Cp4.1LG01g20880 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g20880
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	wall-associated receptor kinase 3-like
Location	Cp4.1LG01: 17678697 .. 17678939 (-)
RNA-Seq Expression	Cp4.1LG01g20880
Synteny	Cp4.1LG01g20880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGA

mRNA sequence

ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGA

Coding sequence (CDS)

ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGA

Protein sequence

MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNKTDRNGPSRVAASSANLL

Homology

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot
Match: Q9SA25 (Wall-associated receptor kinase-like 8 OS=Arabidopsis thaliana OX=3702 GN=WAKL8 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 5.6e-07
Identity = 25/66 (37.88%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 4  IMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNK 63
          +  F+V+++ + I   A A     AL  C + CG+V +PYPFGI +GCY N+ F I C  
Sbjct: 5  VKRFLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKS 64

Query: 64 TDRNGP 70
          +    P
Sbjct: 65 SSDQQP 70

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot
Match: Q9C9L5 (Wall-associated receptor kinase-like 9 OS=Arabidopsis thaliana OX=3702 GN=WAKL9 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 19/38 (50.00%), Postives = 26/38 (68.42%), Query Frame = 0


Query: 25 ASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCN 63
          A R +  C   CG ++IPYPFG+ +GCYL + + ITCN
Sbjct: 24 ADRTVSLCQPDCGGIKIPYPFGMGKGCYLEKWYEITCN 61

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 3.1e-05
Identity = 24/48 (50.00%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 23 MVASRALPG--CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDR 67
          +V  +  PG  C  +CG++ I YPFGI  GCY   N++FSITC K DR
Sbjct: 21 LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KEDR 67

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot
Match: Q9LMT9 (Putative wall-associated receptor kinase-like 13 OS=Arabidopsis thaliana OX=3702 GN=WAKL13 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 6.9e-05
Identity = 18/38 (47.37%), Postives = 27/38 (71.05%), Query Frame = 0


Query: 32 CDEQCGDVQIPYPFGIKE-GCYLNQNFSITCNKTDRNG 69
          C  +CGD+QIP+PFGI E GCYL++ + + C  +  +G
Sbjct: 26 CTHKCGDIQIPFPFGIGEIGCYLDEWYQVECRPSATSG 63

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot
Match: Q9LN59 (Putative wall-associated receptor kinase-like 11 OS=Arabidopsis thaliana OX=3702 GN=WAKL11 PE=3 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-04
Identity = 16/26 (61.54%), Postives = 22/26 (84.62%), Query Frame = 0


Query: 36 CGDVQIPYPFGIKEGCYLNQNFSITC 62
          CG++ IPYPFGI++GCYLN+ + I C
Sbjct: 38 CGNINIPYPFGIEKGCYLNEWYKIEC 63

[Close]

BLAST of Cp4.1LG01g20880 vs. NCBI nr
Match: KAG6602139.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142 bits (359), Expect = 3.05e-39
Identity = 70/79 (88.61%), Postives = 72/79 (91.14%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKTDRNGPSRVAASSANL 79
          CNKTDRNGP +      N+
Sbjct: 61 CNKTDRNGPPKAYLMQTNI 79

[Close]

BLAST of Cp4.1LG01g20880 vs. NCBI nr
Match: XP_022959563.1 (uncharacterized protein LOC111460594 [Cucurbita moschata])

HSP 1 Score: 133 bits (335), Expect = 4.79e-34
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVMIMEIAILSTALAMVAS+ALP CD++CGDV IPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKTDRNGPSRVAASSANL 79
          CNKTDRNGP +      N+
Sbjct: 61 CNKTDRNGPPKAYLMQTNI 79

[Close]

BLAST of Cp4.1LG01g20880 vs. NCBI nr
Match: XP_023542638.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130 bits (328), Expect = 4.21e-33
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKT 64
          CNKT
Sbjct: 61 CNKT 64

[Close]

BLAST of Cp4.1LG01g20880 vs. NCBI nr
Match: XP_022989771.1 (putative wall-associated receptor kinase-like 16 [Cucurbita maxima])

HSP 1 Score: 129 bits (325), Expect = 9.78e-33
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVM MEIAILSTALAMVAS+ALP CD++CGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVM-MEIAILSTALAMVASQALPRCDDRCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKTDRNGPSRVAASSANL 79
          CNKTDRNGP +      N+
Sbjct: 61 CNKTDRNGPPKAYLMQTNI 78

[Close]

BLAST of Cp4.1LG01g20880 vs. NCBI nr
Match: KAG6602136.1 (Wall-associated receptor kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 114 bits (285), Expect = 2.81e-30
Identity = 56/71 (78.87%), Postives = 63/71 (88.73%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MK IMAF+VM M IAILST LA+ AS+ALPGC+E+CGDV IPYPFGIKEGCYLNQNFSI 
Sbjct: 5  MKPIMAFLVM-MNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQNFSIA 64

Query: 61 CNKTDRNGPSR 71
          C+KTDRNGP +
Sbjct: 65 CDKTDRNGPPK 74

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL
Match: A0A6J1H6B0 (uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC111460594 PE=4 SV=1)

HSP 1 Score: 133 bits (335), Expect = 2.32e-34
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVMIMEIAILSTALAMVAS+ALP CD++CGDV IPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKTDRNGPSRVAASSANL 79
          CNKTDRNGP +      N+
Sbjct: 61 CNKTDRNGPPKAYLMQTNI 79

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL
Match: A0A6J1JL32 (putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=LOC111486859 PE=4 SV=1)

HSP 1 Score: 129 bits (325), Expect = 4.73e-33
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1  MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
          MKHIMAFMVM MEIAILSTALAMVAS+ALP CD++CGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1  MKHIMAFMVM-MEIAILSTALAMVASQALPRCDDRCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61 CNKTDRNGPSRVAASSANL 79
          CNKTDRNGP +      N+
Sbjct: 61 CNKTDRNGPPKAYLMQTNI 78

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL
Match: A0A6J1JGR1 (wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486860 PE=4 SV=1)

HSP 1 Score: 115 bits (287), Expect = 6.79e-28
Identity = 56/73 (76.71%), Postives = 62/73 (84.93%), Query Frame = 0


Query: 2  KHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITC 61
          K IMAFMVM+M+I ILSTALA+ AS ALPGCDE CG V IPYPFGIK+GCYLNQNFSITC
Sbjct: 6  KPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQNFSITC 65

Query: 62 NKTDRNGPSRVAA 74
          +KTD NGP +  A
Sbjct: 66 HKTDLNGPPKAEA 78

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL
Match: A0A6J1H8D6 (uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC111460588 PE=4 SV=1)

HSP 1 Score: 114 bits (285), Expect = 1.31e-27
Identity = 56/71 (78.87%), Postives = 63/71 (88.73%), Query Frame = 0


Query: 1    MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
            MK IMAF+VM M IAILST LA+ AS+ALPGC+E+CGDV IPYPFGIKEGCYLNQNFSI 
Sbjct: 1098 MKPIMAFLVM-MNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQNFSIA 1157

Query: 61   CNKTDRNGPSR 71
            C+KTDRNGP +
Sbjct: 1158 CDKTDRNGPPK 1167

[Close]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL
Match: A0A0A0KDF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.81e-19
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 14 IAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNKT 64
          + ILSTA+ + +S+ALP CDE CGDVQIPYPFG+K+GCYLNQ FSITCNKT
Sbjct: 13 VIILSTAIVVASSQALPDCDEWCGDVQIPYPFGVKQGCYLNQTFSITCNKT 63

[Close]

BLAST of Cp4.1LG01g20880 vs. TAIR 10
Match: AT1G16260.1 (Wall-associated kinase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 4.0e-08
Identity = 25/66 (37.88%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 4  IMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNK 63
          +  F+V+++ + I   A A     AL  C + CG+V +PYPFGI +GCY N+ F I C  
Sbjct: 5  VKRFLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKS 64

Query: 64 TDRNGP 70
          +    P
Sbjct: 65 SSDQQP 70

[Close]

BLAST of Cp4.1LG01g20880 vs. TAIR 10
Match: AT1G16260.2 (Wall-associated kinase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 4.0e-08
Identity = 25/66 (37.88%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 4  IMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNK 63
          +  F+V+++ + I   A A     AL  C + CG+V +PYPFGI +GCY N+ F I C  
Sbjct: 5  VKRFLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKS 64

