Cp4.1LG01g16710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g16710
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Thiamine-phosphate pyrophosphorylase
Location	Cp4.1LG01: 10460313 .. 10469592 (+)
RNA-Seq Expression	Cp4.1LG01g16710
Synteny	Cp4.1LG01g16710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCCCATTACCAGTCACTGTACTCCGTCCCGCCGCCTCCTGCCCGCCGCCTCCTCCTCCCCCTCCCCCTCACATTCTCTCGCCCTCCCAAGGACTCAAGAGTCACATTTCTTTGTTTAATTCCCTCAGAAACACAGTGGTTCAGCCCCTAACCCGCGCCGTTCTTGAGATGGTGCTGCTGCCTCTCACTTTTCAGATTCCTAAGGTCTGATCTTTTTTTGTTTCTTTTTATTCTTTTTCGTTGGGTTATGCGCTTCATCCAAGGAGAAGAAAACTAATAATGTATCCCTCGCAAATTAGACAGAAAACACCGATTGAACCAACAAGTACAGAGGAACATAAAACAAACCAATTCTTGTATGAATCAGTAAATTTATAGGAGAAGTAATGTTTAAGAATGAACAATGCTTAAAAAGACCCTGTAGTTTTGTTGGCTATGAAAAACTTGTTGGAATTGAAAAGCTGTATTTGAAAACATTGAAACTATAGCAAACTATGGACAAACAAAATAGAAAACTTAACTTTTGATTTTAGTTTGATTGAATGACGTTGAGTCTTCTTGAAAATAGAAGAAACTATCCACGGAGAGAAGAAATTATTATCTATTTTTTAAAAAAATTTCATATGGAGTAGAGTTGGAAACAGAATTTTGAAGTATTAAATCCATACAAATCAGACCTATATAGAAGTATATTTAGGGGTTCCAACACGGGTTAGCTTTATCAATTATGGAGGTATATTTTATGATTTATGGGTTTTACTTTCTGTGCCTTGATTTGTCCTTTTTAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCTATGAGATGAGGATTCCACATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACAGCACAGAACACCGTGGGGGTTCAGGTCATTATCACACACACATAATTATTATCCATATTTATTTGTTGGTGTATTTGTTTTGGCTTGAAAGGACAATGGGGAGGACTGGTGAATAAATAAATAATGTTCCTTGCCTGCCTGCTATGGTATAATGGGGTGTATAATGTTATTGTGAGTTTAGGATGATTTTTGAAAACAAGGTGCTTCTGAGTGAAAGCTTATCCTTTGTTAGCACTTTATTAAGTGCTATCCTGGGTATGTTTAGGAATGCTTTAGGCATGGTGAAAAACACCTTTTTCTTATGCTCAAAAGCATGTATCATAAATATCATGTTTGGGAGCAACAACAGATGCTTTTAAAAAAGTTAATAGTACTTATAATACTTGGTAAAAAGCACTTTTAGAGTGCTTTTGTCTAACTGGGAAGTACTTTATAAACACTTATTGATAGTGTGAAAGCAAGCATAAAGTAATCTTTCGTAAAGCAGTTCTTTTCAAAATGCTTACAGTACTTCATCCTTATTCTATAAGCACTCCTAAATACATCCGTAGTCCTTTTAATAAGTTTTCAAGTATCACTTCTAAGTATTTAACTCAAATTATGAAAACTTTGAAAATACTTTTGACAAGCCAGAAACACTTTTTACCTTTGCGAAAATCATCCTAAACTCACTCTTAGAATATACAATGCCCTTGAAGTTAGTTTTTGCTTTGTTCTCAATGAAATTAGCTGAAATGTTTTGTCCTAAATTATACTGCTTGTAACACAAATTTTATTTAAGACGAAGATGTACTAACTTTTTCAGGATGTAAACATTATGCCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAGTATAATTAAATTTTAAGTTAGGATTGCCTTGCAAGTTTTTTAGGAGCTCATATGGACAAGACTGTGGACAGTTCATGTATAGGATATTGAACAGGCTAGCTGTTATTGCTTTTTCAGTGCACGTACTAATATATACTTTATGTAAGCTGGAAAACACAGAAAGACTACTAATGATTACATTAATTCAGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGTTATACGTCAGCGCCTGAAGGAGTTTCCTGTTCAAGGTATAAAGTATTTCAGTAAGTTATTGGTGCATTTCTTTGATTTAATTATCGTTTTTTAAAGATTTTAATATGAATAAAGGCAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGATGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGTAAGTTGATTATCTGTTTATAGGGAGTATCATTAGTCCTTCAAACTAGTTCTTATGATAATTTCCTGCGCTTCCTGTTTGCATTTTTTAAATGTCCTCAGAATTAGTATATTGTTTTTTGGTTCAAAAGGACTCTGAAGCTTTTGGTATGGACTTCAAGCTTTTAGCGTTAAGGCTTACATCGAACGAACCTTTATAAGAACCTACTGAGGCTTGAAATTCTTATGCCTCATATAATAATTAGAAAAATAATACTATTTTATTGCCTATTCTCTCAAAATGTGAATGTTCACCTGTGTAAGTAATGCTTATAGCACCACAATTTATTCATGCTTTTCGCTGATGTCCATACATCCAAGTAAGGACTACATGAAACTCCCAACAATTTAATACTGTTTTTAATGGTATTGCTACTGTTTTTACTTTCACATGTTAGATTGAGTGATTTTGCAAAACCCCTTCGTTTGCAAGGGGACATCTTGTCCGTTTTTCCATATACTGTTTTGGAAGCTTTTTTTTAATAATGAAATTTCTTGCATGTTTCTTATAAAAAGAAGAGAGAGAGAGCGTGAAAAGGAGAAAAATCTTCTTGGTAGTAGCTTCTTCTTAAAGTTGCTGGTTTTTCTTGTTGAGAGAGGCTTTTAGTTTTGACTTGGCAAAACTTGTTAGTCTTTGTTTGGCTGCAATTACTCCCACAAATTTGCCTCACATTGGCCCCAAGGTGTGTCCTTGTTGGTAAGAAATTACAATATTGGAACCCCTCCCAAATCTTTAATTTAAATAGTGGGTTCTAGGGATCGAGAGAATTAATTTCCTTGCCCATCTACTAGAGCTGGGGCTGCGGGTTTTGCTGGGGATTAGAGTTGGTGGGGCCTGGATTCCCCAATTTACAGCCATAATTTGCTTAATTGAATGAAAATAATGCTTTTTCTTTAATCAAAATTCATGTCTGATCTTCATATGTTAGTTTTTCATAATTCTATGATACGTCTTTTGCTTTCAGGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGGTATGCCCCTTAATACAATTTCTGACATGCGTCATGCTGCAACGCTAATCCATCAGATGGGATCCAAGTAAGTTTTTAATCCATCACATAGCTTGCGTTCTTTTGTAATTCCATTTTATCAATGAAATGAAAATCAATGTTTCTCATGAAAAAAGAAGAAGAAATTTTATGCTACCTCTAGTCACAAGAGACTGACTTTGACACTCAATTTTCCTCATTGCCGCCTGCCTCCTAGCAGTAATGTTAATACCATTCTACTTAATTCAAAGAAGTTTTTTTAAGTCTAAATTATAAAAATACCCCTGAATTTTACTCTTTGTTTCAAAAGTTCAGAGGTGTCTTTTATAATTTAACCATTTTTAAAACTTTAATTCTATGGTTATTCGGATTACATTAGCGATCGCTAGTCATTCTCCCTCCTTTGCCACCCAATCTTCACCTTCTTATAGACCCATCGAATCTTCGCCCCCTTGCCTTCTCAATCCCTTGACCAACCCTCCTCAACAATCGCTAGCCATCCTGACCCCAATCCCTTCTTCCTTTCCCTCCTCATCAGCCAATTAACCTACCTCACTGCATTTACCAATTGGCCTCGAGATGACTACAAATTCCATGGAGTAATCAGGGACAAAAAAAAGGCTCAACGAAAATCGTGATTGGCGACGAAGAAGACAGGAGGTGTATCTGATATTGGGAGAAAACCCTTCATATTTTCCTTGACCCTTATGTTAACCTCCATCATATTCATCCGAGAGAAAGATTTAGTGGCTGTATCGATGTATCCTCCATGGGCATTCCAAATTTTCTTGAAGGATTCAAAAGATCATTTCTTGAAGGATATTTATCAATTGGAAAATTTCTGATTTTCATCCATCCCCACTGGGTAGGCACTGGAGGATCTTTAAGAAAGGACTCCATACTCAAATTTTCAAATCTTACCCTATAAGTTCCCACCTTATACCACCTATATTTCCCAAAGTAGTCTCTCGTTCTTTGCCTTCCTCCACGAGGAGTGCCCTATCAGGTTGAATGGGTCTAATAAACCCAAAATCATATACTTATTGTTAAAGAGCTCATAAAATCACATGCCCGTCATTATGAAAGTGCACAAAAGATAAAAGGATTGATCTCTGCTTGTTTCAGTGATTTTTAACCCTTTTCCACCCTCAATCAGTGATTGGTGCGCACCGTCGAGTTAGGCAGCAGGAGAGCATGGTAGGGAGGGGTGGGATTGGGTTGGGTATGTGAGAAGAGAGAGAAGGGAACATTTTTTTTCTCTTAAATCAAACAGAGTGAAAGCCAATTGGAGAGCTTTGTTGTAATTCACCAATAGTTGCTTGGAGTTTTTACTCCCGTGTTTCATCAACTAGTGAAATTGTTTCTTCTTCTTCTTCTTCTTCAAAATAATATAACAATAAATTAATAAAAATCTTTCTAGTTTTCTAACAACAAAATGTTGTAGGTGCACATGGGTGTCCATGAAATTAGTGAGGTGTCCACCAGTTGGCCTAAATGCTCAATCCATGGATATCAACGAAAGGAAGAAAAACAATGGGGTTTATAAAATTTTTGGAAGGAAGAAAAAAAAAAAGGGAGCTGCAAAAAGAGTGACGGAGGGAGGTTGAGAGAAGCAAATGTTTAGTTTAGGCTTGGGTTTACTCAAATAACAGCATCCAAATTTCTCTTAGAATTATTTGTTTATTTATTTTTCTAATTTCTTATTGATTTAAAACCCTAGCTAATTGGATCTAAGAACCCTTATCAACCCTTAGATATTCTAACCCATAAATCACAAATTCTAAAACCCCCAAATGAAGATGAGTTCATCCCAAATGATTGTAAGTATCGAGAATTCAATTTGAAGAATAAGACCTTTAGATCTAAAATATCCCAGAACTCTAAAATGACAAGGAATACCCCAAGGAAAGTTAGATCCGGATTATTTTATTGATCAAATATCTCAAGTATCACAAGGCAAGAAAACTTGTTTGAGATTTGAATCACTTCACCAGCAGAATCAATCATGTCAAGCTTAAATGATTCTAAGCATACAACCTAAACTATATAGAATTGCAAATCAACTTAGCCCTTGGCTAAGAGAAAGCATGAATGCTATTTTTACTATATTTTCTAAGTCTATCTTACAAAGACAACATGCATGGCTTTATATAGCCTCAAAATGAAACCCTTGACCTTTCATGAGGCATTTCAAAAGTTGTAACCTTCATACTTCATGGCTATAATTGGCCACTAAGGAAATGTATCCTAGAAACATCATAAATTTAGATTATCATCTACCAAAGAATTTGTAACCATCCAAGAATAAATGAAGTTCCACCTGACGTAGTTTCATTGTTGCACAATTGAAGTTTCATTGTTGCACAATTGAAGTTTCATTGTTGCACAATTGAAGCTTCATTGTTGCACAATTGAAGCTTGATTCTTCTTTAATGTGACATTAATTGCAACTTGAGCTTATCTCGTGGTAATTTGAACCACATTTTTTCACATCTTTCTGACATGATTGTTAGTTCTGCATGTTACAACTATTAAGATAATTTTGAGTTCAATTTTCTACCCAATTAAATTCAACCTTGCACATTTTAGACGCCCCCTCCGCTTCATACTACCATTTTTATCAATTTACTCCTGACTGATGCTGAAGAGCGTTATGTTTGATTTTAGAAATGTACTTGTCAAAGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGTAAGTACAGTACAGATTGCATAATTCTGACAATTTGATATATATATTTTTTTGTTATGAAGGAAGCCCATCCACTGAATCCATATTTTTTTCATAGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGGTAATAATCTACGGACACTTATTTTAAACCCACCAATAAGAAGATTAAAGTCTCAACGGTTCATAATACAACTTCCTATTGAATAGTTAAATAATTTTTTAAAAATAAACATCTTCAATTTGTTATAGGATAATAGTTGTTGAAAATAACGCTATATTCAATATGTATGCTCACTGACCTTGACAGATGATCAAGACGGGATGTTAAGAGTTACTTTAATGCTTTTTTTTTTCTGGCTTTTCATTGAAATGATGAAAAATAAAAAAATGTTCTAGGGATACAAACTCCCAAAGGGAGTGAAAAAGGGAAAAAGGAAAAAAAATACAAACAGTACATACAACCAAATAGAGATTAGTCAGGAAAATAAATAAATTTTTAAGGAACCAAGAACTTTCTCTGAACACTTGGAGAGCTTGCCAATGAATAATGAAACTGTGAATAAAACCTCTATCAAACACTGAAATTCGATAGATGCTTCAAAACTTCAAAAAGAAAACCAAGAATCGACCACAACCAACAACTCTTGAGCCAATTTTACTCGTGACACCTTCATCAACTCTTGAGCTTTGCAAGAATTACTAACCATTGTTAAAAGCTTTGTAAGAACGATCACCGACAAAGAAATAAAAAAATATCCTCATGCAAGTCAATTCAAATATTTCATGCAAGTCGATTCAAATATCCCAGTGAAGACAAAAAAAAACTTTGTAAAGCAAAACCATGACGTGGCTACCCTCTCGAGCTAATGGAGAGTCTTCTTGTCACAGTCCTGAAATAATAAGCCAAAAGTTTAAGAAAAAGAATCTACAAGAACATCAACCTCCCTACTGAAATCCATGGATGAATGAGTGATTCCACGCTGTTCAAACGTACCTGGGAGTCAACCTCAAACACGTCATTACATGGTGGTGAGTTCTTTTTCGAGTGAATGACTCCTCTCGTTAAACCTTGAGTGAAGTCAAAATGGTTGTTGAGGGGATAAGTGAAGGGGGAGGGTGGTAAGGCGACATTCTAATTGAATAAGAATTTAAAGAAGTTTCCTGGAAGCATGAATGGTAAGAAGAAAAAAGCACATCTTTTTTGTGAGAATAATGCAAATGATAGGCTGGAATATATAAACATTCTTCCCCTCCTTTTTAACACTTGTGGGCTTGGAAATCTCAATCCGTAATGGAGGCCTCTAGGATTCTCAATAAATGAGGGATATATCTTTCACAGTCCTATTTGATAATCAAGCTTTTAAAAGTTGTACTTATTTTTTCACCTTTCTTTACAGCATCTTTCCTAATGAAACATTTAAATTCACGACCAAATTCTATTTACAAACCCAAGGTTTTCAAAACTACTTTTTTATGCACTAATCAAAATATGGTTGCCACTCAATCCAATTTGGAGCTTCAAATTCTTTGGTTATTTGTTTTTTTCTTTTTCTTTTTTTTTGGATGAAAGAGACGTATCATTTCACTCCATATATATAATTTGAGTAATTCAAAACGCAATAGGCTTGGTTGTCGTTACCAGACTTGATGATTGTCCACAACTAAGGTTTTATTTATCTGGATCTTTACAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATCATTCAGATAAGGTTTGTCTACTGTTTTCCATTAATCAATTGTTGTTAGTTTGTTTGCTTAAAAATGTTTTCAGAAGTTCATACATATTCAAGTCATTACTTCAAATGTATTGAATTCCTTGTTGCATTTTAATACCTCTTAGCACCTTCTCAGTTGTTCAAAGGTTGAATGGCTTTAGAAACTTACCCATAAAGTGTAGGCTAAATATGCTCCCTCTATAGGCTAGCAATAGTGAGGTAGTAATCTTGGGTTAATAGAACCCGACTGTAAGATTTGAATCTCTTAACGTGTTGCATTATTGTTGACCGAAGTTATAAACTTGGTATAATTAAAACTTGTATTCAATTGTTAATTTGCTTCCAATGGTCGCATGATCTTATTTTTATGAACAGGGAAAAGGATGCTAAAACTCACGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGTGTTCCATTACTGATCAACGATCGTATTGACGTCGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATCGGGTGTGGTGGAGTTTATCCCACAAACACAAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACCTTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGAGTGAGTGAGGGACCATAGTGCAACGACACAGTTTCTTGATTTTGATGAAATCAAATGTATGAATAATTGTAATGCTTTGATTTTAGTAGAAAGGTTTTTGAAATAAATGTAATTGTCTCAATGTGATTATAATGTAACAAAAATTTAATTTCGTGATAATAAGCATGGTCATTATATATTCTTTAAAA

mRNA sequence

CGCCCATTACCAGTCACTGTACTCCGTCCCGCCGCCTCCTGCCCGCCGCCTCCTCCTCCCCCTCCCCCTCACATTCTCTCGCCCTCCCAAGGACTCAAGAGTCACATTTCTTTGTTTAATTCCCTCAGAAACACAGTGGTTCAGCCCCTAACCCGCGCCGTTCTTGAGATGGTGCTGCTGCCTCTCACTTTTCAGATTCCTAAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCTATGAGATGAGGATTCCACATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACAGCACAGAACACCGTGGGGGTTCAGGATGTAAACATTATGCCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGTTATACGTCAGCGCCTGAAGGAGTTTCCTGTTCAAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGATGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATCATTCAGATAAGGGAAAAGGATGCTAAAACTCACGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGTGTTCCATTACTGATCAACGATCGTATTGACGTCGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATCGGGTGTGGTGGAGTTTATCCCACAAACACAAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACCTTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGAGTGAGTGAGGGACCATAGTGCAACGACACAGTTTCTTGATTTTGATGAAATCAAATGTATGAATAATTGTAATGCTTTGATTTTAGTAGAAAGGTTTTTGAAATAAATGTAATTGTCTCAATGTGATTATAATGTAACAAAAATTTAATTTCGTGATAATAAGCATGGTCATTATATATTCTTTAAAA

Coding sequence (CDS)

ATGGTGCTGCTGCCTCTCACTTTTCAGATTCCTAAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCTATGAGATGAGGATTCCACATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACAGCACAGAACACCGTGGGGGTTCAGGATGTAAACATTATGCCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGTTATACGTCAGCGCCTGAAGGAGTTTCCTGTTCAAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGATGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATCATTCAGATAAGGGAAAAGGATGCTAAAACTCACGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGTGTTCCATTACTGATCAACGATCGTATTGACGTCGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATCGGGTGTGGTGGAGTTTATCCCACAAACACAAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACCTTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGA

Protein sequence

MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK

Homology

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot
Match: Q5M731 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TH1 PE=1 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 1.7e-189
Identity = 341/491 (69.45%), Postives = 397/491 (80.86%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITAVTAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD + DVVKTGMLPST I++V+ Q L +FPV+ALVVDPVMVSTSG VLA  +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQDELLPMADLVTPNLKEASALLG----------------------------GGDLP 219
           +S+ ++ LLP+AD++TPN+KEASALL                             GGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFI 279
           DS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM SAVK++K+F+
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 ERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDR 339
           + AL YSKDI IG+G QGPFDH   LK   QSS R   FN  DLFLYAVTDS MNK+W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACG 399
           SI DA+KAA+EGGATIIQ+REK+A+T +FLE AK+CI+IC +HGV LLINDRID+ALAC 
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVH+GQSD+PV   RSLLGPDKIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 503
           N T+GLDGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVSALFD+ CVL +
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot
Match: O48881 (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus OX=3708 GN=BTH1 PE=1 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 2.0e-182
Identity = 328/491 (66.80%), Postives = 391/491 (79.63%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVS 99
           ++  VL+VAGSDSGAGAGIQAD+K CAARGVYC++V TAV A+NT  VQ V+++P   VS
Sbjct: 31  KVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDSVS 90

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD +VDVVKTGMLPS  I++V+ Q L E+PV+ALVVDPVMVSTSG VLA  +I
Sbjct: 91  EQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGSSI 150

Query: 160 ISVLQDELLPMADLVTPNLKEASALLG----------------------------GGDLP 219
           +S+ ++ LLP+AD++TPN+KEASALLG                            GGDLP
Sbjct: 151 LSIFRERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSLHQMGPRFVLVKGGDLP 210

Query: 220 DSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFI 279
           DS D+VD++FDG + HEL S RI TRNTHGTGC+LASCIAAELAKGS+M SAVK++K+F+
Sbjct: 211 DSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAKRFV 270

Query: 280 ERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDR 339
           + AL+YSKDI IG+G QGPFDH   LK  +  SYRQ +F   DLFLYAVTDS MNK+W+R
Sbjct: 271 DSALNYSKDIVIGSGMQGPFDHFLSLK--DPQSYRQSTFKPDDLFLYAVTDSRMNKKWNR 330

Query: 340 SITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACG 399
           SI DAVKAA+EGGATIIQ+REK+A+T +FLE AKSC++IC ++GV LLINDR D+A+A  
Sbjct: 331 SIVDAVKAAIEGGATIIQLREKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALD 390

Query: 400 ADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVH+GQSD+PV   RSLLGPDKIIGVSCKT EQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 391 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKAN 450

Query: 460 NLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 503
           N T+GLDGL+ VC ASKLPVVAIGGI  SNA +VM IG PNLKGVAVVSALFD++CVL +
Sbjct: 451 NRTIGLDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQ 510

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot
Match: Q2QWK9 (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 630.2 bits (1624), Expect = 2.1e-179
Identity = 319/500 (63.80%), Postives = 388/500 (77.60%), Query Frame = 0


Query: 32  ASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           ++S   EM  PHVL+VAGSDSG GAGIQAD+K CAA G YCS+V+TAVTAQNT GVQ ++
Sbjct: 47  SASAAREMPWPHVLTVAGSDSGGGAGIQADIKACAALGAYCSSVVTAVTAQNTAGVQGIH 106

Query: 92  IMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSG 151
           ++PE F+ EQL SVLSDM VDVVKTGMLPS G+++V+ + LK+FPV+ALVVDPVMVSTSG
Sbjct: 107 VVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVRVLCESLKKFPVKALVVDPVMVSTSG 166

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLG------------------------- 211
           D L+  + +SV +DEL  MAD+VTPN+KEAS LLG                         
Sbjct: 167 DTLSESSTLSVYRDELFAMADIVTPNVKEASRLLGGVSLRTVSDMRNAAESIYKFGPKHV 226

Query: 212 ---GGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSA 271
              GGD+ +S DA D+FFDGK+  EL + RI T NTHGTGC+LASCIA+ELAKG++M  A
Sbjct: 227 LVKGGDMLESSDATDVFFDGKEFIELHAHRIKTHNTHGTGCTLASCIASELAKGATMLHA 286

Query: 272 VKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDS 331
           V+++K F+E AL +SKD+ +GNGPQGPFDHL +LK    +   Q SF    LFLYAVTDS
Sbjct: 287 VQVAKNFVESALHHSKDLVVGNGPQGPFDHLFKLKCPPYNVGSQPSFKPDQLFLYAVTDS 346

Query: 332 GMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICHTHGVPLLINDR 391
           GMNK+W RSI +AV+AA+EGGATI+Q+REKD++T +FLEAAK+C+EIC + GVPLLINDR
Sbjct: 347 GMNKKWGRSIKEAVQAAIEGGATIVQLREKDSETREFLEAAKACMEICKSSGVPLLINDR 406

Query: 392 IDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGV 451
           +D+ALAC ADGVH+GQ D+  H  R LLGP KIIGVSCKTP QA+QAW DGADYIGCGGV
Sbjct: 407 VDIALACNADGVHVGQLDMSAHEVRELLGPGKIIGVSCKTPAQAQQAWNDGADYIGCGGV 466

Query: 452 YPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALF 504
           +PT+TKANN T+G DGLK VCLASKLPVVAIGGIN SNA +VME+G+PNLKGVAVVSALF
Sbjct: 467 FPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGINASNAGSVMELGLPNLKGVAVVSALF 526

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot
Match: P56904 (Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=thiD PE=3 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 1.5e-44
Identity = 118/260 (45.38%), Postives = 152/260 (58.46%), Query Frame = 0


Query: 45  LSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKS 104
           LS+AGSDSG GAGIQADLKT +A GVY ++VITA+TAQNT GV  V  +    VS Q+ +
Sbjct: 6   LSIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQNTRGVTAVEDVSAEIVSAQMDA 65

Query: 105 VLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQ 164
           V SD+ V  VK GM+     I  I   L+ F  +A VVDPVMV+TSGD L  P  ++ L 
Sbjct: 66  VFSDLDVKAVKIGMVSRRETIAAIADGLRRFGKRA-VVDPVMVATSGDALLRPDAVAALI 125

Query: 165 DELLPMADLVTPNLKEASALLG----------------------------GGDLPDSLDA 224
           +ELLP+A +VTPNL EA+ + G                            GG L    +A
Sbjct: 126 EELLPLALVVTPNLAEAALMTGRAIAGDEAEMARQAEAIMRTGAHAVLVKGGHLKGQ-EA 185

Query: 225 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 277
            D+FFDG  L  L + RI TRN HGTGC+L++ IAA LAKG  +  AV  +K ++  A+S
Sbjct: 186 TDLFFDGDTLVRLPAGRIETRNDHGTGCTLSAAIAAGLAKGVPLIEAVSAAKAYLHAAIS 245

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot
Match: P61422 (Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=thiDE PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 7.4e-44
Identity = 113/251 (45.02%), Postives = 149/251 (59.36%), Query Frame = 0


Query: 44  VLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLK 103
           VL+VAGSDSG GAGIQADLKT    G Y S+V+TA+TAQNT GV  ++ +P  FV++QL 
Sbjct: 228 VLTVAGSDSGGGAGIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLD 287

Query: 104 SVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVL 163
           +V SD+ VDVVKTGML S   I  I  +L E+  + +VVDPVMV+  G  L     +SVL
Sbjct: 288 AVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAVSVL 347

Query: 164 QDELLPMADLVTPNLKEASALLG---------------------------GGDLPDSLDA 223
           ++ L P+A LVTPN+ EA  L G                           GG L    D+
Sbjct: 348 KERLFPLAYLVTPNIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGHLLAG-DS 407

Query: 224 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 268
           VDI FDG   H   S RI ++NTHGTGC+ AS IA  LA+G  +  A+  +K++I  A+ 
Sbjct: 408 VDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIR 467

[Close]

BLAST of Cp4.1LG01g16710 vs. NCBI nr
Match: XP_023550218.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 509/537 (94.79%), Postives = 509/537 (94.79%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. NCBI nr
Match: XP_022957337.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 505/537 (94.04%), Postives = 507/537 (94.41%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDR+EMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNI+PEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVI QRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIHQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. NCBI nr
Match: XP_022997729.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 504/537 (93.85%), Postives = 506/537 (94.23%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVI QRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPM DLVTPNLKE
Sbjct: 121 STGIIQVICQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMVDLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERAL+YSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALNYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLK+VCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKKVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. NCBI nr
Match: KAG7032412.1 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 958 bits (2477), Expect = 0.0
Identity = 503/537 (93.67%), Postives = 505/537 (94.04%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDR+EMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDS MNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSSMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSC+EIC T GVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCLEICRTRGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. NCBI nr
Match: KAG6601651.1 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 503/537 (93.67%), Postives = 505/537 (94.04%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDR+EMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDS MNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSSMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSC+EIC T GVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCLEICRTCGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL
Match: A0A6J1GYV1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 505/537 (94.04%), Postives = 507/537 (94.41%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDR+EMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNI+PEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVI QRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIHQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL
Match: A0A6J1KCE1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599 PE=3 SV=1)

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 504/537 (93.85%), Postives = 506/537 (94.23%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGIIQVI QRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPM DLVTPNLKE
Sbjct: 121 STGIIQVICQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMVDLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERAL+YSKDISIGNGPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALNYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLK+VCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKKVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 537

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL
Match: A0A6J1GZX4 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 925 bits (2390), Expect = 0.0
Identity = 485/517 (93.81%), Postives = 487/517 (94.20%), Query Frame = 0


Query: 21  MAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 80
           MAMKKPEETVVASSDR+EMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT
Sbjct: 1   MAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 60

Query: 81  AQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQAL 140
           AQNTVGVQDVNI+PEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVI QRLKEFPVQAL
Sbjct: 61  AQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIHQRLKEFPVQAL 120

Query: 141 VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGG------------- 200
           VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGG             
Sbjct: 121 VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGGMPLNTISDMRHAA 180

Query: 201 ---------------GDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA 260
                          GDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA 240

Query: 261 ELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ 320
           ELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ
Sbjct: 241 ELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ 300

Query: 321 SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICH 380
           SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKT DFLEAAKSCIEICH
Sbjct: 301 SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICH 360

Query: 381 THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL 440
           THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL
Sbjct: 361 THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 500
           DGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 480

Query: 501 LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 517

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL
Match: A0A6J1K5X2 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599 PE=3 SV=1)

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 484/517 (93.62%), Postives = 486/517 (94.00%), Query Frame = 0


Query: 21  MAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 80
           MAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT
Sbjct: 1   MAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 60

Query: 81  AQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQAL 140
           AQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVI QRLKEFPVQAL
Sbjct: 61  AQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVICQRLKEFPVQAL 120

Query: 141 VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGG------------- 200
           VVDPVMVSTSGDVLACPTIISVLQDELLPM DLVTPNLKEASALLGG             
Sbjct: 121 VVDPVMVSTSGDVLACPTIISVLQDELLPMVDLVTPNLKEASALLGGMPLNTISDMRHAA 180

Query: 201 ---------------GDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA 260
                          GDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA 240

Query: 261 ELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ 320
           ELAKGSSMFSAVKISKQFIERAL+YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ
Sbjct: 241 ELAKGSSMFSAVKISKQFIERALNYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ 300

Query: 321 SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICH 380
           SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKT DFLEAAKSCIEICH
Sbjct: 301 SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICH 360

Query: 381 THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL 440
           THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL
Sbjct: 361 THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 500
           DGADYIGCGGVYPTNTKANNLTVGLDGLK+VCLASKLPVVAIGGINHSNAAAVMEIGVPN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGLDGLKKVCLASKLPVVAIGGINHSNAAAVMEIGVPN 480

Query: 501 LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 509
           LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK
Sbjct: 481 LKGVAVVSALFDRQCVLEETLKLHATLVEATALSPAK 517

[Close]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL
Match: A0A1S3CCI6 (Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 869 bits (2246), Expect = 6.95e-316
Identity = 451/531 (84.93%), Postives = 475/531 (89.45%), Query Frame = 0


Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MV LPL  QIPKFNQVSRFCMAMKK EE VVASSDRYE RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITA+TAQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180
           STGI+QV+ Q LKEFPV+ALVVDPVMVSTSGDVLA PTIISVLQ+ELLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180

Query: 181 ASALLGG----------------------------GDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGG                            GDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300
           RI +RNTHGTGCSLASCI+AELAKGSSMFSAVK SKQFIERAL YSKDI+IG+GPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360
           HLCRLKSREQSSY QG FN +DLFLYAVTDSGMN+RWDRSITDAVKAAVEGGATI+QIRE
Sbjct: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIRE 360

Query: 361 KDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420
           KDAKT DFLE AKSCI+ICH HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R LLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480
           P+K+IGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVG+DGLKRVCLASKLPVV
Sbjct: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEAT 503
           AIGGINH+NAAAVM IG+PNL+GVAVVSALFDRQCVLE   KLHATLVEAT
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEAT 531

[Close]

BLAST of Cp4.1LG01g16710 vs. TAIR 10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative )

HSP 1 Score: 663.7 bits (1711), Expect = 1.2e-190
Identity = 341/491 (69.45%), Postives = 397/491 (80.86%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITAVTAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQVIRQRLKEFPVQALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD + DVVKTGMLPST I++V+ Q L +FPV+ALVVDPVMVSTSG VLA  +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQDELLPMADLVTPNLKEASALLG----------------------------GGDLP 219
           +S+ ++ LLP+AD++TPN+KEASALL                             GGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFI 279
           DS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM SAVK++K+F+
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 ERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDR 339
           + AL YSKDI IG+G QGPFDH   LK   QSS R   FN  DLFLYAVTDS MNK+W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAAVEGGATIIQIREKDAKTHDFLEAAKSCIEICHTHGVPLLINDRIDVALACG 399
           SI DA+KAA+EGGATIIQ+REK+A+T +FLE AK+CI+IC +HGV LLINDRID+ALAC 
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVH+GQSD+PV   RSLLGPDKIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 503
           N T+GLDGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVSALFD+ CVL +
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g16710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5M731	1.7e-189	69.45	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
O48881	2.0e-182	66.80	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
Q2QWK9	2.1e-179	63.80	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
P56904	1.5e-44	45.38	Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (st...	[more]
P61422	7.4e-44	45.02	Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (st...	[more]

BLAST of Cp4.1LG01g16710 vs. NCBI nr


Match Name	Identity	Description
XP_023550218.1	94.79	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbi...	[more]
XP_022957337.1	94.04	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbi...	[more]
XP_022997729.1	93.85	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbi...	[more]
KAG7032412.1	93.67	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic, partial [Cucurbita...	[more]
KAG6601651.1	93.67	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic, partial [Cucurbita...	[more]

BLAST of Cp4.1LG01g16710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYV1	0.0	94.04	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1KCE1	0.0	93.85	Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599...	[more]
A0A6J1GZX4	0.0	93.81	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1K5X2	0.0	93.62	Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599...	[more]
A0A1S3CCI6	6.95e-316	84.93	Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=...	[more]

BLAST of Cp4.1LG01g16710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22940.1	1.2e-190	69.45	thiamin biosynthesis protein, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR034291	Thiamine phosphate synthase	TIGRFAM	TIGR00693	TIGR00693	coord: 297..486 e-value: 5.2E-62 score: 206.6
IPR034291	Thiamine phosphate synthase	HAMAP	MF_00097	TMP_synthase	coord: 294..501 score: 26.885986
IPR013749	Pyridoxamine kinase/Phosphomethylpyrimidine kinase	PFAM	PF08543	Phos_pyr_kin	coord: 51..186 e-value: 2.7E-51 score: 174.3
IPR022998	Thiamine phosphate synthase/TenI	PFAM	PF02581	TMP-TENI	coord: 297..482 e-value: 8.0E-64 score: 214.3
IPR022998	Thiamine phosphate synthase/TenI	CDD	cd00564	TMP_TenI	coord: 297..497 e-value: 6.55894E-84 score: 256.291
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 192..283 e-value: 7.3E-19 score: 70.1
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 22..191 e-value: 6.9E-60 score: 204.6
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 39..270
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 284..502 e-value: 3.3E-73 score: 247.2
IPR045029	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	PANTHER	PTHR20858	PHOSPHOMETHYLPYRIMIDINE KINASE	coord: 38..187 coord: 184..498
IPR004399	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase domain	CDD	cd01169	HMPP_kinase	coord: 43..258 e-value: 1.88835E-100 score: 300.189
IPR036206	Thiamin phosphate synthase superfamily	SUPERFAMILY	51391	Thiamin phosphate synthase	coord: 293..495

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g16710.1	Cp4.1LG01g16710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity
molecular_function	GO:0003824	catalytic activity