Cp4.1LG01g20760 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g20760
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCalcium-binding EF-hand family protein
LocationCp4.1LG01: 17637197 .. 17637670 (+)
RNA-Seq ExpressionCp4.1LG01g20760
SyntenyCp4.1LG01g20760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCCTATTATTACCATTAACGACTTGCTCCGCATCTTCAACAAGCTCGACATGAACCGCGATGGCCTCATTTGTCTACACGACCTCAACTGGCTCCTCGACAAGATCGGCGTCGAGCTAACCATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCCATGTCAACGCAAGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAATGATGATGGGTTCATTTCTTGTGAGGAGCTCCAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACATTGATTGTAAAAGTATGATCCGAGCTTATGATACCAATTTGGATGGGATGCTCGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

mRNA sequence

ATGTCTCCTATTATTACCATTAACGACTTGCTCCGCATCTTCAACAAGCTCGACATGAACCGCGATGGCCTCATTTGTCTACACGACCTCAACTGGCTCCTCGACAAGATCGGCGTCGAGCTAACCATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCCATGTCAACGCAAGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAATGATGATGGGTTCATTTCTTGTGAGGAGCTCCAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACATTGATTGTAAAAGTATGATCCGAGCTTATGATACCAATTTGGATGGGATGCTCGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

Coding sequence (CDS)

ATGTCTCCTATTATTACCATTAACGACTTGCTCCGCATCTTCAACAAGCTCGACATGAACCGCGATGGCCTCATTTGTCTACACGACCTCAACTGGCTCCTCGACAAGATCGGCGTCGAGCTAACCATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCCATGTCAACGCAAGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAATGATGATGGGTTCATTTCTTGTGAGGAGCTCCAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACATTGATTGTAAAAGTATGATCCGAGCTTATGATACCAATTTGGATGGGATGCTCGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

Protein sequence

MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLELWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Homology
BLAST of Cp4.1LG01g20760 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 4.3e-27
Identity = 70/156 (44.87%), Postives = 99/156 (63.46%), Query Frame = 0

Query: 5   ITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIG-VELTMEDLHSFLEKPSLDFNEFL-F 64
           IT NDL R+F  LD N+DGL+ L +L W+LDK+G  E T ++L   + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  FYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL--- 124
           +Y+++      + KG   +++   ++ + +  AF VFD+N DG+IS EEL+ VL RL   
Sbjct: 66  YYDAVL-----DSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFE 125

Query: 125 ---ELWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMM 153
              + W    DC  MIR +D NLDG +DFEEFKNM+
Sbjct: 126 EEAKAW----DCGRMIRVHDKNLDGFVDFEEFKNMI 152

BLAST of Cp4.1LG01g20760 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.6e-16
Identity = 61/163 (37.42%), Postives = 87/163 (53.37%), Query Frame = 0

Query: 2   SPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLE------KPSL 61
           SP +    L RIF+  D N DG I + +L+  L ++G+   + DL S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFNEFLFFYESISKQ-NKGECKGGVSH---VNAREEDIDIVYMAFKVFDMNDDGFISCEE 121
           +F++F   ++++      G C GG +     +A E + D+   AFKVFD N DGFIS  E
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLA-EAFKVFDENGDGFISARE 141

Query: 122 LQLVLSRLELWDVN--IDCKSMIRAYDTNLDGMLDFEEFKNMM 153
           LQ VL +L L +       + MI + D N DG +DF EFKNMM
Sbjct: 142 LQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Cp4.1LG01g20760 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.5e-15
Identity = 58/152 (38.16%), Postives = 80/152 (52.63%), Query Frame = 0

Query: 9   DLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSL------DFNEFLF 68
           +L RIF   D N DG I   +LN  L+ +G+ +  +DL   +EK  L      D  EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 69  FYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLELW 128
            Y++I ++              R+E+ D +  AF VFD N DGFI+ EEL+ VL+ L L 
Sbjct: 65  LYQTIMEE--------------RDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLK 124

Query: 129 DVNI--DCKSMIRAYDTNLDGMLDFEEFKNMM 153
                 DCK MI   D + DGM++F+EFK MM
Sbjct: 125 QGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

BLAST of Cp4.1LG01g20760 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-14
Identity = 56/162 (34.57%), Postives = 86/162 (53.09%), Query Frame = 0

Query: 2   SPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFL------EKPSL 61
           SP +    L R+F+  D N DG I + +L+  L ++G++    DL S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFNEFLFFYESISKQ---NKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEEL 121
            F++F   ++++ +     +G C  G     + E D++    AF VFD + DGFIS  EL
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSCCDG-----SPESDLE---EAFNVFDEDGDGFISAVEL 140

Query: 122 QLVLSRLELWDVN--IDCKSMIRAYDTNLDGMLDFEEFKNMM 153
           Q VL +L L +       + MI + D+N DG +DF EFKNMM
Sbjct: 141 QKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Cp4.1LG01g20760 vs. ExPASy Swiss-Prot
Match: Q2R1Z5 (Putative calmodulin-like protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CML6 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.1e-13
Identity = 50/151 (33.11%), Postives = 82/151 (54.30%), Query Frame = 0

Query: 7   INDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEFLFFYES 66
           I+D    F+  D N DG I   +L  +L ++G+  + EDL   +     D N  + F E 
Sbjct: 10  ISDFRDAFSLFDKNNDGCISREELATVLTRLGMAPSQEDLQDMIVAVDEDGNGTIEFDEF 69

Query: 67  ISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL--ELWDV 126
           ++   K        + N + +D + +  AF++FD +D+GFIS  EL +V++ L  E+ + 
Sbjct: 70  LAIMKK------KLYENDKGDDEEELRKAFRIFDKDDNGFISRNELSMVMASLGEEMTED 129

Query: 127 NIDCKSMIRAYDTNLDGMLDFEEFKNMMSLT 156
            ID   M++A D+N DG +D+EEFK +M  T
Sbjct: 130 EID--DMMKAADSNNDGQVDYEEFKRVMMST 152

BLAST of Cp4.1LG01g20760 vs. NCBI nr
Match: XP_023527806.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321 bits (822), Expect = 1.27e-110
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120

Query: 121 LWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           LWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 121 LWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157

BLAST of Cp4.1LG01g20760 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302 bits (773), Expect = 4.19e-103
Identity = 150/160 (93.75%), Postives = 153/160 (95.62%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPII+INDLLRIFNKLD NRDGLICLH+LNWLLD IGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVS VNAREED+DIVYMAFKVFDMNDDGFISCEELQLVLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120

Query: 121 LWDVNI---DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           LWDVN    DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 121 LWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

BLAST of Cp4.1LG01g20760 vs. NCBI nr
Match: XP_022959484.1 (probable calcium-binding protein CML44 [Cucurbita moschata])

HSP 1 Score: 299 bits (766), Expect = 4.90e-102
Identity = 148/160 (92.50%), Postives = 152/160 (95.00%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPII+INDLLRIFNKLD NRDGLICLH+LNWLLD IGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVS VNAREED+D+VYMAFKVFDMNDDGFISCEELQLVLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSDVNAREEDVDVVYMAFKVFDMNDDGFISCEELQLVLSRLE 120

Query: 121 LWDVNI---DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           LWDVN    DCKSMIRAYDTNLDGMLDFEEFKNMMSL SS
Sbjct: 121 LWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

BLAST of Cp4.1LG01g20760 vs. NCBI nr
Match: XP_022989807.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 297 bits (760), Expect = 4.03e-101
Identity = 147/160 (91.88%), Postives = 152/160 (95.00%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPII INDLLRIFNKLD NRDGLICLH+L+WLLD IGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVS VNARE+D+DIVYMAFKVFDMNDDGFISCEELQ VLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSDVNAREDDVDIVYMAFKVFDMNDDGFISCEELQHVLSRLE 120

Query: 121 LWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           +WDVN   IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 121 IWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

BLAST of Cp4.1LG01g20760 vs. NCBI nr
Match: KAG6578937.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238 bits (608), Expect = 5.92e-78
Identity = 117/160 (73.12%), Postives = 137/160 (85.62%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           M+  I+ NDLLRIFN+LD N+DGLIC+ +L WLLD IG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNA-REEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL 120
           LFFYESISKQNKGECKGG+ + +   EED++IVYMAFKVFD+N DGFISCEEL+ VLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVYEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTS 156
           ELWD N   +DC+SMIRAYDTNLDG LDFEEF NMM  T+
Sbjct: 121 ELWDANKNEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

BLAST of Cp4.1LG01g20760 vs. ExPASy TrEMBL
Match: A0A6J1H868 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111460440 PE=4 SV=1)

HSP 1 Score: 299 bits (766), Expect = 2.37e-102
Identity = 148/160 (92.50%), Postives = 152/160 (95.00%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPII+INDLLRIFNKLD NRDGLICLH+LNWLLD IGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVS VNAREED+D+VYMAFKVFDMNDDGFISCEELQLVLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSDVNAREEDVDVVYMAFKVFDMNDDGFISCEELQLVLSRLE 120

Query: 121 LWDVNI---DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           LWDVN    DCKSMIRAYDTNLDGMLDFEEFKNMMSL SS
Sbjct: 121 LWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

BLAST of Cp4.1LG01g20760 vs. ExPASy TrEMBL
Match: A0A6J1JGU4 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111486883 PE=4 SV=1)

HSP 1 Score: 297 bits (760), Expect = 1.95e-101
Identity = 147/160 (91.88%), Postives = 152/160 (95.00%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPII INDLLRIFNKLD NRDGLICLH+L+WLLD IGVELTMEDLHSFLEKPSLDFNEF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLE 120
           LFFYESISKQNKGECKGGVS VNARE+D+DIVYMAFKVFDMNDDGFISCEELQ VLSRLE
Sbjct: 61  LFFYESISKQNKGECKGGVSDVNAREDDVDIVYMAFKVFDMNDDGFISCEELQHVLSRLE 120

Query: 121 LWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
           +WDVN   IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 121 IWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

BLAST of Cp4.1LG01g20760 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 238 bits (607), Expect = 4.07e-78
Identity = 118/160 (73.75%), Postives = 135/160 (84.38%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           M+  I+ NDLLRIFN+LD N+DGLIC+ +L WLLD IG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGG-VSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL 120
           LFFYESISKQNKGECKGG V      EED++IVYMAFKVFD+N DGFISCEEL+ VLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTS 156
           ELWD N   +DC+SMIRAYDTNLDG LDFEEF NMM  T+
Sbjct: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

BLAST of Cp4.1LG01g20760 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 237 bits (604), Expect = 1.43e-77
Identity = 121/167 (72.46%), Postives = 137/167 (82.04%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           MSPI + NDLL+IFNKLD N DGL+CL +L+WLLD+IG++LTME+L S LEKPSLDFNEF
Sbjct: 1   MSPICS-NDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEF 60

Query: 61  LFFYESISKQNKGECKGGVSHVNAR-------EEDIDIVYMAFKVFDMNDDGFISCEELQ 120
           LFFYESISK+NKGEC GGV             EED+DIVYMAFKVFDMNDDGFISCEEL+
Sbjct: 61  LFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELR 120

Query: 121 LVLSRLELWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157
            VL RLELWD N    +C++MIRAYDTNLDG LDFEEFKNMM LT+S
Sbjct: 121 SVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

BLAST of Cp4.1LG01g20760 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 236 bits (601), Expect = 3.34e-77
Identity = 116/160 (72.50%), Postives = 134/160 (83.75%), Query Frame = 0

Query: 1   MSPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEF 60
           M+P I+ NDLLRIFN+LD N DGLIC+ +L WLLD IG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGG-VSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL 120
           L FYES+SKQNKGECKGG V      +ED++IVYMAFKVFD+N DGFISCEEL+ VLSRL
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTS 156
           ELWD N   +DC+SMIRAYDTNLDG LDFEEF NMM  T+
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

BLAST of Cp4.1LG01g20760 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.1e-28
Identity = 70/156 (44.87%), Postives = 99/156 (63.46%), Query Frame = 0

Query: 5   ITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIG-VELTMEDLHSFLEKPSLDFNEFL-F 64
           IT NDL R+F  LD N+DGL+ L +L W+LDK+G  E T ++L   + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  FYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRL--- 124
           +Y+++      + KG   +++   ++ + +  AF VFD+N DG+IS EEL+ VL RL   
Sbjct: 66  YYDAVL-----DSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFE 125

Query: 125 ---ELWDVNIDCKSMIRAYDTNLDGMLDFEEFKNMM 153
              + W    DC  MIR +D NLDG +DFEEFKNM+
Sbjct: 126 EEAKAW----DCGRMIRVHDKNLDGFVDFEEFKNMI 152

BLAST of Cp4.1LG01g20760 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 85.1 bits (209), Expect = 5.4e-17
Identity = 61/163 (37.42%), Postives = 87/163 (53.37%), Query Frame = 0

Query: 2   SPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLE------KPSL 61
           SP +    L RIF+  D N DG I + +L+  L ++G+   + DL S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFNEFLFFYESISKQ-NKGECKGGVSH---VNAREEDIDIVYMAFKVFDMNDDGFISCEE 121
           +F++F   ++++      G C GG +     +A E + D+   AFKVFD N DGFIS  E
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLA-EAFKVFDENGDGFISARE 141

Query: 122 LQLVLSRLELWDVN--IDCKSMIRAYDTNLDGMLDFEEFKNMM 153
           LQ VL +L L +       + MI + D N DG +DF EFKNMM
Sbjct: 142 LQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Cp4.1LG01g20760 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 82.0 bits (201), Expect = 4.6e-16
Identity = 58/152 (38.16%), Postives = 80/152 (52.63%), Query Frame = 0

Query: 9   DLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSL------DFNEFLF 68
           +L RIF   D N DG I   +LN  L+ +G+ +  +DL   +EK  L      D  EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 69  FYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLELW 128
            Y++I ++              R+E+ D +  AF VFD N DGFI+ EEL+ VL+ L L 
Sbjct: 65  LYQTIMEE--------------RDEEED-MREAFNVFDQNRDGFITVEELRSVLASLGLK 124

Query: 129 DVNI--DCKSMIRAYDTNLDGMLDFEEFKNMM 153
                 DCK MI   D + DGM++F+EFK MM
Sbjct: 125 QGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

BLAST of Cp4.1LG01g20760 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 80.5 bits (197), Expect = 1.3e-15
Identity = 56/162 (34.57%), Postives = 86/162 (53.09%), Query Frame = 0

Query: 2   SPIITINDLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFL------EKPSL 61
           SP +    L R+F+  D N DG I + +L+  L ++G++    DL S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFNEFLFFYESISKQ---NKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEEL 121
            F++F   ++++ +     +G C  G     + E D++    AF VFD + DGFIS  EL
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSCCDG-----SPESDLE---EAFNVFDEDGDGFISAVEL 140

Query: 122 QLVLSRLELWDVN--IDCKSMIRAYDTNLDGMLDFEEFKNMM 153
           Q VL +L L +       + MI + D+N DG +DF EFKNMM
Sbjct: 141 QKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Cp4.1LG01g20760 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 74.7 bits (182), Expect = 7.3e-14
Identity = 49/145 (33.79%), Postives = 76/145 (52.41%), Query Frame = 0

Query: 9   DLLRIFNKLDMNRDGLICLHDLNWLLDKIGVELTMEDLHSFLEKPSLDFNEFLFFYESIS 68
           +L ++F+K D N DG I + +L  +   +G   T E+L+  L++  +D + F+   E  +
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 69  KQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMNDDGFISCEELQLVLSRLELWDVNIDC 128
                 C+   S V  RE        AF ++D N +G IS  E+  VL+RL +     DC
Sbjct: 80  -----ICRSSSSAVEIRE--------AFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDC 139

Query: 129 KSMIRAYDTNLDGMLDFEEFKNMMS 154
             MI   DT+ DG ++FEEF+ MMS
Sbjct: 140 VRMIGHVDTDGDGNVNFEEFQKMMS 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPK54.3e-2744.87Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG97.6e-1637.42Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q9SRR76.5e-1538.16Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1[more]
Q9FI191.9e-1434.57Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
Q2R1Z56.1e-1333.11Putative calmodulin-like protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
XP_023527806.11.27e-110100.00probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
KAG6602129.14.19e-10393.75putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022959484.14.90e-10292.50probable calcium-binding protein CML44 [Cucurbita moschata][more]
XP_022989807.14.03e-10191.88probable calcium-binding protein CML44 [Cucurbita maxima][more]
KAG6578937.15.92e-7873.13putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
A0A6J1H8682.37e-10292.50probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JGU41.95e-10191.88probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1FLU54.07e-7873.75probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1CJ361.43e-7772.46probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1K1Y73.34e-7772.50probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893... [more]
Match NameE-valueIdentityDescription
AT1G21550.13.1e-2844.87Calcium-binding EF-hand family protein [more]
AT4G20780.15.4e-1737.42calmodulin like 42 [more]
AT3G07490.14.6e-1638.16ARF-GAP domain 11 [more]
AT5G44460.11.3e-1534.57calmodulin like 43 [more]
AT1G73630.17.3e-1433.79EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 91..119
e-value: 0.32
score: 20.1
coord: 9..37
e-value: 1.3
score: 16.9
coord: 127..155
e-value: 3.9E-4
score: 29.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 93..153
e-value: 1.2E-7
score: 32.1
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 13..29
e-value: 0.0089
score: 15.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 123..157
score: 10.524363
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 5..40
score: 10.273304
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 87..122
score: 12.114401
IPR002048EF-hand domainCDDcd00051EFhcoord: 95..153
e-value: 2.37513E-12
score: 56.7873
NoneNo IPR availableGENE3D1.10.238.10coord: 6..70
e-value: 6.0E-7
score: 31.5
NoneNo IPR availableGENE3D1.10.238.10coord: 74..157
e-value: 3.0E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 9..157
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 9..157
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 100..112
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 18..30
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 7..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g20760.1Cp4.1LG01g20760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding