Cp4.1LG01g15640 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g15640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionribose-phosphate pyrophosphokinase 4
LocationCp4.1LG01: 9300513 .. 9302962 (-)
RNA-Seq ExpressionCp4.1LG01g15640
SyntenyCp4.1LG01g15640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTGAAGTTCTTTGCGTGCGAGATCAAGAACATCGAGAATTCTTTTCAGTGGACTGTTGAAAGCGTTCCTGGGACTGATTCTTTCTGTTCAATTCAAAAATCTGCTTCTACTTCGATCTCGATACCGACGACTACTGCCTCTCTAGAACTGACGCCTAAAAGCGACGCCAAGAAGATCTGCCTCTTGTTCTGCTAGGCGAGACGAAGATTCTTCGCAGAAAATTGCTGCTGAGTCTGATGGAATTGAGCCACGTGATGGCTTATGGAGGTTAGTTCAATTATGTAGCTATATGGAGAAGAAATCTGTAGGAATATGCTTTCAATTTATGATACAGAATTTTTTAATATGCTTTCAATTTATGGTTTCCATTTGAGACTTCAAAATGTCCCCGCAGAAAGAACGATTAAGATTGTATTGCTGCGTAGCTCAGCCCGGTGAAGTTATTTGTGTTCCTGATCGTAATGTCGAATTGTTGAGATTTTGAAAGTTCTCCAATGTTTTTAAGTTTATAGCATTTTTCACTGCGAAACAACATTGCGTATTCTGCCTTTTTAATTTAAGCATGATAAACATAAGCTGTTCGAGTTTCACAGAAGTTTATTCAAAATCAAAAGTCCTTTGGATTAATGCTTGCACAGTTCTTATATTAGGAAGTTTGAAGATGGATTACGAACATAATTATGTCCAATGCTAAAGGAATTCGTGAACAACATGTTGCCTTTCTGGCATCCTTCAAGTTCCCCTGTGTGAACTTTGAGCAACTCTCCCTTATTTATGCACTGCTGAAATTGTTCGTCTCTTCATTGACGCCTGTTCTCTCCATCTTTCCAACTGGAAATTTTGACTAAGCTTTTACTATTGCTCGGATCTCGATTATATATATGAATTTGGAGAGGAGGGCCGACTAACTGTGTACCTTTTGACATTCATGATGCAGATTCATTAACAAGCAGAACTTTACTTTTTAGAAGATAACTTGCTCTGCCTATTAGAATCCCTCACTTTTTTGAGTTTTTCCTCCTCTTCAGTGGCTGTTATTATTATTATTATTTTTTTTTTTTGTAGCAGAAGCTCTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAGTGTGCAACAGTTGCCAGATTCTGACCATGTAAGCTCTCGTNATCCCTCACTTTTTTGAGTTTTTCCTCCTCTTCAGTGGCTGTTATTTTTTTTGTAGCAGAAGCTTTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAGTGTGCAACAGTTGCCAGATTCTGACCATGTAAGCTCTCGTGTTTATGAACACGGCACGTGATATAACTGTGGGAAGGGAAAGCCCAAAAGAGACAACATCTGTTAGCGGTAGATTTTGACTGTTATAAATTATATCAGAACCAAACATGGAGCGATGTGCTAGCAAGGAGGTTGAGCCCCAAAGGGATAGATATGAGGCGGTGTGCCATCAAGGACGATAGGCTCCGAAGAGAGGTGGATTGAGGATTTTACATTGATTGGAAAAAAGAACGAGTGTCAGCGAGGACGCTGGCCCCAAAGAGAGTGGATTGTGAGACCCNGTGATGTAACTGTGGGAAGGGAAAGCCCGAAAGAGACAATATCTGTTAGCGGTAGATTTCGACGATTATAAATGATATTAGAACTAAATATGGAGCGATGTGCTAGCAGGAGGTTGAGCCCCAAAGGGATAGATATGAGGCGGTGTGCCATCAAGGACGATAGGCTCCGAAGAGAGGTGGATTGAGGATTTTACATTGATTGGAAAAAAGAACGAGTGTCAGCGAGGACGCTGGCCCCAAAGAGAGTGGATTGTGAGACCCGACGCATTTCAAAAATTTTAAGGAGAAGTTCGAGGAAAAAAGTTCAAAGAAGACAATATTTGCTAGTGGTAAGCTTCACCGGTTACCATAGCTAGCTTCAATATGCACTGCTAAAGTTGTTTTTGAATATTTCTCTCGAAANGAATTTTACATTGATTGGAGAAAAGAACGAGTGCCAGCGAGGACGCTGGCCCCAAAGAGAGTGGATTGTGAGACCCGACGCATTTCAAAAACCTTAAGGAGAAGTCCGAGGAAAAAAGTTCAAAGAAGACAATATTTGCTAGCGGTAAGCTTAAGCTGATTTTTAATATGCACTGCTAAAGTTGTTTTTGAATATTTCTCTCGAAATTGCATTGTGAGCTAAGGTTAATCTAGACTTTTGTAGTGCAATTGTGGAACATATTTTAGTTTGTAATCTCTCTCTGACGTGACAGAATTTGCTGCTTTCNGGCTTTTGTAGTGCAATTGTCGAACATATTTAAGTTTGTAATCTCTCTCTGACGTGACAGAATTTGCTGCTTTCCCGATAGCAATGGCTTTTCCGGACGATGGAGTATGGCAAGGACTTCATAAA

mRNA sequence

CTCCTGAAGTTCTTTGCGTGCGAGATCAAGAACATCGAGAATTCTTTTCAGTGGACTGTTGAAAGCGTTCCTGGGACTGATTCTTTCTGTTCAATTCAAAAATCTGCTTCTACTTCGATCTCGATACCGACGACTACTGCCTCTCTAGAACTGACGCCTAAAAGCGACGCCAAGAAGATCTGCCTCTTGCGAGACGAAGATTCTTCGCAGAAAATTGCTGCTGAGTCTGATGGAATTGAGCCACGTGATGGCTTATGGAGATGGATTACGAACATAATTATGTCCAATGCTAAAGGAATTCGTGAACAACATGTTGCCTTTCTGGCATCCTTCAAGTTCCCCTGTGTGAACTTTGAGCAACTCTCCCTTATTTATGCACTGCTGAAATTGTTCGTCTCTTCATTGACGCCTCAGAAGCTCTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAGTGTGCAACAGTTGCCAGATTCTGACCATAAGCTTTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAAATTTGCTGCTTTCCCGATAGCAATGGCTTTTCCGGACGATGGAGTATGGCAAGGACTTCATAAA

Coding sequence (CDS)

CTCCTGAAGTTCTTTGCGTGCGAGATCAAGAACATCGAGAATTCTTTTCAGTGGACTGTTGAAAGCGTTCCTGGGACTGATTCTTTCTGTTCAATTCAAAAATCTGCTTCTACTTCGATCTCGATACCGACGACTACTGCCTCTCTAGAACTGACGCCTAAAAGCGACGCCAAGAAGATCTGCCTCTTGCGAGACGAAGATTCTTCGCAGAAAATTGCTGCTGAGTCTGATGGAATTGAGCCACGTGATGGCTTATGGAGATGGATTACGAACATAATTATGTCCAATGCTAAAGGAATTCGTGAACAACATGTTGCCTTTCTGGCATCCTTCAAGTTCCCCTGTGTGAACTTTGAGCAACTCTCCCTTATTTATGCACTGCTGAAATTGTTCGTCTCTTCATTGACGCCTCAGAAGCTCTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAGTGTGCAACAGTTGCCAGATTCTGACCATAAGCTTTACTTGGGTGACAATACTTTACCATCCTTTGAAGTGGAATTCCTGCTAAAAAATAAATTTGCTGCTTTCCCGATAGCAATGGCTTTTCCGGACGATGGAGTATGGCAAGGACTTCATAAA

Protein sequence

LLKFFACEIKNIENSFQWTVESVPGTDSFCSIQKSASTSISIPTTTASLELTPKSDAKKICLLRDEDSSQKIAAESDGIEPRDGLWRWITNIIMSNAKGIREQHVAFLASFKFPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVEFLLKNSVQQLPDSDHKLYLGDNTLPSFEVEFLLKNKFAAFPIAMAFPDDGVWQGLHK
Homology
BLAST of Cp4.1LG01g15640 vs. ExPASy Swiss-Prot
Match: Q93Z66 (Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRS3 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.7e-24
Identity = 82/238 (34.45%), Postives = 120/238 (50.42%), Query Frame = 0

Query: 7   CEIKNIEN-SFQWTVESVPGTDSFCSIQKSASTSISIPTTTASLELTPKSDAKKICLLRD 66
           C ++++EN S   +++ +   D    I  ++S+ I++   T+    +    +K++CL   
Sbjct: 50  CGVRSLENHSGHRSLDFLSNGDPISLINPNSSSPITMAAATSE---SGSKSSKRVCLFHS 109

Query: 67  E---DSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKFPCVNF 126
           +   D +++I A+SD IE R   W+       N+ + NA+GIR QHVAFLASF  P V F
Sbjct: 110 DETRDLAERIVAKSDCIELRSINWKKFDDGFPNLFIQNAQGIRGQHVAFLASFSSPAVIF 169

Query: 127 EQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS------ 186
           EQLS+IYAL KLFVSS T    +    T    E E      F L   +  +P S      
Sbjct: 170 EQLSVIYALPKLFVSSFTLVLPFFPTGTSERMEDEGDVATAFTLARILSNIPTSRGGPTS 229

Query: 187 ---------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQGLHK 211
                      + Y GD  LP FE    LLK++  + P    I++AFPDDG W+  HK
Sbjct: 230 LVTFDIHALQERFYFGDTILPCFESGIPLLKSRLQSLPDSDNISIAFPDDGAWKRFHK 284

BLAST of Cp4.1LG01g15640 vs. ExPASy Swiss-Prot
Match: Q9XGA0 (Ribose-phosphate pyrophosphokinase 3, mitochondrial OS=Spinacia oleracea OX=3562 GN=PRS3 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 74/187 (39.57%), Postives = 95/187 (50.80%), Query Frame = 0

Query: 57  AKKICLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLA 116
           +KK+CL    ++   +++IAA+SD I+ R   WR       N+ +SNA+GIR +HVAFLA
Sbjct: 93  SKKVCLFYCPETKALAERIAAQSDAIQLRSISWRTFEDGFPNLFISNAQGIRGKHVAFLA 152

Query: 117 SFKFPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQL 176
           SF  P V FEQLS+IYAL KLFV+S      +    T    E E      F L   +  +
Sbjct: 153 SFSSPGVIFEQLSVIYALPKLFVASFKLVLPFFPTGTSERMEDEGDVATAFTLARILSNI 212

Query: 177 PDS---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDG 211
           P S                 + Y GDN LP FE    LLK K    P    I +AFPDDG
Sbjct: 213 PISREGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKKKLQQLPDSDNITIAFPDDG 272

BLAST of Cp4.1LG01g15640 vs. ExPASy Swiss-Prot
Match: Q8S2E5 (Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0723600 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.0e-19
Identity = 66/172 (38.37%), Postives = 85/172 (49.42%), Query Frame = 0

Query: 69  SQKIAAESDGIEPRDGLWRWIT----NIIMSNAKGIREQHVAFLASFKFPCVNFEQLSLI 128
           ++++ A +D IE R   WR       N+ +SNA  IR QHVAFLASF  P V FEQLS+I
Sbjct: 111 AEQVVARNDDIELRSISWRTFADGFPNLFISNAHTIRGQHVAFLASFSSPSVIFEQLSII 170

Query: 129 YALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS------------ 188
           YAL KLF+SS T    +    T    E E      F L   +  +P S            
Sbjct: 171 YALPKLFISSFTLILPFFPTGTSERMEDEGDVATAFTLARILSNIPISRGGPSSLVIFDI 230

Query: 189 ---DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQGLHK 211
                + Y GD+ LP FE    LLK++    P    I +AFPDDG W+  +K
Sbjct: 231 HALQERFYFGDSVLPCFESGIPLLKSRLQELPDSDNITIAFPDDGAWKRFYK 282

BLAST of Cp4.1LG01g15640 vs. ExPASy Swiss-Prot
Match: Q9XGA1 (Ribose-phosphate pyrophosphokinase 4 OS=Spinacia oleracea OX=3562 GN=PRS4 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.8e-18
Identity = 66/190 (34.74%), Postives = 93/190 (48.95%), Query Frame = 0

Query: 54  KSDAKKICL---LRDEDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVA 113
           K + K++ L   +  E+ ++K+AA+S  I  +   WR       N+ ++NA+ IR QHVA
Sbjct: 3   KPNTKQVLLFYCVEAEELARKVAAQSPLITLQSINWRSFDDGFPNLFINNAQDIRGQHVA 62

Query: 114 FLASFKFPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSV 173
           FLA+F  P V FEQLS+IYAL +LFV+S T    +    +    E E      F +   +
Sbjct: 63  FLAAFSSPAVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMARIL 122

Query: 174 QQLPDS---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFP 211
             +P S                 + Y  DN LP FE    LLK +    P    I +AFP
Sbjct: 123 SNIPVSRGGPTSVVIYDIHALQERFYFSDNVLPLFETGIPLLKQRLDQLPDADKIVVAFP 182

BLAST of Cp4.1LG01g15640 vs. ExPASy Swiss-Prot
Match: Q6ZFT5 (Ribose-phosphate pyrophosphokinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0714600 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-18
Identity = 63/175 (36.00%), Postives = 84/175 (48.00%), Query Frame = 0

Query: 66  EDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKFPCVNFEQL 125
           E+ + K+AA SD I  +   WR       N+ ++NA  IR QHVAFLASF  P V FEQ+
Sbjct: 24  EELALKVAASSDTIHLQSINWRSFDDGFPNLFINNAHDIRGQHVAFLASFSSPSVIFEQI 83

Query: 126 SLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS--------- 185
           S+I+AL KLF++S T    +    +    E E      F L   +  +P S         
Sbjct: 84  SVIFALPKLFIASFTLVLPFFPTGSFERVEEEGDVATAFTLARILSMIPKSRGGPTSVVI 143

Query: 186 ------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQGLHK 211
                   + Y GD+ LP FE    LL  +    P    I +AFPDDG W+  HK
Sbjct: 144 YDIHALQERFYFGDDVLPCFETGIPLLLQRLRQLPDADNITIAFPDDGAWKRFHK 198

BLAST of Cp4.1LG01g15640 vs. NCBI nr
Match: XP_031741409.1 (ribose-phosphate pyrophosphokinase 3, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 177 bits (449), Expect = 4.00e-50
Identity = 121/244 (49.59%), Postives = 140/244 (57.38%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN ENS+ WT+ESVPGT DSFCSIQ SASTS SIP  TAS     LELT K+DAKK
Sbjct: 45  ACEIKNFENSYHWTIESVPGTTDSFCSIQNSASTSTSIPMATASAAIAALELTSKNDAKK 104

Query: 66  ICLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL    ++   ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKALAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. NCBI nr
Match: XP_022957317.1 (ribose-phosphate pyrophosphokinase 4 [Cucurbita moschata] >KAG6601527.1 Ribose-phosphate pyrophosphokinase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032306.1 Ribose-phosphate pyrophosphokinase 3, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 177 bits (450), Expect = 5.17e-50
Identity = 119/243 (48.97%), Postives = 139/243 (57.20%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGTDSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKKI 65
           ACEIKN EN + WT+ESVPGTD F SIQ SASTS S+P   AS     LELT KSDAKKI
Sbjct: 45  ACEIKNFENPYPWTIESVPGTDPFSSIQNSASTSSSVPMAAASVAAAGLELTSKSDAKKI 104

Query: 66  CLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKF 125
           CL    ++   ++KIAAESDGIE R+  WR       NI + NA+GIR QHVAFLASF  
Sbjct: 105 CLFFCAETKILAEKIAAESDGIELRNISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFSS 164

Query: 126 PCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS- 185
           P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S 
Sbjct: 165 PTVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPISR 224

Query: 186 --------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQG 210
                           + Y GDN LP FE    LLKN+    P    +A+AFPDDG W+ 
Sbjct: 225 GGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNVAIAFPDDGAWKR 284

BLAST of Cp4.1LG01g15640 vs. NCBI nr
Match: XP_004135267.1 (ribose-phosphate pyrophosphokinase 3, mitochondrial isoform X1 [Cucumis sativus] >KGN51768.1 hypothetical protein Csa_008952 [Cucumis sativus])

HSP 1 Score: 177 bits (449), Expect = 7.44e-50
Identity = 121/244 (49.59%), Postives = 140/244 (57.38%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN ENS+ WT+ESVPGT DSFCSIQ SASTS SIP  TAS     LELT K+DAKK
Sbjct: 45  ACEIKNFENSYHWTIESVPGTTDSFCSIQNSASTSTSIPMATASAAIAALELTSKNDAKK 104

Query: 66  ICLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL    ++   ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKALAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. NCBI nr
Match: XP_038892890.1 (ribose-phosphate pyrophosphokinase 4 [Benincasa hispida])

HSP 1 Score: 177 bits (448), Expect = 1.07e-49
Identity = 119/245 (48.57%), Postives = 140/245 (57.14%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGTDSFCSIQKSASTSISIPTTTAS-------LELTPKSDAK 65
           ACEIKN ENS+QWT+ESVPGTD FCSIQ SASTS SIP   AS       LELT K++AK
Sbjct: 45  ACEIKNFENSYQWTIESVPGTDPFCSIQNSASTSNSIPMAAASTAAAAAALELTSKTEAK 104

Query: 66  KICLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASF 125
           +ICL    ++   ++KIAAESDGIE R   WR       NI + NA+GIR +HVAFLASF
Sbjct: 105 RICLFFCAETKILAEKIAAESDGIELRSISWRKFEDGFPNIFIPNAQGIRGRHVAFLASF 164

Query: 126 KFPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPD 185
             P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P 
Sbjct: 165 SSPAVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPI 224

Query: 186 S---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVW 210
           S                 + Y GDN LP FE    LLK +    P    IA+AFPDDG W
Sbjct: 225 SRGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKYRLQQLPDSDNIAIAFPDDGAW 284

BLAST of Cp4.1LG01g15640 vs. NCBI nr
Match: XP_008446171.1 (PREDICTED: ribose-phosphate pyrophosphokinase 4-like [Cucumis melo] >KAA0034283.1 ribose-phosphate pyrophosphokinase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (446), Expect = 2.07e-49
Identity = 120/244 (49.18%), Postives = 139/244 (56.97%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN EN++QWT+ESVPGT DSFCSIQ SAS S S+P   AS     LELT K+DAKK
Sbjct: 45  ACEIKNFENTYQWTIESVPGTTDSFCSIQNSASISTSVPMAAASAAVAALELTSKTDAKK 104

Query: 66  ICLL---RDEDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL      +  ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKTLAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLFVSS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFVSSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSHIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. ExPASy TrEMBL
Match: A0A6J1GYT1 (ribose-phosphate pyrophosphokinase 4 OS=Cucurbita moschata OX=3662 GN=LOC111458751 PE=3 SV=1)

HSP 1 Score: 177 bits (450), Expect = 2.50e-50
Identity = 119/243 (48.97%), Postives = 139/243 (57.20%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGTDSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKKI 65
           ACEIKN EN + WT+ESVPGTD F SIQ SASTS S+P   AS     LELT KSDAKKI
Sbjct: 45  ACEIKNFENPYPWTIESVPGTDPFSSIQNSASTSSSVPMAAASVAAAGLELTSKSDAKKI 104

Query: 66  CLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKF 125
           CL    ++   ++KIAAESDGIE R+  WR       NI + NA+GIR QHVAFLASF  
Sbjct: 105 CLFFCAETKILAEKIAAESDGIELRNISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFSS 164

Query: 126 PCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS- 185
           P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S 
Sbjct: 165 PTVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPISR 224

Query: 186 --------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQG 210
                           + Y GDN LP FE    LLKN+    P    +A+AFPDDG W+ 
Sbjct: 225 GGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNVAIAFPDDGAWKR 284

BLAST of Cp4.1LG01g15640 vs. ExPASy TrEMBL
Match: A0A0A0KQ59 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598760 PE=3 SV=1)

HSP 1 Score: 177 bits (449), Expect = 3.60e-50
Identity = 121/244 (49.59%), Postives = 140/244 (57.38%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN ENS+ WT+ESVPGT DSFCSIQ SASTS SIP  TAS     LELT K+DAKK
Sbjct: 45  ACEIKNFENSYHWTIESVPGTTDSFCSIQNSASTSTSIPMATASAAIAALELTSKNDAKK 104

Query: 66  ICLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL    ++   ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKALAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. ExPASy TrEMBL
Match: A0A5A7SUX6 (Ribose-phosphate pyrophosphokinase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002820 PE=3 SV=1)

HSP 1 Score: 176 bits (446), Expect = 1.00e-49
Identity = 120/244 (49.18%), Postives = 139/244 (56.97%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN EN++QWT+ESVPGT DSFCSIQ SAS S S+P   AS     LELT K+DAKK
Sbjct: 45  ACEIKNFENTYQWTIESVPGTTDSFCSIQNSASISTSVPMAAASAAVAALELTSKTDAKK 104

Query: 66  ICLL---RDEDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL      +  ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKTLAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLFVSS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFVSSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSHIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. ExPASy TrEMBL
Match: A0A1S3BDX8 (ribose-phosphate pyrophosphokinase 4-like OS=Cucumis melo OX=3656 GN=LOC103488976 PE=3 SV=1)

HSP 1 Score: 176 bits (446), Expect = 1.00e-49
Identity = 120/244 (49.18%), Postives = 139/244 (56.97%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGT-DSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKK 65
           ACEIKN EN++QWT+ESVPGT DSFCSIQ SAS S S+P   AS     LELT K+DAKK
Sbjct: 45  ACEIKNFENTYQWTIESVPGTTDSFCSIQNSASISTSVPMAAASAAVAALELTSKTDAKK 104

Query: 66  ICLL---RDEDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFK 125
           ICL      +  ++KIA ESDGI+ R   WR       NI + NA+GIR QHVAFLASF 
Sbjct: 105 ICLFFCPETKTLAEKIADESDGIDLRSISWRKFEDGFPNIFIPNAQGIRGQHVAFLASFS 164

Query: 126 FPCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS 185
            P V FEQLS+IYAL KLFVSS T    +    T    E E      F L   +  +P S
Sbjct: 165 SPSVIFEQLSVIYALPKLFVSSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSHIPIS 224

Query: 186 ---------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQ 210
                            + Y GDN LP FE    LLKN+    P    IA+AFPDDG W+
Sbjct: 225 RGGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNIAIAFPDDGAWK 284

BLAST of Cp4.1LG01g15640 vs. ExPASy TrEMBL
Match: A0A6J1J6T8 (ribose-phosphate pyrophosphokinase 4 OS=Cucurbita maxima OX=3661 GN=LOC111483080 PE=3 SV=1)

HSP 1 Score: 176 bits (445), Expect = 1.44e-49
Identity = 118/243 (48.56%), Postives = 138/243 (56.79%), Query Frame = 0

Query: 6   ACEIKNIENSFQWTVESVPGTDSFCSIQKSASTSISIPTTTAS-----LELTPKSDAKKI 65
           ACEIKN EN + WT+ESVPGTD F SIQ SASTS S+P   AS     LELT KSDAKKI
Sbjct: 47  ACEIKNFENPYPWTIESVPGTDPFSSIQNSASTSSSVPMAAASVAAAGLELTSKSDAKKI 106

Query: 66  CLLRDEDS---SQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKF 125
           CL    ++   ++KIAAESDGIE R+  WR       NI + NA+GIR  HVAFLASF  
Sbjct: 107 CLFFCAETKILAEKIAAESDGIELRNISWRKFEDGFPNIFIPNAQGIRGHHVAFLASFSS 166

Query: 126 PCVNFEQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS- 185
           P V FEQLS+IYAL KLF+SS T    +    T    E E      F L   +  +P S 
Sbjct: 167 PTVIFEQLSVIYALPKLFISSFTLVLPFFPTGTFERMEDEGDVATAFTLARILSNIPISR 226

Query: 186 --------------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQG 210
                           + Y GDN LP FE    LLKN+    P    +A+AFPDDG W+ 
Sbjct: 227 GGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKNRLQQLPDSDNVAIAFPDDGAWKR 286

BLAST of Cp4.1LG01g15640 vs. TAIR 10
Match: AT1G10700.1 (phosphoribosyl pyrophosphate (PRPP) synthase 3 )

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-25
Identity = 82/238 (34.45%), Postives = 120/238 (50.42%), Query Frame = 0

Query: 7   CEIKNIEN-SFQWTVESVPGTDSFCSIQKSASTSISIPTTTASLELTPKSDAKKICLLRD 66
           C ++++EN S   +++ +   D    I  ++S+ I++   T+    +    +K++CL   
Sbjct: 50  CGVRSLENHSGHRSLDFLSNGDPISLINPNSSSPITMAAATSE---SGSKSSKRVCLFHS 109

Query: 67  E---DSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKFPCVNF 126
           +   D +++I A+SD IE R   W+       N+ + NA+GIR QHVAFLASF  P V F
Sbjct: 110 DETRDLAERIVAKSDCIELRSINWKKFDDGFPNLFIQNAQGIRGQHVAFLASFSSPAVIF 169

Query: 127 EQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS------ 186
           EQLS+IYAL KLFVSS T    +    T    E E      F L   +  +P S      
Sbjct: 170 EQLSVIYALPKLFVSSFTLVLPFFPTGTSERMEDEGDVATAFTLARILSNIPTSRGGPTS 229

Query: 187 ---------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQGLHK 211
                      + Y GD  LP FE    LLK++  + P    I++AFPDDG W+  HK
Sbjct: 230 LVTFDIHALQERFYFGDTILPCFESGIPLLKSRLQSLPDSDNISIAFPDDGAWKRFHK 284

BLAST of Cp4.1LG01g15640 vs. TAIR 10
Match: AT2G42910.1 (Phosphoribosyltransferase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.3e-18
Identity = 63/178 (35.39%), Postives = 83/178 (46.63%), Query Frame = 0

Query: 63  LRDEDSSQKIAAESDGIEPRDGLWR----WITNIIMSNAKGIREQHVAFLASFKFPCVNF 122
           L  E+ ++ IAAESD I  +   WR       N+ ++NA  IR QHVAFLASF  P V F
Sbjct: 34  LECEELARNIAAESDHITLQSINWRSFADGFPNLFINNAHDIRGQHVAFLASFSSPAVIF 93

Query: 123 EQLSLIYALLKLFVSSLTPQKLYLGDNTLPSFEVE------FLLKNSVQQLPDS------ 182
           EQ+S+IY L +LFV+S T    +    +    E E      F +   V  +P S      
Sbjct: 94  EQISVIYLLPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMARIVSNIPISRGGPTS 153

Query: 183 ---------DHKLYLGDNTLPSFEVEF-LLKNKFAAFP----IAMAFPDDGVWQGLHK 211
                      + Y  D  LP FE    LL  +    P    + +AFPDDG W+  HK
Sbjct: 154 VVIYDIHALQERFYFADQVLPLFETGIPLLTKRLQQLPETEKVIVAFPDDGAWKRFHK 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z662.7e-2434.45Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9XGA02.5e-2239.57Ribose-phosphate pyrophosphokinase 3, mitochondrial OS=Spinacia oleracea OX=3562... [more]
Q8S2E52.0e-1938.37Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Oryza sativa subsp. japon... [more]
Q9XGA13.8e-1834.74Ribose-phosphate pyrophosphokinase 4 OS=Spinacia oleracea OX=3562 GN=PRS4 PE=2 S... [more]
Q6ZFT54.9e-1836.00Ribose-phosphate pyrophosphokinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
XP_031741409.14.00e-5049.59ribose-phosphate pyrophosphokinase 3, mitochondrial isoform X2 [Cucumis sativus][more]
XP_022957317.15.17e-5048.97ribose-phosphate pyrophosphokinase 4 [Cucurbita moschata] >KAG6601527.1 Ribose-p... [more]
XP_004135267.17.44e-5049.59ribose-phosphate pyrophosphokinase 3, mitochondrial isoform X1 [Cucumis sativus]... [more]
XP_038892890.11.07e-4948.57ribose-phosphate pyrophosphokinase 4 [Benincasa hispida][more]
XP_008446171.12.07e-4949.18PREDICTED: ribose-phosphate pyrophosphokinase 4-like [Cucumis melo] >KAA0034283.... [more]
Match NameE-valueIdentityDescription
A0A6J1GYT12.50e-5048.97ribose-phosphate pyrophosphokinase 4 OS=Cucurbita moschata OX=3662 GN=LOC1114587... [more]
A0A0A0KQ593.60e-5049.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598760 PE=3 SV=1[more]
A0A5A7SUX61.00e-4949.18Ribose-phosphate pyrophosphokinase 4-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDX81.00e-4949.18ribose-phosphate pyrophosphokinase 4-like OS=Cucumis melo OX=3656 GN=LOC10348897... [more]
A0A6J1J6T81.44e-4948.56ribose-phosphate pyrophosphokinase 4 OS=Cucurbita maxima OX=3661 GN=LOC111483080... [more]
Match NameE-valueIdentityDescription
AT1G10700.11.9e-2534.45phosphoribosyl pyrophosphate (PRPP) synthase 3 [more]
AT2G42910.12.3e-1835.39Phosphoribosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR10210:SF93RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 4-LIKE ISOFORM X1coord: 64..136
IPR005946Ribose-phosphate pyrophosphokinasePANTHERPTHR10210RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBERcoord: 64..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g15640.1Cp4.1LG01g15640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009165 nucleotide biosynthetic process
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004749 ribose phosphate diphosphokinase activity