Cp4.1LG01g18700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g18700
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	DUF2431 domain-containing protein
Location	Cp4.1LG01: 16061932 .. 16062901 (+)
RNA-Seq Expression	Cp4.1LG01g18700
Synteny	Cp4.1LG01g18700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCGTGGATCAGTAAGCGAGAAGACCATAATGCATTACAAAAGCTCCCATACCATACTTCTGGTCGGAGAAGGCGACTTCTCCTTCTCTGCATGCTTGGCCAAAGCCTTTGCCTCTGCTGCCAACATGGTTGCCACTTCCCTCGACTCGAAAGGTAGTCATAAGTTTTGATTTGATTTGGGTATTTGGATTAGATTTAGTTATATAATCACGTAAATATCTTAGTGTCTTTTTGTGTAATTCCGTTCATGGTGTTGTGAGCACATTTGTTCCGCTTTTGGCAAACAACGAGTGATGTTCAATGCTGTCCATTTTGAATATAAGCTTATGGCTTCTTGCTATTGAAAATGTATTATTTATTTATAAACTATGATCATTTACTTTCTCCCAATAATCTTCAATAGAATTGCTGCTGCGTAAGTACAGTCGTGTGGCTGCCAACTTAGAAGCGTTGGGGCTGCTTGGTGGCACGGTGTTGCATGAAGTGGATGCGACAGAGATGAGCCAACACTGCAGCCTCTCGTACAAGGAATTTGATCGTATGGTATTCAACTTCCCACATGCAGGGTTTTCTTTTCGAGAATCCGACCCTATTCAAATTAAGTATACCCCTTCCTTTTTAACTTATATTTATTTAATTATGCAAATTGTTATAAATAGAGAATTTTCACCATCATCCAGATGATTCGGGTATGATTGATTGACGGGTGTTTGTGATTACTTGTTTTGATAGACTCCACCAACATCTTGTGAGATGGTTTCTTAGGGAAGCAAGGAAATTGATGAGTGATAAAGGTGAAATTCACATAACCCACAAGATCTCATATCCTTATTATGATTGGGAGATTGAGGAATTGGCAAAAAAGGAGGGGCTGCTTCTGAATGAGAAGGCGGAGTTTAATCTGTGGGATTATCCAAATTATGAGAATAAAAGAGGGGGAGGTGGCAACAGCGACCTCACTTTCCCCGTC

mRNA sequence

ACCGTGGATCAGTAAGCGAGAAGACCATAATGCATTACAAAAGCTCCCATACCATACTTCTGGTCGGAGAAGGCGACTTCTCCTTCTCTGCATGCTTGGCCAAAGCCTTTGCCTCTGCTGCCAACATGGTTGCCACTTCCCTCGACTCGAAAGAATTGCTGCTGCGTAAGTACAGTCGTGTGGCTGCCAACTTAGAAGCGTTGGGGCTGCTTGGTGGCACGGTGTTGCATGAAGTGGATGCGACAGAGATGAGCCAACACTGCAGCCTCTCGTACAAGGAATTTGATCGTATGGTATTCAACTTCCCACATGCAGGGTTTTCTTTTCGAGAATCCGACCCTATTCAAATTAAACTCCACCAACATCTTGTGAGATGGTTTCTTAGGGAAGCAAGGAAATTGATGAGTGATAAAGGTGAAATTCACATAACCCACAAGATCTCATATCCTTATTATGATTGGGAGATTGAGGAATTGGCAAAAAAGGAGGGGCTGCTTCTGAATGAGAAGGCGGAGTTTAATCTGTGGGATTATCCAAATTATGAGAATAAAAGAGGGGGAGGTGGCAACAGCGACCTCACTTTCCCCGTC

Coding sequence (CDS)

ATGCATTACAAAAGCTCCCATACCATACTTCTGGTCGGAGAAGGCGACTTCTCCTTCTCTGCATGCTTGGCCAAAGCCTTTGCCTCTGCTGCCAACATGGTTGCCACTTCCCTCGACTCGAAAGAATTGCTGCTGCGTAAGTACAGTCGTGTGGCTGCCAACTTAGAAGCGTTGGGGCTGCTTGGTGGCACGGTGTTGCATGAAGTGGATGCGACAGAGATGAGCCAACACTGCAGCCTCTCGTACAAGGAATTTGATCGTATGGTATTCAACTTCCCACATGCAGGGTTTTCTTTTCGAGAATCCGACCCTATTCAAATTAAACTCCACCAACATCTTGTGAGATGGTTTCTTAGGGAAGCAAGGAAATTGATGAGTGATAAAGGTGAAATTCACATAACCCACAAGATCTCATATCCTTATTATGATTGGGAGATTGAGGAATTGGCAAAAAAGGAGGGGCTGCTTCTGAATGAGAAGGCGGAGTTTAATCTGTGGGATTATCCAAATTATGAGAATAAAAGAGGGGGAGGTGGCAACAGCGACCTCACTTTCCCCGTC

Protein sequence

MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLLGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNSDLTFPV

Homology

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BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 6.5e-46
Identity = 100/189 (52.91%), Postives = 124/189 (65.61%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY S+H ILLVGEG+FSFS CLA AF SA N+ ATSLDS++ L  KY     N+  L   
Sbjct: 10  HYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVDNINILKRY 69

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSF--RESDPIQIKLHQHLVRWFLR 121
           G  + HEVD   MS   SLS + +DR+VFNFPHAG  F  RE     I+ H+ LVR FL 
Sbjct: 70  GCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHKELVRGFLE 129

Query: 122 EARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGG 181
            A++++ + GEIHITHK +YP+ DW I++L K EGL L +K++F L  YP Y  KRG GG
Sbjct: 130 NAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPGYITKRGSGG 189

Query: 182 -NSDLTFPV 188
             SD  FPV
Sbjct: 190 RRSDDYFPV 198

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BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.1e-45
Identity = 91/184 (49.46%), Postives = 122/184 (66.30%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY S+H ILLVGEGDFSFS  LA  F SA+N+ A+SLDS ++++RKY +  +NL+ L  L
Sbjct: 23  HYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLKRL 82

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 121
           G  +LH VDAT +  H  L Y+ FDR++FNFPHAGF  RESD   I+ H+ LV  F   A
Sbjct: 83  GALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFNGA 142

Query: 122 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNS 181
            +L+   GE+H++HK   P+ +W +EELA +  L+L ++  F   +YP YENKRG G   
Sbjct: 143 SRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGRRC 202

Query: 182 DLTF 186
           D  F
Sbjct: 203 DQPF 206

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BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.0e-22
Identity = 62/174 (35.63%), Postives = 100/174 (57.47%), Query Frame = 0


Query: 3   YKSSHTILLVGEGDFSFSACLAKAFASAANMV-ATSLDSKELLLRKYSRVAANLEALGLL 62
           ++ ++  LL+GEG+FSF+  L     S+   V ATS DSKE L +KY   A  +  + + 
Sbjct: 54  FEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPDAAEYISKIEIN 113

Query: 63  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 122
           GG V+HE+DAT++  H  L  ++FD + +NFPH+G   ++ D   I  +Q ++  F + +
Sbjct: 114 GGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKDQDR-NILDNQKMLLAFFKAS 173

Query: 123 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKR 176
           + L+S+KG I IT   + PY  W ++ LAK  G       +F+   YP Y ++R
Sbjct: 174 KFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSHRR 226

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BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot
Match: P40493 (25S rRNA (uridine(2634)-N(3))-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BMT5 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.0e-10
Identity = 56/228 (24.56%), Postives = 99/228 (43.42%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKA-FASAANMVATSLDS--KELLLRKYSRVAANLEA 60
           M ++   T++L GEGDFSF+  + +  +  + N++ TS D+   EL L+       N + 
Sbjct: 66  MPFEKDETLMLCGEGDFSFARSIVEQNYIESDNLIITSYDNSVNELKLKYPHTFEENYQY 125

Query: 61  LGLLGGTVLHEVDATEMSQHCSLS------------------YKEFDRMVFNFPHAGFSF 120
           L  L   +  ++D T++ +   +S                   K    +VFNFPH G   
Sbjct: 126 LKDLNIPIFFQIDVTKLVKSFKISKNNTWFKIINRLSDHRWGNKPLQNIVFNFPHNGKGI 185

Query: 121 RESDPIQIKLHQHLVRWFLR-------------------------------EARKLMSD- 176
           ++ +   I+ HQ L+  F +                               +A+KL ++ 
Sbjct: 186 KDQER-NIREHQDLIFNFFQNSLQLFNLINTKIQNDTLRYTQGYDLNEDTPQAKKLTAEG 245

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BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-09
Identity = 49/176 (27.84%), Postives = 86/176 (48.86%), Query Frame = 0


Query: 9   ILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVA-ANLEALGLLGGTVLH 68
           +LLVGEG+FSF+A L      + ++ AT    +  L  +   VA  NL+ L   G  V  
Sbjct: 6   LLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAAL--EGDPVALENLKRLRERGVEVRF 65

Query: 69  EVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREARKLMSD 128
            VD T++S       ++FDR+ FNFPH G   R++    +  ++ L+  F +    +++ 
Sbjct: 66  GVDCTQLSHALPADDRDFDRIYFNFPHCG---RKAG---VAKNRELLAKFFQSCADVLAK 125

Query: 129 KGEIHIT----------HKISYPYYD-WEIEELAKKEGLLLNEKAEFNLWDYPNYE 173
            GE+H+T           K    +++ W++  +A   G +L++   F+    P Y+
Sbjct: 126 AGEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGYK 173

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BLAST of Cp4.1LG01g18700 vs. NCBI nr
Match: XP_023541512.1 (uncharacterized protein At4g26485-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 345 bits (886), Expect = 3.65e-119
Identity = 172/187 (91.98%), Postives = 172/187 (91.98%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL
Sbjct: 20  MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 79

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFP               LHQHLVRWFLRE
Sbjct: 80  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFP---------------LHQHLVRWFLRE 139

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN
Sbjct: 140 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 191

Query: 181 SDLTFPV 187
           SDLTFPV
Sbjct: 200 SDLTFPV 191

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BLAST of Cp4.1LG01g18700 vs. NCBI nr
Match: KAG7032604.1 (hypothetical protein SDJN02_06653, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 341 bits (875), Expect = 4.32e-117
Identity = 167/187 (89.30%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHYKSSHTILLVGEGDFSFSACLAKAF SA NMVATSLDS E+LLRKYSRVAANLEALGL
Sbjct: 20  MHYKSSHTILLVGEGDFSFSACLAKAFGSATNMVATSLDSNEVLLRKYSRVAANLEALGL 79

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDAT MS+HCSL YKEFDR+VFNFPHAGFSFRE+D IQIKLHQ LVR FLRE
Sbjct: 80  LGGTVLHEVDATAMSRHCSLCYKEFDRIVFNFPHAGFSFRETDAIQIKLHQDLVRGFLRE 139

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           ARKL+SDKGEIHITHKISYPY +WEIEELAKKEGLLL E AEF+LWDYPNYENKRG GGN
Sbjct: 140 ARKLVSDKGEIHITHKISYPYCEWEIEELAKKEGLLLKETAEFSLWDYPNYENKRGEGGN 199

Query: 181 SDLTFPV 187
           SD TFPV
Sbjct: 200 SDHTFPV 206

[Close]

BLAST of Cp4.1LG01g18700 vs. NCBI nr
Match: XP_022959460.1 (uncharacterized protein At4g26485-like [Cucurbita moschata])

HSP 1 Score: 336 bits (861), Expect = 4.43e-115
Identity = 163/187 (87.17%), Postives = 173/187 (92.51%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHYKSSHTILLVGEGDFSFSACLAKAF SAANMVATSLDS E+LLRKYSRVAANLEALGL
Sbjct: 20  MHYKSSHTILLVGEGDFSFSACLAKAFGSAANMVATSLDSNEVLLRKYSRVAANLEALGL 79

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDAT MS+HCSL YKEFDR++FNFPHAGFSF ESD +QIKLHQ LVR FLRE
Sbjct: 80  LGGTVLHEVDATAMSRHCSLCYKEFDRIIFNFPHAGFSFPESDAVQIKLHQDLVRGFLRE 139

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           ARKL+SDKGEIHITHKIS+PY +WEIE+LAKKEGLLL E AEF+ WDYPNYENKRGGGGN
Sbjct: 140 ARKLVSDKGEIHITHKISHPYCEWEIEKLAKKEGLLLKETAEFSRWDYPNYENKRGGGGN 199

Query: 181 SDLTFPV 187
           SD  FPV
Sbjct: 200 SDHAFPV 206

[Close]

BLAST of Cp4.1LG01g18700 vs. NCBI nr
Match: XP_022139691.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 265 bits (676), Expect = 1.36e-86
Identity = 129/186 (69.35%), Postives = 149/186 (80.11%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY SSHTILLVGEGDFSFS CLA  F SAANMVATSLDSKE LL KY+ VA NL+ L  L
Sbjct: 15  HYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELEEL 74

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 121
           G TV H+VDAT MSQH  L +K FDR+VFNFPHAGF F+ESD  QI+LH+HLVR FLR A
Sbjct: 75  GCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLRNA 134

Query: 122 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNS 181
           ++ + ++GEIHITHK ++P+  WEI ELA++EGLLL E+  F LW+YPNYENKRG G NS
Sbjct: 135 KEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGWNS 194

Query: 182 DLTFPV 187
           D TFPV
Sbjct: 195 DGTFPV 200

[Close]

BLAST of Cp4.1LG01g18700 vs. NCBI nr
Match: XP_011659737.1 (uncharacterized protein At4g26485 [Cucumis sativus] >KGN44112.1 hypothetical protein Csa_016883 [Cucumis sativus])

HSP 1 Score: 242 bits (618), Expect = 3.18e-78
Identity = 120/187 (64.17%), Postives = 143/187 (76.47%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHY SSH ILLVGEGDFSFSACLA AFASA+N++ATSLDS++ L+ KY+R A N++ L  
Sbjct: 11  MHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRDELVMKYARAARNVKILEE 70

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDAT MSQH  L    FDR+VFNFPHAGF ++ES+  QI+LH++LVR FLR 
Sbjct: 71  LGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKESNTAQIELHRNLVRVFLRN 130

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           A+ ++  +GEIHITHK S+P+  W I  LA  EGL L E   F+ W YPNYENKRG G N
Sbjct: 131 AKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEIFHAWQYPNYENKRGHGQN 190

Query: 181 SDLTFPV 187
            D TFPV
Sbjct: 191 PDGTFPV 197

[Close]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL
Match: A0A6J1H6C3 (uncharacterized protein At4g26485-like OS=Cucurbita moschata OX=3662 GN=LOC111460425 PE=4 SV=1)

HSP 1 Score: 336 bits (861), Expect = 2.14e-115
Identity = 163/187 (87.17%), Postives = 173/187 (92.51%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHYKSSHTILLVGEGDFSFSACLAKAF SAANMVATSLDS E+LLRKYSRVAANLEALGL
Sbjct: 20  MHYKSSHTILLVGEGDFSFSACLAKAFGSAANMVATSLDSNEVLLRKYSRVAANLEALGL 79

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDAT MS+HCSL YKEFDR++FNFPHAGFSF ESD +QIKLHQ LVR FLRE
Sbjct: 80  LGGTVLHEVDATAMSRHCSLCYKEFDRIIFNFPHAGFSFPESDAVQIKLHQDLVRGFLRE 139

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           ARKL+SDKGEIHITHKIS+PY +WEIE+LAKKEGLLL E AEF+ WDYPNYENKRGGGGN
Sbjct: 140 ARKLVSDKGEIHITHKISHPYCEWEIEKLAKKEGLLLKETAEFSRWDYPNYENKRGGGGN 199

Query: 181 SDLTFPV 187
           SD  FPV
Sbjct: 200 SDHAFPV 206

[Close]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL
Match: A0A6J1CDH1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010537 PE=4 SV=1)

HSP 1 Score: 265 bits (676), Expect = 6.56e-87
Identity = 129/186 (69.35%), Postives = 149/186 (80.11%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY SSHTILLVGEGDFSFS CLA  F SAANMVATSLDSKE LL KY+ VA NL+ L  L
Sbjct: 15  HYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELEEL 74

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 121
           G TV H+VDAT MSQH  L +K FDR+VFNFPHAGF F+ESD  QI+LH+HLVR FLR A
Sbjct: 75  GCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLRNA 134

Query: 122 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNS 181
           ++ + ++GEIHITHK ++P+  WEI ELA++EGLLL E+  F LW+YPNYENKRG G NS
Sbjct: 135 KEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGWNS 194

Query: 182 DLTFPV 187
           D TFPV
Sbjct: 195 DGTFPV 200

[Close]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL
Match: A0A0A0K734 (DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=4 SV=1)

HSP 1 Score: 242 bits (618), Expect = 1.54e-78
Identity = 120/187 (64.17%), Postives = 143/187 (76.47%), Query Frame = 0


Query: 1   MHYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGL 60
           MHY SSH ILLVGEGDFSFSACLA AFASA+N++ATSLDS++ L+ KY+R A N++ L  
Sbjct: 11  MHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRDELVMKYARAARNVKILEE 70

Query: 61  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 120
           LGGTVLHEVDAT MSQH  L    FDR+VFNFPHAGF ++ES+  QI+LH++LVR FLR 
Sbjct: 71  LGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKESNTAQIELHRNLVRVFLRN 130

Query: 121 ARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 180
           A+ ++  +GEIHITHK S+P+  W I  LA  EGL L E   F+ W YPNYENKRG G N
Sbjct: 131 AKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEIFHAWQYPNYENKRGHGQN 190

Query: 181 SDLTFPV 187
            D TFPV
Sbjct: 191 PDGTFPV 197

[Close]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL
Match: A0A6J1IKX9 (uncharacterized protein At4g26485 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476560 PE=4 SV=1)

HSP 1 Score: 242 bits (618), Expect = 2.98e-78
Identity = 120/188 (63.83%), Postives = 147/188 (78.19%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKY-SRVAANLEALGL 61
           HY SSH ILLVGEG+FSFSACLA AF +A NMVATSLDS+E LLRKY S +   LE L  
Sbjct: 11  HYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSDIKTTLEELKE 70

Query: 62  LGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSF-RESDPIQIKLHQHLVRWFLR 121
           LG +V+H VD   MSQH  L +  FDR+VFNFPHAGF + RE +  QIKLHQ+LVR FLR
Sbjct: 71  LGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKLHQNLVRSFLR 130

Query: 122 EARKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGG 181
            A++L+++ GEIHITHKISYPY +W+IE++A++EGL L E+ EF+ WDYP Y+NK+G G 
Sbjct: 131 NAKELLAENGEIHITHKISYPYSEWKIEKIAEEEGLFLREEVEFDKWDYPCYDNKKGSGP 190

Query: 182 NSDLTFPV 187
           NS+ TFPV
Sbjct: 191 NSNRTFPV 198

[Close]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL
Match: A0A5D3DIB6 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G002400 PE=4 SV=1)

HSP 1 Score: 238 bits (608), Expect = 4.47e-77
Identity = 120/186 (64.52%), Postives = 140/186 (75.27%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY SSH ILLVGEGDFSFSACLA AFASA+N+VATSLDS + L+ KY+    NL+ L  L
Sbjct: 11  HYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHANRNLKILEEL 70

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 121
           GG VLH+VDAT MSQH  L    FDR+VFNFPHAGF + E +  QI+LH+ LVR FLR A
Sbjct: 71  GGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRDLVRGFLRNA 130

Query: 122 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNS 181
           ++++S  GEIHITHK S+P+  WEI +LA  EGL L E AEF  W YPNYENKRG G NS
Sbjct: 131 KRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYENKRGDGWNS 190

Query: 182 DLTFPV 187
           + TFPV
Sbjct: 191 NGTFPV 196

[Close]

BLAST of Cp4.1LG01g18700 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 184.5 bits (467), Expect = 7.8e-47
Identity = 91/184 (49.46%), Postives = 122/184 (66.30%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY S+H ILLVGEGDFSFS  LA  F SA+N+ A+SLDS ++++RKY +  +NL+ L  L
Sbjct: 23  HYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLKRL 82

Query: 62  GGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLREA 121
           G  +LH VDAT +  H  L Y+ FDR++FNFPHAGF  RESD   I+ H+ LV  F   A
Sbjct: 83  GALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFNGA 142

Query: 122 RKLMSDKGEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGNS 181
            +L+   GE+H++HK   P+ +W +EELA +  L+L ++  F   +YP YENKRG G   
Sbjct: 143 SRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGRRC 202

Query: 182 DLTF 186
           D  F
Sbjct: 203 DQPF 206

[Close]

BLAST of Cp4.1LG01g18700 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 172.2 bits (435), Expect = 4.0e-43
Identity = 94/185 (50.81%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 3   YKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLLG 62
           Y +   ILLVGEGDFSFS  LA+ F SA N+ ATSLD++E L  KY+   AN+E L L G
Sbjct: 12  YSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLELFG 71

Query: 63  GTVLHEVDATEMSQHCSLSYKEFDRMVFNFPHAGFSF-RESDPIQIKLHQHLVRWFLREA 122
            TV+H V+   MS    L    +DR++FNFPH+G  F  E D   I LHQ LVR FL  A
Sbjct: 72  CTVVHGVNVHSMSSDYRLG--RYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFLESA 131

Query: 123 RKLMSDK-GEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGGGGN 182
           RK++ D+ GEIH+THK + P+  W IE LA ++GL L  + EF+ W +P Y NK+GGG N
Sbjct: 132 RKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGGGSN 191

Query: 183 SDLTF 186
            + TF
Sbjct: 192 CNSTF 194

[Close]

BLAST of Cp4.1LG01g18700 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 146.0 bits (367), Expect = 3.1e-35
Identity = 84/190 (44.21%), Postives = 114/190 (60.00%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKELLLRKYSRVAANLEALGLL 61
           HY +   ILLVGEGDFSFS  LA+AF SA+N+ ATSLD++  L +K+    AN+E L  L
Sbjct: 69  HYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNGKANVEELERL 128

Query: 62  GGTVLHEVDATEMSQHCSLSYKE-FDRMVFNFPHAGFSFRESDPIQIKLHQHLVRWFLRE 121
           G +V++ V+   M    S+     +DR++FNFP                   LVR F++ 
Sbjct: 129 GCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP----------------THELVRGFMKS 188

Query: 122 ARKLMSDK---GEIHITHKISYPYYDWEIEELAKKEGLLLNEKAEFNLWDYPNYENKRGG 181
           AR L+ D+   GEIH+ HK  YP+ +W+++ L +KEGL L  + EF L  YP Y NKRG 
Sbjct: 189 ARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHYPGYFNKRGS 242

Query: 182 GGNSDLTFPV 188
           GG SD +FPV
Sbjct: 249 GGYSDSSFPV 242

[Close]

BLAST of Cp4.1LG01g18700 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-34
Identity = 78/159 (49.06%), Postives = 100/159 (62.89%), Query Frame = 0


Query: 32  NMVATSLDSKELLLRKYSRVAANLEALGLLGGTVLHEVDATEMSQHCSLSYKEFDRMVFN 91
           N+ ATSLDS++ L  KY     N+  L   G  + HEVD   MS   SLS + +DR+VFN
Sbjct: 2   NITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFN 61

Query: 92  FPHAGFSF--RESDPIQIKLHQHLVRWFLREARKLMSDKGEIHITHKISYPYYDWEIEEL 151
           FPHAG  F  RE     I+ H+ LVR FL  A++++ + GEIHITHK +YP+ DW I++L
Sbjct: 62  FPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKL 121

Query: 152 AKKEGLLLNEKAEFNLWDYPNYENKRGGGG-NSDLTFPV 188
            K EGL L +K++F L  YP Y  KRG GG  SD  FPV
Sbjct: 122 GKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPV 160

[Close]

BLAST of Cp4.1LG01g18700 vs. TAIR 10
Match: AT1G55800.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 121.7 bits (304), Expect = 6.2e-28
Identity = 79/215 (36.74%), Postives = 100/215 (46.51%), Query Frame = 0


Query: 2   HYKSSHTILLVGEGDFSFSACLAKAFASAANMVATSLDSKEL------------------ 61
           HY S H ILLVGEGDFSFS  LA  F SA+N+ A+SLDS +                   
Sbjct: 21  HYSSKHQILLVGEGDFSFSCSLATCFGSASNIYASSLDSYDYKPVNKGCSFKLDFLSCCM 80

Query: 62  ---------LLRKYSRVAANLEALGLLGGTVLHEVDATEMSQHCSLSYKEFDRMVFNFPH 121
                    ++RKY    +NLE L  LG  +LH VDAT +  H  L Y+ FDR++FNFPH
Sbjct: 81  SFMVIKPDDVVRKYKNARSNLETLKRLGAFLLHGVDATTLHFHPDLRYRRFDRVIFNFPH 140

Query: 122 AGFSFRESDPIQIK----LHQHLVRWFLREARKLMSDKGEIHITHKISYPYYDWEIEELA 181
            GF  +ESDP QI+      ++L + FL  A  ++   GE+                   
Sbjct: 141 TGFHRKESDPCQIQPAAATLRNLFKDFLHGASHMLRADGELE------------------ 200

Query: 182 KKEGLLLNEKAEFNLWDYPNYENKRGGGGNSDLTF 186
                       F   +YP YENKRG G   D  F
Sbjct: 201 -----------AFEKRNYPGYENKRGDGSRCDQPF 206

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g18700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C8L4	6.5e-46	52.91	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
F4I1X0	1.1e-45	49.46	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
O94480	5.0e-22	35.63	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
P40493	2.0e-10	24.56	25S rRNA (uridine(2634)-N(3))-methyltransferase OS=Saccharomyces cerevisiae (str...	[more]
Q3UY23	1.3e-09	27.84	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]

BLAST of Cp4.1LG01g18700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541512.1	3.65e-119	91.98	uncharacterized protein At4g26485-like [Cucurbita pepo subsp. pepo]	[more]
KAG7032604.1	4.32e-117	89.30	hypothetical protein SDJN02_06653, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022959460.1	4.43e-115	87.17	uncharacterized protein At4g26485-like [Cucurbita moschata]	[more]
XP_022139691.1	1.36e-86	69.35	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
XP_011659737.1	3.18e-78	64.17	uncharacterized protein At4g26485 [Cucumis sativus] >KGN44112.1 hypothetical pro...	[more]

BLAST of Cp4.1LG01g18700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6C3	2.14e-115	87.17	uncharacterized protein At4g26485-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CDH1	6.56e-87	69.35	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0K734	1.54e-78	64.17	DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=...	[more]
A0A6J1IKX9	2.98e-78	63.83	uncharacterized protein At4g26485 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5D3DIB6	4.47e-77	64.52	DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Cp4.1LG01g18700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	7.8e-47	49.46	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	4.0e-43	50.81	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	3.1e-35	44.21	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	3.4e-34	49.06	Domain of unknown function (DUF2431)	[more]
AT1G55800.1	6.2e-28	36.74	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 10..175 e-value: 3.2E-49 score: 167.4
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 1..187
None	No IPR available	PANTHER	PTHR11538:SF80	SUBFAMILY NOT NAMED	coord: 1..187
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 6..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g18700.1	Cp4.1LG01g18700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity