Cp4.1LG01g20800 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g20800
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionwall-associated receptor kinase 2-like
LocationCp4.1LG01: 17681591 .. 17685014 (+)
RNA-Seq ExpressionCp4.1LG01g20800
SyntenyCp4.1LG01g20800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCGTCAAATCGAGACGCTTATTCGACTCATGATCGTAAACATACTCATCCTATCGTCACCGGTTTACGCATCTCAAGCCTTACTCGGCTGCCCCGATCGGTGTGGCGACTTGCAGATACCATATCCATTCGGAACAAGGGAAGGGTGTTACCTAAACAAAAATTTCTTGATTACCTGTAACACAACTCATTTCGATCCCCCGAGGCCATTTCTCCGGACCGGCAACATTCAAGTTACGAATATATCCATCTCCGGTGAGCTTCAAATCTTGCACTTTGGAGCCAAAGATTGCTACCCCAAAAACAATAGCGTCGACGCACGTCGGACCTCCGCCACTCTCAACTTATCCACGTTCACAGTTTCCAGTACCAAAAACAAGTTCACTGTTATCGGCTGTGATACATACGCTTTTCTTTCTGGCCAAATCGAGGGGCAAAGCTATAGAACAGCATGCGTGGCGTTGTGTGATAACATCACCACCGTAAGAGATGGCGCCTGCTCAGGTAATGGGTGCTGTCAGCTTGACATCCCTAGCGGCTTAAAGGGTTTGAGATATAGGGTGCGCAGCTTCGACAATCACACCGACGTCTTGAGCTTTAATCCATGTGGGTATGCCTTTGTGACCGAGGAAGACAAGTTTCATTTCTCGGCGGCTTATATTCGTGATTTCCCACAAAGGCAAGTTCCTGTTGTGCTTGATTGGGGTATTAGTAATACCACTTGCTCCACGGCTAACAAAAAAAGTAATTGTGTTTGTGGGCCCAATAGCATGATGGTTAACCCTCTGCCTGATGGATCTGAGTATCGTTGTGGGTGCTTGGATGGGTTTGAGGGTAATCCTTATCTTCCTCGAGGATGTCAAGGTAAAGTGTTTGGGATTGTTAGAATTGTTGGAAATGATAATTGGGGTGTTGATTTGAAGTATTGGTTTGAATTTTGTTGTTGTTTCAGATATTGATGAATGTAGGGATGAAAGGCTGAATGATTGCAAGTTTGAGTGTGTTAACACTGAAGGGAACTATACTTGTAATTGTCCGGAGGGTTTTAAAGGTGATGGACGACGTGGGGGTGAAGGTTGCACTCGAAGCTCCAAGTCTTTTGTTCAAGTTATCATTGGTGAGTTCATTTGATATTTGTGTTGTAATTATGTCTTGTGTTTAAGATTTGGATTAGGGTTAGAAATTTTGTGGGATTTCATATTGGTTCGAGAGGGGAATGAAAGATGTGGAGACCTCTTCGTAACAGATGGGTTTTGAAAACGTTGAGGGTTAGTCCGATAGGGAAAGTCTAAAGAGGATAATTTCTGGTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGCGTGGTATGCCAATGAGGACGTTGGGCCTCCAACGGGGTGAATTGAGAGATCCCACATCTGTTGGAGAGGAGAATAAAGGGTGTGGAGCAGACGCGTTTTAAAAATCTTTGATGGTTAGCTCGATAGGGAAAACCTAAAGAGGACAATATCTAATAGTGGTGAGCTTGGATTGTTACAAATAGTATCAGAACTAGACACCCGGCGGTGTGCCAACAAGAACGCTAGGTTCCCAAAGGGATGGATTGTGAGATCTCACGGGGAATGAAGGGTGTGGAAATCTCTCTGTAGCAGACAAATTTTAGAAACCTTGAGGGGAAACTCGAAAGGGAAAACCCAAAGAAGACAATATCTTCTACCAGTGCGCTTGGGCTGTTACAAATGGTATCAAAGCCAAACACCGAAAAGTATGCTAGCAAGGACGTTGGGCCCCCCAAAGGGGTGGATTATGAAATCCCACATCGGTTGGAGAAGAGAATAAATGGTGTGGAAACCTCTCTCTATCAGACACGTTTTTAGAAACCTTGAAGGGAAATTCAAAAGGGAAAACCCAAAAAAGACAATATATGTTAGTGGTAGGCTTGAGTTGTTACATGTTCGATACGGGATGACTATACATAGTTCTCTCAACCTGCTTTATCCTCACCCTAAACTAGTATCAAGTAAAACACAGTTAACTTTAAAAGGAATGTGCACCTTCTTTTAATCCTTTTAAGTTTTCCTTATCATTCTAAACCTTAGATCACTCATTCGAATATGGTCTCGATTCATTCATGCACCTATTCTAAGAAAGATGTCATATCTATATCTATTCGTTCTATGTGTTTCATCTTCTCCTACCTAGGCTCTATTTTGTCATACAAATCTTTATAATTTATGAAACAAAATGCAGGAGTATCCGTGGGGTTCACAGTCTTAGTAATTGGGAGTACATGGCTATACTTAGGCTACAGAAAATGGAAGCTCATCAAACTAAAAGAGAAGTTCTTCGAGGAAAATGGAGGCTTAATGCTTCAACGACATCTCTCTCAATGGAAATCATCCACAGACATGGTCACAATCTTCACCCAAGAGGAGCTAGACAAGGCCACAAACAAGTACGATGAAAGCGCCGTGATTGGAAAAGGCGGCTACGGCACAGTCTACAAAGGCATCTTACCCGACGGCTCAGTAGTAGCAATCAAAAAATCAAAATTAGTGGACCAATCCCAAACATCCCAATTCATCAATGAAGTCATTGTTCTTTCCCAAATCAACCATCGAAACGTGGTCAAGCTCTTAGGGTGTTGTTTAGAGACCCAAGTCCCATTATTGGTCTACGAGTTCGTCACCAACGGCACCCTCTTCGATCACATCCATGACACTACCAAACACGTCCCACTTTCTTGGGAAGCTCGCTTGAGAATCGCTTCGGAAACAGCCGGGGTCATTTCGTATTTGCATTCATCAGCTTCCACTCCAATCATCCATAGAGATATCAAGACGACCAACATACTTTTAGACGATAATTACAATGCAAAGGTCTCTGATTTCGGTGCTTCCAAATTGGTTACATTGGATCAAACTCAATTATCCACTATGGTTCAAGGGACTCTTGGATATTTGGACCCGGAGTATTTGTTAACGAGTGAGTTGACGGAGAAGAGCGATGTGTATAGCTTTGGAATTGTACTTCTAGAGCTGATAACCGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATTTAGCTATGTATGTTCTATGTGCAATGAAGGAAGATCGGTTGGGGGAGGTTGTGGAGAAGGGAATGGCGAGGGAAGGGGAGTTTGGGCAGATAAAAGAAGTGGGGAAGGTGGCGAGGAAGTGCTTGAGAATTAGTGGGGAGGAGCGGCCGAGCATGAAGGAGGTGGCTATGGAGTTGGAGGGTTTGAGAGTGATGGTTGAACATGAGTGGGTTAATGAAGAAAATTTGGTGGCGGATGGAGCTTCGAATATGTTTGTGGTGAGTGGGAGTACAAATGTTGTGGATGATAGCATGAAAGTTCAGATTTTGCCACTGATTCATGATGGAAGATGA

mRNA sequence

ATGGAGCGTCAAATCGAGACGCTTATTCGACTCATGATCGTAAACATACTCATCCTATCGTCACCGGTTTACGCATCTCAAGCCTTACTCGGCTGCCCCGATCGGTGTGGCGACTTGCAGATACCATATCCATTCGGAACAAGGGAAGGGTGTTACCTAAACAAAAATTTCTTGATTACCTGTAACACAACTCATTTCGATCCCCCGAGGCCATTTCTCCGGACCGGCAACATTCAAGTTACGAATATATCCATCTCCGGTGAGCTTCAAATCTTGCACTTTGGAGCCAAAGATTGCTACCCCAAAAACAATAGCGTCGACGCACGTCGGACCTCCGCCACTCTCAACTTATCCACGTTCACAGTTTCCAGTACCAAAAACAAGTTCACTGTTATCGGCTGTGATACATACGCTTTTCTTTCTGGCCAAATCGAGGGGCAAAGCTATAGAACAGCATGCGTGGCGTTGTGTGATAACATCACCACCGTAAGAGATGGCGCCTGCTCAGGTAATGGGTGCTGTCAGCTTGACATCCCTAGCGGCTTAAAGGGTTTGAGATATAGGGTGCGCAGCTTCGACAATCACACCGACGTCTTGAGCTTTAATCCATGTGGGTATGCCTTTGTGACCGAGGAAGACAAGTTTCATTTCTCGGCGGCTTATATTCGTGATTTCCCACAAAGGCAAGTTCCTGTTGTGCTTGATTGGGGTATTAGTAATACCACTTGCTCCACGGCTAACAAAAAAAGTAATTGTGTTTGTGGGCCCAATAGCATGATGGTTAACCCTCTGCCTGATGGATCTGAGTATCGTTGTGGGTGCTTGGATGGGTTTGAGGGTAATCCTTATCTTCCTCGAGGATGTCAAGATATTGATGAATGTAGGGATGAAAGGCTGAATGATTGCAAGTTTGAGTGTGTTAACACTGAAGGGAACTATACTTGTAATTGTCCGGAGGGTTTTAAAGGTGATGGACGACGTGGGGGTGAAGGTTGCACTCGAAGCTCCAAGTCTTTTGTTCAAGTTATCATTGGAGTATCCGTGGGGTTCACAGTCTTAGTAATTGGGAGTACATGGCTATACTTAGGCTACAGAAAATGGAAGCTCATCAAACTAAAAGAGAAGTTCTTCGAGGAAAATGGAGGCTTAATGCTTCAACGACATCTCTCTCAATGGAAATCATCCACAGACATGGTCACAATCTTCACCCAAGAGGAGCTAGACAAGGCCACAAACAAGTACGATGAAAGCGCCGTGATTGGAAAAGGCGGCTACGGCACAGTCTACAAAGGCATCTTACCCGACGGCTCAGTAGTAGCAATCAAAAAATCAAAATTAGTGGACCAATCCCAAACATCCCAATTCATCAATGAAGTCATTGTTCTTTCCCAAATCAACCATCGAAACGTGGAAGATCGGTTGGGGGAGGTTGTGGAGAAGGGAATGGCGAGGGAAGGGGAGTTTGGGCAGATAAAAGAAGTGGGGAAGGTGGCGAGGAAGTGCTTGAGAATTAGTGGGGAGGAGCGGCCGAGCATGAAGGAGGTGGCTATGGAGTTGGAGGGTTTGAGAGTGATGGTTGAACATGAGTGGGTTAATGAAGAAAATTTGGTGGCGGATGGAGCTTCGAATATGTTTGTGGTGAGTGGGAGTACAAATGTTGTGGATGATAGCATGAAAGTTCAGATTTTGCCACTGATTCATGATGGAAGATGA

Coding sequence (CDS)

ATGGAGCGTCAAATCGAGACGCTTATTCGACTCATGATCGTAAACATACTCATCCTATCGTCACCGGTTTACGCATCTCAAGCCTTACTCGGCTGCCCCGATCGGTGTGGCGACTTGCAGATACCATATCCATTCGGAACAAGGGAAGGGTGTTACCTAAACAAAAATTTCTTGATTACCTGTAACACAACTCATTTCGATCCCCCGAGGCCATTTCTCCGGACCGGCAACATTCAAGTTACGAATATATCCATCTCCGGTGAGCTTCAAATCTTGCACTTTGGAGCCAAAGATTGCTACCCCAAAAACAATAGCGTCGACGCACGTCGGACCTCCGCCACTCTCAACTTATCCACGTTCACAGTTTCCAGTACCAAAAACAAGTTCACTGTTATCGGCTGTGATACATACGCTTTTCTTTCTGGCCAAATCGAGGGGCAAAGCTATAGAACAGCATGCGTGGCGTTGTGTGATAACATCACCACCGTAAGAGATGGCGCCTGCTCAGGTAATGGGTGCTGTCAGCTTGACATCCCTAGCGGCTTAAAGGGTTTGAGATATAGGGTGCGCAGCTTCGACAATCACACCGACGTCTTGAGCTTTAATCCATGTGGGTATGCCTTTGTGACCGAGGAAGACAAGTTTCATTTCTCGGCGGCTTATATTCGTGATTTCCCACAAAGGCAAGTTCCTGTTGTGCTTGATTGGGGTATTAGTAATACCACTTGCTCCACGGCTAACAAAAAAAGTAATTGTGTTTGTGGGCCCAATAGCATGATGGTTAACCCTCTGCCTGATGGATCTGAGTATCGTTGTGGGTGCTTGGATGGGTTTGAGGGTAATCCTTATCTTCCTCGAGGATGTCAAGATATTGATGAATGTAGGGATGAAAGGCTGAATGATTGCAAGTTTGAGTGTGTTAACACTGAAGGGAACTATACTTGTAATTGTCCGGAGGGTTTTAAAGGTGATGGACGACGTGGGGGTGAAGGTTGCACTCGAAGCTCCAAGTCTTTTGTTCAAGTTATCATTGGAGTATCCGTGGGGTTCACAGTCTTAGTAATTGGGAGTACATGGCTATACTTAGGCTACAGAAAATGGAAGCTCATCAAACTAAAAGAGAAGTTCTTCGAGGAAAATGGAGGCTTAATGCTTCAACGACATCTCTCTCAATGGAAATCATCCACAGACATGGTCACAATCTTCACCCAAGAGGAGCTAGACAAGGCCACAAACAAGTACGATGAAAGCGCCGTGATTGGAAAAGGCGGCTACGGCACAGTCTACAAAGGCATCTTACCCGACGGCTCAGTAGTAGCAATCAAAAAATCAAAATTAGTGGACCAATCCCAAACATCCCAATTCATCAATGAAGTCATTGTTCTTTCCCAAATCAACCATCGAAACGTGGAAGATCGGTTGGGGGAGGTTGTGGAGAAGGGAATGGCGAGGGAAGGGGAGTTTGGGCAGATAAAAGAAGTGGGGAAGGTGGCGAGGAAGTGCTTGAGAATTAGTGGGGAGGAGCGGCCGAGCATGAAGGAGGTGGCTATGGAGTTGGAGGGTTTGAGAGTGATGGTTGAACATGAGTGGGTTAATGAAGAAAATTTGGTGGCGGATGGAGCTTCGAATATGTTTGTGGTGAGTGGGAGTACAAATGTTGTGGATGATAGCATGAAAGTTCAGATTTTGCCACTGATTCATGATGGAAGATGA

Protein sequence

MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHEWVNEENLVADGASNMFVVSGSTNVVDDSMKVQILPLIHDGR
Homology
BLAST of Cp4.1LG01g20800 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.0e-98
Identity = 206/485 (42.47%), Postives = 295/485 (60.82%), Query Frame = 0

Query: 11  LMIVNILILS-SPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFD 70
           L +V +  L+ + +   Q    C  RCG++ + YPFGT  GCY   +++F +TCN     
Sbjct: 7   LFVVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCN----- 66

Query: 71  PPRPFLRTGNIQVTNISISGELQILHFGAKDCYP---KNNSVDARRTSATLNLSTFTVSS 130
             +  L  GN+ V N+S+SG+L++    ++ CY    K     A+RT+    L  FT+S 
Sbjct: 67  -EQEKLFFGNMPVINMSLSGQLRVRLVRSRVCYDSQGKQTDYIAQRTT----LGNFTLSE 126

Query: 131 TKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLK 190
             N+FTV+GC++YAFL  +  G + Y T C+++CD+ TT ++G+CSG GCCQ+ +P G  
Sbjct: 127 L-NRFTVVGCNSYAFL--RTSGVEKYSTGCISICDSATT-KNGSCSGEGCCQIPVPRGYS 186

Query: 191 GLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQ----VPVVLDWGIS 250
            +R +  SF NH  V  FNPC YAF+ E+  F F A  + D    +     PVVLDW I 
Sbjct: 187 FVRVKPHSFHNHPTVHLFNPCTYAFLVEDGMFDFHA--LEDLNNLRNVTTFPVVLDWSIG 246

Query: 251 NTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERL 310
           + TC     +   VCG NS   +    G+ Y C CL+GFEGNPYLP GCQDI+EC   R 
Sbjct: 247 DKTCKQVEYRG--VCGGNSTCFDS-TGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRH 306

Query: 311 NDCKFE-CVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSK----SFVQVIIGVSVGFTVLV 370
           N  +   C NT+G++ CNCP G++ D       CTR  +     + Q+ +G ++GF+V++
Sbjct: 307 NCSEHSTCENTKGSFNCNCPSGYRKDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIM 366

Query: 371 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKY 430
           +G + L    +  K  +L++KFFE+NGG ML + +S    S   V IFT++ + +ATN Y
Sbjct: 367 LGISCLQQKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGY 426

Query: 431 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRL 480
            ES ++G+GG GTVYKGILPD S+VAIKK++L ++SQ  QFINEV+VLSQINHRNV   L
Sbjct: 427 HESRILGQGGQGTVYKGILPDNSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVL 469


HSP 2 Score: 50.4 bits (119), Expect = 7.6e-05
Identity = 25/63 (39.68%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 472 DRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHEW 531
           +R  E+++  +  E    +I+E  ++A +C R+ GEERP MKEVA ELE LRV   +++W
Sbjct: 627 NRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVAAELEALRVKTTKYKW 686

Query: 532 VNE 534
            ++
Sbjct: 687 SDQ 689

BLAST of Cp4.1LG01g20800 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 317.0 bits (811), Expect = 4.3e-85
Identity = 184/460 (40.00%), Postives = 261/460 (56.74%), Query Frame = 0

Query: 32  CPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGEL 91
           C ++CG++ I YPFG   GCY   N++F ITC        RP +   +I+V N + SG+L
Sbjct: 32  CQNKCGNITIEYPFGISSGCYYPGNESFSITCK-----EDRPHV-LSDIEVANFNHSGQL 91

Query: 92  QILHFGAKDCYPKNNSVDARRTSATL-NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEGQS 151
           Q+L   +  CY +        +S TL NLS     S  NK T +GC+  + L      Q+
Sbjct: 92  QVLLNRSSTCYDEQGKKTEEDSSFTLENLSL----SANNKLTAVGCNALSLLD-TFGMQN 151

Query: 152 YRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYRVRS--FDNHTDVLSFNPCGY 211
           Y TAC++LCD+     DG C+G GCC++D+ + L    +   S    + T    F+PC Y
Sbjct: 152 YSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTSFHDFSPCTY 211

Query: 212 AFVTEEDKFHFSAA--YIRDFPQRQVPVVLDWGISNTTCSTANKKSNCVCGPNSMMVNPL 271
           AF+ E+DKF+FS+    +      + PV+LDW + N TC      S  +CG NS  ++  
Sbjct: 212 AFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVG--STSICGGNSTCLDST 271

Query: 272 PDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE---RLNDCK--FECVNTEGNYTCNCPE 331
           P    Y C C +GF+GNPYL  GCQD++EC        ++C     C N  G + C C  
Sbjct: 272 PRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQS 331

Query: 332 GFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWKLIKLKEKFFEE 391
           G++ D       C R   ++  +++  ++GF V+++G   +    +  K  KL+E+FFE+
Sbjct: 332 GYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQ 391

Query: 392 NGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGYGTVYKGILPDGSVV 451
           NGG ML + LS    S   V IFT++ + KATN Y ES ++G+GG GTVYKGILPD S+V
Sbjct: 392 NGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPDNSIV 451

Query: 452 AIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVE 480
           AIKK++L D SQ  QFINEV+VLSQINHRNV   LG  +E
Sbjct: 452 AIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLE 474


HSP 2 Score: 54.7 bits (130), Expect = 4.0e-06
Identity = 27/64 (42.19%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E++   +  E    +I+E  ++A +C R+ GEERP MKEVA +LE LRV   +H+
Sbjct: 631 ENRLDEIIGGEVMNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHK 690

Query: 531 WVNE 534
           W ++
Sbjct: 691 WSDQ 694

BLAST of Cp4.1LG01g20800 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.7e-81
Identity = 189/483 (39.13%), Postives = 271/483 (56.11%), Query Frame = 0

Query: 11  LMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDP 70
           LM +   +  + +  +Q    C  RCGD+ I YPFG   GCY   + +F ITC     D 
Sbjct: 8   LMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DK 67

Query: 71  PRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTKNK 130
           P       NI+V N + SG+L+ L   +  CY +  + D       L+  +F   S  NK
Sbjct: 68  PNVL---SNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF---SPNNK 127

Query: 131 FTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYR 190
           FT++GC+ +A LS     Q+Y T C++LCD      +  C+G GCC+ ++   L   R  
Sbjct: 128 FTLVGCNAWALLS-TFGIQNYSTGCMSLCDT-PPPPNSKCNGVGCCRTEVSIPLDSHRIE 187

Query: 191 VR--SFDNHTDVLSFNPCGYAFVTEEDKFHFSAAY-IRDFPQ-RQVPVVLDWGISNTTCS 250
            +   F+N T V  FNPC YAF  E+  F+FS+   ++D     + PV+LDW I N TC 
Sbjct: 188 TQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSIGNQTCE 247

Query: 251 TANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDCK- 310
               ++  +CG NS   +    G  Y C CL GF+GNPYL  GCQDI+EC   R+++C  
Sbjct: 248 QVVGRN--ICGGNSTCFDS-TRGKGYNCKCLQGFDGNPYLSDGCQDINEC-TTRIHNCSD 307

Query: 311 -FECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSK------SFVQVIIGVSVGFTVLVIG 370
              C NT G++ C CP G   D       C  + K       +  V++G ++GF ++++ 
Sbjct: 308 TSTCENTLGSFHCQCPSG--SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLT 367

Query: 371 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDE 430
            +++    R  K  +L+++FFE+NGG ML + LS    S   V IFT+E + +AT+ Y+E
Sbjct: 368 ISYIQQKMRHRKNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNE 427

Query: 431 SAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGE 480
           S ++G+GG GTVYKGIL D S+VAIKK++L D+SQ  QFINEV+VLSQINHRNV   LG 
Sbjct: 428 SRILGQGGQGTVYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGC 473


HSP 2 Score: 57.0 bits (136), Expect = 8.1e-07
Identity = 30/64 (46.88%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E+++  +  E    +I+E  ++A +C RI GEERPSMKEVA ELE LRV   +H+
Sbjct: 630 ENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEERPSMKEVAAELEALRVKTTKHQ 689

Query: 531 WVNE 534
           W ++
Sbjct: 690 WSDQ 693

BLAST of Cp4.1LG01g20800 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 2.5e-80
Identity = 185/468 (39.53%), Postives = 257/468 (54.91%), Query Frame = 0

Query: 32  CPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGEL 91
           C  +CG++ I YPFG   GCY   + NF +TC           L  G IQVTNIS SG +
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVV-----EEKLLLFGIIQVTNISHSGHV 90

Query: 92  QILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTKNKFTVIGCDTYAFLSGQIEGQSY 151
            +L     +CY + N  +       L  S+F++SS  NKFT++GC+  + LS     Q+Y
Sbjct: 91  SVLFERFSECYEQKNETNGTALGYQLG-SSFSLSS-NNKFTLVGCNALSLLS-TFGKQNY 150

Query: 152 RTACVALCDNITTVRDGACSGNGCC---QLDIPSGLKGLRY---RVRSFDN------HTD 211
            T C++LC N     +G C+G GCC      +P      ++   R+R+  N      +T 
Sbjct: 151 STGCLSLC-NSQPEANGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVNNSLDLFNTS 210

Query: 212 VLSFNPCGYAFVTEEDKFHFSAAYIRDFPQ----RQVPVVLDWGISNTTCSTANKKSNCV 271
           V  FNPC YAF+ E+ KF+F ++  +D        + PV LDW I N TC  A   S  +
Sbjct: 211 VYQFNPCTYAFLVEDGKFNFDSS--KDLKNLRNVTRFPVALDWSIGNQTCEQAG--STRI 270

Query: 272 CGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN--DCKFECVNTEG 331
           CG NS   N       Y C C +G++GNPY   GC+DIDEC  +  N  D K  C N +G
Sbjct: 271 CGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPK-TCRNRDG 330

Query: 332 NYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWKLIK 391
            + C CP G+          CTR      ++ + + +G  VL++ +  +    ++ K  K
Sbjct: 331 GFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKYTK 390

Query: 392 LKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGYGTVYKG 451
           L+ +FFE+NGG ML + LS    S     IFT+E + +ATN YDES ++G+GG GTVYKG
Sbjct: 391 LRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVYKG 450

Query: 452 ILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVE 480
           ILPD ++VAIKK++L D  Q  QFI+EV+VLSQINHRNV   LG  +E
Sbjct: 451 ILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLE 480


HSP 2 Score: 56.2 bits (134), Expect = 1.4e-06
Identity = 27/64 (42.19%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E+++  +  E    +I+E  ++A +C R+ GEERP MKEVA +LE LRV   +H+
Sbjct: 637 ENRLHEIIDDQVLNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHK 696

Query: 531 WVNE 534
           W ++
Sbjct: 697 WSDQ 700

BLAST of Cp4.1LG01g20800 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.0e-78
Identity = 182/487 (37.37%), Postives = 271/487 (55.65%), Query Frame = 0

Query: 10  RLMIVNILILS-SPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHF 69
           RL +V I  LS   +   Q L  CP++CG++ + YPFG   GC+   + +F ++C   + 
Sbjct: 5   RLFLVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNEN- 64

Query: 70  DPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTK 129
                 L    ++V  IS S +L++L+  +  CY       A+ T    NL   T+S   
Sbjct: 65  ------LFYKGLEVVEISHSSQLRVLYPASYICYNSKGKF-AKGTYYWSNLGNLTLSG-N 124

Query: 130 NKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLR 189
           N  T +GC++YAF+S     ++    C++ CD ++   +G C+G GCCQ  +P+G   L 
Sbjct: 125 NTITALGCNSYAFVSSNGTRRN-SVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLI 184

Query: 190 YRVRSFDNHTDV--LSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQ---VPVVLDWGISNT 249
            R   FDN T V  +S   C YAF+ E  KF ++A+    + Q +    PVVLDW I   
Sbjct: 185 VRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGE 244

Query: 250 TCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE---R 309
           TC    +K    CG N +  N    G  Y C C  GF+GNPYL  GCQDI+EC       
Sbjct: 245 TCGQVGEKK---CGVNGICSNS-ASGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIH 304

Query: 310 LNDCKFE--CVNTEGNYTCNCPEGFKGDGRRGGEGC-TRSSKSFVQ---VIIGVSVGFTV 369
            ++C  +  C N  G++ CNC   ++ +       C  + +  +V+   +++G ++GF V
Sbjct: 305 KHNCSGDSTCENKLGHFRCNCRSRYELNTTT--NTCKPKGNPEYVEWTTIVLGTTIGFLV 364

Query: 370 LVIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATN 429
           +++  + +    +  K  +L+++FFE+NGG ML + LS    S   V IFT+E + +AT+
Sbjct: 365 ILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATD 424

Query: 430 KYDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVED 480
            YDE+ ++G+GG GTVYKGILPD S+VAIKK++L D SQ  QFINEV+VLSQINHRNV  
Sbjct: 425 GYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVK 475

BLAST of Cp4.1LG01g20800 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 570/735 (77.55%), Postives = 570/735 (77.55%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60
           MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT
Sbjct: 1   MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60

Query: 61  CNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTF 120
           CNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTF
Sbjct: 61  CNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTF 120

Query: 121 TVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS 180
           TVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS
Sbjct: 121 TVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS 180

Query: 181 GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN 240
           GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN
Sbjct: 181 GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN 240

Query: 241 TTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN 300
           TTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN
Sbjct: 241 TTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN 300

Query: 301 DCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWL 360
           DCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWL
Sbjct: 301 DCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWL 360

Query: 361 YLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVI 420
           YLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVI
Sbjct: 361 YLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVI 420

Query: 421 GKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV---------- 480
           GKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV          
Sbjct: 421 GKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLET 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 QVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPIIHRDI 540

Query: 541 ------------------------------------------------------------ 570
                                                                       
Sbjct: 541 KTTNILLDDNYNAKVSDFGASKLVTLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYS 600

BLAST of Cp4.1LG01g20800 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 552/734 (75.20%), Postives = 561/734 (76.43%), Query Frame = 0

Query: 3    RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 62
            RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN
Sbjct: 710  RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 769

Query: 63   TTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTV 122
            TTHF+PPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNS+DARRTSATLNLSTFTV
Sbjct: 770  TTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTV 829

Query: 123  SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL 182
            SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL
Sbjct: 830  SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL 889

Query: 183  KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT 242
            KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT
Sbjct: 890  KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT 949

Query: 243  CSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDC 302
            CSTAN KSNC+CGPNSMMVNPL DGSEYRC CLDGFEGNPYLPRGCQDIDECRDERLNDC
Sbjct: 950  CSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDERLNDC 1009

Query: 303  KFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 362
            KFECVNT+GNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL
Sbjct: 1010 KFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 1069

Query: 363  GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGK 422
            GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTD VTIFTQEELDKATNKYDESAVIGK
Sbjct: 1070 GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESAVIGK 1129

Query: 423  GGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV------------ 482
            GGYGTVYKG LPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV            
Sbjct: 1130 GGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLETQV 1189

Query: 483  ------------------------------------------------------------ 542
                                                                        
Sbjct: 1190 PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPIIHRDIKT 1249

Query: 543  ------------------------------------------------------------ 570
                                                                        
Sbjct: 1250 TNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 1309

BLAST of Cp4.1LG01g20800 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 1052 bits (2721), Expect = 0.0
Identity = 548/737 (74.36%), Postives = 558/737 (75.71%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVYA--SQALLGCPDRCGDLQIPYPFGTREGCYLNKNFL 60
           M RQI+T IRLMI+NILILS  VYA  SQALLGC DRCGDLQIPYPFGTREGCYLNKNFL
Sbjct: 1   MGRQIKTHIRLMIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFL 60

Query: 61  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLS 120
           ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNS+DARRTSATLNLS
Sbjct: 61  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLS 120

Query: 121 TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI 180
           TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI
Sbjct: 121 TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI 180

Query: 181 PSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI 240
           PSGLKGLRYRVRSFDNHTDV SFNPCGYAFVTEEDKFHFSAAYIR+FPQRQVPVVLDWGI
Sbjct: 181 PSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDWGI 240

Query: 241 SNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER 300
           SNTTCSTAN KSNC+CGPNSMMVN LPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER
Sbjct: 241 SNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER 300

Query: 301 LNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST 360
           LNDCKFECVNT+GNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST
Sbjct: 301 LNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST 360

Query: 361 WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESA 420
           WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTD VTIFTQEELDKATNKYDESA
Sbjct: 361 WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESA 420

Query: 421 VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV-------- 480
           VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV        
Sbjct: 421 VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCL 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 ETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTPIIHR 540

Query: 541 ------------------------------------------------------------ 570
                                                                       
Sbjct: 541 DIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDV 600

BLAST of Cp4.1LG01g20800 vs. NCBI nr
Match: XP_022141581.1 (putative wall-associated receptor kinase-like 16 [Momordica charantia])

HSP 1 Score: 765 bits (1975), Expect = 6.53e-270
Identity = 418/751 (55.66%), Postives = 481/751 (64.05%), Query Frame = 0

Query: 6   ETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFL 65
           ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+ F 
Sbjct: 2   ETLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFF 61

Query: 66  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLS 125
           I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN  
Sbjct: 62  ISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLNTE 121

Query: 126 TFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQLD 185
            F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL+
Sbjct: 122 -FAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE 181

Query: 186 IPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWG 245
           IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VLDW 
Sbjct: 182 IPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWA 241

Query: 246 ISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE 305
           ISN TC      +NC+CGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC+D 
Sbjct: 242 ISNNTCENT---TNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG 301

Query: 306 RLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGS 365
           R +DCKFECVNTEGNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG 
Sbjct: 302 R-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGL 361

Query: 366 TWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDES 425
           TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S DMV IFTQEEL+KATNKYDES
Sbjct: 362 TWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDES 421

Query: 426 AVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV------- 485
           AV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNV       
Sbjct: 422 AVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCC 481

Query: 486 ------------------------------------------------------------ 545
                                                                       
Sbjct: 482 LETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPII 541

Query: 546 ------------------------------------------------------------ 570
                                                                       
Sbjct: 542 HRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKS 601

BLAST of Cp4.1LG01g20800 vs. NCBI nr
Match: KAG6602141.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714 bits (1842), Expect = 5.83e-256
Identity = 336/345 (97.39%), Postives = 342/345 (99.13%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60
           MERQIETLIRLMIVNILILSSPVYASQAL GCPDRCGDLQIPYPFGTREGCYLNKNFLIT
Sbjct: 1   MERQIETLIRLMIVNILILSSPVYASQALRGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60

Query: 61  CNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTF 120
           CNTTHF+PPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNS+DARRTSATLNLSTF
Sbjct: 61  CNTTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTF 120

Query: 121 TVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS 180
           TVSSTKNKFTVIGCDTYAFLSG+IEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS
Sbjct: 121 TVSSTKNKFTVIGCDTYAFLSGKIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPS 180

Query: 181 GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN 240
           GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN
Sbjct: 181 GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISN 240

Query: 241 TTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN 300
           TTCSTAN KSNC+CGPNSMMVNPLPDGSEYRC CLDGFEGNPYLPRGCQDIDECRDERLN
Sbjct: 241 TTCSTANNKSNCICGPNSMMVNPLPDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDERLN 300

Query: 301 DCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIG 345
           DCKFECVNT+GNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVI+G
Sbjct: 301 DCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIVG 345

BLAST of Cp4.1LG01g20800 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 552/734 (75.20%), Postives = 561/734 (76.43%), Query Frame = 0

Query: 3    RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 62
            RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN
Sbjct: 710  RQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 769

Query: 63   TTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTV 122
            TTHF+PPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNS+DARRTSATLNLSTFTV
Sbjct: 770  TTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTV 829

Query: 123  SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL 182
            SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL
Sbjct: 830  SSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGL 889

Query: 183  KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT 242
            KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT
Sbjct: 890  KGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTT 949

Query: 243  CSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDC 302
            CSTAN KSNC+CGPNSMMVNPL DGSEYRC CLDGFEGNPYLPRGCQDIDECRDERLNDC
Sbjct: 950  CSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDERLNDC 1009

Query: 303  KFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 362
            KFECVNT+GNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL
Sbjct: 1010 KFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYL 1069

Query: 363  GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGK 422
            GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTD VTIFTQEELDKATNKYDESAVIGK
Sbjct: 1070 GYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESAVIGK 1129

Query: 423  GGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV------------ 482
            GGYGTVYKG LPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV            
Sbjct: 1130 GGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLETQV 1189

Query: 483  ------------------------------------------------------------ 542
                                                                        
Sbjct: 1190 PLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPIIHRDIKT 1249

Query: 543  ------------------------------------------------------------ 570
                                                                        
Sbjct: 1250 TNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 1309

BLAST of Cp4.1LG01g20800 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 1052 bits (2721), Expect = 0.0
Identity = 548/737 (74.36%), Postives = 558/737 (75.71%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVYA--SQALLGCPDRCGDLQIPYPFGTREGCYLNKNFL 60
           M RQI+T IRLMI+NILILS  VYA  SQALLGC DRCGDLQIPYPFGTREGCYLNKNFL
Sbjct: 1   MGRQIKTHIRLMIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFL 60

Query: 61  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLS 120
           ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNS+DARRTSATLNLS
Sbjct: 61  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLS 120

Query: 121 TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI 180
           TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI
Sbjct: 121 TFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDI 180

Query: 181 PSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI 240
           PSGLKGLRYRVRSFDNHTDV SFNPCGYAFVTEEDKFHFSAAYIR+FPQRQVPVVLDWGI
Sbjct: 181 PSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDWGI 240

Query: 241 SNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER 300
           SNTTCSTAN KSNC+CGPNSMMVN LPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER
Sbjct: 241 SNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDER 300

Query: 301 LNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST 360
           LNDCKFECVNT+GNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST
Sbjct: 301 LNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGST 360

Query: 361 WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESA 420
           WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTD VTIFTQEELDKATNKYDESA
Sbjct: 361 WLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESA 420

Query: 421 VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV-------- 480
           VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV        
Sbjct: 421 VIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCL 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 ETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTPIIHR 540

Query: 541 ------------------------------------------------------------ 570
                                                                       
Sbjct: 541 DIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDV 600

BLAST of Cp4.1LG01g20800 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 3.16e-270
Identity = 418/751 (55.66%), Postives = 481/751 (64.05%), Query Frame = 0

Query: 6   ETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFL 65
           ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+ F 
Sbjct: 2   ETLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFF 61

Query: 66  ITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLS 125
           I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN  
Sbjct: 62  ISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLNTE 121

Query: 126 TFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQLD 185
            F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL+
Sbjct: 122 -FAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE 181

Query: 186 IPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWG 245
           IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VLDW 
Sbjct: 182 IPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWA 241

Query: 246 ISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE 305
           ISN TC      +NC+CGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC+D 
Sbjct: 242 ISNNTCENT---TNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG 301

Query: 306 RLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGS 365
           R +DCKFECVNTEGNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG 
Sbjct: 302 R-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGL 361

Query: 366 TWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDES 425
           TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S DMV IFTQEEL+KATNKYDES
Sbjct: 362 TWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDES 421

Query: 426 AVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV------- 485
           AV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNV       
Sbjct: 422 AVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCC 481

Query: 486 ------------------------------------------------------------ 545
                                                                       
Sbjct: 482 LETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPII 541

Query: 546 ------------------------------------------------------------ 570
                                                                       
Sbjct: 542 HRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKS 601

BLAST of Cp4.1LG01g20800 vs. ExPASy TrEMBL
Match: A0A1S3B3B1 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 PE=4 SV=1)

HSP 1 Score: 677 bits (1747), Expect = 2.26e-235
Identity = 378/766 (49.35%), Postives = 454/766 (59.27%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVY-------------ASQALLGCPDRCGDLQIPYPFGT 60
           M R  ETLIRL  V I+++ S  +             ASQAL GC + CGD++IPYPFG 
Sbjct: 1   MGRPTETLIRLTAVKIIVILSAAFVVASATTNATVTVASQALPGCDEWCGDVRIPYPFGM 60

Query: 61  REGCYLN-KNFLITCNTTHFDPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNS 120
           REGCYLN + FL+ C +    P       G   +TNISI +GE+++L+  A+ C+P + +
Sbjct: 61  REGCYLNNETFLLQCLSPAGSPNSLMPVLGPNIITNISIITGEIKLLNRVAQACHPPDTA 120

Query: 121 VDARRTSATLNLSTFTVSSTKNKFTVIGCDTYAFLSGQI-EGQSYRTACVALCDNITTVR 180
            D       + +  +T+S TKNKF VIGCDT+A +SG + +GQS+++ C+ALCDNI TV+
Sbjct: 121 TDVFP-DMVVGIPNYTISHTKNKFVVIGCDTFAIVSGNVVDGQSFQSGCLALCDNIGTVK 180

Query: 181 DGACSGNGCCQLDIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRD 240
           DGACSG+GCCQLD+PSGL  + + V S  NHT+V SFNPCGYAFVTE D F FS+ YIR+
Sbjct: 181 DGACSGSGCCQLDLPSGLDSMVFEVASLTNHTNVSSFNPCGYAFVTERDSFDFSSKYIRN 240

Query: 241 FPQRQVPVVLDWGISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYL 300
           FP++ VPVVLDW ISN TC TA  K+NCVCG NS  V+ L D S YRC CLDGFEGNPYL
Sbjct: 241 FPEKIVPVVLDWAISNDTCVTAKNKTNCVCGKNSTKVDLLDDPSRYRCQCLDGFEGNPYL 300

Query: 301 PRGCQDIDECRDERLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVII 360
           P GCQDIDEC+D+ LNDC+FECVNT G+YTCNCPE FKGDG+R G+GCTR+SKSFVQ+I+
Sbjct: 301 PDGCQDIDECKDDNLNDCRFECVNTIGSYTCNCPEDFKGDGKRQGDGCTRNSKSFVQIIV 360

Query: 361 GVSVGFTVLVIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTD-MVTIFT 420
           GV+VGFTVLVIGS WLYLGY+KWK +KLKEKFFE+NGGLMLQ+HLSQW++STD MV IFT
Sbjct: 361 GVTVGFTVLVIGSAWLYLGYKKWKFLKLKEKFFEKNGGLMLQQHLSQWQASTDHMVRIFT 420

Query: 421 QEELDKATNKYDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLS 480
           +E+LDKATNKYD+SAV+GKGG+GTVYKG+L DGSVVAIKKSKLVDQSQT QFINEVIVLS
Sbjct: 421 KEDLDKATNKYDDSAVVGKGGFGTVYKGVLDDGSVVAIKKSKLVDQSQTDQFINEVIVLS 480

Query: 481 QINHRNV----------------------------------------------------- 540
           QINHRNV                                                     
Sbjct: 481 QINHRNVVKLLGCCLETEVPLLVYEFISNGTLYEYVHDKTNDRNSLSWEARLRIAAETAG 540

Query: 541 ------------------------------------------------------------ 570
                                                                       
Sbjct: 541 VISYLHSSASTPIIHRDIKTTNILLDHNYTAKVSDFGASKLVPMDQTQLSTMVQGTLGYL 600

BLAST of Cp4.1LG01g20800 vs. ExPASy TrEMBL
Match: A0A5D3DKI3 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G001160 PE=4 SV=1)

HSP 1 Score: 617 bits (1592), Expect = 2.77e-212
Identity = 347/746 (46.51%), Postives = 438/746 (58.71%), Query Frame = 0

Query: 1   MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60
           M+ Q E L++L+I+    L++ V  ++ L GC + CG+L+IPYPFG ++ CYL++ F IT
Sbjct: 1   MKIQAEDLMKLIILLSGTLAAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSIT 60

Query: 61  CNTTHFDPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNSVDARRTSATLNLST 120
           CN T + PP+PFL+  NI++TNISI  G+L I  F A+DCY KN   ++  T   L L+ 
Sbjct: 61  CNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESN-TRPFLLLNK 120

Query: 121 FTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITT--VRDGACSGNGCCQLD 180
           F +S+T NKF VIGCDTYA++ G+IEG+SY++ C+ALC N  T  ++DG+CSGNGCCQL 
Sbjct: 121 FRISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQ 180

Query: 181 IPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWG 240
           IP GLK L   VRSFDNH++VL FNPCGYAFV ++DKF FS  YI +F Q +VP+VLDWG
Sbjct: 181 IPKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWG 240

Query: 241 I-SNTTCSTANKKSNC-VCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECR 300
           I +NT+CS    K NC +CG N+  +  L DGSEYRC CL+GFEGNPYLP+GCQDIDEC+
Sbjct: 241 IPTNTSCSKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECK 300

Query: 301 DERLNDCKFE--CVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVL 360
           +   + CK++  C NT GNYTC+CP+  KGDG+ GGEGCT +  S + +IIG SVG  VL
Sbjct: 301 NGS-HKCKYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVL 360

Query: 361 VIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNK 420
           VIG  WLYLGYR+WK I+ KEKFF+ NGGLMLQ+HLSQW+S  DM+ IFT+EEL+KATNK
Sbjct: 361 VIGGIWLYLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSP-DMLKIFTREELEKATNK 420

Query: 421 YDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV--- 480
           YDESAV+GKGGYGTVYKG+L DGS VAIKKSKLV+QSQT+QFINEVI+LSQINHRNV   
Sbjct: 421 YDESAVVGKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKL 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 IGCCLETKVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSAST 540

Query: 541 ------------------------------------------------------------ 570
                                                                       
Sbjct: 541 PIIHRDIKSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSELT 600

BLAST of Cp4.1LG01g20800 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 361.3 bits (926), Expect = 1.4e-99
Identity = 206/485 (42.47%), Postives = 295/485 (60.82%), Query Frame = 0

Query: 11  LMIVNILILS-SPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFD 70
           L +V +  L+ + +   Q    C  RCG++ + YPFGT  GCY   +++F +TCN     
Sbjct: 7   LFVVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCN----- 66

Query: 71  PPRPFLRTGNIQVTNISISGELQILHFGAKDCYP---KNNSVDARRTSATLNLSTFTVSS 130
             +  L  GN+ V N+S+SG+L++    ++ CY    K     A+RT+    L  FT+S 
Sbjct: 67  -EQEKLFFGNMPVINMSLSGQLRVRLVRSRVCYDSQGKQTDYIAQRTT----LGNFTLSE 126

Query: 131 TKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLK 190
             N+FTV+GC++YAFL  +  G + Y T C+++CD+ TT ++G+CSG GCCQ+ +P G  
Sbjct: 127 L-NRFTVVGCNSYAFL--RTSGVEKYSTGCISICDSATT-KNGSCSGEGCCQIPVPRGYS 186

Query: 191 GLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQ----VPVVLDWGIS 250
            +R +  SF NH  V  FNPC YAF+ E+  F F A  + D    +     PVVLDW I 
Sbjct: 187 FVRVKPHSFHNHPTVHLFNPCTYAFLVEDGMFDFHA--LEDLNNLRNVTTFPVVLDWSIG 246

Query: 251 NTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERL 310
           + TC     +   VCG NS   +    G+ Y C CL+GFEGNPYLP GCQDI+EC   R 
Sbjct: 247 DKTCKQVEYRG--VCGGNSTCFDS-TGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRH 306

Query: 311 NDCKFE-CVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSK----SFVQVIIGVSVGFTVLV 370
           N  +   C NT+G++ CNCP G++ D       CTR  +     + Q+ +G ++GF+V++
Sbjct: 307 NCSEHSTCENTKGSFNCNCPSGYRKDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIM 366

Query: 371 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKY 430
           +G + L    +  K  +L++KFFE+NGG ML + +S    S   V IFT++ + +ATN Y
Sbjct: 367 LGISCLQQKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGY 426

Query: 431 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRL 480
            ES ++G+GG GTVYKGILPD S+VAIKK++L ++SQ  QFINEV+VLSQINHRNV   L
Sbjct: 427 HESRILGQGGQGTVYKGILPDNSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVL 469


HSP 2 Score: 50.4 bits (119), Expect = 5.4e-06
Identity = 25/63 (39.68%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 472 DRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHEW 531
           +R  E+++  +  E    +I+E  ++A +C R+ GEERP MKEVA ELE LRV   +++W
Sbjct: 627 NRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVAAELEALRVKTTKYKW 686

Query: 532 VNE 534
            ++
Sbjct: 687 SDQ 689

BLAST of Cp4.1LG01g20800 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 317.0 bits (811), Expect = 3.1e-86
Identity = 184/460 (40.00%), Postives = 261/460 (56.74%), Query Frame = 0

Query: 32  CPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGEL 91
           C ++CG++ I YPFG   GCY   N++F ITC        RP +   +I+V N + SG+L
Sbjct: 32  CQNKCGNITIEYPFGISSGCYYPGNESFSITCK-----EDRPHV-LSDIEVANFNHSGQL 91

Query: 92  QILHFGAKDCYPKNNSVDARRTSATL-NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEGQS 151
           Q+L   +  CY +        +S TL NLS     S  NK T +GC+  + L      Q+
Sbjct: 92  QVLLNRSSTCYDEQGKKTEEDSSFTLENLSL----SANNKLTAVGCNALSLLD-TFGMQN 151

Query: 152 YRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYRVRS--FDNHTDVLSFNPCGY 211
           Y TAC++LCD+     DG C+G GCC++D+ + L    +   S    + T    F+PC Y
Sbjct: 152 YSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTSFHDFSPCTY 211

Query: 212 AFVTEEDKFHFSAA--YIRDFPQRQVPVVLDWGISNTTCSTANKKSNCVCGPNSMMVNPL 271
           AF+ E+DKF+FS+    +      + PV+LDW + N TC      S  +CG NS  ++  
Sbjct: 212 AFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVG--STSICGGNSTCLDST 271

Query: 272 PDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE---RLNDCK--FECVNTEGNYTCNCPE 331
           P    Y C C +GF+GNPYL  GCQD++EC        ++C     C N  G + C C  
Sbjct: 272 PRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQS 331

Query: 332 GFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWKLIKLKEKFFEE 391
           G++ D       C R   ++  +++  ++GF V+++G   +    +  K  KL+E+FFE+
Sbjct: 332 GYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQ 391

Query: 392 NGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGYGTVYKGILPDGSVV 451
           NGG ML + LS    S   V IFT++ + KATN Y ES ++G+GG GTVYKGILPD S+V
Sbjct: 392 NGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPDNSIV 451

Query: 452 AIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVE 480
           AIKK++L D SQ  QFINEV+VLSQINHRNV   LG  +E
Sbjct: 452 AIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLE 474


HSP 2 Score: 54.7 bits (130), Expect = 2.9e-07
Identity = 27/64 (42.19%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E++   +  E    +I+E  ++A +C R+ GEERP MKEVA +LE LRV   +H+
Sbjct: 631 ENRLDEIIGGEVMNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHK 690

Query: 531 WVNE 534
           W ++
Sbjct: 691 WSDQ 694

BLAST of Cp4.1LG01g20800 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 305.1 bits (780), Expect = 1.2e-82
Identity = 189/483 (39.13%), Postives = 271/483 (56.11%), Query Frame = 0

Query: 11  LMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDP 70
           LM +   +  + +  +Q    C  RCGD+ I YPFG   GCY   + +F ITC     D 
Sbjct: 8   LMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DK 67

Query: 71  PRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTKNK 130
           P       NI+V N + SG+L+ L   +  CY +  + D       L+  +F   S  NK
Sbjct: 68  PNVL---SNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF---SPNNK 127

Query: 131 FTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLRYR 190
           FT++GC+ +A LS     Q+Y T C++LCD      +  C+G GCC+ ++   L   R  
Sbjct: 128 FTLVGCNAWALLS-TFGIQNYSTGCMSLCDT-PPPPNSKCNGVGCCRTEVSIPLDSHRIE 187

Query: 191 VR--SFDNHTDVLSFNPCGYAFVTEEDKFHFSAAY-IRDFPQ-RQVPVVLDWGISNTTCS 250
            +   F+N T V  FNPC YAF  E+  F+FS+   ++D     + PV+LDW I N TC 
Sbjct: 188 TQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSIGNQTCE 247

Query: 251 TANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDCK- 310
               ++  +CG NS   +    G  Y C CL GF+GNPYL  GCQDI+EC   R+++C  
Sbjct: 248 QVVGRN--ICGGNSTCFDS-TRGKGYNCKCLQGFDGNPYLSDGCQDINEC-TTRIHNCSD 307

Query: 311 -FECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSK------SFVQVIIGVSVGFTVLVIG 370
              C NT G++ C CP G   D       C  + K       +  V++G ++GF ++++ 
Sbjct: 308 TSTCENTLGSFHCQCPSG--SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLT 367

Query: 371 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDE 430
            +++    R  K  +L+++FFE+NGG ML + LS    S   V IFT+E + +AT+ Y+E
Sbjct: 368 ISYIQQKMRHRKNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNE 427

Query: 431 SAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGE 480
           S ++G+GG GTVYKGIL D S+VAIKK++L D+SQ  QFINEV+VLSQINHRNV   LG 
Sbjct: 428 SRILGQGGQGTVYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGC 473


HSP 2 Score: 57.0 bits (136), Expect = 5.7e-08
Identity = 30/64 (46.88%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E+++  +  E    +I+E  ++A +C RI GEERPSMKEVA ELE LRV   +H+
Sbjct: 630 ENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEERPSMKEVAAELEALRVKTTKHQ 689

Query: 531 WVNE 534
           W ++
Sbjct: 690 WSDQ 693

BLAST of Cp4.1LG01g20800 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 301.2 bits (770), Expect = 1.7e-81
Identity = 185/468 (39.53%), Postives = 257/468 (54.91%), Query Frame = 0

Query: 32  CPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGEL 91
           C  +CG++ I YPFG   GCY   + NF +TC           L  G IQVTNIS SG +
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVV-----EEKLLLFGIIQVTNISHSGHV 90

Query: 92  QILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTKNKFTVIGCDTYAFLSGQIEGQSY 151
            +L     +CY + N  +       L  S+F++SS  NKFT++GC+  + LS     Q+Y
Sbjct: 91  SVLFERFSECYEQKNETNGTALGYQLG-SSFSLSS-NNKFTLVGCNALSLLS-TFGKQNY 150

Query: 152 RTACVALCDNITTVRDGACSGNGCC---QLDIPSGLKGLRY---RVRSFDN------HTD 211
            T C++LC N     +G C+G GCC      +P      ++   R+R+  N      +T 
Sbjct: 151 STGCLSLC-NSQPEANGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVNNSLDLFNTS 210

Query: 212 VLSFNPCGYAFVTEEDKFHFSAAYIRDFPQ----RQVPVVLDWGISNTTCSTANKKSNCV 271
           V  FNPC YAF+ E+ KF+F ++  +D        + PV LDW I N TC  A   S  +
Sbjct: 211 VYQFNPCTYAFLVEDGKFNFDSS--KDLKNLRNVTRFPVALDWSIGNQTCEQAG--STRI 270

Query: 272 CGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLN--DCKFECVNTEG 331
           CG NS   N       Y C C +G++GNPY   GC+DIDEC  +  N  D K  C N +G
Sbjct: 271 CGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPK-TCRNRDG 330

Query: 332 NYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYRKWKLIK 391
            + C CP G+          CTR      ++ + + +G  VL++ +  +    ++ K  K
Sbjct: 331 GFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKYTK 390

Query: 392 LKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGYGTVYKG 451
           L+ +FFE+NGG ML + LS    S     IFT+E + +ATN YDES ++G+GG GTVYKG
Sbjct: 391 LRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVYKG 450

Query: 452 ILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVE 480
           ILPD ++VAIKK++L D  Q  QFI+EV+VLSQINHRNV   LG  +E
Sbjct: 451 ILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLE 480


HSP 2 Score: 56.2 bits (134), Expect = 9.8e-08
Identity = 27/64 (42.19%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 471 EDRLGEVVEKGMAREGEFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHE 530
           E+RL E+++  +  E    +I+E  ++A +C R+ GEERP MKEVA +LE LRV   +H+
Sbjct: 637 ENRLHEIIDDQVLNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHK 696

Query: 531 WVNE 534
           W ++
Sbjct: 697 WSDQ 700

BLAST of Cp4.1LG01g20800 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 295.8 bits (756), Expect = 7.4e-80
Identity = 182/487 (37.37%), Postives = 271/487 (55.65%), Query Frame = 0

Query: 10  RLMIVNILILS-SPVYASQALLGCPDRCGDLQIPYPFGTREGCYL--NKNFLITCNTTHF 69
           RL +V I  LS   +   Q L  CP++CG++ + YPFG   GC+   + +F ++C   + 
Sbjct: 5   RLFLVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNEN- 64

Query: 70  DPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNLSTFTVSSTK 129
                 L    ++V  IS S +L++L+  +  CY       A+ T    NL   T+S   
Sbjct: 65  ------LFYKGLEVVEISHSSQLRVLYPASYICYNSKGKF-AKGTYYWSNLGNLTLSG-N 124

Query: 130 NKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGLR 189
           N  T +GC++YAF+S     ++    C++ CD ++   +G C+G GCCQ  +P+G   L 
Sbjct: 125 NTITALGCNSYAFVSSNGTRRN-SVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLI 184

Query: 190 YRVRSFDNHTDV--LSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQ---VPVVLDWGISNT 249
            R   FDN T V  +S   C YAF+ E  KF ++A+    + Q +    PVVLDW I   
Sbjct: 185 VRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGE 244

Query: 250 TCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDE---R 309
           TC    +K    CG N +  N    G  Y C C  GF+GNPYL  GCQDI+EC       
Sbjct: 245 TCGQVGEKK---CGVNGICSNS-ASGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIH 304

Query: 310 LNDCKFE--CVNTEGNYTCNCPEGFKGDGRRGGEGC-TRSSKSFVQ---VIIGVSVGFTV 369
            ++C  +  C N  G++ CNC   ++ +       C  + +  +V+   +++G ++GF V
Sbjct: 305 KHNCSGDSTCENKLGHFRCNCRSRYELNTTT--NTCKPKGNPEYVEWTTIVLGTTIGFLV 364

Query: 370 LVIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATN 429
           +++  + +    +  K  +L+++FFE+NGG ML + LS    S   V IFT+E + +AT+
Sbjct: 365 ILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATD 424

Query: 430 KYDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVED 480
            YDE+ ++G+GG GTVYKGILPD S+VAIKK++L D SQ  QFINEV+VLSQINHRNV  
Sbjct: 425 GYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVK 475


HSP 2 Score: 58.2 bits (139), Expect = 2.6e-08
Identity = 34/93 (36.56%), Postives = 56/93 (60.22%), Query Frame = 0

Query: 442 KKSKLVDQSQTSQFINEVIVLSQINHRNVEDRLGEVVEKGMAREGEFGQIKEVGKVARKC 501
           +K+   ++ QTS+ I     +S       E+RL E+++  +  E    +I++  ++A +C
Sbjct: 608 QKALCFERPQTSKHI-----VSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVEC 667

Query: 502 LRISGEERPSMKEVAMELEGLRV-MVEHEWVNE 534
            R++GEERP MKEVA ELE LRV   +H+W +E
Sbjct: 668 TRLTGEERPGMKEVAAELEALRVTKTKHKWSDE 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMP12.0e-9842.47Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q391914.3e-8540.00Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN71.7e-8139.13Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q9LMN82.5e-8039.53Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN61.0e-7837.37Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_023512416.10.077.55wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022960687.10.075.20wall-associated receptor kinase 3-like [Cucurbita moschata][more]
XP_022989770.10.074.36wall-associated receptor kinase 2-like [Cucurbita maxima][more]
XP_022141581.16.53e-27055.66putative wall-associated receptor kinase-like 16 [Momordica charantia][more]
KAG6602141.15.83e-25697.39Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1H8430.075.20wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JNA50.074.36wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1CJM03.16e-27055.66putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A1S3B3B12.26e-23549.35wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 P... [more]
A0A5D3DKI32.77e-21246.51Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT1G21270.11.4e-9942.47wall-associated kinase 2 [more]
AT1G21250.13.1e-8640.00cell wall-associated kinase [more]
AT1G21230.11.2e-8239.13wall associated kinase 5 [more]
AT1G21240.11.7e-8139.53wall associated kinase 3 [more]
AT1G21210.17.4e-8037.37wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000742EGF-like domainSMARTSM00181egf_5coord: 293..333
e-value: 0.014
score: 24.6
coord: 242..289
e-value: 59.0
score: 7.2
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 290..324
score: 9.150207
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 290..333
e-value: 5.9E-7
score: 39.1
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 290..324
e-value: 1.1E-6
score: 28.7
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 32..87
e-value: 1.2E-9
score: 38.4
NoneNo IPR availableGENE3D2.90.20.10Plasmodium vivax P25 domaincoord: 234..339
e-value: 2.1E-12
score: 49.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 386..483
e-value: 2.0E-21
score: 78.0
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 26..470
coord: 471..541
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 26..470
coord: 471..541
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..32
score: 5.0
NoneNo IPR availableCDDcd00054EGF_CAcoord: 290..324
e-value: 5.09925E-7
score: 44.1646
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 289..325
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 418..480
e-value: 1.8E-5
score: 24.1
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 290..315
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 306..317
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 420..443
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 414..570
score: 10.209708
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 392..481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g20800.1Cp4.1LG01g20800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity