Cp4.1LG01g19460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g19460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiontransmembrane protein 53-like
LocationCp4.1LG01: 16576778 .. 16581830 (-)
RNA-Seq ExpressionCp4.1LG01g19460
SyntenyCp4.1LG01g19460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCGTGCTCGCTTTTCTTCTCTGCCATTCGTTTGTTCGTTCGATTCGTGTTCTTAAACGTAATCAAATTTCAGAAAAAACAAGTAACAAAGCAGTGTTTTGCTTTCGAGATTTCTCTCTTTCCTTCTCTCCTTCGACGGTATGTTTTGTTTTTGTTTTGTTTTTGTTTTTGTTTTTGTTTCTCTCTATGGACCTGCATTTTTCATTCTTCGATTCGATTTCATGTTCTGTGATTGTAGTTTTTGTGTTCCTGTTCCTGTTTGTTTTCTTGTGATTTTCTCTGTTTTGTTCTGATTTCGGGCTTATCCGTCGACGAGCGATTTTCGCGTTTTTCCCCTTTCTCGATTGATATTCTGGTGTTTCCCTTTCGGGCGGATTGAAGTCCGATGATAAGATTTGTTCAATGTCGATTATTTCACCTGATTTTTATAGATTCTGTTGCAATGGATTGAAATCTGTTTGAGGTTGAATTATAGGGTAATTTGGAGGGACGTGGCCTCCGATACCGATTTTACGACCCGGTATTAGTTTATAGGGAGGAATGTTTTTTGTAATTTCAATTTGTTAGTAGGTCAGGACCAGCGCATTGTGGAAAATTAATGTTCTTTCTCCGATTTGACCGTCTGTACTACAGAAAGAGAAGCACCCTGTGATTTTCTACGGAATTATTTGACGAATTAGTATCAAATTTTCTAATGTGAAAACATTAAATTGATTTGTTTGAAGTACGAGGGGTGATTTTCTATTGAATTACTTTATTTTTTGAGACCCGTTTCCAAATTCTCATACAGAGTTTGAAAATCAACGGGGGGGTTTTTTCCTGACCAAGTCAGCGTGGGATTATGACTTGTCGTGCGAAACGTGCGTCGTTTATCTGCCGTTCTTGTAGGGCTTGAATAGATTCATCTTTTTTGAGTTTGAGGTTAGCGTAGTCGATCCGGATGGTTCTTGCACTGTTTATCAAATTAATTTGTTTACTTAGATTGGAAATTAATCGGTATGTTTGTTGTCTGGTCCACTTTTTCATTCTTCTCAACATTTGTTATGGACGGCAACGCATAAGTTACACATCCTTATCAATGGTCTCTTCTTTGTGGGGCTTCTGGTTTTTCTGGAGTTGTGTCGCTATCCTCACCGAGTTCTTTGGCTGGAGTCACAACACGTGGCGTCAACTAATTCTTTTTCCTTTTTCTCTTTTTGGGGATGTTCCCAATTCCTAGTTTAAAAATGTAATGCAATTTAATCGTAACACTTGGAGACGTTCCTTTTCCTGTTTCAGGAACGTGCGTGGGTGGGAACGGGAAGAGGGCTTGACCAAACCCACCATTTTCAAGGAAACTTCTTGGACGGTTTGGTTCGAGGATAAACATAAGATCCGGCTGTCAAAGTAGGTGACAGCCTGATTTTCTTGAACAGTTTCAAAGATCTATTCGAAGATCCTTTTTCTTGTGCGTATGACCAAACTTTGATACTTGGACCTCCCTTTTTATATGTGCATCTTGTTTACTCATTAACGCGTTCAGTTTTTTATTTCTTGGTACAGCAGGTGATCTCATAGAAAGGCCCCGCTTTCTGGGTCGTCTACATCTTAGATTGCTCGACCGTTGGTTTTTGCTTAATGTTCTTTCAGAGTTATCTGCGATTGGCATGAGATATTTTGATTTGTGTTTTAGATATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCATTGCTGTAACTGCTGTGGCCGTTGCTTCTATTTCCTCTGACTTTTCAGATAAGTTACCATCTCAGAGACCAACAGAGGCTGCTTCTTCTTCTTCTTTTTCAGATTTATCCCATAATTCAACTTTAGATTTCTTAAAGGAATCAAGGACGTCATTTGCTTTTGTTTCAAAGCTTGAAAACTTATCATTTGTCACCAAAATTCATGCACCTGTCCTTAAGTTTAGCTTTCCGATTCCAACTACCAGTTGCGACTATGTTAGAAACTTGCACGCGTCTTTGGTGTCTTCCACCGTTCTCTCTAATTTATATCAGTCAGCTGCATTGACCAAGGCATCAAAACAGGTTGCACCTTCACCTAAATTTTCTTCCCCACCTTCTGAGGTTATGTACAGATGGCATTTACCAGAGCCAACTTCTTTAGATATTAGCGGTAGTTCTGCTTGTTCCGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAGAAACATTTAAAGAGATACGCCGAATGGTATACTTCAAGGGGTTTTCATGCCATTACCTTTACTTTTCCAATGGCTGAGATACTCACGTATCAACTTGATGGGAAAGTTGAACAGCACATTGACTTGCTTGTGAATCATCTTGCTGATTGGTTGGAAGAGGAGCACGGAAAGAACCTGGTGTTTCACACTTTTAGCAATACTGGATGGTTAACGTACGTTATTTTTTCCCTCTTCATTTAACGTGCACACAGAGTGATAGCACCTTTGGCTTTTCTTGAACAAATTTCTTTATGTGATTGTTCCTTTGGATTTATCTCGATGCAGTTATGGAGCTATTCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAGGGCTGCATTGTAGATTCTGCCCCTGTTGCAGCTCCTGACCCTCAGGTAAATGGCGGCCTTGATGTGACAGTATTGTTCTTTGTCAATGAGATGTTACTATTGCATCATTTTGACCCCAGTTCCCTTTCTTTTCATTCAAAAATTTGTAGCTCGCCGTTACACGCACCACAATAAGTAACCTTGAAATATGAAGCTTTCTTTGATCATGGGATGACCATCAACCTATCCCAAACTCAGTCACCATCGCATCAACCTATCATAGAATTATATACAAAAAAACTAGAGTAGCCATTTTTACCTTGCATTTCCTCTATATATCAAGTTATAGATAAGGATAAATTTTTGTTTGTTCAACTCCTTATTTTCTCTATGAATTTAGAGTTAAGAATACCTTTTTGTTGGTCATTTAGATTTCTACCGCCTCTTTTATTTAGAATTGTAGGCCGTCTCTGTGTTTCTATTTGAGTTTCCTGTTGAAGAAATTATGAGAGGAAGTGTTTGTTAAATTTTGTTAATGCTTTTGTAGGTCTGGGCATCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATTGGCAACCTTGGACAGCACAGGGATGGAAGTACCAATCAGCAGTAAGGAAGATATCGTACCTAAACCTGCAGTTACTGAAGCAGCACTTCTATTAGTTTTGGAGAAGATTTTTGGTGTTGTTTTGAAACTACCGACCGTGAACAGGTAGTTCCCCTTTTAAAAGTGATCATTATAACCAATATATACTAAACAAATGCAAGAAAATGATCCACATTGATTGGAGAGGGGAACGAGGGCCAGTGAGGACGCTGGGCCCCGAAAGGGAGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAATGACATTTCTTATAAGGGTGCGGAAACCTCTCCATATTAGACCCGTTTTAAAACCTGGAGGGAAAAGCCTAAAAACAACAATATCGATAAGCGGTGGGCTTGGGCGGTTACAATAAGCATGATAGTTTCGTTTCTTTTTACAAATATGTTTAGATTTTTTGTACTTCATTAACCAACCAGATCAAGAAATCTAAAAGACAAAAGTTGTCCTGTTTTTCTACTAGGAGGCTATCTGACGTGTTGGACACGTTATCATCGTCACAACCAAGCTGCCCTCAACTGTATATCTACAGCTCAGCAGATCGCGTTATCCCAGCCGATTCTGTGGAATCATTCATCAAAGAGCAACGACAAGCAGGACGAGAGGTTAGAGCTTGCAATTTTGTGTCCACCCCTCATGTAGATCATTTTAGGAATGACCCAAAACTATACACTTCTCAGCTTAGTCAGTTTTTAGCAGACTGTGTGCTTACTTGTTGTAGAGAGTCCCGTTAGCTCAAATTTGAGTTGGTCGCTCACCATTTTACCCGATTCTTTTCTTCTTTTTATACAACATTGTTCGTTCTTGTATACATGTGAGAGCATGCAAAAAGCCAACAATTCATTAGTTTTCAATATAGATCACTGTTCAATTGTATTTTTGTGGTGTTGATCATTCTTGACTTATGCTGGTGTGTGCTAGAACAAGAACATGAGTGTGTATAAGATGAACAAGATAGGACGAAGTTAAGTGTAAAACTTTACATAACAACGAAGTAGAGTTAATTTGGAAGCGAACACCGAACCTATATATAACATATCAACTTATATTGACACTAGTTGAATTTGTACAAATTGGATCTTATCTATCTATCTAGTCTTCCTTATCTCCAATAATTAAATTAAATATGAAGCACAAGAAAAACAAAAGAAAAGAAAACACTTCTACTTAACACATTTCCCCCAAGTACCAAACTTATAACTAGCAGTTAATCTTCCACTCCTGTTGAAGAAATCTGAATGTTTTTTGGAAATTCTGAAACCCCATGAACCAGAAATCGAAATTATCCACTGTTACAATCTCTAAGTACTTCTGTGAAGGCTTCATCACATTCTTACTTTGATTCACTCTCTCTATTCTTCCCACTGGAATCACCACCTGAATTTGTATCACAAAGAGTTCAGAAAACACGATAGAGGCTGAGCGAAATCTTAACGAGGGACAAAAAACGTTCGAATTCGGCAAAAGTAGAGAAAAATCTTTGGAATCATAACATTCTTTAGCTTAGTTTTGTGTTTAAGAGAGAGAGAGAGGTGACCTTGTAATGAAATTTTAGGAGCTCTCCAGTTGGGGAAGAGAGTTTGAGGGATTTGTCACTGCAAAAGGCAAGTTTGTGCGTTGATATGAAGAGAAGACCTGAAAGAGGTCCTGCTGTGGTTGACAAATGGCATTGACAAACCTTCAATAGCTTCTCTCCTTCTTTTAAACTAAATAGCTGCTTTCCTTTCCTCAATCCACCAACTTGAAGAATCTTTGCTCCTAAACTCAACTTTCCCTTCACTGTTTCTCTGATCTTTTGTCCTAATC

mRNA sequence

ACCGTGCTCGCTTTTCTTCTCTGCCATTCGTTTGTTCGTTCGATTCGTGTTCTTAAACGTAATCAAATTTCAGAAAAAACAAGTAACAAAGCAGTGTTTTGCTTTCGAGATTTCTCTCTTTCCTTCTCTCCTTCGACGGAACGTGCGTGGGTGGGAACGGGAAGAGGGCTTGACCAAACCCACCATTTTCAAGGAAACTTCTTGGACGGTTTGGTTCGAGGATAAACATAAGATCCGGCTGTCAAAGTAGGTGACAGCCTGATTTTCTTGAACAGTTTCAAAGATCTATTCGAAGATCCTTTTTCTTTTTTTTATTTCTTGGTACAGCAGGTGATCTCATAGAAAGGCCCCGCTTTCTGGGTCGTCTACATCTTAGATTGCTCGACCGTTGGTTTTTGCTTAATGTTCTTTCAGAGTTATCTGCGATTGGCATGAGATATTTTGATTTGTGTTTTAGATATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCATTGCTGTAACTGCTGTGGCCGTTGCTTCTATTTCCTCTGACTTTTCAGATAAGTTACCATCTCAGAGACCAACAGAGGCTGCTTCTTCTTCTTCTTTTTCAGATTTATCCCATAATTCAACTTTAGATTTCTTAAAGGAATCAAGGACGTCATTTGCTTTTGTTTCAAAGCTTGAAAACTTATCATTTGTCACCAAAATTCATGCACCTGTCCTTAAGTTTAGCTTTCCGATTCCAACTACCAGTTGCGACTATGTTAGAAACTTGCACGCGTCTTTGGTGTCTTCCACCGTTCTCTCTAATTTATATCAGTCAGCTGCATTGACCAAGGCATCAAAACAGGTTGCACCTTCACCTAAATTTTCTTCCCCACCTTCTGAGGTTATGTACAGATGGCATTTACCAGAGCCAACTTCTTTAGATATTAGCGGTAGTTCTGCTTGTTCCGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAGAAACATTTAAAGAGATACGCCGAATGGTATACTTCAAGGGGTTTTCATGCCATTACCTTTACTTTTCCAATGGCTGAGATACTCACGTATCAACTTGATGGGAAAGTTGAACAGCACATTGACTTGCTTGTGAATCATCTTGCTGATTGGTTGGAAGAGGAGCACGGAAAGAACCTGGTGTTTCACACTTTTAGCAATACTGGATGGTTAACTTATGGAGCTATTCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAGGGCTGCATTGTAGATTCTGCCCCTGTTGCAGCTCCTGACCCTCAGGTCTGGGCATCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATTGGCAACCTTGGACAGCACAGGGATGGAAGTACCAATCAGCAGTAAGGAAGATATCGTACCTAAACCTGCAGTTACTGAAGCAGCACTTCTATTAGTTTTGGAGAAGATTTTTGGTGTTGTTTTGAAACTACCGACCGTGAACAGGAGGCTATCTGACGTGTTGGACACGTTATCATCGTCACAACCAAGCTGCCCTCAACTGTATATCTACAGCTCAGCAGATCGCGTTATCCCAGCCGATTCTGTGGAATCATTCATCAAAGAGCAACGACAAGCAGGACGAGAGGTTAGAGCTTGCAATTTTGTGTCCACCCCTCATGTAGATCATTTTAGGAATGACCCAAAACTATACACTTCTCAGCTTAGTCAGTTTTTAGCAGACTGTGTGCTTACTTGTTGTAGAGAGTCCCGTTAGCTCAAATTTGAGTTGGTCGCTCACCATTTTACCCGATTCTTTTCTTCTTTTTATACAACATTGTTCGTTCTTGTATACATGTGAGAGCATGCAAAAAGCCAACAATTCATTAGTTTTCAATATAGATCACTGTTCAATTGTATTTTTGTGGTGTTGATCATTCTTGACTTATGCTGGTGTGTGCTAGAACAAGAACATGAGTGTGTATAAGATGAACAAGATAGGACGAAGTTAAGTGTAAAACTTTACATAACAACGAAGTAGAGTTAATTTGGAAGCGAACACCGAACCTATATATAACATATCAACTTATATTGACACTAGTTGAATTTGTACAAATTGGATCTTATCTATCTATCTAGTCTTCCTTATCTCCAATAATTAAATTAAATATGAAGCACAAGAAAAACAAAAGAAAAGAAAACACTTCTACTTAACACATTTCCCCCAAGTACCAAACTTATAACTAGCAGTTAATCTTCCACTCCTGTTGAAGAAATCTGAATGTTTTTTGGAAATTCTGAAACCCCATGAACCAGAAATCGAAATTATCCACTGTTACAATCTCTAAGTACTTCTGTGAAGGCTTCATCACATTCTTACTTTGATTCACTCTCTCTATTCTTCCCACTGGAATCACCACCTGAATTTGTATCACAAAGAGTTCAGAAAACACGATAGAGGCTGAGCGAAATCTTAACGAGGGACAAAAAACGTTCGAATTCGGCAAAAGTAGAGAAAAATCTTTGGAATCATAACATTCTTTAGCTTAGTTTTGTGTTTAAGAGAGAGAGAGAGGTGACCTTGTAATGAAATTTTAGGAGCTCTCCAGTTGGGGAAGAGAGTTTGAGGGATTTGTCACTGCAAAAGGCAAGTTTGTGCGTTGATATGAAGAGAAGACCTGAAAGAGGTCCTGCTGTGGTTGACAAATGGCATTGACAAACCTTCAATAGCTTCTCTCCTTCTTTTAAACTAAATAGCTGCTTTCCTTTCCTCAATCCACCAACTTGAAGAATCTTTGCTCCTAAACTCAACTTTCCCTTCACTGTTTCTCTGATCTTTTGTCCTAATC

Coding sequence (CDS)

ATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCATTGCTGTAACTGCTGTGGCCGTTGCTTCTATTTCCTCTGACTTTTCAGATAAGTTACCATCTCAGAGACCAACAGAGGCTGCTTCTTCTTCTTCTTTTTCAGATTTATCCCATAATTCAACTTTAGATTTCTTAAAGGAATCAAGGACGTCATTTGCTTTTGTTTCAAAGCTTGAAAACTTATCATTTGTCACCAAAATTCATGCACCTGTCCTTAAGTTTAGCTTTCCGATTCCAACTACCAGTTGCGACTATGTTAGAAACTTGCACGCGTCTTTGGTGTCTTCCACCGTTCTCTCTAATTTATATCAGTCAGCTGCATTGACCAAGGCATCAAAACAGGTTGCACCTTCACCTAAATTTTCTTCCCCACCTTCTGAGGTTATGTACAGATGGCATTTACCAGAGCCAACTTCTTTAGATATTAGCGGTAGTTCTGCTTGTTCCGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAGAAACATTTAAAGAGATACGCCGAATGGTATACTTCAAGGGGTTTTCATGCCATTACCTTTACTTTTCCAATGGCTGAGATACTCACGTATCAACTTGATGGGAAAGTTGAACAGCACATTGACTTGCTTGTGAATCATCTTGCTGATTGGTTGGAAGAGGAGCACGGAAAGAACCTGGTGTTTCACACTTTTAGCAATACTGGATGGTTAACTTATGGAGCTATTCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAGGGCTGCATTGTAGATTCTGCCCCTGTTGCAGCTCCTGACCCTCAGGTCTGGGCATCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATTGGCAACCTTGGACAGCACAGGGATGGAAGTACCAATCAGCAGTAAGGAAGATATCGTACCTAAACCTGCAGTTACTGAAGCAGCACTTCTATTAGTTTTGGAGAAGATTTTTGGTGTTGTTTTGAAACTACCGACCGTGAACAGGAGGCTATCTGACGTGTTGGACACGTTATCATCGTCACAACCAAGCTGCCCTCAACTGTATATCTACAGCTCAGCAGATCGCGTTATCCCAGCCGATTCTGTGGAATCATTCATCAAAGAGCAACGACAAGCAGGACGAGAGGTTAGAGCTTGCAATTTTGTGTCCACCCCTCATGTAGATCATTTTAGGAATGACCCAAAACTATACACTTCTCAGCTTAGTCAGTTTTTAGCAGACTGTGTGCTTACTTGTTGTAGAGAGTCCCGTTAG

Protein sequence

MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKESRTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDPKLYTSQLSQFLADCVLTCCRESR
Homology
BLAST of Cp4.1LG01g19460 vs. ExPASy Swiss-Prot
Match: Q6DHN0 (Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 9.4e-19
Identity = 77/279 (27.60%), Postives = 126/279 (45.16%), Query Frame = 0

Query: 168 VVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPM-----AEILTY-QLDGKVEQHIDL 227
           VV+LLGW G++ KHL +Y+  Y  +G   + +T P+     +E L Y +L     + ++L
Sbjct: 29  VVILLGWAGSRDKHLAKYSSIYNEQGCTTLRYTAPLKTVFISESLGYKELRSTAHKLLEL 88

Query: 228 LVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAA 287
           L ++      E     + FH FSN G++ Y  ++E    H       + G +VDSAP + 
Sbjct: 89  LYDY------EVENNPIFFHVFSNGGFMLYRYMVELLHSHKQFSTLCVVGTVVDSAPGS- 148

Query: 288 PDPQVWASGFSAAFLKKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEK 347
                           +N V    L  L +T           + PK  V     LL L  
Sbjct: 149 ----------------QNVVG--ALRALKTT-----------LGPKVNVLLQYFLLALFA 208

Query: 348 IFGVVLKL---PTVNRRLSDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQA 407
           +   +L++   P       +  D +       PQ+Y+YS ADRVI    VE  +K  ++ 
Sbjct: 209 VAVFLLRIVLYPLTKYFHRNHYDAMMEHPAPWPQMYLYSRADRVIRYRDVEKMVKGLQEK 268

Query: 408 GREVRACNFVSTPHVDHFRNDPKLYTSQLSQFLADCVLT 438
           G  V + +F++  HV  FR+ P+ Y+++   FL+ C+ T
Sbjct: 269 GLMVESFDFITPAHVSLFRDCPEDYSNRCRTFLSHCMTT 271

BLAST of Cp4.1LG01g19460 vs. ExPASy Swiss-Prot
Match: Q9D0Z3 (Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-18
Identity = 84/276 (30.43%), Postives = 118/276 (42.75%), Query Frame = 0

Query: 164 KSRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI--DL 223
           K + VV+LLGW G + K+L +Y+  Y  RG   I +T P   +   +  G     +    
Sbjct: 31  KQQPVVILLGWGGCRDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSESLGIPSLRVIAQK 90

Query: 224 LVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAA 283
           L+  L D+  E   + L+FH FSN G + Y  +LE  Q H       + G I DS P   
Sbjct: 91  LLELLFDY--EIEREPLLFHVFSNAGVMLYRYVLELLQTHQRFRHLHVVGTIFDSGP--- 150

Query: 284 PDPQVWASGFSAAFLKKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALL--LVL 343
                   G S         A + LAT+              +  +PAV    LL    L
Sbjct: 151 --------GDSNLI-----GALRALATI--------------LERRPAVLRLLLLAAFAL 210

Query: 344 EKIFGVVLKLPTVNRRLSDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAG 403
             I    L  P      +   D L  S    P+LY+YS AD+V+ A  VE  ++ +    
Sbjct: 211 VVILFHFLLAPFTALFHTHFYDRLQDSGSCWPELYLYSRADKVVSARDVERMVEARLAHQ 270

Query: 404 REVRACNFVSTPHVDHFRNDPKLYTSQLSQFLADCV 436
             VR  +FVS+ HV H R+ P  YTS    F+ +CV
Sbjct: 271 VMVRGVDFVSSAHVSHLRDYPTYYTSLCVDFMHNCV 274

BLAST of Cp4.1LG01g19460 vs. ExPASy Swiss-Prot
Match: Q6DJC8 (Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.9e-17
Identity = 79/300 (26.33%), Postives = 125/300 (41.67%), Query Frame = 0

Query: 139 EVMYRWHLPEPTSLDISGSSACSVAKSR-TVVVLLGWLGAKQKHLKRYAEWYTSRGFHAI 198
           E+ Y    PEP+         C     R  VV+LLGW G K ++L +Y+  Y ++G   I
Sbjct: 5   ELDYTIEFPEPS------LQGCPWDPEREPVVILLGWGGCKDQYLAKYSAIYHNQGCTVI 64

Query: 199 TFTFPMAEILTYQLDG--KVEQHIDLLVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAIL 258
            +T     +   +  G   + +    L+  L D+  E     ++FH FSN G++ Y  I+
Sbjct: 65  KYTAAWNAVFISESLGFSSLREDAKKLLELLFDY--EIEKSPILFHVFSNGGFMLYRYIV 124

Query: 259 EKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGLATLDSTGME 318
           E    H       + G I DSAP                    N      +  LD+    
Sbjct: 125 ELLHSHCRLNKLHVVGTIFDSAP-------------------GNRNVIGSVRALDT---- 184

Query: 319 VPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVLDTLSSSQPSCPQLYI 378
           +  +S  + +   A+   A+++++ +I    +  P       +  D +       PQLY+
Sbjct: 185 ILRTSTNNAIRFLALAAFAIMVIILRI----VLYPVTKFLHENHYDAMKKDSSRWPQLYL 244

Query: 379 YSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDPKLYTSQLSQFLADCV 436
           YS AD +I    VES I  +R+      A +F  + HV HFR  P  Y+   + FL DCV
Sbjct: 245 YSRADPIISYIDVESMIAARRRCCLPTEALDFGKSEHVSHFRRFPHRYSEMCTSFLRDCV 269

BLAST of Cp4.1LG01g19460 vs. ExPASy Swiss-Prot
Match: Q6P2H8 (Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 8.8e-17
Identity = 80/270 (29.63%), Postives = 118/270 (43.70%), Query Frame = 0

Query: 168 VVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI--DLLVNH 227
           VV+LLGW G K K+L +Y+  Y  RG   I +T P   +   +  G     +    L+  
Sbjct: 37  VVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSESLGIPSLRVLAQKLLEL 96

Query: 228 LADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQ 287
           L D+  E   + L+FH FSN G + Y  +LE  Q      + R+ G I DSAP       
Sbjct: 97  LFDY--EIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRL-RVVGTIFDSAP--GDSNL 156

Query: 288 VWASGFSAAFLKKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGV 347
           V A    AA L++ +   + L                       +   AL++VL      
Sbjct: 157 VGALRALAAILERRAAMLRLL----------------------LLVAFALVVVLFH---- 216

Query: 348 VLKLPTVNRRLSDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRAC 407
           VL  P      +   D L  +    P+LY+YS AD V+ A  +E  ++ +       R+ 
Sbjct: 217 VLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSV 275

Query: 408 NFVSTPHVDHFRNDPKLYTSQLSQFLADCV 436
           +FVS+ HV H R+ P  YTS    F+ +CV
Sbjct: 277 DFVSSAHVSHLRDYPTYYTSLCVDFMRNCV 275

BLAST of Cp4.1LG01g19460 vs. ExPASy Swiss-Prot
Match: Q2TBP5 (Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.1e-16
Identity = 77/272 (28.31%), Postives = 115/272 (42.28%), Query Frame = 0

Query: 168 VVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI--DLLVNH 227
           +V+LLGW G   K+L +Y+  Y  RG   I +T P   +   +  G     +    L+  
Sbjct: 37  LVILLGWGGCSDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSETLGIPSLRVLAQKLLEL 96

Query: 228 LADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQ 287
           L D+  E   + L+FH FSN G + Y  +LE  Q H      R+ G I DS P    D  
Sbjct: 97  LFDY--EVEKEPLLFHVFSNAGVMLYRYVLELLQTHQRFCHLRVVGTIFDSGP---GDSN 156

Query: 288 VWASGFSAAFLKKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGV 347
           +               A + LA +              +  +PA     LL+    +  +
Sbjct: 157 LLG-------------ALRALAVV--------------LEHRPAALRLLLLVAFTLVAFL 216

Query: 348 --VLKLPTVNRRLSDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVR 407
             VL  P      +   D L  +    P+LY+YS AD V+ A  VE  ++ +      VR
Sbjct: 217 FHVLLAPLTALFHTHFYDRLLDAASRWPELYLYSRADEVVLARDVERMVEARLAHQVLVR 276

Query: 408 ACNFVSTPHVDHFRNDPKLYTSQLSQFLADCV 436
           + +FVS+ HV H R+ P  YT+    F+  CV
Sbjct: 277 SVDFVSSAHVSHLRDYPTYYTTLCINFMHSCV 276

BLAST of Cp4.1LG01g19460 vs. NCBI nr
Match: XP_023552493.1 (transmembrane protein 53-like [Cucurbita pepo subsp. pepo] >XP_023552501.1 transmembrane protein 53-like [Cucurbita pepo subsp. pepo] >XP_023552509.1 transmembrane protein 53-like [Cucurbita pepo subsp. pepo] >XP_023552520.1 transmembrane protein 53-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864 bits (2233), Expect = 0.0
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLTCCRESR
Sbjct: 421 KLYTSQLSQFLADCVLTCCRESR 443

BLAST of Cp4.1LG01g19460 vs. NCBI nr
Match: KAG6601980.1 (Transmembrane protein 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 857 bits (2214), Expect = 3.26e-313
Identity = 439/443 (99.10%), Postives = 439/443 (99.10%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI LLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHISLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGL TLDSTGMEVPI SKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLTTLDSTGMEVPIGSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLTCCRESR
Sbjct: 421 KLYTSQLSQFLADCVLTCCRESR 443

BLAST of Cp4.1LG01g19460 vs. NCBI nr
Match: XP_022958510.1 (transmembrane protein 53-like [Cucurbita moschata] >XP_022958515.1 transmembrane protein 53-like [Cucurbita moschata] >XP_022958524.1 transmembrane protein 53-like [Cucurbita moschata])

HSP 1 Score: 855 bits (2210), Expect = 1.33e-312
Identity = 438/443 (98.87%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI LLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHISLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGL TLDSTGMEVPI SKEDIVPKPAVTE ALLLVLEKIFGVVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLTTLDSTGMEVPIGSKEDIVPKPAVTETALLLVLEKIFGVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLTCCRESR
Sbjct: 421 KLYTSQLSQFLADCVLTCCRESR 443

BLAST of Cp4.1LG01g19460 vs. NCBI nr
Match: XP_022990028.1 (transmembrane protein 53-like [Cucurbita maxima] >XP_022990029.1 transmembrane protein 53-like [Cucurbita maxima] >XP_022990030.1 transmembrane protein 53-like [Cucurbita maxima] >XP_022990031.1 transmembrane protein 53-like [Cucurbita maxima])

HSP 1 Score: 846 bits (2186), Expect = 6.03e-309
Identity = 433/443 (97.74%), Postives = 436/443 (98.42%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDK+PSQRPTEA SSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKIPSQRPTEATSSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQ+APSPKFSSPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQIAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPM EILTYQLDGKVEQHI LLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMTEILTYQLDGKVEQHIGLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGL TLDSTGMEVP+SSKEDIVPKPAVTEAALLLVLEKIF VVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLTTLDSTGMEVPMSSKEDIVPKPAVTEAALLLVLEKIFDVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLT CRESR
Sbjct: 421 KLYTSQLSQFLADCVLTSCRESR 443

BLAST of Cp4.1LG01g19460 vs. NCBI nr
Match: XP_008465096.1 (PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_008465105.1 PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_016903289.1 PREDICTED: transmembrane protein 53 [Cucumis melo])

HSP 1 Score: 765 bits (1976), Expect = 6.44e-277
Identity = 397/446 (89.01%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPF+A+TAVA+AS SSDFSDKLPSQ+PTE +SSSS S  S +STLDFLKES
Sbjct: 1   MGSLSGILQRPFVALTAVAIASFSSDFSDKLPSQKPTEDSSSSSSS--SSDSTLDFLKES 60

Query: 61  RTSFAF---VSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQS 120
            TSF     VSKL NLSFVTKIHAP+ KFSFPIPT+SC+YV N   SLVSS+VLSNLYQS
Sbjct: 61  NTSFVSHTSVSKLANLSFVTKIHAPIPKFSFPIPTSSCNYVGNFPTSLVSSSVLSNLYQS 120

Query: 121 AALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGA 180
           AALTK SKQVAPSPK SSPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLGA
Sbjct: 121 AALTKGSKQVAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLGA 180

Query: 181 KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKN 240
           KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQ+ GKVEQHIDLLVNHLADWLEEEHGKN
Sbjct: 181 KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQVGGKVEQHIDLLVNHLADWLEEEHGKN 240

Query: 241 LVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK 300
           LVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK
Sbjct: 241 LVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK 300

Query: 301 KNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLS 360
           KNSVATKGL T D+TGMEV IS KE+I PKPAVTEAALLL+LEKIFGVVL LPTVNRRLS
Sbjct: 301 KNSVATKGLTTWDNTGMEVSISDKENIEPKPAVTEAALLLLLEKIFGVVLNLPTVNRRLS 360

Query: 361 DVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFR 420
           DVLDTLSSSQPSCPQLYIYSSADRVIPA SVESFI+EQR+AGREVRACNFVSTPHVDHFR
Sbjct: 361 DVLDTLSSSQPSCPQLYIYSSADRVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHFR 420

Query: 421 NDPKLYTSQLSQFLADCVLT-CCRES 442
           NDPKLYT+QLSQFLADCVLT CCRES
Sbjct: 421 NDPKLYTTQLSQFLADCVLTACCRES 444

BLAST of Cp4.1LG01g19460 vs. ExPASy TrEMBL
Match: A0A6J1H3N4 (transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111459714 PE=3 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 6.42e-313
Identity = 438/443 (98.87%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHI LLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHISLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGL TLDSTGMEVPI SKEDIVPKPAVTE ALLLVLEKIFGVVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLTTLDSTGMEVPIGSKEDIVPKPAVTETALLLVLEKIFGVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLTCCRESR
Sbjct: 421 KLYTSQLSQFLADCVLTCCRESR 443

BLAST of Cp4.1LG01g19460 vs. ExPASy TrEMBL
Match: A0A6J1JP03 (transmembrane protein 53-like OS=Cucurbita maxima OX=3661 GN=LOC111487044 PE=3 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 2.92e-309
Identity = 433/443 (97.74%), Postives = 436/443 (98.42%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPFIAVTAVAVASISSDFSDK+PSQRPTEA SSSSFSDLSHNSTLDFLKES
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKIPSQRPTEATSSSSFSDLSHNSTLDFLKES 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
           RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL
Sbjct: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
           TKASKQ+APSPKFSSPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLGAKQK
Sbjct: 121 TKASKQIAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLGAKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLKRYAEWYTSRGFHAITFTFPM EILTYQLDGKVEQHI LLVNHLADWLEEEHGKNLVF
Sbjct: 181 HLKRYAEWYTSRGFHAITFTFPMTEILTYQLDGKVEQHIGLLVNHLADWLEEEHGKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS
Sbjct: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKGL TLDSTGMEVP+SSKEDIVPKPAVTEAALLLVLEKIF VVLKLPTVNRRLSDVL
Sbjct: 301 VATKGLTTLDSTGMEVPMSSKEDIVPKPAVTEAALLLVLEKIFDVVLKLPTVNRRLSDVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP
Sbjct: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420

Query: 421 KLYTSQLSQFLADCVLTCCRESR 443
           KLYTSQLSQFLADCVLT CRESR
Sbjct: 421 KLYTSQLSQFLADCVLTSCRESR 443

BLAST of Cp4.1LG01g19460 vs. ExPASy TrEMBL
Match: A0A1S3CN08 (transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103502788 PE=3 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 3.12e-277
Identity = 397/446 (89.01%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGILQRPF+A+TAVA+AS SSDFSDKLPSQ+PTE +SSSS S  S +STLDFLKES
Sbjct: 1   MGSLSGILQRPFVALTAVAIASFSSDFSDKLPSQKPTEDSSSSSSS--SSDSTLDFLKES 60

Query: 61  RTSFAF---VSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQS 120
            TSF     VSKL NLSFVTKIHAP+ KFSFPIPT+SC+YV N   SLVSS+VLSNLYQS
Sbjct: 61  NTSFVSHTSVSKLANLSFVTKIHAPIPKFSFPIPTSSCNYVGNFPTSLVSSSVLSNLYQS 120

Query: 121 AALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGA 180
           AALTK SKQVAPSPK SSPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLGA
Sbjct: 121 AALTKGSKQVAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLGA 180

Query: 181 KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKN 240
           KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQ+ GKVEQHIDLLVNHLADWLEEEHGKN
Sbjct: 181 KQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQVGGKVEQHIDLLVNHLADWLEEEHGKN 240

Query: 241 LVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK 300
           LVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK
Sbjct: 241 LVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLK 300

Query: 301 KNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLS 360
           KNSVATKGL T D+TGMEV IS KE+I PKPAVTEAALLL+LEKIFGVVL LPTVNRRLS
Sbjct: 301 KNSVATKGLTTWDNTGMEVSISDKENIEPKPAVTEAALLLLLEKIFGVVLNLPTVNRRLS 360

Query: 361 DVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFR 420
           DVLDTLSSSQPSCPQLYIYSSADRVIPA SVESFI+EQR+AGREVRACNFVSTPHVDHFR
Sbjct: 361 DVLDTLSSSQPSCPQLYIYSSADRVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHFR 420

Query: 421 NDPKLYTSQLSQFLADCVLT-CCRES 442
           NDPKLYT+QLSQFLADCVLT CCRES
Sbjct: 421 NDPKLYTTQLSQFLADCVLTACCRES 444

BLAST of Cp4.1LG01g19460 vs. ExPASy TrEMBL
Match: A0A0A0KMY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G352630 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 1.47e-273
Identity = 396/447 (88.59%), Postives = 414/447 (92.62%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHN-STLDFLKE 60
           MGSLSGILQRPF+A+TAVAVAS SSDFSDKLPSQ+PTEA+SSSS S  S + STLDFLKE
Sbjct: 1   MGSLSGILQRPFVALTAVAVASFSSDFSDKLPSQKPTEASSSSSSSSSSSSDSTLDFLKE 60

Query: 61  SRTSFAF---VSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQ 120
           S TSF     VSKL NLSFVTKIHAP+ KFSFP  ++SC+YV N   SLVSS+VLSNLYQ
Sbjct: 61  SNTSFVSHTSVSKLANLSFVTKIHAPIPKFSFP--SSSCNYVGNFPTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTK SKQ APSPK SSPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKGSKQAAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQL GKVEQHIDLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRL 360
           KKNSVATKGL T D+TGMEV IS KE+I PKPAVTEAALLLVLEKIFGVVL LPTVNRRL
Sbjct: 301 KKNSVATKGLTTWDNTGMEVSISDKENIKPKPAVTEAALLLVLEKIFGVVLNLPTVNRRL 360

Query: 361 SDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHF 420
           SDVLDTLSSSQPSCPQLYIYSSAD+VIPA SVESFI+EQR+AGREVRACNFVSTPHVDHF
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADQVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYTSQLSQFLADCVLT-CCRES 442
           RNDPKLYT+QLSQFLADCVLT CCRES
Sbjct: 421 RNDPKLYTTQLSQFLADCVLTSCCRES 445

BLAST of Cp4.1LG01g19460 vs. ExPASy TrEMBL
Match: A0A6J1CIU0 (transmembrane protein 53 OS=Momordica charantia OX=3673 GN=LOC111011995 PE=3 SV=1)

HSP 1 Score: 741 bits (1914), Expect = 9.67e-268
Identity = 389/447 (87.02%), Postives = 408/447 (91.28%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFS-DLSHNSTLDFLKE 60
           MGSLSGILQRPF AVTAVAVAS SSDFSDKLPSQR  +AA+SSS S DLS+NST DFLKE
Sbjct: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60

Query: 61  SRTSFAF---VSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQ 120
           S+T FA    VSKL NLSFVTKIHAPV   +FPIPT+S +YVRN H SLVSS VLSNLYQ
Sbjct: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120

Query: 121 SAALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQ A S K  SPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+ GKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRL 360
           KKNSVATKG   LD+T MEV I +K+D   KPAVTEAALL+VLEK FGVVL LPTVNRRL
Sbjct: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360

Query: 361 SDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHF 420
           SDVLD LSSSQPSCPQLYIYSSADRVIPA+SVESFI+EQR+AGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420

Query: 421 RNDPKLYTSQLSQFLADCVLT-CCRES 442
           RNDPKLY+SQLSQFLADCVLT CCRES
Sbjct: 421 RNDPKLYSSQLSQFLADCVLTSCCRES 447

BLAST of Cp4.1LG01g19460 vs. TAIR 10
Match: AT3G19970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 510.4 bits (1313), Expect = 1.5e-144
Identity = 269/442 (60.86%), Postives = 329/442 (74.43%), Query Frame = 0

Query: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQRPTEAASSSSFSDLSHNSTLDFLKES 60
           MGSLSGI+QRP +A  AV  AS+S+D S++  S R     S S     S +  +   +  
Sbjct: 1   MGSLSGIIQRPLVAAAAVITASVSADVSERFSSLRSLVRGSESEQIAPSVSGLVQEERSL 60

Query: 61  RTSFAFVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQSAAL 120
             S    SKL +LSFV++I  PV          SC    +L  S+ S + L ++YQSA L
Sbjct: 61  WVSQVSASKLADLSFVSRICVPVPNVDLLASNPSC----SLATSVTSLSALRSVYQSAEL 120

Query: 121 TKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQK 180
            KASK VA +   S    ++ YRWHLPEP+++D+SGSS+C   K+RTVVVLLGWLG+KQK
Sbjct: 121 AKASKPVAFTIGASLVVPDISYRWHLPEPSAVDMSGSSSCVSEKNRTVVVLLGWLGSKQK 180

Query: 181 HLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHGKNLVF 240
           HLK+YA+WYTS+G+H ITFT PM EI++YQ+ GK E++I+ LVNHLADWL+EE  KNLVF
Sbjct: 181 HLKKYADWYTSKGYHVITFTLPMNEIMSYQVGGKAEKNIESLVNHLADWLDEEQKKNLVF 240

Query: 241 HTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNS 300
           HTFSNTGWLTYGAILEKFQK DSS+MGR+KGCIVDSAPVAA DP VWASGFSAAFLKK+S
Sbjct: 241 HTFSNTGWLTYGAILEKFQKQDSSLMGRVKGCIVDSAPVAAADPTVWASGFSAAFLKKSS 300

Query: 301 VATKGLATLDSTGMEVPISSKEDIVPKPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVL 360
           VATKG A+       + IS      PKPA TE ALLLVLEK F V+L LP VNRRL+DVL
Sbjct: 301 VATKGSASSSYESNGINISQ-----PKPAATETALLLVLEKFFAVILNLPKVNRRLADVL 360

Query: 361 DTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDP 420
           DTLS++QPSCPQLYIYSSADRVIP + VESFI EQR+AG EVRACNF+S+PHVDHFR++P
Sbjct: 361 DTLSTAQPSCPQLYIYSSADRVIPVEQVESFIVEQRKAGHEVRACNFISSPHVDHFRSNP 420

Query: 421 KLYTSQLSQFLADCVLTCCRES 443
           +LYT++L+ F+ + VL+CC  S
Sbjct: 421 ELYTAELNHFMNNFVLSCCHHS 433

BLAST of Cp4.1LG01g19460 vs. TAIR 10
Match: AT2G18245.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 5.2e-65
Identity = 135/311 (43.41%), Postives = 190/311 (61.09%), Query Frame = 0

Query: 133 FSSPPSEVMYRWHLPEPTSLDISGSSACSVAK--SRTVVVLLGWLGAKQKHLKRYAEWYT 192
           F S P++ +  W+   P  ++  G++A         T VVLLGWLGAK KHL+RY EWY 
Sbjct: 88  FRSEPNDGVAVWN-RAPRIVNGGGNAAIFGGNEGKETTVVLLGWLGAKAKHLRRYVEWYN 147

Query: 193 SRGFHAITFTFPMAEILTYQLDGKVEQHIDLLVNHLADWLEEEHG----KNLVFHTFSNT 252
           SRG +A+TFT  + ++L   L  ++E+ I    N L +W+ E+      K LVFH+FSNT
Sbjct: 148 SRGINAVTFTVDVRDLLRLDLGRRLERRIAEFGNELVNWVSEKEDDGREKCLVFHSFSNT 207

Query: 253 GWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGL 312
           GWL YGA+LE F      ++ RIKGCI+DS      D ++WA+GF+AA LKK S      
Sbjct: 208 GWLVYGALLESFIGR-QDLVERIKGCIIDSGGADPLDTKIWAAGFTAAILKKRS------ 267

Query: 313 ATLDSTGMEVPISSKEDIVP---KPAVTEAALLLVLEKIFGVVLKLPTVNRRLSDVLDTL 372
            +  +T    PI  ++   P   +P   E  +L  LEK+F + L  P VN RL+ ++  L
Sbjct: 268 -STITTEPNSPIKEEDASTPQKKEPLGIENIMLSSLEKLFPIFLNHPDVNTRLTKIIQKL 327

Query: 373 SSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHFRNDPKLY 432
             + P CPQLY+YSS D+V+P+ SVE  I+EQ++ GR++ + NF S+PHVDH+RN P LY
Sbjct: 328 YENHPPCPQLYLYSSGDKVVPSHSVELRIREQQKIGRKIHSFNFKSSPHVDHYRNFPDLY 387

Query: 433 TSQLSQFLADC 435
           +SQL  FL +C
Sbjct: 388 SSQLQNFLQEC 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6DHN09.4e-1927.60Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1[more]
Q9D0Z31.2e-1830.43Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1[more]
Q6DJC83.9e-1726.33Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1[more]
Q6P2H88.8e-1729.63Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1[more]
Q2TBP51.1e-1628.31Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023552493.10.0100.00transmembrane protein 53-like [Cucurbita pepo subsp. pepo] >XP_023552501.1 trans... [more]
KAG6601980.13.26e-31399.10Transmembrane protein 53, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958510.11.33e-31298.87transmembrane protein 53-like [Cucurbita moschata] >XP_022958515.1 transmembrane... [more]
XP_022990028.16.03e-30997.74transmembrane protein 53-like [Cucurbita maxima] >XP_022990029.1 transmembrane p... [more]
XP_008465096.16.44e-27789.01PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_008465105.1 PREDICTED: tr... [more]
Match NameE-valueIdentityDescription
A0A6J1H3N46.42e-31398.87transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111459714 PE=3... [more]
A0A6J1JP032.92e-30997.74transmembrane protein 53-like OS=Cucurbita maxima OX=3661 GN=LOC111487044 PE=3 S... [more]
A0A1S3CN083.12e-27789.01transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103502788 PE=3 SV=1[more]
A0A0A0KMY91.47e-27388.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G352630 PE=3 SV=1[more]
A0A6J1CIU09.67e-26887.02transmembrane protein 53 OS=Momordica charantia OX=3673 GN=LOC111011995 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G19970.11.5e-14460.86alpha/beta-Hydrolases superfamily protein [more]
AT2G18245.15.2e-6543.41alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 143..436
e-value: 1.9E-10
score: 42.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 163..433
IPR008547Protein of unknown function DUF829, TMEM53PFAMPF05705DUF829coord: 167..431
e-value: 4.8E-71
score: 239.5
IPR008547Protein of unknown function DUF829, TMEM53PANTHERPTHR12265UNCHARACTERIZEDcoord: 57..442
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..48
NoneNo IPR availablePANTHERPTHR12265:SF30OS06G0730300 PROTEINcoord: 57..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g19460.1Cp4.1LG01g19460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity