Cp4.1LG01g10970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g10970 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 9.0e-300
Identity = 515/714 (72.13%), Postives = 589/714 (82.49%), Query Frame = 0

Query: 4   LASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFNG 63
           LAS   S  D + IYIVYMG KLEDP SAHLHHRAMLEQVVGSTF P+SVL+TY RSFNG
Sbjct: 20  LASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNG 79

Query: 64  FVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGVL 123
           F V+LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDFLGFP  V R  Q+ESNIVVGVL
Sbjct: 80  FAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVL 139

Query: 124 DSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFRCNRKLIGGRAYRIGRPILPGDVGSPR 183
           D+GIWPESPSF+D+GF  PP KW+G+C+ S NFRCNRK+IG R+Y IGRPI PGDV  PR
Sbjct: 140 DTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPR 199

Query: 184 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 243
           DT+GHGTHTAST AGGLVSQA+LYGLG+GTARGGVP ARIAAYKVCW DGCSD DILAA+
Sbjct: 200 DTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAY 259

Query: 244 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 303
           DDAIADGVDI+SLSVG + PR YF D IAIGSFHA++ GILTSNSAGN GP  FTT SLS
Sbjct: 260 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 319

Query: 304 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 363
           PWLLSVAAS MDRKFVT+VQ+GNG  ++G+SINTFD N  YPLV   DIPN GF+ STSR
Sbjct: 320 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYYPLVSGRDIPNTGFDKSTSR 379

Query: 364 YCEKNSVNPSLAAGKILLCD-SVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 423
           +C   SVNP+L  GKI++C+ S  P   F S    AG+LM + TRD A SYP P++VL P
Sbjct: 380 FCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDP 439

Query: 424 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 483
           ND +A  RYI S   P ATIFKS    ++SAP+VVSFSSRGPN  T D++KPD+SGPGVE
Sbjct: 440 NDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVE 499

Query: 484 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 543
           ILAAWP VAP+ G++  R++L+NIISGTSMSCPH+TG+A YVKT+NPT SPAAIKSAL+T
Sbjct: 500 ILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMT 559

Query: 544 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 603
           TASPM+AR + QAEFAYG+GHVNPLKA+ PGLVYDANESDYV FLCGQGY+T  VRRITG
Sbjct: 560 TASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITG 619

Query: 604 DNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLS 663
           D SACTSGN GRVWDLNYPSF +S+S ++T NQYF RTLT+VA   STYRA ISAPQGL+
Sbjct: 620 DYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLT 679

Query: 664 ITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVAL 716
           I+VNP  LSFNG+GDRKSF LTV G++   + SASL WSDGVH VRSPIT+ +L
Sbjct: 680 ISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITITSL 730

BLAST of Cp4.1LG01g10970 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.4e-172
Identity = 333/715 (46.57%), Postives = 459/715 (64.20%), Query Frame = 0

Query: 13  DDRKIYIVYMGSKLE----DPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFNGFVVRL 72
           DD+++YIVYMGS        P+S H++   +L++V G +     ++ +Y RSFNGF  RL
Sbjct: 27  DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQEVTGESSIEGRLVRSYKRSFNGFAARL 86

Query: 73  TEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNV--GRVPQLESNIVVGVLDSG 132
           TE E + +A   GVVSVF N+   L TT SWDF+G  + +   R P +ES+ ++GV+DSG
Sbjct: 87  TESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSG 146

Query: 133 IWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILPGDVGSPRDTD 192
           I PES SF+D GFG PP KW+G C    NF CN KLIG R Y              RD D
Sbjct: 147 ITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY---------TSEGTRDMD 206

Query: 193 GHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAFDDA 252
           GHGTHTAST AG  V  AS +G+G GT RGGVP++R+AAYKVC   GCS   +L+AFDDA
Sbjct: 207 GHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDA 266

Query: 253 IADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPWL 312
           IADGVD++++S+G      + NDPIAIG+FHA+  G+LT NSAGN GPK  + + ++PW+
Sbjct: 267 IADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWI 326

Query: 313 LSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNLGFNGSTSRYC 372
           L+VAAS  +R FVT+V LGNG    G S+N ++M    YPLVY     +   +  ++  C
Sbjct: 327 LTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLC 386

Query: 373 EKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPNDG 432
           E + V+ S   GKIL+C       I  S GA+ G++ +    D A  +P PAA L   D 
Sbjct: 387 ELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GLIYRTPKPDVAFIHPLPAAGLLTEDF 446

Query: 433 IAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEILA 492
            ++  Y+ ST  P A + K+ A  + ++P++ SFSSRGPN +  DILKPD++ PGVEILA
Sbjct: 447 ESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILA 506

Query: 493 AWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTAS 552
           A+ P A      D+R   Y+++SGTSMSCPH+ GVA YVKTFNP  SP+ I+SA++TTA 
Sbjct: 507 AYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAW 566

Query: 553 PMSARQH--SQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 612
           P++A     +  EFAYG+GHV+P+ A NPGLVY+ ++SD+++FLCG  Y++ +++ I+G+
Sbjct: 567 PVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGE 626

Query: 613 NSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYRAKISAPQG-- 672
              C+        +LNYPS +  +SG+  T    F RTLTNV    STY +K+ A  G  
Sbjct: 627 TVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSK 686

Query: 673 LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVRSPITV 713
           L + + P+ LSF  + +++SF +TV G+   S+  SSA+L WSDG H+VRSPI V
Sbjct: 687 LDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVV 727

BLAST of Cp4.1LG01g10970 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.9e-172
Identity = 340/717 (47.42%), Postives = 459/717 (64.02%), Query Frame = 0

Query: 14  DRKIYIVYMGSKLEDPSSAHL----HHRAMLEQVVGSTFDPQSVLYTYTRSFNGFVVRLT 73
           D+++YIVYMG+    PS        HH ++L+ V G +     ++  Y RSFNGF  RLT
Sbjct: 30  DKQVYIVYMGAL---PSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLT 89

Query: 74  EEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLESNIVVGVLDSGI 133
           E E + +AS + VVSVF ++N +L TT SW+F+G  +     R P +ES+ ++GV+DSGI
Sbjct: 90  ESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGI 149

Query: 134 WPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILPGDVGSPRDTDG 193
           +PES SF+  GFG PP KW+G C+  +NF CN KLIG R Y    P L G   S RD  G
Sbjct: 150 YPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY---TPKLEGFPESARDNTG 209

Query: 194 HGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKD--GCSDADILAAFDD 253
           HG+HTAS  AG  V   S YGLG GT RGGVP+ARIA YKVC      C+   ILAAFDD
Sbjct: 210 HGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDD 269

Query: 254 AIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPW 313
           AIAD VDI+++S+G+     +  D +AIG+FHA+  GILT N AGN GP+  T  S++PW
Sbjct: 270 AIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPW 329

Query: 314 LLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMN-LQYPLVYAGDIPNLGFNGSTSRY 373
           L +VAAS M+R F+T+V LGNG    G S+N+FD+N  +YPLVY G   +   + S++ +
Sbjct: 330 LFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY-GKSASSRCDASSAGF 389

Query: 374 CEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPND 433
           C    ++     GKI+LCD+        + GA+A I+   Y  D A  +  P +VL  +D
Sbjct: 390 CSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPY-EDAASVFSFPVSVLSEDD 449

Query: 434 GIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEIL 493
              V  Y+ ST  P A + KS    +  AP+V S+SSRGPN L +DILKPD++ PG EIL
Sbjct: 450 YNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEIL 509

Query: 494 AAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTA 553
           AA+ P  P     D+R   Y +ISGTSMSCPH+ GVA Y+KTF+P  SP+ I+SA++TTA
Sbjct: 510 AAYSPYVP-PSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTA 569

Query: 554 SPMSAR---QHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRIT 613
            PM+A     +  AEFAYGAGHV+P+ AI+PGLVY+AN+SD+++FLCG  Y+   +R I+
Sbjct: 570 WPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLIS 629

Query: 614 GDNSACTSGNIGRVW-DLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQG 673
           GD+S+CT      +  +LNYPS +  +SGT+     F RT+TNV R  +TY+AK+   + 
Sbjct: 630 GDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK- 689

Query: 674 LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVRSPITVVA 715
           L + V P  LS   + ++KSF +TV G    ++++ SA L WSDGVH VRSPI V A
Sbjct: 690 LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Cp4.1LG01g10970 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 5.5e-172
Identity = 341/725 (47.03%), Postives = 461/725 (63.59%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLED----PSSAHLHHRAMLEQVVGSTFDPQSVLYTYT 62
           +L+S S    +D ++YIVYMGS        P+S H+   ++L+QV G +     ++ +Y 
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIEGRLVRSYK 77

Query: 63  RSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLES 122
           RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDF+G  +  N  R   +ES
Sbjct: 78  RSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIES 137

Query: 123 NIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILP 182
           + ++GV+D+GIWPES SF+D GFG PP KW+G C    NF CN KLIG R Y        
Sbjct: 138 DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY-------- 197

Query: 183 GDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSD 242
                 RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKVC   GCS 
Sbjct: 198 -TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSS 257

Query: 243 ADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKD 302
             +L++FDDAIADGVD++++S+G   P  + +DPIAIG+FHA+  GILT +SAGN GPK 
Sbjct: 258 EALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKP 317

Query: 303 FTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNL 362
            T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N FDM   +YPLVY     + 
Sbjct: 318 TTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASS 377

Query: 363 GFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPA 422
             +  T+  C    +N S   GKIL+C       I  S GAIA I+ ++   D A ++  
Sbjct: 378 ACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHL 437

Query: 423 PAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPD 482
           PA+ L   D  ++  YI S   P A + K+    + ++P++ SFSSRGPN +  DILKPD
Sbjct: 438 PASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPD 497

Query: 483 LSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAA 542
           ++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF P  SP+ 
Sbjct: 498 ITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 557

Query: 543 IKSALITTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYS 602
           I+SA++TTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++FLCG  Y+
Sbjct: 558 IQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 617

Query: 603 TSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYR 662
           +  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV    STY+
Sbjct: 618 SKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYK 677

Query: 663 AKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVR 713
           +K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L WSDG H+VR
Sbjct: 678 SKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVR 728

BLAST of Cp4.1LG01g10970 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 2.7e-171
Identity = 326/707 (46.11%), Postives = 454/707 (64.21%), Query Frame = 0

Query: 17  IYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFNGFVVRLTEEEAQEI 76
           +YIVYMG+  E   S   HH ++L+++VG+      ++ +Y RSFNGF   L++ E+Q++
Sbjct: 32  VYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKL 91

Query: 77  ASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGVLDSGIWPESPSFND 136
            + + VVSVF ++++ L TTRSWDF+GF +   R    ES+++VGV+DSGIWPES SF+D
Sbjct: 92  QNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDD 151

Query: 137 DGFGSPPSKWRGSCQVS-NFRCNRKLIGGRAYRIGRPILPGDVGSPRDTDGHGTHTASTV 196
           +GFG PP KW+GSC+    F CN KLIG R Y            S RD +GHGTHTAST 
Sbjct: 152 EGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTA 211

Query: 197 AGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAFDDAIADGVDIVSL 256
           AG  V  AS YGL  GTARGGVPSARIAAYKVC+ + C+D DILAAFDDAIADGVD++S+
Sbjct: 212 AGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISI 271

Query: 257 SVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDR 316
           S+ +       N  +AIGSFHA+  GI+T+ SAGN GP   +  ++SPW+++VAASG DR
Sbjct: 272 SISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDR 331

Query: 317 KFVTRVQLGNGNIYEGISINTFDMN-LQYPLVYAGDIPNLGFNGSTSRYCEKNSVNPSLA 376
           +F+ RV LGNG    GIS+NTF++N  ++P+VY  ++ +   + + + YC    V+  L 
Sbjct: 332 QFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELV 391

Query: 377 AGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPNDGIAVYRYIRST 436
            GKI+LCD  +        GAI  I+      D+A   P PA+ L   D  ++  YI S 
Sbjct: 392 KGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESA 451

Query: 437 IIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVA---P 496
             P A I ++    D  AP V SFSSRGP+ +  ++LKPD+S PG+EILAA+ PVA    
Sbjct: 452 EPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSS 511

Query: 497 IAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTASPMSARQH 556
               +D RS  Y+++SGTSM+CPH+ GVA YVK+F+P  SP+AIKSA++TTA+PM+ +++
Sbjct: 512 FLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKN 571

Query: 557 SQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNI 616
            + EFAYG+G +NP KA +PGLVY+    DY+  LC +G+ ++ +   +G N  C+    
Sbjct: 572 PEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT- 631

Query: 617 GRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQ-GLSITVNPTALS 676
             V DLNYP+    +S     N  F RT+TNV    STY+A +   Q  L I++ P  L 
Sbjct: 632 -EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILR 691

Query: 677 FNGIGDRKSFRLTVGGTVSQ--SISSASLEWSDGVHSVRSPITVVAL 716
           F  + ++KSF +T+ G   +  S  S+S+ WSDG HSVRSPI   ++
Sbjct: 692 FGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Cp4.1LG01g10970 vs. NCBI nr
Match: XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1425 bits (3689), Expect = 0.0
Identity = 715/715 (100.00%), Postives = 715/715 (100.00%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN
Sbjct: 25  VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 84

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV
Sbjct: 85  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 144

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR
Sbjct: 145 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 204

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF
Sbjct: 205 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 264

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS
Sbjct: 265 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 324

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 362
           PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR
Sbjct: 325 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 384

Query: 363 YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN 422
           YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN
Sbjct: 385 YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN 444

Query: 423 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI 482
           DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI
Sbjct: 445 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI 504

Query: 483 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT 542
           LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT
Sbjct: 505 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT 564

Query: 543 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 602
           ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD
Sbjct: 565 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 624

Query: 603 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 662
           NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI
Sbjct: 625 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 684

Query: 663 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK
Sbjct: 685 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 739

BLAST of Cp4.1LG01g10970 vs. NCBI nr
Match: KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1380 bits (3571), Expect = 0.0
Identity = 691/715 (96.64%), Postives = 703/715 (98.32%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRAMLEQVVGSTFDPQSVLYTY RSFN
Sbjct: 18  VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFN 77

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFPQNVGRV QLESNIVVGV
Sbjct: 78  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVGRVQQLESNIVVGV 137

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRG CQ SNF CNRKLIGGRAY IGRPI PGDVGSPR
Sbjct: 138 LDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGRAYHIGRPIPPGDVGSPR 197

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAYKVCW+DGCSDADILAAF
Sbjct: 198 DTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKVCWEDGCSDADILAAF 257

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVDIVSLSVGSS+PRPYF+DPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS
Sbjct: 258 DDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 317

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 362
           PWLLSVAASGMDRKFVTRVQLGNGNIY+GISINTF+MNLQ+PLVYAGDIPNLGFNGSTSR
Sbjct: 318 PWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLVYAGDIPNLGFNGSTSR 377

Query: 363 YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN 422
           YCE+NSVNP LAAGKILLCDSVIP S FSSFGAIAGILMQAYTRDNARSYPAPAAVL PN
Sbjct: 378 YCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTRDNARSYPAPAAVLDPN 437

Query: 423 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI 482
           DGIAVYRYIRSTIIPTATIFKSVAGQDSSAP+VVSFSSRGPNALTNDILKPDLSGPGVEI
Sbjct: 438 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEI 497

Query: 483 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT 542
           LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSAL+TT
Sbjct: 498 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTT 557

Query: 543 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 602
           ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD
Sbjct: 558 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 617

Query: 603 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 662
           NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI
Sbjct: 618 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 677

Query: 663 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK
Sbjct: 678 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 732

BLAST of Cp4.1LG01g10970 vs. NCBI nr
Match: XP_022957348.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 684/715 (95.66%), Postives = 698/715 (97.62%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSH DDR+IYIVYMGSKL+DPSSAHLHHRAMLEQVVGSTFDPQSVLYTY RSFN
Sbjct: 25  VLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFN 84

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFPQNV RVPQLESNIVVGV
Sbjct: 85  GFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVDRVPQLESNIVVGV 144

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRG CQVSNF CNRKLIGGRAY IGRPI PGDVGSPR
Sbjct: 145 LDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKLIGGRAYHIGRPIAPGDVGSPR 204

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAYKVCW+DGC DADILAAF
Sbjct: 205 DTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKVCWEDGCWDADILAAF 264

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVDIVSLSVGS++PRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS
Sbjct: 265 DDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 324

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 362
           PWLLSVAASGMDRKFVTRVQLGNGNIY+GISINTF+MNLQ+PLVYAGDIPNLGFNGSTSR
Sbjct: 325 PWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLVYAGDIPNLGFNGSTSR 384

Query: 363 YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN 422
           YCE+NSVNP LAAGKILLCDSVIP S F SFGAI GILMQAYTRDNARSYPAPAAVL PN
Sbjct: 385 YCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIVGILMQAYTRDNARSYPAPAAVLDPN 444

Query: 423 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI 482
           DGIAVYRYIRSTIIPTATIFKSVAGQDSSAP+VVSFSSRGPNALTNDILKPDLSGPGVEI
Sbjct: 445 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEI 504

Query: 483 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT 542
           LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSAL+TT
Sbjct: 505 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTT 564

Query: 543 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 602
           ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGY T+MVRRITGD
Sbjct: 565 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYDTAMVRRITGD 624

Query: 603 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 662
           NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI
Sbjct: 625 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 684

Query: 663 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           TVNPTALSFNGIGDRKSFRLTV GTVSQSISSASLEWSDGVHSVRSPITVVALTK
Sbjct: 685 TVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDGVHSVRSPITVVALTK 739

BLAST of Cp4.1LG01g10970 vs. NCBI nr
Match: XP_022957349.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 574/712 (80.62%), Postives = 631/712 (88.62%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRAMLEQVVGSTFDPQSVLYTY RSFN
Sbjct: 18  VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFN 77

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFPQNV RVPQLESNIVVGV
Sbjct: 78  GFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVDRVPQLESNIVVGV 137

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRG CQVSNF CNRK+IG RAY IG+ I P +V SPR
Sbjct: 138 LDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGARAYHIGKSIAPDEVNSPR 197

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DT+GHGTHTAS VAGGLVSQASLY LG+GTARGG PSARIA YK+CWK GCSDADILAAF
Sbjct: 198 DTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKICWKHGCSDADILAAF 257

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVD +SLSVGSS+PRPYF+DPIAIGSFHA+K GILTSNSAGNEGPKD TTTSLS
Sbjct: 258 DDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNSAGNEGPKDSTTTSLS 317

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFD-MNLQYPLVYAGDIPNLGFNGSTS 362
           PWLLSVAA+ MDRKFVTRV+LG+G  ++G+SINTF+ M  QYPLVY GD+PN GF  S S
Sbjct: 318 PWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLVYGGDVPNRGFKRSIS 377

Query: 363 RYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 422
           RYC KNSVNP    GK+LLC++++P S+FSS GAI G++MQ+ TRDNA SYP PAA++  
Sbjct: 378 RYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTRDNAGSYPLPAALVSR 437

Query: 423 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 482
            D I ++R+ R T+ P A  FKS A QD SAP++ SFSSRGPN+L N ILKPDLSGPGVE
Sbjct: 438 QDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLANHILKPDLSGPGVE 497

Query: 483 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 542
           IL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVYVKTFNPTLSPAAIKSAL+T
Sbjct: 498 ILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMT 557

Query: 543 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 602
           TASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++YV FLCGQGY T+MVRRITG
Sbjct: 558 TASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITG 617

Query: 603 DNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQYFTRTLTNVARGGSTYRAKISAPQGL 662
           DNSACTSGNIGRVWDLN PSFA+S++ G++ +NQYFTRTLTNVA  GSTYRA ISAP+GL
Sbjct: 618 DNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGL 677

Query: 663 SITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITV 712
            ITVNP  LSFN IG+RKSF LTVGGTVS+ + SA L WSDGVHSVRSPIT+
Sbjct: 678 RITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGVHSVRSPITI 729

BLAST of Cp4.1LG01g10970 vs. NCBI nr
Match: BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 560/716 (78.21%), Postives = 631/716 (88.13%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           +LAS   S  D RKIYIVYMG+KLEDP SAHLHHRAMLE+VVGSTF P+SV+YTY RSFN
Sbjct: 23  LLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGSTFAPESVIYTYKRSFN 82

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GF V+LTEEEAQ+IA+KEGVVSVFLNQ NH+HTT SWDFLGFPQ V R+ Q+ESNIVVGV
Sbjct: 83  GFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQAVARMRQVESNIVVGV 142

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFRCNRKLIGGRAYRIGRPILPGDVGSP 182
           LDSGIWPESPSF D+GFG  PS W+G+CQ S NF CN K+IG RAYR+G  + PGDV SP
Sbjct: 143 LDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNNKIIGARAYRVGGTLPPGDVSSP 202

Query: 183 RDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAA 242
           RDTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAYK+CW DGCSDADILAA
Sbjct: 203 RDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKICWSDGCSDADILAA 262

Query: 243 FDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSL 302
           FDDAIADGV I+SLSVG S+ RPYFNDPIAIG+FHA+K+GILTSNSAGNEG K FTTTSL
Sbjct: 263 FDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILTSNSAGNEGSKFFTTTSL 322

Query: 303 SPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTS 362
           SPWLLSVAAS  DRKFVT VQLGNG IY+G +INTFDMN QYPLVYAG++PN+GFNGSTS
Sbjct: 323 SPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDMNNQYPLVYAGEVPNVGFNGSTS 382

Query: 363 RYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 422
           R+C  NSVNP L  GKILLCDS++PSS+FSSFGAIAG+LMQ YT DNARS+  PA VLHP
Sbjct: 383 RFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQGYT-DNARSHSVPATVLHP 442

Query: 423 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 482
            DG  VYRYI ST  PTA I      +D+SAP+V+SFSSRGPNALTND+LKPD++GPGVE
Sbjct: 443 QDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPNALTNDLLKPDITGPGVE 502

Query: 483 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 542
           ILAAWPP+API+GV++SRS+L+NIISGTSMSCPHL+ VAVYVKTFNP+ SPAAIKSAL+T
Sbjct: 503 ILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVKTFNPSWSPAAIKSALMT 562

Query: 543 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 602
           TASPM+AR +S+AEFAYG+GHVNPLKA+NPGLVYDA++SDYV FLCGQGY+T+M+RRITG
Sbjct: 563 TASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVKFLCGQGYNTAMLRRITG 622

Query: 603 DNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLS 662
           DNSACTS NIGRVWDLNYPSFA+++SG +++NQYFTRTLTNVA   STYRA IS PQGL+
Sbjct: 623 DNSACTSANIGRVWDLNYPSFALAVSGYQSLNQYFTRTLTNVASQASTYRATISVPQGLT 682

Query: 663 ITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           ITVNP  LSF+G GDRKSF LTV G+VSQ I S SL WSDGVHSVRSP+ VV LTK
Sbjct: 683 ITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGVHSVRSPLVVVVLTK 737

BLAST of Cp4.1LG01g10970 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 684/715 (95.66%), Postives = 698/715 (97.62%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSH DDR+IYIVYMGSKL+DPSSAHLHHRAMLEQVVGSTFDPQSVLYTY RSFN
Sbjct: 25  VLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFN 84

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFPQNV RVPQLESNIVVGV
Sbjct: 85  GFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVDRVPQLESNIVVGV 144

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRG CQVSNF CNRKLIGGRAY IGRPI PGDVGSPR
Sbjct: 145 LDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKLIGGRAYHIGRPIAPGDVGSPR 204

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAYKVCW+DGC DADILAAF
Sbjct: 205 DTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKVCWEDGCWDADILAAF 264

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVDIVSLSVGS++PRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS
Sbjct: 265 DDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 324

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 362
           PWLLSVAASGMDRKFVTRVQLGNGNIY+GISINTF+MNLQ+PLVYAGDIPNLGFNGSTSR
Sbjct: 325 PWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLVYAGDIPNLGFNGSTSR 384

Query: 363 YCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPN 422
           YCE+NSVNP LAAGKILLCDSVIP S F SFGAI GILMQAYTRDNARSYPAPAAVL PN
Sbjct: 385 YCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIVGILMQAYTRDNARSYPAPAAVLDPN 444

Query: 423 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEI 482
           DGIAVYRYIRSTIIPTATIFKSVAGQDSSAP+VVSFSSRGPNALTNDILKPDLSGPGVEI
Sbjct: 445 DGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEI 504

Query: 483 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITT 542
           LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSAL+TT
Sbjct: 505 LAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTT 564

Query: 543 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 602
           ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGY T+MVRRITGD
Sbjct: 565 ASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYDTAMVRRITGD 624

Query: 603 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 662
           NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI
Sbjct: 625 NSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSI 684

Query: 663 TVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           TVNPTALSFNGIGDRKSFRLTV GTVSQSISSASLEWSDGVHSVRSPITVVALTK
Sbjct: 685 TVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDGVHSVRSPITVVALTK 739

BLAST of Cp4.1LG01g10970 vs. ExPASy TrEMBL
Match: A0A6J1H1P2 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1)

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 574/712 (80.62%), Postives = 631/712 (88.62%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           VLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRAMLEQVVGSTFDPQSVLYTY RSFN
Sbjct: 18  VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFN 77

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFPQNV RVPQLESNIVVGV
Sbjct: 78  GFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVDRVPQLESNIVVGV 137

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGRAYRIGRPILPGDVGSPR 182
           LDSGIWPESPSFNDDGFGSPPSKWRG CQVSNF CNRK+IG RAY IG+ I P +V SPR
Sbjct: 138 LDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGARAYHIGKSIAPDEVNSPR 197

Query: 183 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 242
           DT+GHGTHTAS VAGGLVSQASLY LG+GTARGG PSARIA YK+CWK GCSDADILAAF
Sbjct: 198 DTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKICWKHGCSDADILAAF 257

Query: 243 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 302
           DDAIADGVD +SLSVGSS+PRPYF+DPIAIGSFHA+K GILTSNSAGNEGPKD TTTSLS
Sbjct: 258 DDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNSAGNEGPKDSTTTSLS 317

Query: 303 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFD-MNLQYPLVYAGDIPNLGFNGSTS 362
           PWLLSVAA+ MDRKFVTRV+LG+G  ++G+SINTF+ M  QYPLVY GD+PN GF  S S
Sbjct: 318 PWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLVYGGDVPNRGFKRSIS 377

Query: 363 RYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 422
           RYC KNSVNP    GK+LLC++++P S+FSS GAI G++MQ+ TRDNA SYP PAA++  
Sbjct: 378 RYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTRDNAGSYPLPAALVSR 437

Query: 423 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 482
            D I ++R+ R T+ P A  FKS A QD SAP++ SFSSRGPN+L N ILKPDLSGPGVE
Sbjct: 438 QDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLANHILKPDLSGPGVE 497

Query: 483 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 542
           IL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVYVKTFNPTLSPAAIKSAL+T
Sbjct: 498 ILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMT 557

Query: 543 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 602
           TASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++YV FLCGQGY T+MVRRITG
Sbjct: 558 TASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITG 617

Query: 603 DNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQYFTRTLTNVARGGSTYRAKISAPQGL 662
           DNSACTSGNIGRVWDLN PSFA+S++ G++ +NQYFTRTLTNVA  GSTYRA ISAP+GL
Sbjct: 618 DNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGL 677

Query: 663 SITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITV 712
            ITVNP  LSFN IG+RKSF LTVGGTVS+ + SA L WSDGVHSVRSPIT+
Sbjct: 678 RITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGVHSVRSPITI 729

BLAST of Cp4.1LG01g10970 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 560/716 (78.21%), Postives = 631/716 (88.13%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           +LAS   S  D RKIYIVYMG+KLEDP SAHLHHRAMLE+VVGSTF P+SV+YTY RSFN
Sbjct: 23  LLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGSTFAPESVIYTYKRSFN 82

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GF V+LTEEEAQ+IA+KEGVVSVFLNQ NH+HTT SWDFLGFPQ V R+ Q+ESNIVVGV
Sbjct: 83  GFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQAVARMRQVESNIVVGV 142

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFRCNRKLIGGRAYRIGRPILPGDVGSP 182
           LDSGIWPESPSF D+GFG  PS W+G+CQ S NF CN K+IG RAYR+G  + PGDV SP
Sbjct: 143 LDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNNKIIGARAYRVGGTLPPGDVSSP 202

Query: 183 RDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAA 242
           RDTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAYK+CW DGCSDADILAA
Sbjct: 203 RDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKICWSDGCSDADILAA 262

Query: 243 FDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSL 302
           FDDAIADGV I+SLSVG S+ RPYFNDPIAIG+FHA+K+GILTSNSAGNEG K FTTTSL
Sbjct: 263 FDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILTSNSAGNEGSKFFTTTSL 322

Query: 303 SPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTS 362
           SPWLLSVAAS  DRKFVT VQLGNG IY+G +INTFDMN QYPLVYAG++PN+GFNGSTS
Sbjct: 323 SPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDMNNQYPLVYAGEVPNVGFNGSTS 382

Query: 363 RYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 422
           R+C  NSVNP L  GKILLCDS++PSS+FSSFGAIAG+LMQ YT DNARS+  PA VLHP
Sbjct: 383 RFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQGYT-DNARSHSVPATVLHP 442

Query: 423 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 482
            DG  VYRYI ST  PTA I      +D+SAP+V+SFSSRGPNALTND+LKPD++GPGVE
Sbjct: 443 QDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPNALTNDLLKPDITGPGVE 502

Query: 483 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 542
           ILAAWPP+API+GV++SRS+L+NIISGTSMSCPHL+ VAVYVKTFNP+ SPAAIKSAL+T
Sbjct: 503 ILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVKTFNPSWSPAAIKSALMT 562

Query: 543 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 602
           TASPM+AR +S+AEFAYG+GHVNPLKA+NPGLVYDA++SDYV FLCGQGY+T+M+RRITG
Sbjct: 563 TASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVKFLCGQGYNTAMLRRITG 622

Query: 603 DNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLS 662
           DNSACTS NIGRVWDLNYPSFA+++SG +++NQYFTRTLTNVA   STYRA IS PQGL+
Sbjct: 623 DNSACTSANIGRVWDLNYPSFALAVSGYQSLNQYFTRTLTNVASQASTYRATISVPQGLT 682

Query: 663 ITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTK 717
           ITVNP  LSF+G GDRKSF LTV G+VSQ I S SL WSDGVHSVRSP+ VV LTK
Sbjct: 683 ITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGVHSVRSPLVVVVLTK 737

BLAST of Cp4.1LG01g10970 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 534/714 (74.79%), Postives = 612/714 (85.71%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFN 62
           +L S+S S+ D RKIYIVYMGSKLED +SAHL+HRAMLE+VVGSTF P+SV+YTY RSFN
Sbjct: 22  LLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGSTFAPESVIYTYKRSFN 81

Query: 63  GFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGV 122
           GF V+LTEEEA +IA+KEGVVSVF ++ NHLHTTRSWDFLG  QNV RV Q+ESNIVVGV
Sbjct: 82  GFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQNVPRVKQVESNIVVGV 141

Query: 123 LDSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFRCNRKLIGGRAYRIGRPILPGDVGSP 182
            DSGIWPE+PSFNDDGFG  P+ WRG+CQ S NFRCNRK+IG RAYR    + PGDV SP
Sbjct: 142 FDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARAYRSST-LPPGDVRSP 201

Query: 183 RDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAA 242
           RDTDGHGTHTASTVAG LVSQASLYGLG+GTARGGVP ARIA YK+CW DGCSDADILAA
Sbjct: 202 RDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYKICWSDGCSDADILAA 261

Query: 243 FDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSL 302
           FDDAIADGVDI+SLSVG   P+PY  + IAIGSFHA+K GILTSNSAGN GPK FT TSL
Sbjct: 262 FDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTSNSAGNNGPKSFTVTSL 321

Query: 303 SPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTS 362
           SPWL +VAAS  DRKFVT+V LGNGN Y+G+SINTFDM  QYPL+YAG+ P++GFN STS
Sbjct: 322 SPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMRNQYPLIYAGNAPSIGFNSSTS 381

Query: 363 RYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 422
           RYC ++SV+P+L  GKILLCDS    ++F+SFG  AG+LMQ+ TRD+A SYP PA+VL P
Sbjct: 382 RYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQSNTRDHASSYPLPASVLDP 441

Query: 423 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 482
             G  + RY+ ST  PTATIFKS   +D+SAP+VVSFSSRGPN +T+DILKPD + PGVE
Sbjct: 442 AGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNYVTHDILKPDSTAPGVE 501

Query: 483 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 542
           ILAAWPPVAPI+GV+DSRS+LYNIISGTSMSCPH+T +AV++KTF P+ SPAAIKSAL+T
Sbjct: 502 ILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAVHIKTFYPSWSPAAIKSALMT 561

Query: 543 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 602
           TASPM+AR +S AEFAYG+GHVNPLKA++PGLVYDA+ESDYV FLCG+GY+T+MVR  TG
Sbjct: 562 TASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESDYVKFLCGEGYTTAMVRSTTG 621

Query: 603 DNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLS 662
           DNSACTSGNIGRVWDLNYPSFA+SIS ++T NQ F RTLTNV  G STYRA ISAPQGLS
Sbjct: 622 DNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTLTNVVSGASTYRASISAPQGLS 681

Query: 663 ITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVAL 715
           I+VNP+ LSFNGIGD+KSF LTV GTVSQ+I SASL WSDG H+VRSPITV  L
Sbjct: 682 ISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWSDGSHNVRSPITVYVL 734

BLAST of Cp4.1LG01g10970 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 1030 bits (2663), Expect = 0.0
Identity = 515/714 (72.13%), Postives = 589/714 (82.49%), Query Frame = 0

Query: 4   LASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFNG 63
           LAS   S  D + IYIVYMG KLEDP SAHLHHRAMLEQVVGSTF P+SVL+TY RSFNG
Sbjct: 20  LASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNG 79

Query: 64  FVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNVGRVPQLESNIVVGVL 123
           F V+LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDFLGFP  V R  Q+ESNIVVGVL
Sbjct: 80  FAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVL 139

Query: 124 DSGIWPESPSFNDDGFGSPPSKWRGSCQVSN-FRCNRKLIGGRAYRIGRPILPGDVGSPR 183
           D+GIWPESPSF+D+GF  PP KW+G+C+ SN FRCNRK+IG R+Y IGRPI PGDV  PR
Sbjct: 140 DTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPR 199

Query: 184 DTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAF 243
           DT+GHGTHTAST AGGLVSQA+LYGLG+GTARGGVP ARIAAYKVCW DGCSDADILAA+
Sbjct: 200 DTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADILAAY 259

Query: 244 DDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLS 303
           DDAIADGVDI+SLSVG + PR YF D IAIGSFHA++ GILTSNSAGN GP  FTT SLS
Sbjct: 260 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 319

Query: 304 PWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPLVYAGDIPNLGFNGSTSR 363
           PWLLSVAAS MDRKFVT+VQ+GNG  ++G+SINTFD N  YPLV   DIPN GF+ STSR
Sbjct: 320 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYYPLVSGRDIPNPGFDKSTSR 379

Query: 364 YCEKNSVNPSLAAGKILLCDSVI-PSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHP 423
           +C  NSV P L  GKI++C++   P   F S    AG+LM + TRD A SYP P++VL P
Sbjct: 380 FCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDP 439

Query: 424 NDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVE 483
           ND +A  RYI S   P ATIFKS    ++SAP+VVSFSSRGPN  T D++KPD+SGPGVE
Sbjct: 440 NDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVE 499

Query: 484 ILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALIT 543
           ILAAWP VAP+ G++  R++L+NIISGTSMSCPH+TG+A YVKT+NPT SPAAIKSAL+T
Sbjct: 500 ILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMT 559

Query: 544 TASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITG 603
           TASPM+AR + QAEFAYG+GHVNPLKA+ PGLVYDANESDYV FLCGQGY+T  VRRITG
Sbjct: 560 TASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITG 619

Query: 604 DNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLS 663
           D SACT GN GRVWDLNYPSF +S+S ++T NQYF RTLT+VA   STYRA ISAPQGL+
Sbjct: 620 DYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLT 679

Query: 664 ITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVAL 715
           I+VNP  LSFNG+GDRKSF LTV G++   + SASL WSDGVHSVRSPIT+ +L
Sbjct: 680 ISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHSVRSPITITSL 730

BLAST of Cp4.1LG01g10970 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 608.2 bits (1567), Expect = 1.0e-173
Identity = 333/715 (46.57%), Postives = 459/715 (64.20%), Query Frame = 0

Query: 13  DDRKIYIVYMGSKLE----DPSSAHLHHRAMLEQVVGSTFDPQSVLYTYTRSFNGFVVRL 72
           DD+++YIVYMGS        P+S H++   +L++V G +     ++ +Y RSFNGF  RL
Sbjct: 27  DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQEVTGESSIEGRLVRSYKRSFNGFAARL 86

Query: 73  TEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQNV--GRVPQLESNIVVGVLDSG 132
           TE E + +A   GVVSVF N+   L TT SWDF+G  + +   R P +ES+ ++GV+DSG
Sbjct: 87  TESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSG 146

Query: 133 IWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILPGDVGSPRDTD 192
           I PES SF+D GFG PP KW+G C    NF CN KLIG R Y              RD D
Sbjct: 147 ITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY---------TSEGTRDMD 206

Query: 193 GHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSDADILAAFDDA 252
           GHGTHTAST AG  V  AS +G+G GT RGGVP++R+AAYKVC   GCS   +L+AFDDA
Sbjct: 207 GHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDA 266

Query: 253 IADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPWL 312
           IADGVD++++S+G      + NDPIAIG+FHA+  G+LT NSAGN GPK  + + ++PW+
Sbjct: 267 IADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWI 326

Query: 313 LSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNLGFNGSTSRYC 372
           L+VAAS  +R FVT+V LGNG    G S+N ++M    YPLVY     +   +  ++  C
Sbjct: 327 LTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLC 386

Query: 373 EKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPNDG 432
           E + V+ S   GKIL+C       I  S GA+ G++ +    D A  +P PAA L   D 
Sbjct: 387 ELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GLIYRTPKPDVAFIHPLPAAGLLTEDF 446

Query: 433 IAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEILA 492
            ++  Y+ ST  P A + K+ A  + ++P++ SFSSRGPN +  DILKPD++ PGVEILA
Sbjct: 447 ESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILA 506

Query: 493 AWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTAS 552
           A+ P A      D+R   Y+++SGTSMSCPH+ GVA YVKTFNP  SP+ I+SA++TTA 
Sbjct: 507 AYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAW 566

Query: 553 PMSARQH--SQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGD 612
           P++A     +  EFAYG+GHV+P+ A NPGLVY+ ++SD+++FLCG  Y++ +++ I+G+
Sbjct: 567 PVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGE 626

Query: 613 NSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYRAKISAPQG-- 672
              C+        +LNYPS +  +SG+  T    F RTLTNV    STY +K+ A  G  
Sbjct: 627 TVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSK 686

Query: 673 LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVRSPITV 713
           L + + P+ LSF  + +++SF +TV G+   S+  SSA+L WSDG H+VRSPI V
Sbjct: 687 LDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVV 727

BLAST of Cp4.1LG01g10970 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 1.3e-173
Identity = 340/717 (47.42%), Postives = 459/717 (64.02%), Query Frame = 0

Query: 14  DRKIYIVYMGSKLEDPSSAHL----HHRAMLEQVVGSTFDPQSVLYTYTRSFNGFVVRLT 73
           D+++YIVYMG+    PS        HH ++L+ V G +     ++  Y RSFNGF  RLT
Sbjct: 30  DKQVYIVYMGAL---PSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLT 89

Query: 74  EEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLESNIVVGVLDSGI 133
           E E + +AS + VVSVF ++N +L TT SW+F+G  +     R P +ES+ ++GV+DSGI
Sbjct: 90  ESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGI 149

Query: 134 WPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILPGDVGSPRDTDG 193
           +PES SF+  GFG PP KW+G C+  +NF CN KLIG R Y    P L G   S RD  G
Sbjct: 150 YPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY---TPKLEGFPESARDNTG 209

Query: 194 HGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKD--GCSDADILAAFDD 253
           HG+HTAS  AG  V   S YGLG GT RGGVP+ARIA YKVC      C+   ILAAFDD
Sbjct: 210 HGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDD 269

Query: 254 AIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPW 313
           AIAD VDI+++S+G+     +  D +AIG+FHA+  GILT N AGN GP+  T  S++PW
Sbjct: 270 AIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPW 329

Query: 314 LLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMN-LQYPLVYAGDIPNLGFNGSTSRY 373
           L +VAAS M+R F+T+V LGNG    G S+N+FD+N  +YPLVY G   +   + S++ +
Sbjct: 330 LFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY-GKSASSRCDASSAGF 389

Query: 374 CEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPAPAAVLHPND 433
           C    ++     GKI+LCD+        + GA+A I+   Y  D A  +  P +VL  +D
Sbjct: 390 CSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPY-EDAASVFSFPVSVLSEDD 449

Query: 434 GIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPDLSGPGVEIL 493
              V  Y+ ST  P A + KS    +  AP+V S+SSRGPN L +DILKPD++ PG EIL
Sbjct: 450 YNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEIL 509

Query: 494 AAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTA 553
           AA+ P  P     D+R   Y +ISGTSMSCPH+ GVA Y+KTF+P  SP+ I+SA++TTA
Sbjct: 510 AAYSPYVP-PSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTA 569

Query: 554 SPMSAR---QHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRIT 613
            PM+A     +  AEFAYGAGHV+P+ AI+PGLVY+AN+SD+++FLCG  Y+   +R I+
Sbjct: 570 WPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLIS 629

Query: 614 GDNSACTSGNIGRVW-DLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQG 673
           GD+S+CT      +  +LNYPS +  +SGT+     F RT+TNV R  +TY+AK+   + 
Sbjct: 630 GDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK- 689

Query: 674 LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVRSPITVVA 715
           L + V P  LS   + ++KSF +TV G    ++++ SA L WSDGVH VRSPI V A
Sbjct: 690 LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Cp4.1LG01g10970 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 606.3 bits (1562), Expect = 3.9e-173
Identity = 341/725 (47.03%), Postives = 461/725 (63.59%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLED----PSSAHLHHRAMLEQVVGSTFDPQSVLYTYT 62
           +L+S S    +D ++YIVYMGS        P+S H+   ++L+QV G +     ++ +Y 
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIEGRLVRSYK 77

Query: 63  RSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLES 122
           RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDF+G  +  N  R   +ES
Sbjct: 78  RSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIES 137

Query: 123 NIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILP 182
           + ++GV+D+GIWPES SF+D GFG PP KW+G C    NF CN KLIG R Y        
Sbjct: 138 DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY-------- 197

Query: 183 GDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSD 242
                 RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKVC   GCS 
Sbjct: 198 -TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSS 257

Query: 243 ADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKD 302
             +L++FDDAIADGVD++++S+G   P  + +DPIAIG+FHA+  GILT +SAGN GPK 
Sbjct: 258 EALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKP 317

Query: 303 FTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNL 362
            T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N FDM   +YPLVY     + 
Sbjct: 318 TTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASS 377

Query: 363 GFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPA 422
             +  T+  C    +N S   GKIL+C       I  S GAIA I+ ++   D A ++  
Sbjct: 378 ACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHL 437

Query: 423 PAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPD 482
           PA+ L   D  ++  YI S   P A + K+    + ++P++ SFSSRGPN +  DILKPD
Sbjct: 438 PASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPD 497

Query: 483 LSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAA 542
           ++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF P  SP+ 
Sbjct: 498 ITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 557

Query: 543 IKSALITTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYS 602
           I+SA++TTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++FLCG  Y+
Sbjct: 558 IQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 617

Query: 603 TSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYR 662
           +  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV    STY+
Sbjct: 618 SKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYK 677

Query: 663 AKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVR 713
           +K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L WSDG H+VR
Sbjct: 678 SKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVR 728

BLAST of Cp4.1LG01g10970 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 602.8 bits (1553), Expect = 4.3e-172
Identity = 339/723 (46.89%), Postives = 458/723 (63.35%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLED----PSSAHLHHRAMLEQVVGSTFDPQSVLYTYT 62
           +L+S S    +D ++YIVYMGS        P+S H+   ++L+QV G +     ++ +Y 
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIEGRLVRSYK 77

Query: 63  RSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLES 122
           RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDF+G  +  N  R   +ES
Sbjct: 78  RSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIES 137

Query: 123 NIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILP 182
           + ++GV+D+GIWPES SF+D GFG PP KW+G C    NF CN KLIG R Y        
Sbjct: 138 DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY-------- 197

Query: 183 GDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSD 242
                 RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKVC   GCS 
Sbjct: 198 -TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSS 257

Query: 243 ADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKD 302
             +L++FDDAIADGVD++++S+G   P  + +DPIAIG+FHA+  GILT +SAGN GPK 
Sbjct: 258 EALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKP 317

Query: 303 FTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNL 362
            T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N FDM   +YPLVY     + 
Sbjct: 318 TTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASS 377

Query: 363 GFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPA 422
             +  T+  C    +N S   GKIL+C       I  S GAIA I+ ++   D A ++  
Sbjct: 378 ACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHL 437

Query: 423 PAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPD 482
           PA+ L   D  ++  YI S   P A + K+    + ++P++ SFSSRGPN +  DILKPD
Sbjct: 438 PASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPD 497

Query: 483 LSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAA 542
           ++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF P  SP+ 
Sbjct: 498 ITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 557

Query: 543 IKSALITTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTS 602
           I+SA++TTA     R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++FLCG  Y++ 
Sbjct: 558 IQSAIMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSK 617

Query: 603 MVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYRAK 662
            ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV    STY++K
Sbjct: 618 TLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSK 677

Query: 663 ISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVRSP 713
           + A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L WSDG H+VRSP
Sbjct: 678 VVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSP 723

BLAST of Cp4.1LG01g10970 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 602.4 bits (1552), Expect = 5.6e-172
Identity = 341/725 (47.03%), Postives = 461/725 (63.59%), Query Frame = 0

Query: 3   VLASTSHSHGDDRKIYIVYMGSKLED----PSSAHLHHRAMLEQVVGSTFDPQSVLYTYT 62
           +L+S S    +D ++YIVYMGS        P+S H+   ++L+QV G +     ++ +Y 
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIEGRLVRSYK 77

Query: 63  RSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFPQ--NVGRVPQLES 122
           RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDF+G  +  N  R   +ES
Sbjct: 78  RSFNGFAARLTESERTLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIES 137

Query: 123 NIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQ-VSNFRCNRKLIGGRAYRIGRPILP 182
           + ++GV+D+GIWPES SF+D GFG PP KW+G C    NF CN KLIG R Y        
Sbjct: 138 DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY-------- 197

Query: 183 GDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAYKVCWKDGCSD 242
                 RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKVC   GCS 
Sbjct: 198 -TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSS 257

Query: 243 ADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTSNSAGNEGPKD 302
             +L++FDDAIADGVD++++S+G   P  + +DPIAIG+FHA+  GILT +SAGN GPK 
Sbjct: 258 EALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKP 317

Query: 303 FTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDM-NLQYPLVYAGDIPNL 362
            T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N FDM   +YPLVY     + 
Sbjct: 318 TTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASS 377

Query: 363 GFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYTRDNARSYPA 422
             +  T+  C    +N S   GKIL+C       I  S GAIA I+ ++   D A ++  
Sbjct: 378 ACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHL 437

Query: 423 PAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNALTNDILKPD 482
           PA+ L   D  ++  YI S   P A + K+    + ++P++ SFSSRGPN +  DILKPD
Sbjct: 438 PASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPD 497

Query: 483 LSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAA 542
           ++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF P  SP+ 
Sbjct: 498 ITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 557

Query: 543 IKSALITTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYS 602
           I+SA++TTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++FLCG  Y+
Sbjct: 558 IQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 617

Query: 603 TSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVARGGSTYR 662
           +  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV    STY+
Sbjct: 618 SKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYK 677

Query: 663 AKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSDGVHSVR 713
           +K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L WSDG H+VR
Sbjct: 678 SKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVR 726