Cp4.1LG01g23440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g23440
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	monodehydroascorbate reductase 4, peroxisomal
Location	Cp4.1LG01: 19658511 .. 19662258 (+)
RNA-Seq Expression	Cp4.1LG01g23440
Synteny	Cp4.1LG01g23440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCTTCCAAGGGAGGGCAGGGCTATGTTGTCCAGAATCGGCTGCACCTCCGAAGACCAATAACGAAAAAGAGAGGGAGAGATGGGAAGGGCGTTCGTGTATGTGATTCTTGGTGGAGGTGTAGCATCTGGATATGCAGCCCTTGAATTTACGAAGAGGGGGGTCTCTCATGGCGAACTCTGCATAATTTCCGAGGAAACTGTCAGCAATTCCTTTCCTCCATCTCTCTCTCTATTTCCTTTTATTGCTTACAATGTTTCATTTAAGAATTATCGCGTTTCTTAGGGACGCACTTACGAGATTCCACTTTTAGTTCAAAATAGGAGTCGACTTTTCTCAACTCAATTCACCCATTCAGCCTTCCTTTTGTAAATGGAAAGATTTATTTAATCGTTTTTGTTCTTATACTGTTTTGTATGTATGTATGTATTTGTCATCAACTGGAGATATAGTAAATTCCATTAACTTGATTGAAATTTTATAGCCTAATATGGAACTAACAGATTACAGATTTGACTGTTTTTGGTACTCGTAATTCTTCCGTATGATTTGAATATAATTAATTACCATATATGACACGGAATTTCTCCGCTGAGTGCTGATTCCCTGTTGTAGTGGAGCATCTGAGTTGGATTAATCATGTAACTAGAGGATAAATTGGTGTTTTTCTTTTCTTTTTATAAAAGCAGTAAGGTTATATTAAAAGATGCATGTTTGTTGTTTTCTTTCATCTGGTTGTTATGTGTCAAGTGGGAATTATCTTTCATTTTTTATGACACAGTTTCTTTGAACCTCTGATAGGTTGCACCTTATGAGAGGCCTGCTTTAAGTAAAGGGTATTTGCTTCCAGAAGGTAGGACTTCTAATCTTGCTGTATCACTGGTTATTTATGTATCATAGGGTAGAAAAGAAAATAGAACAAACGTCTTCAGTTTGAGACGACTAAGGCCTTTAAGGATACATTTCTTCTAGAATTGTTGAAAACAAGACGTTTCCTCAGGCATAGGCCAGTTGCTCTGTCTAGCTAGCCTTGTTCTATCTATCATTCAGCAGGATGCTAGTTTTTGTATTCCCTTTTGCCAGTGTTTTTGCTTTTTGGTTTGTTGCTCTATAGGACACATTTGTAATGTGGAATTTTATGCTAATTTGCAGCTCCAGCACGCCTTCCAGCATTCCACACATGTGTTGGCGCTAATCAGGAACGGTTAACGCCAAAATGGTACAAAGAACATGGCAAGTAATTCGTATCTACCTTGCTAGATCTGCAATTCTAATATAAGTAGATGAAGAAATATTACGAACCATTTGAGCATGCTAATCATTTTTTACTTCTGTTCACCTTTTATTGAGTCATTTCATATCTATTTATACCTTTTACCCTTTGTATATTGAAATGCACACGACTTTAGAACCAGAAATCAAAGGAGAATACGATTGTTTAGATCATTTGTTCTCCTTTCTGCCTTTTCATGTAGACATTAGTATATTTCTTGATTCAGGCAGATATAAGTTGTACAGCCTGGTACCAATTCTCTTTTTGTAAGATGTTGTATGGTTCTGTCTTCTTTTCAGGAATTGAATTAGTTCTTGGGACCCGAGTTATCTCAGCTGATGTTAGGCGTAGGACCTTGTTAACAGCAGCTGGAGAAACTATTAGTTACAAGATTCTCATCGTTGCAACAGGTGCTCGGGTATAGAACCAATCTTTTAACGATAACACAAATTTCCTTGTATTCACCTTTAACATTTGCTGATAGTCTAATTTTTTCCCTTTGTTCAGTTAATATCTGAGTTTTAAGTGGTCAAAACTTGTTAAGCATCATGGAGACGCATTTCACCAATTAAATAGATCTCAAAGTTCATGGTAGTCTAGTAATATTCATAGAATCATAGTTCTTGGCTCAAGTCCTTTTTTTTCTTTTTTAACTTGAGTGTAGGCTTTGAAGCTAGAAGAATTTGGAGTGAATGGATCAGATGCAGAAAATGTATGTTATCTACGAAACCTAACGGATGCAAATAGGCTTGTCGATGCGATGAATTCGTGTTCACGGGGTAATGCTGTTATAATTGGTGGTGGCTACATAGGAATGGAGTGTGCTGCATCTCTAGTGATTAACAAAATTAATGTAACAATGGTTTTCCCTGAAGCACAGTGCAGTAAGTAACTTAATTATGATGCTTTCTTTTATTACTTAGTCATTTAACCCTTCTTGATGTATGCACTATTGATCTTGCCCTATATTGTACTATTGGGAAATGAATAGATTATTCCTAAGCTTATGGATCTTTTTGTCTTTATAGTGCCTCGTCTATTTACCCCCAAGATTGCAAGCTTTTATGAAGATTATTACAAGGGTAAAGGAGTTGAGTTTGTCAAAGGAACTGTCTTGACATCATTTGATATGGATTCTAATGGAAAGGTACTACAGACGCTTCTTTAGTGATTAGGATATAATGAGGTGAAACATTATGCATTTGATGAGAGGAAGAGGAACCAATCCCAATGAAAATTTTGTAGCATTCTTTGATCCTGGATTCCATAGAATTGTTTGTTTCTTGAAGTTTTTCCTGAAGATTCCTTGAGAATGAATGACAGACAGGCTAGACACTGTGAATTCAGAATACTGATTTCGGTGTAGTTGTAGATTTGACTGATTATCTAACCATTTTCAGGTTGTTGCTGTCAACCTAAGAGATGGAAAACGGCTACCTGTAGACATGGTTGTTGTTGGAATAGGAATTCGCCCAAACACAAGCCTCTTTGATGGTCAGCTGACCATGGAAAAGGGCGGAATCAAGGTAAACGGAAAGATGCAGTCGAGTAACAGCTCGGTGTATGCAGTTGGAGATGTAGCAGCATTTCCAGTCAAGCTTTTCAGTGAAACCCGGAGACTTGAGCATGTCGACTCTGCCAGAAAATCTGCTAGACATGCGGTCTCTGCCATTATGGACCCAGAAAAAACCGGTGACTTTGACGATGTACCATTTTTCTACTCCAGGGTATTCACACTCTCGTGGCAATTTTACGGGGACAACATTGGGGAGGCTGTACATTTTGGGGATTTCTCAGGGAACACATTTGGAGCATATTGGGTAAACAAAGGTCACCTAGTTGGATCTTTTCTTGAAGGTGGAACAAAAGAAGAGTATGAAGCTATAGCGATGATCACCAGTCTGAAACCTGCAATTGAGGATTTGGCCAAGTTAGAAAGCCAAGGACTGAGCTATGCTATGGCGGTTAGTCGGGAACCACCTCCTCCATCACAAGTCATTAACGTTGGTAGTTCTTGCTCTGGCTCTGGTTCTGGTTCTGGTCCTTACCCTGTTCTAGAAACACCAATGTATAGATGGCATGCGACTGCCGGGGTTATCCTTGCTGGATCGATTGCTGCATTTGCATATTGGTACGGGAGGAGACGTCGCCGATGGTAAGAAGACTCGTTTACCAAGTTGAGGAAGCATCAAGCATTAAAGTTGTGATGATATACCAATCTTGTTCTTGGTATCATTCATAATAGTAGGGAATATTGAATGTTATATTCAATTATAGAAATAGTAAGTTATAATTTACTTGACGGTGTATTCATTTATTCCATGAAGAGTTTCACACACTTTAAGATGATTCAGTTTCATAGTTTTTTAAGTCGCATCTTCTCTCATGTGTGAACGAACTTTTCCTCTTAACTTGAAACAGGGAGTTCCATTACTTGTTGGTTCTAATAATTGAAGACTACTTTTGATGCGATTAT

mRNA sequence

TGCTTCCAAGGGAGGGCAGGGCTATGTTGTCCAGAATCGGCTGCACCTCCGAAGACCAATAACGAAAAAGAGAGGGAGAGATGGGAAGGGCGTTCGTGTGTAGCATCTGGATATGCAGCCCTTGAATTTACGAAGAGGGGGGTCTCTCATGGCGAACTCTGCATAATTTCCGAGGAAACTCTCCAGCACGCCTTCCAGCATTCCACACATGTGTTGGCGCTAATCAGGAACGGAATTGAATTAGTTCTTGGGACCCGAGTTATCTCAGCTGATGTTAGGCGTAGGACCTTGTTAACAGCAGCTGGAGAAACTATTAGTTACAAGATTCTCATCGTTGCAACAGGTGCTCGGGCTTTGAAGCTAGAAGAATTTGGAGTGAATGGATCAGATGCAGAAAATGTATGTTATCTACGAAACCTAACGGATGCAAATAGGCTTGTCGATGCGATGAATTCGTGTTCACGGGGTAATGCTGTTATAATTGGTGGTGGCTACATAGGAATGGAGTGTGCTGCATCTCTAGTGATTAACAAAATTAATGTAACAATGGTTTTCCCTGAAGCACAGTGCATGCCTCGTCTATTTACCCCCAAGATTGCAAGCTTTTATGAAGATTATTACAAGGGTAAAGGAGTTGAGTTTGTCAAAGGAACTGTCTTGACATCATTTGATATGGATTCTAATGGAAAGGTTGTTGCTGTCAACCTAAGAGATGGAAAACGGCTACCTGTAGACATGGTTGTTGTTGGAATAGGAATTCGCCCAAACACAAGCCTCTTTGATGGTCAGCTGACCATGGAAAAGGGCGGAATCAAGGTAAACGGAAAGATGCAGTCGAGTAACAGCTCGGTGTATGCAGTTGGAGATGTAGCAGCATTTCCAGTCAAGCTTTTCAGTGAAACCCGGAGACTTGAGCATGTCGACTCTGCCAGAAAATCTGCTAGACATGCGGTCTCTGCCATTATGGACCCAGAAAAAACCGGTGACTTTGACGATGTACCATTTTTCTACTCCAGGGTATTCACACTCTCGTGGCAATTTTACGGGGACAACATTGGGGAGGCTGTACATTTTGGGGATTTCTCAGGGAACACATTTGGAGCATATTGGGTAAACAAAGGTCACCTAGTTGGATCTTTTCTTGAAGGTGGAACAAAAGAAGAGTATGAAGCTATAGCGATGATCACCAGTCTGAAACCTGCAATTGAGGATTTGGCCAAGTTAGAAAGCCAAGGACTGAGCTATGCTATGGCGGTTAGTCGGGAACCACCTCCTCCATCACAAGTCATTAACGTTGGTAGTTCTTGCTCTGGCTCTGGTTCTGGTTCTGGTCCTTACCCTGTTCTAGAAACACCAATGTATAGATGGCATGCGACTGCCGGGGTTATCCTTGCTGGATCGATTGCTGCATTTGCATATTGGTACGGGAGGAGACGTCGCCGATGGTAAGAAGACTCGTTTACCAAGTTGAGGAAGCATCAAGCATTAAAGTTGTGATGATATACCAATCTTGTTCTTGGTATCATTCATAATAGTAGGGAATATTGAATGTTATATTCAATTATAGAAATAGTAAGTTATAATTTACTTGACGGTGTATTCATTTATTCCATGAAGAGTTTCACACACTTTAAGATGATTCAGTTTCATAGTTTTTTAAGTCGCATCTTCTCTCATGTGTGAACGAACTTTTCCTCTTAACTTGAAACAGGGAGTTCCATTACTTGTTGGTTCTAATAATTGAAGACTACTTTTGATGCGATTAT

Coding sequence (CDS)

TGCTTCCAAGGGAGGGCAGGGCTATGTTGTCCAGAATCGGCTGCACCTCCGAAGACCAATAACGAAAAAGAGAGGGAGAGATGGGAAGGGCGTTCGTGTGTAGCATCTGGATATGCAGCCCTTGAATTTACGAAGAGGGGGGTCTCTCATGGCGAACTCTGCATAATTTCCGAGGAAACTCTCCAGCACGCCTTCCAGCATTCCACACATGTGTTGGCGCTAATCAGGAACGGAATTGAATTAGTTCTTGGGACCCGAGTTATCTCAGCTGATGTTAGGCGTAGGACCTTGTTAACAGCAGCTGGAGAAACTATTAGTTACAAGATTCTCATCGTTGCAACAGGTGCTCGGGCTTTGAAGCTAGAAGAATTTGGAGTGAATGGATCAGATGCAGAAAATGTATGTTATCTACGAAACCTAACGGATGCAAATAGGCTTGTCGATGCGATGAATTCGTGTTCACGGGGTAATGCTGTTATAATTGGTGGTGGCTACATAGGAATGGAGTGTGCTGCATCTCTAGTGATTAACAAAATTAATGTAACAATGGTTTTCCCTGAAGCACAGTGCATGCCTCGTCTATTTACCCCCAAGATTGCAAGCTTTTATGAAGATTATTACAAGGGTAAAGGAGTTGAGTTTGTCAAAGGAACTGTCTTGACATCATTTGATATGGATTCTAATGGAAAGGTTGTTGCTGTCAACCTAAGAGATGGAAAACGGCTACCTGTAGACATGGTTGTTGTTGGAATAGGAATTCGCCCAAACACAAGCCTCTTTGATGGTCAGCTGACCATGGAAAAGGGCGGAATCAAGGTAAACGGAAAGATGCAGTCGAGTAACAGCTCGGTGTATGCAGTTGGAGATGTAGCAGCATTTCCAGTCAAGCTTTTCAGTGAAACCCGGAGACTTGAGCATGTCGACTCTGCCAGAAAATCTGCTAGACATGCGGTCTCTGCCATTATGGACCCAGAAAAAACCGGTGACTTTGACGATGTACCATTTTTCTACTCCAGGGTATTCACACTCTCGTGGCAATTTTACGGGGACAACATTGGGGAGGCTGTACATTTTGGGGATTTCTCAGGGAACACATTTGGAGCATATTGGGTAAACAAAGGTCACCTAGTTGGATCTTTTCTTGAAGGTGGAACAAAAGAAGAGTATGAAGCTATAGCGATGATCACCAGTCTGAAACCTGCAATTGAGGATTTGGCCAAGTTAGAAAGCCAAGGACTGAGCTATGCTATGGCGGTTAGTCGGGAACCACCTCCTCCATCACAAGTCATTAACGTTGGTAGTTCTTGCTCTGGCTCTGGTTCTGGTTCTGGTCCTTACCCTGTTCTAGAAACACCAATGTATAGATGGCATGCGACTGCCGGGGTTATCCTTGCTGGATCGATTGCTGCATTTGCATATTGGTACGGGAGGAGACGTCGCCGATGGTAA

Protein sequence

CFQGRAGLCCPESAAPPKTNNEKERERWEGRSCVASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW

Homology

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot
Match: Q9LK94 (Monodehydroascorbate reductase 4, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=MDAR4 PE=1 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.0e-172
Identity = 321/478 (67.15%), Postives = 366/478 (76.57%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETL----------------QHAFQHSTHVLALIR- 93
           VA+GYAALEFT+RGVS GELCIISEE +                  A   S H       
Sbjct: 14  VAAGYAALEFTRRGVSDGELCIISEEPVAPYERPALSKGFLLPEAPARLPSFHTCVGAND 73

Query: 94  ----------NGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRV S DVRR+TLL++ GETISYK LI+ATGARALKLEEFGV
Sbjct: 74  EKLTPKWYKDHGIELVLGTRVKSVDVRRKTLLSSTGETISYKFLIIATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            GSDAENVCYLR+L DANRL   + S S GNAV+IGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 EGSDAENVCYLRDLADANRLATVIQSSSNGNAVVIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EA CM RLFTPKIAS YEDYY+ KGV+F+KGTVLTSF+ DSN KV AVNL+DG  LP D+
Sbjct: 194 EAHCMARLFTPKIASLYEDYYRAKGVKFIKGTVLTSFEFDSNKKVTAVNLKDGSHLPADL 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLT+EKGGIKVN +MQSS+SSVYA+GDVA FPVKLF E RRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTIEKGGIKVNSRMQSSDSSVYAIGDVATFPVKLFGEMRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDF-SGNT 393
           VDSARKSARHAVSAIMDP KTGDFD +PFFYSRVF  SWQFYGD  G+ VHFG++  G +
Sbjct: 314 VDSARKSARHAVSAIMDPIKTGDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDGKS 373

Query: 394 FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIE-DLAKLESQGLSYAMAVSREPP 453
           FGAYWV KGHLVGSFLEGGTKEEYE I+  T LKPA+  DL +LE +GL +A  V  +  
Sbjct: 374 FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFAHTVVSQQK 433

Query: 454 PPSQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 483
            P +V ++ S+     S S    +++ P+Y WHA  GV++A S+AAFA+WYGRRRRRW
Sbjct: 434 VP-EVKDIPSAEMVKQSAS--VVMIKKPLYVWHAATGVVVAASVAAFAFWYGRRRRRW 488

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot
Match: Q8S3R2 (Monodehydroascorbate reductase 2, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=MDAR2 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 7.2e-158
Identity = 295/480 (61.46%), Postives = 353/480 (73.54%), Query Frame = 0


Query: 34  VASGYAALEFTKR-GVSHGELCIISEETLQ-----------------------HAFQHST 93
           VA+GYAALEF +R G S GELCIISEET+                        H    + 
Sbjct: 14  VAAGYAALEFARRGGYSRGELCIISEETVAPYERPALSKGYLLPEGAARLPGFHTCVGAN 73

Query: 94  HVLALIR----NGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFG 153
             L   +    NGIELVLGT+VI+ADVR +TLLTA GETISYK LI+ATGARALKLEEFG
Sbjct: 74  DELLTAKWYKENGIELVLGTKVITADVRMKTLLTATGETISYKNLIIATGARALKLEEFG 133

Query: 154 VNGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVF 213
           ++GSDA N+CYLRNL DA++LV+ M SC  GNAV+IGGGYIGMECAA+LV N+I VTMVF
Sbjct: 134 ISGSDASNICYLRNLDDADKLVNVMKSCPGGNAVVIGGGYIGMECAAALVTNRIKVTMVF 193

Query: 214 PEAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVD 273
           PE+ CM RLFTPKIA +YE+YY  KGV FVKGTVLTSF+ DS GKV +V L+DGK LP D
Sbjct: 194 PESHCMARLFTPKIAEYYENYYTSKGVTFVKGTVLTSFEKDSTGKVTSVILKDGKHLPAD 253

Query: 274 MVVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSET-RRL 333
           MVVVGIGIR +T LF+GQL ME+GGIKVNG+M +S+ SVYAVGDVAAFP+KLF    RRL
Sbjct: 254 MVVVGIGIRASTGLFEGQLLMEQGGIKVNGQMLTSDGSVYAVGDVAAFPIKLFDGVIRRL 313

Query: 334 EHVDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGN 393
           EHVDSAR++ARHAV+AI++P KT D D +PFFYSRVFTLSWQFYG+N GE VHFGDF+ +
Sbjct: 314 EHVDSARRTARHAVAAILEPSKTKDIDYLPFFYSRVFTLSWQFYGNNTGEVVHFGDFTNS 373

Query: 394 T--FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSRE 453
           +  FGAYWV+K  + G+FLEGG++EEYEAI+ +   K  + ++A+LE QGL +A+  S++
Sbjct: 374 SPRFGAYWVDKSRIRGAFLEGGSREEYEAISNVVRRKAKVINIAELEKQGLMFAIQESQK 433

Query: 454 PPPPSQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 483
             P                  G   + E P Y WHATAGVI A SIAAF YWYGR+RRRW
Sbjct: 434 DLP-----------------DGGLALGEKPTYVWHATAGVIAAASIAAFGYWYGRKRRRW 476

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot
Match: Q8S3R1 (Monodehydroascorbate reductase 1, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=MDAR1 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-135
Identity = 272/482 (56.43%), Postives = 323/482 (67.01%), Query Frame = 0


Query: 34  VASGYAALEFTKR--GVSHGELCIISEETLQ-----------------------HAFQHS 93
           VA+GYAALEF +R  G S  ELCIIS+E                          H    S
Sbjct: 14  VAAGYAALEFVRRNGGASSQELCIISDEHFAPYERPALSKGYLLPQDAPRLPAFHTCVGS 73

Query: 94  THVLA----LIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEF 153
              L        +GI LVLGTRVISADVR++TLLT++GETISYK LIVATGARA+KLEEF
Sbjct: 74  KDELLTEEWYNEHGIVLVLGTRVISADVRQKTLLTSSGETISYKTLIVATGARAVKLEEF 133

Query: 154 GVNGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMV 213
           GV+GSDA NVCYLRN+ DA++LV  M SC  GNAV++GGGYIGMECAA+LV N I VTMV
Sbjct: 134 GVSGSDARNVCYLRNVEDADKLVGVMRSCPGGNAVVVGGGYIGMECAAALVTNNIKVTMV 193

Query: 214 FPEAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPV 273
           FP+  CM RLFTPKIA FYE YY  +GV FVK   +TS  + S GKV AVNL +G+RLP 
Sbjct: 194 FPKKHCMGRLFTPKIAEFYESYYASRGVTFVKEAAVTSMQI-SAGKVTAVNLGNGRRLPA 253

Query: 274 DMVVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLF-SETRR 333
           DMVVVG+G R NT LFDGQL ME GGIKVNG+MQ+S++SVYAVGDVAAFPVKLF  + RR
Sbjct: 254 DMVVVGVGARANTGLFDGQLVMENGGIKVNGRMQASDASVYAVGDVAAFPVKLFGGDVRR 313

Query: 334 LEHVDSARKSARHAVSAIMD-PEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDF- 393
           LEHVD AR++ARHAV+A+++     G  D +PFFYSRVF+LSWQFYGDN GEAVHFGD  
Sbjct: 314 LEHVDCARRTARHAVAAMLEGTGSVGHIDYLPFFYSRVFSLSWQFYGDNAGEAVHFGDLA 373

Query: 394 -------SGNTFGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLS 453
                  +   FGAYWV  G + G+FLEGG+++EYEA+A       A+ D+A+LE +GL+
Sbjct: 374 PPGDGDGAAPKFGAYWVRDGRVAGAFLEGGSRQEYEAVAAAVRRGAAVADVAELERRGLA 433

Query: 454 YAMAVSREPPPPSQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYW 477
           +A                 +  +G G           P   WHAT GV  A SIAAFA W
Sbjct: 434 FA-----------------TQATGGGG---------KPTCAWHATVGVAAAVSIAAFACW 468

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot
Match: Q652L6 (Monodehydroascorbate reductase 3, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=MDAR3 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.6e-104
Identity = 199/420 (47.38%), Postives = 273/420 (65.00%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQ----------HAFQHST------HVLA---- 93
           VA+GYAA EF K+GV  GEL IIS+E +           + F  +       HV      
Sbjct: 16  VAAGYAAREFAKQGVKPGELAIISKEAVAPYERPALSKGYLFPQNAARLPGFHVCVGSGG 75

Query: 94  -------LIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                      GIEL+L T ++ AD+  +TL +A G T +Y+ILI+ATG+  +KL +FG 
Sbjct: 76  ERLLPEWYSEKGIELILSTEIVKADLASKTLTSAVGATFTYEILIIATGSSVIKLSDFGT 135

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            G+D+ N+ YLR + DA++LV A+ +   G AVI+GGGYIG+E +A+L IN  +VTMVFP
Sbjct: 136 QGADSNNILYLREVDDADKLVAAIQAKKGGKAVIVGGGYIGLELSAALKINDFDVTMVFP 195

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           E  CMPRLFT  IA+FYE YY  KGV+ VKGTV   FD D+NG V AVNL++G  L  D+
Sbjct: 196 EPWCMPRLFTADIAAFYESYYTNKGVKIVKGTVAVGFDADANGDVTAVNLKNGSVLEADI 255

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVG+G RP T+LF GQ+  EKGGIK +   ++S   VYAVGDVA FP+K+++E RR+EH
Sbjct: 256 VVVGVGGRPLTTLFKGQVAEEKGGIKTDAFFETSVPGVYAVGDVATFPMKMYNELRRVEH 315

Query: 334 VDSARKSARHAVSAIM---DPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSG 393
           VD ARKSA  AV AI      E   ++D +P+FYSR F L WQFYGDN+G+ + FGD   
Sbjct: 316 VDHARKSAEQAVKAIKGKESGESVVEYDYLPYFYSRSFDLGWQFYGDNVGDTILFGDSDP 375

Query: 394 NT----FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAV 420
            +    FG+YW+  G ++G+FLEGG+ +E +AIA +   +P + ++ +L+ +GL +A  +
Sbjct: 376 TSAKPKFGSYWIKDGKVLGAFLEGGSPDENKAIAKVAKTQPPVANIEELKKEGLQFASKI 435

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot
Match: Q42711 (Monodehydroascorbate reductase, seedling isozyme OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.0e-103
Identity = 199/420 (47.38%), Postives = 278/420 (66.19%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETL----------QHAFQHST------HVLA---- 93
           VA+GYAA EF K+G++ GEL IIS+E +           + F  S       HV      
Sbjct: 15  VAAGYAAREFVKQGLNPGELAIISKEAVAPYERPALSKAYLFPESPARLPGFHVCVGSGG 74

Query: 94  -------LIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                      GIEL+L T ++ AD+  + L +A G+  +Y+ LI+ATG+  +KL +FGV
Sbjct: 75  ERLLPDWYKEKGIELILSTEIVEADLPAKRLRSAHGKIYNYQTLIIATGSTVIKLSDFGV 134

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            G+DA+N+ YLR + DA++LV+A+ +   G  V++GGGYIG+E  A+L IN  +V+MV+P
Sbjct: 135 QGADAKNIFYLREIDDADQLVEAIKAKENGKVVVVGGGYIGLELGAALRINNFDVSMVYP 194

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           E  CMPRLFTP+IA+FYE YY  KG+  +KGTV   F +D+NG+V  V L+DG+ L  D+
Sbjct: 195 EPWCMPRLFTPEIAAFYEGYYAQKGITIIKGTVAVGFTVDTNGEVKEVKLKDGRVLEADI 254

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVG+G RP TSLF GQ+  EKGGIK +   ++S   VYAVGDVA FP+KL++E RR+EH
Sbjct: 255 VVVGVGARPLTSLFKGQIVEEKGGIKTDEFFKTSVPDVYAVGDVATFPLKLYNELRRVEH 314

Query: 334 VDSARKSARHAVSAIMDPEK---TGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSG 393
           VD +RKSA  AV AI   E+     ++D +P+FYSR F LSWQFYGDN+G+AV FGD S 
Sbjct: 315 VDHSRKSAEQAVKAIKASEEGKAIEEYDYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSP 374

Query: 394 NT----FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAV 420
           ++    FG+YW+  G +VG+FLE G+ EE +AIA +  ++P++E    L  +G+S+A  V
Sbjct: 375 DSATHKFGSYWIKDGKVVGAFLESGSPEENKAIAKVARIQPSVESSDLLLKEGISFASKV 434

[Close]

BLAST of Cp4.1LG01g23440 vs. NCBI nr
Match: XP_023515751.1 (monodehydroascorbate reductase 4, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 838 bits (2166), Expect = 1.82e-304
Identity = 433/476 (90.97%), Postives = 438/476 (92.02%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALI------------------ 93
           VASGYAALEFTKRGVSHGELCIISEET+    + +     L+                  
Sbjct: 14  VASGYAALEFTKRGVSHGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGANQ 73

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV
Sbjct: 74  ERLTPKWYKEHGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
           NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM
Sbjct: 194 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH
Sbjct: 254 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 393
           VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF
Sbjct: 314 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 373

Query: 394 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 453
           GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP
Sbjct: 374 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 433

Query: 454 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
           SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 489

[Close]

BLAST of Cp4.1LG01g23440 vs. NCBI nr
Match: XP_022921982.1 (monodehydroascorbate reductase 4, peroxisomal-like isoform X1 [Cucurbita moschata] >KAG6602431.1 Monodehydroascorbate reductase 4, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805 bits (2079), Expect = 2.68e-291
Identity = 420/476 (88.24%), Postives = 429/476 (90.13%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALI------------------ 93
           VASGYAALEFTKRGVSHGELCIISEET+    + +     L+                  
Sbjct: 14  VASGYAALEFTKRGVSHGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGANQ 73

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV
Sbjct: 74  ERLTPKWYKEHGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
           NGSDAENVCYLRNLTDANRLV AMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 NGSDAENVCYLRNLTDANRLVGAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EAQCMPRLFTPKIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DM
Sbjct: 194 EAQCMPRLFTPKIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADM 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 393
           VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTF
Sbjct: 314 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTF 373

Query: 394 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 453
           GAYWVNKGHLVGSFLEGGTKEEYEAIAM+TSLKPAIEDLAKLESQGLSYAMAVSREPPP 
Sbjct: 374 GAYWVNKGHLVGSFLEGGTKEEYEAIAMVTSLKPAIEDLAKLESQGLSYAMAVSREPPP- 433

Query: 454 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
           +QVINVGSS    GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 TQVINVGSS----GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 484

[Close]

BLAST of Cp4.1LG01g23440 vs. NCBI nr
Match: XP_022989902.1 (monodehydroascorbate reductase 4, peroxisomal-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 803 bits (2074), Expect = 1.55e-290
Identity = 420/476 (88.24%), Postives = 428/476 (89.92%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALI------------------ 93
           VASGYAALEFTKRGVSHGELCIISEET+    + +     L+                  
Sbjct: 14  VASGYAALEFTKRGVSHGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGANQ 73

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV
Sbjct: 74  ERLTPKWYKEHGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
           NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EAQCMPRLFTPKIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DM
Sbjct: 194 EAQCMPRLFTPKIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADM 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 393
           VDSARKSARHAVSAIMDPEKTGDFD VPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTF
Sbjct: 314 VDSARKSARHAVSAIMDPEKTGDFDVVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTF 373

Query: 394 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 453
           GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDL KLESQGLSYAMAVSREPPP 
Sbjct: 374 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLTKLESQGLSYAMAVSREPPP- 433

Query: 454 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
           +QVINVGSS    GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 TQVINVGSS----GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 484

[Close]

BLAST of Cp4.1LG01g23440 vs. NCBI nr
Match: XP_022921992.1 (monodehydroascorbate reductase 4, peroxisomal-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 768 bits (1984), Expect = 3.61e-278
Identity = 391/404 (96.78%), Postives = 395/404 (97.77%), Query Frame = 0


Query: 79  IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR 138
           IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR
Sbjct: 4   IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR 63

Query: 139 NLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK 198
           NLTDANRLV AMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK
Sbjct: 64  NLTDANRLVGAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK 123

Query: 199 IASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTS 258
           IASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DMVVVGIGIRPNTS
Sbjct: 124 IASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADMVVVGIGIRPNTS 183

Query: 259 LFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV 318
           LF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV
Sbjct: 184 LFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV 243

Query: 319 SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLVG 378
           SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTFGAYWVNKGHLVG
Sbjct: 244 SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTFGAYWVNKGHLVG 303

Query: 379 SFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCSG 438
           SFLEGGTKEEYEAIAM+TSLKPAIEDLAKLESQGLSYAMAVSREPPP +QVINVGSS   
Sbjct: 304 SFLEGGTKEEYEAIAMVTSLKPAIEDLAKLESQGLSYAMAVSREPPP-TQVINVGSS--- 363

Query: 439 SGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
            GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 364 -GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 402

[Close]

BLAST of Cp4.1LG01g23440 vs. NCBI nr
Match: XP_022989904.1 (monodehydroascorbate reductase 4, peroxisomal-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 767 bits (1980), Expect = 1.47e-277
Identity = 391/405 (96.54%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 78  GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL 137
            IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL
Sbjct: 3   SIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL 62

Query: 138 RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP 197
           RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP
Sbjct: 63  RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP 122

Query: 198 KIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNT 257
           KIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DMVVVGIGIRPNT
Sbjct: 123 KIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADMVVVGIGIRPNT 182

Query: 258 SLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA 317
           SLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA
Sbjct: 183 SLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA 242

Query: 318 VSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLV 377
           VSAIMDPEKTGDFD VPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTFGAYWVNKGHLV
Sbjct: 243 VSAIMDPEKTGDFDVVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTFGAYWVNKGHLV 302

Query: 378 GSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCS 437
           GSFLEGGTKEEYEAIAMITSLKPAIEDL KLESQGLSYAMAVSREPPP +QVINVGSS  
Sbjct: 303 GSFLEGGTKEEYEAIAMITSLKPAIEDLTKLESQGLSYAMAVSREPPP-TQVINVGSS-- 362

Query: 438 GSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
             GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 363 --GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 402

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL
Match: A0A6J1E1V8 (monodehydroascorbate reductase 4, peroxisomal-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430072 PE=4 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 1.30e-291
Identity = 420/476 (88.24%), Postives = 429/476 (90.13%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALI------------------ 93
           VASGYAALEFTKRGVSHGELCIISEET+    + +     L+                  
Sbjct: 14  VASGYAALEFTKRGVSHGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGANQ 73

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV
Sbjct: 74  ERLTPKWYKEHGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
           NGSDAENVCYLRNLTDANRLV AMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 NGSDAENVCYLRNLTDANRLVGAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EAQCMPRLFTPKIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DM
Sbjct: 194 EAQCMPRLFTPKIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADM 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 393
           VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTF
Sbjct: 314 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTF 373

Query: 394 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 453
           GAYWVNKGHLVGSFLEGGTKEEYEAIAM+TSLKPAIEDLAKLESQGLSYAMAVSREPPP 
Sbjct: 374 GAYWVNKGHLVGSFLEGGTKEEYEAIAMVTSLKPAIEDLAKLESQGLSYAMAVSREPPP- 433

Query: 454 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
           +QVINVGSS    GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 TQVINVGSS----GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 484

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL
Match: A0A6J1JH36 (monodehydroascorbate reductase 4, peroxisomal-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486955 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 7.49e-291
Identity = 420/476 (88.24%), Postives = 428/476 (89.92%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALI------------------ 93
           VASGYAALEFTKRGVSHGELCIISEET+    + +     L+                  
Sbjct: 14  VASGYAALEFTKRGVSHGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGANQ 73

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV
Sbjct: 74  ERLTPKWYKEHGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
           NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EAQCMPRLFTPKIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DM
Sbjct: 194 EAQCMPRLFTPKIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADM 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTF 393
           VDSARKSARHAVSAIMDPEKTGDFD VPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTF
Sbjct: 314 VDSARKSARHAVSAIMDPEKTGDFDVVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTF 373

Query: 394 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPP 453
           GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDL KLESQGLSYAMAVSREPPP 
Sbjct: 374 GAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLTKLESQGLSYAMAVSREPPP- 433

Query: 454 SQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
           +QVINVGSS    GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 TQVINVGSS----GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 484

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL
Match: A0A6J1E1W5 (monodehydroascorbate reductase 4, peroxisomal-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430072 PE=4 SV=1)

HSP 1 Score: 768 bits (1984), Expect = 1.75e-278
Identity = 391/404 (96.78%), Postives = 395/404 (97.77%), Query Frame = 0


Query: 79  IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR 138
           IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR
Sbjct: 4   IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR 63

Query: 139 NLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK 198
           NLTDANRLV AMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK
Sbjct: 64  NLTDANRLVGAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPK 123

Query: 199 IASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTS 258
           IASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DMVVVGIGIRPNTS
Sbjct: 124 IASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADMVVVGIGIRPNTS 183

Query: 259 LFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV 318
           LF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV
Sbjct: 184 LFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAV 243

Query: 319 SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLVG 378
           SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTFGAYWVNKGHLVG
Sbjct: 244 SAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTFGAYWVNKGHLVG 303

Query: 379 SFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCSG 438
           SFLEGGTKEEYEAIAM+TSLKPAIEDLAKLESQGLSYAMAVSREPPP +QVINVGSS   
Sbjct: 304 SFLEGGTKEEYEAIAMVTSLKPAIEDLAKLESQGLSYAMAVSREPPP-TQVINVGSS--- 363

Query: 439 SGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
            GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 364 -GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 402

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL
Match: A0A6J1JQN0 (monodehydroascorbate reductase 4, peroxisomal-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486955 PE=4 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 7.11e-278
Identity = 391/405 (96.54%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 78  GIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL 137
            IELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL
Sbjct: 3   SIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYL 62

Query: 138 RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP 197
           RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP
Sbjct: 63  RNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTP 122

Query: 198 KIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNT 257
           KIASFYEDYYK KGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLP DMVVVGIGIRPNT
Sbjct: 123 KIASFYEDYYKAKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPADMVVVGIGIRPNT 182

Query: 258 SLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA 317
           SLF+GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA
Sbjct: 183 SLFEGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHA 242

Query: 318 VSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLV 377
           VSAIMDPEKTGDFD VPFFYSRVFTLSWQFYGDN+GEAVHFGDFSGNTFGAYWVNKGHLV
Sbjct: 243 VSAIMDPEKTGDFDVVPFFYSRVFTLSWQFYGDNVGEAVHFGDFSGNTFGAYWVNKGHLV 302

Query: 378 GSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCS 437
           GSFLEGGTKEEYEAIAMITSLKPAIEDL KLESQGLSYAMAVSREPPP +QVINVGSS  
Sbjct: 303 GSFLEGGTKEEYEAIAMITSLKPAIEDLTKLESQGLSYAMAVSREPPP-TQVINVGSS-- 362

Query: 438 GSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
             GSG GPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 363 --GSGLGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 402

[Close]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL
Match: A0A0A0KUG3 (Pyr_redox_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524740 PE=4 SV=1)

HSP 1 Score: 753 bits (1944), Expect = 2.29e-271
Identity = 390/461 (84.60%), Postives = 411/461 (89.15%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQHAFQHSTHVLALIRN------------GIEL 93
           VA+GYAALEFTKRGVS GELCIISEET+    + +     L+              GIEL
Sbjct: 14  VAAGYAALEFTKRGVSRGELCIISEETVAPYERPALSKGYLLPEAPARLPAFHTCVGIEL 73

Query: 94  VLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLRNLT 153
           VLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLR+L 
Sbjct: 74  VLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGSDAENVCYLRDLN 133

Query: 154 DANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPKIAS 213
           DANR VD M+SCS GNAV+IGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPKIAS
Sbjct: 134 DANRAVDVMHSCSGGNAVVIGGGYIGMECAASLVINKINVTMVFPEAQCMPRLFTPKIAS 193

Query: 214 FYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTSLFD 273
           FYEDYYK KGVEFVKGTVL+SFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTSLF+
Sbjct: 194 FYEDYYKSKGVEFVKGTVLSSFDMDSNGKVVAVNLRDGKRLPVDMVVVGIGIRPNTSLFE 253

Query: 274 GQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDSARKSARHAVSAI 333
           GQLT+EKGGIKVNGKMQSSNSSVYAVGDVAAFPVK+FSETRRLEHVDSARK ARHAV+AI
Sbjct: 254 GQLTLEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKIFSETRRLEHVDSARKCARHAVAAI 313

Query: 334 MDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNTFGAYWVNKGHLVGSFL 393
           M+PEKTGDFD VPFFYSR+F LSWQFYGDNIGE V+FGDFSGNTFGAYWVNKGHLVGSFL
Sbjct: 314 MEPEKTGDFDYVPFFYSRIFALSWQFYGDNIGEVVYFGDFSGNTFGAYWVNKGHLVGSFL 373

Query: 394 EGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAVSREPPPPSQVINVGSSCSGSGS 453
           EGGTKEEY AIAM+TSLKPAIEDLA+LES+GLSYAMA+SREPPPP QV NV  S      
Sbjct: 374 EGGTKEEYAAIAMVTSLKPAIEDLAELESRGLSYAMAISREPPPP-QVTNVDVS------ 433

Query: 454 GSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 482
             GP  V+E PMYRWHATAGVILAGSIAAFAYWYGRRRRRW
Sbjct: 434 --GPSLVIEKPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 465

[Close]

BLAST of Cp4.1LG01g23440 vs. TAIR 10
Match: AT3G27820.1 (monodehydroascorbate reductase 4 )

HSP 1 Score: 607.8 bits (1566), Expect = 7.4e-174
Identity = 321/478 (67.15%), Postives = 366/478 (76.57%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETL----------------QHAFQHSTHVLALIR- 93
           VA+GYAALEFT+RGVS GELCIISEE +                  A   S H       
Sbjct: 14  VAAGYAALEFTRRGVSDGELCIISEEPVAPYERPALSKGFLLPEAPARLPSFHTCVGAND 73

Query: 94  ----------NGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                     +GIELVLGTRV S DVRR+TLL++ GETISYK LI+ATGARALKLEEFGV
Sbjct: 74  EKLTPKWYKDHGIELVLGTRVKSVDVRRKTLLSSTGETISYKFLIIATGARALKLEEFGV 133

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            GSDAENVCYLR+L DANRL   + S S GNAV+IGGGYIGMECAASLVINKINVTMVFP
Sbjct: 134 EGSDAENVCYLRDLADANRLATVIQSSSNGNAVVIGGGYIGMECAASLVINKINVTMVFP 193

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           EA CM RLFTPKIAS YEDYY+ KGV+F+KGTVLTSF+ DSN KV AVNL+DG  LP D+
Sbjct: 194 EAHCMARLFTPKIASLYEDYYRAKGVKFIKGTVLTSFEFDSNKKVTAVNLKDGSHLPADL 253

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           VVVGIGIRPNTSLF+GQLT+EKGGIKVN +MQSS+SSVYA+GDVA FPVKLF E RRLEH
Sbjct: 254 VVVGIGIRPNTSLFEGQLTIEKGGIKVNSRMQSSDSSVYAIGDVATFPVKLFGEMRRLEH 313

Query: 334 VDSARKSARHAVSAIMDPEKTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDF-SGNT 393
           VDSARKSARHAVSAIMDP KTGDFD +PFFYSRVF  SWQFYGD  G+ VHFG++  G +
Sbjct: 314 VDSARKSARHAVSAIMDPIKTGDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDGKS 373

Query: 394 FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIE-DLAKLESQGLSYAMAVSREPP 453
           FGAYWV KGHLVGSFLEGGTKEEYE I+  T LKPA+  DL +LE +GL +A  V  +  
Sbjct: 374 FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFAHTVVSQQK 433

Query: 454 PPSQVINVGSSCSGSGSGSGPYPVLETPMYRWHATAGVILAGSIAAFAYWYGRRRRRW 483
            P +V ++ S+     S S    +++ P+Y WHA  GV++A S+AAFA+WYGRRRRRW
Sbjct: 434 VP-EVKDIPSAEMVKQSAS--VVMIKKPLYVWHAATGVVVAASVAAFAFWYGRRRRRW 488

[Close]

BLAST of Cp4.1LG01g23440 vs. TAIR 10
Match: AT5G03630.1 (Pyridine nucleotide-disulphide oxidoreductase family protein )

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-103
Identity = 199/417 (47.72%), Postives = 270/417 (64.75%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEE--------TLQHAFQH--------STHVLALI-- 93
           VA+GYAA EF  +GV  GEL IIS E         L   + H        + +V A I  
Sbjct: 16  VAAGYAAREFFNQGVKPGELAIISREQVPPYERPALSKGYIHLENKATLPNFYVAAGIGG 75

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                      GIEL+LGT ++ AD+  +TL++  G+   Y+ L+ ATG+  ++L +FGV
Sbjct: 76  ERQFPQWYKEKGIELILGTEIVKADLAAKTLVSGTGQVFKYQTLLAATGSSVIRLSDFGV 135

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            G+DA+N+ YLR L DA+ L  AM +  +G AV++GGGYIG+E  A+L  N ++VTMV+P
Sbjct: 136 PGADAKNIFYLRELEDADYLAYAMETKEKGKAVVVGGGYIGLELGAALKANNLDVTMVYP 195

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           E  CMPRLFT  IASFYE YY  KG+  VKGTV + F  +SNG+V  V L+DG+ L  D+
Sbjct: 196 EPWCMPRLFTAGIASFYEGYYANKGINIVKGTVASGFTTNSNGEVTEVKLKDGRTLEADI 255

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           V+VG+G RP  SLF  Q+  EKGG+K +G  ++S   VYA+GDVA FP+KL++E RR+EH
Sbjct: 256 VIVGVGGRPIISLFKDQVEEEKGGLKTDGFFKTSLPDVYAIGDVATFPMKLYNEMRRVEH 315

Query: 334 VDSARKSARHAVSAIMDPEKTG---DFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSG 393
           VD ARKSA  AV AI   E+     ++D +P+FYSR F LSWQFYGDN+GE+V FGD   
Sbjct: 316 VDHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDP 375

Query: 394 NT----FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYA 417
            +    FG+YW+ +  +VG+FLEGG+ EE  AIA +   +P++E L  L  +GLS+A
Sbjct: 376 ESPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFA 432

[Close]

BLAST of Cp4.1LG01g23440 vs. TAIR 10
Match: AT3G52880.1 (monodehydroascorbate reductase 1 )

HSP 1 Score: 372.1 bits (954), Expect = 6.8e-103
Identity = 191/420 (45.48%), Postives = 274/420 (65.24%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEETLQ----------HAFQHSTHVL----------- 93
           V++GYAA EF  +GV  GEL +IS+E +           + F      L           
Sbjct: 15  VSAGYAAKEFANQGVQPGELAVISKEAVAPYERPALSKGYLFPEGAARLPGFHCCVGSGG 74

Query: 94  ------ALIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                 +  + GIEL+L T ++ AD+  ++L++A G+   Y+ LI+ATG+  L+L +FGV
Sbjct: 75  EKLLPESYKQKGIELILSTEIVKADLSAKSLVSATGDVFKYQTLIIATGSTVLRLTDFGV 134

Query: 154 NGSDAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFP 213
            G+D++N+ YLR + DA++LV+A+ +   G AV++GGGYIG+E +A L IN ++VTMVFP
Sbjct: 135 KGADSKNILYLREIDDADKLVEAIKAKKGGKAVVVGGGYIGLELSAVLRINNLDVTMVFP 194

Query: 214 EAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDM 273
           E  CMPRLFT  IA+FYE YY  KGV+ +KGTV + F    NG+V  V L+DG+ L  D+
Sbjct: 195 EPWCMPRLFTADIAAFYETYYTNKGVKIIKGTVASGFTAQPNGEVKEVQLKDGRTLEADI 254

Query: 274 VVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEH 333
           V+VG+G +P TSLF GQ+  +KGGIK +   ++S   VYAVGDVA FP+K++ + RR+EH
Sbjct: 255 VIVGVGAKPLTSLFKGQVEEDKGGIKTDAFFKTSVPDVYAVGDVATFPLKMYGDVRRVEH 314

Query: 334 VDSARKSARHAVSAIMDPE---KTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSG 393
           VD +RKSA  AV AI   E      ++D +PFFYSR F LSWQFYGDN+G++V FGD + 
Sbjct: 315 VDHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNP 374

Query: 394 NT----FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAV 420
           +     FGAYWV  G +VG+F+EGG+ +E +A+A +   +P+ E L +L  QG+S+A  +
Sbjct: 375 SNPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 434

[Close]

BLAST of Cp4.1LG01g23440 vs. TAIR 10
Match: AT3G52880.2 (monodehydroascorbate reductase 1 )

HSP 1 Score: 369.8 bits (948), Expect = 3.4e-102
Identity = 190/417 (45.56%), Postives = 271/417 (64.99%), Query Frame = 0


Query: 37  GYAALEFTKRGVSHGELCIISEETLQ----------HAFQHSTHVL-------------- 96
           GYAA EF  +GV  GEL +IS+E +           + F      L              
Sbjct: 50  GYAAKEFANQGVQPGELAVISKEAVAPYERPALSKGYLFPEGAARLPGFHCCVGSGGEKL 109

Query: 97  ---ALIRNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGVNGS 156
              +  + GIEL+L T ++ AD+  ++L++A G+   Y+ LI+ATG+  L+L +FGV G+
Sbjct: 110 LPESYKQKGIELILSTEIVKADLSAKSLVSATGDVFKYQTLIIATGSTVLRLTDFGVKGA 169

Query: 157 DAENVCYLRNLTDANRLVDAMNSCSRGNAVIIGGGYIGMECAASLVINKINVTMVFPEAQ 216
           D++N+ YLR + DA++LV+A+ +   G AV++GGGYIG+E +A L IN ++VTMVFPE  
Sbjct: 170 DSKNILYLREIDDADKLVEAIKAKKGGKAVVVGGGYIGLELSAVLRINNLDVTMVFPEPW 229

Query: 217 CMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVDMVVV 276
           CMPRLFT  IA+FYE YY  KGV+ +KGTV + F    NG+V  V L+DG+ L  D+V+V
Sbjct: 230 CMPRLFTADIAAFYETYYTNKGVKIIKGTVASGFTAQPNGEVKEVQLKDGRTLEADIVIV 289

Query: 277 GIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLEHVDS 336
           G+G +P TSLF GQ+  +KGGIK +   ++S   VYAVGDVA FP+K++ + RR+EHVD 
Sbjct: 290 GVGAKPLTSLFKGQVEEDKGGIKTDAFFKTSVPDVYAVGDVATFPLKMYGDVRRVEHVDH 349

Query: 337 ARKSARHAVSAIMDPE---KTGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFSGNT- 396
           +RKSA  AV AI   E      ++D +PFFYSR F LSWQFYGDN+G++V FGD + +  
Sbjct: 350 SRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNP 409

Query: 397 ---FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYAMAV 420
              FGAYWV  G +VG+F+EGG+ +E +A+A +   +P+ E L +L  QG+S+A  +
Sbjct: 410 KPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 466

[Close]

BLAST of Cp4.1LG01g23440 vs. TAIR 10
Match: AT3G09940.1 (monodehydroascorbate reductase )

HSP 1 Score: 349.0 bits (894), Expect = 6.2e-96
Identity = 190/418 (45.45%), Postives = 263/418 (62.92%), Query Frame = 0


Query: 34  VASGYAALEFTKRGVSHGELCIISEE----------TLQHAFQHSTHVLALI-------- 93
           VA GYAA EF+ +G+  GEL IIS+E          T  +        LA I        
Sbjct: 16  VAGGYAAREFSNQGLKPGELAIISKEPVPPFERPELTKVYIDLEVNPTLANIYVCAGTGE 75

Query: 94  ---------RNGIELVLGTRVISADVRRRTLLTAAGETISYKILIVATGARALKLEEFGV 153
                      GI+L++GT ++ AD+  +TL++  G+   Y+ L++ATG+  ++L E GV
Sbjct: 76  AKQYPNWYKEKGIDLIVGTEIVKADLASKTLVSDDGKIYKYQTLLIATGSTNIRLSEIGV 135

Query: 154 NGSDAENVCYLRNLTDANRLVDAMN-SCSRGNAVIIGGGYIGMECAASLVINKINVTMVF 213
             +D +N+ YLR + D++ L  AM     RG AVIIGGG++G+E +++L  N   VTMVF
Sbjct: 136 QEADVKNIFYLREIEDSDELALAMELYVQRGKAVIIGGGFLGLEISSALRANNHEVTMVF 195

Query: 214 PEAQCMPRLFTPKIASFYEDYYKGKGVEFVKGTVLTSFDMDSNGKVVAVNLRDGKRLPVD 273
           PE   + R FT +IASFYE YY  KG++ +KGTV T F  +S+G+V  V L DG+ L  +
Sbjct: 196 PEPWLVHRFFTAEIASFYESYYANKGIKIIKGTVATGFSTNSDGEVTEVKLEDGRTLEAN 255

Query: 274 MVVVGIGIRPNTSLFDGQLTMEKGGIKVNGKMQSSNSSVYAVGDVAAFPVKLFSETRRLE 333
           +VV G+G RP TSLF GQL  EKGGIK +G  ++S   VYA+GDVA FP+K++  TRR+E
Sbjct: 256 IVVAGVGARPATSLFKGQLEEEKGGIKTDGFFKTSVPDVYALGDVATFPMKMYGGTRRVE 315

Query: 334 HVDSARKSARHAVSAIMDPEK---TGDFDDVPFFYSRVFTLSWQFYGDNIGEAVHFGDFS 393
           H D+ARKSA  AV AI   E+     D+D +P+FYSR F LSW+FYG+N+GE+V FGD  
Sbjct: 316 HADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDND 375

Query: 394 GNT----FGAYWVNKGHLVGSFLEGGTKEEYEAIAMITSLKPAIEDLAKLESQGLSYA 417
             +    FG YWV  G +VG FLEGGT+EE++AIA +   +P++E L  L  +GLS+A
Sbjct: 376 PKSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFA 433

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g23440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LK94	1.0e-172	67.15	Monodehydroascorbate reductase 4, peroxisomal OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q8S3R2	7.2e-158	61.46	Monodehydroascorbate reductase 2, peroxisomal OS=Oryza sativa subsp. japonica OX...	[more]
Q8S3R1	1.3e-135	56.43	Monodehydroascorbate reductase 1, peroxisomal OS=Oryza sativa subsp. japonica OX...	[more]
Q652L6	1.6e-104	47.38	Monodehydroascorbate reductase 3, cytosolic OS=Oryza sativa subsp. japonica OX=3...	[more]
Q42711	3.0e-103	47.38	Monodehydroascorbate reductase, seedling isozyme OS=Cucumis sativus OX=3659 PE=2...	[more]

BLAST of Cp4.1LG01g23440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023515751.1	1.82e-304	90.97	monodehydroascorbate reductase 4, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
XP_022921982.1	2.68e-291	88.24	monodehydroascorbate reductase 4, peroxisomal-like isoform X1 [Cucurbita moschat...	[more]
XP_022989902.1	1.55e-290	88.24	monodehydroascorbate reductase 4, peroxisomal-like isoform X1 [Cucurbita maxima]	[more]
XP_022921992.1	3.61e-278	96.78	monodehydroascorbate reductase 4, peroxisomal-like isoform X2 [Cucurbita moschat...	[more]
XP_022989904.1	1.47e-277	96.54	monodehydroascorbate reductase 4, peroxisomal-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG01g23440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1V8	1.30e-291	88.24	monodehydroascorbate reductase 4, peroxisomal-like isoform X1 OS=Cucurbita mosch...	[more]
A0A6J1JH36	7.49e-291	88.24	monodehydroascorbate reductase 4, peroxisomal-like isoform X1 OS=Cucurbita maxim...	[more]
A0A6J1E1W5	1.75e-278	96.78	monodehydroascorbate reductase 4, peroxisomal-like isoform X2 OS=Cucurbita mosch...	[more]
A0A6J1JQN0	7.11e-278	96.54	monodehydroascorbate reductase 4, peroxisomal-like isoform X2 OS=Cucurbita maxim...	[more]
A0A0A0KUG3	2.29e-271	84.60	Pyr_redox_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524740...	[more]

BLAST of Cp4.1LG01g23440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27820.1	7.4e-174	67.15	monodehydroascorbate reductase 4	[more]
AT5G03630.1	1.1e-103	47.72	Pyridine nucleotide-disulphide oxidoreductase family protein	[more]
AT3G52880.1	6.8e-103	45.48	monodehydroascorbate reductase 1	[more]
AT3G52880.2	3.4e-102	45.56	monodehydroascorbate reductase 1	[more]
AT3G09940.1	6.2e-96	45.45	monodehydroascorbate reductase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00469	PNDRDTASEII	coord: 279..297 score: 26.44 coord: 108..116 score: 55.56 coord: 153..177 score: 26.63 coord: 246..267 score: 30.82
None	No IPR available	PRINTS	PR00368	FADPNR	coord: 244..260 score: 37.68 coord: 269..291 score: 34.14 coord: 157..175 score: 39.01 coord: 107..125 score: 33.23
None	No IPR available	PANTHER	PTHR43557	APOPTOSIS-INDUCING FACTOR 1	coord: 76..448 coord: 33..61
None	No IPR available	PANTHER	PTHR43557:SF16	FAD/NAD-LINKED REDUCTASE, DIMERIZATION DOMAIN, FAD/NAD(P)-BINDING DOMAIN PROTEIN-RELATED	coord: 76..448 coord: 33..61
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 121..252 e-value: 1.8E-82 score: 278.8
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 74..317 e-value: 1.8E-82 score: 278.8
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 73..194
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 158..323
IPR016156	FAD/NAD-linked reductase, dimerisation domain superfamily	GENE3D	3.30.390.30		coord: 335..419 e-value: 1.9E-32 score: 113.2
IPR016156	FAD/NAD-linked reductase, dimerisation domain superfamily	SUPERFAMILY	55424	FAD/NAD-linked reductases, dimerisation (C-terminal) domain	coord: 329..396
IPR023753	FAD/NAD(P)-binding domain	PFAM	PF07992	Pyr_redox_2	coord: 45..314 e-value: 9.8E-48 score: 162.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g23440.1	Cp4.1LG01g23440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042744	hydrogen peroxide catabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005778	peroxisomal membrane
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016656	monodehydroascorbate reductase (NADH) activity
molecular_function	GO:0016491	oxidoreductase activity