Cp4.1LG01g15840 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g15840
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionvinorine synthase-like
LocationCp4.1LG01: 9731524 .. 9733345 (+)
RNA-Seq ExpressionCp4.1LG01g15840
SyntenyCp4.1LG01g15840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTGTCATCTCCCCCCTTTGACTTTGACTCAGTTCCATGCACTCGCTGTCCATAAATTGGGATCCAAGGCAGAGCTTCATCTTCCACCGCTTCCCCTGTTTTTCTCTTCTCAATTCGGAGTTTCAGTCGATCGGAGAAATAGCCATGGCCGTCGATATCGAAGTTGTTTCAAAAGAAACGGTCAAACCCTCTTCCCCAACGCCGGACAACCACCGCCGTTACGGTTTATCCTTCCTCGACCAAGTCACCGTCGATGTCTACAACCCAATGATCTATTTCTACTCCGCTGGCTCTGCCACCGCCGCCGAAATCTCCGACCATTTAAAAAAATCCCTCTCCACCGTGTTGTCCCATTACTACCCACTCGCTGGCAGAGTCAACTACGACGAGTTGTTTATCGACTGTAATGATGCTGGCGTCCCCTTCATCGAAACCAGAGTCAACTGCCGACTCTCCGATGTAATGAACACGGCGTTTCCTACTGAACTAAACAAACTCCTTCCGTTTGAATTGGATGCTCTTGATGATGTTTCAATGGGGGTTCAATTGAATGTGTTTGAATGCGGCGGTGTTGCTGTCGGGATTTGTATTTCTCATAAGATCAGCGATGCTCTGTCGTTTTTTATTGTCGTTAATGGGTGGGCGGCGGTTTCCCGGGGGGAAAAAGAGGCGCTGGAAACTCATTTATCAGCATCGGAGCTGTTTCCGCCGAGTAAAACACCGATTTACAACACGAGGACAAGTATTTTCAGACAGAGAGTGGCGAAACGGTACCAAATTGATTCAGCGAGTATCGAAACCATTCGAGCTCAATATGCAGAGTACTTCGCCATGGAAAACCAGAGGCGACCTTCAAGAGTTGAGGCTTTGTCCGCCTTCCTCTACGGCCGTTTTATCGCCGCCATTAAAGGCGTAAGCTTTTAAAGTCCCTTTCAAATCCCAATCGTTAAACGAAAAGGGTCTAATTATATGCTCTGTTTTCTTATTATTACAGGCTTCTAACAGCAATCCGGCGAACGGAAGCTCGGAATCGGAGAAGAAGATATTCTTAGTCTGCCACGCCGTGAACATCCGATCAAGACTGGACCCACCAGTTCCGGATTACGCATTCGGAAACTATTACAGAGCCACATTCGCAGTTCCGTCGGAGACGATTTTAAACGACGATTATTGCTACGATTTAGTGAAACTAGCGAGAGAAGAAATCGCCAAAATCGACAAATCGTATTTGGAAAGGCTTCAAGAAAGCAGTAAGTTCTTGGAGGCGATGAAGAAGGCGGCGGCGCAATTCTCCACCGGCGAGCTGATTTCCTGCTCGTTCACTAGCCTGTGCCGGATGCCGATTTACGACGCTGACTTCGGGTGGGGAAAACCGGCGTGGATCAGCTCGCCGGCGTTAATGTTCAAGAACCTGTTTGTGTTGATCGATAAGAAAGACGGCGATGGAGTTGACGTTTATGTTCATCTCAAGCCGGAGAACATGGAGATTCTTGAAGCCGATGAGAAGTTCCTCAAGTATGCCAAATTGCCTTCTAATTAATAATTTCAATGAAATTATTATTATTATTATTTATTACAATAAATGTGATGTAAAAGAATTTGAAACTCGAACCTGAAGGGTAAGAGAAGAATGTTCATTTTAATTGTGAGAATTTCCATTAATTTAATGAATTTTATTTCTTTTATTAATATTTAGAAGATAAGCGGATTATATAATAACAAGTATTTTTAAATATTTGTAAATATTTTTCTGTTTATAAATATTTGTATTAAAAATTAATATTTTAATAATGTGGATAACATTAAAGCATAGGTTGA

mRNA sequence

ATGACTTCCATGCACTCGCTGTCCATAAATTGGGATCCAAGGCAGAGCTTCATCTTCCACCGCTTCCCCTGTTTTTCTCTTCTCAATTCGGAGTTTCAGTCGATCGGAGAAATAGCCATGGCCGTCGATATCGAAGTTGTTTCAAAAGAAACGGTCAAACCCTCTTCCCCAACGCCGGACAACCACCGCCGTTACGGTTTATCCTTCCTCGACCAAGTCACCGTCGATGTCTACAACCCAATGATCTATTTCTACTCCGCTGGCTCTGCCACCGCCGCCGAAATCTCCGACCATTTAAAAAAATCCCTCTCCACCGTGTTGTCCCATTACTACCCACTCGCTGGCAGAGTCAACTACGACGAGTTGTTTATCGACTGTAATGATGCTGGCGTCCCCTTCATCGAAACCAGAGTCAACTGCCGACTCTCCGATGTAATGAACACGGCGTTTCCTACTGAACTAAACAAACTCCTTCCGTTTGAATTGGATGCTCTTGATGATGTTTCAATGGGGGTTCAATTGAATGTGTTTGAATGCGGCGGTGTTGCTGTCGGGATTTGTATTTCTCATAAGATCAGCGATGCTCTGTCGTTTTTTATTGTCGTTAATGGGTGGGCGGCGGTTTCCCGGGGGGAAAAAGAGGCGCTGGAAACTCATTTATCAGCATCGGAGCTGTTTCCGCCGAGTAAAACACCGATTTACAACACGAGGACAAGTATTTTCAGACAGAGAGTGGCGAAACGGTACCAAATTGATTCAGCGAGTATCGAAACCATTCGAGCTCAATATGCAGAGTACTTCGCCATGGAAAACCAGAGGCGACCTTCAAGAGTTGAGGCTTTGTCCGCCTTCCTCTACGGCCGTTTTATCGCCGCCATTAAAGGCGCTTCTAACAGCAATCCGGCGAACGGAAGCTCGGAATCGGAGAAGAAGATATTCTTAGTCTGCCACGCCGTGAACATCCGATCAAGACTGGACCCACCAGTTCCGGATTACGCATTCGGAAACTATTACAGAGCCACATTCGCAGTTCCGTCGGAGACGATTTTAAACGACGATTATTGCTACGATTTAGTGAAACTAGCGAGAGAAGAAATCGCCAAAATCGACAAATCGTATTTGGAAAGGCTTCAAGAAAGCAGTAAGTTCTTGGAGGCGATGAAGAAGGCGGCGGCGCAATTCTCCACCGGCGAGCTGATTTCCTGCTCGTTCACTAGCCTGTGCCGGATGCCGATTTACGACGCTGACTTCGGGTGGGGAAAACCGGCGTGGATCAGCTCGCCGGCGTTAATGTTCAAGAACCTGTTTGTGTTGATCGATAAGAAAGACGGCGATGGAGTTGACGTTTATGTTCATCTCAAGCCGGAGAACATGGAGATTCTTGAAGCCGATGAGAACATAGGTTGA

Coding sequence (CDS)

ATGACTTCCATGCACTCGCTGTCCATAAATTGGGATCCAAGGCAGAGCTTCATCTTCCACCGCTTCCCCTGTTTTTCTCTTCTCAATTCGGAGTTTCAGTCGATCGGAGAAATAGCCATGGCCGTCGATATCGAAGTTGTTTCAAAAGAAACGGTCAAACCCTCTTCCCCAACGCCGGACAACCACCGCCGTTACGGTTTATCCTTCCTCGACCAAGTCACCGTCGATGTCTACAACCCAATGATCTATTTCTACTCCGCTGGCTCTGCCACCGCCGCCGAAATCTCCGACCATTTAAAAAAATCCCTCTCCACCGTGTTGTCCCATTACTACCCACTCGCTGGCAGAGTCAACTACGACGAGTTGTTTATCGACTGTAATGATGCTGGCGTCCCCTTCATCGAAACCAGAGTCAACTGCCGACTCTCCGATGTAATGAACACGGCGTTTCCTACTGAACTAAACAAACTCCTTCCGTTTGAATTGGATGCTCTTGATGATGTTTCAATGGGGGTTCAATTGAATGTGTTTGAATGCGGCGGTGTTGCTGTCGGGATTTGTATTTCTCATAAGATCAGCGATGCTCTGTCGTTTTTTATTGTCGTTAATGGGTGGGCGGCGGTTTCCCGGGGGGAAAAAGAGGCGCTGGAAACTCATTTATCAGCATCGGAGCTGTTTCCGCCGAGTAAAACACCGATTTACAACACGAGGACAAGTATTTTCAGACAGAGAGTGGCGAAACGGTACCAAATTGATTCAGCGAGTATCGAAACCATTCGAGCTCAATATGCAGAGTACTTCGCCATGGAAAACCAGAGGCGACCTTCAAGAGTTGAGGCTTTGTCCGCCTTCCTCTACGGCCGTTTTATCGCCGCCATTAAAGGCGCTTCTAACAGCAATCCGGCGAACGGAAGCTCGGAATCGGAGAAGAAGATATTCTTAGTCTGCCACGCCGTGAACATCCGATCAAGACTGGACCCACCAGTTCCGGATTACGCATTCGGAAACTATTACAGAGCCACATTCGCAGTTCCGTCGGAGACGATTTTAAACGACGATTATTGCTACGATTTAGTGAAACTAGCGAGAGAAGAAATCGCCAAAATCGACAAATCGTATTTGGAAAGGCTTCAAGAAAGCAGTAAGTTCTTGGAGGCGATGAAGAAGGCGGCGGCGCAATTCTCCACCGGCGAGCTGATTTCCTGCTCGTTCACTAGCCTGTGCCGGATGCCGATTTACGACGCTGACTTCGGGTGGGGAAAACCGGCGTGGATCAGCTCGCCGGCGTTAATGTTCAAGAACCTGTTTGTGTTGATCGATAAGAAAGACGGCGATGGAGTTGACGTTTATGTTCATCTCAAGCCGGAGAACATGGAGATTCTTGAAGCCGATGAGAACATAGGTTGA

Protein sequence

MTSMHSLSINWDPRQSFIFHRFPCFSLLNSEFQSIGEIAMAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETHLSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENMEILEADENIG
Homology
BLAST of Cp4.1LG01g15840 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.0e-67
Identity = 152/439 (34.62%), Postives = 243/439 (55.35%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYS---AGSATAAEIS 99
           MA  ++++S+E ++PSSPTP   + + LS LDQV +  + P+I FY      +   A+ S
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRS 60

Query: 100 DHLKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNK 159
           ++LK+SLSTVL+ +YPLAGR+N +   +DCND+GVPF+E RV+ +LS+ +      ELN+
Sbjct: 61  ENLKRSLSTVLTQFYPLAGRININS-SVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 120

Query: 160 LLPF-------ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVS 219
            LPF       E     D+ + V+++ FECGG A+G+CISHKI+DALS    +N W A  
Sbjct: 121 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATC 180

Query: 220 RGEKEALETHLS-ASELFPPSKT---PIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAE 279
           + E + ++ +    S  FPP ++   P +    +I    V KR+  D   +E ++AQ A 
Sbjct: 181 QEETDIVQPNFDLGSHHFPPMESIPAPEFLPDENI----VMKRFVFDKEKLEALKAQLAS 240

Query: 280 YFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRL 339
             +    +  SRV+ + A ++ +FI   +   ++          K   +   AVN+RSR+
Sbjct: 241 --SATEVKNSSRVQIVIAVIWKQFIDVTRAKFDT----------KNKLVAAQAVNLRSRM 300

Query: 340 DPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLE 399
           +PP P  A GN     +AV  E    D    DLV   +  +AKID  +++ LQ+   +L+
Sbjct: 301 NPPFPQSAMGNIATMAYAVAEE----DKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLD 360

Query: 400 AMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGD 459
              +    F        SF+S CR+  YD DFGWGKP  + +  +  KNL  L+D ++ D
Sbjct: 361 YEAEPQELF--------SFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNED 410

Query: 460 GVDVYVHLKPENMEILEAD 465
           G++ ++ +  + M +L +D
Sbjct: 421 GMEAWISMAEDEMSMLSSD 410

BLAST of Cp4.1LG01g15840 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 233.8 bits (595), Expect = 4.0e-60
Identity = 152/438 (34.70%), Postives = 233/438 (53.20%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYS---AGSATAAEIS 99
           MA  +E VS+E + PSSPTP + + Y +S LDQ+ +  + P I FY      +   A+ S
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS 60

Query: 100 DHLKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSD-VMNTAFPTELN 159
            HLK+SLS VL+H+YPLAGR+N +   +DCND+GVPF+E RV  +LS  + N     +L+
Sbjct: 61  QHLKQSLSKVLTHFYPLAGRINVNS-SVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 120

Query: 160 KLLPF------ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVS 219
           + LP       +++  +DV + V+++ FECGG A+G+ +SHKI+D LS    +N W A  
Sbjct: 121 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 180

Query: 220 RGEKEALETHLS-ASELFPP-SKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYF 279
           RGE E +  +   A+  FPP   TP  +         V KR+  D   I  +RAQ +   
Sbjct: 181 RGETEIVLPNFDLAARHFPPVDNTP--SPELVPDENVVMKRFVFDKEKIGALRAQAS--- 240

Query: 280 AMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDP 339
           +   ++  SRV+ + A+++   I   +          +    K  F+V  AVN+RSR++P
Sbjct: 241 SASEEKNFSRVQLVVAYIWKHVIDVTR----------AKYGAKNKFVVVQAVNLRSRMNP 300

Query: 340 PVPDYAFGNYYRATF-AVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEA 399
           P+P YA GN     F AV +E    D    DL+   R  + K +  +   L +    L  
Sbjct: 301 PLPHYAMGNIATLLFAAVDAEW---DKDFPDLIGPLRTSLEKTEDDHNHELLKGMTCLYE 360

Query: 400 MKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDG 459
           ++         EL+  SFTS CR+  YD DFGWGKP    +     +N  +L+D + GDG
Sbjct: 361 LEPQ-------ELL--SFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDG 410

Query: 460 VDVYVHLKPENMEILEAD 465
           V+ ++ +  + M +L  +
Sbjct: 421 VEAWLPMAEDEMAMLPVE 410

BLAST of Cp4.1LG01g15840 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.7e-56
Identity = 158/471 (33.55%), Postives = 239/471 (50.74%), Query Frame = 0

Query: 44  IEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDH----- 103
           +EV+SKET+KP +PTP   R Y +S LDQ +  VY P+I FY A S   +  S H     
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 104 -LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKL 163
            LK+SLS  L H+YP+AGR+  D + +DCND G+ F E R+  R+ D M  +    L+ L
Sbjct: 69  LLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFMMKS-DAHLSLL 128

Query: 164 LPFEL---DALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVS----R 223
           LP E+   + + +  + VQ+N+F+CGG A+  CIS+KI+DA +    +   A  +    R
Sbjct: 129 LPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIARR 188

Query: 224 GEKEALET-------HLSASELFPPSK----TPIYNTRTSIFRQR-VAKRYQIDSASIET 283
           G   A  T          ++ LFPPS+       Y T+ S    + V+KR+  D+A I +
Sbjct: 189 GSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKITS 248

Query: 284 IRAQYAEYFAMENQ-RRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCH 343
            R +       + +  RP+RVE +SA ++       K A  S P          I  V H
Sbjct: 249 AREKLQSLMHDKYKCHRPTRVEVVSALIW-------KSAVKSAPPG-------SISTVTH 308

Query: 344 AVNIRSRLDPPVPDYAFGNYYRATFAV-PSETILND------------------DYCYDL 403
           A+N R ++DPP+ D +FGN      AV P+ T                      D   D 
Sbjct: 309 AMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDF 368

Query: 404 VKLAREEIAKI--DKSYLERLQESSKFLEAMKKAAAQFSTGE--LISCSFTSLCRMPIYD 463
           V L R EI K+  DK  +E++ +  KF+     + A+ S  E  + +   TS C+   Y+
Sbjct: 369 VALLRREIDKVKGDKGCMEKIIQ--KFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYE 428

BLAST of Cp4.1LG01g15840 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.3e-54
Identity = 140/436 (32.11%), Postives = 229/436 (52.52%), Query Frame = 0

Query: 44  IEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAG-SATAAEISDHLKKS 103
           +E+VSK  +KPSSPTP + RRY LS +DQ        + +FY+   S  +++I D LK S
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGDLLKNS 65

Query: 104 LSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFEL 163
           LS  L  YY  AGR+  ++ +IDCND GV F+E R++ R++D++        + +LP  +
Sbjct: 66  LSKTLVSYYQFAGRLIEND-YIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLPTRI 125

Query: 164 DAL-DDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWA-AVSRGEKEALETHL 223
            AL +D  + VQL+ F+CGG+A+G   SHK+SD +S  + +  WA + S          L
Sbjct: 126 IALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKPTPLL 185

Query: 224 SASELFPPSKTPIYNTRTSI-FRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 283
           +A  +FPP    + + ++ + F+Q + KR+   + +IE ++++  EY      ++PSRVE
Sbjct: 186 TADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEY----GIQKPSRVE 245

Query: 284 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 343
            ++AFL                A       KK + +  AVN+R  L   +P  + GN + 
Sbjct: 246 VVTAFL-------------CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIFS 305

Query: 344 ATFAVPSETILNDDYCYDLV--------KLAREEIAKIDKSYLERLQESSKFLEAMKKAA 403
             F +  E +  D+    L+        KL    I+K   +Y  ++QE +K L+      
Sbjct: 306 FYFCINDEGM--DNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLD------ 365

Query: 404 AQFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYV 463
            Q +   L +   +S CR P+YD DFGWGKP  +S      K+L +L+D  +GDG++  +
Sbjct: 366 -QLNISSLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALI 412

Query: 464 HLKPENMEILEADENI 468
            ++ + M   E +E +
Sbjct: 426 TMEEKKMAAFEKNEEL 412

BLAST of Cp4.1LG01g15840 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 6.3e-50
Identity = 143/477 (29.98%), Postives = 243/477 (50.94%), Query Frame = 0

Query: 44  IEVVSKETVKPSSPTPDNHRRYGLSFLDQ-VTVDVYNPMIYFYSAGSATAAEISDH---- 103
           +EV+SKET+KP++PTP   + + LS LDQ   +  Y P+I FY A +A +   S+H    
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 104 --LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNK 163
             LK SLS  L H+YP+AGR+  D + +DC+D G+ F + ++  ++ + M+      L++
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRM-IDNILVDCHDQGINFYKVKIRGKMCEFMSQP-DVPLSQ 128

Query: 164 LLPFELDALD---DVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEK 223
           LLP E+ +     +  + VQ+N+F+CGG A+   +SHKI+DA +    +  WA+ ++  +
Sbjct: 129 LLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSR 188

Query: 224 ----------EALETHLSASELFPP----------SKTPIYNTRTSIFRQR-VAKRYQID 283
                     + L     ++ LFPP          S+ P  +T       + V+KR+  D
Sbjct: 189 SGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRFVFD 248

Query: 284 SASIETIRAQYAEYFAMEN--QRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEK 343
            A I ++R +  +    +N   RR +RVE +++ ++       K    S PA        
Sbjct: 249 FAKITSVR-EKLQVLMHDNYKSRRQTRVEVVTSLIW-------KSVMKSTPAG------- 308

Query: 344 KIFLVCHAVNIRSRLDPPVPDYAFGN-------YYRATFAV--------------PSETI 403
            + +V HAVN+R ++DPP+ D +FGN       +  AT                  S+ +
Sbjct: 309 FLPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVV 368

Query: 404 LNDDYCYDLVKLAREEIAKI--DKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSL 463
           L++   +D +   R EI K+  DK  LE++ ++  F      +  + +  E+I+   +S 
Sbjct: 369 LHE--LHDFIAQMRSEIDKVKGDKGSLEKVIQN--FASGHDASIKKINDVEVINFWISSW 428

BLAST of Cp4.1LG01g15840 vs. NCBI nr
Match: KAG6601573.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 448/462 (96.97%), Postives = 460/462 (99.57%), Query Frame = 0

Query: 4   MHSLSINWDPRQSFIFHRFPCFSLLNSEFQSIGEIAMAVDIEVVSKETVKPSSPTPDNHR 63
           MHSLS+NWDP+QSFIFH FPCFSLLNSEFQSIGEIAMAVDI+VVSKETVKPSSPTPD+HR
Sbjct: 1   MHSLSLNWDPKQSFIFHHFPCFSLLNSEFQSIGEIAMAVDIDVVSKETVKPSSPTPDHHR 60

Query: 64  RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLKKSLSTVLSHYYPLAGRVNYDELF 123
           RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLK+SLSTVLSHYYPLAGRVNYDELF
Sbjct: 61  RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLKESLSTVLSHYYPLAGRVNYDELF 120

Query: 124 IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA 183
           IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA
Sbjct: 121 IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA 180

Query: 184 VGICISHKISDALSFFIVVNGWAAVSRGEKEALETHLSASELFPPSKTPIYNTRTSIFRQ 243
           VGICISHKISDALSFFIVVNGWAAV RGEKEALETHLSASELFPP+KTPIYNTRTSIFRQ
Sbjct: 181 VGICISHKISDALSFFIVVNGWAAVCRGEKEALETHLSASELFPPNKTPIYNTRTSIFRQ 240

Query: 244 RVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN 303
           RVAKRYQID+ASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN
Sbjct: 241 RVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN 300

Query: 304 GSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR 363
           GSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR
Sbjct: 301 GSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR 360

Query: 364 EEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPA 423
           +EIAKIDKSYLERLQESSKFLEAMKKAA+QFSTGELISCSFTSLCRMPIYDADFGWGKPA
Sbjct: 361 DEIAKIDKSYLERLQESSKFLEAMKKAASQFSTGELISCSFTSLCRMPIYDADFGWGKPA 420

Query: 424 WISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENMEILEADE 465
           WISSPAL+FKNLFVL+DKKDGDGVDVYVHLKPENMEILE+DE
Sbjct: 421 WISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENMEILESDE 462

BLAST of Cp4.1LG01g15840 vs. NCBI nr
Match: KAG7032338.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 909 bits (2349), Expect = 0.0
Identity = 447/462 (96.75%), Postives = 459/462 (99.35%), Query Frame = 0

Query: 4   MHSLSINWDPRQSFIFHRFPCFSLLNSEFQSIGEIAMAVDIEVVSKETVKPSSPTPDNHR 63
           MHSLS+NWDP+QSFIFH FPCFSLLNSEFQSIGEIAMAVDI+VVSKETVKPSSPTPD+HR
Sbjct: 1   MHSLSLNWDPKQSFIFHHFPCFSLLNSEFQSIGEIAMAVDIDVVSKETVKPSSPTPDHHR 60

Query: 64  RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLKKSLSTVLSHYYPLAGRVNYDELF 123
           RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLK+SLSTVLSHYYPLAGRVNYDELF
Sbjct: 61  RYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHLKESLSTVLSHYYPLAGRVNYDELF 120

Query: 124 IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA 183
           IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA
Sbjct: 121 IDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFELDALDDVSMGVQLNVFECGGVA 180

Query: 184 VGICISHKISDALSFFIVVNGWAAVSRGEKEALETHLSASELFPPSKTPIYNTRTSIFRQ 243
           VGICISHKISDALSFFIVVNGWAAV RGEKE LETHLSASELFPP+KTPIYNTRTSIFRQ
Sbjct: 181 VGICISHKISDALSFFIVVNGWAAVCRGEKEGLETHLSASELFPPNKTPIYNTRTSIFRQ 240

Query: 244 RVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN 303
           RVAKRYQID+ASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN
Sbjct: 241 RVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPAN 300

Query: 304 GSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR 363
           G+SESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR
Sbjct: 301 GNSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAR 360

Query: 364 EEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPA 423
           +EIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPA
Sbjct: 361 DEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPA 420

Query: 424 WISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENMEILEADE 465
           WISSPAL+FKNLFVL+DKKDGDGVDVYVHLKPENMEILE+DE
Sbjct: 421 WISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENMEILESDE 462

BLAST of Cp4.1LG01g15840 vs. NCBI nr
Match: XP_023522263.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo] >XP_023531980.1 vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855 bits (2209), Expect = 4.09e-312
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 99
           MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL
Sbjct: 1   MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 60

Query: 100 KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 159
           KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP
Sbjct: 61  KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 120

Query: 160 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 219
           FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH
Sbjct: 121 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 180

Query: 220 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 279
           LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE
Sbjct: 181 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 240

Query: 280 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 339
           ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR
Sbjct: 241 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 300

Query: 340 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 399
           ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL
Sbjct: 301 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 360

Query: 400 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 459
           ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME
Sbjct: 361 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 420

Query: 460 ILEADE 465
           ILEADE
Sbjct: 421 ILEADE 426

BLAST of Cp4.1LG01g15840 vs. NCBI nr
Match: XP_022956577.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 840 bits (2171), Expect = 2.52e-306
Identity = 416/426 (97.65%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 99
           MAVDIEVVSKETVKPSSPTPD+HRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEIS+HL
Sbjct: 1   MAVDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISNHL 60

Query: 100 KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 159
           K+SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP
Sbjct: 61  KESLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 120

Query: 160 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 219
           FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAV RGEKEALETH
Sbjct: 121 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALETH 180

Query: 220 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 279
           LSASELFPP+KTPIYNTRTSIFRQRVAKRYQID+ASIETIRAQYAEYFAMENQRRPSRVE
Sbjct: 181 LSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVE 240

Query: 280 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 339
           ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR
Sbjct: 241 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 300

Query: 340 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 399
           ATFAVPSETILNDDYCYDLVKLAR+EIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL
Sbjct: 301 ATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 360

Query: 400 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 459
           ISCSFTSLCRMPIYDADFGWGKPAWISSPAL+FKNLFVL+DKKDGDGVDVYVHLKPENME
Sbjct: 361 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENME 420

Query: 460 ILEADE 465
           ILE+DE
Sbjct: 421 ILESDE 426

BLAST of Cp4.1LG01g15840 vs. NCBI nr
Match: XP_022997942.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 839 bits (2168), Expect = 7.23e-306
Identity = 416/426 (97.65%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 99
           MAVDIEVVSKETVKPSSPTPD+HRRYGLSFLDQVTVDVYNPMIYFYSAGSATA EISDHL
Sbjct: 1   MAVDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAGEISDHL 60

Query: 100 KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 159
           KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP
Sbjct: 61  KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 120

Query: 160 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 219
           FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAV RGEKEALETH
Sbjct: 121 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALETH 180

Query: 220 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 279
           LS+SELFPPSKTPIYNTRTSIFRQRVAKRYQID+ASIETIRAQYA+YFAMENQRRPSRVE
Sbjct: 181 LSSSELFPPSKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYADYFAMENQRRPSRVE 240

Query: 280 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 339
           ALSAFLYGRFIAAIKGASNSNPANG SESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR
Sbjct: 241 ALSAFLYGRFIAAIKGASNSNPANGISESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 300

Query: 340 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 399
           ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL
Sbjct: 301 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 360

Query: 400 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 459
           ISCSFTSLCRMPIYDADFGWGKPAWISSPAL+FKNLFVL+DK DGDGVDVYVHLKPENME
Sbjct: 361 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKNDGDGVDVYVHLKPENME 420

Query: 460 ILEADE 465
           ILEADE
Sbjct: 421 ILEADE 426

BLAST of Cp4.1LG01g15840 vs. ExPASy TrEMBL
Match: A0A6J1GWR1 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111458272 PE=3 SV=1)

HSP 1 Score: 840 bits (2171), Expect = 1.22e-306
Identity = 416/426 (97.65%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 99
           MAVDIEVVSKETVKPSSPTPD+HRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEIS+HL
Sbjct: 1   MAVDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISNHL 60

Query: 100 KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 159
           K+SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP
Sbjct: 61  KESLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 120

Query: 160 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 219
           FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAV RGEKEALETH
Sbjct: 121 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALETH 180

Query: 220 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 279
           LSASELFPP+KTPIYNTRTSIFRQRVAKRYQID+ASIETIRAQYAEYFAMENQRRPSRVE
Sbjct: 181 LSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVE 240

Query: 280 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 339
           ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR
Sbjct: 241 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 300

Query: 340 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 399
           ATFAVPSETILNDDYCYDLVKLAR+EIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL
Sbjct: 301 ATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 360

Query: 400 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 459
           ISCSFTSLCRMPIYDADFGWGKPAWISSPAL+FKNLFVL+DKKDGDGVDVYVHLKPENME
Sbjct: 361 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENME 420

Query: 460 ILEADE 465
           ILE+DE
Sbjct: 421 ILESDE 426

BLAST of Cp4.1LG01g15840 vs. ExPASy TrEMBL
Match: A0A6J1K6H0 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111492737 PE=3 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 3.50e-306
Identity = 416/426 (97.65%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDHL 99
           MAVDIEVVSKETVKPSSPTPD+HRRYGLSFLDQVTVDVYNPMIYFYSAGSATA EISDHL
Sbjct: 1   MAVDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAGEISDHL 60

Query: 100 KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 159
           KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP
Sbjct: 61  KKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLP 120

Query: 160 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALETH 219
           FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAV RGEKEALETH
Sbjct: 121 FELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALETH 180

Query: 220 LSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRVE 279
           LS+SELFPPSKTPIYNTRTSIFRQRVAKRYQID+ASIETIRAQYA+YFAMENQRRPSRVE
Sbjct: 181 LSSSELFPPSKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYADYFAMENQRRPSRVE 240

Query: 280 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 339
           ALSAFLYGRFIAAIKGASNSNPANG SESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR
Sbjct: 241 ALSAFLYGRFIAAIKGASNSNPANGISESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 300

Query: 340 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 399
           ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL
Sbjct: 301 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 360

Query: 400 ISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENME 459
           ISCSFTSLCRMPIYDADFGWGKPAWISSPAL+FKNLFVL+DK DGDGVDVYVHLKPENME
Sbjct: 361 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKNDGDGVDVYVHLKPENME 420

Query: 460 ILEADE 465
           ILEADE
Sbjct: 421 ILEADE 426

BLAST of Cp4.1LG01g15840 vs. ExPASy TrEMBL
Match: A0A0A0KPY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1)

HSP 1 Score: 707 bits (1824), Expect = 8.99e-254
Identity = 342/427 (80.09%), Postives = 378/427 (88.52%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAG-SATAAEISDH 99
           MAV+IE+VSKET+KPSSPTP++ RRY LSFLDQVTVDVYNPM+YFY AG SAT AEISDH
Sbjct: 1   MAVEIEIVSKETIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDH 60

Query: 100 LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLL 159
           LK SLS VL+HYYPLAG+VNYDE FIDCND GVPFIET++NCRLSDVMNT FP+E+NK L
Sbjct: 61  LKTSLSHVLTHYYPLAGKVNYDEFFIDCNDNGVPFIETKINCRLSDVMNTPFPSEVNKFL 120

Query: 160 PFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALET 219
           PFELD LD+VSMGVQLNVFECGGV +GIC+SHKISDALS FIVVN WAA  RGEKE +  
Sbjct: 121 PFELDQLDEVSMGVQLNVFECGGVVIGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA 180

Query: 220 HLSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRV 279
           HLS++ELFPP+KT +YNTRTSIFRQRVA+RY+ID  ++E+IRA+YAE  AMENQRRPSRV
Sbjct: 181 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGENVESIRAKYAECSAMENQRRPSRV 240

Query: 280 EALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYY 339
           EALSAF+Y RFIAAIK  S+S   NG S SEKKIFLVCH+VNIRSRLDPPV DYAFGNYY
Sbjct: 241 EALSAFIYSRFIAAIKAVSSSELENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYY 300

Query: 340 RATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGE 399
           R TFAVPSE ILND+YCYDLVK AR EI KIDK YL+RLQE SKFL++MKK A QFSTGE
Sbjct: 301 RTTFAVPSENILNDNYCYDLVKQARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGE 360

Query: 400 LISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENM 459
           L+SCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFV IDKKDGDGVD+YVHLK E+M
Sbjct: 361 LVSCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHM 420

Query: 460 EILEADE 465
              E DE
Sbjct: 421 NKFEVDE 427

BLAST of Cp4.1LG01g15840 vs. ExPASy TrEMBL
Match: A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 1.64e-249
Identity = 336/427 (78.69%), Postives = 376/427 (88.06%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATA-AEISDH 99
           MA  I++VSKET+KPSSPTPD+ RRY LSFLDQVTVDVYNPM+YF+ AGS T  AEI+D 
Sbjct: 1   MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIADR 60

Query: 100 LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLL 159
           LK SLS VL+HYYPLAGR+NYDE FIDCND GVPFIETRVNC LSDVMNT FP+ELN+LL
Sbjct: 61  LKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRLL 120

Query: 160 PFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALET 219
           PFELD LDDVSMGVQLNVFECGGVAVGIC+SHKISDALSFFIVVNGWAA  RGEKEAL  
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEALRA 180

Query: 220 HLSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRV 279
           HLS++E+FPP+KT ++NTRTSIFRQRV +RY ID+A++ETIRA+YAE  AMENQRRPSRV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRPSRV 240

Query: 280 EALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYY 339
           EALS F+Y RFIAAIK  S     NGSSE+EKKIFLVCH+VNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 340 RATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGE 399
           R TFA PSE ILND+YCYDLVK  REEI KI+K YL+RLQESSKF+E+MKK A+QF TGE
Sbjct: 301 RTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGE 360

Query: 400 LISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENM 459
           L+SCSFTSLCRMPIYD DFGWG+P WISSPAL+FKNLFV IDKKDGDG+++YVHL  E+M
Sbjct: 361 LVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHM 420

Query: 460 EILEADE 465
           +  E DE
Sbjct: 421 KKFEIDE 427

BLAST of Cp4.1LG01g15840 vs. ExPASy TrEMBL
Match: A0A1S3BDS3 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 1.64e-249
Identity = 336/427 (78.69%), Postives = 376/427 (88.06%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATA-AEISDH 99
           MA  I++VSKET+KPSSPTPD+ RRY LSFLDQVTVDVYNPM+YF+ AGS T  AEI+D 
Sbjct: 1   MAFQIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIADR 60

Query: 100 LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLL 159
           LK SLS VL+HYYPLAGR+NYDE FIDCND GVPFIETRVNC LSDVMNT FP+ELN+LL
Sbjct: 61  LKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRLL 120

Query: 160 PFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEALET 219
           PFELD LDDVSMGVQLNVFECGGVAVGIC+SHKISDALSFFIVVNGWAA  RGEKEAL  
Sbjct: 121 PFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEALRA 180

Query: 220 HLSASELFPPSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQRRPSRV 279
           HLS++E+FPP+KT ++NTRTSIFRQRV +RY ID+A++ETIRA+YAE  AMENQRRPSRV
Sbjct: 181 HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRRPSRV 240

Query: 280 EALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYY 339
           EALS F+Y RFIAAIK  S     NGSSE+EKKIFLVCH+VNIRSRLDPPVPDYAFGNYY
Sbjct: 241 EALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAFGNYY 300

Query: 340 RATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGE 399
           R TFA PSE ILND+YCYDLVK  REEI KI+K YL+RLQESSKF+E+MKK A+QF TGE
Sbjct: 301 RTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQFFTGE 360

Query: 400 LISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVHLKPENM 459
           L+SCSFTSLCRMPIYD DFGWG+P WISSPAL+FKNLFV IDKKDGDG+++YVHL  E+M
Sbjct: 361 LVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLTEEHM 420

Query: 460 EILEADE 465
           +  E DE
Sbjct: 421 KKFEIDE 427

BLAST of Cp4.1LG01g15840 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-90
Identity = 182/435 (41.84%), Postives = 266/435 (61.15%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHR--RYGLSFLDQVTVDVYNPMIYFY-SAGSATAAEIS 99
           M ++I V S+E VKP SP   NH    + LSFLDQ+   ++ P ++FY +  + +  E S
Sbjct: 1   MGLEITVTSQELVKP-SPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERS 60

Query: 100 DHLKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNK 159
           DH+K SLS +L+ YYPLAGR+      + CND GV F+E + +C +S ++    P ELNK
Sbjct: 61  DHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNK 120

Query: 160 LLPFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEKEAL 219
           L PFE   + DV + VQL  FECGG+A+GI +SHK+ DALS  I VN WAA +RG+ + +
Sbjct: 121 LHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQTDEI 180

Query: 220 ET-HLSASELFPPSKTPIYNTRTSIFRQR-VAKRYQIDSASIETIRAQYAEYFAMENQRR 279
            T     +++FPP      N  T I ++  V +R+    +S+E++R    E F+   + R
Sbjct: 181 ITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLR----ERFSGNKKIR 240

Query: 280 PSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAF 339
            +RVE LS F++ RF+A    ++N +   G      KI+ + H VN+R + DP +PD  F
Sbjct: 241 ATRVEVLSVFIWSRFMA----STNHDDKTG------KIYTLIHPVNLRRQADPDIPDNMF 300

Query: 340 GNYYRATFAVPSETI-LNDDYCYDLVKLAREEIAKIDKSYLERLQESSK-FLEAMKKAAA 399
           GN  R +  VP   I  ND+    LV   REEI KID  Y+++LQE ++  LE + K A+
Sbjct: 301 GNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQAS 360

Query: 400 QFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVDVYVH 459
            F  GE++S SFTSLC+ P+Y+ADFGWGKP W++S  + +KNL   ID K+GDG++ +++
Sbjct: 361 GFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWIN 420

Query: 460 LKPENMEILEADENI 468
           L   +M   EADE +
Sbjct: 421 LDQNDMSRFEADEEL 420

BLAST of Cp4.1LG01g15840 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.5e-65
Identity = 141/439 (32.12%), Postives = 248/439 (56.49%), Query Frame = 0

Query: 44  IEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSA-GSATAAEISDHLKKS 103
           ++VVS++ +KPSSPTP++ +++ LS L+Q+   ++ PM++FYSA  S    E    LKKS
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLKKS 62

Query: 104 LSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTELNKLLPFEL 163
           LS  L+H+YPLAGR+    + IDCND+G  F+E RVN  LS+++       L +L+P  +
Sbjct: 63  LSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIPTSV 122

Query: 164 DALDDVS--MGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAV-SRGEKEALETH 223
           D+++  +  +  Q + FECG +++G+CISHK++DA S  + +  WAA+ SRG  + +   
Sbjct: 123 DSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKTIGAP 182

Query: 224 L-SASELFPP------SKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYFAMENQ 283
           +    ++FPP      S  P+      +  Q ++KR+  DS+SI+ ++A+ + +      
Sbjct: 183 VFDTVKIFPPGNFSETSPAPVVEPEI-MMNQTLSKRFIFDSSSIQALQAKASSF----EV 242

Query: 284 RRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDY 343
            +P+RVEA+SA ++   + A +  S ++          K  ++ ++V++RSR+ PP    
Sbjct: 243 NQPTRVEAVSALIWKSAMKATRTVSGTS----------KPSILANSVSLRSRVSPPFTKN 302

Query: 344 AFGNYYRATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAM----K 403
           + GN   + FA  +E  +N      LV   R+   +    ++ +L  +    E +    K
Sbjct: 303 SIGNLV-SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQK 362

Query: 404 KAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVLIDKKDGDGVD 463
           +A    ++G+     F+S CR  +Y+ DFGWGKP W+  P++  KN+  L+D K+  G++
Sbjct: 363 EAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIE 422

Query: 464 VYVHLKPENMEILEADENI 468
            +V+L  + M + E D  +
Sbjct: 423 AWVNLNEQEMNLFEQDREL 424

BLAST of Cp4.1LG01g15840 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.9e-58
Identity = 148/444 (33.33%), Postives = 230/444 (51.80%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAG-----SATAAE 99
           M  ++E++S+E VKPSSPTPD+ R   LS LD ++  +Y   + FY+A        +  E
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEE 60

Query: 100 ISDHLKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRVNCRLSDVMNTAFPTEL 159
            S  LKKSLS  L  +YPLAGR+     F++CND G  FIE RV+  LS+ +    P  L
Sbjct: 61  TSLKLKKSLSKTLPIFYPLAGRIIGS--FVECNDEGAVFIEARVDHLLSEFLKCPVPESL 120

Query: 160 NKLLPFELDALDDVS---MGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRG 219
             L+P E  + + V+   + +Q N F CGG+ + IC+SHKI+DA S  + + GWA  SRG
Sbjct: 121 ELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 180

Query: 220 EKEALETHLSASELFP------PSKTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAE 279
               L    +ASE+FP      PSK P+           V KR+  D++ I+ +RA+ + 
Sbjct: 181 LGITLIPSFTASEVFPKPLDELPSK-PMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASR 240

Query: 280 YFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRL 339
                  + P+RVEA++A L+ R +  +   S+  P         +  ++   VN+R ++
Sbjct: 241 NLV----KNPTRVEAVTA-LFWRCVTKVSRLSSLTP---------RTSVLQILVNLRGKV 300

Query: 340 DPPVPDYAFGNYYRATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLE 399
           D  + +   GN          E  +  +   D+V   R        +  E  + SS+  E
Sbjct: 301 D-SLCENTIGNMLSLMILKNEEAAI--ERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFE 360

Query: 400 AMKKAAAQFSTG-ELISCSFTSLCRMPIYDADFGWGKPAWISSPALM-FKNLFVLIDKKD 459
            +++    +  G E+      S C++ +YDADFGWGKP W++      FKNL +LID KD
Sbjct: 361 LLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKD 420

Query: 460 GDGVDVYVHLKPENMEILEADENI 468
           G+G++ ++ L  E M + E D+ +
Sbjct: 421 GEGIEAWITLTEEQMSLFECDQEL 424

BLAST of Cp4.1LG01g15840 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.8e-50
Identity = 138/446 (30.94%), Postives = 230/446 (51.57%), Query Frame = 0

Query: 44  IEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDH-LKKS 103
           +E ++KE +KPSS TP++ +   LS  D +   VY     FY+     + E S H LK S
Sbjct: 6   VETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLKTS 65

Query: 104 LSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKLLPFE 163
           L+  L+ +YPLAGR+    + IDCND G  F++ RV N  LSD + +     L +LLP  
Sbjct: 66  LAETLTKFYPLAGRIK--GVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLP-- 125

Query: 164 LDALDD--------VSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVSRGEK 223
           LD +DD          + V+   F CGG+A+G+CI+HKI+DA S    +  WAA++RGE 
Sbjct: 126 LDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGEA 185

Query: 224 EAL--ETHLSASELFPPS----KTPIYNTRTSIFRQRVAKRYQIDSASIETIRAQYAEYF 283
             +  +   +A+  +PP+    K P+      I   ++ KR+   ++ +E +R + A   
Sbjct: 186 GDIVADPVFAAANFYPPANELFKFPVDENANKI--SQITKRFVFSASKLEELRTKAA--- 245

Query: 284 AMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDP 343
           + +   RP RVE+++A L+  F+AA   +SN+N  +        + ++    N+R ++  
Sbjct: 246 SEDFVGRPKRVESVTALLWKAFVAA--ASSNTNACD--------MKVLIQPSNLRPKIPS 305

Query: 344 PVPDYAFGNYYRATFAV---PSETILNDDYCYDLVKLAREEIAKI--DKSYLERLQESSK 403
            + +   GN   ++  +     E I  +    DL K   +++  +  ++         SK
Sbjct: 306 LLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQG-DDLQNVIKEEGGSSSAMIGSK 365

Query: 404 FLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAWIS-SPALMFKNLFVLIDK 463
               M    ++ S       + +S C++P+Y+A FGWG P WI+ + A M +N+ +LID 
Sbjct: 366 LANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDS 425

Query: 464 KDGDGVDVYVHLKPENMEILEADENI 468
           KDG G++ +V L  ENM  LE +  +
Sbjct: 426 KDGQGIEAFVTLPEENMLSLEQNTEL 431

BLAST of Cp4.1LG01g15840 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 194.1 bits (492), Expect = 2.5e-49
Identity = 134/451 (29.71%), Postives = 227/451 (50.33%), Query Frame = 0

Query: 40  MAVDIEVVSKETVKPSSPTPDNHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISDH- 99
           M + +E +SKE +KPSSPTP+N +   LS  D +   VY     FY+     + E + H 
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHK 63

Query: 100 LKKSLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKL 159
           LK SLS  L+ +YPLAGR+    + +DC D G  F++ RV NC L++ +       L +L
Sbjct: 64  LKTSLSETLTKFYPLAGRIT--GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 123

Query: 160 LPFELDALDDVSMG--------VQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVS 219
           LP  LD +D+  +         V+   F CGG+A+GICI+HKI+DA S    +  WAA +
Sbjct: 124 LP--LDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 183

Query: 220 RGEKE--ALETHLSASELFPPSKTPIYN---TRTSIFRQRVAKRYQIDSASIETIRAQYA 279
           RGE +  A+E+ + A   F P     +       +  R  + KR+  +++ +E +R + A
Sbjct: 184 RGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAA 243

Query: 280 EYFAMENQRRPSRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSR 339
              + E   +P+RVE+++A ++  F+A+ K          ++  + K+ +     N+RS+
Sbjct: 244 ---SEETVDQPTRVESVTALIWKCFVASSK----------TTTCDHKVLV--QLANLRSK 303

Query: 340 LDPPVPDYAFGNYYRATFAVP---SETILNDDYCYDLVKLAREEIAKI----DKSYLERL 399
           +   + + + GN   ++  +       +  ++   DL K  +EE+  +      S     
Sbjct: 304 IPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRK-KKEELGTVILDEGGSSDSSS 363

Query: 400 QESSKFLEAMKKAAAQFSTGELISCSFTSLCRMPIYDADFGWGKPAW-ISSPALMFKNLF 459
              SK    M    ++ S       + +S C++P+Y+A FGW  P W + + + +  NL 
Sbjct: 364 MIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLA 423

Query: 460 VLIDKKDGDGVDVYVHLKPENMEILEADENI 468
           +LID KDG G++ +V L  ENM   E +  +
Sbjct: 424 MLIDSKDGQGIEAFVTLPEENMSSFEQNPEL 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2P1GIW72.0e-6734.62Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR74.0e-6034.70Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
I3PLR47.7e-5633.55(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
A0A2K8FQU53.3e-5432.11Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
Q94FT46.3e-5029.98Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
Match NameE-valueIdentityDescription
KAG6601573.10.096.97Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7032338.10.096.75Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023522263.14.09e-312100.00vinorine synthase-like [Cucurbita pepo subsp. pepo] >XP_023531980.1 vinorine syn... [more]
XP_022956577.12.52e-30697.65vinorine synthase-like [Cucurbita moschata][more]
XP_022997942.17.23e-30697.65vinorine synthase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GWR11.22e-30697.65vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111458272 PE=3 SV=1[more]
A0A6J1K6H03.50e-30697.65vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111492737 PE=3 SV=1[more]
A0A0A0KPY98.99e-25480.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1[more]
A0A5D3CZ301.64e-24978.69Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A1S3BDS31.64e-24978.69vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24430.11.3e-9041.84HXXXD-type acyl-transferase family protein [more]
AT3G26040.12.5e-6532.12HXXXD-type acyl-transferase family protein [more]
AT1G24420.12.9e-5833.33HXXXD-type acyl-transferase family protein [more]
AT3G30280.13.8e-5030.94HXXXD-type acyl-transferase family protein [more]
AT4G15390.12.5e-4929.71HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 40..235
e-value: 6.4E-59
score: 200.8
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 243..467
e-value: 1.5E-51
score: 176.6
IPR003480TransferasePFAMPF02458Transferasecoord: 43..464
e-value: 6.8E-76
score: 255.7
NoneNo IPR availablePANTHERPTHR31623:SF46ANTHRANILATE N-BENZOYLTRANSFERASEcoord: 41..466
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 41..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g15840.1Cp4.1LG01g15840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups