Cp4.1LG01g17270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g17270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCarboxypeptidase
LocationCp4.1LG01: 11806460 .. 11809615 (-)
RNA-Seq ExpressionCp4.1LG01g17270
SyntenyCp4.1LG01g17270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTTAAGCTTCTCATTCTCTCAACAATGGCTAAACTATTAGTTCTTCGTCTTTGTTTTGTTCTCATTGCTTCCTCTTTTTTGTTTCACGTTGGAGGTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAGTAAGTCAAATTCATTAATTTTCATTCTATTTTATTTTAGTCATTATTTATTATATATTTATTCTTGGGATTGCGTTGTTCAGAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCGAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTCTGGTTACAAGGCGGACCGGTGAGCAACCGACCTTTTCCAACGATTTATTTATAAATTTTGTGTACTTTGTAAGAAAATGGCTCAAATATTGACAAACAGGGTGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATCGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGTCAACTCCTTTTCCTTTTAATTTTCACCATTTTAACCTTAAATTCTAATATTTTACACTAAATTTATGATTTTAACATTAAAAAAAAATAGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCGAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGGGCCATCCAACCTGGCAATTTGACCCTTAATCTCGGAGGTACTTTTATTTTTTTTTTTTTTAATAAAAAGTTTCAAATATAAATGATGAATTATAACCTTTTTTTTATATGTAAAAAAAAAAAAAAAAATCATATTATAACTTTGATTTGTTTAAAATGATCCCGTTGTAATAATATAGAATCAATAATTTAAATACACGAATCTCGATTTTGTTTGCCAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGGTTCGTATTCTCATTCTCTCATTCTCATCCAAAATTTTTATCAATTTTTTTTTTCGATATTTTCGTTTTTAGAATGATAAACGAAAAAAATACTCCTAAACTACAAAGTTGATTTCAATTATATTCTGATCTAAGAGTTAAAATGAAATATCTCAAAGCTCGAGAGTATAACCGAAACCAACCTCAAATTTCAAGAGTATTCTTTTATTATTTATCCTTAACACAATAATCTTTGTCTAAAATTTTACAGTCTTCTTGGGGTCCTCTTCTCAAAGATCTCTCACGAATAGACGACACGACCCTCCAAAAATCAAATAGGTTAATATTTTTCACTTTTTTTTTTTTTTTAATATAAACAATAATAAAGAAGTGAAATATATTCTACAAATATAAAAATTATATGTAACAAAAGCTGTCTTTTGAAAATCAGTTTAGCGTTGAAGATCAAGCAGCAAATTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTTGAGGGATTAATTTTCTCCAATAGCAACCACGTGGTACGTTTTGTTATTATTATTATTATTATTATTATTATTTTGAAAAAATCCTCCCATACCAATGAAATTTAAATTTAAATTTATAGTAACTTAAATAGGTCTATGGAGTCTTTATTTTTATTATTTTTCTTATTAAGTTATCTTTGATGGATATATTAGACGTATAATTTAAAGTTTAGAACTTTTCTAAATTACCAATTTAGGCTTCATTCATATCTATTTAATTTTTTAAGTTTATAAAAACTATTTTGACCTACAAATTCCTTTTATTTTATCATCTTCAGTTTTAAAAAAATATTATATTTTTTTCCGAGAGAACGAACATTAGTTTTAAAAAGACATAAAACGAAATGATTATCGATATGTTATAACGAGTCGTTTTTGCAGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCAGTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATGTATACAAACCCGGAGCTAAAGGAGAGTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCACCAAATGTCACGTACGTACCAATAAACCCTATTTTCTTTAAATAAAATAATAATCATTAAATGAAGCCAAGGATTATATTAATTATCTAAATTTGGAGATTAAATCCTACCATTTTAGCCTTAAAAACTAAAATATAAATTTTGTATGTATAGATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACCGCAGATTTTATGAAGCCAAGGATCAACGAGGTGTTTTTTTTTTTAACCAAACTATTTTTAATATTTTTATTTGTGGATTATTTTTTGCTTCATAATATAACATTCAATTATGTCCTAATATTTTTATTTTTTCCAAAAGGTTGATGAGTTACTGGTTAAAGGTATTAATGTGACCGTCTACGGCGGTCAAGTAAGTATTTTTTTTCCCTTTTTTCAGATTCATAATTTCAAACAAAAAACAAACAATATTTTGAAGTGATAAAAATGATAATATTCTCTTTGATTTATATAGGTCGATCTTATTTGTTCAACCAAGGGAACAGAGACGTGGATTAATAAGCTCAAGTAATTTGTTTCTAAGTAATTTATGTTATGGACATAAACACAAACACGTCTTCCTAAAAGAAAAAATATCCATGTTTAAATTCTTAATTTTTACTATTCTTTTTCTTTTGGAATTTAGGTGGGAAGGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAATGAGAAGCACACCAAAGGTTTCACTAAGTCACATAAGAACTTGCACTTCTATTGGATTCTTGGCGCTGGCCATTTTGTAAGCTCTCTATATATAAACTTTATTGTATTTATAATTTTTTTTATAGATTTAATACTTTTTAATTATATGATTACAAATGTAGGTTCCCGTGGATCAACCGTGTGTGACATTAAGTATGGTGGATGCAATCACGCAGTCACCAGCTTCT

mRNA sequence

AACTTAAGCTTCTCATTCTCTCAACAATGGCTAAACTATTAGTTCTTCGTCTTTGTTTTGTTCTCATTGCTTCCTCTTTTTTGTTTCACGTTGGAGGTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCGAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTCTGGTTACAAGGCGGACCGGGTGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATCGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCGAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGGGCCATCCAACCTGGCAATTTGACCCTTAATCTCGGAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGATCAAGCAGCAAATTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTTGAGGGATTAATTTTCTCCAATAGCAACCACGTGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCAGTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATGTATACAAACCCGGAGCTAAAGGAGAGTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCACCAAATGTCACATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACCGCAGATTTTATGAAGCCAAGGATCAACGAGGTTGATGAGTTACTGGTTAAAGGTATTAATGTGACCGTCTACGGCGGTCAAGTCGATCTTATTTGTTCAACCAAGGGAACAGAGACGTGGATTAATAAGCTCAAGTGGGAAGGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAATGAGAAGCACACCAAAGGTTTCACTAAGTCACATAAGAACTTGCACTTCTATTGGATTCTTGGCGCTGGCCATTTTGTTCCCGTGGATCAACCGTGTGTGACATTAAGTATGGTGGATGCAATCACGCAGTCACCAGCTTCT

Coding sequence (CDS)

ATGGCTAAACTATTAGTTCTTCGTCTTTGTTTTGTTCTCATTGCTTCCTCTTTTTTGTTTCACGTTGGAGGTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCGAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTCTGGTTACAAGGCGGACCGGGTGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATCGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCGAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGGGCCATCCAACCTGGCAATTTGACCCTTAATCTCGGAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGATCAAGCAGCAAATTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTTGAGGGATTAATTTTCTCCAATAGCAACCACGTGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCAGTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATGTATACAAACCCGGAGCTAAAGGAGAGTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCACCAAATGTCACATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACCGCAGATTTTATGAAGCCAAGGATCAACGAGGTTGATGAGTTACTGGTTAAAGGTATTAATGTGACCGTCTACGGCGGTCAAGTCGATCTTATTTGTTCAACCAAGGGAACAGAGACGTGGATTAATAAGCTCAAGTGGGAAGGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAATGAGAAGCACACCAAAGGTTTCACTAAGTCACATAAGAACTTGCACTTCTATTGGATTCTTGGCGCTGGCCATTTTGTTCCCGTGGATCAACCGTGTGTGACATTAAGTATGGTGGATGCAATCACGCAGTCACCAGCTTCT

Protein sequence

MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGVSLGNSWMSPEDYTIKQQIKNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS
Homology
BLAST of Cp4.1LG01g17270 vs. ExPASy Swiss-Prot
Match: Q67Y83 (Serine carboxypeptidase-like 51 OS=Arabidopsis thaliana OX=3702 GN=SCPL51 PE=2 SV=2)

HSP 1 Score: 477.2 bits (1227), Expect = 1.9e-133
Identity = 242/437 (55.38%), Postives = 300/437 (68.65%), Query Frame = 0

Query: 30  SQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEI 89
           + DG+E WGYV+VRPKAHMFWW Y+SP RV+  SKPWP ILWLQGGPG SG G GNF E+
Sbjct: 29  NSDGSEAWGYVEVRPKAHMFWWHYKSPYRVENPSKPWPIILWLQGGPGASGVGIGNFQEV 88

Query: 90  GPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDVEAATDMTTLLAEV 149
           GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D EAA D+T LL ++
Sbjct: 89  GPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQL 148

Query: 150 SNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGVSLGNSWMSPEDYT 209
            N    L  +PL+I AESYGGK AV L LS++ A+Q G L L+LGGV LG+SW+SPED+ 
Sbjct: 149 FNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFV 208

Query: 210 ---------------------------IKQQIKNRLYENATLSWNDLEGLIFSNSNHVDF 269
                                      IK QIKN  Y  AT +W DLE LI S SN VDF
Sbjct: 209 FSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDLENLISSKSNFVDF 268

Query: 270 YNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNSPYL----SLNVYKPGAKGELEALMN 329
           YNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  +   +G+L+ LMN
Sbjct: 269 YNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDVE-DVEGDLDKLMN 328

Query: 330 GPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQVDLIC 389
           G IK+KL IIP ++ WG  SD VF    A FMKP I +VDELL  G++VT+Y GQ+D+IC
Sbjct: 329 GVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGVDVTIYNGQLDVIC 388

Query: 390 STKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPV 434
           ST GTE W++KL+WEGL+ F   ER  LFC +++ T+GFTKS+KNLHFYWILGAGHFVPV
Sbjct: 389 STSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSYKNLHFYWILGAGHFVPV 448

BLAST of Cp4.1LG01g17270 vs. ExPASy Swiss-Prot
Match: Q920A6 (Retinoid-inducible serine carboxypeptidase OS=Rattus norvegicus OX=10116 GN=Scpep1 PE=2 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.6e-92
Identity = 196/458 (42.79%), Postives = 260/458 (56.77%), Query Frame = 0

Query: 3   KLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVG 62
           ++ ++RL  +L++    F  G ++  + Q+G E W YV VR  A MFWWLY + +     
Sbjct: 6   RICLVRLWLLLLSFLLGFSAGSALNWREQEGKEVWDYVTVREDARMFWWLYYATNPCKNF 65

Query: 63  SKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSY 122
           S+  P ++WLQGGPGGS TGFGNF EIGPL++ LKPRN+TWLQ A LLFVDNPVGTG+SY
Sbjct: 66  SE-LPLVMWLQGGPGGSSTGFGNFEEIGPLDTRLKPRNTTWLQWASLLFVDNPVGTGFSY 125

Query: 123 VESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAI 182
           V +   +AK     A+DM  LL    +     +  P YIF+ESYGGK A  ++L L +AI
Sbjct: 126 VNTTDAYAKDLDTVASDMMVLLKSFFDCHKEFQTVPFYIFSESYGGKMAAGISLELHKAI 185

Query: 183 QPGNLTLNLGGVSLGNSWMSPEDYT-----------------------IKQQIKNRL--- 242
           Q G +  N  GV+LG+SW+SP D                         I +Q+ N +   
Sbjct: 186 QQGTIKCNFSGVALGDSWISPVDSVLSWGPYLYSVSLLDNKGLAEVSDIAEQVLNAVNKG 245

Query: 243 -YENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNS 302
            Y+ AT  W   E +I  N++ V+FYN         L  +T   S+ES    S+E  ++ 
Sbjct: 246 FYKEATQLWGKAEMIIEKNTDGVNFYNI--------LTKSTPDTSMES----SLEFFRSP 305

Query: 303 PYLSLNVYKPGAKGE-LEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINE 362
                  +    +G+ L  LMNGPIK+KL IIP +V+WG QS  VF     DFMKP I+ 
Sbjct: 306 LVRLCQRHVRHLQGDALSQLMNGPIKKKLKIIPDDVSWGAQSSSVFISMEEDFMKPVIDI 365

Query: 363 VDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCG-NEKHTK 422
           VD LL  G+NVTVY GQ+DLI  T G E+W+ KLKW  L  F   +   L+       T 
Sbjct: 366 VDTLLELGVNVTVYNGQLDLIVDTIGQESWVQKLKWPQLSRFNQLKWKALYTNPKSSETS 425

Query: 423 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQ 432
            F KS++NL FYWIL AGH VP DQ  + L M+  +TQ
Sbjct: 426 AFVKSYENLAFYWILKAGHMVPADQGDMALKMMRLVTQ 450

BLAST of Cp4.1LG01g17270 vs. ExPASy Swiss-Prot
Match: Q9HB40 (Retinoid-inducible serine carboxypeptidase OS=Homo sapiens OX=9606 GN=SCPEP1 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.1e-92
Identity = 194/440 (44.09%), Postives = 250/440 (56.82%), Query Frame = 0

Query: 21  HVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSG 80
           + G  I   +++G E W YV VR  A+MFWWLY + +     S+  P ++WLQGGPGGS 
Sbjct: 24  NAGAVIDWPTEEGKEVWDYVTVRKDAYMFWWLYYATNSCKNFSE-LPLVMWLQGGPGGSS 83

Query: 81  TGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDM 140
           TGFGNF EIGPL+S+LKPR +TWLQ A LLFVDNPVGTG+SYV   G +AK     A+DM
Sbjct: 84  TGFGNFEEIGPLDSDLKPRKTTWLQAASLLFVDNPVGTGFSYVNGSGAYAKDLAMVASDM 143

Query: 141 TTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGVSLGNSW 200
             LL    +     +  P YIF+ESYGGK A  + L L +AIQ G +  N  GV+LG+SW
Sbjct: 144 MVLLKTFFSCHKEFQTVPFYIFSESYGGKMAAGIGLELYKAIQRGTIKCNFAGVALGDSW 203

Query: 201 MSPEDYT-----------------------IKQQIKNR----LYENATLSWNDLEGLIFS 260
           +SP D                         + +Q+ N     LY  AT  W   E +I  
Sbjct: 204 ISPVDSVLSWGPYLYSMSLLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQ 263

Query: 261 NSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVE-VRKNSPYLSLNVYKPGAKGELE 320
           N++ V+FYN         L  +T T+++ES    S+E  + +   L     +   +  L 
Sbjct: 264 NTDGVNFYNI--------LTKSTPTSTMES----SLEFTQSHLVCLCQRHVRHLQRDALS 323

Query: 321 ALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQV 380
            LMNGPI++KL IIP + +WGGQ+  VF     DFMKP I+ VDELL  GINVTVY GQ+
Sbjct: 324 QLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQL 383

Query: 381 DLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEK-HTKGFTKSHKNLHFYWILGAG 432
           DLI  T G E W+ KLKW  L  F   +   L+   +   T  F KS+KNL FYWIL AG
Sbjct: 384 DLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAG 443

BLAST of Cp4.1LG01g17270 vs. ExPASy Swiss-Prot
Match: Q920A5 (Retinoid-inducible serine carboxypeptidase OS=Mus musculus OX=10090 GN=Scpep1 PE=1 SV=2)

HSP 1 Score: 339.0 bits (868), Expect = 8.1e-92
Identity = 187/458 (40.83%), Postives = 255/458 (55.68%), Query Frame = 0

Query: 3   KLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVG 62
           ++ ++RL  +L++    F  G +I  +  +G E W YV VR  AHMFWWLY + +     
Sbjct: 6   RICLVRLWLLLLSFLLGFSAGSAIDWREPEGKEVWDYVTVRKDAHMFWWLYYATNPCKNF 65

Query: 63  SKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSY 122
           S+  P ++WLQGGPGGS TGFGNF EIGPL++ LKPRN+TWLQ A LLFVDNPVGTG+SY
Sbjct: 66  SE-LPLVMWLQGGPGGSSTGFGNFEEIGPLDTQLKPRNTTWLQWASLLFVDNPVGTGFSY 125

Query: 123 VESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAI 182
           V +   +AK     A+DM  LL    +     +  P YIF+ESYGGK A  +++ L +A+
Sbjct: 126 VNTTDAYAKDLDTVASDMMVLLKSFFDCHKEFQTVPFYIFSESYGGKMAAGISVELYKAV 185

Query: 183 QPGNLTLNLGGVSLGNSWMSPEDYTIK---------------------------QQIKNR 242
           Q G +  N  GV+LG+SW+SP D  +                              +   
Sbjct: 186 QQGTIKCNFSGVALGDSWISPVDSVLSWGPYLYSMSLLDNQGLAEVSDIAEQVLDAVNKG 245

Query: 243 LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNS 302
            Y+ AT  W   E +I  N++ V+FYN +            T +S E     S+E  ++ 
Sbjct: 246 FYKEATQLWGKAEMIIEKNTDGVNFYNIL------------TKSSPEKAMESSLEFLRSP 305

Query: 303 PYLSLNVYKPGAKGE-LEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINE 362
                  +    +G+ L  LMNGPIK+KL IIP +++WG Q+  VF     DFMKP I+ 
Sbjct: 306 LVRLCQRHVRHLQGDALSQLMNGPIKKKLKIIPEDISWGAQASYVFLSMEGDFMKPAIDV 365

Query: 363 VDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCG-NEKHTK 422
           VD+LL  G+NVTVY GQ+DLI  T G E+W+ KLKW  L  F   +   L+       T 
Sbjct: 366 VDKLLAAGVNVTVYNGQLDLIVDTIGQESWVQKLKWPQLSKFNQLKWKALYTDPKSSETA 425

Query: 423 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQ 432
            F KS++NL FYWIL AGH VP DQ  + L M+  +T+
Sbjct: 426 AFVKSYENLAFYWILKAGHMVPSDQGEMALKMMKLVTK 450

BLAST of Cp4.1LG01g17270 vs. ExPASy Swiss-Prot
Match: C9WMM5 (Venom serine carboxypeptidase OS=Apis mellifera OX=7460 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.4e-35
Identity = 114/426 (26.76%), Postives = 197/426 (46.24%), Query Frame = 0

Query: 26  ITHKSQDGTEEW-GYVQVRPK--AHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTG 85
           I HK  +    + G++ V  K  ++MF+W +  P+  D   K  P +LWLQGGPG +   
Sbjct: 61  IQHKEVEAISSYAGFLTVNKKYNSNMFFWFF--PALHD--PKTAPVVLWLQGGPGATSM- 120

Query: 86  FGNFLEIGPL----NSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAAT 145
           +G FLE GP     N  LK R  +W +  +LL++DNPVGTG+S+ E    +A  +     
Sbjct: 121 YGLFLENGPFIVTKNKTLKMREYSWNKCHNLLYIDNPVGTGFSFTEDERGYATNETHVGR 180

Query: 146 DMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGVSLGN 205
           D+ T L +       L+    Y+  ESYGGK+   ++ ++        + +NL G+++GN
Sbjct: 181 DVHTALVQFFELFPELQTNDFYVTGESYGGKYVPAVSHAIKDYNIKAKIKINLKGLAIGN 240

Query: 206 SWMSP------EDYTIKQQIKNRLYENATLSWNDLEGLIFSNSNHV---DFYNFMLD--- 265
               P       DY  +  + +    N    + +    +      +   D ++ +LD   
Sbjct: 241 GLTDPVNQLDYGDYLYQLGLLDANGRNLFQKYEEQGKNLIKQEKWLEAFDLFDELLDGDI 300

Query: 266 FEMDSLAATTTTNSVESNRIDSVEVRKNSPYLSLNVYKPGAKGELEALMNGPIKQKLTII 325
            +  SL    T      N +   +   +S Y+            +E L    +++ + + 
Sbjct: 301 TQQPSLYKNLTGFDYYFNYLHEKDPSNDSDYM------------VEWLQRADVRKAIHV- 360

Query: 326 PPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQVDLICSTKGTETWIN 385
             N T+  +S  V ++  AD M+     + + L +   V +Y GQ+D+I +   TE ++ 
Sbjct: 361 -GNRTFIPESKKVEKYMKADVMQSLAVLIAD-LTQHYRVLIYNGQLDIIVAYPLTENYLQ 420

Query: 386 KLKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMV 433
           KLKW G + + + +R   F GNE    G++K+  +L    +  AGH VP+DQP   L ++
Sbjct: 421 KLKWPGAEKYKTAQRKVWFVGNE--LAGYSKTVDSLTEVLVRNAGHMVPLDQPKWALDLI 464

BLAST of Cp4.1LG01g17270 vs. NCBI nr
Match: XP_023515401.1 (serine carboxypeptidase-like 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 435/462 (94.16%), Postives = 435/462 (94.16%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQPGNLTLNLGGVSLGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDTTLQKSNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK
Sbjct: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN
Sbjct: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 462

BLAST of Cp4.1LG01g17270 vs. NCBI nr
Match: XP_022975190.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 822 bits (2122), Expect = 5.07e-299
Identity = 407/462 (88.10%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL LCFVLIASS LFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT  WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. NCBI nr
Match: XP_022985845.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 819 bits (2116), Expect = 4.17e-298
Identity = 405/462 (87.66%), Postives = 417/462 (90.26%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL LCFVLIASS LFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMD+LAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDTLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQS+LVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSNLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT+ WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTQVWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. NCBI nr
Match: XP_022975497.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 818 bits (2112), Expect = 1.69e-297
Identity = 405/462 (87.66%), Postives = 415/462 (89.83%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL LCFVLIASS LF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT  WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. NCBI nr
Match: XP_022975494.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 813 bits (2101), Expect = 8.04e-296
Identity = 403/462 (87.23%), Postives = 413/462 (89.39%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL LCFVLIASS LF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVD LL KG+NVTVY GQVDLICSTKGT  WINK +WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDVLLAKGVNVTVYSGQVDLICSTKGTHAWINKFEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. ExPASy TrEMBL
Match: A0A6J1IG20 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474281 PE=3 SV=1)

HSP 1 Score: 822 bits (2122), Expect = 2.46e-299
Identity = 407/462 (88.10%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL LCFVLIASS LFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT  WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. ExPASy TrEMBL
Match: A0A6J1JCE4 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111483771 PE=3 SV=1)

HSP 1 Score: 819 bits (2116), Expect = 2.02e-298
Identity = 405/462 (87.66%), Postives = 417/462 (90.26%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL LCFVLIASS LFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMD+LAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDTLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQS+LVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSNLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT+ WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTQVWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. ExPASy TrEMBL
Match: A0A6J1ID81 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474900 PE=3 SV=1)

HSP 1 Score: 818 bits (2112), Expect = 8.21e-298
Identity = 405/462 (87.66%), Postives = 415/462 (89.83%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL LCFVLIASS LF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGT  WINKL+WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. ExPASy TrEMBL
Match: A0A6J1IKN9 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474883 PE=3 SV=1)

HSP 1 Score: 813 bits (2101), Expect = 3.89e-296
Identity = 403/462 (87.23%), Postives = 413/462 (89.39%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL LCFVLIASS LF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQIK 240
           AIQ GNLTLNLGGV+LGNSWMSPEDYT                           IKQQIK
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDS+EVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360
           NSPYLSLNVYKPGA G+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420
           EVD LL KG+NVTVY GQVDLICSTKGT  WINK +WEGLKAFISKERTTLFCGNEKHTK
Sbjct: 361 EVDVLLAKGVNVTVYSGQVDLICSTKGTHAWINKFEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           GFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSP S
Sbjct: 421 GFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPTS 462

BLAST of Cp4.1LG01g17270 vs. ExPASy TrEMBL
Match: A0A6J1JE07 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111484161 PE=3 SV=1)

HSP 1 Score: 743 bits (1919), Expect = 2.30e-268
Identity = 378/467 (80.94%), Postives = 394/467 (84.37%), Query Frame = 0

Query: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M K  VLRLCF LIAS  LFHVG SI  K QDG EEWGYVQVRPKAHMFWWLYRSP RV 
Sbjct: 1   MDKRFVLRLCFTLIASFTLFHVGTSI--KFQDGNEEWGYVQVRPKAHMFWWLYRSPLRVY 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
            GSKPWPTILWLQGGPGGSGTGFGNFLEIGPLN NLKPRNSTWLQKADLLFVDNPVGTGY
Sbjct: 61  AGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNLNLKPRNSTWLQKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVS-NNTVGLRNTPLYIFAESYGGKFAVTLALSLL 180
           SYVESGGEFAKGDVEAA DMTTLLAEVS NNTV LRN  LYIFAESYGGKFA+TLALSLL
Sbjct: 121 SYVESGGEFAKGDVEAAIDMTTLLAEVSKNNTVCLRNAHLYIFAESYGGKFAITLALSLL 180

Query: 181 RAIQPGNLTLNLGGVSLGNSWMSPEDYT---------------------------IKQQI 240
           +AIQ GNL LNLGGV+LGNSWMSPEDYT                           IKQQI
Sbjct: 181 QAIQAGNLALNLGGVALGNSWMSPEDYTFSWGPLLKDLSRIDDVTLQASNRLAWKIKQQI 240

Query: 241 KNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATT----TTNSVESNRIDS 300
           KN+LYENAT+SW+DLE LI + SNHVDFYNFMLDFEMD L +TT    TT+SVE NRIDS
Sbjct: 241 KNKLYENATVSWSDLEELIITKSNHVDFYNFMLDFEMDPLTSTTITTTTTSSVEPNRIDS 300

Query: 301 VEVRKNSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFM 360
            EV+KNS YLS ++YKPG+ G LEALMNGPIKQKL IIPPNV WGGQSDLVFQFFT DFM
Sbjct: 301 FEVKKNSRYLSSHIYKPGSGGGLEALMNGPIKQKLAIIPPNVKWGGQSDLVFQFFTRDFM 360

Query: 361 KPRINEVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGN 420
           K RINEVDELL KGINVT+Y GQVDLICSTKGTE WINKLKWEGLKAFI+KERT LFCGN
Sbjct: 361 KSRINEVDELLAKGINVTIYNGQVDLICSTKGTEAWINKLKWEGLKAFINKERTPLFCGN 420

Query: 421 EKHTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSPAS 435
           EK TKGFTKSHKNLHFYWILGAGHFVPVDQPCVTL+MVDAITQSPAS
Sbjct: 421 EKQTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLNMVDAITQSPAS 465

BLAST of Cp4.1LG01g17270 vs. TAIR 10
Match: AT2G27920.1 (serine carboxypeptidase-like 51 )

HSP 1 Score: 477.2 bits (1227), Expect = 1.4e-134
Identity = 242/437 (55.38%), Postives = 300/437 (68.65%), Query Frame = 0

Query: 30  SQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEI 89
           + DG+E WGYV+VRPKAHMFWW Y+SP RV+  SKPWP ILWLQGGPG SG G GNF E+
Sbjct: 29  NSDGSEAWGYVEVRPKAHMFWWHYKSPYRVENPSKPWPIILWLQGGPGASGVGIGNFQEV 88

Query: 90  GPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDVEAATDMTTLLAEV 149
           GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D EAA D+T LL ++
Sbjct: 89  GPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQL 148

Query: 150 SNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGVSLGNSWMSPEDYT 209
            N    L  +PL+I AESYGGK AV L LS++ A+Q G L L+LGGV LG+SW+SPED+ 
Sbjct: 149 FNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFV 208

Query: 210 ---------------------------IKQQIKNRLYENATLSWNDLEGLIFSNSNHVDF 269
                                      IK QIKN  Y  AT +W DLE LI S SN VDF
Sbjct: 209 FSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDLENLISSKSNFVDF 268

Query: 270 YNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNSPYL----SLNVYKPGAKGELEALMN 329
           YNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  +   +G+L+ LMN
Sbjct: 269 YNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDVE-DVEGDLDKLMN 328

Query: 330 GPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQVDLIC 389
           G IK+KL IIP ++ WG  SD VF    A FMKP I +VDELL  G++VT+Y GQ+D+IC
Sbjct: 329 GVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGVDVTIYNGQLDVIC 388

Query: 390 STKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPV 434
           ST GTE W++KL+WEGL+ F   ER  LFC +++ T+GFTKS+KNLHFYWILGAGHFVPV
Sbjct: 389 STSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSYKNLHFYWILGAGHFVPV 448

BLAST of Cp4.1LG01g17270 vs. TAIR 10
Match: AT2G27920.2 (serine carboxypeptidase-like 51 )

HSP 1 Score: 392.9 bits (1008), Expect = 3.4e-109
Identity = 207/390 (53.08%), Postives = 260/390 (66.67%), Query Frame = 0

Query: 77  GGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDV 136
           G SG G GNF E+GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D 
Sbjct: 4   GASGVGIGNFQEVGPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDE 63

Query: 137 EAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLTLNLGGV 196
           EAA D+T LL ++ N    L  +PL+I AESYGGK AV L LS++ A+Q G L L+LGGV
Sbjct: 64  EAAQDLTKLLQQLFNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGV 123

Query: 197 SLGNSWMSPEDYT---------------------------IKQQIKNRLYENATLSWNDL 256
            LG+SW+SPED+                            IK QIKN  Y  AT +W DL
Sbjct: 124 ILGDSWISPEDFVFSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDL 183

Query: 257 EGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNSPYL----SLNVY 316
           E LI S SN VDFYNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  
Sbjct: 184 ENLISSKSNFVDFYNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDV 243

Query: 317 KPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGI 376
           +   +G+L+ LMNG IK+KL IIP ++ WG  SD VF    A FMKP I +VDELL  G+
Sbjct: 244 E-DVEGDLDKLMNGVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGV 303

Query: 377 NVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLH 434
           +VT+Y GQ+D+ICST GTE W++KL+WEGL+ F   ER  LFC +++ T+GFTKS+KNLH
Sbjct: 304 DVTIYNGQLDVICSTSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSYKNLH 363

BLAST of Cp4.1LG01g17270 vs. TAIR 10
Match: AT2G27920.3 (serine carboxypeptidase-like 51 )

HSP 1 Score: 330.5 bits (846), Expect = 2.1e-90
Identity = 178/351 (50.71%), Postives = 226/351 (64.39%), Query Frame = 0

Query: 116 VGTGYSYVESGGE--FAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVT 175
           VG GYS+VE   +  + K D EAA D+T LL ++ N    L  +PL+I AESYGGK AV 
Sbjct: 48  VGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQLFNKNQTLNQSPLFIVAESYGGKIAVK 107

Query: 176 LALSLLRAIQPGNLTLNLGGVSLGNSWMSPEDYT-------------------------- 235
           L LS++ A+Q G L L+LGGV LG+SW+SPED+                           
Sbjct: 108 LGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFVFSWGPLLKHVSRLDDNGLDSSNSLAE 167

Query: 236 -IKQQIKNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRI 295
            IK QIKN  Y  AT +W DLE LI S SN VDFYNF+LD  MD ++ TT+    +  +I
Sbjct: 168 KIKTQIKNGEYVGATQTWMDLENLISSKSNFVDFYNFLLDTGMDPVSLTTSLKIKKEEKI 227

Query: 296 DSVEVRKNSPYL----SLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQF 355
                +K S YL    SL+  +   +G+L+ LMNG IK+KL IIP ++ WG  SD VF  
Sbjct: 228 -----KKYSRYLNDMRSLSDVE-DVEGDLDKLMNGVIKKKLKIIPNDLIWGNNSDDVFTA 287

Query: 356 FTADFMKPRINEVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERT 415
             A FMKP I +VDELL  G++VT+Y GQ+D+ICST GTE W++KL+WEGL+ F   ER 
Sbjct: 288 MEAAFMKPVIEDVDELLATGVDVTIYNGQLDVICSTSGTEAWVHKLRWEGLEEFKKMERE 347

Query: 416 TLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVDAITQSP 434
            LFC +++ T+GFTKS+KNLHFYWILGAGHFVPVD+PCV L MV  IT+SP
Sbjct: 348 PLFCESDRATRGFTKSYKNLHFYWILGAGHFVPVDEPCVALKMVGEITKSP 392

BLAST of Cp4.1LG01g17270 vs. TAIR 10
Match: AT3G45010.1 (serine carboxypeptidase-like 48 )

HSP 1 Score: 125.9 bits (315), Expect = 7.7e-29
Identity = 109/428 (25.47%), Postives = 184/428 (42.99%), Query Frame = 0

Query: 31  QDGTEEWGYVQV--RPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLE 90
           QD     GY ++     A MF++ + S +     +K  P ++WL GGPG S +    F E
Sbjct: 97  QDFGHHAGYYKLPNSKAARMFYFFFESRT-----NKADPVVIWLTGGPGCS-SELALFYE 156

Query: 91  IGPL----NSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDMTTLL 150
            GP     NS+L      W + ++L++VD PVGTG+SY     +    +   + D+   L
Sbjct: 157 NGPFTVSNNSSLSWNEFGWDKASNLIYVDQPVGTGFSYTSDQSDLRHDEDGVSNDLYDFL 216

Query: 151 AEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQPGNLT-LNLGGVSLGNSWMSP 210
                          YI  ESY G +   LA  + R  +    T +NL G ++GN   +P
Sbjct: 217 QAFFKEHPQFVKNDFYITGESYAGHYIPALASRVHRGNKNKEGTHINLKGFAIGNGLTNP 276

Query: 211 EDYTIKQQIKNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDF------------EMDS 270
           E       I+   Y +  L  N     + + S+H +   +                E D+
Sbjct: 277 E-------IQYGAYADYALDMN-----LITQSDHDNLNRYYATCQQSIKECSADGGEGDA 336

Query: 271 LAATTTTNSVESNRIDSVEVRKNSPYLSLNVYKPGAK----GELEALMNGPIKQKLTIIP 330
            A++ T  +    +I  +++  N  Y  +     G+       +E  +N    +K   + 
Sbjct: 337 CASSYTVCNNIFQKI--MDIAGNVNYYDVRKQCEGSLCYDFSNMENFLNQKSVRKALGV- 396

Query: 331 PNVTWGGQSDLVFQFFTADFMKPRINEVDELLVKGINVTVYGGQVDLICSTKGTETWINK 390
            ++ +   S  V++    D+M+     +  LL  GI + VY G+ DLIC+  G   W+++
Sbjct: 397 GDIEFVSCSTAVYEAMQMDWMRNLEVGIPALLQDGIKLLVYAGEYDLICNWLGNSKWVHE 456

Query: 391 LKWEGLKAFISKERTTLFCGNEKHTKGFTKSHKNLHFYWILGAGHFVPVDQPCVTLSMVD 436
           ++W G K F++         N++   G  K++ +L F  +  AGH VP+DQP   L M+ 
Sbjct: 457 MEWSGQKEFVAAATVPFHVDNKE--AGLMKNYGSLTFLKVHDAGHMVPMDQPKAALQMLQ 501

BLAST of Cp4.1LG01g17270 vs. TAIR 10
Match: AT5G22980.1 (serine carboxypeptidase-like 47 )

HSP 1 Score: 122.9 bits (307), Expect = 6.5e-28
Identity = 109/451 (24.17%), Postives = 193/451 (42.79%), Query Frame = 0

Query: 3   KLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQV--RPKAHMFWWLYRSPSRVD 62
           +L+  ++ F+  A S    VG S+    Q+     GY  +     A MF++ + S ++  
Sbjct: 70  RLIERQIDFLATAGSKNASVGPSV----QEFGHYAGYYSLPHSKSAKMFYFFFESRNKTT 129

Query: 63  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPL----NSNLKPRNSTWLQKADLLFVDNPV 122
                 P ++WL GGPG S +    F E GP     + +L   +  W + +++++VD PV
Sbjct: 130 D-----PVVIWLTGGPGCS-SSVAMFYENGPFKISKDLSLYWNDFGWDKVSNIIYVDQPV 189

Query: 123 GTGYSYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLAL 182
           GTG+SY     +    +   + D+   L               +I  ESY G +   LA 
Sbjct: 190 GTGFSYTSDESDIRNDEDGVSNDLYDFLQAFFKEHPKFVKNDFFITGESYAGHYIPALA- 249

Query: 183 SLLRAIQPGN-----LTLNLGGVSLGNSWMSPE-------DYTIKQQIKNRLYENATLSW 242
                +  GN     + +NL G ++GN   +PE       DY ++ ++         +S 
Sbjct: 250 ---SRVHSGNKKKEGIPINLKGFAIGNGLTNPEIQYGAYGDYALQMKL---------ISE 309

Query: 243 NDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRKNSPYLSLNVYK 302
           +D E L     ++V+  N      +       +   V ++  + +  +K+     LN Y 
Sbjct: 310 SDHESL---KQDYVECQNITKKCSLGGGLVCDSAVEVCTSIFNKIVAKKS----GLNYYD 369

Query: 303 PGAK---------GELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRINEV 362
              K           +E  +N    +K   +  ++ +   S  V+     D+M+    ++
Sbjct: 370 IRKKCVGSLCYDFSRMEIFLNKENVRKALGV-GDIKFVSCSSTVYDAMIEDWMQNLEVKI 429

Query: 363 DELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTKGF 422
             L+  GIN+ VY G+ DLIC+  G   W++++ W G K F S +  +     ++   G 
Sbjct: 430 PSLVNDGINLLVYAGEYDLICNWLGNSRWVDQMNWSGQKGFGSAKNVSFLVDGKE--AGL 487

Query: 423 TKSHKNLHFYWILGAGHFVPVDQPCVTLSMV 427
            K+H  L F  +  AGH VP+DQP  +L M+
Sbjct: 490 LKNHGPLTFLKVYNAGHMVPMDQPKASLQML 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67Y831.9e-13355.38Serine carboxypeptidase-like 51 OS=Arabidopsis thaliana OX=3702 GN=SCPL51 PE=2 S... [more]
Q920A61.6e-9242.79Retinoid-inducible serine carboxypeptidase OS=Rattus norvegicus OX=10116 GN=Scpe... [more]
Q9HB402.1e-9244.09Retinoid-inducible serine carboxypeptidase OS=Homo sapiens OX=9606 GN=SCPEP1 PE=... [more]
Q920A58.1e-9240.83Retinoid-inducible serine carboxypeptidase OS=Mus musculus OX=10090 GN=Scpep1 PE... [more]
C9WMM55.4e-3526.76Venom serine carboxypeptidase OS=Apis mellifera OX=7460 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023515401.10.094.16serine carboxypeptidase-like 51 [Cucurbita pepo subsp. pepo][more]
XP_022975190.15.07e-29988.10serine carboxypeptidase-like 51 [Cucurbita maxima][more]
XP_022985845.14.17e-29887.66serine carboxypeptidase-like 51 [Cucurbita maxima][more]
XP_022975497.11.69e-29787.66serine carboxypeptidase-like 51 [Cucurbita maxima][more]
XP_022975494.18.04e-29687.23serine carboxypeptidase-like 51 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1IG202.46e-29988.10Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474281 PE=3 SV=1[more]
A0A6J1JCE42.02e-29887.66Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111483771 PE=3 SV=1[more]
A0A6J1ID818.21e-29887.66Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474900 PE=3 SV=1[more]
A0A6J1IKN93.89e-29687.23Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474883 PE=3 SV=1[more]
A0A6J1JE072.30e-26880.94Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111484161 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G27920.11.4e-13455.38serine carboxypeptidase-like 51 [more]
AT2G27920.23.4e-10953.08serine carboxypeptidase-like 51 [more]
AT2G27920.32.1e-9050.71serine carboxypeptidase-like 51 [more]
AT3G45010.17.7e-2925.47serine carboxypeptidase-like 48 [more]
AT5G22980.16.5e-2824.17serine carboxypeptidase-like 47 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001563Peptidase S10, serine carboxypeptidasePRINTSPR00724CRBOXYPTASECcoord: 112..122
score: 58.22
coord: 99..111
score: 24.85
coord: 147..172
score: 23.89
coord: 402..415
score: 46.43
IPR001563Peptidase S10, serine carboxypeptidasePFAMPF00450Peptidase_S10coord: 31..429
e-value: 1.8E-70
score: 238.5
IPR001563Peptidase S10, serine carboxypeptidasePANTHERPTHR11802SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASEcoord: 19..206
IPR001563Peptidase S10, serine carboxypeptidasePANTHERPTHR11802SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASEcoord: 208..433
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 19..434
e-value: 1.2E-84
score: 287.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 28..431
NoneNo IPR availablePANTHERPTHR11802:SF345CARBOXYPEPTIDASEcoord: 208..433
NoneNo IPR availablePANTHERPTHR11802:SF345CARBOXYPEPTIDASEcoord: 19..206
IPR018202Serine carboxypeptidase, serine active sitePROSITEPS00131CARBOXYPEPT_SER_SERcoord: 161..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g17270.1Cp4.1LG01g17270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004185 serine-type carboxypeptidase activity