Cp4.1LG01g22520 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g22520
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCyclic nucleotide gated channel
LocationCp4.1LG01: 20101863 .. 20105679 (-)
RNA-Seq ExpressionCp4.1LG01g22520
SyntenyCp4.1LG01g22520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAACATTATTTGAAGTGTTGAGAATAATATTGGAGATACGGAAGCATAAAAGTGAAGCCCCCACCACAGCAACACCTCCCCTTACCTTACGTGATTATTCCTCATCTTCTACTTCTTCTTCTTCTGCTGAATTTGCTTCCACTCTCTCCCATGGTGGTTCTGGTTCCTTCATTCTCCCCATGCCTTCTCTCCCTGGCTTCCCCTTCTCCTCTCCCATGTCTGTAATCTTTTTCACTTCTCCTTTAATCATTTCTTCTCCTCTGTTTTTCCCTTTTACTCATTTCATTTCTTTCCAATTTGCAGGTGGAGCGAGCTTTGCCTGAGGCGAACCACCCCTTCACTCAATGCCCACGACAACATTAACAACACCACCACCGCTGCCGATGAACACCCCATCTCCAACGCCGTTGAGTGCTACGCCTGCACCCAAGTCGGTGTTCCGGCCTTCCATTCCACCAGCTGTGACCATGCCCACCAGCAGCCCGAATGGGAGGCCTCCGCCGGCTCTTCCCTCGTCCCCATTCAACCCACAAAGACCTCTCCTCGCCGCGCCGACCCACAACATCGTTCCGGTCGCTTCGGCAGTGTTCTCGACCCTAGAACCAAGCGGGTCCAAAGATGGAACCGGGCTCTGTTATTAGCCCGTGGAATGGCCCTCGCCGTCGATCCTCTCTACTTCTACGCGCTCTCCATCGGACGTGGTGGGTCGCCGTGCCTGTACATGGACGGTGGATTAATCGCCGTTGTTACTGTTCTCCGCACGTTCCTCGATGTCGTGCACTTGTGGCACGTTTGGCTCCAGTTCCGCCTCGCCTATGTGTCCAAGGAGAGCATGGTCATTGGCTGCGGCAAACTCGTCTGGGACGCTCGTGATATCGCCTCCCACTATGTTCGTTCCTTCAAAGGCTTCTGGTTCGATGCCTTCGTGATCCTCCCAGTTCCTCAGGTTTGCCTTCTTCTTTAATCATTTTAAATAGAGCCCAATGATGATATGGGTAGTGTTCATTGATTGGAATTTGTAGTTAGTTAACTTGGAGAATCGCTTATTGATAGGGAATGAATTAAAATATATCTCAATCATTGCAAATAAACAATAGTTTGATCATAATAAGAACTAGCAACTCAAATTTTAGTGAGCTAAATCAATGATTTTCATATTTTATTCTAACTTTTCAAAACATGGTATGATCAAAATCCAATTATTGAAGTGTTTCTTTTGTTGTCTCTGCTTATCTCTTCTTATTGCAGATTGTTTATTGGTTGGTTTTACCAAAACTAATCAGAGAAGAGAGAATCAAGCTTATAATGACTGTAATCTTGTTAATGTTCTTGTTCCAATTCCTCCCAAAAGTTTACCACTCCATTATTTTAATGACAAGAATGCAAAAGGTCACTGGATACATCTTTGGCACCATTTGGTGGGGTTTTGGACTCAATCTCATTGCCTATTTCATCGCCTCTCATGTAAGCCTGCTTTGTATATTCATCACAGCCATCTCTTTTAAACTGAAATTTGCAAGCGCTTCTGGTTTTCCTGCTAACAGGTTGCTGGGGGTTGCTGGTATGTTCTTGCAATACAGCGAGTTGCTTCTTGCATCCAGCAACATTGTGAGAGAAACAAGTGCAACCTATCTCTGTCTTGCTCAGAGGAGGTGTGTTATCAGTTTCTCTCATCTTCTACAACCATAGGAAATTCGTGTGGTCGTAATTCGACTTCTGCCTTTAGGAAGCCCATATGCTTGGATGTTAATGGTCCATTCGCCTATGGCATCTACAAGTGGGCTCTTCCAGTCATTTCTAGCAATTCCATTGCTGTCAAAATTCTTTATCCCATCTTTTGGGGCTTAATGACTCTTAGGTAAGTATAGGAGCAGTAGTTTTCAAACTCTGTGTTCTGTTCCTGTGGAGAAAAGGGAAGAGTTTTCTACACTATGTTTCTTGATAATGATGGTTTAAATGTTGCAGCACGTTTGGCAATGATCTCGAGCCCACGAGTAATTGGCTAGAAGTATGTTTTAGTATTTGCACAGTACTCAGTGGATTGCTGCTTTTTACTCTTTTGATTGGTAACATCCAGGTGAGCATGCTTGCTCGTTCAATATGTTGTGCACATGTAGCTGAAAGTATTCTTCTTCTGTGGACCTTAACCTCGTCTCCCTTTTGCGAAAAAGGTTCTTTTGCACGCCGTCATGGCAAGGAGGCGAAAAATGCAGCTGAGATGTCGAGATTTAGAGTGGTGGATGAGGAGACGACAATTGCCATCTCGTTTGAGACATCGTGTTCGACACTATGAACACCAGAGATGGGCAGCCATGGGTGGAGAAGATGAGATGGAACTGATCAATGATTTGCCTGAAGGTCTTAGAAGAGACATTAAACGCCATCTTTGTGTTGATCTTATCAGAAAGGTAAATAGTTTGAATAGCTGTTCTCTTGAGGATCTCTTTAGGATTAGGGAAGGCTTGTGGTTGAGGTTCTGTTTCCTTATGTCTAACGGCCCAAATCCACCGCTAGTAGATATTGTCCTCTTTTGTCTTCCCCTTTTGGGCTTTCCTAAAAGATTTAAAAACACGTCTGCTAATGAGAGGTTTTCACACCCTTATAAAGAATGTTTCGTTCGGTAGAGGATCTCTTTTGGACTAGGGAAACTTGTGATTAAGGTTCTGTTTCCTTATGCAACTGTTACCAAATGCATAGGTGCCGCTGTTCCAAAACCTGGATGAGCTGATTCTCGACAACATCTGCGACCGAGTCAAGCCACTTGTATTTTCCAAAGATGAAAAGGTACATGTTCAAACCCGACTCGTCGGTGATCCGCTATTTACCAAGGATACACTAATATCAATGTTTCCATAAAAACAGATTATCAGAGAAGGAGATCCTGTTCCAAGAATGCTATTCATAGTGTGTGGACGTGTAAAACGTAACCAAAGCCTGAGCAAGGGCATGACAGCAACAAGTTTGCTAGAATCGGGAGGATTTCTGGGCGACGAGCTGCTATCGTGGTGTCTTCGGCGCCCATTTCTCGAGAGGCTTCCAGCTTCATCAGCTACATTTGTATGCATTGAACCAACAGAAGTATTTGCCCTGAAAGCAGACCATCTGAAGTACATAACCGATCACTTCCGCTACAAATTTGCCAACGAACGACTGAAGAGAACTGCAAGATATTACTCCTCCAACTGGAGAACATGGGCTGCTGTGAACATACAATTTGCTTGGCGTCGCTACAGAAAGAGGATGAGACGTCCAGTGATAACTGTGGTGGAAAATGGAAGCAATGAACGTAGGCTTCTGCAGTATGCTGCAATGTTCATGTCATTCAGGCCACATGACCATCTTGAATAGTAAACTATGAGCTTGCCTACACTTCTTGAATTCTTTCATAAGTCATTCATTGATATCATTTTCATATTATTCGATATCTATAATCTCCAAGGAAAGACATGGACTTGGAAGTTGGAAAATGGGATGTATTTGAGTGTAAAGAGTGAGATATGTTCATCTTTCTCCCTTTCATGTCTCTTCCCTCTCTCTTCCTCCGACATGACAGATCATGTTTCTCAACGAAGATGTGGAGTTGAAGATGAATGTATTGTTAAGAATGTTTTTAGTAAGAGAACGTGTCTAGATTGAGTAATGGCAGTTGTCGTCTCTCTTCCATCAACTCGTGAGCTCAACTAGTTAAGATATATTCCATTTGTTCGTGAATTACAGAAAAAAGTCCTATAGACATTGTGTTTGTTTCATTTGCTAATGGTTAAGATATCTCTTGTAAGATGTAAGAGCTCAGGCACCGCTAGGCTAGCAGATATC

mRNA sequence

GCAACATTATTTGAAGTGTTGAGAATAATATTGGAGATACGGAAGCATAAAAGTGAAGCCCCCACCACAGCAACACCTCCCCTTACCTTACGTGATTATTCCTCATCTTCTACTTCTTCTTCTTCTGCTGAATTTGCTTCCACTCTCTCCCATGGTGGTTCTGGTTCCTTCATTCTCCCCATGCCTTCTCTCCCTGGCTTCCCCTTCTCCTCTCCCATGTGGAGCGAGCTTTGCCTGAGGCGAACCACCCCTTCACTCAATGCCCACGACAACATTAACAACACCACCACCGCTGCCGATGAACACCCCATCTCCAACGCCGTTGAGTGCTACGCCTGCACCCAAGTCGGTGTTCCGGCCTTCCATTCCACCAGCTGTGACCATGCCCACCAGCAGCCCGAATGGGAGGCCTCCGCCGGCTCTTCCCTCGTCCCCATTCAACCCACAAAGACCTCTCCTCGCCGCGCCGACCCACAACATCGTTCCGGTCGCTTCGGCAGTGTTCTCGACCCTAGAACCAAGCGGGTCCAAAGATGGAACCGGGCTCTGTTATTAGCCCGTGGAATGGCCCTCGCCGTCGATCCTCTCTACTTCTACGCGCTCTCCATCGGACGTGGTGGGTCGCCGTGCCTGTACATGGACGGTGGATTAATCGCCGTTGTTACTGTTCTCCGCACGTTCCTCGATGTCGTGCACTTGTGGCACGTTTGGCTCCAGTTCCGCCTCGCCTATGTGTCCAAGGAGAGCATGGTCATTGGCTGCGGCAAACTCGTCTGGGACGCTCGTGATATCGCCTCCCACTATGTTCGTTCCTTCAAAGGCTTCTGGTTCGATGCCTTCGTGATCCTCCCAGTTCCTCAGATTGTTTATTGGTTGGTTTTACCAAAACTAATCAGAGAAGAGAGAATCAAGCTTATAATGACTGTAATCTTGTTAATGTTCTTGTTCCAATTCCTCCCAAAAGTTTACCACTCCATTATTTTAATGACAAGAATGCAAAAGGTCACTGGATACATCTTTGGCACCATTTGGTGGGGTTTTGGACTCAATCTCATTGCCTATTTCATCGCCTCTCATGTTGCTGGGGGTTGCTGGTATGTTCTTGCAATACAGCGAGTTGCTTCTTGCATCCAGCAACATTGTGAGAGAAACAAGTGCAACCTATCTCTGTCTTGCTCAGAGGAGGTGTGTTATCAGTTTCTCTCATCTTCTACAACCATAGGAAATTCGTGTGGTCGTAATTCGACTTCTGCCTTTAGGAAGCCCATATGCTTGGATGTTAATGGTCCATTCGCCTATGGCATCTACAAGTGGGCTCTTCCAGTCATTTCTAGCAATTCCATTGCTGTCAAAATTCTTTATCCCATCTTTTGGGGCTTAATGACTCTTAGCACGTTTGGCAATGATCTCGAGCCCACGAGTAATTGGCTAGAAGTATGTTTTAGTATTTGCACAGTACTCAGTGGATTGCTGCTTTTTACTCTTTTGATTGGTAACATCCAGGTTCTTTTGCACGCCGTCATGGCAAGGAGGCGAAAAATGCAGCTGAGATGTCGAGATTTAGAGTGGTGGATGAGGAGACGACAATTGCCATCTCGTTTGAGACATCGTGTTCGACACTATGAACACCAGAGATGGGCAGCCATGGGTGGAGAAGATGAGATGGAACTGATCAATGATTTGCCTGAAGGTCTTAGAAGAGACATTAAACGCCATCTTTGTGTTGATCTTATCAGAAAGGTGCCGCTGTTCCAAAACCTGGATGAGCTGATTCTCGACAACATCTGCGACCGAGTCAAGCCACTTGTATTTTCCAAAGATGAAAAGATTATCAGAGAAGGAGATCCTGTTCCAAGAATGCTATTCATAGTGTGTGGACGTGTAAAACGTAACCAAAGCCTGAGCAAGGGCATGACAGCAACAAGTTTGCTAGAATCGGGAGGATTTCTGGGCGACGAGCTGCTATCGTGGTGTCTTCGGCGCCCATTTCTCGAGAGGCTTCCAGCTTCATCAGCTACATTTGTATGCATTGAACCAACAGAAGTATTTGCCCTGAAAGCAGACCATCTGAAGTACATAACCGATCACTTCCGCTACAAATTTGCCAACGAACGACTGAAGAGAACTGCAAGATATTACTCCTCCAACTGGAGAACATGGGCTGCTGTGAACATACAATTTGCTTGGCGTCGCTACAGAAAGAGGATGAGACGTCCAGTGATAACTGTGGTGGAAAATGGAAGCAATGAACGTAGGCTTCTGCAGTATGCTGCAATGTTCATGTCATTCAGGCCACATGACCATCTTGAATAGTAAACTATGAGCTTGCCTACACTTCTTGAATTCTTTCATAAGTCATTCATTGATATCATTTTCATATTATTCGATATCTATAATCTCCAAGGAAAGACATGGACTTGGAAGTTGGAAAATGGGATGTATTTGAGTGTAAAGAGTGAGATATGTTCATCTTTCTCCCTTTCATGTCTCTTCCCTCTCTCTTCCTCCGACATGACAGATCATGTTTCTCAACGAAGATGTGGAGTTGAAGATGAATGTATTGTTAAGAATGTTTTTAGTAAGAGAACGTGTCTAGATTGAGTAATGGCAGTTGTCGTCTCTCTTCCATCAACTCGTGAGCTCAACTAGTTAAGATATATTCCATTTGTTCGTGAATTACAGAAAAAAGTCCTATAGACATTGTGTTTGTTTCATTTGCTAATGGTTAAGATATCTCTTGTAAGATGTAAGAGCTCAGGCACCGCTAGGCTAGCAGATATC

Coding sequence (CDS)

GCAACATTATTTGAAGTGTTGAGAATAATATTGGAGATACGGAAGCATAAAAGTGAAGCCCCCACCACAGCAACACCTCCCCTTACCTTACGTGATTATTCCTCATCTTCTACTTCTTCTTCTTCTGCTGAATTTGCTTCCACTCTCTCCCATGGTGGTTCTGGTTCCTTCATTCTCCCCATGCCTTCTCTCCCTGGCTTCCCCTTCTCCTCTCCCATGTGGAGCGAGCTTTGCCTGAGGCGAACCACCCCTTCACTCAATGCCCACGACAACATTAACAACACCACCACCGCTGCCGATGAACACCCCATCTCCAACGCCGTTGAGTGCTACGCCTGCACCCAAGTCGGTGTTCCGGCCTTCCATTCCACCAGCTGTGACCATGCCCACCAGCAGCCCGAATGGGAGGCCTCCGCCGGCTCTTCCCTCGTCCCCATTCAACCCACAAAGACCTCTCCTCGCCGCGCCGACCCACAACATCGTTCCGGTCGCTTCGGCAGTGTTCTCGACCCTAGAACCAAGCGGGTCCAAAGATGGAACCGGGCTCTGTTATTAGCCCGTGGAATGGCCCTCGCCGTCGATCCTCTCTACTTCTACGCGCTCTCCATCGGACGTGGTGGGTCGCCGTGCCTGTACATGGACGGTGGATTAATCGCCGTTGTTACTGTTCTCCGCACGTTCCTCGATGTCGTGCACTTGTGGCACGTTTGGCTCCAGTTCCGCCTCGCCTATGTGTCCAAGGAGAGCATGGTCATTGGCTGCGGCAAACTCGTCTGGGACGCTCGTGATATCGCCTCCCACTATGTTCGTTCCTTCAAAGGCTTCTGGTTCGATGCCTTCGTGATCCTCCCAGTTCCTCAGATTGTTTATTGGTTGGTTTTACCAAAACTAATCAGAGAAGAGAGAATCAAGCTTATAATGACTGTAATCTTGTTAATGTTCTTGTTCCAATTCCTCCCAAAAGTTTACCACTCCATTATTTTAATGACAAGAATGCAAAAGGTCACTGGATACATCTTTGGCACCATTTGGTGGGGTTTTGGACTCAATCTCATTGCCTATTTCATCGCCTCTCATGTTGCTGGGGGTTGCTGGTATGTTCTTGCAATACAGCGAGTTGCTTCTTGCATCCAGCAACATTGTGAGAGAAACAAGTGCAACCTATCTCTGTCTTGCTCAGAGGAGGTGTGTTATCAGTTTCTCTCATCTTCTACAACCATAGGAAATTCGTGTGGTCGTAATTCGACTTCTGCCTTTAGGAAGCCCATATGCTTGGATGTTAATGGTCCATTCGCCTATGGCATCTACAAGTGGGCTCTTCCAGTCATTTCTAGCAATTCCATTGCTGTCAAAATTCTTTATCCCATCTTTTGGGGCTTAATGACTCTTAGCACGTTTGGCAATGATCTCGAGCCCACGAGTAATTGGCTAGAAGTATGTTTTAGTATTTGCACAGTACTCAGTGGATTGCTGCTTTTTACTCTTTTGATTGGTAACATCCAGGTTCTTTTGCACGCCGTCATGGCAAGGAGGCGAAAAATGCAGCTGAGATGTCGAGATTTAGAGTGGTGGATGAGGAGACGACAATTGCCATCTCGTTTGAGACATCGTGTTCGACACTATGAACACCAGAGATGGGCAGCCATGGGTGGAGAAGATGAGATGGAACTGATCAATGATTTGCCTGAAGGTCTTAGAAGAGACATTAAACGCCATCTTTGTGTTGATCTTATCAGAAAGGTGCCGCTGTTCCAAAACCTGGATGAGCTGATTCTCGACAACATCTGCGACCGAGTCAAGCCACTTGTATTTTCCAAAGATGAAAAGATTATCAGAGAAGGAGATCCTGTTCCAAGAATGCTATTCATAGTGTGTGGACGTGTAAAACGTAACCAAAGCCTGAGCAAGGGCATGACAGCAACAAGTTTGCTAGAATCGGGAGGATTTCTGGGCGACGAGCTGCTATCGTGGTGTCTTCGGCGCCCATTTCTCGAGAGGCTTCCAGCTTCATCAGCTACATTTGTATGCATTGAACCAACAGAAGTATTTGCCCTGAAAGCAGACCATCTGAAGTACATAACCGATCACTTCCGCTACAAATTTGCCAACGAACGACTGAAGAGAACTGCAAGATATTACTCCTCCAACTGGAGAACATGGGCTGCTGTGAACATACAATTTGCTTGGCGTCGCTACAGAAAGAGGATGAGACGTCCAGTGATAACTGTGGTGGAAAATGGAAGCAATGAACGTAGGCTTCTGCAGTATGCTGCAATGTTCATGTCATTCAGGCCACATGACCATCTTGAATAG

Protein sequence

ATLFEVLRIILEIRKHKSEAPTTATPPLTLRDYSSSSTSSSSAEFASTLSHGGSGSFILPMPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQFRLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Homology
BLAST of Cp4.1LG01g22520 vs. ExPASy Swiss-Prot
Match: O65718 (Cyclic nucleotide-gated ion channel 2 OS=Arabidopsis thaliana OX=3702 GN=CNGC2 PE=1 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 1.5e-306
Identity = 538/730 (73.70%), Postives = 601/730 (82.33%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELC---LRRTTPSLNAHDNI-----NNTTTAADEHPISNAVECYA 120
           MPS P F F    W  L     RR T  ++ + N+     +++++ +DE P+ ++VECYA
Sbjct: 1   MPSHPNFIF---RWIGLFSDKFRRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYA 60

Query: 121 CTQVGVPAFHSTSCDHAHQQPEWEASAGSSLVPIQP-TKTSPRRADPQHRSGRFGSVLDP 180
           CTQVGVPAFHSTSCD AH  PEW ASAGSSLVPIQ  +  +P R   +   G FG VLDP
Sbjct: 61  CTQVGVPAFHSTSCDQAH-APEWRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDP 120

Query: 181 RTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSP-CLYMDGGLIAVVTVLRTFLDV 240
           R+KRVQRWNRALLLARGMALAVDPL+FYALSIGR   P CLYMDG   AVVTVLRT LD 
Sbjct: 121 RSKRVQRWNRALLLARGMALAVDPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDA 180

Query: 241 VHLWHVWLQFRLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVY 300
           VHLWHVWLQFRLAYVS+ES+V+GCGKLVWD R IASHY RS  GFWFD  VILPVPQ V+
Sbjct: 181 VHLWHVWLQFRLAYVSRESLVVGCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVF 240

Query: 301 WLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLN 360
           WLV+PKLIREE++KLIMT++LL+FLFQFLPK+YH I LM RMQKVTGYIFGTIWWGF LN
Sbjct: 241 WLVVPKLIREEKVKLIMTILLLIFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALN 300

Query: 361 LIAYFIASHVAGGCWYVLAIQRVASCIQQHCER-NKCNLSLSCSEEVCYQFLSSSTTIGN 420
           LIAYFIASHVAGGCWYVLAIQRVASCI+Q C R   CNLSL+C EEVCYQF+S ++T+G 
Sbjct: 301 LIAYFIASHVAGGCWYVLAIQRVASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGY 360

Query: 421 SC-GRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGN 480
            C   N TS   KP+CLD NGPF YGIY+WALPVISSNS+AVKILYPIFWGLMTLSTF N
Sbjct: 361 PCLSGNLTSVVNKPMCLDSNGPFRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFAN 420

Query: 481 DLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRR 540
           DLEPTSNWLEV FSI  VLSGLLLFTLLIGNIQV LHAVMA++RKMQ+RCRD+EWWM+RR
Sbjct: 421 DLEPTSNWLEVIFSIVMVLSGLLLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRR 480

Query: 541 QLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLD 600
           QLPSRLR RVR +E QRW A+GGEDE+ELI+DLP GLRRDIKR+LC DLI KVPLF+ +D
Sbjct: 481 QLPSRLRQRVRRFERQRWNALGGEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMD 540

Query: 601 ELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGG 660
           +LILDNICDR KP VFSKDEKIIREGDPV RM+FI+ GRVKR QSLSKG+ ATS LE GG
Sbjct: 541 DLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGG 600

Query: 661 FLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKR 720
           +LGDELLSWCLRRPFL+RLP SSATFVC+E  E F+L ++ L+YITDHFRYKFANERLKR
Sbjct: 601 YLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKR 660

Query: 721 TARYYSSNWRTWAAVNIQFAWRRYRKRMR--------RPVITVVENGSNERRLLQYAAMF 771
           TARYYSSNWRTWAAVNIQ AWRR RKR R         PV      G++ERRLLQYAAMF
Sbjct: 661 TARYYSSNWRTWAAVNIQMAWRRRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMF 720

BLAST of Cp4.1LG01g22520 vs. ExPASy Swiss-Prot
Match: Q94AS9 (Cyclic nucleotide-gated ion channel 4 OS=Arabidopsis thaliana OX=3702 GN=CNGC4 PE=2 SV=2)

HSP 1 Score: 599.7 bits (1545), Expect = 4.5e-170
Identity = 309/618 (50.00%), Postives = 420/618 (67.96%), Query Frame = 0

Query: 166 GSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLR 225
           G +LDPR+K V+ WN+  LL     L VDPL+ Y LS+      CL +DG L   VT LR
Sbjct: 77  GRILDPRSKWVREWNKVFLLVCATGLFVDPLFLYTLSVS-DTCMCLLVDGWLALTVTALR 136

Query: 226 TFLDVVHLWHVWLQFRLA----YVSKES-MVIGCGKLVWDARDIASHYVRSFKGFWFDAF 285
           +  D++HLW++W+QF++A    Y   +S      G     +  +A  YV+   GF+FD F
Sbjct: 137 SMTDLLHLWNIWIQFKIARRWPYPGGDSDGDTNKGGGTRGSTRVAPPYVKK-NGFFFDLF 196

Query: 286 VILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIF 345
           VILP+PQ+V W+V+P L++   + L+++V+L+ FLFQ+LPK+YHSI  + R   ++GYIF
Sbjct: 197 VILPLPQVVLWVVIPSLLKRGSVTLVVSVLLVTFLFQYLPKIYHSIRHLRRNATLSGYIF 256

Query: 346 GTIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERN-KCNL-SLSCSEEVCY 405
           GT+WWG  LN+IAYF+A+H AG CWY+L +QR A C+++ CE    C+L  LSC E V Y
Sbjct: 257 GTVWWGIALNMIAYFVAAHAAGACWYLLGVQRSAKCLKEQCENTIGCDLRMLSCKEPVYY 316

Query: 406 QFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFW 465
                           +  A  + +CLD+N  + YG Y+W + ++SS S   KIL+PIFW
Sbjct: 317 GTTVMVLDRARLAWAQNHQA--RSVCLDINTNYTYGAYQWTIQLVSSESRLEKILFPIFW 376

Query: 466 GLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRC 525
           GLMTLSTFGN LE T+ W EV F+I  + SGLLL T+LIGNI+V LHA  ++++ M L+ 
Sbjct: 377 GLMTLSTFGN-LESTTEWSEVVFNIIVLTSGLLLVTMLIGNIKVFLHATTSKKQAMHLKM 436

Query: 526 RDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLI 585
           R++EWWM++R LP   R RVR+YE QRWAAM G DE E++ +LPEGLRRDIK HLC+DL+
Sbjct: 437 RNIEWWMKKRHLPIGFRQRVRNYERQRWAAMRGVDECEMVQNLPEGLRRDIKYHLCLDLV 496

Query: 586 RKVPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGM 645
           R+VPLFQ++D+L+L+NICDRVK L+F+K E I +EGD V RMLF+V G ++ +Q L  G+
Sbjct: 497 RQVPLFQHMDDLVLENICDRVKSLIFTKGETIQKEGDAVQRMLFVVRGHLQSSQLLRDGV 556

Query: 646 TATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFR 705
            +  +L  G F GDELLSWCLRRPF+ERLP SS+T V +E TE F L A+ +KY+T HFR
Sbjct: 557 KSCCMLGPGNFSGDELLSWCLRRPFVERLPPSSSTLVTLETTEAFGLDAEDVKYVTQHFR 616

Query: 706 YKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRM----------RRPVITVVENGS 765
           Y F NE++KR+ARYYS  WRTWAAV +Q AWRRY+ R+          RRP+      G 
Sbjct: 617 YTFVNEKVKRSARYYSPGWRTWAAVAVQLAWRRYKHRLTLTSLSFIRPRRPLSRCASLGE 676

Query: 766 NERRLLQYAAMFMSFRPH 767
           ++ RL  YAA+  S +P+
Sbjct: 677 DKLRL--YAAILTSPKPN 687

BLAST of Cp4.1LG01g22520 vs. ExPASy Swiss-Prot
Match: Q8L7Z0 (Cyclic nucleotide-gated ion channel 17 OS=Arabidopsis thaliana OX=3702 GN=CNGC17 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.8e-102
Identity = 219/592 (36.99%), Postives = 337/592 (56.93%), Query Frame = 0

Query: 165 FGSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGS-PCLYMDGGLIAVVTV 224
           + ++LDP ++ V +WN   +++  +AL +DPLYF+  +IG   + PC   D  L  +VT 
Sbjct: 68  YKTILDPGSEIVLKWNWVFIVSCMVALFIDPLYFFVPAIGGDKNYPCARTDTSLSILVTF 127

Query: 225 LRTFLDVVHLWHVWLQFRLAYVSKES--MVIGCGKLVWDARDIASHYVRSFKGFWFDAFV 284
            RT  D+ +L H++++FR  +++  S   V G G+LV D + IA  Y++S   F  D   
Sbjct: 128 FRTIADLFYLLHIFIKFRTGFIAPNSSTRVFGRGELVMDPKAIAWRYIKS--DFIIDLIA 187

Query: 285 ILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFG 344
            LP+PQIV W V+    +  R       I L+ L Q++P+ Y  I L +++ K TG +  
Sbjct: 188 TLPLPQIVIWFVI-STTKSYRFDHNNNAIALIVLLQYIPRFYLIIPLSSQIVKATGVVTK 247

Query: 345 TIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFL 404
           T W G   NL+ Y +ASHV G  WY+L++ R  SC +  C      ++     ++ Y   
Sbjct: 248 TAWAGAAYNLLLYMLASHVLGAAWYILSVDRYTSCWKSRCNGEAGQVNC----QLYYLDC 307

Query: 405 SSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPV-ISSNSIAVKILYPIFWGL 464
            S          N T  F+  +C   NG F YGI+  A+   + S+    +  Y ++WGL
Sbjct: 308 DSMYDNNQMTWANVTKVFK--LCDARNGEFKYGIFGNAITKNVVSSQFFERYFYCLWWGL 367

Query: 465 MTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRD 524
             LS++G +L  T    E  F++   + GL+LF  LIGN+Q  L ++  R  + +L+ RD
Sbjct: 368 QQLSSYGQNLSTTMFMGETTFAVLIAIFGLVLFAHLIGNMQTYLQSLTVRLEEWRLKKRD 427

Query: 525 LEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRK 584
            E WMR RQLP  LR+RVR YE  +W A  G DE  L+  LP  LRRDI+RHLC+DL+R+
Sbjct: 428 TEEWMRHRQLPEELRNRVRRYEQYKWLATRGVDEEVLLQSLPTDLRRDIQRHLCLDLVRR 487

Query: 585 VPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLS--KGM 644
           VP F  +D+ +LD IC+R+   + ++   ++REGD +  MLFI+ GR++ + +     G 
Sbjct: 488 VPFFSQMDDQLLDAICERLVSSLCTEGTYLVREGDLISEMLFIIRGRLESSTTNGGRTGF 547

Query: 645 TATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFR 704
             + +L  G F G+ELLSW L       LP+S+ T   +   E FAL+A+ LK++ + FR
Sbjct: 548 FNSIILRPGDFCGEELLSWALLPKSTLNLPSSTRTVRALVEVEAFALRAEDLKFVANQFR 607

Query: 705 YKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRMRRPVITVVENGSNE 751
            +  +++L+ T R+YS +WRTWAA  IQ AWRRY++R+    +T +E+  NE
Sbjct: 608 -RLHSKKLQHTFRFYSHHWRTWAACFIQAAWRRYKRRVMENNLTAIESMENE 649

BLAST of Cp4.1LG01g22520 vs. ExPASy Swiss-Prot
Match: Q9SJA4 (Probable cyclic nucleotide-gated ion channel 14 OS=Arabidopsis thaliana OX=3702 GN=CNGC14 PE=2 SV=2)

HSP 1 Score: 373.2 bits (957), Expect = 6.9e-102
Identity = 240/650 (36.92%), Postives = 358/650 (55.08%), Query Frame = 0

Query: 118 VPAFHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRF-----------G 177
           VP F ST+     Q+             + P K+  R   P  R GRF            
Sbjct: 26  VPLFKSTTAPFMKQE-------------VLPKKSKTRLKIP--RFGRFKVFPENFEIERD 85

Query: 178 SVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGR-GGSPCLYMDGGLIAVVTVLR 237
            +LDP    V +WNR  L    +AL VDPL+F+  S+ R G S C+  D  L  V+T  R
Sbjct: 86  KILDPGGDAVLQWNRVFLFWCLVALYVDPLFFFLSSVKRIGRSSCMTTDLKLGIVITFFR 145

Query: 238 TFLDVVHLWHVWLQFRLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPV 297
           T  D+ ++ H+ ++FR AYVS+ S V G G+LV D + IA  Y+RS   F  D    LP+
Sbjct: 146 TLADLFYVLHIVIKFRTAYVSRTSRVFGRGELVKDPKLIARRYLRS--DFIVDLIACLPL 205

Query: 298 PQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWW 357
           PQIV W +LP  IR          ++L+ L Q++P++Y    L   + K TG +  T W 
Sbjct: 206 PQIVSWFILPS-IRSSHSDHTTNALVLIVLVQYIPRLYLIFPLSAEIIKATGVVTTTAWA 265

Query: 358 GFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSST 417
           G   NL+ Y +ASH+ G  WY+L+I+R A+C +  C  +K ++ L C  +    F    T
Sbjct: 266 GAAYNLLQYMLASHILGSAWYLLSIERQATCWKAEC--HKESVPLQCVTD----FFDCGT 325

Query: 418 TIGNSCG--RNSTSAFRKPICLDVNG-PFAYGIYKWALPV-ISSNSIAVKILYPIFWGLM 477
              +     +N+T  F    C   N   F +GI+  AL   + S+    K LY +++GL 
Sbjct: 326 LHRDDRNNWQNTTVVFSN--CDPSNNIQFTFGIFADALTKNVVSSPFLEKYLYCLWFGLQ 385

Query: 478 TLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDL 537
            LS++G +L  +++ LE  F+I   + GL+LF LLIGN+Q  L ++  R  + +L+ RD 
Sbjct: 386 NLSSYGQNLSTSTSVLETMFAILVAIFGLVLFALLIGNMQTYLQSITVRLEEWRLKRRDT 445

Query: 538 EWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKV 597
           E WM  R LP  LR RVR +   +W A  G DE  +++ LP  LRRDI+RHLC+DL+R+V
Sbjct: 446 EEWMGHRLLPQNLRERVRRFVQYKWLATRGVDEETILHSLPADLRRDIQRHLCLDLVRRV 505

Query: 598 PLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLS--KGMT 657
           PLF  +D+ +LD IC+R+   + ++   I+REGDPV  MLFI+ G+++ + +     G  
Sbjct: 506 PLFAQMDDQLLDAICERLASSLSTQGNYIVREGDPVTEMLFIIRGKLESSTTNGGRTGFF 565

Query: 658 ATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRY 717
            +  L  G F G+ELL+W L       LP+S+ T   +E  E FAL+A  LK++ + FR 
Sbjct: 566 NSITLRPGDFCGEELLAWALLPKSTVNLPSSTRTVRALEEVEAFALQAGDLKFVANQFR- 625

Query: 718 KFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRMRRPVITVVENGSN 750
           +  +++L+ T RYYS  WRTWAA  +Q AWRRY+++     +++ E+ S+
Sbjct: 626 RLHSKKLQHTFRYYSHQWRTWAACFVQVAWRRYKRKKLAKSLSLAESFSS 648

BLAST of Cp4.1LG01g22520 vs. ExPASy Swiss-Prot
Match: Q9M0A4 (Putative cyclic nucleotide-gated ion channel 9 OS=Arabidopsis thaliana OX=3702 GN=CNGC9 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.8e-101
Identity = 218/577 (37.78%), Postives = 326/577 (56.50%), Query Frame = 0

Query: 168 VLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTF 227
           + DP+ K +   N+  + +  +A++VDPL+ Y L   +    C+ +D  L  + T LRT 
Sbjct: 103 IFDPQDKFLLLCNKLFVTSCILAVSVDPLFLY-LPFVKDNEKCIGIDRKLAIIATTLRTV 162

Query: 228 LDVVHLWHVWLQFRLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQ 287
           +D  +L+H+ L+FR A+V+  S V G G+LV D   IA  Y++ +  F  D   +LP+PQ
Sbjct: 163 IDAFYLFHMALRFRTAFVAPSSRVFGRGELVIDPAQIAKRYLQQY--FIIDFLSVLPLPQ 222

Query: 288 IVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGF 347
           IV W  L  + +   +      +  + L Q++P+      L + +++  G    T W G 
Sbjct: 223 IVVWRFL-YISKGASVLATKRALRSIILVQYIPRFIRLYPLSSELKRTAGVFAETAWAGA 282

Query: 348 GLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTI 407
              L+ Y +ASH+ G  WY+LA++R   C  + C     N SL C     + F  +    
Sbjct: 283 AYYLLLYMLASHIVGAIWYLLALERYNGCWTKVCS----NSSLDCHRN--FLFCGNEKMD 342

Query: 408 GNSCG---RNSTSAFRKPICLDVNGPFAYGIYKWALP--VISSNSIAVKILYPIFWGLMT 467
           G +     ++S      P+    N PF +GIY  AL   ++SS S   K  + ++WGL  
Sbjct: 343 GYAAWTTIKDSVLQLNCPVNTTDNPPFDFGIYLRALSSGIVSSKSFVSKYFFCLWWGLQN 402

Query: 468 LSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLE 527
           LST G  LE ++   EV FSI   ++GLLLF LLIGN+Q  L ++  R  +M+++ RD E
Sbjct: 403 LSTLGQGLETSTYPGEVIFSIALAIAGLLLFALLIGNMQTYLQSLTIRLEEMRVKRRDSE 462

Query: 528 WWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVP 587
            WM  R LP  LR RVR Y+  +W    G DE  L+ +LP+ LRRDIKRHLC+ L+R+VP
Sbjct: 463 QWMHHRMLPPELRERVRRYDQYKWLETRGVDEENLVQNLPKDLRRDIKRHLCLALVRRVP 522

Query: 588 LFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVK--RNQSLSKGMTA 647
           LF+N+DE +LD IC+R+KP ++++   ++REGDPV  MLFI+ GR++         G   
Sbjct: 523 LFENMDERLLDAICERLKPCLYTESSYLVREGDPVNEMLFIIRGRLESVTTDGGRSGFFN 582

Query: 648 TSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYK 707
            SLL+ G F G+ELL+W L       LP+S+ T   +   E FAL AD LK++   FR +
Sbjct: 583 RSLLKEGDFCGEELLTWALDPKSGSNLPSSTRTAKALTEVEAFALIADELKFVASQFR-R 642

Query: 708 FANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRMR 738
             + +++ T R+YS  WRTWAA+ IQ AWRRY K+ +
Sbjct: 643 LHSRQVQHTFRFYSQQWRTWAAIFIQAAWRRYVKKKK 668

BLAST of Cp4.1LG01g22520 vs. NCBI nr
Match: XP_023541307.1 (cyclic nucleotide-gated ion channel 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1442 bits (3732), Expect = 0.0
Identity = 710/710 (100.00%), Postives = 710/710 (100.00%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. NCBI nr
Match: KAG7033196.1 (Cyclic nucleotide-gated ion channel 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1434 bits (3713), Expect = 0.0
Identity = 706/710 (99.44%), Postives = 707/710 (99.58%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRR TPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRATPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. NCBI nr
Match: XP_022957639.1 (cyclic nucleotide-gated ion channel 2 [Cucurbita moschata])

HSP 1 Score: 1432 bits (3708), Expect = 0.0
Identity = 705/710 (99.30%), Postives = 706/710 (99.44%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRR TPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRATPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCDHAHQQPEWEASAGSSLVPIQP KTSPRRADPQHRSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDHAHQQPEWEASAGSSLVPIQPAKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. NCBI nr
Match: KAG6602524.1 (Cyclic nucleotide-gated ion channel 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1431 bits (3704), Expect = 0.0
Identity = 704/710 (99.15%), Postives = 706/710 (99.44%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRR TPSL+ H+NINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRATPSLDVHENINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCDHAHQQPEWEASAGSSLVPIQP KTSPRRADPQHRSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDHAHQQPEWEASAGSSLVPIQPAKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. NCBI nr
Match: XP_022990924.1 (cyclic nucleotide-gated ion channel 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1420 bits (3676), Expect = 0.0
Identity = 699/710 (98.45%), Postives = 705/710 (99.30%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRRTTPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRTTPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCD+AHQQPEWEASAGSSLVPIQP KTSPRRADPQ RSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDYAHQQPEWEASAGSSLVPIQPAKTSPRRADPQLRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSS+TTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSATTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTA+SL+ESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTASSLIESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVF+LKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFSLKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVEN SNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENRSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. ExPASy TrEMBL
Match: A0A6J1H2J6 (cyclic nucleotide-gated ion channel 2 OS=Cucurbita moschata OX=3662 GN=LOC111459113 PE=4 SV=1)

HSP 1 Score: 1432 bits (3708), Expect = 0.0
Identity = 705/710 (99.30%), Postives = 706/710 (99.44%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRR TPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRATPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCDHAHQQPEWEASAGSSLVPIQP KTSPRRADPQHRSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDHAHQQPEWEASAGSSLVPIQPAKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. ExPASy TrEMBL
Match: A0A6J1JTC9 (cyclic nucleotide-gated ion channel 2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487670 PE=4 SV=1)

HSP 1 Score: 1420 bits (3676), Expect = 0.0
Identity = 699/710 (98.45%), Postives = 705/710 (99.30%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPA 120
           MPSLPGFPFSSPMWSELCLRRTTPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPA
Sbjct: 1   MPSLPGFPFSSPMWSELCLRRTTPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPA 60

Query: 121 FHSTSCDHAHQQPEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWN 180
           FHSTSCD+AHQQPEWEASAGSSLVPIQP KTSPRRADPQ RSGRFGSVLDPRTKRVQRWN
Sbjct: 61  FHSTSCDYAHQQPEWEASAGSSLVPIQPAKTSPRRADPQLRSGRFGSVLDPRTKRVQRWN 120

Query: 181 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQF 240
           RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQF
Sbjct: 121 RALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQF 180

Query: 241 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 300
           RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE
Sbjct: 181 RLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIRE 240

Query: 301 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 360
           ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV
Sbjct: 241 ERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHV 300

Query: 361 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFR 420
           AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSS+TTIGNSCGRNSTSAFR
Sbjct: 301 AGGCWYVLAIQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSATTIGNSCGRNSTSAFR 360

Query: 421 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 480
           KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC
Sbjct: 361 KPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVC 420

Query: 481 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 540
           FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH
Sbjct: 421 FSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRH 480

Query: 541 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 600
           YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK
Sbjct: 481 YEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVK 540

Query: 601 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLR 660
           PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTA+SL+ESGGFLGDELLSWCLR
Sbjct: 541 PLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTASSLIESGGFLGDELLSWCLR 600

Query: 661 RPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 720
           RPFLERLPASSATFVCIEPTEVF+LKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW
Sbjct: 601 RPFLERLPASSATFVCIEPTEVFSLKADHLKYITDHFRYKFANERLKRTARYYSSNWRTW 660

Query: 721 AAVNIQFAWRRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           AAVNIQFAWRRYRKRMRRPVITVVEN SNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 AAVNIQFAWRRYRKRMRRPVITVVENRSNERRLLQYAAMFMSFRPHDHLE 710

BLAST of Cp4.1LG01g22520 vs. ExPASy TrEMBL
Match: A0A6J1JUP9 (cyclic nucleotide-gated ion channel 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487670 PE=4 SV=1)

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 687/698 (98.42%), Postives = 693/698 (99.28%), Query Frame = 0

Query: 73  MWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDHAHQQ 132
           MWSELCLRRTTPSL+ HDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCD+AHQQ
Sbjct: 1   MWSELCLRRTTPSLDVHDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDYAHQQ 60

Query: 133 PEWEASAGSSLVPIQPTKTSPRRADPQHRSGRFGSVLDPRTKRVQRWNRALLLARGMALA 192
           PEWEASAGSSLVPIQP KTSPRRADPQ RSGRFGSVLDPRTKRVQRWNRALLLARGMALA
Sbjct: 61  PEWEASAGSSLVPIQPAKTSPRRADPQLRSGRFGSVLDPRTKRVQRWNRALLLARGMALA 120

Query: 193 VDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQFRLAYVSKESMVI 252
           VDPLYFYALSIGRGGSPCLYMDGGL AVVTVLRTFLDVVHLWHVWLQFRLAYVSKESMVI
Sbjct: 121 VDPLYFYALSIGRGGSPCLYMDGGLTAVVTVLRTFLDVVHLWHVWLQFRLAYVSKESMVI 180

Query: 253 GCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTVILL 312
           GCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTVILL
Sbjct: 181 GCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTVILL 240

Query: 313 MFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLAIQR 372
           MFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLAIQR
Sbjct: 241 MFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLAIQR 300

Query: 373 VASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFA 432
           VASCIQQHCERNKCNLSLSCSEEVCYQFLSS+TTIGNSCGRNSTSAFRKPICLDVNGPFA
Sbjct: 301 VASCIQQHCERNKCNLSLSCSEEVCYQFLSSATTIGNSCGRNSTSAFRKPICLDVNGPFA 360

Query: 433 YGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLL 492
           YGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLL
Sbjct: 361 YGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLL 420

Query: 493 FTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGE 552
           FTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGE
Sbjct: 421 FTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGE 480

Query: 553 DEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEKIIR 612
           DEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEKIIR
Sbjct: 481 DEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEKIIR 540

Query: 613 EGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLRRPFLERLPASSA 672
           EGDPVPRMLFIVCGRVKRNQSLSKGMTA+SL+ESGGFLGDELLSWCLRRPFLERLPASSA
Sbjct: 541 EGDPVPRMLFIVCGRVKRNQSLSKGMTASSLIESGGFLGDELLSWCLRRPFLERLPASSA 600

Query: 673 TFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAWRRY 732
           TFVCIEPTEVF+LKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAWRRY
Sbjct: 601 TFVCIEPTEVFSLKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAWRRY 660

Query: 733 RKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           RKRMRRPVITVVEN SNERRLLQYAAMFMSFRPHDHLE
Sbjct: 661 RKRMRRPVITVVENRSNERRLLQYAAMFMSFRPHDHLE 698

BLAST of Cp4.1LG01g22520 vs. ExPASy TrEMBL
Match: A0A0A0KXJ7 (Cyclic nucleotide-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638350 PE=4 SV=1)

HSP 1 Score: 1286 bits (3327), Expect = 0.0
Identity = 640/701 (91.30%), Postives = 659/701 (94.01%), Query Frame = 0

Query: 71  SPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDHAH 130
           S MWSELCLR + P LN          AAD  P+   +ECYACTQVGVPAFHSTSCDHAH
Sbjct: 3   SSMWSELCLRPSPPPLNV---------AADATPV---LECYACTQVGVPAFHSTSCDHAH 62

Query: 131 QQPEWEASAGSSLVPIQPTKTSPRRADPQHRS-GRFGSVLDPRTKRVQRWNRALLLARGM 190
           QQPEWEASAGSSLVPIQPTK+SP    P+H S G FG+VLDPR K VQRWNR LLLARGM
Sbjct: 63  QQPEWEASAGSSLVPIQPTKSSPA---PRHSSAGCFGTVLDPRKKPVQRWNRVLLLARGM 122

Query: 191 ALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQFRLAYVSKES 250
           +LAVDPLYFYALSIGRGG PCLYMDGGL A VTV+RT LD+VHLWHVWLQFRLAYVSKES
Sbjct: 123 SLAVDPLYFYALSIGRGGWPCLYMDGGLAAGVTVVRTCLDIVHLWHVWLQFRLAYVSKES 182

Query: 251 MVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTV 310
           MVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTV
Sbjct: 183 MVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTV 242

Query: 311 ILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA 370
           ILLMFLFQFLPKVYHSIILM RMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA
Sbjct: 243 ILLMFLFQFLPKVYHSIILMRRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA 302

Query: 371 IQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFRKPICLDVNG 430
           IQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSS TTIG+SCGRNST+ FRKP+CLDVNG
Sbjct: 303 IQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSDTTIGSSCGRNSTATFRKPLCLDVNG 362

Query: 431 PFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG 490
           PFAYGIYKWALPVISSNS+AVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG
Sbjct: 363 PFAYGIYKWALPVISSNSVAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG 422

Query: 491 LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAM 550
           LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRL+HRVRHYEHQRWAAM
Sbjct: 423 LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLKHRVRHYEHQRWAAM 482

Query: 551 GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEK 610
           GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNL+ELILDNICD+VKPLVFSKDEK
Sbjct: 483 GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLEELILDNICDKVKPLVFSKDEK 542

Query: 611 IIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLRRPFLERLPA 670
           IIREGDPVPRMLFIVCGRVKR+QSLSKGMTATS +E GGFLGDELLSWCLRRPFLERLPA
Sbjct: 543 IIREGDPVPRMLFIVCGRVKRSQSLSKGMTATSFIEPGGFLGDELLSWCLRRPFLERLPA 602

Query: 671 SSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAW 730
           SSATFVCIEPTE FALKADHLKYITDHFRYKFANERLKRTAR+YSSNWRTWAAVNIQ AW
Sbjct: 603 SSATFVCIEPTEAFALKADHLKYITDHFRYKFANERLKRTARFYSSNWRTWAAVNIQLAW 662

Query: 731 RRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           RRYRKRMRRPVI VVENGS ERRLLQYAAMFMSFRPHDHLE
Sbjct: 663 RRYRKRMRRPVIAVVENGSTERRLLQYAAMFMSFRPHDHLE 688

BLAST of Cp4.1LG01g22520 vs. ExPASy TrEMBL
Match: A0A1S3CB78 (cyclic nucleotide-gated ion channel 2 OS=Cucumis melo OX=3656 GN=LOC103498518 PE=4 SV=1)

HSP 1 Score: 1275 bits (3300), Expect = 0.0
Identity = 635/701 (90.58%), Postives = 654/701 (93.30%), Query Frame = 0

Query: 71  SPMWSELCLRRTTPSLNAHDNINNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDHAH 130
           S MWSELCLR + P LN          AAD  P+   VECYACTQVGVPAFHSTSCDHAH
Sbjct: 3   SSMWSELCLRPSPPPLNV---------AADATPV---VECYACTQVGVPAFHSTSCDHAH 62

Query: 131 QQPEWEASAGSSLVPIQPTKTSPRRADPQHRS-GRFGSVLDPRTKRVQRWNRALLLARGM 190
           QQPEWEASAGSSLVPIQPTK+SP    P+H S G FG+VLDPR K VQRWNR LLLARGM
Sbjct: 63  QQPEWEASAGSSLVPIQPTKSSP---PPRHSSAGCFGTVLDPRKKPVQRWNRVLLLARGM 122

Query: 191 ALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLRTFLDVVHLWHVWLQFRLAYVSKES 250
           ALAVDPLYFYALSIGRGG PCLYMDGGL   VTV+RT LD+VHLWHVWLQFRLAYVSKES
Sbjct: 123 ALAVDPLYFYALSIGRGGWPCLYMDGGLAGGVTVVRTCLDIVHLWHVWLQFRLAYVSKES 182

Query: 251 MVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTV 310
           MVIGCGKLVW+ARDIASHYVRSFKGFWFDAFVILP+PQIVYWLVLPKLIREERIKLIMTV
Sbjct: 183 MVIGCGKLVWNARDIASHYVRSFKGFWFDAFVILPIPQIVYWLVLPKLIREERIKLIMTV 242

Query: 311 ILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA 370
           ILLMFLFQFLPKVYHSIILM RMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA
Sbjct: 243 ILLMFLFQFLPKVYHSIILMRRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLA 302

Query: 371 IQRVASCIQQHCERNKCNLSLSCSEEVCYQFLSSSTTIGNSCGRNSTSAFRKPICLDVNG 430
           IQRVASCIQQHCERN CNLSLSCSEEVCY+FLSS TTIG+ CGRNST+ FRKP+CLDV G
Sbjct: 303 IQRVASCIQQHCERNNCNLSLSCSEEVCYRFLSSPTTIGSLCGRNSTATFRKPLCLDVKG 362

Query: 431 PFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG 490
           PFAYGIYKWALPVISSNS+AVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG
Sbjct: 363 PFAYGIYKWALPVISSNSVAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSG 422

Query: 491 LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAM 550
           LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRL+HRVRHYEHQRWAAM
Sbjct: 423 LLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLKHRVRHYEHQRWAAM 482

Query: 551 GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEK 610
           GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNL+ELILDNICDRVKPLVFSKDEK
Sbjct: 483 GGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLEELILDNICDRVKPLVFSKDEK 542

Query: 611 IIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLRRPFLERLPA 670
           IIREGDPVPRMLFIVCGRVKR+QSLSKGMTATS +E GGFLGDELLSWCLRRPFLERLPA
Sbjct: 543 IIREGDPVPRMLFIVCGRVKRSQSLSKGMTATSFIEPGGFLGDELLSWCLRRPFLERLPA 602

Query: 671 SSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAW 730
           SSATFVCIEPTE FALKADHLKYITDHFRYKFANERLKRTAR+YSSNWRTWAAVNIQ AW
Sbjct: 603 SSATFVCIEPTEAFALKADHLKYITDHFRYKFANERLKRTARFYSSNWRTWAAVNIQLAW 662

Query: 731 RRYRKRMRRPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 770
           RRYRKRMRRP I VVENGS ERRLLQYAAMFMSFRPHDHLE
Sbjct: 663 RRYRKRMRRPAIAVVENGSTERRLLQYAAMFMSFRPHDHLE 688

BLAST of Cp4.1LG01g22520 vs. TAIR 10
Match: AT5G15410.1 (Cyclic nucleotide-regulated ion channel family protein )

HSP 1 Score: 1053.1 bits (2722), Expect = 1.1e-307
Identity = 538/730 (73.70%), Postives = 601/730 (82.33%), Query Frame = 0

Query: 61  MPSLPGFPFSSPMWSELC---LRRTTPSLNAHDNI-----NNTTTAADEHPISNAVECYA 120
           MPS P F F    W  L     RR T  ++ + N+     +++++ +DE P+ ++VECYA
Sbjct: 1   MPSHPNFIF---RWIGLFSDKFRRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYA 60

Query: 121 CTQVGVPAFHSTSCDHAHQQPEWEASAGSSLVPIQP-TKTSPRRADPQHRSGRFGSVLDP 180
           CTQVGVPAFHSTSCD AH  PEW ASAGSSLVPIQ  +  +P R   +   G FG VLDP
Sbjct: 61  CTQVGVPAFHSTSCDQAH-APEWRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDP 120

Query: 181 RTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSP-CLYMDGGLIAVVTVLRTFLDV 240
           R+KRVQRWNRALLLARGMALAVDPL+FYALSIGR   P CLYMDG   AVVTVLRT LD 
Sbjct: 121 RSKRVQRWNRALLLARGMALAVDPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDA 180

Query: 241 VHLWHVWLQFRLAYVSKESMVIGCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVY 300
           VHLWHVWLQFRLAYVS+ES+V+GCGKLVWD R IASHY RS  GFWFD  VILPVPQ V+
Sbjct: 181 VHLWHVWLQFRLAYVSRESLVVGCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVF 240

Query: 301 WLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLN 360
           WLV+PKLIREE++KLIMT++LL+FLFQFLPK+YH I LM RMQKVTGYIFGTIWWGF LN
Sbjct: 241 WLVVPKLIREEKVKLIMTILLLIFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALN 300

Query: 361 LIAYFIASHVAGGCWYVLAIQRVASCIQQHCER-NKCNLSLSCSEEVCYQFLSSSTTIGN 420
           LIAYFIASHVAGGCWYVLAIQRVASCI+Q C R   CNLSL+C EEVCYQF+S ++T+G 
Sbjct: 301 LIAYFIASHVAGGCWYVLAIQRVASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGY 360

Query: 421 SC-GRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGN 480
            C   N TS   KP+CLD NGPF YGIY+WALPVISSNS+AVKILYPIFWGLMTLSTF N
Sbjct: 361 PCLSGNLTSVVNKPMCLDSNGPFRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFAN 420

Query: 481 DLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRR 540
           DLEPTSNWLEV FSI  VLSGLLLFTLLIGNIQV LHAVMA++RKMQ+RCRD+EWWM+RR
Sbjct: 421 DLEPTSNWLEVIFSIVMVLSGLLLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRR 480

Query: 541 QLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLD 600
           QLPSRLR RVR +E QRW A+GGEDE+ELI+DLP GLRRDIKR+LC DLI KVPLF+ +D
Sbjct: 481 QLPSRLRQRVRRFERQRWNALGGEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMD 540

Query: 601 ELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGG 660
           +LILDNICDR KP VFSKDEKIIREGDPV RM+FI+ GRVKR QSLSKG+ ATS LE GG
Sbjct: 541 DLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGG 600

Query: 661 FLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKR 720
           +LGDELLSWCLRRPFL+RLP SSATFVC+E  E F+L ++ L+YITDHFRYKFANERLKR
Sbjct: 601 YLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKR 660

Query: 721 TARYYSSNWRTWAAVNIQFAWRRYRKRMR--------RPVITVVENGSNERRLLQYAAMF 771
           TARYYSSNWRTWAAVNIQ AWRR RKR R         PV      G++ERRLLQYAAMF
Sbjct: 661 TARYYSSNWRTWAAVNIQMAWRRRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMF 720

BLAST of Cp4.1LG01g22520 vs. TAIR 10
Match: AT5G15410.2 (Cyclic nucleotide-regulated ion channel family protein )

HSP 1 Score: 1049.7 bits (2713), Expect = 1.2e-306
Identity = 530/708 (74.86%), Postives = 593/708 (83.76%), Query Frame = 0

Query: 80  RRTTPSLNAHDNI-----NNTTTAADEHPISNAVECYACTQVGVPAFHSTSCDHAHQQPE 139
           RR T  ++ + N+     +++++ +DE P+ ++VECYACTQVGVPAFHSTSCD AH  PE
Sbjct: 19  RRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYACTQVGVPAFHSTSCDQAH-APE 78

Query: 140 WEASAGSSLVPIQP-TKTSPRRADPQHRSGRFGSVLDPRTKRVQRWNRALLLARGMALAV 199
           W ASAGSSLVPIQ  +  +P R   +   G FG VLDPR+KRVQRWNRALLLARGMALAV
Sbjct: 79  WRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDPRSKRVQRWNRALLLARGMALAV 138

Query: 200 DPLYFYALSIGRGGSP-CLYMDGGLIAVVTVLRTFLDVVHLWHVWLQFRLAYVSKESMVI 259
           DPL+FYALSIGR   P CLYMDG   AVVTVLRT LD VHLWHVWLQFRLAYVS+ES+V+
Sbjct: 139 DPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDAVHLWHVWLQFRLAYVSRESLVV 198

Query: 260 GCGKLVWDARDIASHYVRSFKGFWFDAFVILPVPQIVYWLVLPKLIREERIKLIMTVILL 319
           GCGKLVWD R IASHY RS  GFWFD  VILPVPQ V+WLV+PKLIREE++KLIMT++LL
Sbjct: 199 GCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVFWLVVPKLIREEKVKLIMTILLL 258

Query: 320 MFLFQFLPKVYHSIILMTRMQKVTGYIFGTIWWGFGLNLIAYFIASHVAGGCWYVLAIQR 379
           +FLFQFLPK+YH I LM RMQKVTGYIFGTIWWGF LNLIAYFIASHVAGGCWYVLAIQR
Sbjct: 259 IFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALNLIAYFIASHVAGGCWYVLAIQR 318

Query: 380 VASCIQQHCER-NKCNLSLSCSEEVCYQFLSSSTTIGNSC-GRNSTSAFRKPICLDVNGP 439
           VASCI+Q C R   CNLSL+C EEVCYQF+S ++T+G  C   N TS   KP+CLD NGP
Sbjct: 319 VASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGYPCLSGNLTSVVNKPMCLDSNGP 378

Query: 440 FAYGIYKWALPVISSNSIAVKILYPIFWGLMTLSTFGNDLEPTSNWLEVCFSICTVLSGL 499
           F YGIY+WALPVISSNS+AVKILYPIFWGLMTLSTF NDLEPTSNWLEV FSI  VLSGL
Sbjct: 379 FRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFANDLEPTSNWLEVIFSIVMVLSGL 438

Query: 500 LLFTLLIGNIQVLLHAVMARRRKMQLRCRDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMG 559
           LLFTLLIGNIQV LHAVMA++RKMQ+RCRD+EWWM+RRQLPSRLR RVR +E QRW A+G
Sbjct: 439 LLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRRQLPSRLRQRVRRFERQRWNALG 498

Query: 560 GEDEMELINDLPEGLRRDIKRHLCVDLIRKVPLFQNLDELILDNICDRVKPLVFSKDEKI 619
           GEDE+ELI+DLP GLRRDIKR+LC DLI KVPLF+ +D+LILDNICDR KP VFSKDEKI
Sbjct: 499 GEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMDDLILDNICDRAKPRVFSKDEKI 558

Query: 620 IREGDPVPRMLFIVCGRVKRNQSLSKGMTATSLLESGGFLGDELLSWCLRRPFLERLPAS 679
           IREGDPV RM+FI+ GRVKR QSLSKG+ ATS LE GG+LGDELLSWCLRRPFL+RLP S
Sbjct: 559 IREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPS 618

Query: 680 SATFVCIEPTEVFALKADHLKYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQFAWR 739
           SATFVC+E  E F+L ++ L+YITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQ AWR
Sbjct: 619 SATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQMAWR 678

Query: 740 RYRKRMR--------RPVITVVENGSNERRLLQYAAMFMSFRPHDHLE 771
           R RKR R         PV      G++ERRLLQYAAMFMS RPHDHLE
Sbjct: 679 RRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMFMSIRPHDHLE 725

BLAST of Cp4.1LG01g22520 vs. TAIR 10
Match: AT5G54250.1 (cyclic nucleotide-gated cation channel 4 )

HSP 1 Score: 599.7 bits (1545), Expect = 3.2e-171
Identity = 309/618 (50.00%), Postives = 420/618 (67.96%), Query Frame = 0

Query: 166 GSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLR 225
           G +LDPR+K V+ WN+  LL     L VDPL+ Y LS+      CL +DG L   VT LR
Sbjct: 77  GRILDPRSKWVREWNKVFLLVCATGLFVDPLFLYTLSVS-DTCMCLLVDGWLALTVTALR 136

Query: 226 TFLDVVHLWHVWLQFRLA----YVSKES-MVIGCGKLVWDARDIASHYVRSFKGFWFDAF 285
           +  D++HLW++W+QF++A    Y   +S      G     +  +A  YV+   GF+FD F
Sbjct: 137 SMTDLLHLWNIWIQFKIARRWPYPGGDSDGDTNKGGGTRGSTRVAPPYVKK-NGFFFDLF 196

Query: 286 VILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIF 345
           VILP+PQ+V W+V+P L++   + L+++V+L+ FLFQ+LPK+YHSI  + R   ++GYIF
Sbjct: 197 VILPLPQVVLWVVIPSLLKRGSVTLVVSVLLVTFLFQYLPKIYHSIRHLRRNATLSGYIF 256

Query: 346 GTIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERN-KCNL-SLSCSEEVCY 405
           GT+WWG  LN+IAYF+A+H AG CWY+L +QR A C+++ CE    C+L  LSC E V Y
Sbjct: 257 GTVWWGIALNMIAYFVAAHAAGACWYLLGVQRSAKCLKEQCENTIGCDLRMLSCKEPVYY 316

Query: 406 QFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFW 465
                           +  A  + +CLD+N  + YG Y+W + ++SS S   KIL+PIFW
Sbjct: 317 GTTVMVLDRARLAWAQNHQA--RSVCLDINTNYTYGAYQWTIQLVSSESRLEKILFPIFW 376

Query: 466 GLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRC 525
           GLMTLSTFGN LE T+ W EV F+I  + SGLLL T+LIGNI+V LHA  ++++ M L+ 
Sbjct: 377 GLMTLSTFGN-LESTTEWSEVVFNIIVLTSGLLLVTMLIGNIKVFLHATTSKKQAMHLKM 436

Query: 526 RDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLI 585
           R++EWWM++R LP   R RVR+YE QRWAAM G DE E++ +LPEGLRRDIK HLC+DL+
Sbjct: 437 RNIEWWMKKRHLPIGFRQRVRNYERQRWAAMRGVDECEMVQNLPEGLRRDIKYHLCLDLV 496

Query: 586 RKVPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGM 645
           R+VPLFQ++D+L+L+NICDRVK L+F+K E I +EGD V RMLF+V G ++ +Q L  G+
Sbjct: 497 RQVPLFQHMDDLVLENICDRVKSLIFTKGETIQKEGDAVQRMLFVVRGHLQSSQLLRDGV 556

Query: 646 TATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFR 705
            +  +L  G F GDELLSWCLRRPF+ERLP SS+T V +E TE F L A+ +KY+T HFR
Sbjct: 557 KSCCMLGPGNFSGDELLSWCLRRPFVERLPPSSSTLVTLETTEAFGLDAEDVKYVTQHFR 616

Query: 706 YKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRM----------RRPVITVVENGS 765
           Y F NE++KR+ARYYS  WRTWAAV +Q AWRRY+ R+          RRP+      G 
Sbjct: 617 YTFVNEKVKRSARYYSPGWRTWAAVAVQLAWRRYKHRLTLTSLSFIRPRRPLSRCASLGE 676

Query: 766 NERRLLQYAAMFMSFRPH 767
           ++ RL  YAA+  S +P+
Sbjct: 677 DKLRL--YAAILTSPKPN 687

BLAST of Cp4.1LG01g22520 vs. TAIR 10
Match: AT5G54250.2 (cyclic nucleotide-gated cation channel 4 )

HSP 1 Score: 599.7 bits (1545), Expect = 3.2e-171
Identity = 309/618 (50.00%), Postives = 420/618 (67.96%), Query Frame = 0

Query: 166 GSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLR 225
           G +LDPR+K V+ WN+  LL     L VDPL+ Y LS+      CL +DG L   VT LR
Sbjct: 77  GRILDPRSKWVREWNKVFLLVCATGLFVDPLFLYTLSVS-DTCMCLLVDGWLALTVTALR 136

Query: 226 TFLDVVHLWHVWLQFRLA----YVSKES-MVIGCGKLVWDARDIASHYVRSFKGFWFDAF 285
           +  D++HLW++W+QF++A    Y   +S      G     +  +A  YV+   GF+FD F
Sbjct: 137 SMTDLLHLWNIWIQFKIARRWPYPGGDSDGDTNKGGGTRGSTRVAPPYVKK-NGFFFDLF 196

Query: 286 VILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIF 345
           VILP+PQ+V W+V+P L++   + L+++V+L+ FLFQ+LPK+YHSI  + R   ++GYIF
Sbjct: 197 VILPLPQVVLWVVIPSLLKRGSVTLVVSVLLVTFLFQYLPKIYHSIRHLRRNATLSGYIF 256

Query: 346 GTIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERN-KCNL-SLSCSEEVCY 405
           GT+WWG  LN+IAYF+A+H AG CWY+L +QR A C+++ CE    C+L  LSC E V Y
Sbjct: 257 GTVWWGIALNMIAYFVAAHAAGACWYLLGVQRSAKCLKEQCENTIGCDLRMLSCKEPVYY 316

Query: 406 QFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFW 465
                           +  A  + +CLD+N  + YG Y+W + ++SS S   KIL+PIFW
Sbjct: 317 GTTVMVLDRARLAWAQNHQA--RSVCLDINTNYTYGAYQWTIQLVSSESRLEKILFPIFW 376

Query: 466 GLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRC 525
           GLMTLSTFGN LE T+ W EV F+I  + SGLLL T+LIGNI+V LHA  ++++ M L+ 
Sbjct: 377 GLMTLSTFGN-LESTTEWSEVVFNIIVLTSGLLLVTMLIGNIKVFLHATTSKKQAMHLKM 436

Query: 526 RDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLI 585
           R++EWWM++R LP   R RVR+YE QRWAAM G DE E++ +LPEGLRRDIK HLC+DL+
Sbjct: 437 RNIEWWMKKRHLPIGFRQRVRNYERQRWAAMRGVDECEMVQNLPEGLRRDIKYHLCLDLV 496

Query: 586 RKVPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGM 645
           R+VPLFQ++D+L+L+NICDRVK L+F+K E I +EGD V RMLF+V G ++ +Q L  G+
Sbjct: 497 RQVPLFQHMDDLVLENICDRVKSLIFTKGETIQKEGDAVQRMLFVVRGHLQSSQLLRDGV 556

Query: 646 TATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFR 705
            +  +L  G F GDELLSWCLRRPF+ERLP SS+T V +E TE F L A+ +KY+T HFR
Sbjct: 557 KSCCMLGPGNFSGDELLSWCLRRPFVERLPPSSSTLVTLETTEAFGLDAEDVKYVTQHFR 616

Query: 706 YKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRM----------RRPVITVVENGS 765
           Y F NE++KR+ARYYS  WRTWAAV +Q AWRRY+ R+          RRP+      G 
Sbjct: 617 YTFVNEKVKRSARYYSPGWRTWAAVAVQLAWRRYKHRLTLTSLSFIRPRRPLSRCASLGE 676

Query: 766 NERRLLQYAAMFMSFRPH 767
           ++ RL  YAA+  S +P+
Sbjct: 677 DKLRL--YAAILTSPKPN 687

BLAST of Cp4.1LG01g22520 vs. TAIR 10
Match: AT5G54250.3 (cyclic nucleotide-gated cation channel 4 )

HSP 1 Score: 585.1 bits (1507), Expect = 8.2e-167
Identity = 305/618 (49.35%), Postives = 415/618 (67.15%), Query Frame = 0

Query: 166 GSVLDPRTKRVQRWNRALLLARGMALAVDPLYFYALSIGRGGSPCLYMDGGLIAVVTVLR 225
           G +LDPR+K V+ WN+  LL     L VDPL+ Y LS+      CL +DG L   VT LR
Sbjct: 77  GRILDPRSKWVREWNKVFLLVCATGLFVDPLFLYTLSVS-DTCMCLLVDGWLALTVTALR 136

Query: 226 TFLDVVHLWHVWLQFRLA----YVSKES-MVIGCGKLVWDARDIASHYVRSFKGFWFDAF 285
           +  D++HLW++W+QF++A    Y   +S      G     +  +A  YV+   GF+FD F
Sbjct: 137 SMTDLLHLWNIWIQFKIARRWPYPGGDSDGDTNKGGGTRGSTRVAPPYVKK-NGFFFDLF 196

Query: 286 VILPVPQIVYWLVLPKLIREERIKLIMTVILLMFLFQFLPKVYHSIILMTRMQKVTGYIF 345
           VILP+PQ+V W+V+P L++   + L+++V+L+ FLFQ+LPK+YHSI  + R   ++GYIF
Sbjct: 197 VILPLPQVVLWVVIPSLLKRGSVTLVVSVLLVTFLFQYLPKIYHSIRHLRRNATLSGYIF 256

Query: 346 GTIWWGFGLNLIAYFIASHVAGGCWYVLAIQRVASCIQQHCERN-KCNL-SLSCSEEVCY 405
           GT+WWG  LN+IAYF+A+H AG CWY+L +QR A C+++ CE    C+L  LSC E V Y
Sbjct: 257 GTVWWGIALNMIAYFVAAHAAGACWYLLGVQRSAKCLKEQCENTIGCDLRMLSCKEPVYY 316

Query: 406 QFLSSSTTIGNSCGRNSTSAFRKPICLDVNGPFAYGIYKWALPVISSNSIAVKILYPIFW 465
                           +  A  + +CLD+N  + YG Y+W + ++SS S   KIL+PIFW
Sbjct: 317 GTTVMVLDRARLAWAQNHQA--RSVCLDINTNYTYGAYQWTIQLVSSESRLEKILFPIFW 376

Query: 466 GLMTLSTFGNDLEPTSNWLEVCFSICTVLSGLLLFTLLIGNIQVLLHAVMARRRKMQLRC 525
           GLMTLSTFGN LE T+ W EV F+I  + SGLLL T+LIGNI+V LHA  ++++ M L+ 
Sbjct: 377 GLMTLSTFGN-LESTTEWSEVVFNIIVLTSGLLLVTMLIGNIKVFLHATTSKKQAMHLKM 436

Query: 526 RDLEWWMRRRQLPSRLRHRVRHYEHQRWAAMGGEDEMELINDLPEGLRRDIKRHLCVDLI 585
           R++EWWM++R LP   R RVR+YE QRWAAM G DE E++ +LPEGLRRDIK HLC+DL+
Sbjct: 437 RNIEWWMKKRHLPIGFRQRVRNYERQRWAAMRGVDECEMVQNLPEGLRRDIKYHLCLDLV 496

Query: 586 RKVPLFQNLDELILDNICDRVKPLVFSKDEKIIREGDPVPRMLFIVCGRVKRNQSLSKGM 645
           R     Q++D+L+L+NICDRVK L+F+K E I +EGD V RMLF+V G ++ +Q L  G+
Sbjct: 497 R-----QHMDDLVLENICDRVKSLIFTKGETIQKEGDAVQRMLFVVRGHLQSSQLLRDGV 556

Query: 646 TATSLLESGGFLGDELLSWCLRRPFLERLPASSATFVCIEPTEVFALKADHLKYITDHFR 705
            +  +L  G F GDELLSWCLRRPF+ERLP SS+T V +E TE F L A+ +KY+T HFR
Sbjct: 557 KSCCMLGPGNFSGDELLSWCLRRPFVERLPPSSSTLVTLETTEAFGLDAEDVKYVTQHFR 616

Query: 706 YKFANERLKRTARYYSSNWRTWAAVNIQFAWRRYRKRM----------RRPVITVVENGS 765
           Y F NE++KR+ARYYS  WRTWAAV +Q AWRRY+ R+          RRP+      G 
Sbjct: 617 YTFVNEKVKRSARYYSPGWRTWAAVAVQLAWRRYKHRLTLTSLSFIRPRRPLSRCASLGE 676

Query: 766 NERRLLQYAAMFMSFRPH 767
           ++ RL  YAA+  S +P+
Sbjct: 677 DKLRL--YAAILTSPKPN 682

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O657181.5e-30673.70Cyclic nucleotide-gated ion channel 2 OS=Arabidopsis thaliana OX=3702 GN=CNGC2 P... [more]
Q94AS94.5e-17050.00Cyclic nucleotide-gated ion channel 4 OS=Arabidopsis thaliana OX=3702 GN=CNGC4 P... [more]
Q8L7Z01.8e-10236.99Cyclic nucleotide-gated ion channel 17 OS=Arabidopsis thaliana OX=3702 GN=CNGC17... [more]
Q9SJA46.9e-10236.92Probable cyclic nucleotide-gated ion channel 14 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9M0A45.8e-10137.78Putative cyclic nucleotide-gated ion channel 9 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
XP_023541307.10.0100.00cyclic nucleotide-gated ion channel 2 [Cucurbita pepo subsp. pepo][more]
KAG7033196.10.099.44Cyclic nucleotide-gated ion channel 2 [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022957639.10.099.30cyclic nucleotide-gated ion channel 2 [Cucurbita moschata][more]
KAG6602524.10.099.15Cyclic nucleotide-gated ion channel 2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022990924.10.098.45cyclic nucleotide-gated ion channel 2 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1H2J60.099.30cyclic nucleotide-gated ion channel 2 OS=Cucurbita moschata OX=3662 GN=LOC111459... [more]
A0A6J1JTC90.098.45cyclic nucleotide-gated ion channel 2 isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1JUP90.098.42cyclic nucleotide-gated ion channel 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0KXJ70.091.30Cyclic nucleotide-binding domain-containing protein OS=Cucumis sativus OX=3659 G... [more]
A0A1S3CB780.090.58cyclic nucleotide-gated ion channel 2 OS=Cucumis melo OX=3656 GN=LOC103498518 PE... [more]
Match NameE-valueIdentityDescription
AT5G15410.11.1e-30773.70Cyclic nucleotide-regulated ion channel family protein [more]
AT5G15410.21.2e-30674.86Cyclic nucleotide-regulated ion channel family protein [more]
AT5G54250.13.2e-17150.00cyclic nucleotide-gated cation channel 4 [more]
AT5G54250.23.2e-17150.00cyclic nucleotide-gated cation channel 4 [more]
AT5G54250.38.2e-16749.35cyclic nucleotide-gated cation channel 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 581..699
e-value: 9.6E-29
score: 101.7
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 188..502
e-value: 3.3E-9
score: 36.3
NoneNo IPR availableGENE3D1.10.287.630Helix hairpin bincoord: 509..574
e-value: 2.5E-16
score: 61.7
NoneNo IPR availableGENE3D1.10.287.70coord: 212..507
e-value: 1.0E-6
score: 30.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..153
NoneNo IPR availablePANTHERPTHR45651CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATEDcoord: 109..770
NoneNo IPR availablePANTHERPTHR45651:SF32CYCLIC NUCLEOTIDE-GATED ION CHANNEL 2-LIKEcoord: 109..770
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 171..504
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 583..662
score: 12.6978
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 583..690
e-value: 3.81111E-16
score: 73.129
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 510..697

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g22520.1Cp4.1LG01g22520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0006811 ion transport
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0090307 mitotic spindle assembly
biological_process GO:0007263 nitric oxide mediated signal transduction
biological_process GO:0009626 plant-type hypersensitive response
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:0055085 transmembrane transport
biological_process GO:0070509 calcium ion import
cellular_component GO:0016020 membrane
cellular_component GO:0072686 mitotic spindle
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005880 nuclear microtubule
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0005222 intracellular cAMP-activated cation channel activity
molecular_function GO:0005242 inward rectifier potassium channel activity
molecular_function GO:0008017 microtubule binding
molecular_function GO:0030295 protein kinase activator activity
molecular_function GO:0005216 ion channel activity