Query: 64 TDRNGP 70
          +    P
Sbjct: 65 SSDQQP 70

[Close]

BLAST of Cp4.1LG01g20880 vs. TAIR 10
Match: AT1G69730.1 (Wall-associated kinase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.4e-07
Identity = 19/38 (50.00%), Postives = 26/38 (68.42%), Query Frame = 0


Query: 25 ASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCN 63
          A R +  C   CG ++IPYPFG+ +GCYL + + ITCN
Sbjct: 24 ADRTVSLCQPDCGGIKIPYPFGMGKGCYLEKWYEITCN 61

[Close]

BLAST of Cp4.1LG01g20880 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 48.9 bits (115), Expect = 2.2e-06
Identity = 24/48 (50.00%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 23 MVASRALPG--CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDR 67
          +V  +  PG  C  +CG++ I YPFGI  GCY   N++FSITC K DR
Sbjct: 21 LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KEDR 67

[Close]

BLAST of Cp4.1LG01g20880 vs. TAIR 10
Match: AT1G17910.1 (Wall-associated kinase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 4.9e-06
Identity = 18/38 (47.37%), Postives = 27/38 (71.05%), Query Frame = 0


Query: 32 CDEQCGDVQIPYPFGIKE-GCYLNQNFSITCNKTDRNG 69
          C  +CGD+QIP+PFGI E GCYL++ + + C  +  +G
Sbjct: 26 CTHKCGDIQIPFPFGIGEIGCYLDEWYQVECRPSATSG 63

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g20880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA25	5.6e-07	37.88	Wall-associated receptor kinase-like 8 OS=Arabidopsis thaliana OX=3702 GN=WAKL8 ...	[more]
Q9C9L5	4.8e-06	50.00	Wall-associated receptor kinase-like 9 OS=Arabidopsis thaliana OX=3702 GN=WAKL9 ...	[more]
Q39191	3.1e-05	50.00	Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S...	[more]
Q9LMT9	6.9e-05	47.37	Putative wall-associated receptor kinase-like 13 OS=Arabidopsis thaliana OX=3702...	[more]
Q9LN59	1.2e-04	61.54	Putative wall-associated receptor kinase-like 11 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Cp4.1LG01g20880 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6602139.1	3.05e-39	88.61	Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022959563.1	4.79e-34	82.28	uncharacterized protein LOC111460594 [Cucurbita moschata]	[more]
XP_023542638.1	4.21e-33	100.00	LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp....	[more]
XP_022989771.1	9.78e-33	82.28	putative wall-associated receptor kinase-like 16 [Cucurbita maxima]	[more]
KAG6602136.1	2.81e-30	78.87	Wall-associated receptor kinase 1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG01g20880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6B0	2.32e-34	82.28	uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC1114605...	[more]
A0A6J1JL32	4.73e-33	82.28	putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1JGR1	6.79e-28	76.71	wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC1114868...	[more]
A0A6J1H8D6	1.31e-27	78.87	uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC1114605...	[more]
A0A0A0KDF0	7.81e-19	74.51	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1	[more]

BLAST of Cp4.1LG01g20880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G16260.1	4.0e-08	37.88	Wall-associated kinase family protein	[more]
AT1G16260.2	4.0e-08	37.88	Wall-associated kinase family protein	[more]
AT1G69730.1	3.4e-07	50.00	Wall-associated kinase family protein	[more]
AT1G21250.1	2.2e-06	50.00	cell wall-associated kinase	[more]
AT1G17910.1	4.9e-06	47.37	Wall-associated kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025287	Wall-associated receptor kinase, galacturonan-binding domain	PFAM	PF13947	GUB_WAK_bind	coord: 32..65 e-value: 3.8E-9 score: 36.9
None	No IPR available	PANTHER	PTHR33491:SF5	OSJNBA0016N04.9 PROTEIN	coord: 6..70
None	No IPR available	PANTHER	PTHR33491	OSJNBA0016N04.9 PROTEIN	coord: 6..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g20880.1	Cp4.1LG01g20880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007166	cell surface receptor signaling pathway
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030247	polysaccharide binding
molecular_function	GO:0004674	protein serine/threonine kinase activity