Cp4.1LG01g13070 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g13070 vs. ExPASy Swiss-Prot
Match: Q944S1 (DEAD-box ATP-dependent RNA helicase 22 OS=Arabidopsis thaliana OX=3702 GN=RH22 PE=2 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.1e-168
Identity = 335/581 (57.66%), Postives = 414/581 (71.26%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASS--SKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGT 60
           M+L RS S L+L  ++SS  SKLL      +  +    S+   L  L +   K  R    
Sbjct: 1   MILSRSVSVLHLCGVSSSAPSKLLSQRFKVSFALAYGSSVSFRLSSLNRSDRKWVRGF-- 60

Query: 61  VAASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMS 120
             ASA     +  G DTF+A   VSW SLG+SDNVS AL  +G  RPSL QA C+PSI+S
Sbjct: 61  --ASATEAEVEKKGNDTFFADHTVSWKSLGLSDNVSIALRDSGFDRPSLTQAVCIPSILS 120

Query: 121 GNDVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCE 180
           G DV++AAETGSGKTHGYL P+I+++ N   D ++T+ ++    +  +SL+LCPNVMLCE
Sbjct: 121 GKDVIVAAETGSGKTHGYLAPIIDQLTNTALDSEVTNREERPFPLKNISLILCPNVMLCE 180

Query: 181 QVVQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLC 240
           QVV+M N L DE G  LLRV  VCG QGWP   PDIIVSTPAALLN IEP R+RR++FL 
Sbjct: 181 QVVRMVNGLVDEDGNPLLRVEAVCGSQGWPDRLPDIIVSTPAALLNNIEPKRNRRLEFLR 240

Query: 241 AVKHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSR-SKESPERLMELEAD-PSSQLT 300
            VK+VVFDEADMLL GSFQN++IRLIN+LRF+EK +SR +K +  R ME++A  P   L 
Sbjct: 241 CVKYVVFDEADMLLCGSFQNQIIRLINMLRFDEKQVSRLAKSNLGRPMEIDASVPQIDLE 300

Query: 301 LQDESDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQ 360
            +D+++ +  +ISE E E E EE  D+     +++   E  S   K WRR +K Y RSKQ
Sbjct: 301 NEDDAEFDEGSISEEEDEEEEEEYLDDIAQMPSVE--AEAGSDTKKGWRRVRKIYTRSKQ 360

Query: 361 YIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIK 420
           YIF+AATLPVNGKKTAG +L+ MF DA WVSG +LH +SPRL+Q+WVEVTVD+QVD LI+
Sbjct: 361 YIFIAATLPVNGKKTAGGILKHMFQDAVWVSGNFLHRNSPRLKQKWVEVTVDSQVDALIE 420

Query: 421 AVNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILA 480
           AV        N + +  RTMVFANTVE VEAVA+IL  A  +C+RYHK+  L+ER+ ILA
Sbjct: 421 AVK------NNNNTNTERTMVFANTVEAVEAVADILEKASIQCYRYHKNHKLDERANILA 480

Query: 481 DFRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYT 540
           DFRE GGV VCTDAAARGVD+PNV+HVIQADFA+SAVDFLHRIGRT RAGQ G VTS+YT
Sbjct: 481 DFRETGGVFVCTDAAARGVDVPNVSHVIQADFASSAVDFLHRIGRTARAGQYGTVTSLYT 540

Query: 541 KANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAK 578
           +ANRDLV A+R A  + QPVE AFSRKR FRNK+KK+   K
Sbjct: 541 EANRDLVEAIREAVKMGQPVETAFSRKRGFRNKVKKRAFLK 569

BLAST of Cp4.1LG01g13070 vs. ExPASy Swiss-Prot
Match: Q6H601 (DEAD-box ATP-dependent RNA helicase 22 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0383400 PE=2 SV=2)

HSP 1 Score: 555.1 bits (1429), Expect = 9.7e-157
Identity = 302/536 (56.34%), Postives = 386/536 (72.01%), Query Frame = 0

Query: 54  RAVGTVAASAATPVADGIG-RDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAAC 113
           RA+ T AA A    A G G  D F++ E  SW SLGVSD ++ AL  AG+ RPSL+QAAC
Sbjct: 53  RALATAAAEADDAGAGGNGDGDGFFSEESTSWESLGVSDRLASALHGAGLARPSLVQAAC 112

Query: 114 VPSIMSGNDVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGD-QDLASIN-KLSLVL 173
           +P +++ NDV++AAETGSGKTHGYLVPLI K+C+  +     DG+ QD+ S +  ++LVL
Sbjct: 113 IPHVLTTNDVIVAAETGSGKTHGYLVPLIEKLCS--KSISAEDGNSQDVTSGSPNIALVL 172

Query: 174 CPNVMLCEQVVQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYI---E 233
           CPNVMLCEQVV+MAN+L DE G+ L     VCG +GWP  +PDI+V+TPAALLNY+   +
Sbjct: 173 CPNVMLCEQVVRMANSLVDESGEPLKSAAAVCGPKGWPTVRPDILVATPAALLNYLFDYD 232

Query: 234 PNRSRRMKFLCAVKHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMEL 293
           P + RR +FL  VK +VFDEADMLL GSF+N+VIRLI++LRF+EKLLSR ++S       
Sbjct: 233 PEKRRRERFLRNVKFIVFDEADMLLCGSFENQVIRLIHMLRFDEKLLSRMEDS------- 292

Query: 294 EADPSSQLTLQDESDIETETISE-GEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRR 353
                 +++L D ++   ++ S+  E   + EE+ D  V    +  +  +     KDWRR
Sbjct: 293 ----GKEISLGDTNEYREDSDSQSAELSADDEENEDGLVQHRPVNVENAHIGAHKKDWRR 352

Query: 354 AQKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVT 413
            +K Y RSKQY+FVAATLP +GKKTAG VL++MFP+A WVSG YLH H+PRLE+RW+EVT
Sbjct: 353 VRKVYRRSKQYVFVAATLPQSGKKTAGGVLKRMFPNAVWVSGAYLHRHNPRLERRWIEVT 412

Query: 414 VDNQVDELIKAVNQS-KSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKD 473
            D QV  L+ AV    K+++ +      RTMVF NTV+   +V++IL   G  C  YH+D
Sbjct: 413 ADTQVSALLDAVKYGLKNEVHDTKLGPNRTMVFTNTVDAANSVSDILQRVGVPCILYHRD 472

Query: 474 RSLEERSKILADFRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRA 533
            SLEER+K L  FRENGGVLVCTDAAARG+D+PNV+HVIQA+FA  AVDFLHR+GRT RA
Sbjct: 473 SSLEERAKNLQSFRENGGVLVCTDAAARGLDVPNVSHVIQAEFAACAVDFLHRVGRTARA 532

Query: 534 GQCGLVTSMYTKANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVL 582
           GQ G+VTS+YT+ANRDLV AVR+AE L+QPVE AFSRKRSFRNKLKKQ   K + L
Sbjct: 533 GQSGIVTSLYTEANRDLVRAVRQAEELAQPVEKAFSRKRSFRNKLKKQALHKSTAL 575

BLAST of Cp4.1LG01g13070 vs. ExPASy Swiss-Prot
Match: Q53RK8 (DEAD-box ATP-dependent RNA helicase 21 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0708600 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.2e-28
Identity = 127/466 (27.25%), Postives = 204/466 (43.78%), Query Frame = 0

Query: 84  WTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLINK 143
           W+   +   + RA+  AG + PS IQ A +P  +   DV+  AETGSGKT  +++P+++ 
Sbjct: 317 WSESKLGTELLRAVEKAGYKEPSPIQMASIPLGLQQRDVIGIAETGSGKTAAFVLPMLSY 376

Query: 144 ICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVCG 203
           I       +L    ++  +    ++V+ P   L +Q+ +         G   ++V ++ G
Sbjct: 377 I------TRLPPISEENEAEGPYAVVMAPTRELAQQIEEETVKFATYLG---IKVVSIVG 436

Query: 204 -----RQGWPVYKP-DIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVGSFQ 263
                 QG+ + +  +++++TP  LL+ +E    RR   L    +VV DEAD ++   F+
Sbjct: 437 GQSIEEQGFKIRQGCEVVIATPGRLLDCLE----RRYAVLNQCNYVVLDEADRMIDMGFE 496

Query: 264 NKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEGEGEG 323
            +V+ +                       L+A PSS L  ++E                 
Sbjct: 497 PQVVGV-----------------------LDAMPSSNLKPENE----------------- 556

Query: 324 EESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVLR 383
               DE++D+  I   T                      Y+F +AT+P   ++ A   LR
Sbjct: 557 ----DEELDAKTIYRTT----------------------YMF-SATMPPAVERLARKYLR 616

Query: 384 KMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTMV 443
                    +GK     +  L  + V +T +++          S+ Q    D      +V
Sbjct: 617 NPVVVTIGTAGK-----ATDLITQNVIMTKESE--------KMSRLQKILTDLGDKPAIV 676

Query: 444 FANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRENG-GVLVCTDAAARGVD 503
           F NT ++ +A A  L  AG      H  +S E+R   L  FR     VLV TD A RG+D
Sbjct: 677 FCNTKKSADARAKDLDKAGFRVTTLHGGKSQEQRETSLDGFRNRRFTVLVATDVAGRGID 689

Query: 504 IPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANRDL 543
           IP+VAHVI  +  +S   + HRIGRTGRAG+ GL TS  T  N D+
Sbjct: 737 IPDVAHVINYEMPSSIDTYTHRIGRTGRAGKKGLATSFLTLENTDI 689

BLAST of Cp4.1LG01g13070 vs. ExPASy Swiss-Prot
Match: Q5VRY0 (DEAD-box ATP-dependent RNA helicase 39 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0184500 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.5e-27
Identity = 125/491 (25.46%), Postives = 205/491 (41.75%), Query Frame = 0

Query: 83  SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLIN 142
           S+  LG+ + V  AL   GI +P+ IQ   VP++++G  VV+ + TGSGKT  YL+PL+ 
Sbjct: 111 SFEELGLGEEVMAALGEMGISKPTEIQCVGVPAVLAGTSVVLGSHTGSGKTLAYLLPLVQ 170

Query: 143 KICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVC 202
            +   +RD+ +      +      ++VLCP   L EQV ++A ++   H +   R T V 
Sbjct: 171 LL---RRDEAML--GMSMKPRRPRAVVLCPTRELTEQVFRVAKSI-SHHAR--FRSTMVS 230

Query: 203 GRQ---------GWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLV 262
           G             PV   D++V TP  +L++I+            +K++V DEAD +  
Sbjct: 231 GGSRIRPQEDSLNMPV---DMVVGTPGRILDHIKDGN----MVYGDIKYLVLDEADTMFD 290

Query: 263 GSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEG 322
             F   + + +  L+                                             
Sbjct: 291 QGFGPDIRKFLAPLK--------------------------------------------- 350

Query: 323 EGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAG 382
                                           RA K  ++  Q + V AT+     K   
Sbjct: 351 -------------------------------NRAAKPGDQGFQTVLVTATM----TKAVQ 410

Query: 383 AVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAI 442
            ++ + F          +H  +   ++R    T  +   +L  + N+ ++ L+ ++ S  
Sbjct: 411 KLIDEEFEG-------IVHLRTTTFQKR--VATARHDFIKLSGSENKLEALLQVLEPSLA 470

Query: 443 ---RTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRENGG---VLVC 502
              + MVF NT+ +  AV + L+        YH +   EER + L  FR   G    LVC
Sbjct: 471 KGNKVMVFCNTLNSSRAVDHFLTENQISTVNYHGEVPAEERVENLNKFRNEEGDCPTLVC 496

Query: 503 TDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANRDLVAAVR 559
           TD AARG+D+ +V HVI  DF ++++D+LHR GRT R G  G VTS+  K +  L   + 
Sbjct: 531 TDLAARGLDL-DVDHVIMFDFPSNSIDYLHRTGRTARMGAKGKVTSLVAKKDVTLATRIE 496

BLAST of Cp4.1LG01g13070 vs. ExPASy Swiss-Prot
Match: Q9Y7T7 (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prp28 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.2e-27
Identity = 122/472 (25.85%), Postives = 195/472 (41.31%), Query Frame = 0

Query: 83  SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLIN 142
           +W   G+   + + L     + PS IQ A +P ++   D++  AETGSGKT  +++PLI 
Sbjct: 250 NWEEAGLPSEMLKVLKKVNYKEPSSIQRAAIPVLLQRKDLIGIAETGSGKTAAFIIPLII 309

Query: 143 KICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVC 202
            I       KL    +    +   ++VL P   L +Q+    N   +  G    R  +V 
Sbjct: 310 AI------SKLPPLTESNMHLGPYAVVLAPTRELAQQIQVEGNKFAEPLG---FRCVSVV 369

Query: 203 GRQGWPVYK------PDIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVGSF 262
           G   +            I+V+TP  LL+ +E    RR+  L    +VV DEAD +L   F
Sbjct: 370 GGHAFEEQSFQMSQGAHIVVATPGRLLDCLE----RRLFVLSQCTYVVMDEADRMLDMGF 429

Query: 263 QNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEGEGE 322
           ++ V ++++ L                       PSS  + +D S + T   S       
Sbjct: 430 EDDVNKILSSL-----------------------PSSNASEKDGSILATANSS------- 489

Query: 323 GEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVL 382
                               SSRR               Q I  +ATLP      A + L
Sbjct: 490 --------------------SSRR---------------QTIMFSATLPPRVANLAKSYL 549

Query: 383 RKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTM 442
            +         G+ +     R+EQR   ++ D++    ++ + +S      I       +
Sbjct: 550 IEPVMLTIGNIGQAV----DRVEQRVEMISDDSKKWRRVEEILESNRFSPPI-------I 609

Query: 443 VFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFR-ENGGVLVCTDAAARGV 502
           +F N    +EA+A  L+  G      H  +S E+R + +   R +   +LV TD A RG+
Sbjct: 610 IFVNLKRNIEAIAKQLNAIGWHAVTLHGSKSQEQRERAIEQLRNKTADILVATDIAGRGI 632

Query: 503 DIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANRDLVAAVR 548
           DIPNV+ V+  + A S  D+ HRIGRTGRAG+ G   +     + D+   +R
Sbjct: 670 DIPNVSLVLNYNMAKSIEDYTHRIGRTGRAGKSGTAITFLGPEDTDVYYDLR 632

BLAST of Cp4.1LG01g13070 vs. NCBI nr
Match: XP_023549678.1 (DEAD-box ATP-dependent RNA helicase 22 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1115 bits (2885), Expect = 0.0
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
           NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS
Sbjct: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582

BLAST of Cp4.1LG01g13070 vs. NCBI nr
Match: KAG6601380.1 (DEAD-box ATP-dependent RNA helicase 22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 573/582 (98.45%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLR+PCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRKPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRD     GDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRD-----GDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEAD SSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADASSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SKSQLRNIDG AIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKSQLRNIDGGAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
           NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS
Sbjct: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 577

BLAST of Cp4.1LG01g13070 vs. NCBI nr
Match: XP_022956592.1 (DEAD-box ATP-dependent RNA helicase 22 [Cucurbita moschata])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 572/582 (98.28%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLR+PCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRKPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATPVADGIGRDTFYAAEG+SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPVADGIGRDTFYAAEGISWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRD     GDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRD-----GDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEAD SSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADASSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SKSQLRNIDG AIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKSQLRNIDGGAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
           NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS
Sbjct: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 577

BLAST of Cp4.1LG01g13070 vs. NCBI nr
Match: XP_022997352.1 (DEAD-box ATP-dependent RNA helicase 22 [Cucurbita maxima])

HSP 1 Score: 1085 bits (2807), Expect = 0.0
Identity = 567/582 (97.42%), Postives = 572/582 (98.28%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATP ADGIGRDTFYAAEGVSWTSLGVSDNVSR+LVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPAADGIGRDTFYAAEGVSWTSLGVSDNVSRSLVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESY EDVDSNNIQ+KTEYSS RVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESY-EDVDSNNIQDKTEYSSGRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQ DELI+AVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQADELIEAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SK+QLRNID  AIRTMVFANTVETVEAVANIL VAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKAQLRNIDDGAIRTMVFANTVETVEAVANILLVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
            GGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 KGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAA+RRAENLSQPVEAAFSRKRSFRNKLKKQGS KESVLS
Sbjct: 541 DLVAAIRRAENLSQPVEAAFSRKRSFRNKLKKQGSGKESVLS 581

BLAST of Cp4.1LG01g13070 vs. NCBI nr
Match: XP_038892424.1 (DEAD-box ATP-dependent RNA helicase 22 [Benincasa hispida])

HSP 1 Score: 989 bits (2557), Expect = 0.0
Identity = 515/585 (88.03%), Postives = 549/585 (93.85%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSAS L+LERLA SSKLLFS KN NLLVP+SCSIRIGLVCLRQPCS+QSRAVGTVA
Sbjct: 1   MLLYRSASVLHLERLALSSKLLFSLKNYNLLVPNSCSIRIGLVCLRQPCSRQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAA+  ADGIG+DTFYAAEGVSWTSLGVSDNVSRALVSAG+QRPSLIQAACVPSIMSGN
Sbjct: 61  ASAASTAADGIGKDTFYAAEGVSWTSLGVSDNVSRALVSAGMQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKIC+E  +DKLTDGDQDLAS NKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICSEHGEDKLTDGDQDLASFNKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMAN+LCDEHGKSLLRVT VCGRQGWP++KPDI+VSTPAALLNYIEPNR RR +FL AV
Sbjct: 181 VQMANSLCDEHGKSLLRVTAVCGRQGWPIHKPDIVVSTPAALLNYIEPNRIRRSEFLRAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRS+ESPE LMELEA P SQL +QDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSRESPEGLMELEAGPLSQLIVQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDE---DVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQY 360
           +D++TETI EGEGEGEGEE  +E   +VDSNNIQE+TE  S R+ DWRR +KSY+RSKQY
Sbjct: 301 NDLQTETIPEGEGEGEGEEDGEESCTEVDSNNIQEETECVSGRINDWRRVRKSYKRSKQY 360

Query: 361 IFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKA 420
           IFVAATLPVNGKKTAGAVLRKM+PDASWVSGKYLHCH+PRLEQRWVEVT+DNQVDELIKA
Sbjct: 361 IFVAATLPVNGKKTAGAVLRKMYPDASWVSGKYLHCHNPRLEQRWVEVTLDNQVDELIKA 420

Query: 421 VNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILAD 480
           VNQSKSQLRN DGSA+RTMVFANTVETVEAVANIL  AG ECFRYHKDRSLEERSKILAD
Sbjct: 421 VNQSKSQLRNSDGSAVRTMVFANTVETVEAVANILQGAGKECFRYHKDRSLEERSKILAD 480

Query: 481 FRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTK 540
           FRE GGV VCTDAAARGVDIPNV+HVIQADFATSAVDFLHRIGRTGRAGQ GLVTS+YTK
Sbjct: 481 FREKGGVFVCTDAAARGVDIPNVSHVIQADFATSAVDFLHRIGRTGRAGQYGLVTSLYTK 540

Query: 541 ANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           AN DLV+AVRRAENLSQPVEAAFSRKRSFRNKLKKQGS+KESV++
Sbjct: 541 ANCDLVSAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSSKESVMA 585

BLAST of Cp4.1LG01g13070 vs. ExPASy TrEMBL
Match: A0A6J1GWS3 (DEAD-box ATP-dependent RNA helicase 22 OS=Cucurbita moschata OX=3662 GN=LOC111458282 PE=4 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 572/582 (98.28%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLR+PCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRKPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATPVADGIGRDTFYAAEG+SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPVADGIGRDTFYAAEGISWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRD     GDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRD-----GDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEAD SSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADASSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SKSQLRNIDG AIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKSQLRNIDGGAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
           NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS
Sbjct: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 577

BLAST of Cp4.1LG01g13070 vs. ExPASy TrEMBL
Match: A0A6J1K4R5 (DEAD-box ATP-dependent RNA helicase 22 OS=Cucurbita maxima OX=3661 GN=LOC111492263 PE=4 SV=1)

HSP 1 Score: 1085 bits (2807), Expect = 0.0
Identity = 567/582 (97.42%), Postives = 572/582 (98.28%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA
Sbjct: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAATP ADGIGRDTFYAAEGVSWTSLGVSDNVSR+LVSAGIQRPSLIQAACVPSIMSGN
Sbjct: 61  ASAATPAADGIGRDTFYAAEGVSWTSLGVSDNVSRSLVSAGIQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           SDIETETISEGEGEGEGEESY EDVDSNNIQ+KTEYSS RVKDWRRAQKSYERSKQYIFV
Sbjct: 301 SDIETETISEGEGEGEGEESY-EDVDSNNIQDKTEYSSGRVKDWRRAQKSYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQ DELI+AVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQADELIEAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
           SK+QLRNID  AIRTMVFANTVETVEAVANIL VAGTECFRYHKDRSLEERSKILADFRE
Sbjct: 421 SKAQLRNIDDGAIRTMVFANTVETVEAVANILLVAGTECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
            GGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR
Sbjct: 481 KGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAA+RRAENLSQPVEAAFSRKRSFRNKLKKQGS KESVLS
Sbjct: 541 DLVAAIRRAENLSQPVEAAFSRKRSFRNKLKKQGSGKESVLS 581

BLAST of Cp4.1LG01g13070 vs. ExPASy TrEMBL
Match: A0A5A7SV31 (DEAD-box ATP-dependent RNA helicase 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00700 PE=4 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 515/600 (85.83%), Postives = 544/600 (90.67%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRS SAL+LERL  SSKLLFSFKNSNLLVP+SCSIRIGLVCL+QPCS+QSRAVGTVA
Sbjct: 1   MLLYRSVSALHLERLKLSSKLLFSFKNSNLLVPNSCSIRIGLVCLKQPCSRQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAA+  ADGIG+DTFYAAEGVSWTSLGVSD VSRAL S G+QRPSLIQAACVPSIMSGN
Sbjct: 61  ASAASTAADGIGKDTFYAAEGVSWTSLGVSDTVSRALGSVGMQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKIC+E  +DKLTDGDQDLAS+NKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICSEHGEDKLTDGDQDLASLNKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMAN LCDEHGKSLLRVT +CGRQGWPV+KPDI+VSTPAALLNYIEPNRSRR  FL AV
Sbjct: 181 VQMANALCDEHGKSLLRVTAICGRQGWPVHKPDIVVSTPAALLNYIEPNRSRRSAFLRAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKE PERLMELEADP SQL +QDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKEFPERLMELEADPLSQLIVQDE 300

Query: 301 SDIETETISEGEGE------------------GEGEESYDEDVDSNNIQEKTEYSSRRVK 360
            D++TET SEGEGE                  GEGEESY+E V+SNNIQ++TE  S +VK
Sbjct: 301 DDLQTETTSEGEGEKEEEGEEEGEGEEEGEGEGEGEESYNE-VESNNIQDETECVSGKVK 360

Query: 361 DWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRW 420
            WRR +KSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCH+PRLEQRW
Sbjct: 361 HWRRVRKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHNPRLEQRW 420

Query: 421 VEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRY 480
           VEV+ DNQVDELIKAVNQSKSQLR  D   IRTMVFANTVETVEAVANIL  AG ECFRY
Sbjct: 421 VEVSTDNQVDELIKAVNQSKSQLRVPDDGVIRTMVFANTVETVEAVANILLGAGKECFRY 480

Query: 481 HKDRSLEERSKILADFRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRT 540
           HKDRSLEERSKILADFRE GGV VCTDAAARGVDIPNV+HVIQADFATSAVDFLHRIGRT
Sbjct: 481 HKDRSLEERSKILADFREEGGVFVCTDAAARGVDIPNVSHVIQADFATSAVDFLHRIGRT 540

Query: 541 GRAGQCGLVTSMYTKANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           GRAGQ GLVTS+YTKANRDLV+AVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESV++
Sbjct: 541 GRAGQRGLVTSLYTKANRDLVSAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVMA 599

BLAST of Cp4.1LG01g13070 vs. ExPASy TrEMBL
Match: A0A1S3BFF8 (DEAD-box ATP-dependent RNA helicase 22 OS=Cucumis melo OX=3656 GN=LOC103489039 PE=4 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 515/600 (85.83%), Postives = 544/600 (90.67%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRS SAL+LERL  SSKLLFSFKNSNLLVP+SCSIRIGLVCL+QPCS+QSRAVGTVA
Sbjct: 1   MLLYRSVSALHLERLKLSSKLLFSFKNSNLLVPNSCSIRIGLVCLKQPCSRQSRAVGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           ASAA+  ADGIG+DTFYAAEGVSWTSLGVSD VSRAL S G+QRPSLIQAACVPSIMSGN
Sbjct: 61  ASAASTAADGIGKDTFYAAEGVSWTSLGVSDTVSRALGSVGMQRPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLINKIC+E  +DKLTDGDQDLAS+NKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINKICSEHGEDKLTDGDQDLASLNKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMAN LCDEHGKSLLRVT +CGRQGWPV+KPDI+VSTPAALLNYIEPNRSRR  FL AV
Sbjct: 181 VQMANALCDEHGKSLLRVTAICGRQGWPVHKPDIVVSTPAALLNYIEPNRSRRSAFLRAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKE PERLMELEADP SQL +QDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKEFPERLMELEADPLSQLIVQDE 300

Query: 301 SDIETETISEGEGE------------------GEGEESYDEDVDSNNIQEKTEYSSRRVK 360
            D++TET SEGEGE                  GEGEESY+E V+SNNIQ++TE  S +VK
Sbjct: 301 DDLQTETTSEGEGEKEEEGEEEGEGEEEGEGEGEGEESYNE-VESNNIQDETECVSGKVK 360

Query: 361 DWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRW 420
            WRR +KSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCH+PRLEQRW
Sbjct: 361 HWRRVRKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHNPRLEQRW 420

Query: 421 VEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRY 480
           VEV+ DNQVDELIKAVNQSKSQLR  D   IRTMVFANTVETVEAVANIL  AG ECFRY
Sbjct: 421 VEVSTDNQVDELIKAVNQSKSQLRVPDDGVIRTMVFANTVETVEAVANILLGAGKECFRY 480

Query: 481 HKDRSLEERSKILADFRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRT 540
           HKDRSLEERSKILADFRE GGV VCTDAAARGVDIPNV+HVIQADFATSAVDFLHRIGRT
Sbjct: 481 HKDRSLEERSKILADFREEGGVFVCTDAAARGVDIPNVSHVIQADFATSAVDFLHRIGRT 540

Query: 541 GRAGQCGLVTSMYTKANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           GRAGQ GLVTS+YTKANRDLV+AVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESV++
Sbjct: 541 GRAGQRGLVTSLYTKANRDLVSAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVMA 599

BLAST of Cp4.1LG01g13070 vs. ExPASy TrEMBL
Match: A0A6J1DBC8 (DEAD-box ATP-dependent RNA helicase 22 OS=Momordica charantia OX=3673 GN=LOC111019135 PE=4 SV=1)

HSP 1 Score: 974 bits (2517), Expect = 0.0
Identity = 509/582 (87.46%), Postives = 542/582 (93.13%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASSSKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGTVA 60
           MLLYRSASAL+LERLA  SKLLFSFKN N LV +SCSIRIGLV LRQ CS++SRA GTVA
Sbjct: 1   MLLYRSASALHLERLALPSKLLFSFKNYNSLVSTSCSIRIGLVSLRQRCSEKSRAAGTVA 60

Query: 61  ASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGN 120
           AS A+  A+G GRDTF+AAEGVSWTSLGVSDNVSRALVS+GIQ PSLIQAACVPSIMSGN
Sbjct: 61  ASTASTAAEGTGRDTFFAAEGVSWTSLGVSDNVSRALVSSGIQMPSLIQAACVPSIMSGN 120

Query: 121 DVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQV 180
           DVVIAAETGSGKTHGYLVPLIN+IC+E  D+KLT  DQDLAS NKLSLVLCPNVMLCEQV
Sbjct: 121 DVVIAAETGSGKTHGYLVPLINRICSEHGDNKLTGSDQDLASFNKLSLVLCPNVMLCEQV 180

Query: 181 VQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLCAV 240
           VQMANTLCDEHGKSLLRVT VCGRQGWP+++PDIIVSTPAALLNYIEPNRSRR+ FL AV
Sbjct: 181 VQMANTLCDEHGKSLLRVTAVCGRQGWPIHEPDIIVSTPAALLNYIEPNRSRRLDFLRAV 240

Query: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDE 300
           KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESP+RL+ELEADPSS+L LQDE
Sbjct: 241 KHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSRSKESPKRLVELEADPSSELILQDE 300

Query: 301 SDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFV 360
           +D++TETISEGE +GEGEESY+EDVDSN+IQE+TE  S RVKDWRR +K YERSKQYIFV
Sbjct: 301 NDLQTETISEGEEDGEGEESYEEDVDSNDIQEQTESVSGRVKDWRRVRKFYERSKQYIFV 360

Query: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQ 420
           AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEV++DNQVDELIKAVNQ
Sbjct: 361 AATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVSLDNQVDELIKAVNQ 420

Query: 421 SKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE 480
            KS+ RN DG AIRTMVFANTVETVEAV N+L  AG ECFRYHKDRSLEERSKILADFRE
Sbjct: 421 PKSKQRNPDGGAIRTMVFANTVETVEAVDNVLLRAGIECFRYHKDRSLEERSKILADFRE 480

Query: 481 NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANR 540
            GGV VCTDAAARGVDIPNV+HVIQADFATSAVDFLHRIGRTGRAGQ GLVTS+YTKANR
Sbjct: 481 KGGVFVCTDAAARGVDIPNVSHVIQADFATSAVDFLHRIGRTGRAGQYGLVTSLYTKANR 540

Query: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESVLS 582
           DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKES L+
Sbjct: 541 DLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAKESALA 582

BLAST of Cp4.1LG01g13070 vs. TAIR 10
Match: AT1G59990.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 594.7 bits (1532), Expect = 7.8e-170
Identity = 335/581 (57.66%), Postives = 414/581 (71.26%), Query Frame = 0

Query: 1   MLLYRSASALYLERLASS--SKLLFSFKNSNLLVPSSCSIRIGLVCLRQPCSKQSRAVGT 60
           M+L RS S L+L  ++SS  SKLL      +  +    S+   L  L +   K  R    
Sbjct: 1   MILSRSVSVLHLCGVSSSAPSKLLSQRFKVSFALAYGSSVSFRLSSLNRSDRKWVRGF-- 60

Query: 61  VAASAATPVADGIGRDTFYAAEGVSWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMS 120
             ASA     +  G DTF+A   VSW SLG+SDNVS AL  +G  RPSL QA C+PSI+S
Sbjct: 61  --ASATEAEVEKKGNDTFFADHTVSWKSLGLSDNVSIALRDSGFDRPSLTQAVCIPSILS 120

Query: 121 GNDVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCE 180
           G DV++AAETGSGKTHGYL P+I+++ N   D ++T+ ++    +  +SL+LCPNVMLCE
Sbjct: 121 GKDVIVAAETGSGKTHGYLAPIIDQLTNTALDSEVTNREERPFPLKNISLILCPNVMLCE 180

Query: 181 QVVQMANTLCDEHGKSLLRVTTVCGRQGWPVYKPDIIVSTPAALLNYIEPNRSRRMKFLC 240
           QVV+M N L DE G  LLRV  VCG QGWP   PDIIVSTPAALLN IEP R+RR++FL 
Sbjct: 181 QVVRMVNGLVDEDGNPLLRVEAVCGSQGWPDRLPDIIVSTPAALLNNIEPKRNRRLEFLR 240

Query: 241 AVKHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLSR-SKESPERLMELEAD-PSSQLT 300
            VK+VVFDEADMLL GSFQN++IRLIN+LRF+EK +SR +K +  R ME++A  P   L 
Sbjct: 241 CVKYVVFDEADMLLCGSFQNQIIRLINMLRFDEKQVSRLAKSNLGRPMEIDASVPQIDLE 300

Query: 301 LQDESDIETETISEGEGEGEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQ 360
            +D+++ +  +ISE E E E EE  D+     +++   E  S   K WRR +K Y RSKQ
Sbjct: 301 NEDDAEFDEGSISEEEDEEEEEEYLDDIAQMPSVE--AEAGSDTKKGWRRVRKIYTRSKQ 360

Query: 361 YIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIK 420
           YIF+AATLPVNGKKTAG +L+ MF DA WVSG +LH +SPRL+Q+WVEVTVD+QVD LI+
Sbjct: 361 YIFIAATLPVNGKKTAGGILKHMFQDAVWVSGNFLHRNSPRLKQKWVEVTVDSQVDALIE 420

Query: 421 AVNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILA 480
           AV        N + +  RTMVFANTVE VEAVA+IL  A  +C+RYHK+  L+ER+ ILA
Sbjct: 421 AVK------NNNNTNTERTMVFANTVEAVEAVADILEKASIQCYRYHKNHKLDERANILA 480

Query: 481 DFRENGGVLVCTDAAARGVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYT 540
           DFRE GGV VCTDAAARGVD+PNV+HVIQADFA+SAVDFLHRIGRT RAGQ G VTS+YT
Sbjct: 481 DFRETGGVFVCTDAAARGVDVPNVSHVIQADFASSAVDFLHRIGRTARAGQYGTVTSLYT 540

Query: 541 KANRDLVAAVRRAENLSQPVEAAFSRKRSFRNKLKKQGSAK 578
           +ANRDLV A+R A  + QPVE AFSRKR FRNK+KK+   K
Sbjct: 541 EANRDLVEAIREAVKMGQPVETAFSRKRGFRNKVKKRAFLK 569

BLAST of Cp4.1LG01g13070 vs. TAIR 10
Match: AT2G33730.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.5e-27
Identity = 120/461 (26.03%), Postives = 196/461 (42.52%), Query Frame = 0

Query: 83  SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLIN 142
           SW    ++  + +A+  AG ++PS IQ A +P  +   DV+  AETGSGKT  +++P++ 
Sbjct: 314 SWEESKLTSELLKAVERAGYKKPSPIQMAAIPLGLQQRDVIGIAETGSGKTAAFVLPMLA 373

Query: 143 KICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVC 202
            I       +L    ++  +    ++V+ P   L +Q+ +         G    RVT++ 
Sbjct: 374 YI------SRLPPMSEENETEGPYAVVMAPTRELAQQIEEETVKFAHYLG---FRVTSIV 433

Query: 203 G-----RQGWPVYKP-DIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVGSF 262
           G      QG  + +  +I+++TP  L++ +E    RR   L    +VV DEAD ++   F
Sbjct: 434 GGQSIEEQGLKITQGCEIVIATPGRLIDCLE----RRYAVLNQCNYVVLDEADRMIDMGF 493

Query: 263 QNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEGEGE 322
           + +V  +                       L+A PSS L  ++E                
Sbjct: 494 EPQVAGV-----------------------LDAMPSSNLKPENE---------------- 553

Query: 323 GEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVL 382
                +E++D   I   T                      Y+F +AT+P   ++ A   L
Sbjct: 554 -----EEELDEKKIYRTT----------------------YMF-SATMPPGVERLARKYL 613

Query: 383 RKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTM 442
           R         +GK     +  + Q  + +    +   L K +++   +           +
Sbjct: 614 RNPVVVTIGTAGK----TTDLISQHVIMMKESEKFFRLQKLLDELGEK---------TAI 673

Query: 443 VFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFR-ENGGVLVCTDAAARGV 502
           VF NT +  +++A  L  AG      H  +S E+R   L  FR +   VLV TD   RG+
Sbjct: 674 VFVNTKKNCDSIAKNLDKAGYRVTTLHGGKSQEQREISLEGFRAKRYNVLVATDVVGRGI 681

Query: 503 DIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYT 537
           DIP+VAHVI  D       + HRIGRTGRAG+ G+ TS  T
Sbjct: 734 DIPDVAHVINYDMPKHIEMYTHRIGRTGRAGKSGVATSFLT 681

BLAST of Cp4.1LG01g13070 vs. TAIR 10
Match: AT4G09730.1 (RH39 )

HSP 1 Score: 117.1 bits (292), Expect = 4.8e-26
Identity = 115/485 (23.71%), Postives = 207/485 (42.68%), Query Frame = 0

Query: 83  SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLIN 142
           ++  LG+S+ V  AL    I+ P+ IQ   +P++M    VV+ + TGSGKT  YL+P++ 
Sbjct: 113 NFQELGLSEEVMGALQELNIEVPTEIQCIGIPAVMERKSVVLGSHTGSGKTLAYLLPIVQ 172

Query: 143 KICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVC 202
            +    R+D+   G +        ++VLCP   L EQV ++A ++   H      +    
Sbjct: 173 LM----REDEANLGKKTKPR-RPRTVVLCPTRELSEQVYRVAKSI--SHHARFRSILVSG 232

Query: 203 GRQGWPVYKP-----DIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVGSFQ 262
           G +  P         D++V TP  +L +IE            + ++V DEAD +    F 
Sbjct: 233 GSRIRPQEDSLNNAIDMVVGTPGRILQHIEEGN----MVYGDIAYLVLDEADTMFDRGFG 292

Query: 263 NKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEGEGEG 322
            ++ + +  L                                                  
Sbjct: 293 PEIRKFLAPLN------------------------------------------------- 352

Query: 323 EESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVLR 382
                                      +RA K+ ++  Q + V AT+ +  +K    ++ 
Sbjct: 353 ---------------------------QRALKTNDQGFQTVLVTATMTMAVQK----LVD 412

Query: 383 KMFPDASWVSGKYLHCHSPRLEQRWVEVT-VDNQVDELIKAVNQSKSQLRNIDGSAIRTM 442
           + F     +    LH         +++++  +++++ L++ +  S ++     GS  + M
Sbjct: 413 EEFQGIEHLRTSTLHKKIANARHDFIKLSGGEDKLEALLQVLEPSLAK-----GS--KVM 472

Query: 443 VFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRENGG---VLVCTDAAAR 502
           VF NT+ +  AV + LS        YH +   E+R + L  F++  G    LVCTD AAR
Sbjct: 473 VFCNTLNSSRAVDHYLSENQISTVNYHGEVPAEQRVENLKKFKDEEGDCPTLVCTDLAAR 498

Query: 503 GVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKANRDLVAAVRRAENLS 559
           G+D+ +V HV+  DF  +++D+LHR GRT R G  G VTS+ ++ ++ L A +  A   +
Sbjct: 533 GLDL-DVDHVVMFDFPKNSIDYLHRTGRTARMGAKGKVTSLVSRKDQMLAARIEEAMRNN 498

BLAST of Cp4.1LG01g13070 vs. TAIR 10
Match: AT1G55150.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.6e-24
Identity = 119/493 (24.14%), Postives = 195/493 (39.55%), Query Frame = 0

Query: 83  SWTSLGVSDNVSRALVSAGIQRPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLIN 142
           S+  +G  D V   +  AG   P+ IQ+   P  M G D++  AETGSGKT  YL+P I 
Sbjct: 100 SFRDVGFPDYVLEEVKKAGFTEPTPIQSQGWPMAMKGRDLIGIAETGSGKTLSYLLPAIV 159

Query: 143 KICNEQRDDKLTDGDQDLASINKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVC 202
            +        L  GD        + LVL P   L  Q+ Q A+        S  ++ T C
Sbjct: 160 HV---NAQPMLAHGD------GPIVLVLAPTRELAVQIQQEASKF-----GSSSKIKTTC 219

Query: 203 GRQGWPVYKP--------DIIVSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVG 262
              G P            +I+++TP  L++ +E N +     L  V ++V DEAD +L  
Sbjct: 220 IYGGVPKGPQVRDLQKGVEIVIATPGRLIDMMESNNTN----LRRVTYLVLDEADRMLDM 279

Query: 263 SFQNKVIRLINLLRFEEKLLSRSKESPERLMELEADPSSQLTLQDESDIETETISEGEGE 322
            F  ++ ++++ +R + + L  S   P+ + +L                           
Sbjct: 280 GFDPQIRKIVSHIRPDRQTLYWSATWPKEVEQL--------------------------- 339

Query: 323 GEGEESYDEDVDSNNIQEKTEYSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGA 382
                                                  SK++++    + +        
Sbjct: 340 ---------------------------------------SKKFLYNPYKVIIGSSDLKAN 399

Query: 383 VLRKMFPDASWVSGKYLHCHSPRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAIR 442
              +   D    S KY                  N++ +L++ +         +DGS  R
Sbjct: 400 RAIRQIVDVISESQKY------------------NKLVKLLEDI---------MDGS--R 459

Query: 443 TMVFANTVETVEAVANILSVAGTECFRYHKDRSLEERSKILADFRE-NGGVLVCTDAAAR 502
            +VF +T +  + +   L + G      H D+S  ER  +L++FR     ++  TD AAR
Sbjct: 460 ILVFLDTKKGCDQITRQLRMDGWPALSIHGDKSQAERDWVLSEFRSGKSPIMTATDVAAR 479

Query: 503 GVDIPNVAHVIQADFATSAVDFLHRIGRTGRAGQCGLVTSMYTKAN----RDLVAAVRRA 562
           G+D+ +V +VI  DF  S  D++HRIGRTGRAG  G   + +T AN    ++L   ++ A
Sbjct: 520 GLDVKDVKYVINYDFPGSLEDYVHRIGRTGRAGAKGTAYTFFTVANARFAKELTNILQEA 479

BLAST of Cp4.1LG01g13070 vs. TAIR 10
Match: AT3G58570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 3.8e-23
Identity = 107/445 (24.04%), Postives = 182/445 (40.90%), Query Frame = 0

Query: 104 RPSLIQAACVPSIMSGNDVVIAAETGSGKTHGYLVPLINKICNEQRDDKLTDGDQDLASI 163
           +P+ +Q   +P + +G D++  A+TGSGKT  +  P+I+ I  +Q  ++     + +  +
Sbjct: 168 KPTPVQRNAIPILAAGRDLMACAQTGSGKTAAFCFPIISGIMKDQHIER----PRGVRGV 227

Query: 164 NKLSLVLCPNVMLCEQVVQMANTLCDEHGKSLLRVTTVCGRQGWPVYKP--------DII 223
             L+++L P   L  Q+   A     + G     V  V    G PV +         DI+
Sbjct: 228 YPLAVILSPTRELACQIHDEARKFSYQTG-----VKVVVAYGGTPVNQQIRELERGVDIL 287

Query: 224 VSTPAALLNYIEPNRSRRMKFLCAVKHVVFDEADMLLVGSFQNKVIRLINLLRFEEKLLS 283
           V+TP  L + +E  R      L  V+ +  DEAD +L   F+ ++ +++  +        
Sbjct: 288 VATPGRLNDLLERGRVS----LQMVRFLALDEADRMLDMGFEPQIRKIVQQM-------- 347

Query: 284 RSKESPERLMELEADPSSQLTLQDESDIETETISEGEGEGEGEESYDEDVDSNNIQEKTE 343
                                                           D+    +++   
Sbjct: 348 ------------------------------------------------DMPPPGVRQTML 407

Query: 344 YSSRRVKDWRRAQKSYERSKQYIFVAATLPVNGKKTAGAVLRKMFPDASWVSGKYLHCHS 403
           +S+   ++ +R    +     YIF+A      G  T   V R           +++H   
Sbjct: 408 FSATFPREIQRLASDF--LSNYIFLAVGRV--GSSTDLIVQRV----------EFVHDSD 467

Query: 404 PRLEQRWVEVTVDNQVDELIKAVNQSKSQLRNIDGSAIRTMVFANTVETVEAVANILSVA 463
            R           + + +L+ A  ++ +Q     G    T+VF  T +  +++ N L + 
Sbjct: 468 KR-----------SHLMDLLHAQRENGNQ-----GKQALTLVFVETKKGADSLENWLCIN 513

Query: 464 GTECFRYHKDRSLEERSKILADFRE-NGGVLVCTDAAARGVDIPNVAHVIQADFATSAVD 523
           G      H DRS +ER   L  F+     +LV TD AARG+DIP+VAHV+  D      D
Sbjct: 528 GFPATTIHGDRSQQEREVALRSFKTGRTPILVATDVAARGLDIPHVAHVVNFDLPNDIDD 513

Query: 524 FLHRIGRTGRAGQCGLVTSMYTKAN 540
           ++HRIGRTGRAG  GL T+ +   N
Sbjct: 588 YVHRIGRTGRAGNSGLATAFFNDNN 513