+++++SSL         +A++ ++SRT     
Sbjct: 1   MKGIARLVTSLSRIGG--RKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ---DSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMN 120
              +S L + KWN L GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ 
Sbjct: 61  PSLNSSLLAQKWNFL-GGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALG 120

Query: 121 SPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQT 180
           SPLA+++Y +DGVVRVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDSQ 
Sbjct: 121 SPLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQA 180

Query: 181 AAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSG 240
           AAA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D E+GIVKLRMQGACSG
Sbjct: 181 AAAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSG 240

Query: 241 CPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQM 274
           CPSSSVTLKSGIENMLMHYV EVKGVEQEFD E+++   + +M
Sbjct: 241 CPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGTLSGEM 280

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match: Q9C8J2 (NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.9e-94
Identity = 177/243 (72.84%), Postives = 203/243 (83.54%), Query Frame = 0 

Query: 27  FVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSL 86
           F+SAA    +   E   +++  + + S S+  +W        QRRTMFI+TQSTPNPSSL
Sbjct: 35  FISAATHFPVNFSESITTNASRNCSRSSSFPFNWI------EQRRTMFIQTQSTPNPSSL 94

Query: 87  MFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFL 146
           MF PGKPVME+GSADFPN+RSAM+SPLA+A++ +DGVVRVF+GSDFVTVTKS+D +WD L
Sbjct: 95  MFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDIL 154

Query: 147 KPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGG 206
           KP+IFA +MDFYSSGQPLFLDSQ  AA DTAI+EDDSETVAMIKELLETRIRP+VQDDGG
Sbjct: 155 KPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGG 214

Query: 207 DIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDD 266
           DIEY G+D ETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EE+ 
Sbjct: 215 DIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEG 271

Query: 267 VKS 270
             S
Sbjct: 275 TSS 271

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match: Q8SY96 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG32500 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.1e-61
Identity = 119/201 (59.20%), Postives = 150/201 (74.63%), Query Frame = 0 

Query: 70  RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLARALYGVDGVVRVFF 129
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A NSPLA+ L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHNSPLAKLLFRVEGVKGVFF 123

Query: 130 GSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAM 189
           G+DFVT++K   A W  +KPE+FA IMDF++SG P+  D+Q  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 190 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 249
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EGGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 250 YVPEVKGVEQEFDVEEDDVKS 270
           Y+PEV+ VEQ FD  +  ++S
Sbjct: 244 YIPEVESVEQVFDEADRMIES 260

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match: B3NYF7 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX=7220 GN=GG17526 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-60
Identity = 117/193 (60.62%), Postives = 146/193 (75.65%), Query Frame = 0 

Query: 70  RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLARALYGVDGVVRVFF 129
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A +SPLA+ L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 130 GSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAM 189
           G+DFVT++K   A W  +KPE+FA IMDF++SG P+  D+Q  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 190 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 249
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 250 YVPEVKGVEQEFD 262
           Y+PEV+ VEQ FD
Sbjct: 244 YIPEVESVEQVFD 252

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match: B4IMF6 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila sechellia OX=7238 GN=GM13534 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-60
Identity = 116/193 (60.10%), Postives = 146/193 (75.65%), Query Frame = 0 

Query: 70  RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLARALYGVDGVVRVFF 129
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A +SPLA+ L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 130 GSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAM 189
           G+DF+T++K   A W  +KPE+FA IMDF++SG P+  D+Q  A  DT I EDD ETV M
Sbjct: 124 GADFITISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 190 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 249
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 250 YVPEVKGVEQEFD 262
           Y+PEV+ VEQ FD
Sbjct: 244 YIPEVESVEQVFD 252

[Close]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match: XP_023517879.1 (nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536 bits (1381), Expect = 5.84e-192
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274

[Close]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match: XP_022960817.1 (nifU-like protein 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 529 bits (1363), Expect = 3.12e-189
Identity = 272/274 (99.27%), Postives = 273/274 (99.64%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNR FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNR-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQEFDVEEDD+KSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEDDIKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match: XP_022990438.1 (nifU-like protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 517 bits (1332), Expect = 1.66e-184
Identity = 267/274 (97.45%), Postives = 268/274 (97.81%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLN RGVGKIRFVSAAATSSLFRGEEAASDSVSSRT SDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNR FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNR-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTV KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIV LRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQ FD+EEDDVKSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQVFDIEEDDVKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match: KAG7032942.1 (NifU-like protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501 bits (1289), Expect = 3.19e-178
Identity = 254/256 (99.22%), Postives = 255/256 (99.61%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGV 256
           GIENMLMHYVPEV+ V
Sbjct: 241 GIENMLMHYVPEVRVV 256

[Close]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match: KAG6602261.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491 bits (1265), Expect = 2.09e-174
Identity = 251/252 (99.60%), Postives = 251/252 (99.60%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNR FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNR-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPE 252
           GIENMLMHYVPE
Sbjct: 241 GIENMLMHYVPE 251

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match: A0A6J1H8H2 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505 PE=3 SV=1)

HSP 1 Score: 529 bits (1363), Expect = 1.51e-189
Identity = 272/274 (99.27%), Postives = 273/274 (99.64%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNR FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNR-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQEFDVEEDD+KSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEDDIKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match: A0A6J1JTA1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 PE=3 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 8.03e-185
Identity = 267/274 (97.45%), Postives = 268/274 (97.81%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRIPGLN RGVGKIRFVSAAATSSLFRGEEAASDSVSSRT SDSYLRSW
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWNR FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV
Sbjct: 61  KWNR-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTV KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIV LRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQ FD+EEDDVKSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQVFDIEEDDVKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match: A0A6J1FIB0 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 1.98e-169
Identity = 246/274 (89.78%), Postives = 258/274 (94.16%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRI  L S GVGKIRF SAA TSS+F G EAAS SVSSRT  D  LRSW
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSRTCFDPLLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           +W++ FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGV
Sbjct: 61  RWSQ-FGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGV+RVFFGSDFVTVTKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINE
Sbjct: 121 DGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQEFDVEE+DVKSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match: A0A6J1JQB1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 PE=3 SV=1)

HSP 1 Score: 478 bits (1230), Expect = 2.82e-169
Identity = 245/274 (89.42%), Postives = 259/274 (94.53%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVRARRI  L S G GKIRF SAA TSS+F G+EAAS SVSSRT SD +LRSW
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
            W++ FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNAR+AMNSPLA+ALYGV
Sbjct: 61  SWSQ-FGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGV 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGV+RVFFGSDFVTVTKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINE
Sbjct: 121 DGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQME 274
           GIENMLMHYVPEVKGVEQEFDVEE+DVKSTSQME
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEEDVKSTSQME 273

[Close]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match: A0A6J1BW08 (nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006227 PE=3 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 1.15e-165
Identity = 239/271 (88.19%), Postives = 255/271 (94.10%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60
           MRGLGSLIVR RRIP ++SRG GKIR +S A TSSLF GE+AAS S +SR+ S+SYLRSW
Sbjct: 1   MRGLGSLIVRVRRIPRMDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSW 60

Query: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120
           KWN+ FGGQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG+
Sbjct: 61  KWNQ-FGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGI 120

Query: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180
           DGVVRVFFGSDFVTVTKS DA+WDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINE 180

Query: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240
           DDSE VAMIKELLETRIRPAVQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKS 240

Query: 241 GIENMLMHYVPEVKGVEQEFDVEEDDVKSTS 271
           GIENMLMHYVPEVKGVEQEFDVEE+D  STS
Sbjct: 241 GIENMLMHYVPEVKGVEQEFDVEEEDAISTS 270

[Close]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 368.6 bits (945), Expect = 4.3e-102
Identity = 197/283 (69.61%), Postives = 229/283 (80.92%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEE----AASDSVSSRTFS--- 60
           M+G+  L+    RI G   + V     V+++++SSL         +A++ ++SRT     
Sbjct: 1   MKGIARLVTSLSRIGG--RKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ---DSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMN 120
              +S L + KWN L GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ 
Sbjct: 61  PSLNSSLLAQKWNFL-GGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALG 120

Query: 121 SPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQT 180
           SPLA+++Y +DGVVRVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDSQ 
Sbjct: 121 SPLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQA 180

Query: 181 AAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSG 240
           AAA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D E+GIVKLRMQGACSG
Sbjct: 181 AAAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSG 240

Query: 241 CPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDDVKSTSQM 274
           CPSSSVTLKSGIENMLMHYV EVKGVEQEFD E+++   + +M
Sbjct: 241 CPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEDEEGTLSGEM 280

[Close]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match: AT1G51390.1 (NFU domain protein 5 )

HSP 1 Score: 347.1 bits (889), Expect = 1.3e-95
Identity = 177/243 (72.84%), Postives = 203/243 (83.54%), Query Frame = 0 

Query: 27  FVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSL 86
           F+SAA    +   E   +++  + + S S+  +W        QRRTMFI+TQSTPNPSSL
Sbjct: 35  FISAATHFPVNFSESITTNASRNCSRSSSFPFNWI------EQRRTMFIQTQSTPNPSSL 94

Query: 87  MFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFL 146
           MF PGKPVME+GSADFPN+RSAM+SPLA+A++ +DGVVRVF+GSDFVTVTKS+D +WD L
Sbjct: 95  MFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDIL 154

Query: 147 KPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGG 206
           KP+IFA +MDFYSSGQPLFLDSQ  AA DTAI+EDDSETVAMIKELLETRIRP+VQDDGG
Sbjct: 155 KPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGG 214

Query: 207 DIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEDD 266
           DIEY G+D ETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EE+ 
Sbjct: 215 DIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEG 271

Query: 267 VKS 270
             S
Sbjct: 275 TSS 271

[Close]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match: AT3G20970.2 (NFU domain protein 4 )

HSP 1 Score: 279.3 bits (713), Expect = 3.4e-75
Identity = 151/224 (67.41%), Postives = 177/224 (79.02%), Query Frame = 0 

Query: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEE----AASDSVSSRTFS--- 60
           M+G+  L+    RI G   + V     V+++++SSL         +A++ ++SRT     
Sbjct: 1   MKGIARLVTSLSRIGG--RKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 61  ---DSYLRSWKWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMN 120
              +S L + KWN L GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ 
Sbjct: 61  PSLNSSLLAQKWNFL-GGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALG 120

Query: 121 SPLARALYGVDGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQT 180
           SPLA+++Y +DGVVRVFFGSDFVTVTKS+D SWD LKPEIFAA+MDFYSSGQPLFLDSQ 
Sbjct: 121 SPLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQA 180

Query: 181 AAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYD 215
           AAA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D
Sbjct: 181 AAAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFD 221

[Close]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 62.8 bits (151), Expect = 5.0e-10
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 0 

Query: 158 YSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLET---RIRPAVQDDGGDIEYRGYD 217
           + S + LF  S+   A+ T    D    V + +E +E+    IRP +  DGG++    ++
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 218 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 264
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

[Close]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 62.8 bits (151), Expect = 5.0e-10
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 0 

Query: 158 YSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLET---RIRPAVQDDGGDIEYRGYD 217
           + S + LF  S+   A+ T    D    V + +E +E+    IRP +  DGG++    ++
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 218 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 264
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g24900 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LIG66.0e-10169.61NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2... [more]
Q9C8J21.9e-9472.84NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2... [more]
Q8SY966.1e-6159.20NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogas... [more]
B3NYF71.0e-6060.62NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX... [more]
B4IMF61.4e-6060.10NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila sechellia... [more]
BLAST of Cp4.1LG01g24900 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023517879.15.84e-192100.00nifU-like protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022960817.13.12e-18999.27nifU-like protein 4, mitochondrial [Cucurbita moschata][more]
XP_022990438.11.66e-18497.45nifU-like protein 4, mitochondrial [Cucurbita maxima][more]
KAG7032942.13.19e-17899.22NifU-like protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602261.12.09e-17499.60NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. soror... [more]
BLAST of Cp4.1LG01g24900 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H8H21.51e-18999.27nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505... [more]
A0A6J1JTA18.03e-18597.45nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 P... [more]
A0A6J1FIB01.98e-16989.78nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668... [more]
A0A6J1JQB12.82e-16989.42nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 P... [more]
A0A6J1BW081.15e-16588.19nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11100622... [more]
BLAST of Cp4.1LG01g24900 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G20970.14.3e-10269.61NFU domain protein 4 [more]
AT1G51390.11.3e-9572.84NFU domain protein 5 [more]
AT3G20970.23.4e-7567.41NFU domain protein 4 [more]
AT5G49940.15.0e-1035.78NIFU-like protein 2 [more]
AT5G49940.25.0e-1035.78NIFU-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014824Scaffold protein Nfu/NifU, N-terminalSMARTSM00932Nfu_N_3acoord: 75..161
e-value: 8.5E-45
score: 164.8
IPR014824Scaffold protein Nfu/NifU, N-terminalPFAMPF08712Nfu_Ncoord: 75..161
e-value: 5.5E-29
score: 100.1
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilyGENE3D3.30.1370.70coord: 66..167
e-value: 8.2E-36
score: 123.9
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilySUPERFAMILY110836Hypothetical protein SAV1430coord: 73..163
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 183..258
e-value: 2.9E-34
score: 118.9
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 175..261
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 189..257
e-value: 1.2E-29
score: 102.2
NoneNo IPR availablePANTHERPTHR11178:SF43NIFU-LIKE PROTEIN 5, MITOCHONDRIALcoord: 27..269
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 27..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g24900.1Cp4.1LG01g24900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding