Cp4.1LG01g14270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g14270
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Cp4.1LG01: 7816590 .. 7820342 (+)
RNA-Seq Expression	Cp4.1LG01g14270
Synteny	Cp4.1LG01g14270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGATGTGAATTTACGGAAATGCCCATCTGCAATTTCAATGTAACGTGCGGTATACAGCACCACTGGTTCTCGGCTCGACCCGAAGGTTCAATCCCCTAAACTGAAAATGGTTGCGATCTATAAATCCTCATTTCAAACGCCTCCGACGGCGTTTCAAACTTCTACAGAGAGTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTACTCTCAATTTCTCTACGCCTCTGATTGAGTTCTGAAATTTCAATCCGATTTTAAAATTAATTCTTCGATTCAGTTCGATTGTGGCTTTATTGAGAATCTGTTAATCTTATTCTTCGAAAATTCGTGGCGAAAAGAAAATGGCGGAGAGATCTGGATCTCTTCTCCCTTCTTTGCATCTTATGGTTCCTCTGGAGATTTCGGAACTCGTCTTTTCGTTTACCGGATTAGGAAGACTTTGAACCTATCTTGATTTCTTTTTTTTTTTTATTTTTATAATTTTTTGTCGTATGAACTTGAATAATTAACCATCCACTTTACAAATATCCGACTTCATTAGTTTTCCTCAACTAATTTAATTGTCATTAAGAACTCGTGAAGTCAACTTTGTGTATAATAATAATTTTGGAATTATTATTATATGAAGTCATGTACAAGAAAGAACAAACTGCACGTTTCTGCTCAACAGTTTTCTAAGAAATTTGTTTCAATAATCACTAGATGATTTGCACTTATTGTACCTTTCATTCATGCGACAATTATTTTTGAATCAATTCGAGTATTCTTCTCAAATTATAGAAGCTATTCCTGTATTATTTCTCCAACCAATTGCATCTGAGGGGAACTAGAAATGATAACAAAAGTTTTGGAAGATCATATGAATCATTTAGAGAACAATGAAAAGTAATATAAACAATTTCAAAAACAACAAAACACCTAATCTTATATTAAATATATCATTGAATTCAAAAGCTTAAACGGATAGACAATATGAATTACTTACTATCATGAAATTCTTTGCAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTGTGTTTTAGAACCCTTCCTTTCTTCATTTCATAAAAGCTACTTATGTTTATGCTTTACACAATTAAGTTAGGCTCATACTACTATTGTATTAGTTCCCTTAAGTTCTGATTCTGTTTATCACTACAATAGTAGAAGGTAAAGCAGTTGGATTTTGTTCTTTACCTGATCTTAATAATGACCATTTATACTTGTGTGTTTTTATTTATTAAAATTAAAATACTTTCTATGTTATGAAATTGAATTGAATTAAATTAAAAATGTTATTGAAACCTTAATCTGGAAAATTAGTCTAAAACTCCTAGGTTATATATTTGTTGTTTTAGAAAAAAACGCTCGTTCATGCAAGTGTTTCTATGGACTTTGTCGTCGACCAAATTGGGTTGTTCGTATTTGATAATAATATTGATTATCATCCTCCGTTGATAGCATTCGGCCCTAGTATAGAAGAGGAGTATCTAAAAAAATTCGATGATCCGAAAAACTCGATCAACCCATCCAAACCTATACAAATGTGTAATAAAATAAAATAAAAATTTATAGTTAGGTTCATTATTTTAAAAAAAAAAAAAAAATTATACAGAGTCTAATCCAACCCATTAACACATACATTCTATTTTTTTATTTAAAAAAAAAAAACTAATTTCTTAGAAAATAAATATATGTAAATTTTGTTTACTCTTTTTTTATAAAAATTAAAAATTAAGAGGATAAAAATTGTTATTTTCTAGATTTATAAATAAGGTTGCAAACTATCAAAAATAAAAATATTTTTAGAATATTCAGCTAAAATAAATCATAAATAATACTAGAAAAAAGAAAAAAATAATAAAAAAAACAAGAAATGTATTATATATATATATATACACTTAATTATGGAATGGCCACACACTAAGAAAGAAAAGGACATGAAGGAGATTTTTTTTTTTATAAATTGCAGAGCGATGAGGCAATCGTCGTTCACCATTAAATTTCCATCCTTCTCTGCAACTCACGATTTCAAGAGCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTACGTCTACTTAACTAATTAGATCCTCTCGATTCTGTCTGTTCTGGAAGTTAATTAGCGAAATCGAAACTCGATTATTTCAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

mRNA sequence

ATGAAAGATTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTAGCGATGAGGCAATCGTCCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

Coding sequence (CDS)

ATGAAAGATTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTAGCGATGAGGCAATCGTCCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

Protein sequence

MKDFLAVFLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF

Homology

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BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.0e-18
Identity = 44/105 (41.90%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 175 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 235 SQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPF 261

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BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 55/172 (31.98%), Postives = 88/172 (51.16%), Query Frame = 0


Query: 110 VLKAALTLHFFKRIFEVLFIHKYSSKM-VFDTAIKISFSYCSSTVIMIYTQHLSQGLPEP 169
           +L A   +H+  R     F+ +    M +    + I F  C+  +   Y  H +    + 
Sbjct: 86  ILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDW 145

Query: 170 PIDLK-NIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIE 229
             D +  IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E
Sbjct: 146 VTDPRFLIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIME 205

Query: 230 FFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           + G+A  S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF
Sbjct: 206 WCGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPF 257

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 72/247 (29.15%), Postives = 114/247 (46.15%), Query Frame = 0


Query: 43  MGMAGISEIKGKHLQYSKFWNANSAPKAPS----EEPKLSSKYGMLLAYTPAFLAGAASF 102
           +   G+  +   + +YS  W+    P  P+    E P ++  + +     PA    AA  
Sbjct: 22  VAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMA--WPLYECIRPA----AARL 81

Query: 103 WFFPSDNERILVLKAALTLHFFKR--IFEVLFIHKYSSKMVFDTAIKISF----SYCSST 162
              P+      VL A   +H+ +R  +F VL I      ++F   +   F     Y  S 
Sbjct: 82  GNLPN-----RVLLAMFLIHYVQRTLVFPVL-IRGGKPTLLFTFVLAFLFCTLNGYLQSR 141

Query: 163 VIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGL 222
            +  +  +    +  P       G AL+L+G++ N +   +L   RKPGETGYKIP+GGL
Sbjct: 142 YLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGL 201

Query: 223 FSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSH 280
           F  V   +Y  E++E+ GFA  S  L  + F+  T   L  R+    +WY+ KFE++P  
Sbjct: 202 FEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKT 253

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BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 45/104 (43.27%), Postives = 63/104 (60.58%), Query Frame = 0


Query: 176 GIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFIS 235
           G AL+L+G++ N +   +L   RKPGETGYKIP+GGLF  V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 236 QELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
             L  + F+  T   L  R+    +WY  KFE++P   K L+PF
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPF 257

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot
Match: A5PJS2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 8.6e-17
Identity = 56/176 (31.82%), Postives = 88/176 (50.00%), Query Frame = 0


Query: 110 VLKAALTLHFFKRIFEVLFIHKYSSKMVFDTAIKISFSYCS------STVIMIYTQHLSQ 169
           +L A   +H+ +R     F+ +    M    A  ++F +C+      S  +  Y  +   
Sbjct: 84  ILLAMFLVHYAQRSLVFPFLIRGGKPMPL-YAFLLAFIFCTYNGYLQSRYLSQYAVYADD 143

Query: 170 GLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLF 229
            L +P       G AL+LIG++ N +   +L   RKPGETGYKIP+GGLF  +   +Y  
Sbjct: 144 WLSDPRF---LTGSALWLIGMLINIHSDHVLRNLRKPGETGYKIPRGGLFEYISAANYFG 203

Query: 230 EIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           E++E+ G+A  S  +    F+  T   L  R+    KWY  KFE++P   K ++PF
Sbjct: 204 EVVEWCGYALASWSIQGWAFAVFTFCVLFTRAQQHHKWYHEKFEDYPKFRKIMIPF 255

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BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: KAG6601243.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 865 bits (2234), Expect = 3.46e-313
Identity = 468/555 (84.32%), Postives = 478/555 (86.13%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEET 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYC STVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETG  IP                                 C S     ++    
Sbjct: 181 KTRKPGETGNSIP---------------------------------CASAPAPPFI---- 240

Query: 256 YATRKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPF 315
                W           ++  +P  +E   QRRRPPPPP PPP  SSGPSPMPTFLFSPF
Sbjct: 241 -----W-----------LEGAMPQGNE---QRRRPPPPPLPPP--SSGPSPMPTFLFSPF 300

Query: 316 SSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLLCYVPAF 375
           SS FLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLLCYVPAF
Sbjct: 301 SSPFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLLCYVPAF 360

Query: 376 AVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIRVSI 435
           AVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEV+FVHKYSNKMGLSDAIRVSI
Sbjct: 361 AVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYSNKMGLSDAIRVSI 420

Query: 436 TYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREYR 495
           TYVLLTASTI SQMVS NLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREYR
Sbjct: 421 TYVLLTASTIYSQMVSQNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREYR 480

Query: 496 IPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYVSKF 555
           IPRGGMFEVIVCPHYLFEMVEFLG+AFVSQTLFPMVFAFGSALFL+GRSYATRNWYVSKF
Sbjct: 481 IPRGGMFEVIVCPHYLFEMVEFLGLAFVSQTLFPMVFAFGSALFLVGRSYATRNWYVSKF 497

Query: 556 EDFPRHVKAILPFVF 570
           EDFPRHVKAILPFVF
Sbjct: 541 EDFPRHVKAILPFVF 497

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 608 bits (1568), Expect = 6.78e-211
Identity = 325/580 (56.03%), Postives = 410/580 (70.69%), Query Frame = 0


Query: 14  AAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGK-HLQYSKFWNAN-SAPKAP 73
           AAM  +SA LF PPPS  VNVMTVVSS  + + G+SE +G  HLQYSKFWNAN SA K+ 
Sbjct: 2   AAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKSS 61

Query: 74  SEEP-KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIH 133
           + E  K+SS+ GMLL Y PA LAGA SFW FP+D+ RIL+LK+ALTLHF KR FEVLFIH
Sbjct: 62  NPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFIH 121

Query: 134 KYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYH 193
           KYS KM  D+A+ IS SY SST +MIY Q LS+ LPEP IDLK IGI+LFLIGIIGNFYH
Sbjct: 122 KYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFYH 181

Query: 194 HFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIY 253
           H++LS+TRK GETGYKIPKGGLF +VICPHYLFEI  +FGFA ISQ +Y LCF+  TA+Y
Sbjct: 182 HYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMY 241

Query: 254 LAGRSYATRKWYVSKFENFPSHVKALLPFSD------EAIVQRRR----------PPPPP 313
           L GRSYATRKWY+SKFE+FP H+KAL+PF        E + +  R               
Sbjct: 242 LGGRSYATRKWYLSKFEDFPQHIKALVPFHHWFSARPEGVSKFYREFLAVFLIWF----- 301

Query: 314 PPPPPPSSGPSPMPTFLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFW---- 373
                 S+    +  FLF P +STF+  ++ ++  ++ +   SE  G+HLQYSKFW    
Sbjct: 302 ------SAAMGILSAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANS 361

Query: 374 SPKTSSNKIHLPSRSAMLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFK 433
           SPK  S +  L S+  MLL Y PAF   + S +      + + + RI ++ + L +HFFK
Sbjct: 362 SPKAPSEETKLSSKYGMLLAYTPAFLAGAASFWF-----FPSDNERILVLKAALTLHFFK 421

Query: 434 RIFEVLFVHKYSNKMGLSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYW 493
           RIFEVLF+HKYS+KM    AI++S +Y L T   I +Q +S  L EP IDL+  G+A++ 
Sbjct: 422 RIFEVLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFL 481

Query: 494 IGITGNLYHHVLLAKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPM 553
           IGI GN YHH LL+K RK G+  Y+IP+GG+F +++CPHYLFE++EF G AF+SQ L+P+
Sbjct: 482 IGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPL 541

Query: 554 VFAFGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF 570
            F+ G+A++L GRSYATR WYVSKFE+FP HVKA+LPFVF
Sbjct: 542 CFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 565

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: XP_023528760.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531 bits (1367), Expect = 4.46e-185
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 256 YATRKWYVSKFENFPSHVKALLPF 279
           YATRKWYVSKFENFPSHVKALLPF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 525 bits (1353), Expect = 5.95e-183
Identity = 261/264 (98.86%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYC STVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 256 YATRKWYVSKFENFPSHVKALLPF 279
           YATRKWYVSKFENFPSHVKALLPF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 525 bits (1351), Expect = 1.20e-182
Identity = 260/264 (98.48%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYC STVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 256 YATRKWYVSKFENFPSHVKALLPF 279
           YATRKWYVSKFENFPSHVKALLPF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 2.88e-183
Identity = 261/264 (98.86%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYC STVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 256 YATRKWYVSKFENFPSHVKALLPF 279
           YATRKWYVSKFENFPSHVKALLPF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 525 bits (1351), Expect = 5.80e-183
Identity = 260/264 (98.48%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 195
           MVFDTAIKISFSYC STVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 196 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 255
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 256 YATRKWYVSKFENFPSHVKALLPF 279
           YATRKWYVSKFENFPSHVKALLPF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL
Match: A0A4D6M1Q7 (Enoyl reductase OS=Vigna unguiculata OX=3917 GN=DEO72_LG5g2622 PE=3 SV=1)

HSP 1 Score: 535 bits (1377), Expect = 6.09e-183
Identity = 287/556 (51.62%), Postives = 376/556 (67.63%), Query Frame = 0


Query: 21  AFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP--KLS 80
           +F+F PPPS  V+ ++VVS  ++  AG+SEI+GKHL YSKFWNAN     PS E   KLS
Sbjct: 8   SFIFAPPPSLVVSTLSVVSLVSLANAGLSEIRGKHLNYSKFWNAN-----PSSEKQIKLS 67

Query: 81  SKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVF 140
           SK GMLL YTPAFLA  ASFW FP +  R  +L++A+TLHFFKR FEVLFIHKYS  M+ 
Sbjct: 68  SKTGMLLLYTPAFLAALASFWIFPHEGLRSTLLQSAVTLHFFKRDFEVLFIHKYSGGMLL 127

Query: 141 DTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTR 200
           D+AI I+ SY  S   M+Y QHL+QGLPEPPI+L   GI +F++GIIGNFYHH+LLS  R
Sbjct: 128 DSAIPITLSYFLSAATMVYAQHLTQGLPEPPINLLYPGIVMFVVGIIGNFYHHYLLSNLR 187

Query: 201 KPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYAT 260
             GE  YKIPKGGLF +VICPHYLFEII+F+G +FISQ LY  CF+ GT  YL GRSY+T
Sbjct: 188 AKGEKEYKIPKGGLFGIVICPHYLFEIIQFYGISFISQTLYAFCFTLGTTFYLLGRSYST 247

Query: 261 RKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFSST 320
           RKW   + E            +  AIV                   S + +F+F P  S 
Sbjct: 248 RKWSEERVE-----------LAYTAIVM------------------SVLLSFMFPPPPSV 307

Query: 321 FLLALSSLTMLSILVLATS---EFLGRHLQYSKFWSPKTSSNK-IHLPSRSAMLLCYVPA 380
               +S LT++S ++L +S   E  G+HL YSKFW+   S+ K I LPS+  MLL Y PA
Sbjct: 308 ---VVSGLTVISFVILVSSFLSEIKGKHLNYSKFWNANPSAQKQIKLPSKVGMLLLYTPA 367

Query: 381 FAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIRVS 440
           F +A ++ F +  P+    S R  ++ S +  HFFKR+FEVLF+HKYS  M L  AI ++
Sbjct: 368 F-LAGLASFWIFPPQ----SLRSIILQSAVTFHFFKRVFEVLFIHKYSGGMTLESAIPIT 427

Query: 441 ITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREY 500
           ++Y L  A+   SQ +++ L EP ++L   G  ++ + I  N YHH LL+ LRKEG++EY
Sbjct: 428 LSYFLSAATMTYSQHLTNGLPEPPMNLLYPGTVIFVVAIICNFYHHYLLSNLRKEGEKEY 487

Query: 501 RIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYVSK 560
           +IP+GG+F +++CPHYLFE++ F GI F+SQTL+   FA G+ L+L+GRSY+TR WYVSK
Sbjct: 488 KIPKGGLFGLVICPHYLFEIIVFYGIFFISQTLYAFSFAIGTNLYLLGRSYSTRKWYVSK 521

Query: 561 FEDFPRHVKAILPFVF 570
           FEDFP++VKA++PFVF
Sbjct: 548 FEDFPQNVKAVIPFVF 521

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL
Match: A0A6J1GYE2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458608 PE=3 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 2.26e-172
Identity = 257/264 (97.35%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 307 MPTFLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSA 366
           MPTFLFSPFSS FLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSA
Sbjct: 1   MPTFLFSPFSSPFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSA 60

Query: 367 MLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMG 426
           MLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEV+FVHKY NKMG
Sbjct: 61  MLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYGNKMG 120

Query: 427 LSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKL 486
           LS+AIRVSITYVLLTASTI SQMVS NLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKL
Sbjct: 121 LSEAIRVSITYVLLTASTIYSQMVSQNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKL 180

Query: 487 RKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYA 546
           RKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLG+AFVSQTLFPMVFAFGSALFLIGRSYA
Sbjct: 181 RKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGLAFVSQTLFPMVFAFGSALFLIGRSYA 240

Query: 547 TRNWYVSKFEDFPRHVKAILPFVF 570
           TRNWYVSKFEDFPRHVKAILPFVF
Sbjct: 241 TRNWYVSKFEDFPRHVKAILPFVF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL
Match: A0A103Y9N2 (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_016565 PE=3 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 7.80e-169
Identity = 275/568 (48.42%), Postives = 365/568 (64.26%), Query Frame = 0


Query: 17  GILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPK 76
            +L  FLFP P S FV  M VV+ +A+  A   EIKGKHL+YSKF NA    K   ++ K
Sbjct: 5   SVLLNFLFPSPSSWFVTAMLVVNLSALTDAAYMEIKGKHLRYSKFLNAGGVAK--KQDMK 64

Query: 77  LSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKM 136
           LSS+ GMLL YTPAFL G ASF+  P  + R ++L +ALTLHFFKR+ EVLF+HKYS  M
Sbjct: 65  LSSRNGMLLFYTPAFLVGVASFFVVPYQDLRFVLLASALTLHFFKRVLEVLFLHKYSGSM 124

Query: 137 VFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSK 196
             ++ I I+ SY SST  MIY Q LS+ +PEP +DLK +GI LF IG IGNFYHH LL+ 
Sbjct: 125 ALESPIIITMSYTSSTATMIYAQFLSRDVPEPTVDLKYVGIGLFFIGTIGNFYHHCLLAN 184

Query: 197 TRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSY 256
            R  G+  YKIP+GGLF LVICPHYLFEI+ + G + ISQ  Y   F+ GT  YL GRSY
Sbjct: 185 LRNKGDKEYKIPQGGLFGLVICPHYLFEILAYAGISSISQTPYAFAFTLGTIFYLIGRSY 244

Query: 257 ATRKWYVSKF-ENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSG--------PSPM 316
            TR WY+SKF + FP  VKAL+P+ + A   RR P          ++         PS  
Sbjct: 245 GTRGWYLSKFGDKFPKDVKALIPYCEFANNLRRTP----------NTLRLIIKMAIPSIF 304

Query: 317 PTFLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWS----PKTSSNKIHLPS 376
              LF P SS F+  ++ +   S+      E  G+H+QYSKF +     K    ++ L S
Sbjct: 305 LNLLFPPPSSLFVTTMTVVGFSSLTNAGYKEIKGQHMQYSKFLNVGVVEKEKEKEMKLSS 364

Query: 377 RSAMLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSN 436
           ++ MLL Y P+F V   S F      + +   R  L+ S L +HFFKR+ EVLFVHKYS 
Sbjct: 365 KNGMLLFYAPSFLVGLTSFF-----AFPHEDLRFGLLVSALTVHFFKRVVEVLFVHKYSG 424

Query: 437 KMGLSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLL 496
            M L  AI V ++Y   TA+ I +Q++S ++ EP +DL+  G+ ++ IG  GN YHH LL
Sbjct: 425 SMALESAIIVPLSYAFSTATMIYTQILSRDVPEPLLDLKYVGVGLFIIGEIGNFYHHWLL 484

Query: 497 AKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGR 556
           A LRK+GD+EY+IP+GG+F++++CPHYLFE++ ++GIA +SQTL+   F  G+  +L GR
Sbjct: 485 ANLRKKGDKEYKIPKGGLFDLVICPHYLFEILAYVGIASISQTLYAFSFTLGTIFYLTGR 544

Query: 557 SYATRNWYVSKFED-FPRHVKAILPFVF 570
           SY TR WY+SKF D FP++VKA +P++F
Sbjct: 545 SYTTREWYLSKFGDKFPKNVKAFIPYIF 555

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.3e-84
Identity = 151/266 (56.77%), Postives = 201/266 (75.56%), Query Frame = 0


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEE- 75
           M ++++F++PPPPS  +N M+VV   A+   G SEI+G HL+YSKF  ++S+P+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 76  PKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSS 135
             +SS+ GMLL YTPAFLA A+SF+  PSD+ R L+LK+AL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 136 KMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLL 195
            M  D+A+ IS SY SST +M+Y+Q+L+ GL EP  D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 196 SKTRK-PGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAG 255
           +K RK  G+  YKIPKGGLF ++ICPHYLFEI+ F+ F  ISQ +Y   F+ GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 256 RSYATRKWYVSKFENFPSHVKALLPF 280
           RSYATR WY+SKF++FP H+KAL+PF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPF 266

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 2.7e-13
Identity = 40/106 (37.74%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 175 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG+ +F+ G+  N      L + +K    GY IP+GG F LV CP+Y  E IE+ G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 235 SQELYPLCFSTGTAIYLAGRSYATRKWYVSKF-ENFPSHVKALLPF 280
           +     + F   T   L  R+ A+ KWY++KF E +P   KA++PF
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPF 260

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.9e-11
Identity = 47/150 (31.33%), Postives = 72/150 (48.00%), Query Frame = 0


Query: 407 HFFKRIFEVLFVHKYSNKMGLSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLR-AAG 466
           H+FKRI E  FVH++S+       +  +  Y     + I +  V+H L  P  DL+   G
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKIG 200

Query: 467 LAMYWIGITGNLYHHVLLAKLR-KEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 526
                +    N Y H+LL  LR   G   Y+IPRG +F ++ C +Y  E+ ++LG    +
Sbjct: 201 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 260

Query: 527 QTLFPMVFAFGSALFLIGRSYATRNWYVSK 555
           QT+   VF   +AL +        NW + K
Sbjct: 261 QTIAGYVFLAVAALIM-------TNWALGK 282

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.7e-08
Identity = 59/208 (28.37%), Postives = 93/208 (44.71%), Query Frame = 0


Query: 397 IFLINSLLLIHFFKRIFEVLFVHKYSN--KMGLSDAIRVSITYVLLTASTIS--SQMVSH 456
           +FL+  L+ IH  +R+ E  +V KYS   +M +   +     YV    S  S  +  V+ 
Sbjct: 114 VFLL-LLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVAR 173

Query: 457 NLEEPQIDLRAAGL-------------------AMYWIG----ITGNLYH---HVLLAKL 516
            +     +  A+G                    ++ WIG    + G ++    H +L  L
Sbjct: 174 FVGSQVAEFIASGKSHSHDFNLLLSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGSL 233

Query: 517 RK--EGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS-QTLFPMVFAFG-SALFLIG 571
           R+     +EY IP G  FE++ CPH+L E+V +LG+   S  T   +   FG  A  L  
Sbjct: 234 REYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTY 293

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-07
Identity = 40/115 (34.78%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 172 LKNIGIALFLIGIIGNFYHHFLLSKTRK-PGET-GYKIPKGGLFSLVICPHYLFEIIEFF 231
           L+ IG A+FL G I     H +L   R+ P +   Y IP G  F +V  PH+L EI+ + 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 232 GFAFISQ----ELYPLC-FSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           G    S      ++ L  F      Y AG    T +WY+ KFEN+P++  A+ P+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPY 341

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g14270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	6.0e-18	41.90	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	1.3e-17	31.98	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q68FF9	1.3e-17	29.15	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
P24008	1.3e-17	43.27	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
A5PJS2	8.6e-17	31.82	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=...	[more]

BLAST of Cp4.1LG01g14270 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6601243.1	3.46e-313	84.32	3-oxo-5-alpha-steroid 4-dehydrogenase 2, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7032032.1	6.78e-211	56.03	3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023528760.1	4.46e-185	100.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957108.1	5.95e-183	98.86	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_022984951.1	1.20e-182	98.48	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG01g14270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZA3	2.88e-183	98.86	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J6R3	5.80e-183	98.48	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A4D6M1Q7	6.09e-183	51.62	Enoyl reductase OS=Vigna unguiculata OX=3917 GN=DEO72_LG5g2622 PE=3 SV=1	[more]
A0A6J1GYE2	2.26e-172	97.35	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A103Y9N2	7.80e-169	48.42	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. sco...	[more]

BLAST of Cp4.1LG01g14270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	1.3e-84	56.77	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	2.7e-13	37.74	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	1.9e-11	31.33	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	5.7e-08	28.37	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	2.2e-07	34.78	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 106..279 e-value: 2.3E-5 score: 26.2
None	No IPR available	PANTHER	PTHR10556:SF56	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2-LIKE	coord: 310..570
None	No IPR available	PANTHER	PTHR10556:SF56	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2-LIKE	coord: 19..279
None	No IPR available	SUPERFAMILY	101447	Formin homology 2 domain (FH2 domain)	coord: 290..421
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 140..279 e-value: 2.7E-27 score: 95.6 coord: 463..570 e-value: 1.1E-27 score: 96.9
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 465..534 score: 24.230392
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 176..243 score: 25.787687
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 310..570
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 19..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g14270.1	Cp4.1LG01g14270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors