Cp4.1LG01g15760 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g15760
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationCp4.1LG01: 9632659 .. 9635920 (+)
RNA-Seq ExpressionCp4.1LG01g15760
SyntenyCp4.1LG01g15760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCAACTTAGTCCCATAAAACCCAAATTTGTAGCCAATTTCTTGTATACACTCAACTAACAGTTCCTTTGCCTTTCAAGCTTCACGTTGCTTCCCAGCCAGACAAGCCAAGCAACTATGAGAGGGAAACAAAGAGTGGCTTGAAAGAGTCCTGTAATTTATTGCATTGCCTTGGAAGTCCACCAAATGCTCAATAGGCCTGTACCTTTCAGACGCCTCGTCATCAAAGCTTTAGAAAAAACAACAAAAAGGGGCTTACAAGACTTCTCCAATTATAAAATAATCACACAGTAGTTGAATTCAAGATGGGGGAAGAAACTGGATAGGATCAAGATCGACGATGAAGGGGCTGGTCTTGTTGTGCTCTGTCGCCGCCCTGTGGCTTCAGGCGGCAGTGGGGCTGCCGTTGCACACCGATTCACGGTGGATCGTGGACGAACAAGGGCAGAGAGTGAAACTAAGATGCGTAAATTGGGTGTCGCATTTGGAAGCGGTGGTAGCGGAGGGGCTTTCGAAGCAGCCCATCGACGAGATTACAAATCGTATTGGGTCATTAGGATTCAACTGCGTGAGGCTCACTTGGCCACTGTTTTTAGCCACAAACGAGTCTTTGAGTTCCCTCACCGTCCGGCAGTCATTCCAGCGGCTGGGTCTGAGGGAAGCCATCGCTGGAGTCCAAGCCAATAACCCCTTCATCATCGATCTTCCGCTCATAAAGGCTTTTGAGGTAAATCGTAAATGGGGTGCTCTGTAAATGTTTGGATTTGGTGAATTATGGGTTTGATTTTGAAATTGGATTGCAGGCGGTGGTGGGGAGGTTGGGGGAGGCGGAGTTGATGGTGGTATTGGACAATCACATCAGCAAGCCAGGGTGGTGCTGCAGCAACTTCGACGGAAATGGGTTCTTCGGGGATCAGTATTTCAACCCGGAGAAATGGATCGAGGGGCTCACTAGAATGGCTACCATGTTTAACGGCGTGGCCCATGTTGTTGGTATGAGCCTTAGGAATGAGCTCCGTGGCCCAAAGCAGAATGTCAACGATTGGTACAGGTACAATACACTTCAAATTTGATTTTTAAATATATAGGTTATAATATCAATTATCTAAATTTGATTTTTAGGGTCGAAATTAGATAAAAAAAATATGTTTATTAGTAAGGGTATTCATGTCATTTGAGAATCTGGCTCATCCAAATTACGGGTTGGGTTAGATTTTTTTTAGGTTAGGTTGAATCTTTTGAAAACTGAAAAAATTCAGGTTCTTTTATTTGATGGGTCGACATATTTTTTGGTCACGTTAACCAAACCAACCCGAAAGTAAGGGTACAATCTAACCCAACCCAACTCTAGTTTTAAGATTGTTAGGAAGATTAAGAATATTTAACCTTCCTAATTGATTTTAGTGATGAATTATACTTGGGAATATTTGATATTTGAAATATATGACATAACAGAGTTTTTTAAATAATTAAAAAGAAAAACTGACTACTCAATCCAACCCAACCCAACCCAACTTGAATTGAGTTGTGAACTCTATTCCGATTGCTGAGTCACCAATTCCTCGACTAATCTAAAATTTGAGATTGGTTTAAAAGATTTTCTCAATTCAACCCTGTACTTGTTTATTAGACATAGCTTATATTTAAATTAGAAGTTTAGTTGTTATCTTCCCGTGTTATTTGGTCTCTAAACTTTCAAAATTACAATTTAATCCTTCATTTTTTTATTATTTTTTTTAAATTAAATTCAGGAGTGTACTCGGACATAATTGAAATTTTAGGATTGAAATTTTAGGATTGTAATAGATAGAAAATTCTTTTTATATCTAATACCTCAATTAAAAATCTTGAATTTATCTAGTTTAGAGATCTATTAAGAACCAATTTCAAAATTCAGATAAACTTTAGAAATGAAATTAAAAACAAAGATAAACTGGAAAGCAAAAGGAATAGGTTTGTACTAAAATCATATGAACAGAAGATTTGATCATAATTTCATATGAATGTGATGAACCAATGTTGTTGGAAGGTACATGCAAAGAGGAGCGGAGGCAGTCCACGCAGCAAACCCAGACGTTCTTGTAATTCTCTCAGGACTAAGCTTCGACAAGGACTTATCTTTTCTCAAAACCCAACCCATAAACCTCACATTCACATCCAAATTAGTGTACGAGGTTCATTGGTATGCATTTTCCGACGGCTCCTCATGGCAATCAGGCAACCCAAATCAAGTCTGCGGAAGAGTAGTAAACAACTTAATGAAAATGTCCGGATTTCTCTTAGAACAGGGGATGCCTCTGTTTCTGACCGAATTCGGAGTCGACCAAAGAGGCACCAACGTCAACGACAACAGGTATCTAAGCTGCTTCCTGAGCGTGGCAGCCGAGTACGACCTCGATTGGGCGGTGTGGACGCTCGTTGGAAGCTACTATTTGAGAGAAGGGGTTGTTGGGTTGAACGAGTTCTACGGGTTGCTTGATTGGAATTGGTGTAATCCTAGAAATTCTAGCTTCCTCCAAAGGATCTCCGCCCTGCAGACACCCTTCCAAGGCCCAGGGCTAGCCGAAAGGAGGCCTTACAGTGTAATGTTCCACCCATCAACAGGGCTATGCGTGGGGAGGAAGTCGCTATTGGACCCATTAAGATTAGGCCCATGCGCTGATTCTGATCCTTGGTACTACACGCCTCAGAAGTTTTTAACTCTCAAAGGCACGTACTTTTGCATACAAGCGCAGGACATGGGGAAGCAGCCGAGATTGGGGATAATATGTACTGTCTCAAATGCTCAATGGGATATGGTTTCTGATTCAAAGATGCATCTTTCTTCGAAACTCGACGATGGCTCCGTGGTTTGCTTGGACGTTGACCCAAGTACTAATGAAATTGTCACCAATGCTTGTAAATGCTTGAGTCGGGACTCGTCGTGTGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGTACTAGAAGCTTGGGCGCGACAAGGTCGATGATTAGTATGGTGGGTTCTTCTTTGTCGTCCAATCTTGTGCCTAAGTTGTTTGTGCAGTAATTGTTAAGGCTAAATTAAAGAAGAAAAAAAAAATGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGATGCTCTACCACTGAGCTATAGGGGCTGTTGATGTTAATCAAGATTTAAAATGTTTAATTATTATATAAATCATTACATGCAATTTCATGTCAAGAAGAAGAAGAATACGTGTCAATTATTATTGAGTTA

mRNA sequence

ATTCAACTTAGTCCCATAAAACCCAAATTTGTAGCCAATTTCTTGTATACACTCAACTAACAGTTCCTTTGCCTTTCAAGCTTCACGTTGCTTCCCAGCCAGACAAGCCAAGCAACTATGAGAGGGAAACAAAGAGTGGCTTGAAAGAGTCCTGTAATTTATTGCATTGCCTTGGAAGTCCACCAAATGCTCAATAGGCCTGTACCTTTCAGACGCCTCGTCATCAAAGCTTTAGAAAAAACAACAAAAAGGGGCTTACAAGACTTCTCCAATTATAAAATAATCACACAGTAGTTGAATTCAAGATGGGGGAAGAAACTGGATAGGATCAAGATCGACGATGAAGGGGCTGGTCTTGTTGTGCTCTGTCGCCGCCCTGTGGCTTCAGGCGGCAGTGGGGCTGCCGTTGCACACCGATTCACGGTGGATCGTGGACGAACAAGGGCAGAGAGTGAAACTAAGATGCGTAAATTGGGTGTCGCATTTGGAAGCGGTGGTAGCGGAGGGGCTTTCGAAGCAGCCCATCGACGAGATTACAAATCGTATTGGGTCATTAGGATTCAACTGCGTGAGGCTCACTTGGCCACTGTTTTTAGCCACAAACGAGTCTTTGAGTTCCCTCACCGTCCGGCAGTCATTCCAGCGGCTGGGTCTGAGGGAAGCCATCGCTGGAGTCCAAGCCAATAACCCCTTCATCATCGATCTTCCGCTCATAAAGGCTTTTGAGATAAACTTTAGAAATGAAATTAAAAACAAAGATAAACTGGAAAGCAAAAGGAATAGGTACATGCAAAGAGGAGCGGAGGCAGTCCACGCAGCAAACCCAGACGTTCTTGTAATTCTCTCAGGACTAAGCTTCGACAAGGACTTATCTTTTCTCAAAACCCAACCCATAAACCTCACATTCACATCCAAATTAGTGTACGAGGTTCATTGGTATGCATTTTCCGACGGCTCCTCATGGCAATCAGGCAACCCAAATCAAGTCTGCGGAAGAGTAGTAAACAACTTAATGAAAATGTCCGGATTTCTCTTAGAACAGGGGATGCCTCTGTTTCTGACCGAATTCGGAGTCGACCAAAGAGGCACCAACGTCAACGACAACAGGTATCTAAGCTGCTTCCTGAGCGTGGCAGCCGAGTACGACCTCGATTGGGCGGTGTGGACGCTCGTTGGAAGCTACTATTTGAGAGAAGGGGTTGTTGGGTTGAACGAGTTCTACGGGTTGCTTGATTGGAATTGGTGTAATCCTAGAAATTCTAGCTTCCTCCAAAGGATCTCCGCCCTGCAGACACCCTTCCAAGGCCCAGGGCTAGCCGAAAGGAGGCCTTACAGTGTAATGTTCCACCCATCAACAGGGCTATGCGTGGGGAGGAAGTCGCTATTGGACCCATTAAGATTAGGCCCATGCGCTGATTCTGATCCTTGGTACTACACGCCTCAGAAGTTTTTAACTCTCAAAGGCACGTACTTTTGCATACAAGCGCAGGACATGGGGAAGCAGCCGAGATTGGGGATAATATGTACTGTCTCAAATGCTCAATGGGATATGGTTTCTGATTCAAAGATGCATCTTTCTTCGAAACTCGACGATGGCTCCGTGGTTTGCTTGGACGTTGACCCAAGTACTAATGAAATTGTCACCAATGCTTGTAAATGCTTGAGTCGGGACTCGTCGTGTGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGTACTAGAAGCTTGGGCGCGACAAGGTCGATGATTAGTATGGTGGGTTCTTCTTTGTCGTCCAATCTTGTGCCTAAGTTGTTTGTGCAGTAATTGTTAAGGCTAAATTAAAGAAGAAAAAAAAAATGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGATGCTCTACCACTGAGCTATAGGGGCTGTTGATGTTAATCAAGATTTAAAATGTTTAATTATTATATAAATCATTACATGCAATTTCATGTCAAGAAGAAGAAGAATACGTGTCAATTATTATTGAGTTA

Coding sequence (CDS)

ATGAAGGGGCTGGTCTTGTTGTGCTCTGTCGCCGCCCTGTGGCTTCAGGCGGCAGTGGGGCTGCCGTTGCACACCGATTCACGGTGGATCGTGGACGAACAAGGGCAGAGAGTGAAACTAAGATGCGTAAATTGGGTGTCGCATTTGGAAGCGGTGGTAGCGGAGGGGCTTTCGAAGCAGCCCATCGACGAGATTACAAATCGTATTGGGTCATTAGGATTCAACTGCGTGAGGCTCACTTGGCCACTGTTTTTAGCCACAAACGAGTCTTTGAGTTCCCTCACCGTCCGGCAGTCATTCCAGCGGCTGGGTCTGAGGGAAGCCATCGCTGGAGTCCAAGCCAATAACCCCTTCATCATCGATCTTCCGCTCATAAAGGCTTTTGAGATAAACTTTAGAAATGAAATTAAAAACAAAGATAAACTGGAAAGCAAAAGGAATAGGTACATGCAAAGAGGAGCGGAGGCAGTCCACGCAGCAAACCCAGACGTTCTTGTAATTCTCTCAGGACTAAGCTTCGACAAGGACTTATCTTTTCTCAAAACCCAACCCATAAACCTCACATTCACATCCAAATTAGTGTACGAGGTTCATTGGTATGCATTTTCCGACGGCTCCTCATGGCAATCAGGCAACCCAAATCAAGTCTGCGGAAGAGTAGTAAACAACTTAATGAAAATGTCCGGATTTCTCTTAGAACAGGGGATGCCTCTGTTTCTGACCGAATTCGGAGTCGACCAAAGAGGCACCAACGTCAACGACAACAGGTATCTAAGCTGCTTCCTGAGCGTGGCAGCCGAGTACGACCTCGATTGGGCGGTGTGGACGCTCGTTGGAAGCTACTATTTGAGAGAAGGGGTTGTTGGGTTGAACGAGTTCTACGGGTTGCTTGATTGGAATTGGTGTAATCCTAGAAATTCTAGCTTCCTCCAAAGGATCTCCGCCCTGCAGACACCCTTCCAAGGCCCAGGGCTAGCCGAAAGGAGGCCTTACAGTGTAATGTTCCACCCATCAACAGGGCTATGCGTGGGGAGGAAGTCGCTATTGGACCCATTAAGATTAGGCCCATGCGCTGATTCTGATCCTTGGTACTACACGCCTCAGAAGTTTTTAACTCTCAAAGGCACGTACTTTTGCATACAAGCGCAGGACATGGGGAAGCAGCCGAGATTGGGGATAATATGTACTGTCTCAAATGCTCAATGGGATATGGTTTCTGATTCAAAGATGCATCTTTCTTCGAAACTCGACGATGGCTCCGTGGTTTGCTTGGACGTTGACCCAAGTACTAATGAAATTGTCACCAATGCTTGTAAATGCTTGAGTCGGGACTCGTCGTGTGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGTACTAGAAGCTTGGGCGCGACAAGGTCGATGATTAGTATGGTGGGTTCTTCTTTGTCGTCCAATCTTGTGCCTAAGTTGTTTGTGCAGTAA

Protein sequence

MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFEINFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLLDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGPCADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKLDDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSSLSSNLVPKLFVQ
Homology
BLAST of Cp4.1LG01g15760 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.7e-88
Identity = 193/526 (36.69%), Postives = 273/526 (51.90%), Query Frame = 0

Query: 21  LPLHTDSRWIVDE-QGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRL 80
           LPL T  RWIVDE  G RVKL CVNWV HLE  + EGL++ P+  + + I SLGFNCVRL
Sbjct: 29  LPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNCVRL 88

Query: 81  TWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFE---------- 140
           T+ + + T  S ++ TV Q+F RL L EA +G++ NNP ++DL  + A+           
Sbjct: 89  TYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALSEAG 148

Query: 141 ------------------------------------------------------INFRNE 200
                                                                 ++ RNE
Sbjct: 149 VMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSLRNE 208

Query: 201 IKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKLVY 260
           ++         +R+MQ GA  VH ANP VLVILSGL FD DLSFL   P+ L F  K+VY
Sbjct: 209 LRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTLPFKEKIVY 268

Query: 261 EVHWYAFSDGSSWQSGNPNQVC----GRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTN 320
           E HWY+F  G  W++G PN VC    GR  +N+  ++        PLF++EFG+DQR  N
Sbjct: 269 EGHWYSF--GVPWRTGLPNDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGIDQRYVN 328

Query: 321 VNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLR---EGVVGLNEFYGLLDWNWCNPRNSS 380
            NDNRYL+C L+  AE DLDWA+WT+ GSYY R   + V    E YG  + +W   RN  
Sbjct: 329 DNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWSRIRNPD 388

Query: 381 FLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGPCAD-SDPWYY-- 440
           F+ R+  +Q P Q P LA    Y +++HP++GLCV    + + + LG C      W Y  
Sbjct: 389 FISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCV-ESGIGNTVHLGSCQSVRSRWNYDA 448

Query: 441 TPQKFLTLKGTYFCIQAQDMGKQPRLGIICTV-SNAQWDMVSDSKMHLSSKL--DDGSVV 464
           + +  + L G+  CI  Q  G    +   C+  +N  W  VS +++ L +++   DG   
Sbjct: 449 SVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGKDGKEK 508

BLAST of Cp4.1LG01g15760 vs. NCBI nr
Match: XP_023514558.1 (uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 473/552 (85.69%), Postives = 479/552 (86.78%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII
Sbjct: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS
Sbjct: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 540

BLAST of Cp4.1LG01g15760 vs. NCBI nr
Match: KAG6601558.1 (hypothetical protein SDJN03_06791, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 466/552 (84.42%), Postives = 478/552 (86.59%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           M+GLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MRGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLKTQPINLTFT+KLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNL+KMSGFLLEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLLKMSGFLLEQGM 300

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           DDGS+VCLDVDPS+NEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGA RSMISMVGSS
Sbjct: 481 DDGSMVCLDVDPSSNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGAARSMISMVGSS 540

BLAST of Cp4.1LG01g15760 vs. NCBI nr
Match: XP_022957392.1 (uncharacterized protein LOC111458804 [Cucurbita moschata])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 467/552 (84.60%), Postives = 477/552 (86.41%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIG LGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLKTQPINLTFT+KLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           DDGSVVCLDVDPS+NEIVTNACKCLSRDSSC+PSSQWFKLVNSTRSLGA RSMISMVGSS
Sbjct: 481 DDGSVVCLDVDPSSNEIVTNACKCLSRDSSCNPSSQWFKLVNSTRSLGAARSMISMVGSS 540

BLAST of Cp4.1LG01g15760 vs. NCBI nr
Match: KAG7032321.1 (hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 920 bits (2377), Expect = 0.0
Identity = 464/552 (84.06%), Postives = 476/552 (86.23%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           M+GLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MRGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIG LGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNL+KMSGFLLEQGM
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLLKMSGFLLEQGM 300

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDS+MHLSSKL
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSRMHLSSKL 480

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           +DGSVVCLDVDPSTNEIVTNACKCLS+DSSCDPSSQWFKLVNSTRSLG TRSMISMVGSS
Sbjct: 481 EDGSVVCLDVDPSTNEIVTNACKCLSQDSSCDPSSQWFKLVNSTRSLGRTRSMISMVGSS 540

BLAST of Cp4.1LG01g15760 vs. NCBI nr
Match: XP_022997703.1 (uncharacterized protein LOC111492584 [Cucurbita maxima])

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 462/552 (83.70%), Postives = 473/552 (85.69%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII
Sbjct: 62  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 121

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 122 DLPLIKAFEAVVGWLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 181

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 182 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 241

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLK QPINLTFT+KLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM
Sbjct: 242 LSFLKNQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 301

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 302 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 361

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGR SLLDPLRLGP
Sbjct: 362 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRASLLDPLRLGP 421

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQ+MGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 422 CADSDPWYYTPQKFLTLKGTYFCIQAQEMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 481

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           D+GSVVCLDVDP+TNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRS   TRSMISMVGSS
Sbjct: 482 DNGSVVCLDVDPNTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSFDTTRSMISMVGSS 541

BLAST of Cp4.1LG01g15760 vs. ExPASy TrEMBL
Match: A0A6J1GZ00 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 PE=3 SV=1)

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 467/552 (84.60%), Postives = 477/552 (86.41%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIG LGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLKTQPINLTFT+KLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           DDGSVVCLDVDPS+NEIVTNACKCLSRDSSC+PSSQWFKLVNSTRSLGA RSMISMVGSS
Sbjct: 481 DDGSVVCLDVDPSSNEIVTNACKCLSRDSSCNPSSQWFKLVNSTRSLGAARSMISMVGSS 540

BLAST of Cp4.1LG01g15760 vs. ExPASy TrEMBL
Match: A0A6J1KEP8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=3 SV=1)

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 462/552 (83.70%), Postives = 473/552 (85.69%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII
Sbjct: 62  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 121

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPLIKAFE                                                   
Sbjct: 122 DLPLIKAFEAVVGWLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 181

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVHAANPDVLVILSGLSFDKD
Sbjct: 182 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 241

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLK QPINLTFT+KLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM
Sbjct: 242 LSFLKNQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 301

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL
Sbjct: 302 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 361

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGR SLLDPLRLGP
Sbjct: 362 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRASLLDPLRLGP 421

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           CADSDPWYYTPQKFLTLKGTYFCIQAQ+MGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL
Sbjct: 422 CADSDPWYYTPQKFLTLKGTYFCIQAQEMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 481

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 488
           D+GSVVCLDVDP+TNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRS   TRSMISMVGSS
Sbjct: 482 DNGSVVCLDVDPNTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSFDTTRSMISMVGSS 541

BLAST of Cp4.1LG01g15760 vs. ExPASy TrEMBL
Match: A0A5D3CXK4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001710 PE=3 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 2.72e-284
Identity = 391/548 (71.35%), Postives = 441/548 (80.47%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL++L +V  +   AAVGLPLHTD+RWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLLILLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 62  PIEEISNRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFII 121

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPL+KAFE                                                   
Sbjct: 122 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 181

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 182 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 241

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLK QPINLTFTSK VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+QG 
Sbjct: 242 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 301

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 302 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 361

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQTP QGPGLAERR Y+++FHP +GLCV RKSLLDPLRLGP
Sbjct: 362 LDWNWCNLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGP 421

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           C DSD WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTV+NA+WDM+SDSK+HLSSK 
Sbjct: 422 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKS 481

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 484
            +GS+VCLDVD +TNEIVTN+CKCLSRDSSCDPSSQWFKLVNSTRSLG  RSMI+M GSS
Sbjct: 482 SNGSLVCLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSS 541

BLAST of Cp4.1LG01g15760 vs. ExPASy TrEMBL
Match: A0A1S3BF06 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 2.72e-284
Identity = 391/548 (71.35%), Postives = 441/548 (80.47%), Query Frame = 0

Query: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL++L +V  +   AAVGLPLHTD+RWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLLILLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 62  PIEEISNRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFII 121

Query: 121 DLPLIKAFE--------------------------------------------------- 180
           DLPL+KAFE                                                   
Sbjct: 122 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 181

Query: 181 -------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKD 240
                        ++ RNE++   +  +   RYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 182 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 241

Query: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300
           LSFLK QPINLTFTSK VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+QG 
Sbjct: 242 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 301

Query: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 302 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 361

Query: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQTP QGPGLAERR Y+++FHP +GLCV RKSLLDPLRLGP
Sbjct: 362 LDWNWCNLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGP 421

Query: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480
           C DSD WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTV+NA+WDM+SDSK+HLSSK 
Sbjct: 422 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKS 481

Query: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 484
            +GS+VCLDVD +TNEIVTN+CKCLSRDSSCDPSSQWFKLVNSTRSLG  RSMI+M GSS
Sbjct: 482 SNGSLVCLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSS 541

BLAST of Cp4.1LG01g15760 vs. ExPASy TrEMBL
Match: A0A0A0KTH1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3 SV=1)

HSP 1 Score: 784 bits (2024), Expect = 4.50e-282
Identity = 392/543 (72.19%), Postives = 436/543 (80.29%), Query Frame = 0

Query: 1   MKGLVLL--CSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLS 60
           MKGL+LL  C  A+    AAVGLPLHTD+RWIVD  G+RVKLRCVNWVSHLEAVVAEGLS
Sbjct: 2   MKGLILLVWCVAAS----AAVGLPLHTDTRWIVDGAGERVKLRCVNWVSHLEAVVAEGLS 61

Query: 61  KQPIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPF 120
           KQPI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAG+QANNPF
Sbjct: 62  KQPIEEISNRIQWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPF 121

Query: 121 IIDLPLIKAFE------------------------------------------------- 180
           IIDLPL+KAFE                                                 
Sbjct: 122 IIDLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGL 181

Query: 181 ---------------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFD 240
                          ++ RNE++   +  +   RYMQRGAEAVH+ANPD+L+ILSGLS+D
Sbjct: 182 TRIATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSYD 241

Query: 241 KDLSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQ 300
           +DLSFLK QPINLTFTSK VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+Q
Sbjct: 242 RDLSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQ 301

Query: 301 GMPLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFY 360
           G PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE DLDWAVWTLVGSYYLREGVVGLNEFY
Sbjct: 302 GFPLFISEFGIDQRGTNVNDNRYLSCFLAVAAELDLDWAVWTLVGSYYLREGVVGLNEFY 361

Query: 361 GLLDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRL 420
           G+LDWNWCN RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHP +GLCV RKSLLDPL L
Sbjct: 362 GILDWNWCNLRNSTFLQRISALQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLTL 421

Query: 421 GPCADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSS 477
           GPC D+D WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTV+NA+WDM+SDSK+HLSS
Sbjct: 422 GPCVDTDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSS 481

BLAST of Cp4.1LG01g15760 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.6e-142
Identity = 255/515 (49.51%), Postives = 333/515 (64.66%), Query Frame = 0

Query: 22  PLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRLTW 81
           PL T SRWIVDE G RVKL C NW SHL+ VVAEGLSKQP+D +  +I  +GFNCVRLTW
Sbjct: 34  PLSTSSRWIVDENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVRLTW 93

Query: 82  PLFLATNESL-SSLTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFE----------- 141
           PL L TNE+L +++TVRQSFQ LGL + I G Q NNP IIDLPLI+A++           
Sbjct: 94  PLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGNNDV 153

Query: 142 -----------------------------------------------------INFRNEI 201
                                                                ++ RNE+
Sbjct: 154 MVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLRNEL 213

Query: 202 KNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKLVYE 261
           +   +  +   +YMQ+GAEAVH+AN  VLVILSGLSFD DLSF++++P+ L+FT KLV+E
Sbjct: 214 RGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLSFTGKLVFE 273

Query: 262 VHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTNVNDNR 321
           +HWY+FSDG+SW + NPN +CGRV+N +    G+LL QG PLFL+EFG+D+RG N NDNR
Sbjct: 274 LHWYSFSDGNSWAANNPNDICGRVLNRIGNGGGYLLNQGFPLFLSEFGIDERGVNTNDNR 333

Query: 322 YLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLLDWNWCNPRNSSFLQRISAL 381
           Y  C    AAE D+DW++W L GSYYLR+G VG+NE+YG+LD +W + RNSSFLQ+IS L
Sbjct: 334 YFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFL 393

Query: 382 QTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDP--LRLGPCADSDPWYYTPQKFLTLK 441
           Q+P QGPG      Y+++FHP TGLC+ R SL DP  L LGPC  S+PW YT +K L +K
Sbjct: 394 QSPLQGPG-PRTDAYNLVFHPLTGLCIVR-SLDDPKMLTLGPCNSSEPWSYT-KKALRIK 453

Query: 442 GTYFCIQAQDMGKQP--RLGIICTVSNAQWDMVSDSKMHLSSKLDDGSVVCLDVDPSTNE 468
               C+Q+    K P       C+ S ++W  +S S+MHL+S   + + +CLDVD + N 
Sbjct: 454 DQQLCLQSNG-PKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDVD-TANN 513

BLAST of Cp4.1LG01g15760 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 481.9 bits (1239), Expect = 6.2e-136
Identity = 246/510 (48.24%), Postives = 331/510 (64.90%), Query Frame = 0

Query: 22  PLHTDSRWIVDE--QGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRL 81
           P  TDSRWIVD+  +G+RVKL CVNW SHLE  VAEGLSKQP+D I  +I S+GFNCVRL
Sbjct: 22  PPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIAEKIVSMGFNCVRL 81

Query: 82  TWPLFLATNESLSS-LTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFE--------- 141
           TWPL+LAT+ES S+ +TVRQS ++  L EA++G Q +NP I+DLPLIKAF+         
Sbjct: 82  TWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLIKAFQEVVYCLEKH 141

Query: 142 --------------------------------------------------------INFR 201
                                                                   ++ R
Sbjct: 142 RVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMFANVSSNVVGMSLR 201

Query: 202 NEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKL 261
           NE++   +      +YM+ GAEAVH+ NP+VLVI+SGL++  DLSFL+ +P  ++F  K+
Sbjct: 202 NELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFLRERPFEVSFRRKV 261

Query: 262 VYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTNVN 321
           V+E+HWY F   ++W+  N N++CG+    +MKMSGFLLE+G+PLF++EFG+DQRG N N
Sbjct: 262 VFEIHWYGF--WNTWEGDNLNKICGKETEKMMKMSGFLLEKGIPLFVSEFGIDQRGNNAN 321

Query: 322 DNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLLDWNWCNPRNSSFLQRI 381
           DN++LSCF+++AA+ DLDW++WTL GSYY+RE  +G +E YG+LD+NW + RNS+ LQ I
Sbjct: 322 DNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGVLDFNWSSIRNSTILQMI 381

Query: 382 SALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGPCADSDPWYYTPQKFLTL 441
           SA+QTPF   GL E +P  +MFHPSTGLC+ RKSL   L+LG C  S+ W  +  + L+L
Sbjct: 382 SAIQTPF--IGLMETQPKKIMFHPSTGLCIVRKSLFQ-LKLGSCNRSESWRLSSHRVLSL 441

Query: 442 -KGTYFCIQAQDMGKQPRLGIICTVSN-AQWDMVSDSKMHLSSKLDDGSVVCLDVDPSTN 462
            +    C++A + GK  +L +  + S  ++W + SDSKM LSS   +G  VCLDVD   N
Sbjct: 442 AEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTENN 501

BLAST of Cp4.1LG01g15760 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 460.3 bits (1183), Expect = 1.9e-129
Identity = 237/510 (46.47%), Postives = 331/510 (64.90%), Query Frame = 0

Query: 22  PLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRLTW 81
           PL T+SRWI+DE+GQRVKL CVNW SHL+ VVAEGLSKQ +D++  +I ++GFNCVR TW
Sbjct: 4   PLSTNSRWIIDEKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFTW 63

Query: 82  PLFLATNESL-SSLTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFE----------- 141
           PL LATNE+L +++TVRQSFQ LGL + I+G +  NP +IDLPLI+A++           
Sbjct: 64  PLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNNV 123

Query: 142 -----------------------------------------------------INFRNEI 201
                                                                ++ RNE+
Sbjct: 124 MVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNEL 183

Query: 202 KNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKLVYE 261
           +   +      +YMQ+GAEAVH ANP+VLVILSGLS+D DLSF++++ +NLTFT KLV+E
Sbjct: 184 RGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVFE 243

Query: 262 VHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTNVNDNR 321
           +H Y+F++ ++W S NPN+ CG ++ ++    GF L +  P+FL+EFG+D RG NVNDNR
Sbjct: 244 LHRYSFTNTNTWSSKNPNEACGEILKSIENGGGFNL-RDFPVFLSEFGIDLRGKNVNDNR 303

Query: 322 YLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLLDWNWCNPRNSSFLQRISAL 381
           Y+ C L  AAE D+DW++WTL GSYYLREGVVG++EFYG+LD +W   R+ SFLQR+S +
Sbjct: 304 YIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLI 363

Query: 382 QTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLR--LGPCADSDPWYYTPQKFLTLK 441
            +P QGPG ++ + Y+++FHP TGLC+  +S+LDP +  LG C +S PW YTPQ  LTLK
Sbjct: 364 LSPLQGPG-SQSKVYNLVFHPLTGLCM-LQSILDPTKVTLGLCNESQPWSYTPQNTLTLK 423

Query: 442 GTYFCIQAQDMGKQPRLG-IICTVSN-AQWDMVSDSKMHLSSKLDDGSVVCLDVDPSTNE 462
               C+++       +L    C+  N ++W+ +S S M L++K  + S +CLDVD  TN 
Sbjct: 424 DKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNS-LCLDVD-ETNN 483

BLAST of Cp4.1LG01g15760 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-122
Identity = 229/523 (43.79%), Postives = 317/523 (60.61%), Query Frame = 0

Query: 4   LVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQPID 63
           L L  S+ +L L  A   PL T SRWIV+ +G RVKL C NW SHL+ VVAEGLS QP+D
Sbjct: 11  LFLFLSLISLTL--ATDYPLFTKSRWIVNNKGHRVKLACANWPSHLKPVVAEGLSSQPMD 70

Query: 64  EITNRIGSLGFNCVRLTWPLFLATNESLS-SLTVRQSFQRLGLREAIAGVQANNPFIIDL 123
            I+ +I  +GFNCVRLTWPL L  N++L+ ++TV+QSF+R GL   + G+  +NP+I++ 
Sbjct: 71  SISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNT 130

Query: 124 PLIKAFE----------------------------------------------------- 183
           PLI  F+                                                     
Sbjct: 131 PLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMA 190

Query: 184 -----------INFRNEIKNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLS 243
                      ++ RNE++  +       +YMQ+GAEAVH +NP+VLVILSGL+FD DLS
Sbjct: 191 TIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLS 250

Query: 244 FLKTQPINLTFTSKLVYEVHWYAFSDGS-SWQSGNPNQVCGRVVNNLMKMSGFLLEQGMP 303
           FLK +P+NL+F  KLV E+HWY+F+DG+  W+S N N  C ++ +   +  GF+L+QG P
Sbjct: 251 FLKDRPVNLSFKKKLVLELHWYSFTDGTGQWKSHNVNDFCSQMFSKERRTGGFVLDQGFP 310

Query: 304 LFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLL 363
           LFL+EFG DQRG ++  NRY++C L+ AAE DLDWAVW + G YY REG  G+ E YG+L
Sbjct: 311 LFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYGML 370

Query: 364 DWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLL--DPLRLG 423
           D NW N  N ++L+R+S +Q P  GPG+ +   +  +FHP TGLC+ RKS      L LG
Sbjct: 371 DANWHNVHNYTYLRRLSVIQPPHTGPGV-KHNHHKKIFHPLTGLCLVRKSHCHESELTLG 430

Query: 424 PCADSDPWYYTPQKFLTL-KGTYFCIQAQD-MGKQPRLGIICTVSNAQWDMVSDSKMHLS 457
           PC   +PW Y+    L + +G   C++ +  +GK  +LG ICT    + + +S +KMHLS
Sbjct: 431 PCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICT----KIEQISATKMHLS 490

BLAST of Cp4.1LG01g15760 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.5e-105
Identity = 208/505 (41.19%), Postives = 285/505 (56.44%), Query Frame = 0

Query: 22  PLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQPIDEITNRIGSLGFNCVRLTW 81
           PL T SRWIVDE+GQRVKL CVNW +HL+  VAEGLSKQP+D I+ +I S+GFNCVRLTW
Sbjct: 25  PLSTKSRWIVDEKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVRLTW 84

Query: 82  PLFLATNESLS-SLTVRQSFQRLGLREAIAGVQANNPFIIDLPLIKAFE----------- 141
           PL L TN++L+  +TV+QSF+ L L E + G+Q +NP ++ LPL  AF+           
Sbjct: 85  PLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGENGV 144

Query: 142 -----------------------------------------------------INFRNEI 201
                                                                ++ RNE 
Sbjct: 145 MVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLRNEP 204

Query: 202 KNKDKLESKRNRYMQRGAEAVHAANPDVLVILSGLSFDKDLSFLKTQPINLTFTSKLVYE 261
           +          R+M +GAEAVHAANP +LVILSG+ FD +LSFL+ + +N++FT KLV+E
Sbjct: 205 RGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTDKLVFE 264

Query: 262 VHWYAFSDG-SSWQSGNPNQVCGRVVNNLMKMSGFLLEQGMPLFLTEFGVDQRGTNVNDN 321
           +HWY+FSDG  SW+  N N  C +++  +    GFLL +G PL L+EFG DQRG +++ N
Sbjct: 265 LHWYSFSDGRDSWRKHNSNDFCVKIIEKVTHNGGFLLGRGFPLILSEFGTDQRGGDMSGN 324

Query: 322 RYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGLLDWNWCNPRNSSFLQRISA 381
           RY++C ++ AAE DLDWAVW L G YYLR                               
Sbjct: 325 RYMNCLVAWAAENDLDWAVWALTGDYYLR------------------------------- 384

Query: 382 LQTPFQGPGLAERRPYSVMFHPSTGLCV--GRKSLLDPLRLGPCADSDPWYYTPQKFLTL 441
                 GPGL   +  +++FHPSTGLCV       +  LRLGPC  SDPW + P + + L
Sbjct: 385 -----TGPGLRPNK--NLLFHPSTGLCVTNNPSDNIPTLRLGPCPKSDPWTFNPSEGI-L 444

Query: 442 KGTYFCIQAQD-MGKQPRLGIICTVSNAQWDMVSDSKMHLSSKLDDGSVVCLDVDPSTNE 458
                C++A + +G++ +LG+    S  +   +S +KMHLS K  +G ++CLDVD   N 
Sbjct: 445 WINKMCVEAPNVVGQKVKLGVGTKCS--KLGQISATKMHLSFKTSNGLLLCLDVDERDNS 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA02.7e-8836.69Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023514558.10.085.69uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo][more]
KAG6601558.10.084.42hypothetical protein SDJN03_06791, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022957392.10.084.60uncharacterized protein LOC111458804 [Cucurbita moschata][more]
KAG7032321.10.084.06hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022997703.10.083.70uncharacterized protein LOC111492584 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GZ000.084.60Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 P... [more]
A0A6J1KEP80.083.70Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=... [more]
A0A5D3CXK42.72e-28471.35Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BF062.72e-28471.35Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV... [more]
A0A0A0KTH14.50e-28272.19Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G13130.12.6e-14249.51Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.16.2e-13648.24Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.9e-12946.47Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.0e-12243.79Glycosyl hydrolase superfamily protein [more]
AT5G16700.11.5e-10541.19Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 125..145
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 21..128
e-value: 4.4E-18
score: 67.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 129..314
e-value: 5.9E-31
score: 109.8
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 8..129
coord: 131..465
NoneNo IPR availablePANTHERPTHR31263:SF44CELLULASE (GLYCOSYL HYDROLASE FAMILY 5) PROTEINcoord: 8..129
coord: 131..465
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 143..277
e-value: 2.5E-14
score: 53.4
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 330..456
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 6..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g15760.1Cp4.1LG01g15760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds