Cp4.1LG01g23270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g23270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRNA exonuclease 4
LocationCp4.1LG01: 19714336 .. 19716111 (+)
RNA-Seq ExpressionCp4.1LG01g23270
SyntenyCp4.1LG01g23270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCACAAATGTGCAGCTTGCTTCAGGCAGTTCAACAAGATAGAGCACTTGGTAGAGCATATGAGACTCTCGTTTCACTCACTTCATGAACCAACTTGCTCCCTTTGCCACAAACACTGCAGATCCTTTGAATCTCTCAGAGAACATCTCATTGGTACGCCAAAGTTTCCATTTTTAGTTTTAAAATTACGATATTTAAGCTTATAAACACTACTTTCATCAGGGCCTCTGCCGAAGCAAGAATGCAACAACATATTCGGCACGAGAGGATGCAAAATCTGCTTAGCCATCTTTGAGAGTCCTCACACCTTGAGGCTTCACCAAGAAAGATGTCAACTTCGATCTCCAAATTCCGTATGTGAAAAATGTTTTTCATCAACGGTAAAAACTCAAGTTCACTTCTATCAAATATTGTCCTTCAAGGCTTTTAAAACGTGTCTATTAGGGAGAGGTTTCCACACCCTTATAAAAAAATGTTTCGTTCTCCTCTTCAACTGATGTGGGATCTCACAGTAATGTATCAAAGTCAGACACGGGACGACGTGCCAATAAGGAGGCTCCCAAAGGGGTTTGGCACAAGGCAGTGTGCCAACAAGAACACTGACCCCAAGGAGGTAAATTGGGGGGTCCCACAACGATTAGAGAAGGGAACGAGTGTTAGTGAGGACGCTGGGTCCTGAAGAGGGTAGATCGTGAGATCCCACATTGTTTGGAGAGAAGAACAAAGCAACTTTTATAAGGACGTGAAAATGGGGGCGAAAAAAGAAAGAGAGGGATGGAGTTTCTCTCGCTTTTGGCATAGCGGGCCCCAGCAGGAGTCCGCACGACGGGCTATTAGCTCAATGGTAGAGCGCGCCCTACATAATTGTGTTGTTGTGCTTGGGCTATGAGGGCTCTCAGCCACATGGAAAACCTCTCCTTAGCATAGCTTTGAAGGAAAAATTCAAAAGGGAAAGGATAAAAAGGACAATATCTACTAGCAGTACGGTGGTGCGTGCGTGGTGGTGTGTGGTGGTGTGTGCTTGTTGGGTTGAAATGCGTTTTATTTCTTATTTGACATCAAAATTGCTTCCTTACAGAGTCAAACCATGGCCCGTTTTCGCAACATGGGAATTTATGACAAATTGACGAGCGACAACGGCCGCATGAGGCGCCATGAAGCAGTGGCATTAGCTTGCAGAATGGTGGGCGGTGGCAGCGATGGGTCGTTAGATATATGCGCAAGGGTCTGCCTGATTGACGAAAACGAAACCATCATTTTCTCTACTTATGTCAACCCAACGCTCCCCATCACAAACTATAGGTACTACAACACATCCCAACTTTAGAACTAAACTAACCCAAAGGGCTCATCTTTTGAATTATCATATGCAGGTTGGAGTCCACGGGCGTTGGTCCAGAATACATGAGGGATCATGCAATGCCATTGAAGCAAGTTGGGAAGAAAATTGAAGACATCCTGTGCAATGGTGAGCCGATGTGGAAGATTCGACCTGGGGTGGGAGGGAAAGCAAGGATTCTTGTGGGTCATGGCTTGGGAGATCACCTCAAAAGCTTGCAAATTGAGTATCCACCATTCATGATAAGGTACATACTTTGAGTTCTTTCCATATATGAAACTTTGGTTTTAGTTTTTTTTTATATGAATTTTTCACAGGGACACTGCCTACTATCCACCATTGATGAAAACAAGCAAGCTCACCAATTCTCTCCACTATCTAACACAAGCATATCTTGGGCAAGTTCCACATAACCCTACCTACCTCATAATTCATCCT

mRNA sequence

GCACAAATGTGCAGCTTGCTTCAGGCAGTTCAACAAGATAGAGCACTTGGTAGAGCATATGAGACTCTCGTTTCACTCACTTCATGAACCAACTTGCTCCCTTTGCCACAAACACTGCAGATCCTTTGAATCTCTCAGAGAACATCTCATTGGTACGCCAAAGTTTCCATTTTTAAGTCAAACCATGGCCCGTTTTCGCAACATGGGAATTTATGACAAATTGACGAGCGACAACGGCCGCATGAGGCGCCATGAAGCAGTGGCATTAGCTTGCAGAATGGTGGGCGGTGGCAGCGATGGGTCGTTAGATATATGCGCAAGGGTCTGCCTGATTGACGAAAACGAAACCATCATTTTCTCTACTTATGTCAACCCAACGCTCCCCATCACAAACTATAGGTTGGAGTCCACGGGCGTTGGTCCAGAATACATGAGGGATCATGCAATGCCATTGAAGCAAGTTGGGAAGAAAATTGAAGACATCCTGTGCAATGGTGAGCCGATGTGGAAGATTCGACCTGGGGTGGGAGGGAAAGCAAGGATTCTTGTGGGTCATGGCTTGGGAGATCACCTCAAAAGCTTGCAAATTGAGTATCCACCATTCATGATAAGGGACACTGCCTACTATCCACCATTGATGAAAACAAGCAAGCTCACCAATTCTCTCCACTATCTAACACAAGCATATCTTGGGCAAGTTCCACATAACCCTACCTACCTCATAATTCATCCT

Coding sequence (CDS)

ATGAGACTCTCGTTTCACTCACTTCATGAACCAACTTGCTCCCTTTGCCACAAACACTGCAGATCCTTTGAATCTCTCAGAGAACATCTCATTGGTACGCCAAAGTTTCCATTTTTAAGTCAAACCATGGCCCGTTTTCGCAACATGGGAATTTATGACAAATTGACGAGCGACAACGGCCGCATGAGGCGCCATGAAGCAGTGGCATTAGCTTGCAGAATGGTGGGCGGTGGCAGCGATGGGTCGTTAGATATATGCGCAAGGGTCTGCCTGATTGACGAAAACGAAACCATCATTTTCTCTACTTATGTCAACCCAACGCTCCCCATCACAAACTATAGGTTGGAGTCCACGGGCGTTGGTCCAGAATACATGAGGGATCATGCAATGCCATTGAAGCAAGTTGGGAAGAAAATTGAAGACATCCTGTGCAATGGTGAGCCGATGTGGAAGATTCGACCTGGGGTGGGAGGGAAAGCAAGGATTCTTGTGGGTCATGGCTTGGGAGATCACCTCAAAAGCTTGCAAATTGAGTATCCACCATTCATGATAAGGGACACTGCCTACTATCCACCATTGATGAAAACAAGCAAGCTCACCAATTCTCTCCACTATCTAACACAAGCATATCTTGGGCAAGTTCCACATAACCCTACCTACCTCATAATTCATCCT

Protein sequence

MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGTPKFPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHYLTQAYLGQVPHNPTYLIIHP
Homology
BLAST of Cp4.1LG01g23270 vs. ExPASy Swiss-Prot
Match: Q9GZR2 (RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 5.4e-15
Identity = 51/147 (34.69%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 67  AVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMR 126
           A+AL C MVG G  G   + ARV ++++    ++  YV PT P+T+YR   +G+ PE ++
Sbjct: 243 ALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRPENLK 302

Query: 127 DHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRD 186
                L+ V K++ ++L               K RILVGH L + LK L +++P   IRD
Sbjct: 303 -QGEELEVVQKEVAEML---------------KGRILVGHALHNDLKVLFLDHPKKKIRD 362

Query: 187 TAYYPPLMKTSKLTN-SLHYLTQAYLG 213
           T  Y P     K    SL  L++  LG
Sbjct: 363 TQKYKPFKSQVKSGRPSLRLLSEKILG 373

BLAST of Cp4.1LG01g23270 vs. ExPASy Swiss-Prot
Match: Q6PAQ4 (RNA exonuclease 4 OS=Mus musculus OX=10090 GN=Rexo4 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.1e-14
Identity = 50/148 (33.78%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 66  EAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYM 125
           +A+AL C MVG G  G   I ARV ++++    ++  YV PT P+T+YR   +G+ PE +
Sbjct: 252 KALALDCEMVGVGPKGEESIAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRPENL 311

Query: 126 RDHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIR 185
           +      + V K++ ++L               K RILVGH L + LK L +++P   IR
Sbjct: 312 K-QGEEFEVVKKEVAEML---------------KGRILVGHALHNDLKVLFLDHPKKKIR 371

Query: 186 DTAYYPPLMKTSKLTN-SLHYLTQAYLG 213
           DT  + P     K    SL  L++  LG
Sbjct: 372 DTQKFKPFRSRVKSGRPSLKRLSEKILG 383

BLAST of Cp4.1LG01g23270 vs. ExPASy Swiss-Prot
Match: Q91560 (RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 3.0e-13
Identity = 45/131 (34.35%), Postives = 67/131 (51.15%), Query Frame = 0

Query: 68  VALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRD 127
           VA+ C MVG G DG   I ARV +++     ++  YV PT  +T+YR   +G+ PE ++ 
Sbjct: 235 VAMDCEMVGVGMDGEESILARVSIVNLFGKCVYDKYVKPTERVTDYRTAVSGIRPEDVK- 294

Query: 128 HAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDT 187
              P K V K++ +IL               + R LVGH + + LK L +++P   IRDT
Sbjct: 295 KGEPFKVVQKEVSEIL---------------RGRTLVGHAVHNDLKILFLDHPKKAIRDT 349

Query: 188 AYYPPLMKTSK 199
             Y P  +  K
Sbjct: 355 QKYKPFKQKVK 349

BLAST of Cp4.1LG01g23270 vs. ExPASy Swiss-Prot
Match: Q4PER6 (RNA exonuclease 4 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=REX4 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.7e-13
Identity = 51/172 (29.65%), Postives = 85/172 (49.42%), Query Frame = 0

Query: 44  ARFRNMGIYDKLTSDNGRMRRHEA--VALACRMVGGGSDGSLDICARVCLIDENETIIFS 103
           A+ +   I   L SD    ++     +A+ C MVG G  GS  I ARV +++ +   I+ 
Sbjct: 110 AKLKKQIILGGLPSDASSAKKEPGNYLAIDCEMVGVGDKGSESILARVSIVNFHGATIYD 169

Query: 104 TYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKAR 163
            +V P   +T+YR   +GV P+ ++  A    QV  ++ +++               K +
Sbjct: 170 QFVRPQEKVTDYRTWVSGVRPKDLKG-APSFSQVQGEVANLI---------------KGK 229

Query: 164 ILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLT-NSLHYLTQAYLG 213
           +LVGH + + LK+L + +P  +IRDTA + PL   +K    SL  L +  LG
Sbjct: 230 VLVGHAIQNDLKALLLSHPKVLIRDTATFQPLRDLAKTKYPSLKKLAKLVLG 265

BLAST of Cp4.1LG01g23270 vs. ExPASy Swiss-Prot
Match: P0CQ45 (RNA exonuclease 4 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=REX4 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.6e-11
Identity = 44/159 (27.67%), Postives = 81/159 (50.94%), Query Frame = 0

Query: 40  SQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETII 99
           S  +   R+M + + L S++ +    + +A+ C MVG G +G  +  ARV +++ +  +I
Sbjct: 106 SPLLHELRHMVLGNHLLSES-QKEPGQYLAIDCEMVGVGPNGMENTLARVSIVNYHGAVI 165

Query: 100 FSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGK 159
             T+V P  P+T+YR   +GV    +   A    +V K++ ++L +              
Sbjct: 166 LDTFVQPREPVTDYRTWISGVKQSDLLG-APQFDEVNKQVANLLHD-------------- 225

Query: 160 ARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSK 199
            +IL+GH + + LK L + +P  + RDT  Y PL + +K
Sbjct: 226 -KILIGHAIDNDLKVLMLTHPGPLTRDTQKYKPLQEIAK 247

BLAST of Cp4.1LG01g23270 vs. NCBI nr
Match: XP_023543310.1 (RNA exonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 410 bits (1055), Expect = 6.90e-142
Identity = 208/248 (83.87%), Postives = 208/248 (83.87%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPLPKQECNNIFGTRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNSSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC
Sbjct: 149 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 212
           NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 268

BLAST of Cp4.1LG01g23270 vs. NCBI nr
Match: XP_022990586.1 (RNA exonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 393 bits (1010), Expect = 4.82e-135
Identity = 200/248 (80.65%), Postives = 203/248 (81.85%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPLPKQECNNIFGTRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQ MARFRNMGI DKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNFSQIMARFRNMGICDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDE ETIIFSTYVNPTLPITNYRLESTGVGPEY+RDHAMPLKQV KKI+DILC
Sbjct: 149 ICARVCLIDEKETIIFSTYVNPTLPITNYRLESTGVGPEYLRDHAMPLKQVQKKIQDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 212
           NGEPMWKIRPGVGGKARILVGHGLGDHLK+LQIEYPPFMIRDTA YPPLMKTSKLTNSLH
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKTLQIEYPPFMIRDTANYPPLMKTSKLTNSLH 268

BLAST of Cp4.1LG01g23270 vs. NCBI nr
Match: XP_022961916.1 (RNA exonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 390 bits (1002), Expect = 7.66e-134
Identity = 200/248 (80.65%), Postives = 201/248 (81.05%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPRPKQECNNVFVIRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQ   RFRNMGI DKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNSSQITTRFRNMGICDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRD AMPLKQV KKI+DILC
Sbjct: 149 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDRAMPLKQVQKKIQDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 212
           NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTA YPPLMKTSKLTNSLH
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTANYPPLMKTSKLTNSLH 268

BLAST of Cp4.1LG01g23270 vs. NCBI nr
Match: KAG7033124.1 (RNA exonuclease 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348 bits (892), Expect = 5.05e-118
Identity = 173/187 (92.51%), Postives = 174/187 (93.05%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PKFPFL-SQTMARFRNMGIYDKLTSD 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK     SQ   RFRNMGI DKLTSD
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPRPKQECNNSQITTRFRNMGICDKLTSD 88

Query: 61  NGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLEST 120
           NGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLEST
Sbjct: 89  NGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRLEST 148

Query: 121 GVGPEYMRDHAMPLKQVGKKIEDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIE 180
           GVGPEYMRD AMPLKQV KKI+DILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIE
Sbjct: 149 GVGPEYMRDRAMPLKQVQKKIQDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIE 208

Query: 181 YPPFMIR 185
           YPPFMIR
Sbjct: 209 YPPFMIR 215

BLAST of Cp4.1LG01g23270 vs. NCBI nr
Match: KAG6602442.1 (hypothetical protein SDJN03_07675, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336 bits (862), Expect = 9.29e-113
Identity = 173/221 (78.28%), Postives = 174/221 (78.73%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPRPKQECNNIFVIRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQ    FRN GI DKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNSSQITTPFRNTGICDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQV KKI+DILC
Sbjct: 149 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVQKKIQDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIR 185
           NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIR
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIR 249

BLAST of Cp4.1LG01g23270 vs. ExPASy TrEMBL
Match: A0A6J1JNC8 (RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111487420 PE=3 SV=1)

HSP 1 Score: 393 bits (1010), Expect = 2.33e-135
Identity = 200/248 (80.65%), Postives = 203/248 (81.85%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPLPKQECNNIFGTRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQ MARFRNMGI DKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNFSQIMARFRNMGICDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDE ETIIFSTYVNPTLPITNYRLESTGVGPEY+RDHAMPLKQV KKI+DILC
Sbjct: 149 ICARVCLIDEKETIIFSTYVNPTLPITNYRLESTGVGPEYLRDHAMPLKQVQKKIQDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 212
           NGEPMWKIRPGVGGKARILVGHGLGDHLK+LQIEYPPFMIRDTA YPPLMKTSKLTNSLH
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKTLQIEYPPFMIRDTANYPPLMKTSKLTNSLH 268

BLAST of Cp4.1LG01g23270 vs. ExPASy TrEMBL
Match: A0A6J1HBP2 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111462538 PE=3 SV=1)

HSP 1 Score: 390 bits (1002), Expect = 3.71e-134
Identity = 200/248 (80.65%), Postives = 201/248 (81.05%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG  PK                        
Sbjct: 29  MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGPRPKQECNNVFVIRGCKICLAIFESPHT 88

Query: 61  -----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 120
                       P  SQ   RFRNMGI DKLTSDNGRMRRHEAVALACRMVGGGSDGSLD
Sbjct: 89  LRLHQERCQLRSPNSSQITTRFRNMGICDKLTSDNGRMRRHEAVALACRMVGGGSDGSLD 148

Query: 121 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILC 180
           ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRD AMPLKQV KKI+DILC
Sbjct: 149 ICARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDRAMPLKQVQKKIQDILC 208

Query: 181 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLH 212
           NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTA YPPLMKTSKLTNSLH
Sbjct: 209 NGEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTANYPPLMKTSKLTNSLH 268

BLAST of Cp4.1LG01g23270 vs. ExPASy TrEMBL
Match: A0A251QA67 (C2H2-type domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G285000 PE=4 SV=1)

HSP 1 Score: 290 bits (741), Expect = 1.83e-95
Identity = 154/251 (61.35%), Postives = 176/251 (70.12%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK--------------------FPFL 60
           MR S+HS HEPTC +C KHCRSFESLREHLIG  PK                     P+ 
Sbjct: 32  MRTSYHSAHEPTCGICKKHCRSFESLREHLIGPLPKQECRNIFSTRGCKFCLAILDSPYA 91

Query: 61  SQT--------------MARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDI 120
            +               + RF N+GI D   ++NG  R  + VALAC+MVGGGSDGSLD+
Sbjct: 92  LRVHQDRCQLSGVNAGLLGRFANLGIRDN--TENGSTRGTQVVALACKMVGGGSDGSLDL 151

Query: 121 CARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCN 180
           CA+VCLIDE E IIF +YV P LP+TNYR E+TG+ PEY+RD AMPL+QV KKI+D LCN
Sbjct: 152 CAKVCLIDEYENIIFQSYVKPLLPVTNYRYETTGIRPEYLRD-AMPLRQVQKKIQDFLCN 211

Query: 181 GEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHY 216
           GEPMWKIRP  GGKARILVGHGL   L SLQ+EYP  MIRDTA YPPLMKTSKL+NSL Y
Sbjct: 212 GEPMWKIRPR-GGKARILVGHGLDHDLDSLQVEYPQVMIRDTAKYPPLMKTSKLSNSLKY 271

BLAST of Cp4.1LG01g23270 vs. ExPASy TrEMBL
Match: A0A7J7D2A0 (RNA exonuclease 4 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW11G00437 PE=3 SV=1)

HSP 1 Score: 291 bits (744), Expect = 4.63e-95
Identity = 148/246 (60.16%), Postives = 172/246 (69.92%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK------------------------ 60
           MR S+HS+HEPTC +C KHCRSFESLREHLIG  PK                        
Sbjct: 29  MRTSYHSIHEPTCGICKKHCRSFESLREHLIGPLPKQECKNIFSVRGCKFCLTVLDSPNA 88

Query: 61  ---------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDIC 120
                    F   +   ARF N+G+ D LT DNG MR ++ VALAC+MVGGGSDGSLD+C
Sbjct: 89  CRFHQDRCQFSVANGLAARFSNIGLRDNLTIDNGVMRGNQVVALACKMVGGGSDGSLDLC 148

Query: 121 ARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNG 180
           ARVC++DENE IIF +YV P +P+TNYR ESTG+ PEY+RD AMPL+QV +KI+D LCNG
Sbjct: 149 ARVCIVDENENIIFHSYVKPPIPVTNYRYESTGIRPEYLRD-AMPLRQVQRKIQDFLCNG 208

Query: 181 EPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHYL 212
           E MW+I+P   G ARILVGHGL   L  LQ+EYP  MIRDTA YPPLMKTSKL+NSL YL
Sbjct: 209 EAMWRIQPRNSGTARILVGHGLDHDLDRLQLEYPSIMIRDTAKYPPLMKTSKLSNSLRYL 268

BLAST of Cp4.1LG01g23270 vs. ExPASy TrEMBL
Match: A0A1R3HH88 (RNA exonuclease 4 OS=Corchorus olitorius OX=93759 GN=COLO4_28968 PE=3 SV=1)

HSP 1 Score: 290 bits (742), Expect = 9.60e-95
Identity = 151/247 (61.13%), Postives = 173/247 (70.04%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGT-PK--------------------FPFL 60
           MR+S+HS+HEPTC +C KHCRSFESLREHLIG  PK                     P+ 
Sbjct: 28  MRVSYHSVHEPTCGVCKKHCRSFESLREHLIGPLPKQECKSVFNIRGCRFCLAILDSPYA 87

Query: 61  SQT--------------MARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDI 120
            +               +AR  N+G+ D LT DNG  R  + VALAC+MVGGGSDGSLD+
Sbjct: 88  LRIHQDRCQLSGVNYGILARLANIGLRDSLTIDNGYSRGSQVVALACKMVGGGSDGSLDL 147

Query: 121 CARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCN 180
           CARVCLIDENE IIF TYV P +P+TNYR ESTG+ PEY+RD AMPL+QV K+I+D LCN
Sbjct: 148 CARVCLIDENENIIFHTYVKPQIPVTNYRYESTGIRPEYLRD-AMPLRQVQKRIQDFLCN 207

Query: 181 GEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHY 212
           GE MWKIR   GG+ARILVGHGL   L  LQ+EYP  MIRDTA YPPLMKTSKL+NSL Y
Sbjct: 208 GEAMWKIRSPKGGRARILVGHGLDHDLDRLQVEYPAIMIRDTAKYPPLMKTSKLSNSLKY 267

BLAST of Cp4.1LG01g23270 vs. TAIR 10
Match: AT3G27970.1 (Exonuclease family protein )

HSP 1 Score: 266.2 bits (679), Expect = 2.5e-71
Identity = 138/251 (54.98%), Postives = 167/251 (66.53%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG------------------------TPKF 60
           M++S+HS HEPTC +C KHCRSFESLREHLIG                        +P  
Sbjct: 34  MKISYHSGHEPTCGVCKKHCRSFESLREHLIGPLPKQECKNIFSLRGCRFCMTILESPNS 93

Query: 61  PFLSQTMARFRN-----------MGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDI 120
             + Q   +F +           +G+ DK   D    R    VAL+C+MVGGGSDGSLD+
Sbjct: 94  RRIHQERCQFSSVNSGLTTRMAALGLRDKAMIDYTSSRSPRVVALSCKMVGGGSDGSLDL 153

Query: 121 CARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCN 180
           CARVC+ DE++ +IF TYV P++ +T+YR E+TG+ PE +RD AMPLKQV +KI++ LCN
Sbjct: 154 CARVCITDESDNVIFHTYVKPSMAVTSYRYETTGIRPENLRD-AMPLKQVQRKIQEFLCN 213

Query: 181 GEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHY 217
           GEPMWKIRP  GGKARILVGHGL   L  LQ+EYP  MIRDTA YPPLMKTSKL+NSL Y
Sbjct: 214 GEPMWKIRPR-GGKARILVGHGLDHDLDRLQLEYPSSMIRDTAKYPPLMKTSKLSNSLKY 273

BLAST of Cp4.1LG01g23270 vs. TAIR 10
Match: AT5G40310.1 (Exonuclease family protein )

HSP 1 Score: 265.4 bits (677), Expect = 4.2e-71
Identity = 137/251 (54.58%), Postives = 165/251 (65.74%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIG-TPK------------------------ 60
           MR+S+HS+HEPTC +C+KHCRSF+SLREHLIG  PK                        
Sbjct: 25  MRISYHSVHEPTCGICNKHCRSFDSLREHLIGPLPKQECKNIFSIRGCRFCLTILESPNA 84

Query: 61  ----------FPFLSQTMARFRNMGIYDKLTSDNGRMRRHEAVALACRMVGGGSDGSLDI 120
                         S  M R   +G+ +  T D    R    VAL+C+MVGGGSDGSLD+
Sbjct: 85  RRIHQERCQLSNVTSGLMIRMAALGLRNNSTIDYTSSRSPRVVALSCKMVGGGSDGSLDL 144

Query: 121 CARVCLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCN 180
           CARVC+ DE+E ++F TYV PT+P+TNYR E TG+ PE +RD AM LK   +K+++ LCN
Sbjct: 145 CARVCITDESENVVFHTYVKPTIPVTNYRYEMTGIRPENLRD-AMRLKHAQRKVQEFLCN 204

Query: 181 GEPMWKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHY 217
           GEPMWKIRP   GKARILVGHGL +HL SLQ+EY   MIRDTA YPPLMK+SKL+NSL Y
Sbjct: 205 GEPMWKIRPR-NGKARILVGHGLDNHLDSLQLEYSSSMIRDTAEYPPLMKSSKLSNSLKY 264

BLAST of Cp4.1LG01g23270 vs. TAIR 10
Match: AT2G48100.1 (Exonuclease family protein )

HSP 1 Score: 174.9 bits (442), Expect = 7.5e-44
Identity = 97/243 (39.92%), Postives = 139/243 (57.20%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGTPKFPFLSQTMARFRNMG------IYDK 60
           M++S+HSLH+P C +C KHC+SFES+REHL   P         A F   G      I+++
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHL-NVPDHLSKGNCKAIFTKRGCTLCLQIFEE 95

Query: 61  L-------------------------TSDNGRMRRHEAVALACRMVGGGSDGSLDICARV 120
                                      S +    R +A+AL C MVGGG+DG++D CA V
Sbjct: 96  AFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSRLKAMALDCEMVGGGADGTIDQCASV 155

Query: 121 CLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPM 180
           CL+D++E +IFST+V P LP+T+YR E TG+  E ++D  MPL+ V +++   LC G+  
Sbjct: 156 CLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKD-GMPLEHVRERVFSFLCGGQ-- 215

Query: 181 WKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHYLTQA 213
                  G    +LVGH L   +  L++EYP  ++RDTA Y PLMKT+ ++ SL YLT++
Sbjct: 216 -----NDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKS 269

BLAST of Cp4.1LG01g23270 vs. TAIR 10
Match: AT2G48100.2 (Exonuclease family protein )

HSP 1 Score: 174.9 bits (442), Expect = 7.5e-44
Identity = 97/243 (39.92%), Postives = 139/243 (57.20%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGTPKFPFLSQTMARFRNMG------IYDK 60
           M++S+HSLH+P C +C KHC+SFES+REHL   P         A F   G      I+++
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHL-NVPDHLSKGNCKAIFTKRGCTLCLQIFEE 95

Query: 61  L-------------------------TSDNGRMRRHEAVALACRMVGGGSDGSLDICARV 120
                                      S +    R +A+AL C MVGGG+DG++D CA V
Sbjct: 96  AFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSRLKAMALDCEMVGGGADGTIDQCASV 155

Query: 121 CLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPM 180
           CL+D++E +IFST+V P LP+T+YR E TG+  E ++D  MPL+ V +++   LC G+  
Sbjct: 156 CLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKD-GMPLEHVRERVFSFLCGGQ-- 215

Query: 181 WKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHYLTQA 213
                  G    +LVGH L   +  L++EYP  ++RDTA Y PLMKT+ ++ SL YLT++
Sbjct: 216 -----NDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKS 269

BLAST of Cp4.1LG01g23270 vs. TAIR 10
Match: AT2G48100.3 (Exonuclease family protein )

HSP 1 Score: 174.9 bits (442), Expect = 7.5e-44
Identity = 97/243 (39.92%), Postives = 139/243 (57.20%), Query Frame = 0

Query: 1   MRLSFHSLHEPTCSLCHKHCRSFESLREHLIGTPKFPFLSQTMARFRNMG------IYDK 60
           M++S+HSLH+P C +C KHC+SFES+REHL   P         A F   G      I+++
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHL-NVPDHLSKGNCKAIFTKRGCTLCLQIFEE 95

Query: 61  L-------------------------TSDNGRMRRHEAVALACRMVGGGSDGSLDICARV 120
                                      S +    R +A+AL C MVGGG+DG++D CA V
Sbjct: 96  AFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSRLKAMALDCEMVGGGADGTIDQCASV 155

Query: 121 CLIDENETIIFSTYVNPTLPITNYRLESTGVGPEYMRDHAMPLKQVGKKIEDILCNGEPM 180
           CL+D++E +IFST+V P LP+T+YR E TG+  E ++D  MPL+ V +++   LC G+  
Sbjct: 156 CLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKD-GMPLEHVRERVFSFLCGGQ-- 215

Query: 181 WKIRPGVGGKARILVGHGLGDHLKSLQIEYPPFMIRDTAYYPPLMKTSKLTNSLHYLTQA 213
                  G    +LVGH L   +  L++EYP  ++RDTA Y PLMKT+ ++ SL YLT++
Sbjct: 216 -----NDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKS 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9GZR25.4e-1534.69RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2[more]
Q6PAQ42.1e-1433.78RNA exonuclease 4 OS=Mus musculus OX=10090 GN=Rexo4 PE=2 SV=2[more]
Q915603.0e-1334.35RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2[more]
Q4PER68.7e-1329.65RNA exonuclease 4 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=REX4 ... [more]
P0CQ453.6e-1127.67RNA exonuclease 4 OS=Cryptococcus neoformans var. neoformans serotype D (strain ... [more]
Match NameE-valueIdentityDescription
XP_023543310.16.90e-14283.87RNA exonuclease 4-like [Cucurbita pepo subsp. pepo][more]
XP_022990586.14.82e-13580.65RNA exonuclease 4-like [Cucurbita maxima][more]
XP_022961916.17.66e-13480.65RNA exonuclease 4-like [Cucurbita moschata][more]
KAG7033124.15.05e-11892.51RNA exonuclease 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602442.19.29e-11378.28hypothetical protein SDJN03_07675, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1JNC82.33e-13580.65RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111487420 PE=3 SV=1[more]
A0A6J1HBP23.71e-13480.65RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111462538 PE=3 SV=1[more]
A0A251QA671.83e-9561.35C2H2-type domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G285000 ... [more]
A0A7J7D2A04.63e-9560.16RNA exonuclease 4 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW11G00437 PE=3 S... [more]
A0A1R3HH889.60e-9561.13RNA exonuclease 4 OS=Corchorus olitorius OX=93759 GN=COLO4_28968 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G27970.12.5e-7154.98Exonuclease family protein [more]
AT5G40310.14.2e-7154.58Exonuclease family protein [more]
AT2G48100.17.5e-4439.92Exonuclease family protein [more]
AT2G48100.27.5e-4439.92Exonuclease family protein [more]
AT2G48100.37.5e-4439.92Exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 66..219
e-value: 0.0012
score: 13.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 62..219
e-value: 3.9E-25
score: 90.4
NoneNo IPR availablePANTHERPTHR12801RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATEDcoord: 1..33
coord: 42..212
NoneNo IPR availablePANTHERPTHR12801:SF122OS02G0709000 PROTEINcoord: 1..33
coord: 42..212
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 80..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g23270.1Cp4.1LG01g23270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding