Cp4.1LG01g14700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g14700
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	4-coumarate--CoA ligase-like 5
Location	Cp4.1LG01: 7535054 .. 7537769 (+)
RNA-Seq Expression	Cp4.1LG01g14700
Synteny	Cp4.1LG01g14700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTGGAATAAGAATAATATTAAACGCTCTTTATAAATTATAATTCAAAGAAATTTAACCAATAAATATGGATATATATATATATATATGAGAGTAATTATGTTGGAATTGAATCGTATAGCTTCACATCTGTTATGGCTTGACCCTTTTATGGCGGATATTGGTGTTATGGCTTGGCCCTTTTATGGCAGATATTGGCGCACAGACTCCCTCCTTCTATATTTTTAGAGAAAGAGAATTAGTATATGATTGAAGTGCATAATTCTTTTCGTATCGAGGGAATCACGGCTGATCTATCTCATGAATGTATAGATAAATGTTCGGATTTCTGCGATGGAATTATTTTTTTATAGCGCTGAATATCAAAAATTTATTAACACAAAATACAAAATTCTAGTTGAGGGAGTAGAAATTAGGTAATAAATTGGGGTGTATATATATATATATATATATATATATAATTTTAAGAAATAAATATTTTCAGCCCTAATTAAAATCTAAGCTTAAAAATGAAACTTCTTTCTAATTTTGGTATAATTTAACCATAATTTTGGCCGGCCGAGTATATATAAATTTGTTCAAAAAATAATTAAAACCCCACATTTCGTTGTCTTGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNACGAAAATAAATAAATAAATAAATAAATAAAGGAAACGTCGATTATGTCGCCCAAAGGCATCACAAGTTTGTCATCCTGTTTCTGATACCTTTTGCACTCACTGATAACCGATTTTTGAACCTTCACTCGTCGACGCAAAAATATGTCCCGCGAAGAATCCTGCACTGCTCCGGCCGCCGTAGACTTTCGGCCGATGAACTCTCCGGGTGGTTACGATGTGAATACTGCGGTTTATCATTCACTCGTCCAGCTTGATGAAGCTCCTAAGATCTCCACCAGTCCAGACCTCGACACGGCTACTTTCGTTTTATCGCAGTTTCCGACGGCCGAATCGCGAGTTGCTCTTATCGACTCGGTCACGAGCTCGCGAGTTACTTATGGAAAACTCAGTGCGTCGACTCGTTCCCTCGCTTACGGACTTTATCACGCACTTGGAGTTCGAAAAGGCGATGTGGTGTTCGTTTTATCCGCGAATTCTGTTTTGTATCCGGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCTAATCCTGTAAATACTGCGTCGGAAATCGGAAAGCAAGTGAGCGATTCATGTGCGAAACTCGCTATCTCGGCTCCCGAGGAGCTTCACAAATTGATTCCGACTGGAATACCGACGATTCTAACTACTCGGCCTTCTTATGGCGATGTTTTGTCGGTTGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCAAATGCCGAAAACGAAAATCACTCAGTCGGATACAGCGGCTATCTTGTACTCGTCGGGAACTACCGGGACGAGTAAAGGTGTTGTTCTAACGCATTCAAATCTTATCTCAGTTATCGAACTTCTAACCTGGTACGTGGACGCGAGTTCCTCTCAAGACGACGTGTTTTTGTGCTTCGTTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGACTCGGACTCTTCTGCAGAGGAACTACCATTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCTATTGAAACGTACAAAGTCAATAACATACCGGCAGTACCTCCGGTGATCTTAGGGCTAGTGAAATCGAGCAGTGGTTCAGATTTATCATCGCTGAGACGTATTGGATCTGGAGCTGCACCGCTGGGAAAAGACGTAGAAGAAGCTTTCCGAGAGAAATTTCCATGGGTGGAGCTTCGGCCAGGATATGGCTTAACAGAGAGTACCGGCGCAGCGACTTGCATGATTACAGACAAAGACGCAAGAGCTCATCCAGGTACTACAACATTAGCAATCACCAATTCAAATTAATTCTTAACTCTTTCATTTGTGGATCTGAAATTAGGTATTAGATCGAACAAGTGACTACAAATTTACTGTTGAATCCAAAATTGTTGTAAATTTAATCATTGATTCACATTCCTAACAAGGTTCATGTGGAATGTTGATGCCGAGATTTTGTGCAAAGATCGTAGACATTGAAACAGGAGAGGGACTACCGCCGATGAAGGAAGGCGAAGTGTGGCTGAAGAGTCCAACAATTATGAAAGAATATCTCAGAAACAAGGAAGCAACAGAAGCCACCATGGATAAAGAAGGATGGATGAAAACAGGAGATCTCGGATACATAGACGAAGATGGCTTCCTTTACATCGTTGATCGAATCAAGGAACTGATCAAACACAATGGATATCAGGTACAAACAAACGCCTCACCTCAATTATATCGTATCATACTCTGATTAAGCTCAAGAACTTCAAAATTTTCAACGAAGGTTGCTCCAGCTGAACTGGAAACAATTCTTTTGAGCCATACAGAAATTCTAGATGCTGCCGTTATACCGTGAGTTTAATCTCTTTCTCCTAATTTTCTTTTCTCTTTAATGTGTTAGATGAACATACCACTTTGAGCATAAGCCTTTCTTTTGGGCTTCCCAAGAGGCCTACATACTAACGTAGATAGTATTCTTCACTCATAAACTCAAGATCATTCCCTAAATAATGAACATACCACTTTGAGCATAAGCCTTTCTTTTGGGCTTCCCAAGAGGCCTA

mRNA sequence

ATGAAATCCTGCACTGCTCCGGCCGCCGTAGACTTTCGGCCGATGAACTCTCCGGGTGGTTACGATGTGAATACTGCGGTTTATCATTCACTCGTCCAGCTTGATGAAGCTCCTAAGATCTCCACCAGTCCAGACCTCGACACGGCTACTTTCGTTTTATCGCAGTTTCCGACGGCCGAATCGCGAGTTGCTCTTATCGACTCGGTCACGAGCTCGCGAGTTACTTATGGAAAACTCAGTGCGTCGACTCGTTCCCTCGCTTACGGACTTTATCACGCACTTGGAGTTCGAAAAGGCGATGTGGTGTTCGTTTTATCCGCGAATTCTGTTTTGTATCCGGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCTAATCCTGTAAATACTGCGTCGGAAATCGGAAAGCAAGTGAGCGATTCATGTGCGAAACTCGCTATCTCGGCTCCCGAGGAGCTTCACAAATTGATTCCGACTGGAATACCGACGATTCTAACTACTCGGCCTTCTTATGGCGATGTTTTGTCGGTTGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCAAATGCCGAAAACGAAAATCACTCAGTCGGATACAGCGGCTATCTTGTACTCGTCGGGAACTACCGGGACGAGTAAAGGTGTTGTTCTAACGCATTCAAATCTTATCTCAGTTATCGAACTTCTAACCTGGTACGTGGACGCGAGTTCCTCTCAAGACGACGTTGCTCCAGCTGAACTGGAAACAATTCTTTTGAGCCATACAGAAATTCTAGATGCTGCCGTTATACCGTGAGTTTAATCTCTTTCTCCTAATTTTCTTTTCTCTTTAATGTGTTAGATGAACATACCACTTTGAGCATAAGCCTTTCTTTTGGGCTTCCCAAGAGGCCTACATACTAACGTAGATAGTATTCTTCACTCATAAACTCAAGATCATTCCCTAAATAATGAACATACCACTTTGAGCATAAGCCTTTCTTTTGGGCTTCCCAAGAGGCCTA

Coding sequence (CDS)

ATGAAATCCTGCACTGCTCCGGCCGCCGTAGACTTTCGGCCGATGAACTCTCCGGGTGGTTACGATGTGAATACTGCGGTTTATCATTCACTCGTCCAGCTTGATGAAGCTCCTAAGATCTCCACCAGTCCAGACCTCGACACGGCTACTTTCGTTTTATCGCAGTTTCCGACGGCCGAATCGCGAGTTGCTCTTATCGACTCGGTCACGAGCTCGCGAGTTACTTATGGAAAACTCAGTGCGTCGACTCGTTCCCTCGCTTACGGACTTTATCACGCACTTGGAGTTCGAAAAGGCGATGTGGTGTTCGTTTTATCCGCGAATTCTGTTTTGTATCCGGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCTAATCCTGTAAATACTGCGTCGGAAATCGGAAAGCAAGTGAGCGATTCATGTGCGAAACTCGCTATCTCGGCTCCCGAGGAGCTTCACAAATTGATTCCGACTGGAATACCGACGATTCTAACTACTCGGCCTTCTTATGGCGATGTTTTGTCGGTTGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCAAATGCCGAAAACGAAAATCACTCAGTCGGATACAGCGGCTATCTTGTACTCGTCGGGAACTACCGGGACGAGTAAAGGTGTTGTTCTAACGCATTCAAATCTTATCTCAGTTATCGAACTTCTAACCTGGTACGTGGACGCGAGTTCCTCTCAAGACGACGTTGCTCCAGCTGAACTGGAAACAATTCTTTTGAGCCATACAGAAATTCTAGATGCTGCCGTTATACCGTGA

Protein sequence

MKSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSVEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDASSSQDDVAPAELETILLSHTEILDAAVIP

Homology

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BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot
Match: Q84P25 (4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.0e-32
Identity = 88/224 (39.29%), Postives = 139/224 (62.05%), Query Frame = 0


Query: 20  GYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKL 79
           G+  +T++++S     E   +  +  LD  +F+ SQ      +   +D+VT  R+++ +L
Sbjct: 26  GFCESTSIFYS---KREPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPEL 85

Query: 80  SASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 139
                 +A G  +ALGVRKG+VV +LS NS+L+P++ L+V+S+GA+ITTANP+NT+ EI 
Sbjct: 86  WLGVERVA-GCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 145

Query: 140 KQVSDSCAKLAISAPEELHKLIPT---GIPTIL-----TTRPSYGDVLSVEELIESCIES 199
           KQ+ DS   LA +  + + KL       +P +L         SYGD + +   +E+ IE+
Sbjct: 146 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 205

Query: 200 --FEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIE 234
              E   K ++ Q DTAA+LYSSGTTGTSKGV+L+H NLI++++
Sbjct: 206 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQ 243

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BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 7.7e-32
Identity = 88/221 (39.82%), Postives = 130/221 (58.82%), Query Frame = 0


Query: 16  NSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVT 75
           N P  +   T +Y S       P     P+LD  + + S     ++  ALIDS+T   ++
Sbjct: 20  NPPFWFSSKTGIYTSKFPSLHLP---VDPNLDAVSALFSHKHHGDT--ALIDSLTGFSIS 79

Query: 76  YGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTA 135
           + +L    +S+A G+YH LGVR+GDVV ++  NSV +P+I L+++S+GA++TT NP ++ 
Sbjct: 80  HTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSL 139

Query: 136 SEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSVE--ELIESCIESFE 195
            EI KQVS+    LA ++ E + KL   G+  I  +     D + +E  +      ESF 
Sbjct: 140 GEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFG 199

Query: 196 QMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIEL 235
            +PK  I Q D AAI+YSSGTTG SKGV+LTH NLI+ +EL
Sbjct: 200 FVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMEL 235

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BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.0e-31
Identity = 79/221 (35.75%), Postives = 133/221 (60.18%), Query Frame = 0


Query: 20  GYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKL 79
           GY  + ++++S  +  + P+   +  +D  TF+ S+      ++A ID+ T   +T+ +L
Sbjct: 14  GYCKSNSIFYSKREPVQLPQ---NHSVDVTTFISSR--AHHGKIAFIDAATGRHLTFPQL 73

Query: 80  SASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 139
             +  S+A  L  A+G+RKGDV+ +LS NS+ +PV+CLAV+S+GA+ITT NP+NT  EI 
Sbjct: 74  WRAVDSVATCL-SAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIA 133

Query: 140 KQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSVEELIESCIESFEQMPKT- 199
           KQ++DS   LA + P+ + K+  + +P ++        +     ++ S  E   + P   
Sbjct: 134 KQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPN 193

Query: 200 ----KITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELL 236
               ++ Q DTA +LYSSGTTG SKGVV +H NLI++++ +
Sbjct: 194 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTI 228

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BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 8.5e-31
Identity = 88/227 (38.77%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 15  MNSPGGY-DVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSR 74
           +NS  G+ + N+  Y     +   P    +P LD  TF+ SQ      R+A ID+ T   
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP----NPSLDVTTFISSQ--AHRGRIAFIDASTGQN 63

Query: 75  VTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVN 134
           +T+ +L  +  S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+N
Sbjct: 64  LTFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 135 TASEIGKQVSDSCAKLAISAPEELHKLIPTG--IPTILTTR---PSYGDVLSVEELIESC 194
           T++EI KQ+ DS   LA +  + L K+      +P +L       S GDV  + E+++  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK- 183

Query: 195 IESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELL 236
            E      K ++ Q DTA +LYSSGTTG SKGV+ +H NLI++++ +
Sbjct: 184 -EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTI 221

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot
Match: Q84P26 (4-coumarate--CoA ligase-like 8 OS=Arabidopsis thaliana OX=3702 GN=4CLL8 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 83/198 (41.92%), Postives = 124/198 (62.63%), Query Frame = 0


Query: 40  ISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKG 99
           + +   LD  TF+ SQ  T   + A ID+ T  R+++  L  +   +A  L H +G+R+G
Sbjct: 33  LPSKESLDITTFISSQ--TYRGKTAFIDAATDHRISFSDLWMAVDRVADCLLHDVGIRRG 92

Query: 100 DVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHK 159
           DVV VLS N++  P++CL+V+S+GAV+TTANP+NTASEI +Q++DS  KLA + PE   K
Sbjct: 93  DVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASEILRQIADSNPKLAFTTPELAPK 152

Query: 160 LIPTGIPTIL-----TTRPSYG--DVLSVEELIESCIESFEQMPKTKITQSDTAAILYSS 219
           +  +GI  +L     T R   G   V ++ E+++   E   Q  + ++ + DTA +LYSS
Sbjct: 153 IASSGISIVLERVEDTLRVPRGLKVVGNLTEMMKK--EPSGQAVRNQVHKDDTAMLLYSS 212

Query: 220 GTTGTSKGVVLTHSNLIS 231
           GTTG SKGV  +H NLI+
Sbjct: 213 GTTGRSKGVNSSHGNLIA 226

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BLAST of Cp4.1LG01g14700 vs. NCBI nr
Match: XP_023523152.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468 bits (1205), Expect = 7.33e-161
Identity = 246/247 (99.60%), Postives = 247/247 (100.00%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. NCBI nr
Match: XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 459 bits (1181), Expect = 3.18e-157
Identity = 240/247 (97.17%), Postives = 244/247 (98.79%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRP+NSPGGYDVNTAVYHSLVQ DEAPKISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTG+PTILTTRPSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIESFEQMPKTKI QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. NCBI nr
Match: KAG7031989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 458 bits (1179), Expect = 6.39e-157
Identity = 241/247 (97.57%), Postives = 244/247 (98.79%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRPMNSPGGYDVNTAVYHSLVQLDEA KISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPMNSPGGYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTGIPTILTTRPSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIE+FEQMPKTKI QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. NCBI nr
Match: XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 456 bits (1173), Expect = 5.35e-156
Identity = 239/247 (96.76%), Postives = 243/247 (98.38%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRPMNSPGGYDVNTAVYHS VQLDEAPKISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTGIPTILTT+PSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIESFEQMP+TKI QSD AAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. NCBI nr
Match: KAG6601192.1 (4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 455 bits (1170), Expect = 1.48e-155
Identity = 240/247 (97.17%), Postives = 243/247 (98.38%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRPMNSPG YDVNTAVYHSLVQLDEA KISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPMNSPGVYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTGIPTILTTRPSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIE+FEQMPKTKI QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL
Match: A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)

HSP 1 Score: 459 bits (1181), Expect = 1.54e-157
Identity = 240/247 (97.17%), Postives = 244/247 (98.79%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRP+NSPGGYDVNTAVYHSLVQ DEAPKISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTG+PTILTTRPSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIESFEQMPKTKI QSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL
Match: A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)

HSP 1 Score: 456 bits (1173), Expect = 2.59e-156
Identity = 239/247 (96.76%), Postives = 243/247 (98.38%), Query Frame = 0


Query: 2   KSCTAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAES 61
           +SCTAPAAV+FRPMNSPGGYDVNTAVYHS VQLDEAPKISTSPDLDTATFVLSQFPTAES
Sbjct: 5   ESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFPTAES 64

Query: 62  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 121
           RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS
Sbjct: 65  RVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLS 124

Query: 122 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSV 181
           IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKL PTGIPTILTT+PSYGDVLSV
Sbjct: 125 IGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGDVLSV 184

Query: 182 EELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 241
           EELIESCIESFEQMP+TKI QSD AAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA
Sbjct: 185 EELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVDA 244

Query: 242 SSSQDDV 248
           SSSQDDV
Sbjct: 245 SSSQDDV 251

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL
Match: A0A1S4DW24 (4-coumarate--CoA ligase-like 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 385 bits (990), Expect = 1.26e-129
Identity = 206/248 (83.06%), Postives = 222/248 (89.52%), Query Frame = 0


Query: 2   KSC-TAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAE 61
           +SC T PAA++F+P NSPGGY+VNTAVYHSL+  DEAP IST  DLDTAT+VLSQFPTAE
Sbjct: 5   ESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQFPTAE 64

Query: 62  SRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVL 121
           SRVALIDSVTS RVTYG+L  S RSLA GLYHALGVRKGDVVFVLSAN VLYPVICLAVL
Sbjct: 65  SRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVL 124

Query: 122 SIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLS 181
           SIGAVITTANP+NTASEIGKQV DS AKLA+SAPEEL KL PTGIPTILTTR S+ D LS
Sbjct: 125 SIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFSDALS 184

Query: 182 VEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVD 241
           VEELIESC ES E +PKT++TQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW VD
Sbjct: 185 VEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVD 244

Query: 242 ASSSQDDV 248
           ++SSQ DV
Sbjct: 245 STSSQHDV 252

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL
Match: A0A5A7SZP5 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002300 PE=4 SV=1)

HSP 1 Score: 385 bits (990), Expect = 5.48e-129
Identity = 206/248 (83.06%), Postives = 222/248 (89.52%), Query Frame = 0


Query: 2   KSC-TAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAE 61
           +SC T PAA++F+P NSPGGY+VNTAVYHSL+  DEAP IST  DLDTAT+VLSQFPTAE
Sbjct: 5   ESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQFPTAE 64

Query: 62  SRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVL 121
           SRVALIDSVTS RVTYG+L  S RSLA GLYHALGVRKGDVVFVLSAN VLYPVICLAVL
Sbjct: 65  SRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVL 124

Query: 122 SIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLS 181
           SIGAVITTANP+NTASEIGKQV DS AKLA+SAPEEL KL PTGIPTILTTR S+ D LS
Sbjct: 125 SIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFSDALS 184

Query: 182 VEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVD 241
           VEELIESC ES E +PKT++TQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW VD
Sbjct: 185 VEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVD 244

Query: 242 ASSSQDDV 248
           ++SSQ DV
Sbjct: 245 STSSQHDV 252

[Close]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL
Match: A0A1S3BFZ3 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 385 bits (990), Expect = 1.33e-128
Identity = 206/248 (83.06%), Postives = 222/248 (89.52%), Query Frame = 0


Query: 2   KSC-TAPAAVDFRPMNSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAE 61
           +SC T PAA++F+P NSPGGY+VNTAVYHSL+  DEAP IST  DLDTAT+VLSQFPTAE
Sbjct: 5   ESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQFPTAE 64

Query: 62  SRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVL 121
           SRVALIDSVTS RVTYG+L  S RSLA GLYHALGVRKGDVVFVLSAN VLYPVICLAVL
Sbjct: 65  SRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVL 124

Query: 122 SIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLS 181
           SIGAVITTANP+NTASEIGKQV DS AKLA+SAPEEL KL PTGIPTILTTR S+ D LS
Sbjct: 125 SIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFSDALS 184

Query: 182 VEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWYVD 241
           VEELIESC ES E +PKT++TQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW VD
Sbjct: 185 VEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTWCVD 244

Query: 242 ASSSQDDV 248
           ++SSQ DV
Sbjct: 245 STSSQHDV 252

[Close]

BLAST of Cp4.1LG01g14700 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-33
Identity = 88/224 (39.29%), Postives = 139/224 (62.05%), Query Frame = 0


Query: 20  GYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKL 79
           G+  +T++++S     E   +  +  LD  +F+ SQ      +   +D+VT  R+++ +L
Sbjct: 26  GFCESTSIFYS---KREPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPEL 85

Query: 80  SASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 139
                 +A G  +ALGVRKG+VV +LS NS+L+P++ L+V+S+GA+ITTANP+NT+ EI 
Sbjct: 86  WLGVERVA-GCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 145

Query: 140 KQVSDSCAKLAISAPEELHKLIPT---GIPTIL-----TTRPSYGDVLSVEELIESCIES 199
           KQ+ DS   LA +  + + KL       +P +L         SYGD + +   +E+ IE+
Sbjct: 146 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 205

Query: 200 --FEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIE 234
              E   K ++ Q DTAA+LYSSGTTGTSKGV+L+H NLI++++
Sbjct: 206 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQ 243

HSP 2 Score: 43.1 bits (100), Expect = 4.1e-04
Identity = 35/98 (35.71%), Postives = 47/98 (47.96%), Query Frame = 0


Query: 173 PSYGDVLSVEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVI 232
           P  G VL V +  E  I S   M      +  TA+ + S G   T     +     + V+
Sbjct: 399 PDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVV 458

Query: 233 ELLTWYVDASSSQDDVAPAELETILLSHTEILDAAVIP 271
           + L   +  +  Q  VAPAELE +LL+H EI DAAVIP
Sbjct: 459 DRLKELIKCNGYQ--VAPAELEALLLAHPEIADAAVIP 494

[Close]

BLAST of Cp4.1LG01g14700 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 139.0 bits (349), Expect = 5.4e-33
Identity = 88/221 (39.82%), Postives = 130/221 (58.82%), Query Frame = 0


Query: 16  NSPGGYDVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVT 75
           N P  +   T +Y S       P     P+LD  + + S     ++  ALIDS+T   ++
Sbjct: 20  NPPFWFSSKTGIYTSKFPSLHLP---VDPNLDAVSALFSHKHHGDT--ALIDSLTGFSIS 79

Query: 76  YGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTA 135
           + +L    +S+A G+YH LGVR+GDVV ++  NSV +P+I L+++S+GA++TT NP ++ 
Sbjct: 80  HTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSL 139

Query: 136 SEIGKQVSDSCAKLAISAPEELHKLIPTGIPTILTTRPSYGDVLSVE--ELIESCIESFE 195
            EI KQVS+    LA ++ E + KL   G+  I  +     D + +E  +      ESF 
Sbjct: 140 GEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFG 199

Query: 196 QMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIEL 235
            +PK  I Q D AAI+YSSGTTG SKGV+LTH NLI+ +EL
Sbjct: 200 FVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMEL 235

[Close]

BLAST of Cp4.1LG01g14700 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 135.6 bits (340), Expect = 6.0e-32
Identity = 88/227 (38.77%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 15  MNSPGGY-DVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSR 74
           +NS  G+ + N+  Y     +   P    +P LD  TF+ SQ      R+A ID+ T   
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP----NPSLDVTTFISSQ--AHRGRIAFIDASTGQN 63

Query: 75  VTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVN 134
           +T+ +L  +  S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+N
Sbjct: 64  LTFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 135 TASEIGKQVSDSCAKLAISAPEELHKLIPTG--IPTILTTR---PSYGDVLSVEELIESC 194
           T++EI KQ+ DS   LA +  + L K+      +P +L       S GDV  + E+++  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK- 183

Query: 195 IESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELL 236
            E      K ++ Q DTA +LYSSGTTG SKGV+ +H NLI++++ +
Sbjct: 184 -EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTI 221

HSP 2 Score: 39.3 bits (90), Expect = 5.9e-03
Identity = 32/98 (32.65%), Postives = 46/98 (46.94%), Query Frame = 0


Query: 173 PSYGDVLSVEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVI 232
           P  G +L  ++  E  ++    M      +  T++ L S G   T     +     I V+
Sbjct: 378 PVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVV 437

Query: 233 ELLTWYVDASSSQDDVAPAELETILLSHTEILDAAVIP 271
           + L   +     Q  VAPAELE +LL+H EI DAAVIP
Sbjct: 438 DRLKELIKYKGYQ--VAPAELEALLLTHPEITDAAVIP 473

[Close]

BLAST of Cp4.1LG01g14700 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 135.6 bits (340), Expect = 6.0e-32
Identity = 88/227 (38.77%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 15  MNSPGGY-DVNTAVYHSLVQLDEAPKISTSPDLDTATFVLSQFPTAESRVALIDSVTSSR 74
           +NS  G+ + N+  Y     +   P    +P LD  TF+ SQ      R+A ID+ T   
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP----NPSLDVTTFISSQ--AHRGRIAFIDASTGQN 63

Query: 75  VTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVN 134
           +T+ +L  +  S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+N
Sbjct: 64  LTFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 135 TASEIGKQVSDSCAKLAISAPEELHKLIPTG--IPTILTTR---PSYGDVLSVEELIESC 194
           T++EI KQ+ DS   LA +  + L K+      +P +L       S GDV  + E+++  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK- 183

Query: 195 IESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELL 236
            E      K ++ Q DTA +LYSSGTTG SKGV+ +H NLI++++ +
Sbjct: 184 -EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTI 221

HSP 2 Score: 39.3 bits (90), Expect = 5.9e-03
Identity = 32/98 (32.65%), Postives = 46/98 (46.94%), Query Frame = 0


Query: 173 PSYGDVLSVEELIESCIESFEQMPKTKITQSDTAAILYSSGTTGTSKGVVLTHSNLISVI 232
           P  G +L  ++  E  ++    M      +  T++ L S G   T     +     I V+
Sbjct: 378 PVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVV 437

Query: 233 ELLTWYVDASSSQDDVAPAELETILLSHTEILDAAVIP 271
           + L   +     Q  VAPAELE +LL+H EI DAAVIP
Sbjct: 438 DRLKELIKYKGYQ--VAPAELEALLLTHPEITDAAVIP 473

[Close]

BLAST of Cp4.1LG01g14700 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 8.7e-31
Identity = 83/198 (41.92%), Postives = 124/198 (62.63%), Query Frame = 0


Query: 40  ISTSPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKG 99
           + +   LD  TF+ SQ  T   + A ID+ T  R+++  L  +   +A  L H +G+R+G
Sbjct: 33  LPSKESLDITTFISSQ--TYRGKTAFIDAATDHRISFSDLWMAVDRVADCLLHDVGIRRG 92

Query: 100 DVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHK 159
           DVV VLS N++  P++CL+V+S+GAV+TTANP+NTASEI +Q++DS  KLA + PE   K
Sbjct: 93  DVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASEILRQIADSNPKLAFTTPELAPK 152

Query: 160 LIPTGIPTIL-----TTRPSYG--DVLSVEELIESCIESFEQMPKTKITQSDTAAILYSS 219
           +  +GI  +L     T R   G   V ++ E+++   E   Q  + ++ + DTA +LYSS
Sbjct: 153 IASSGISIVLERVEDTLRVPRGLKVVGNLTEMMKK--EPSGQAVRNQVHKDDTAMLLYSS 212

Query: 220 GTTGTSKGVVLTHSNLIS 231
           GTTG SKGV  +H NLI+
Sbjct: 213 GTTGRSKGVNSSHGNLIA 226

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g14700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84P25	2.0e-32	39.29	4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=...	[more]
Q84P24	7.7e-32	39.82	4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=...	[more]
M4IQR7	5.0e-31	35.75	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q84P21	8.5e-31	38.77	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]
Q84P26	1.2e-29	41.92	4-coumarate--CoA ligase-like 8 OS=Arabidopsis thaliana OX=3702 GN=4CLL8 PE=2 SV=...	[more]

BLAST of Cp4.1LG01g14700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523152.1	7.33e-161	99.60	4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957594.1	3.18e-157	97.17	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
KAG7031989.1	6.39e-157	97.57	4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022994494.1	5.35e-156	96.76	4-coumarate--CoA ligase-like 5 [Cucurbita maxima]	[more]
KAG6601192.1	1.48e-155	97.17	4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cp4.1LG01g14700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H2E6	1.54e-157	97.17	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=...	[more]
A0A6J1K303	2.59e-156	96.76	4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ...	[more]
A0A1S4DW24	1.26e-129	83.06	4-coumarate--CoA ligase-like 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348942...	[more]
A0A5A7SZP5	5.48e-129	83.06	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BFZ3	1.33e-128	83.06	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348942...	[more]

BLAST of Cp4.1LG01g14700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20480.1	1.4e-33	39.29	AMP-dependent synthetase and ligase family protein	[more]
AT4G19010.1	5.4e-33	39.82	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	6.0e-32	38.77	OPC-8:0 CoA ligase1	[more]
AT1G20510.2	6.0e-32	38.77	OPC-8:0 CoA ligase1	[more]
AT5G38120.1	8.7e-31	41.92	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 60..236 e-value: 2.8E-33 score: 115.2
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 32..260 e-value: 1.2E-42 score: 148.1
None	No IPR available	PANTHER	PTHR24096:SF376	AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATED	coord: 13..248
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 13..248
None	No IPR available	PANTHER	PTHR24096:SF376	AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATED	coord: 248..270
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 248..270
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 55..242
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 208..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g14700.1	Cp4.1LG01g14700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009698	phenylpropanoid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016207	4-coumarate-CoA ligase activity