Cp4.1LG01g21250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g21250 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 4.6e-24
Identity = 106/352 (30.11%), Postives = 171/352 (48.58%), Query Frame = 0

Query: 27  PQRSFNVNEEYLCALRSRSFVEFFAK---------AQLIVQESPPSTSSSADHY--CRKF 86
           P  SF+  EEY  A R+ S+ +F+A+         A L+ +       +++      R F
Sbjct: 28  PSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLF 87

Query: 87  SETILLQSDQLEANPSILESPLLVML-PELRGLLIDFFNVSAEASNLCHRLLANLKLARS 146
           +E  LL+ DQ  A  + L SP    L P++RGLL  ++  +A AS LC  LL +++  R 
Sbjct: 88  AEH-LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRL 147

Query: 147 NSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDL--DKRDFARIHDNYAAVSRSLN 206
             R    L  ++ K  S   +  +     A   P + L   +     +      + R L+
Sbjct: 148 RYR---PLKHTLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLD 207

Query: 207 ITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYF-RLKSIRRKLLQH 266
             RKK   +IRS+  + R    +  +T+  + A+V A     +L  F     +    L  
Sbjct: 208 AGRKKARHRIRSVARL-RRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGE 267

Query: 267 QMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDK 326
           +        R   QLEAAAKG+YILNR+ +T SRLV R+ D  +H  A++RL VE +   
Sbjct: 268 RFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAA 327

Query: 327 FA------VAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
            A      V   + ++ ++  S R+Q++++EEHL+LC +TIN++R  V+N M
Sbjct: 328 GAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVMNFM 373

BLAST of Cp4.1LG01g21250 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 3.0e-23
Identity = 95/359 (26.46%), Postives = 168/359 (46.80%), Query Frame = 0

Query: 32  NVNEEYLCALRSRSFVEFFAKAQ--------LIVQESPPSTSSSADHYCRKFSETILLQS 91
           +VNEEY  A R+ S++E   KA+          +  S PS SSS+D          LL  
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 92  DQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLL 151
            Q   +  + +S        L  L++ FF++S+EA ++C  LL  L+  + N   +++++
Sbjct: 95  PQETLDALMQDS-------SLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 152 DSIEKC--------CSPKEIETITSDLLAQRAPLSD-----LDKRDFARIHDNYAAVSRS 211
              ++         CSP+ +  +    L++ A L +     +++  F  +HD  + +   
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 212 LNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR---------- 271
           L   ++++ RKIR  K   +     + IT   +   +L      +L  F           
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 272 --LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGK 331
             L+  + K   H+   +  LE++G Q++ AAKG +IL  + DT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 332 AMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
            +  +  + ++ +  +  A+ E          Q++++EEHLYLC  TINRSR  V+ Q+
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Cp4.1LG01g21250 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.0e-23
Identity = 105/352 (29.83%), Postives = 169/352 (48.01%), Query Frame = 0

Query: 27  PQRSFNVNEEYLCALRSRSFVEFFAK---------AQLIVQESPPSTSSSADHY--CRKF 86
           P  SF+  EEY  A R+ S+ +F+A+         A L+ +       +++      R F
Sbjct: 28  PSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLF 87

Query: 87  SETILLQSDQLEANPSILESPLLVML-PELRGLLIDFFNVSAEASNLCHRLLANLKLARS 146
           +E  LL+ DQ  A  + L SP    L P++RGLL  ++  +A AS LC  LL +++  R 
Sbjct: 88  AEH-LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRL 147

Query: 147 NSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDL--DKRDFARIHDNYAAVSRSLN 206
             R    L  ++ K  S   +  +     A   P + L   +     +      + R L+
Sbjct: 148 RYR---PLKHTLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLD 207

Query: 207 ITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYF-RLKSIRRKLLQH 266
             RKK   +IRS+  + R    +  +T+  + A+V A     +L  F     +    L  
Sbjct: 208 AGRKKARHRIRSVARL-RRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGE 267

Query: 267 QMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDK 326
           +        R   QLEAAAKG+YILNR+ +T SRLV R+ D  +H  A+ RL VE +   
Sbjct: 268 RFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAA 327

Query: 327 FA------VAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
            A      V   + ++ ++  S R+Q++++EEHL+LC +T N++R  V+N M
Sbjct: 328 GAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVMNFM 373

BLAST of Cp4.1LG01g21250 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-17
Identity = 97/346 (28.03%), Postives = 160/346 (46.24%), Query Frame = 0

Query: 32  NVNEEYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEANPS 91
           +V EEY  A R+ S+  F+ +   + ++    +SSS+       S +  L S +L A+ +
Sbjct: 70  DVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH-N 129

Query: 92  ILESPLLVMLPEL---------RGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKL 151
           +L+  L  +   L         R LL D+F  +A A  LC +LL N+   RS    ++  
Sbjct: 130 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 189

Query: 152 LDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKI 211
             S E   S   I+  T ++     P      R    I      + + L   R K   K+
Sbjct: 190 FHS-ENHNSLALIDQFT-EISKWFDPFISSGSR-IQLIRSGCLYLLKRLESRRDKTRAKL 249

Query: 212 RSIK--------IIDRYTCGLVAITSRGLTALVLAADQGPVL--RYFRLKSIRRKLLQHQ 271
           + I         ++   T  L+   +    AL LAA   P L    F+   +R K     
Sbjct: 250 KLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAA---PTLLASQFKPAGLRNK----- 309

Query: 272 MLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKF 331
                 L +   +L+ AAKG+YIL+R+ DT SRLV R+ D V+H +AM   +V R   + 
Sbjct: 310 ------LTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRV 369

Query: 332 AVAVAM-DEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
             +  +  E+KR   S  ++++++EEH+YLC +TINR+R  ++ ++
Sbjct: 370 RGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Cp4.1LG01g21250 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 7.1e-17
Identity = 65/268 (24.25%), Postives = 126/268 (47.01%), Query Frame = 0

Query: 107 LLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQR 166
           LLI++F+V+ EA   C  LLA +  AR +   +++LL  ++        + +   +    
Sbjct: 126 LLIEYFDVTEEACEACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALARHVRLDN 185

Query: 167 APLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKIRSIK-----------------IID 226
            PLS     +F  +H   + ++  L   ++++ R  R+++                 I+ 
Sbjct: 186 -PLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVA 245

Query: 227 RYTCGLVAITSRGLTALVLAADQGPVLRYFRLKSIRRKLLQHQMLGNGGLERVGEQLEAA 286
                  A+   G+ A    A      R++  ++  +   +H         R G  L+AA
Sbjct: 246 AVVLAAHALVGIGVAAAAFGATPAGAARWWARRAAEKVSSRH-------YARAGATLDAA 305

Query: 287 AKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIR 346
           A+G+YI+ R+ DT SR+V R  D ++HG+ + R+ +    ++  +     E +     +R
Sbjct: 306 ARGAYIVGRDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLR 365

Query: 347 KQVEDVEEHLYLCIVTINRSRASVINQM 358
            Q+ ++EEH+ LC++TINR+R  V ++M
Sbjct: 366 AQLAELEEHVCLCLITINRTRRLVAHEM 385

BLAST of Cp4.1LG01g21250 vs. NCBI nr
Match: XP_023542867.1 (UPF0496 protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 686 bits (1771), Expect = 1.31e-248
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR
Sbjct: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM
Sbjct: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357

BLAST of Cp4.1LG01g21250 vs. NCBI nr
Match: KAG6602186.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670 bits (1728), Expect = 4.68e-242
Identity = 349/357 (97.76%), Postives = 354/357 (99.16%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSSSAD YCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSSADLYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKIIDR+TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLC VTINRSRASVINQM
Sbjct: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCFVTINRSRASVINQM 357

BLAST of Cp4.1LG01g21250 vs. NCBI nr
Match: XP_022959620.1 (UPF0496 protein 3-like [Cucurbita moschata])

HSP 1 Score: 669 bits (1727), Expect = 6.64e-242
Identity = 349/357 (97.76%), Postives = 355/357 (99.44%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSSSAD YCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSSADLYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKIIDR+TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKE+KFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM
Sbjct: 301 VRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357

BLAST of Cp4.1LG01g21250 vs. NCBI nr
Match: KAG7032870.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 668 bits (1724), Expect = 1.90e-241
Identity = 348/357 (97.48%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSSSAD YCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSSADLYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLL NLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLVNLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKIIDR+TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLC VTINRSRASVINQM
Sbjct: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCFVTINRSRASVINQM 357

BLAST of Cp4.1LG01g21250 vs. NCBI nr
Match: XP_022990396.1 (UPF0496 protein 3-like [Cucurbita maxima])

HSP 1 Score: 657 bits (1694), Expect = 6.84e-237
Identity = 344/357 (96.36%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSS+  H+CRKFSETILLQ DQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSTG-HHCRKFSETILLQIDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKII++ TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIINKCTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM
Sbjct: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 356

BLAST of Cp4.1LG01g21250 vs. ExPASy TrEMBL
Match: A0A6J1H6T3 (UPF0496 protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460642 PE=3 SV=1)

HSP 1 Score: 669 bits (1727), Expect = 3.22e-242
Identity = 349/357 (97.76%), Postives = 355/357 (99.44%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSSSAD YCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSSADLYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKIIDR+TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKE+KFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM
Sbjct: 301 VRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357

BLAST of Cp4.1LG01g21250 vs. ExPASy TrEMBL
Match: A0A6J1JMU4 (UPF0496 protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111487265 PE=3 SV=1)

HSP 1 Score: 657 bits (1694), Expect = 3.31e-237
Identity = 344/357 (96.36%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 1   MWTKFRASRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60
           MWTKFRASRFIWISVFADVKHQIRNKPQ+SFNVNEEYLCALRSRSFVEFFAKAQLIVQES
Sbjct: 1   MWTKFRASRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQES 60

Query: 61  PPSTSSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120
           PPSTSS+  H+CRKFSETILLQ DQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN
Sbjct: 61  PPSTSSTG-HHCRKFSETILLQIDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASN 120

Query: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARI 180
           LCHRLLANLKLARSNSRFVQKLLDSIEKC SPKEIETITSDLLAQRAPLSDLDKRDFARI
Sbjct: 121 LCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARI 180

Query: 181 HDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR 240
           HD+YAAVSRSLNITRKKVARKIRSIKII++ TCGLVAITSRGLTALV+AADQGPVLRYFR
Sbjct: 181 HDDYAAVSRSLNITRKKVARKIRSIKIINKCTCGLVAITSRGLTALVMAADQGPVLRYFR 240

Query: 241 LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300
           LKSIRRKLL+HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM
Sbjct: 241 LKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAM 300

Query: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM
Sbjct: 301 VRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 356

BLAST of Cp4.1LG01g21250 vs. ExPASy TrEMBL
Match: A0A6J1BWZ5 (UPF0496 protein 3-like OS=Momordica charantia OX=3673 GN=LOC111006097 PE=3 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 1.64e-149
Identity = 239/352 (67.90%), Postives = 279/352 (79.26%), Query Frame = 0

Query: 8   SRFIWISVFADVKHQIRNKPQRSFNVNEEYLCALRSRSFVEFFAKAQLIVQES-PPSTSS 67
           S+ + IS FAD K QIRNKPQ+SFNVN+EY+CALR++S+VEFF KAQ +++ES PPSTSS
Sbjct: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91

Query: 68  SADHYCR-KFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127
           S     R K S TILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 92  STGRRRRRKLSGTILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151

Query: 128 LANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARIHDNYA 187
           L +++L RSNSR +QK LDSIE C SP+ IETI S+ LA R P SD DK DFA IHD+Y 
Sbjct: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211

Query: 188 AVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFRLKSIR 247
           AVSR LN TRKKVARKI+SI+II+  +CGLVAIT+R LT L            F  +S R
Sbjct: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELF----------RFPPESFR 271

Query: 248 RKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307
           RKLL++QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331

Query: 308 ERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357
           ERKEDKFAV VAMDE+K+SN+ +R QVE+VEEHLYLCIVTINR+R  VINQ+
Sbjct: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 372

BLAST of Cp4.1LG01g21250 vs. ExPASy TrEMBL
Match: A0A5D3DGL5 (UPF0496 protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00820 PE=3 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 1.26e-147
Identity = 242/369 (65.58%), Postives = 284/369 (76.96%), Query Frame = 0

Query: 1   MWTKFRASRFI----------WISVFADVKHQI-RNKPQRSFNVNEEYLCALRSRSFVEF 60
           MWTKF  S+ I            S FAD +H+I R K +RSFNVNEEYLC LR+ SF EF
Sbjct: 1   MWTKFSPSKIINSTKLSQSILACSTFADQEHEIIRKKHKRSFNVNEEYLCTLRTESFAEF 60

Query: 61  FAKAQLIVQESPPST--SSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGL 120
           F K +  V ESPPST  SSSA   C +FSETILLQ  QLEA PSILES  L+MLPEL+GL
Sbjct: 61  FLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSILESSFLLMLPELKGL 120

Query: 121 LIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRA 180
            +D+FN+SA+AS+LC RLLAN KL RS SR +Q+ LDSIEKC S + +E+I S+LLA R+
Sbjct: 121 FVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSETVESIASNLLALRS 180

Query: 181 PLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALV 240
           P SDL+KRDFA IHD+Y  +S  LN TRKKVARKIRS+KI+D  TCGL AIT+R LT LV
Sbjct: 181 PFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITCGLNAITTRTLTDLV 240

Query: 241 LAADQGPVLRYFRLKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLV 300
            AAD+GP +         RKLL+H+ML NGGLE+VGE+LEAAAKGSYIL RE +TTSRLV
Sbjct: 241 KAADRGPGV-------FGRKLLRHEMLRNGGLEKVGEKLEAAAKGSYILKRELETTSRLV 300

Query: 301 VRLGDAVDHGKAMVRLFVERK-EDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTI 355
           VRLGDAVD+GKAMVRLF  RK EDKF V VAMDE+K++N +IRK+VEDVEEHL LCIV I
Sbjct: 301 VRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKRVEDVEEHLCLCIVAI 360

BLAST of Cp4.1LG01g21250 vs. ExPASy TrEMBL
Match: A0A1S3B3L4 (UPF0496 protein 3-like OS=Cucumis melo OX=3656 GN=LOC103485615 PE=3 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 1.26e-147
Identity = 242/369 (65.58%), Postives = 284/369 (76.96%), Query Frame = 0

Query: 1   MWTKFRASRFI----------WISVFADVKHQI-RNKPQRSFNVNEEYLCALRSRSFVEF 60
           MWTKF  S+ I            S FAD +H+I R K +RSFNVNEEYLC LR+ SF EF
Sbjct: 1   MWTKFSPSKIINSTKLSQSILACSTFADQEHEIIRKKHKRSFNVNEEYLCTLRTESFAEF 60

Query: 61  FAKAQLIVQESPPST--SSSADHYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGL 120
           F K +  V ESPPST  SSSA   C +FSETILLQ  QLEA PSILES  L+MLPEL+GL
Sbjct: 61  FLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSILESSFLLMLPELKGL 120

Query: 121 LIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIETITSDLLAQRA 180
            +D+FN+SA+AS+LC RLLAN KL RS SR +Q+ LDSIEKC S + +E+I S+LLA R+
Sbjct: 121 FVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSETVESIASNLLALRS 180

Query: 181 PLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALV 240
           P SDL+KRDFA IHD+Y  +S  LN TRKKVARKIRS+KI+D  TCGL AIT+R LT LV
Sbjct: 181 PFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITCGLNAITTRTLTDLV 240

Query: 241 LAADQGPVLRYFRLKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLV 300
            AAD+GP +         RKLL+H+ML NGGLE+VGE+LEAAAKGSYIL RE +TTSRLV
Sbjct: 241 KAADRGPGV-------FGRKLLRHEMLRNGGLEKVGEKLEAAAKGSYILKRELETTSRLV 300

Query: 301 VRLGDAVDHGKAMVRLFVERK-EDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTI 355
           VRLGDAVD+GKAMVRLF  RK EDKF V VAMDE+K++N +IRK+VEDVEEHL LCIV I
Sbjct: 301 VRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKRVEDVEEHLCLCIVAI 360

BLAST of Cp4.1LG01g21250 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 110.9 bits (276), Expect = 2.1e-24
Identity = 95/359 (26.46%), Postives = 168/359 (46.80%), Query Frame = 0

Query: 32  NVNEEYLCALRSRSFVEFFAKAQ--------LIVQESPPSTSSSADHYCRKFSETILLQS 91
           +VNEEY  A R+ S++E   KA+          +  S PS SSS+D          LL  
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 92  DQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLL 151
            Q   +  + +S        L  L++ FF++S+EA ++C  LL  L+  + N   +++++
Sbjct: 95  PQETLDALMQDS-------SLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 152 DSIEKC--------CSPKEIETITSDLLAQRAPLSD-----LDKRDFARIHDNYAAVSRS 211
              ++         CSP+ +  +    L++ A L +     +++  F  +HD  + +   
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 212 LNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFR---------- 271
           L   ++++ RKIR  K   +     + IT   +   +L      +L  F           
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 272 --LKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGK 331
             L+  + K   H+   +  LE++G Q++ AAKG +IL  + DT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 332 AMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
            +  +  + ++ +  +  A+ E          Q++++EEHLYLC  TINRSR  V+ Q+
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Cp4.1LG01g21250 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 103.6 bits (257), Expect = 3.4e-22
Identity = 92/347 (26.51%), Postives = 163/347 (46.97%), Query Frame = 0

Query: 32  NVNEEYLCALRSRSFVEFFAKAQ--------LIVQESPPSTSSSADHYCRKFSETILLQS 91
           +VNEEY  A R+ S++E   KA+          +  S PS SSS+D          LL  
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 92  DQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLL 151
            Q   +  + +S        L  L++ FF++S+EA ++C  LL  L+  + N   +++++
Sbjct: 95  PQETLDALMQDS-------SLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 152 DSIEKC--------CSPKEIETITSDLLAQRAPLSD-----LDKRDFARIHDNYAAVSRS 211
              ++         CSP+ +  +    L++ A L +     +++  F  +HD  + +   
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 212 LNITRKKVARKIRSIKIIDRYTCGLVAITSRGLTALVLAADQGPVLRYFRLKSIRRKLLQ 271
           L   ++++ RKI  + +         A    GL +  L            L+  + K   
Sbjct: 215 LTSKKRRIRRKISILGV-------FAAPALLGLCSFCL------------LRKKKAKGRM 274

Query: 272 HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKED 331
           H+   +  LE++G Q++ AAKG +IL  + DT SRL  RL D ++H K +  +  + ++ 
Sbjct: 275 HKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAMCAKSRKI 334

Query: 332 KFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
           +  +  A+ E          Q++++EEHLYLC  TINRSR  V+ Q+
Sbjct: 335 E-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

BLAST of Cp4.1LG01g21250 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-18
Identity = 97/346 (28.03%), Postives = 160/346 (46.24%), Query Frame = 0

Query: 32  NVNEEYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEANPS 91
           +V EEY  A R+ S+  F+ +   + ++    +SSS+       S +  L S +L A+ +
Sbjct: 70  DVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH-N 129

Query: 92  ILESPLLVMLPEL---------RGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKL 151
           +L+  L  +   L         R LL D+F  +A A  LC +LL N+   RS    ++  
Sbjct: 130 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 189

Query: 152 LDSIEKCCSPKEIETITSDLLAQRAPLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKI 211
             S E   S   I+  T ++     P      R    I      + + L   R K   K+
Sbjct: 190 FHS-ENHNSLALIDQFT-EISKWFDPFISSGSR-IQLIRSGCLYLLKRLESRRDKTRAKL 249

Query: 212 RSIK--------IIDRYTCGLVAITSRGLTALVLAADQGPVL--RYFRLKSIRRKLLQHQ 271
           + I         ++   T  L+   +    AL LAA   P L    F+   +R K     
Sbjct: 250 KLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAA---PTLLASQFKPAGLRNK----- 309

Query: 272 MLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKF 331
                 L +   +L+ AAKG+YIL+R+ DT SRLV R+ D V+H +AM   +V R   + 
Sbjct: 310 ------LTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRV 369

Query: 332 AVAVAM-DEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
             +  +  E+KR   S  ++++++EEH+YLC +TINR+R  ++ ++
Sbjct: 370 RGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Cp4.1LG01g21250 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 75.9 bits (185), Expect = 7.5e-14
Identity = 77/347 (22.19%), Postives = 157/347 (45.24%), Query Frame = 0

Query: 30  SFNVNEEYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEAN 89
           +FN++ E   A ++ S+ +  ++  ++V         +  H+     +  LL S  L+ N
Sbjct: 44  TFNLSRELAHAFQTPSYHDVRSRVHVVV-------DLTQIHHRLIQPDIELLLSQVLQPN 103

Query: 90  PSILESPLL-VMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEK 149
              ++  +  V    L  L+  +F  S +A+ LC  L  N+  AR +       L +I  
Sbjct: 104 KECVQEAIRHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFP 163

Query: 150 CCSPKEIETITSDLLAQ--------RAPLSDLDKRDFARIHDNYAAVSRSLNITRKKVAR 209
             S   I+    DL             P S  +   F      ++ +  +L+   +K   
Sbjct: 164 GDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRS 223

Query: 210 KIRSI--KIIDRYTCGLVAITSRGLTALVLAAD--------QGPVLRYFRLKSIRRKLLQ 269
           ++R I         C + A+ +   +A+V+A+          GP+   +   S +RK L 
Sbjct: 224 RVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELT 283

Query: 270 HQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKED 329
           +             QL AA+KG+++LN++ DT  RLV RL   +++ K ++RL +ER  D
Sbjct: 284 NIC-----------QLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRD 343

Query: 330 KFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
             ++   +  +++S++ +  Q++D+E+H+ L    +N++R+ ++ ++
Sbjct: 344 VHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of Cp4.1LG01g21250 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 73.2 bits (178), Expect = 4.9e-13
Identity = 76/331 (22.96%), Postives = 151/331 (45.62%), Query Frame = 0

Query: 30  SFNVNEEYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEAN 89
           +FN++ E   A ++ S+ +  ++  ++V         +  H+     +  LL S  L+ N
Sbjct: 44  TFNLSRELAHAFQTPSYHDVRSRVHVVV-------DLTQIHHRLIQPDIELLLSQVLQPN 103

Query: 90  PSILESPLL-VMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEK 149
              ++  +  V    L  L+  +F  S +A+ LC  L  N+  AR +       L +I  
Sbjct: 104 KECVQEAIRHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFP 163

Query: 150 CCSPKEIETITSDLLAQRAPLSDLDKRDFARIHDNYAAVSRSLNITRKK--VARKIRSIK 209
             S   I+    DL        D  +  F+   ++Y+     L  ++ K  + R++R   
Sbjct: 164 GDSLPAIDESLCDLAFDVFLKLDTFENPFSS-PESYSFRDTQLCFSQLKHNLDRRLRK-- 223

Query: 210 IIDRYTCGLVAITSRGLTALVLAADQGPVLRYFRLKSIRRKLLQHQMLGNGGLERVGEQL 269
                        SR    L+  A  GP+   +   S +RK L +             QL
Sbjct: 224 -------------SRSRVRLIHHATAGPLCSPYLPHSFKRKELTNIC-----------QL 283

Query: 270 EAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNV 329
            AA+KG+++LN++ DT  RLV RL   +++ K ++RL +ER  D  ++   +  +++S++
Sbjct: 284 NAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHL 340

Query: 330 SIRKQVEDVEEHLYLCIVTINRSRASVINQM 358
            +  Q++D+E+H+ L    +N++R+ ++ ++
Sbjct: 344 PLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340