Cp4.1LG01g13540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g13540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationCp4.1LG01: 8158297 .. 8159349 (+)
RNA-Seq ExpressionCp4.1LG01g13540
SyntenyCp4.1LG01g13540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAGAAAAAGAATGCCTATTAACCTAGATCAAATTCCAAGTTTTGAAGAGCATGGTAATGGGTTTGATCAACATTCTGTGTTGGTTGAGGAGATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCTCAGATAGTTCCCAGTGTCTCTAATGCATTGCCACCAGAGCTTGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTACATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCACTTGGAGTTGCTACCATGAGTTCCTGGCCAAGCTATCTGCAAAAGCTGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGACGGATTAAAGACCCTTCAATGGTTGAAACAAGTTGCCATTAATGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTGGAGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCAGCCAGACTTGGTGGCAAGACGACTGCTTCTGGAGCTGTGGTCATGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAGCCGTTCTTCGGTGGAGAGTCAAGGACCATGTCAGAAAAGTTTCTTGTCCAACCTCCTCATTCGCCACTGACGACGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCAGGACGAACCGTGACCATCCATGGTGCAACCCTTCGGCGAATGGAGTGTTTGAGGTTGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGTGGGTAAGCTTATCAATTATGTGGTCTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCTCAAACTCGGACCCTTGAAATGATGGATCATATCAAGGATTTCTTAAGTTTATAG

mRNA sequence

ATGAGGAGAAAAAGAATGCCTATTAACCTAGATCAAATTCCAAGTTTTGAAGAGCATGGTAATGGGTTTGATCAACATTCTGTGTTGGTTGAGGAGATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCTCAGATAGTTCCCAGTGTCTCTAATGCATTGCCACCAGAGCTTGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTACATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCACTTGGAGTTGCTACCATGAGTTCCTGGCCAAGCTATCTGCAAAAGCTGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGACGGATTAAAGACCCTTCAATGGTTGAAACAAGTTGCCATTAATGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTGGAGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCAGCCAGACTTGGTGGCAAGACGACTGCTTCTGGAGCTGTGGTCATGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAGCCGTTCTTCGGTGGAGAGTCAAGGACCATGTCAGAAAAGTTTCTTGTCCAACCTCCTCATTCGCCACTGACGACGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCAGGACGAACCGTGACCATCCATGGTGCAACCCTTCGGCGAATGGAGTGTTTGAGGTTGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGTGGGTAAGCTTATCAATTATGTGGTCTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCTCAAACTCGGACCCTTGAAATGATGGATCATATCAAGGATTTCTTAAGTTTATAG

Coding sequence (CDS)

ATGAGGAGAAAAAGAATGCCTATTAACCTAGATCAAATTCCAAGTTTTGAAGAGCATGGTAATGGGTTTGATCAACATTCTGTGTTGGTTGAGGAGATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGACCTCAGATAGTTCCCAGTGTCTCTAATGCATTGCCACCAGAGCTTGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTACATTCCAACACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGGTTTTGTGTCGGCTCGGCCACTTGGAGTTGCTACCATGAGTTCCTGGCCAAGCTATCTGCAAAAGCTGACTGTATAATTATGTCTGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGACGGATTAAAGACCCTTCAATGGTTGAAACAAGTTGCCATTAATGGGGCCAAGCAGAATTGGTGGTCAAGATACTGCGACTTTACCAAAATCTACCTTGGAGGTGATAGTGCCGGTGGCAACATAGCCTTCAATGTAGCAGCCAGACTTGGTGGCAAGACGACTGCTTCTGGAGCTGTGGTCATGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAGCCGTTCTTCGGTGGAGAGTCAAGGACCATGTCAGAAAAGTTTCTTGTCCAACCTCCTCATTCGCCACTGACGACGGCAGTTTCTGATACGTACTGGCGGTTGGCATTGCCTTCCAGGACGAACCGTGACCATCCATGGTGCAACCCTTCGGCGAATGGAGTGTTTGAGGTTGAGGATTTGAGGGTTTTGCCTAGTTTGATATGTATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCTTTACATAGAGTGGGTAAGCTTATCAATTATGTGGTCTATGAAGGAGTTGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCTCAAACTCGGACCCTTGAAATGATGGATCATATCAAGGATTTCTTAAGTTTATAG

Protein sequence

MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL
Homology
BLAST of Cp4.1LG01g13540 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-92
Identity = 169/332 (50.90%), Postives = 223/332 (67.17%), Query Frame = 0

Query: 21  NGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSWDVVVDKLNNIWAR 80
           N  + H  +V+E+EGLI+VY +G+VER Q++P V  +LP ELGVT  DVV+DKL N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 81  FYIPTQCQE----KLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAP 140
            Y+P    +    KLPL+VYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 141 ENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAARLG 200
           ENPLPA YEDG+  + WL +        N W++ CDF +I+L GDSAGGNIA  VAARL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 201 GKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALP 260
                +       L I+G ILIQPF+ GE RT SE+ +     + LT A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 261 SRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKLINYVV 320
              NR+HP+C P       ++   V  +L+C++EMD+L D N+E C     V   I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVL 314

Query: 321 YEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 349
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Cp4.1LG01g13540 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.7e-85
Identity = 160/335 (47.76%), Postives = 215/335 (64.18%), Query Frame = 0

Query: 20  GNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSWDVVVDKLNNIWA 79
           G+   +H  +VEEIEGLI+V+ +G VERP IVP VS  + P    T++D+ +   N+ W 
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWT 78

Query: 80  RFYIPTQCQEK----LPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLA 139
           R YIP          LPLLVYFHGGGFCVGSA WSCYH+FL  L+ KA C+I+SVNYRLA
Sbjct: 79  RVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLA 138

Query: 140 PENPLPAPYEDGLKTLQWL--KQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAA 199
           PE+ LPA Y+DG+  + WL  +Q++  G   +W S+ C+ + ++L GDSAG NIA+ VA 
Sbjct: 139 PEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAV 198

Query: 200 RLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRL 259
           R+     ASG      L +KG ILI PFFGGESRT SEK       S LT + SD YWRL
Sbjct: 199 RI----MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRL 258

Query: 260 ALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKLIN 319
           ALP   +RDHPWCNP    +      ++  +++ ++E DILK+RNLE C  +   GK + 
Sbjct: 259 ALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVE 318

Query: 320 YVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 349
            +V+ GVGHAF +L+ S +S+ R  +MM  + +F+
Sbjct: 319 GIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Cp4.1LG01g13540 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.1e-50
Identity = 108/328 (32.93%), Postives = 180/328 (54.88%), Query Frame = 0

Query: 29  LVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSW--DVVVDKLNNIWARFYIPTQ 88
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 89  CQEK--LPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 148
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 149 EDGLKTLQWLKQVAINGAKQNWW--SRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTAS 208
           ED    L WL   A++    +W+      DF ++++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 209 GAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRD 268
           G++ + P+ ++G +L+ PFFGGE RT SE     P  + L+  + D +WRL+LP+   RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 269 HPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRV-GKLINYVVYEGVG 328
           H   NP       +E + + P L+ +   ++L+DR  E+   L ++ GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 329 HAFQVLNKSQLSQTRTLEMMDHIKDFLS 350
           H F     S  +  + L ++    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Cp4.1LG01g13540 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 7.3e-43
Identity = 105/301 (34.88%), Postives = 165/301 (54.82%), Query Frame = 0

Query: 45  VERPQIVPSVSNALPPELG--VTSWDVVVDKLNNIWARFYIPTQC---QEKLPLLVYFHG 104
           + RP  +PS + +  P     V + D+ ++ L+N + R ++P        KLPL+VYFHG
Sbjct: 32  ITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHG 91

Query: 105 GGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAI 164
           GGF + SA  + +H+F  +++  A  +I SV+YRLAPE+ LPA Y+D ++ LQW+K    
Sbjct: 92  GGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD--- 151

Query: 165 NGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQ 224
             ++  W + + DF+  ++ G+SAGGNIA++   R      A+ A  + PL IKG +L +
Sbjct: 152 --SRDEWLTNFADFSNCFIMGESAGGNIAYHAGLR-----AAAVADELLPLKIKGLVLDE 211

Query: 225 PFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRDHPWCNPSANG--VFEVE 284
           P FGG  RT SE  L     S L T V D  W L+LP   +RDH +CNP+A    ++  +
Sbjct: 212 PGFGGSKRTGSELRLAN--DSRLPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFD 271

Query: 285 DLRVLPSLICI--SEMDILKDRNLEFCSALHRVGKLINYVVYEGVG--HAFQVLNKSQLS 335
            +R L   + +     D + DR +E    L + G  ++ V    VG  HA ++ +  +  
Sbjct: 272 KIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKG--VDVVAQFDVGGYHAVKLEDPEKAK 318

BLAST of Cp4.1LG01g13540 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.4e-41
Identity = 106/307 (34.53%), Postives = 155/307 (50.49%), Query Frame = 0

Query: 32  EIEGLIRVYGNGYVER---PQIVPSVSNALPPELGVTSWDVVVDKLNNIWARFYIP--TQ 91
           E+  L+RVY +G VER      VP   +   PE GV++ D+V+ +   I AR Y+P    
Sbjct: 16  ELPPLLRVYKDGTVERFLGSSFVP--PSPEDPETGVSTKDIVISENPTISARVYLPKLNN 75

Query: 92  CQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYED 151
             EKLP+LVY+HGG FC+ SA    +  +L  +++KA+ +++S+ YRLAPE+PLPA YED
Sbjct: 76  TTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYED 135

Query: 152 GLKTLQWLKQVAINGAKQN----WWSRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTAS 211
           G   L+W+   + N  K      W  ++ DF + Y+GGD++G NIA N A R+G +    
Sbjct: 136 GWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEALPG 195

Query: 212 GAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNR- 271
           G      L I G +   P F G    +SE        SP+        W    P      
Sbjct: 196 G------LRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQV------WNFVYPDAPGGI 255

Query: 272 DHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKL--INYVVYEG 327
           D+P  NP A G   +  L     L+ ++  D L+DR + +  A+   G    +    YEG
Sbjct: 256 DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEG 308

BLAST of Cp4.1LG01g13540 vs. NCBI nr
Match: XP_023528052.1 (probable carboxylesterase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 725 bits (1871), Expect = 4.31e-264
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60
           MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP
Sbjct: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60

Query: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120
           ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS
Sbjct: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120

Query: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180
           AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG
Sbjct: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180

Query: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240
           DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS
Sbjct: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240

Query: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300
           PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE
Sbjct: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300

Query: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL
Sbjct: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350

BLAST of Cp4.1LG01g13540 vs. NCBI nr
Match: XP_022957509.1 (probable carboxylesterase 6 [Cucurbita moschata])

HSP 1 Score: 720 bits (1859), Expect = 2.91e-262
Identity = 347/350 (99.14%), Postives = 348/350 (99.43%), Query Frame = 0

Query: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60
           MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP
Sbjct: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60

Query: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120
           ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS
Sbjct: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120

Query: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180
           AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA NGAKQNWWSRYCDFTKIYLGG
Sbjct: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLGG 180

Query: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240
           DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS
Sbjct: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240

Query: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300
           PLTTAVSDTYWRLALPSR NRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE
Sbjct: 241 PLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300

Query: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLS+
Sbjct: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cp4.1LG01g13540 vs. NCBI nr
Match: XP_022982294.1 (probable carboxylesterase 6 [Cucurbita maxima] >XP_022982375.1 probable carboxylesterase 6 [Cucurbita maxima] >XP_022982445.1 probable carboxylesterase 6 [Cucurbita maxima])

HSP 1 Score: 714 bits (1844), Expect = 5.63e-260
Identity = 344/350 (98.29%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60
           MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP
Sbjct: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60

Query: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120
           ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSA+WSCYHEFLAKLS
Sbjct: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKLS 120

Query: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180
           AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA NGAKQNWWSRYCDFTKIYLGG
Sbjct: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLGG 180

Query: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240
           DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRT SEKFLVQPPHS
Sbjct: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPHS 240

Query: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300
           PLTTAVSDTYWRLALPSR NRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDR+LE
Sbjct: 241 PLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSLE 300

Query: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLS+
Sbjct: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cp4.1LG01g13540 vs. NCBI nr
Match: KAG6601310.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032094.1 putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 711 bits (1834), Expect = 1.56e-258
Identity = 342/345 (99.13%), Postives = 343/345 (99.42%), Query Frame = 0

Query: 6   MPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVT 65
           MPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVT
Sbjct: 1   MPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVT 60

Query: 66  SWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADC 125
           SWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADC
Sbjct: 61  SWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADC 120

Query: 126 IIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGGDSAGG 185
           IIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA NGAKQNWWSRYCDFTKIYLGGDSAGG
Sbjct: 121 IIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLGGDSAGG 180

Query: 186 NIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTA 245
           NIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTA
Sbjct: 181 NIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTA 240

Query: 246 VSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSAL 305
           VSDTYWRLALPSR NRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSAL
Sbjct: 241 VSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSAL 300

Query: 306 HRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           HRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLS+
Sbjct: 301 HRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 345

BLAST of Cp4.1LG01g13540 vs. NCBI nr
Match: XP_038893358.1 (probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893360.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893361.1 probable carboxylesterase 6 [Benincasa hispida])

HSP 1 Score: 679 bits (1751), Expect = 8.80e-246
Identity = 328/349 (93.98%), Postives = 335/349 (95.99%), Query Frame = 0

Query: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60
           MR KRMP INLDQIPSFEEHGNGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKL 120
           PELGVTSWDVV+DKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVMDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLG 180
           SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA  GAKQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPH 240
           GDSAGGNIAFNVAARLGGKTTASGAVV+KPLVIKG+ILIQPFFGGESRT SEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVLKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNL 300
           SPLT AVSDTYWRLALPS TNRDHPWCNPSA G+F+VEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIKDFL
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 349

BLAST of Cp4.1LG01g13540 vs. ExPASy TrEMBL
Match: A0A6J1H254 (probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111458883 PE=4 SV=1)

HSP 1 Score: 720 bits (1859), Expect = 1.41e-262
Identity = 347/350 (99.14%), Postives = 348/350 (99.43%), Query Frame = 0

Query: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60
           MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP
Sbjct: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60

Query: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120
           ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS
Sbjct: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120

Query: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180
           AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA NGAKQNWWSRYCDFTKIYLGG
Sbjct: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLGG 180

Query: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240
           DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS
Sbjct: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240

Query: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300
           PLTTAVSDTYWRLALPSR NRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE
Sbjct: 241 PLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300

Query: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLS+
Sbjct: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cp4.1LG01g13540 vs. ExPASy TrEMBL
Match: A0A6J1J448 (probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111481140 PE=4 SV=1)

HSP 1 Score: 714 bits (1844), Expect = 2.73e-260
Identity = 344/350 (98.29%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60
           MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP
Sbjct: 1   MRRKRMPINLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPP 60

Query: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLS 120
           ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSA+WSCYHEFLAKLS
Sbjct: 61  ELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKLS 120

Query: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGG 180
           AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA NGAKQNWWSRYCDFTKIYLGG
Sbjct: 121 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLGG 180

Query: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHS 240
           DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRT SEKFLVQPPHS
Sbjct: 181 DSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPHS 240

Query: 241 PLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLE 300
           PLTTAVSDTYWRLALPSR NRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDR+LE
Sbjct: 241 PLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSLE 300

Query: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSL 350
           FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLS+
Sbjct: 301 FCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cp4.1LG01g13540 vs. ExPASy TrEMBL
Match: A0A0A0KUG5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608500 PE=4 SV=1)

HSP 1 Score: 665 bits (1717), Expect = 6.48e-241
Identity = 322/349 (92.26%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60
           MR KRMP INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLG 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA  G KQNWWSRYCDFTKIYL 
Sbjct: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPH 240
           GDSAGGNIAFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRT SEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNL 300
           SPLT  VSDTYWRLALPS TNRDHPWCNPS  G+F VEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIK FL
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFL 349

BLAST of Cp4.1LG01g13540 vs. ExPASy TrEMBL
Match: A0A5A7SV76 (Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00820 PE=4 SV=1)

HSP 1 Score: 664 bits (1712), Expect = 3.75e-240
Identity = 320/349 (91.69%), Postives = 328/349 (93.98%), Query Frame = 0

Query: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60
           MR K+MP INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRTKKMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLG 180
           SAK+DCII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVA  G KQNWWSRYCDFTKIYL 
Sbjct: 121 SAKSDCIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPH 240
           GDSAGGNIAFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRT SEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPR 240

Query: 241 SPLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNL 300
           SPLT  VSDTYWRLALPS TNRDHPWCNPS  G+F VED RVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFL
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFL 349

BLAST of Cp4.1LG01g13540 vs. ExPASy TrEMBL
Match: A0A1S3BFC8 (probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1)

HSP 1 Score: 657 bits (1696), Expect = 8.53e-238
Identity = 315/341 (92.38%), Postives = 322/341 (94.43%), Query Frame = 0

Query: 8   INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSW 67
           INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALPPELGVTSW
Sbjct: 4   INLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGVTSW 63

Query: 68  DVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCII 127
           DVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKLSAK+DCII
Sbjct: 64  DVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSDCII 123

Query: 128 MSVNYRLAPENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNI 187
           +SVNYRLAPENPLPAPYEDGLKTLQWLKQVA  G KQNWWSRYCDFTKIYL GDSAGGNI
Sbjct: 124 ISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAGGNI 183

Query: 188 AFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVS 247
           AFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRT SEKFLVQPP SPLT  VS
Sbjct: 184 AFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTLGVS 243

Query: 248 DTYWRLALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHR 307
           DTYWRLALPS TNRDHPWCNPS  G+F VED RVLPSLICISEMDILKDRNLEFCSALHR
Sbjct: 244 DTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSALHR 303

Query: 308 VGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 348
            GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFL
Sbjct: 304 AGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFL 344

BLAST of Cp4.1LG01g13540 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-93
Identity = 169/332 (50.90%), Postives = 223/332 (67.17%), Query Frame = 0

Query: 21  NGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSWDVVVDKLNNIWAR 80
           N  + H  +V+E+EGLI+VY +G+VER Q++P V  +LP ELGVT  DVV+DKL N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 81  FYIPTQCQE----KLPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAP 140
            Y+P    +    KLPL+VYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 141 ENPLPAPYEDGLKTLQWLKQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAARLG 200
           ENPLPA YEDG+  + WL +        N W++ CDF +I+L GDSAGGNIA  VAARL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 201 GKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALP 260
                +       L I+G ILIQPF+ GE RT SE+ +     + LT A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 261 SRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKLINYVV 320
              NR+HP+C P       ++   V  +L+C++EMD+L D N+E C     V   I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVL 314

Query: 321 YEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 349
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Cp4.1LG01g13540 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 315.5 bits (807), Expect = 5.5e-86
Identity = 160/335 (47.76%), Postives = 215/335 (64.18%), Query Frame = 0

Query: 20  GNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSWDVVVDKLNNIWA 79
           G+   +H  +VEEIEGLI+V+ +G VERP IVP VS  + P    T++D+ +   N+ W 
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWT 78

Query: 80  RFYIPTQCQEK----LPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLA 139
           R YIP          LPLLVYFHGGGFCVGSA WSCYH+FL  L+ KA C+I+SVNYRLA
Sbjct: 79  RVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLA 138

Query: 140 PENPLPAPYEDGLKTLQWL--KQVAINGAKQNWWSRYCDFTKIYLGGDSAGGNIAFNVAA 199
           PE+ LPA Y+DG+  + WL  +Q++  G   +W S+ C+ + ++L GDSAG NIA+ VA 
Sbjct: 139 PEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAV 198

Query: 200 RLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRL 259
           R+     ASG      L +KG ILI PFFGGESRT SEK       S LT + SD YWRL
Sbjct: 199 RI----MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRL 258

Query: 260 ALPSRTNRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRVGKLIN 319
           ALP   +RDHPWCNP    +      ++  +++ ++E DILK+RNLE C  +   GK + 
Sbjct: 259 ALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVE 318

Query: 320 YVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFL 349
            +V+ GVGHAF +L+ S +S+ R  +MM  + +F+
Sbjct: 319 GIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Cp4.1LG01g13540 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-51
Identity = 108/328 (32.93%), Postives = 180/328 (54.88%), Query Frame = 0

Query: 29  LVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSW--DVVVDKLNNIWARFYIPTQ 88
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 89  CQEK--LPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 148
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 149 EDGLKTLQWLKQVAINGAKQNWW--SRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTAS 208
           ED    L WL   A++    +W+      DF ++++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 209 GAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRD 268
           G++ + P+ ++G +L+ PFFGGE RT SE     P  + L+  + D +WRL+LP+   RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 269 HPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRV-GKLINYVVYEGVG 328
           H   NP       +E + + P L+ +   ++L+DR  E+   L ++ GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 329 HAFQVLNKSQLSQTRTLEMMDHIKDFLS 350
           H F     S  +  + L ++    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Cp4.1LG01g13540 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-51
Identity = 108/328 (32.93%), Postives = 180/328 (54.88%), Query Frame = 0

Query: 29  LVEEIEGLIRVYGNGYVERPQIVPSVSNALPPELGVTSW--DVVVDKLNNIWARFYIPTQ 88
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 89  CQEK--LPLLVYFHGGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 148
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 149 EDGLKTLQWLKQVAINGAKQNWW--SRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTAS 208
           ED    L WL   A++    +W+      DF ++++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 209 GAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRD 268
           G++ + P+ ++G +L+ PFFGGE RT SE     P  + L+  + D +WRL+LP+   RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 269 HPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNLEFCSALHRV-GKLINYVVYEGVG 328
           H   NP       +E + + P L+ +   ++L+DR  E+   L ++ GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 329 HAFQVLNKSQLSQTRTLEMMDHIKDFLS 350
           H F     S  +  + L ++    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Cp4.1LG01g13540 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 1.1e-38
Identity = 101/312 (32.37%), Postives = 154/312 (49.36%), Query Frame = 0

Query: 55  SNALPPELGVTSWDVVVDKLNNIWARFYIPTQCQEK----------------LPLLVYFH 114
           +NA P + GV S+DV++D+  N+ +R Y P    ++                +P++++FH
Sbjct: 55  ANANPVD-GVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFH 114

Query: 115 GGGFCVGSATWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVA 174
           GG F   SA  + Y     +L     C+++SVNYR APENP P  Y+DG   L W+    
Sbjct: 115 GGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVN--- 174

Query: 175 INGAKQNWW-SRYCDFTKIYLGGDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNIL 234
                ++W  S+      I+L GDS+GGNIA NVA R G     SG  V+      GNIL
Sbjct: 175 ----SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAG----ESGIDVL------GNIL 234

Query: 235 IQPFFGGESRTMSEKFLVQPPHSPLTTAVSDTYWRLALPSRTNRDHPWCNPSANGVFEVE 294
           + P FGG  RT SEK L       +T    D YW+  LP   +R+HP CNP +     +E
Sbjct: 235 LNPMFGGNERTESEKSL--DGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLE 294

Query: 295 DLRVLPSLICISEMDILKDRNLEFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRT 350
            +    SL+ ++ +D+++D  L +   L + G+ +  +  E     F +L     +    
Sbjct: 295 GVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLP----NNNHF 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX251.7e-9250.90Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR77.7e-8547.76Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9FG132.1e-5032.93Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV77.3e-4334.88Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q5NUF42.4e-4134.532-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023528052.14.31e-264100.00probable carboxylesterase 6 [Cucurbita pepo subsp. pepo][more]
XP_022957509.12.91e-26299.14probable carboxylesterase 6 [Cucurbita moschata][more]
XP_022982294.15.63e-26098.29probable carboxylesterase 6 [Cucurbita maxima] >XP_022982375.1 probable carboxyl... [more]
KAG6601310.11.56e-25899.13putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_038893358.18.80e-24693.98probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxy... [more]
Match NameE-valueIdentityDescription
A0A6J1H2541.41e-26299.14probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111458883 PE=4 S... [more]
A0A6J1J4482.73e-26098.29probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111481140 PE=4 SV=... [more]
A0A0A0KUG56.48e-24192.26Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6085... [more]
A0A5A7SV763.75e-24091.69Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BFC88.53e-23892.38probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68620.11.2e-9350.90alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.15.5e-8647.76carboxyesterase 17 [more]
AT5G06570.11.5e-5132.93alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.5e-5132.93alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.11.1e-3832.37alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 94..325
e-value: 3.3E-54
score: 184.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 46..350
e-value: 2.3E-71
score: 242.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 28..348
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 20..348
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 20..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g13540.1Cp4.1LG01g13540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity