Cp4.1LG01g17730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g17730 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.0e-55
Identity = 156/461 (33.84%), Postives = 232/461 (50.33%), Query Frame = 0

Query: 21  LLLSSLTDPSNAIPSQYLKFPLLHTNPFSS---PSQALSS----DTHRLSLLFS------ 80
           LL S     S++  S  +   L H +  SS   P +  SS    D+ R+  + +      
Sbjct: 55  LLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIP 114

Query: 81  --SHRHSPT---LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCR 140
             +  H+P      S ++SG S GSG+YF  L +GTP + + +V DTGSD+VW++C+PCR
Sbjct: 115 GRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCR 174

Query: 141 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSL 200
            C +      F PR S +++   C  PHCR L    S  CN       C +  SY D S 
Sbjct: 175 RC-YSQSDPIFDPRKSKTYATIPCSSPHCRRL---DSAGCNTR--RKTCLYQVSYGDGSF 234

Query: 201 SSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPIS 260
           + G FS +  TF        + R+  ++ GCG    G       F GA G++GLG+G +S
Sbjct: 235 TVGDFSTETLTFR-------RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLS 294

Query: 261 FSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPT 320
           F  Q G RF   FSYCL+D + S  P+S +     +  +         +TPL  NP   T
Sbjct: 295 FPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDT 354

Query: 321 FYYIVVKSITVDGVKLP-INPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRR 380
           FYY+ +  I+V G ++P +  +++ +D+ GNGG ++DSGT++T L   AY  +  A R  
Sbjct: 355 FYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVG 414

Query: 381 VK-LPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETE-EGVMC 440
            K L RA   S  FD C + S  + ++ +P +     G  V + PA NY +  +  G  C
Sbjct: 415 AKTLKRAPDFSL-FDTCFDLSNMNEVK-VPTVVLHFRGADV-SLPATNYLIPVDTNGKFC 474

Query: 441 LAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGC 461
            A     +  G S+IGN+ QQGF + +D   SR+GF+  GC
Sbjct: 475 FAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Cp4.1LG01g17730 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.0e-52
Identity = 135/409 (33.01%), Postives = 207/409 (50.61%), Query Frame = 0

Query: 53  QALSSDTHRLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSD 112
           +A+     R+  + +  + S  +++P+ +    G G+Y +N+ +GTP  S   + DTGSD
Sbjct: 63  RAIKRGERRMRSINAMLQSSSGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSD 122

Query: 113 LVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCP 172
           L+W +C PC  C    P+  F P+ SSSFS   C   +C+ L   PS  CN    ++ C 
Sbjct: 123 LIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLPCESQYCQDL---PSETCN----NNECQ 182

Query: 173 FLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARG 232
           + Y Y D S + G+ + +  TF T S       + +++FGCG    G      + NGA G
Sbjct: 183 YTYGYGDGSTTQGYMATETFTFETSS-------VPNIAFGCGEDNQG----FGQGNGA-G 242

Query: 233 VMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYT 292
           ++G+G GP+S  SQLG      FSYC+  Y  S P T  L +G     +P  + S    T
Sbjct: 243 LIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSPST--LALGSAASGVPEGSPS----T 302

Query: 293 PLQINPLSPTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYE 352
            L  + L+PT+YYI ++ ITV G  L I  + + + + G GG ++DSGTTLTYL + AY 
Sbjct: 303 TLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYN 362

Query: 353 EVLKAMRRRVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVE 412
            V +A   ++ LP   + S G   C     +     +P++  +   GGV     +N  + 
Sbjct: 363 AVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQF-DGGVLNLGEQNILIS 422

Query: 413 TEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCG 462
             EGV+CLA+    S  G S+ GN+ QQ   + +D     + F    CG
Sbjct: 423 PAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCG 436

BLAST of Cp4.1LG01g17730 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.8e-52
Identity = 130/377 (34.48%), Postives = 192/377 (50.93%), Query Frame = 0

Query: 86  GSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFH 145
           G G+Y +NL +GTP Q    + DTGSDL+W +C PC  C  +  +  F P+ SSSFS   
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLP 150

Query: 146 CFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTR 205
           C    C+ L  P       T  ++ C + Y Y D S + G    +  TF + S       
Sbjct: 151 CSSQLCQALSSP-------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVS------- 210

Query: 206 LNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLS 265
           + +++FGCG    G      + NGA G++G+GRGP+S  SQL       FSYC+     S
Sbjct: 211 IPNITFGCGENNQG----FGQGNGA-GLVGMGRGPLSLPSQLD---VTKFSYCMTPIGSS 270

Query: 266 PPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPNVW 325
            P  S L++G    S+    A   + T +Q + + PTFYYI +  ++V   +LPI+P+ +
Sbjct: 271 TP--SNLLLGSLANSV---TAGSPNTTLIQSSQI-PTFYYITLNGLSVGSTRLPIDPSAF 330

Query: 326 AID-EQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASGES 385
           A++   G GG ++DSGTTLTY    AY+ V +    ++ LP     S GFDLC     + 
Sbjct: 331 ALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDP 390

Query: 386 RMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLL 445
               +P       GG +   P+ NYF+    G++CLA+    S  G S+ GN+ QQ  L+
Sbjct: 391 SNLQIPTFVMHFDGGDL-ELPSENYFISPSNGLICLAMG--SSSQGMSIFGNIQQQNMLV 435

Query: 446 EFDRDKSRMGFSRRGCG 462
            +D   S + F+   CG
Sbjct: 451 VYDTGNSVVSFASAQCG 435

BLAST of Cp4.1LG01g17730 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 129/386 (33.42%), Postives = 195/386 (50.52%), Query Frame = 0

Query: 77  SPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPR 136
           S ++SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C PC+ C +      F P 
Sbjct: 118 SDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPA 177

Query: 137 HSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNT 196
            S S++   C    C  + +   H          C +   Y D S + G  + +  TF  
Sbjct: 178 KSGSYTGVSCGSSVCDRIENSGCH-------SGGCRYEVMYGDGSYTKGTLALETLTF-- 237

Query: 197 FSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFS 256
                 +T + +++ GCG R  G       F GA G++G+G G +SF  QL  + G  F 
Sbjct: 238 -----AKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFG 297

Query: 257 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGV 316
           YCL+  +     T  L+   G  +LPV      S+ PL  NP +P+FYY+ +K + V GV
Sbjct: 298 YCLV--SRGTDSTGSLVF--GREALPV----GASWVPLVRNPRAPSFYYVGLKGLGVGGV 357

Query: 317 KLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMR-RRVKLPRALQLSPGFD 376
           ++P+   V+ + E G+GG V+D+GT +T L  AAY       + +   LPRA  +S  FD
Sbjct: 358 RIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FD 417

Query: 377 LCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEE-GVMCLAIRPVDSGNGFSVI 436
            C + SG   +R +P + F    G V   PARN+ +  ++ G  C A     S  G S+I
Sbjct: 418 TCYDLSGFVSVR-VPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF--AASPTGLSII 470

Query: 437 GNLMQQGFLLEFDRDKSRMGFSRRGC 461
           GN+ Q+G  + FD     +GF    C
Sbjct: 478 GNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Cp4.1LG01g17730 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.3e-47
Identity = 125/391 (31.97%), Postives = 195/391 (49.87%), Query Frame = 0

Query: 75  LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFF 134
           L +P++SGAS GSG+YF  + +GTP + + LV DTGSD+ W++C PC +C +      F 
Sbjct: 147 LTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADC-YQQSDPVFN 206

Query: 135 PRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTF 194
           P  SS++    C  P C LL    +  C      + C +  SY D S + G  + D  TF
Sbjct: 207 PTSSSTYKSLTCSAPQCSLL---ETSACR----SNKCLYQVSYGDGSFTVGELATDTVTF 266

Query: 195 NTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNT 254
              SG     ++N+++ GCG    G       F GA G++GLG G +S ++Q+      +
Sbjct: 267 GN-SG-----KINNVALGCGHDNEG------LFTGAAGLLGLGGGVLSITNQMK---ATS 326

Query: 255 FSYCLMDYTLSPPPT---SYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSI 314
           FSYCL+D       +   + + +GGG  + P+    KI            TFYY+ +   
Sbjct: 327 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKID-----------TFYYVGLSGF 386

Query: 315 TVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA-MRRRVKLPRALQL 374
           +V G K+ +   ++ +D  G+GG ++D GT +T L   AY  +  A ++  V L +    
Sbjct: 387 SVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSS 446

Query: 375 SPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEE-GVMCLAIRPVDSGN 434
              FD C + S  S ++ +P + F   GG     PA+NY +  ++ G  C A  P  S  
Sbjct: 447 ISLFDTCYDFSSLSTVK-VPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS-- 500

Query: 435 GFSVIGNLMQQGFLLEFDRDKSRMGFSRRGC 461
             S+IGN+ QQG  + +D  K+ +G S   C
Sbjct: 507 SLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Cp4.1LG01g17730 vs. NCBI nr
Match: XP_023549731.1 (aspartic proteinase nepenthesin-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60
           MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH
Sbjct: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP
Sbjct: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463

BLAST of Cp4.1LG01g17730 vs. NCBI nr
Match: KAG6601756.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 448/463 (96.76%), Postives = 454/463 (98.06%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60
           MPCVSQFLHLTF     LLLLLLSSL D SNAIPSQYLKFPLLH+NPFSSPSQALSSDTH
Sbjct: 1   MPCVSQFLHLTF-----LLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFS+HRHSP LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP
Sbjct: 61  RLSLLFSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPS+RCNHTHLHSPCPFLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNASGESRMRSLP++RFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           AIRPVDSGNGFSVIGNLMQQGFLLEFDR+KSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458

BLAST of Cp4.1LG01g17730 vs. NCBI nr
Match: KAG7032480.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 447/463 (96.54%), Postives = 453/463 (97.84%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60
           M CVSQFLHLTF     LLLLLLSSL D SNAIPSQYLKFPLLH+NPFSSPSQALSSDTH
Sbjct: 1   MACVSQFLHLTF-----LLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFS+HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP
Sbjct: 61  RLSLLFSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPS SGARFNGARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSFSGARFNGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITV+GVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVNGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNASGESRMRSLP++RFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           AIRPVDSGNGFSVIGNLMQQGFLLEFDR+KSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458

BLAST of Cp4.1LG01g17730 vs. NCBI nr
Match: XP_022930101.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 443/463 (95.68%), Postives = 446/463 (96.33%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60
           MPCV QFL        LLLLLLLSSL D SNAIPSQYLKFPLLHTNPFSSPSQALSSDTH
Sbjct: 1   MPCVPQFL--------LLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFS+HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLV DTGSDLVWVKCSP
Sbjct: 61  RLSLLFSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPC FLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARF GARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLG RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNAS ESRMRSLPQ+RFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           AIRPVDSGNGFSVIGNLMQQGFLLEFDR+KSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Cp4.1LG01g17730 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 748 bits (1931), Expect = 1.71e-269
Identity = 379/466 (81.33%), Postives = 405/466 (86.91%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPS-QYLKFPLLHTNPFSSPSQALSSDT 60
           M  VS F HL         +LLL SL D  NA  +  YLK PLLH NPFSSPSQALSSDT
Sbjct: 5   MLSVSTFFHL---------ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDT 64

Query: 61  HRLSLLFSS--HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVK 120
           HRLSLLFS+   R +PTLKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVK
Sbjct: 65  HRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVK 124

Query: 121 CSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSY 180
           CS CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLY+Y
Sbjct: 125 CSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTY 184

Query: 181 ADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLG 240
           AD S SSGFFSK+ TT  T SG+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLG
Sbjct: 185 ADGSTSSGFFSKETTTLKTLSGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLG 244

Query: 241 RGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQIN 300
           RGPISFS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNA+KISYTPL IN
Sbjct: 245 RGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLIN 304

Query: 301 PLSPTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA 360
           PLSPTFYYI VKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKA
Sbjct: 305 PLSPTFYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA 364

Query: 361 MRRRVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGV 420
           +R+RVKLP A +L+PGFDLCVN S ES+  SLP++RFRVG G VFAPPARNYF+ET EGV
Sbjct: 365 VRQRVKLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGV 424

Query: 421 MCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           MCLAIR V+ GNGFSVIGNLMQQGFLLEFD++ SR+GFSRRGCGLP
Sbjct: 425 MCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Cp4.1LG01g17730 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 443/463 (95.68%), Postives = 446/463 (96.33%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60
           MPCV QFL        LLLLLLLSSL D SNAIPSQYLKFPLLHTNPFSSPSQALSSDTH
Sbjct: 1   MPCVPQFL--------LLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFS+HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLV DTGSDLVWVKCSP
Sbjct: 61  RLSLLFSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPC FLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARF GARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLG RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNAS ESRMRSLPQ+RFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           AIRPVDSGNGFSVIGNLMQQGFLLEFDR+KSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Cp4.1LG01g17730 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 8.28e-270
Identity = 379/466 (81.33%), Postives = 405/466 (86.91%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPS-QYLKFPLLHTNPFSSPSQALSSDT 60
           M  VS F HL         +LLL SL D  NA  +  YLK PLLH NPFSSPSQALSSDT
Sbjct: 5   MLSVSTFFHL---------ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDT 64

Query: 61  HRLSLLFSS--HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVK 120
           HRLSLLFS+   R +PTLKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVK
Sbjct: 65  HRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVK 124

Query: 121 CSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSY 180
           CS CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLY+Y
Sbjct: 125 CSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTY 184

Query: 181 ADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLG 240
           AD S SSGFFSK+ TT  T SG+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLG
Sbjct: 185 ADGSTSSGFFSKETTTLKTLSGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLG 244

Query: 241 RGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQIN 300
           RGPISFS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNA+KISYTPL IN
Sbjct: 245 RGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLIN 304

Query: 301 PLSPTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA 360
           PLSPTFYYI VKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKA
Sbjct: 305 PLSPTFYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA 364

Query: 361 MRRRVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGV 420
           +R+RVKLP A +L+PGFDLCVN S ES+  SLP++RFRVG G VFAPPARNYF+ET EGV
Sbjct: 365 VRQRVKLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGV 424

Query: 421 MCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           MCLAIR V+ GNGFSVIGNLMQQGFLLEFD++ SR+GFSRRGCGLP
Sbjct: 425 MCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Cp4.1LG01g17730 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 744 bits (1921), Expect = 2.76e-268
Identity = 375/466 (80.47%), Postives = 404/466 (86.70%), Query Frame = 0

Query: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPS-QYLKFPLLHTNPFSSPSQALSSDT 60
           M  VS F HL         +LLL SL D  NA  +  YLK PLLH NPFSSPSQALSSDT
Sbjct: 5   MLSVSPFFHL---------ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDT 64

Query: 61  HRLSLLFSS--HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVK 120
           HRLSLLFS+   R +PTLKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVK
Sbjct: 65  HRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVK 124

Query: 121 CSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSY 180
           CS CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLY+Y
Sbjct: 125 CSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTY 184

Query: 181 ADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLG 240
           AD S SSGFFSK+ TT  T +G+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLG
Sbjct: 185 ADGSTSSGFFSKETTTLKTLTGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLG 244

Query: 241 RGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQIN 300
           RGPISFS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLR LPVTNA+KISYTPL IN
Sbjct: 245 RGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLIN 304

Query: 301 PLSPTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA 360
           PLSPTFYYI VKSITVDGVKLPINP +WAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKA
Sbjct: 305 PLSPTFYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA 364

Query: 361 MRRRVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGV 420
           MR+RVKLP A +L+PGFDLCVN S ES+  SLP++RF++G G VF PPARNYF+ETEEGV
Sbjct: 365 MRQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGV 424

Query: 421 MCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           MCLAIR V+ GNGFSVIGNLMQQGFLLEFD++ SR+GFSRRGCGLP
Sbjct: 425 MCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Cp4.1LG01g17730 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 739 bits (1908), Expect = 2.63e-266
Identity = 368/455 (80.88%), Postives = 402/455 (88.35%), Query Frame = 0

Query: 12  FFLLLLLLLLLLSSLTDPSN---AIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSS 71
           FFLL+LL    L+ L +P+    A P+ +LK PLLH  PFSSPSQ+LSSDTHRLSLLFS 
Sbjct: 9   FFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS- 68

Query: 72  HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHP 131
            R +PTLKSPLISGASTGSGQYFV++ LGTPPQSLLLVADTGSDLVWVKCS CRNCSHHP
Sbjct: 69  -RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHP 128

Query: 132 PSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSGFFS 191
           PSSAF PRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLYSYAD SLSSGFFS
Sbjct: 129 PSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFS 188

Query: 192 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 251
           K+ TT  + SG+  +  L  LSFGCGFRISGPSVSGA+FNGARGVMGLGRG ISFSSQLG
Sbjct: 189 KETTTLKSLSGS--EIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 248

Query: 252 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 311
           RRFGN FSYCLMDYTLSPPPTS+LMIGGGL SLP+TNA+KISYTPLQINPLSPTFYYI +
Sbjct: 249 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITI 308

Query: 312 KSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 371
            SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP A 
Sbjct: 309 HSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 368

Query: 372 QLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSG 431
           +L+PGFDLCVNASGESR  SLP+LRFR+GGG VFAPP RNYF+ETEEGVMCLAIR V+SG
Sbjct: 369 ELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESG 428

Query: 432 NGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           NGFSVIGNLMQQGFLLEFD+++SR+GF+RRGCGLP
Sbjct: 429 NGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Cp4.1LG01g17730 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 738 bits (1905), Expect = 7.53e-266
Identity = 368/455 (80.88%), Postives = 401/455 (88.13%), Query Frame = 0

Query: 12  FFLLLLLLLLLLSSLTDPSN---AIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSS 71
           FFLL+ L    L+ L++P+    A  + +LK PLLH  PFSSPSQ+LSSDTHRLSLLFS 
Sbjct: 9   FFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS- 68

Query: 72  HRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHP 131
            R +PTLKSPLISGASTGSGQYFV++ LGTPPQSLLLVADTGSDLVWVKCS CRNCSHHP
Sbjct: 69  -RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHP 128

Query: 132 PSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSGFFS 191
           PSSAFFPRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLYSYAD SLSSGFFS
Sbjct: 129 PSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFS 188

Query: 192 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 251
           K+ TT  T SG+  +  L  LSFGCGFRISGPSVSGA+FNGARGVMGLGRG ISFSSQLG
Sbjct: 189 KETTTLKTLSGS--EIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLG 248

Query: 252 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 311
           RRFGN FSYCLMDYTLSPPPTS+LMIGGGL SLPV NA+KISYTPLQINPLSPTFYYI +
Sbjct: 249 RRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITI 308

Query: 312 KSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 371
            SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP A 
Sbjct: 309 NSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAA 368

Query: 372 QLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSG 431
           +L+PGFDLCVNASGESR  SLP+LRFR+GGG VFAPP RNYF+ETEEGVMCLAIR V+SG
Sbjct: 369 ELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESG 428

Query: 432 NGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463
           NGFSVIGNLMQQGFLLEFD+++SR+GF+RRGCGLP
Sbjct: 429 NGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Cp4.1LG01g17730 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 570.9 bits (1470), Expect = 9.6e-163
Identity = 288/456 (63.16%), Postives = 347/456 (76.10%), Query Frame = 0

Query: 10  LTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSSH 69
           L FFL   L L LL      + +  ++YLK PLL  +PF SP+QAL+ DT RL  L    
Sbjct: 4   LIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 63

Query: 70  RHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPP 129
           +  P +KSP++SGA++GSGQYFV+L +G PPQSLLL+ADTGSDLVWVKCS CRNCSHH P
Sbjct: 64  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 123

Query: 130 SSAFFPRHSSSFSPFHCFDPHCRLLPHPP-SHRCNHTHLHSPCPFLYSYADSSLSSGFFS 189
           ++ FFPRHSS+FSP HC+DP CRL+P P  +  CNHT +HS C + Y YAD SL+SG F+
Sbjct: 124 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFA 183

Query: 190 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 249
           ++ T+  T SG   + RL  ++FGCGFRISG SVSG  FNGA GVMGLGRGPISF+SQLG
Sbjct: 184 RETTSLKTSSG--KEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 243

Query: 250 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 309
           RRFGN FSYCLMDYTLSPPPTSYL+IG G   +     SK+ +TPL  NPLSPTFYY+ +
Sbjct: 244 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI-----SKLFFTPLLTNPLSPTFYYVKL 303

Query: 310 KSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 369
           KS+ V+G KL I+P++W ID+ GNGGTVVDSGTTL +LAE AY  V+ A+RRRVKLP A 
Sbjct: 304 KSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 363

Query: 370 QLSPGFDLCVNASGESR-MRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDS 429
            L+PGFDLCVN SG ++  + LP+L+F   GG VF PP RNYF+ETEE + CLAI+ VD 
Sbjct: 364 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 423

Query: 430 GNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 464
             GFSVIGNLMQQGFL EFDRD+SR+GFSRRGC LP
Sbjct: 424 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

BLAST of Cp4.1LG01g17730 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.1e-105
Identity = 216/456 (47.37%), Postives = 262/456 (57.46%), Query Frame = 0

Query: 10  LTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSSH 69
           L FFL   L L LL      + +  ++YLK PLL  +PF SP+QAL+ DT RL  L    
Sbjct: 4   LIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 63

Query: 70  RHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPP 129
           +  P +KSP++SGA++GSGQYFV+L +G PPQSLLL+ADTGSDLVWVKCS CRNCSHH P
Sbjct: 64  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 123

Query: 130 SSAFFPRHSSSFSPFHCFDPHCRLLPHPP-SHRCNHTHLHSPCPFLYSYADSSLSSGFFS 189
           ++ FFPRHSS+FSP HC+DP CRL+P P  +  CNHT +HS C + Y YAD SL+SG F+
Sbjct: 124 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFA 183

Query: 190 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 249
           ++ T+  T SG   + RL  ++FGCGFRISG SVS                         
Sbjct: 184 RETTSLKTSSG--KEARLKSVAFGCGFRISGQSVS------------------------- 243

Query: 250 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 309
                                                                       
Sbjct: 244 ------------------------------------------------------------ 303

Query: 310 KSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 369
                                 GNGGTVVDSGTTL +LAE AY  V+ A+RRRVKLP A 
Sbjct: 304 ----------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 350

Query: 370 QLSPGFDLCVNASGESR-MRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDS 429
            L+PGFDLCVN SG ++  + LP+L+F   GG VF PP RNYF+ETEE + CLAI+ VD 
Sbjct: 364 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 350

Query: 430 GNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 464
             GFSVIGNLMQQGFL EFDRD+SR+GFSRRGC LP
Sbjct: 424 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

BLAST of Cp4.1LG01g17730 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 246.5 bits (628), Expect = 4.2e-65
Identity = 156/408 (38.24%), Postives = 225/408 (55.15%), Query Frame = 0

Query: 59  THRLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKC 118
           T  +SL+ +       L + L SG + GSG+YF+++ +GTPP+   L+ DTGSDL W++C
Sbjct: 129 TSDISLVGAPEVSPGKLIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQC 188

Query: 119 SPCRNCSHHPPSSAFF-PRHSSSFSPFHCFDPHCRLLPHP-PSHRCNHTHLHSPCPFLYS 178
            PC +C H   +  F+ P+ S+SF    C DP C L+  P P  +C     +  CP+ Y 
Sbjct: 189 LPCYDCFHQ--NGMFYDPKTSASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYW 248

Query: 179 YADSSLSSGFFSKDVTTFN--TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVM 238
           Y D S ++G F+ +  T N  T  G  ++ ++ ++ FGCG    G       F+GA G++
Sbjct: 249 YGDRSNTTGDFAVETFTVNLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLL 308

Query: 239 GLGRGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPL 298
           GLGRGP+SFSSQL   +G++FSYCL+D   +   +S L+ G     L  TN +  S+   
Sbjct: 309 GLGRGPLSFSSQLQSLYGHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG 368

Query: 299 QINPLSPTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEV 358
           + N +  TFYYI +KSI V G  L I    W I   G+GGT++DSGTTL+Y AE AYE +
Sbjct: 369 KENSVE-TFYYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEII 428

Query: 359 LKAMRRRVKLPRAL-QLSPGFDLCVNASG-ESRMRSLPQLRFRVGGGGVFAPPARNYFVE 418
                 ++K    + +  P  D C N SG E     LP+L      G V+  PA N F+ 
Sbjct: 429 KNKFAEKMKENYPIFRDFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIW 488

Query: 419 TEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGC 461
             E ++CLAI        FS+IGN  QQ F + +D  +SR+GF+   C
Sbjct: 489 LSEDLVCLAILGTPKST-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of Cp4.1LG01g17730 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-61
Identity = 146/391 (37.34%), Postives = 213/391 (54.48%), Query Frame = 0

Query: 75  LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFF 134
           L + L SG + GSG+YF+++ +G+PP+   L+ DTGSDL W++C PC +C     + AF+
Sbjct: 155 LVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFY 214

Query: 135 -PRHSSSFSPFHCFDPHCRLLPHP-PSHRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVT 194
            P+ S+S+    C D  C L+  P P   C     +  CP+ Y Y DSS ++G F+ +  
Sbjct: 215 DPKASASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETF 274

Query: 195 TFN--TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRR 254
           T N  T  G+     + ++ FGCG    G       F+GA G++GLGRGP+SFSSQL   
Sbjct: 275 TVNLTTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSL 334

Query: 255 FGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKS 314
           +G++FSYCL+D       +S L+ G     L   N +  S+   + N L  TFYY+ +KS
Sbjct: 335 YGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKS 394

Query: 315 ITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQL 374
           I V G  L I    W I   G GGT++DSGTTL+Y AE AYE +   +  + K    +  
Sbjct: 395 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 454

Query: 375 S-PGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGN 434
             P  D C N SG   ++ LP+L      G V+  P  N F+   E ++CLA+      +
Sbjct: 455 DFPILDPCFNVSGIHNVQ-LPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPK-S 514

Query: 435 GFSVIGNLMQQGFLLEFDRDKSRMGFSRRGC 461
            FS+IGN  QQ F + +D  +SR+G++   C
Sbjct: 515 AFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

BLAST of Cp4.1LG01g17730 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 223.0 bits (567), Expect = 4.9e-58
Identity = 165/465 (35.48%), Postives = 238/465 (51.18%), Query Frame = 0

Query: 25  SLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSSHR-------------H 84
           SLTD S +  +  L   L H +  SS S A  +D   L L   S R              
Sbjct: 48  SLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGR 107

Query: 85  SPTLKSP---------LISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCR 144
           + T ++P         +ISG S GSG+YF+ L +GTP  ++ +V DTGSD+VW++CSPC+
Sbjct: 108 NATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCK 167

Query: 145 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSL 204
            C ++   + F P+ S +F+   C    CR L    S  C  T     C +  SY D S 
Sbjct: 168 AC-YNQTDAIFDPKKSKTFATVPCGSRLCRRL--DDSSEC-VTRRSKTCLYQVSYGDGSF 227

Query: 205 SSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPIS 264
           + G FS +  TF+         R++ +  GCG    G       F GA G++GLGRG +S
Sbjct: 228 TEGDFSTETLTFH-------GARVDHVPLGCGHDNEG------LFVGAAGLLGLGRGGLS 287

Query: 265 FSSQLGRRFGNTFSYCLMDYT----LSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINP 324
           F SQ   R+   FSYCL+D T     S PP++ +    G  ++P T+     +TPL  NP
Sbjct: 288 FPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVF---GNAAVPKTSV----FTPLLTNP 347

Query: 325 LSPTFYYIVVKSITVDGVKLP-INPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA 384
              TFYY+ +  I+V G ++P ++ + + +D  GNGG ++DSGT++T L + AY  +  A
Sbjct: 348 KLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDA 407

Query: 385 MR-RRVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETE-E 444
            R    KL RA   S  FD C + SG + ++ +P + F  GGG V + PA NY +    E
Sbjct: 408 FRLGATKLKRAPSYSL-FDTCFDLSGMTTVK-VPTVVFHFGGGEV-SLPASNYLIPVNTE 467

Query: 445 GVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGC 461
           G  C A     +    S+IGN+ QQGF + +D   SR+GF  R C
Sbjct: 468 GRFCFAF--AGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483