Cp4.1LG01g10000 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g10000
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProlyl endopeptidase
LocationCp4.1LG01: 6919461 .. 6925752 (-)
RNA-Seq ExpressionCp4.1LG01g10000
SyntenyCp4.1LG01g10000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGAAAACATTGTTGACTATATGAAGCCACTTCAACAAAATGTCATATTTGGTTTGGTTAGAAGAAGCGCTATTCTCTTCATTCCTTTTGCCGGCTCTCCGTCGGTAGCTTCATTCCGTCACTTCCAATCGCCGGCTTCATCGACGGTTGTCACAATGAGCTATTCTCACTCTCCTCCGGTGGCTAAGAAGGTGGAACACAAAATGGAGTTGTTTGGGGATGTAAGGATCGACAATTATTACTGGTTGCGCGACGATTCTCGCAAAAATGCCGATATCATTTCGTATTTGCAGCAGGAGAATGCCTATACTGATTTCGTCATGTCCGGTGAACTATAGTTTACATTTCGTCTTGGTTCTTGAATTTTTACTGGTAGTTTATAAGAAATTTATGAATCAGTGATATGGTAGCATTGAATAGGAGTATTGTCATTTGATCTATCTTTTGATTTCTGATGATTTGTATCTAATGAGGATATTTCGATGAATGTGTGCGTTTTTCTTGATTCATCGATCTAGGAACAAAGCAAGTGGAAGAACAGATTTATGGTGAGATAAGAGGACGAATCAAGGAGGATGATATATCTGTGCCTGAGAGAAAAGGCCCTTACTATTACTATAAGAGGACTCTAGACGGGAAAGAATATGTTCAATATTGCAGGCGTTTTGTACCCCCTGGTGAAGAGGCTGTCTCTGTGCACGATGTCATGCCTACTGGACCTGGTGCTCCTCCGGAGCATGTTATATTGGACGAAAATGACAAGGCACAGAACCAATCCTACTACTGCATTGGTGCCTTTGAGGTATTGTAGGCGTGGATCTGCTTCCGAATTTGATCCTGTTTCTCTTAGACGAATTTTTAATTTAAGGATGTTTACATTACGGCCATTTCTTCTCTAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTCTATGTTATTGATGCCGAGACTGGAGCTCCTGTAGGAAAACCTCTTGCAGGCGTGACATCGTATCTCAAATGGGCTGGCAATGACGCTTTAGTATACATCACAATGGATGAGATTCTTCGACCTGATAAGGTGATCTTCTATATTTTTCATCGATTTTACCGTGGTTCTCTCATTGGAAAAGCACTAACAATCATTTAGTTCCTGAGGCCTATCATACCCAATATCCTTGTTGATTGTTCTGTGCATTCAGAATCGACTTCTAATCAAGTAGTTGGTAAATTCGTTAAAAAGAAGATGTCCTCTATACCATTAGAATAGCTTGCACTTCCTCTAGTTTCTCTTTTGTAATATTGATATAGATTATAATATCCTAATAAGTTGATATCAGAAGAGTTTTTATAGTTGTAATGAGCACTGCTCAGAGTACATATGTTGGGTGAAAATACATTCAGTATCTGCATTCTGATGTTCCCATAAATATTATGGTCAAACTTCAAATTAAAAAATTTATTTTAGCGTGTACAGAATTTCAGATGTTGATCTAGTAAATTTTCTCTCCTTTCAATAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAACGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTCGATCTTCAAGCTTCTGAGAGCAAGAAATATTTGTTTATTGCATCTGAAAGTAAGTTTACTAGGTTCAACTTTTACCTTGATGTATCAAGGCCAGAAGATGGGCTTACTGTCTTGACACCTAGAGTGGATGGAGTTGACACTTTTCCCAGTCATCGTGGAAATCATTTTTTTATCCGGAGACGAAGTGAAGAGATTTTCAATTCAGAAGTAGTAGCTTGCCCTCTCGATAATATATCAGCGACAATGGTTCTTCTTCCGCACAGGGAAAGGTAGTGATTTGTATTCTCTTATGATACTTTATGATGGACCTGATGGTATAATGTGAATTGAAACAAAATTGGAAGTGATGAGCTGATAGTTAATGACTTGAATCAACTAGCCTGAAAATATATTTTGTATGAACTTGCTGTTTATTTATTCTATTTGTTGGAAATCTGTTAGGGCAGCTATTTTTATAAAGTGAAAGACCCAACTGATCTGTATCAACTATAATTAGGATTTTCATGTTCACTTGGTCTGACACTTGGTACTTATTTCGATTTAGGTTCAACACAAAAATGAGCTCCTTATTGTGCTGTGAATTTGATTTTGTCTATTAAAACTGTTGAAAGCTTCTTAATTAGCTTATGTTTCAATATCTCTCTCTATTATTTCCTGCAACCAGTCGAGGTAACGAGCTTGTCATTGGGGTTTATTAAGTTGTTTGAATACTGTCCAGTGTGAAAATCCAGGACATACAACTGTTTCTTAACCACATCGTTGTATTCGAACGTGAAGATGGTCTACCAAAAATTGTTGTCTATAGCCTTCCTGATATTGGAGAACCACTTAGAAGCCTTGAAGGTGGACGAGCTGTGGATTTTACTGATGCGACTTATTCAGTGGCTTTATCAGAATCAGAATTCTCTTCCAGTATTTTACGGTTCTGTTACAGCTCAATGAGGACACCCCCCTCTACATACGACTATGATATGAAATCAGGAGTTTCCATTCTAAAGAAAGTTGAAACAGTGAGTAGTTCCGCAATCATGGTTATGTTGTTTTTTCTTATGACGGGGATTGCCTCTAGGTTTAAATGATTGTCAAAAGAAAAATATCTATCTATTTATCTATCTGTCTATATATATATAAACCAGATACAAATTTCTATCGTCTGTTTTTTTCAATCCACTAACTACTCATTTGAGGCAAGGAAAATATGTTATTTATCTAGTCTTTCTAAAACCATTGCAAATTTCTTCAACTTTCTGTACATTCTTTTACTCTCTTTTTTTCACTTTTTATACTTAAGATTGTTATTCCCTTATGTAAATAATTTTGTAATATTTTACAGAAATCTTCTCTGTCTCTTTGTTGAAGTCATGCCTTTTCACTATCCACTTTTTTATACTTGAGAATTCCTGTTGTCTCGCAAATCCAGAAATCTTAGTTTTCCCTCCCCCATCTGGGGATTCGAATTTATTTATTTGTCTGTAACATAGTGATATTTTCACAACTTCTATCAGGTGTTGGGAGGTTTTGATACTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTCTAGATGGCACTAAAGTTCCCCTATCAATTGTTTATCGAAAGGATCTAGTGAAACTTGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTACGAGGTAAAAAGACCCGTGTTAATCAGATTCTAGACAGATTTCAGGATTTTGAAGTTATTTTAAAGTGAGTGTTTTTGTTTCCTGGGGCCTCCCCCTGTGAATCAGAGTTTTGTTGATGGAGCTTTAATTAATGTAAAGTTATTTTCCTATGTTAATTGATTTATCAAAATTTTCAATTCGGAAATATTTATTTATGCATTGTAATACATTCTTTGACGATCTATGTTCCATTCTGATTTCCTAGTTTTTGCTATAATTATGATCTCTTCCAGTTGCCAATTTACCGATCTTAATTATTTGCATAGGACATGACCTTGTACCTGAATCTTTCCGTGTACTTTCTTCTTGTTACTTAACAGTGTTATGAATTGAAACAATGATTGTACATGGACATGGTCTTGATATGAATTTCATTCTTTGAACAGATATGCATAGACCCTAGTTTCAAGGGATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATACAATAGCTCACATTCGTGGAGGTGGTGAAATGGGGAGGCAGTGGTACGAAAATGGAAAGTTACTGAAGAAAAAGAATACATTCACGGATTTTATTTCTTGTGCTGAATACTTGATCGAGAATAAATACTGTTCAAGGGAAAAACTGTGCATTGATGGAAGAAGTGCTGGGGGTTTGCTTATTGGTGCTGTTCTAAATATGAGGCCTGATTTGTTCAAAGCTGCAGTTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCGACAATTCCCCTTACGACTTCGGAGTGGGAGGTAAATCTCATCCCTACTGTACCTTTCGTAATCCTGTTAGTAGTACTAAGCAGTGAGGTAGTCCAATTCCTCGGTCGGAGTGAGGTAGTCCAATTCCTCGGTCGGTACATAAATAGACCAATTAAGGAGGACTCTTAAGGTTTAGCTTATGCTAGAAATGGCTTAAAATCACTTCCGGGTCCCCAAATTATTGTGCTATTTTGAACGAGAGTCCAATGTAGGTAAATATATATTAAAAAGACAAAAGGGTGGAAAATGTTTCTGTTTATAGCACGCACATAGAAAGCCATTCCCAAATACGTTCATATACTTGCTACAATGCTTTCGTTACATTTCAACTTTGGTTCTTAACATACGAAATTCGCTTCGCGTGCGTAAATATTTCTACTTATCCATTATCATTTCTTTTAATTTTTCCCACACTTTTCAACTTTCGTTTACTGAAAATATGTTATTTAATTTATTGCAGCGCCTATTCTTTTTGACCTGCTTCTTACCGTCTTTTGTATTCTGCAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCGCCAGTTGACAATGTATGCACNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATTTTATATATATCGACATTCTGTTTCGGGATTTCCACCTAATCATCGACTTGATTTGAACTTGATTTTGGATCATTCGAATGTATCGGTGCTTATTTGTTTCACAGAATATTTTGAAACTTTTGTTTTCACTAGGACATGACTTTATCTGCATTTTTTCAGATTAAGGCTCGACATTATCCAGACATACTTGTTACCACCGGCTTAAACGGTAAGTGTGAATGCTTAAAACTTCACTCCAAGTAGAGCCAATCAATTTCCCTGTTTATTCATCCTGTGCACACAATCTCCCTTCATATTCAGAATGGACAAGGAGATTGATGAATATATCTATATTTTTTTAGAGACAAATCTTCAAAGTGTCAATTCCTTTTGTCCATGTGGCAGATCCCCGTGTTTTATATTCAGAACCCGCTAAGTTTGTGGCAAAATTAAGGGATATGAAGACCGATGATAATCTTTTGCTTTTCAAATGTGAACTTGGTGCCGGACACTTTTCAAAGTCGGGAAGGTAATTATTCTATGGTTGATTTCTTTTGTAGGCCTTACTTCATGATCTGTGTTCTCTTGCTTGTTTTTATCTCATCTCTTTTTGTTGCTTGTGCCTTTTTTCTTCTTTAATATGATAGTAAAATTATTCTTACTTAATTGATGACATTAACTGCTGCCTTTTATTTATTTATTTATTTACAACTCTACTGTAGATATGGAGAAGAAACACTATGTTGAACTCTGTTATGATTTGTCACTGCCTATTTAAAAAACTGTAATGAGGAGAATTATAACATTTAAATCATTGTTTATTTGTTCATAAGCCTGTAACTATTGAAGTAGAACTCCAATTTTCTTTTATTTATTATTATTTTTTTTAGTGTAGGACCTTTATGTGGGTATAGAAAATCCAGATTTTTAGTTACTTCGTCTTCTCTTGGATTTGAGTCATTTTCTTAATGAGTGTGGAACTCCTTTTTGTGGAGTGCTTTTTTGCATGTCTATCCATGAAAGTCTGATCTCTCATAATTCTCTTTTTTTTTTTTCAAACATAGGCCACCCGTGTTAGTAAACTTGGAAAGATATAAAAAGGAAAACAACCTTGAGAATACAAACTGAACATCATGGCATTAATCTTTTGTTATTAAAAAAGGGAAAACACGCTTACATTTTGAAGTTTTTCATNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCTGCCATGCAGATTTGAGAAGCTACAAGAGGATGCTTTCACGTACGCTTTTATACTGAAGTCACTTAACATGGTTCCTGCTCTCGGAAATTGAGCAGAAAAGCCGCCCTTGATGGATAACAAGTACCATCTCTAAACTTTCATTCTCTATTCATCTTTCTAGTGAATCTTCCTTTATGAGAAATAGGGGCCATATTTGTACTGTTCTTCATTACCTTAGCATTAGTTAAGCTATTATGCTATCTTAAGGTTACTAGTTAACACTCGTGTGCATTTGCACAACATTTTAAGAGATTTGAAATTAATCCAAACAAATTCACCCTCTCAATACCGGAGGGACTTTTATTCTTCCTTGCCACTCCAAAGAAAGATCTATTCTTTTTTATATTC

mRNA sequence

TGAGAAAACATTGTTGACTATATGAAGCCACTTCAACAAAATGTCATATTTGGTTTGGTTAGAAGAAGCGCTATTCTCTTCATTCCTTTTGCCGGCTCTCCGTCGGTAGCTTCATTCCGTCACTTCCAATCGCCGGCTTCATCGACGGTTGTCACAATGAGCTATTCTCACTCTCCTCCGGTGGCTAAGAAGGTGGAACACAAAATGGAGTTGTTTGGGGATGTAAGGATCGACAATTATTACTGGTTGCGCGACGATTCTCGCAAAAATGCCGATATCATTTCGTATTTGCAGCAGGAGAATGCCTATACTGATTTCGTCATGTCCGGAACAAAGCAAGTGGAAGAACAGATTTATGGTGAGATAAGAGGACGAATCAAGGAGGATGATATATCTGTGCCTGAGAGAAAAGGCCCTTACTATTACTATAAGAGGACTCTAGACGGGAAAGAATATGTTCAATATTGCAGGCGTTTTGTACCCCCTGGTGAAGAGGCTGTCTCTGTGCACGATGTCATGCCTACTGGACCTGGTGCTCCTCCGGAGCATGTTATATTGGACGAAAATGACAAGGCACAGAACCAATCCTACTACTGCATTGGTGCCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTCTATGTTATTGATGCCGAGACTGGAGCTCCTGTAGGAAAACCTCTTGCAGGCGTGACATCGTATCTCAAATGGGCTGGCAATGACGCTTTAGTATACATCACAATGGATGAGATTCTTCGACCTGATAAGGACATACAACTGTTTCTTAACCACATCGTTGTATTCGAACGTGAAGATGGTCTACCAAAAATTGTTGTCTATAGCCTTCCTGATATTGGAGAACCACTTAGAAGCCTTGAAGGTGGACGAGCTGTGGATTTTACTGATGCGACTTATTCAGTGGCTTTATCAGAATCAGAATTCTCTTCCAGTATTTTACGGTTCTGTTACAGCTCAATGAGGACACCCCCCTCTACATACGACTATGATATGAAATCAGGAGTTTCCATTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATACTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTCTAGATGGCACTAAAGTTCCCCTATCAATTGTTTATCGAAAGGATCTAGTGAAACTTGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTACGAGATATGCATAGACCCTAGTTTCAAGGGATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATACAATAGCTCACATTCGTGGAGGTGGTGAAATGGGGAGGCAGTGGTACGAAAATGGAAAGTTACTGAAGAAAAAGAATACATTCACGGATTTTATTTCTTGTGCTGAATACTTGATCGAGAATAAATACTGTTCAAGGGAAAAACTGTGCATTGATGGAAGAAGTGCTGGGGGTTTGCTTATTGGTGCTGTTCTAAATATGAGGCCTGATTTGTTCAAAGCTGCAGTTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCGACAATTCCCCTTACGACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCGCCAGTTGACAATATTAAGGCTCGACATTATCCAGACATACTTGTTACCACCGGCTTAAACGATCCCCGTGTTTTATATTCAGAACCCGCTAAGTTTGTGGCAAAATTAAGGGATATGAAGACCGATGATAATCTTTTGCTTTTCAAATGTGAACTTGGTGCCGGACACTTTTCAAAGTCGGGAAGATTTGAGAAGCTACAAGAGGATGCTTTCACGTACGCTTTTATACTGAAGTCACTTAACATGGTTCCTGCTCTCGGAAATTGAGCAGAAAAGCCGCCCTTGATGGATAACAAGTACCATCTCTAAACTTTCATTCTCTATTCATCTTTCTAGTGAATCTTCCTTTATGAGAAATAGGGGCCATATTTGTACTGTTCTTCATTACCTTAGCATTAGTTAAGCTATTATGCTATCTTAAGGTTACTAGTTAACACTCGTGTGCATTTGCACAACATTTTAAGAGATTTGAAATTAATCCAAACAAATTCACCCTCTCAATACCGGAGGGACTTTTATTCTTCCTTGCCACTCCAAAGAAAGATCTATTCTTTTTTATATTC

Coding sequence (CDS)

ATGAAGCCACTTCAACAAAATGTCATATTTGGTTTGGTTAGAAGAAGCGCTATTCTCTTCATTCCTTTTGCCGGCTCTCCGTCGGTAGCTTCATTCCGTCACTTCCAATCGCCGGCTTCATCGACGGTTGTCACAATGAGCTATTCTCACTCTCCTCCGGTGGCTAAGAAGGTGGAACACAAAATGGAGTTGTTTGGGGATGTAAGGATCGACAATTATTACTGGTTGCGCGACGATTCTCGCAAAAATGCCGATATCATTTCGTATTTGCAGCAGGAGAATGCCTATACTGATTTCGTCATGTCCGGAACAAAGCAAGTGGAAGAACAGATTTATGGTGAGATAAGAGGACGAATCAAGGAGGATGATATATCTGTGCCTGAGAGAAAAGGCCCTTACTATTACTATAAGAGGACTCTAGACGGGAAAGAATATGTTCAATATTGCAGGCGTTTTGTACCCCCTGGTGAAGAGGCTGTCTCTGTGCACGATGTCATGCCTACTGGACCTGGTGCTCCTCCGGAGCATGTTATATTGGACGAAAATGACAAGGCACAGAACCAATCCTACTACTGCATTGGTGCCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTCTATGTTATTGATGCCGAGACTGGAGCTCCTGTAGGAAAACCTCTTGCAGGCGTGACATCGTATCTCAAATGGGCTGGCAATGACGCTTTAGTATACATCACAATGGATGAGATTCTTCGACCTGATAAGGACATACAACTGTTTCTTAACCACATCGTTGTATTCGAACGTGAAGATGGTCTACCAAAAATTGTTGTCTATAGCCTTCCTGATATTGGAGAACCACTTAGAAGCCTTGAAGGTGGACGAGCTGTGGATTTTACTGATGCGACTTATTCAGTGGCTTTATCAGAATCAGAATTCTCTTCCAGTATTTTACGGTTCTGTTACAGCTCAATGAGGACACCCCCCTCTACATACGACTATGATATGAAATCAGGAGTTTCCATTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATACTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTCTAGATGGCACTAAAGTTCCCCTATCAATTGTTTATCGAAAGGATCTAGTGAAACTTGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTACGAGATATGCATAGACCCTAGTTTCAAGGGATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATACAATAGCTCACATTCGTGGAGGTGGTGAAATGGGGAGGCAGTGGTACGAAAATGGAAAGTTACTGAAGAAAAAGAATACATTCACGGATTTTATTTCTTGTGCTGAATACTTGATCGAGAATAAATACTGTTCAAGGGAAAAACTGTGCATTGATGGAAGAAGTGCTGGGGGTTTGCTTATTGGTGCTGTTCTAAATATGAGGCCTGATTTGTTCAAAGCTGCAGTTGCTGGGGTACCTTTTGTAGATGTTCTAACGACCATGCTTGATCCGACAATTCCCCTTACGACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCGCCAGTTGACAATATTAAGGCTCGACATTATCCAGACATACTTGTTACCACCGGCTTAAACGATCCCCGTGTTTTATATTCAGAACCCGCTAAGTTTGTGGCAAAATTAAGGGATATGAAGACCGATGATAATCTTTTGCTTTTCAAATGTGAACTTGGTGCCGGACACTTTTCAAAGTCGGGAAGATTTGAGAAGCTACAAGAGGATGCTTTCACGTACGCTTTTATACTGAAGTCACTTAACATGGTTCCTGCTCTCGGAAATTGA

Protein sequence

MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDKDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNIKARHYPDILVTTGLNDPRVLYSEPAKFVAKLRDMKTDDNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNMVPALGN
Homology
BLAST of Cp4.1LG01g10000 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 2.1e-125
Identity = 267/709 (37.66%), Postives = 386/709 (54.44%), Query Frame = 0

Query: 41  STVVTMSYSHS---PPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYT 100
           ST +T + + S   P VAKK        G  R D YYWLRDD R+N ++++YL  ENAYT
Sbjct: 15  STPITSALAASATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYT 74

Query: 101 DFVMSGTKQVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGE 160
           D VM+  K +E+++Y E+  RIK+DD SVP R+  ++YY R + GK+Y  + RR   PG 
Sbjct: 75  DAVMAPLKPLEDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPGV 134

Query: 161 EAVSVHDVMPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIY 220
           +AVS+      G  A  E V+LD N     + YY +G +EVS +N+L+AYA+DT G   Y
Sbjct: 135 DAVSIQAANAAGDFA-GEQVLLDVNALGAGKDYYNVGDYEVSQDNRLLAYADDTNGRRQY 194

Query: 221 TVYVIDAETGAPVGKPLAGVTSYLKW------------------------------AGND 280
           T+   + +TG  +   +      L W                              A  D
Sbjct: 195 TIRFKNLDTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQD 254

Query: 281 ALVYITMDE---------------------------------------ILRP-DKDIQLF 340
           ALVY   D+                                       +L P ++D++  
Sbjct: 255 ALVYEEEDDSFYMGIGRSRDDKFICISVESTVSSEMRCTPAASPGVFTVLAPRERDVEYQ 314

Query: 341 LNHI----VVFEREDGLPKIVVYSLPD-------------------------------IG 400
            +H+    V+    DG     + + P                                + 
Sbjct: 315 ADHLGDRWVIRTNADGATNFKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGFSVVA 374

Query: 401 EPLRSLEGGRAV------DFT---DATYSVALSES-EFSSSILRFCYSSMRTPPSTYDYD 460
           E   +LE  R +      D+    ++ YS+ LS + E  +  LR+ Y+SM TP +TY+ +
Sbjct: 375 ERANALESLRVIKADGSSDYVKADESAYSMGLSANPETGTDWLRYSYTSMTTPATTYEIN 434

Query: 461 MKSGVSILKKVETVLGGFDTNKYVTERKWATALDG-TKVPLSIVYRKDLVKLDGSDPLLL 520
            K+G     K + V  G+D +KYVTER WA A DG TK+P+++VYRKD+ + DG  P+L 
Sbjct: 435 TKTGERRQLKQQPV-PGYDASKYVTERVWAPARDGKTKIPVTLVYRKDVAR-DGKAPMLQ 494

Query: 521 YGYGSYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFI 580
           Y YGSY   +DP+F  + +SLLDRG +Y +AHIRGG EMGR WY++GKL  K NTFTDFI
Sbjct: 495 YAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMGRAWYDDGKLYNKINTFTDFI 554

Query: 581 SCAEYLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPT 631
              +YL++  Y +++++   G SAGGLL+GAV NM P+ +K  +  VPFVDV+TTMLDPT
Sbjct: 555 DVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVDVVTTMLDPT 614

BLAST of Cp4.1LG01g10000 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.9e-118
Identity = 250/691 (36.18%), Postives = 365/691 (52.82%), Query Frame = 0

Query: 53  PVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIY 112
           P+AK++ H  EL GDVR D+YYWL+D  R N ++I YL++EN Y   +M   ++  EQIY
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKD--RDNTEVIQYLEEENRYYHEIMRPLQEQTEQIY 63

Query: 113 GEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGA 172
             +  R+ + ++ VP + G ++YY R    K+Y  Y R+          + D        
Sbjct: 64  ESMVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARK---QAASRALLQDA------- 123

Query: 173 PPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGK 232
             E V+LD N+ A+   Y  +    ++ ++  +AY E+  G + YT+Y+ D  TG  +  
Sbjct: 124 -TEEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLSD 183

Query: 233 PLAGVTSY--LKWAG-NDALVYITMDEILRP----------------------DKDIQLF 292
            +  V  Y  ++W    D + Y T+DE  RP                      D    LF
Sbjct: 184 RVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTLF 243

Query: 293 LN--------------------HIV-----------VFEREDGLP--------------- 352
           ++                    H++           V ER DG+                
Sbjct: 244 ISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLILTN 303

Query: 353 ------KIVVYSLPDIGEPLRSLE-------------------GGR-------------- 412
                 +++   L D+   +  +E                    GR              
Sbjct: 304 EGALNFQLLRCPLNDLSSKVNVVEYNEERYLQEMYPFRDKLLIAGRENGLTQIWVVHDGE 363

Query: 413 --AVDFTDATYSVA-LSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGG 472
              + + +  Y+VA LSE  + ++ +   Y S+ TP +T+  ++++G     +V  V G 
Sbjct: 364 LQQISWDEPLYTVAVLSEQSYDTNEVLIQYESLLTPKTTFGLNLQTGEKQCLQVAPVSGE 423

Query: 473 FDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSR 532
           +D +++  E+ WAT   G KVP++ VY +  +  +G  PL+LYGYGSY    DP F   R
Sbjct: 424 YDRSQFRQEQLWATGRSGVKVPMTAVYLEGALD-NGPAPLILYGYGSYGSNSDPRFDPYR 483

Query: 533 ISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLC 592
           + LL++G ++  A +RGG EMGR WYE+GK+  K+NTFTDFI+ A++LI+  Y S  K+ 
Sbjct: 484 LPLLEKGIVFVTAQVRGGSEMGRGWYEDGKMQNKRNTFTDFIAAAKHLIDQNYTSPTKMA 543

Query: 593 IDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 631
             G SAGGLL+GAV NM  +LFK  V  VPFVDV+TTMLD +IPLTT EW+EWGDPRK+E
Sbjct: 544 ARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTLEWDEWGDPRKQE 603

BLAST of Cp4.1LG01g10000 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 398.3 bits (1022), Expect = 1.7e-109
Identity = 248/695 (35.68%), Postives = 348/695 (50.07%), Query Frame = 0

Query: 53  PVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIY 112
           P A ++ H M L GD RIDNYYWLRDD+R   +++ YLQQEN+Y   VM+  + ++++I 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 113 GEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGA 172
            EI  RI + ++S P  K  Y Y      G EY  Y R+     E      D   T    
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEE-----WDEWET---- 122

Query: 173 PPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGK 232
                +LD N +A +  +Y +G   ++P+N ++A AED      Y +   + ETG    +
Sbjct: 123 -----LLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYPE 182

Query: 233 PLAGVTSYLKWAGN------------------------------DALVYITMD------- 292
            L  V     WA +                              D L+Y   D       
Sbjct: 183 LLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSL 242

Query: 293 ------------------------------------------------------------ 352
                                                                       
Sbjct: 243 HKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNR 302

Query: 353 -------------------EILRPDKDIQ-----LFLNHIVVFEREDGLPKIVVYSLPDI 412
                              E++ P ++I      LF + +VV ER+ GL      SL  I
Sbjct: 303 HGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLT-----SLRQI 362

Query: 413 GEPLRSLEGGRAVDFTDATYSVALS-ESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSIL 472
               R + G   + F D  Y   ++   E  ++ LR+ YSSM TP + ++ DM +G   +
Sbjct: 363 NRKTREVIG---IAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRV 422

Query: 473 KKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEIC 532
            K +T + GF    Y +E  W  A DG +VP+S+VY +   +  G +PLL+YGYGSY   
Sbjct: 423 LK-QTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFR-KGHNPLLVYGYGSYGAS 482

Query: 533 IDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIEN 592
           ID  F  SR+SLLDRGF+Y I H+RGGGE+G+QWYE+GK LKKKNTF D++   + L++ 
Sbjct: 483 IDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKL 542

Query: 593 KYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWE 626
            Y S       G SAGG+L+G  +N RP+LF   +A VPFVDV+TTMLD +IPLTT E+E
Sbjct: 543 GYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFE 602

BLAST of Cp4.1LG01g10000 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.6e-59
Identity = 197/745 (26.44%), Postives = 307/745 (41.21%), Query Frame = 0

Query: 34  HFQSPASSTVVTMSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQE 93
           HF+S     VV    S  PP+ +       L  DV +D Y WLRD  R+N D+ +YL+ E
Sbjct: 26  HFRS-----VVMWDKSLRPPLPRAEPRIRVLHDDVTVDRYGWLRD--RENPDVRAYLEAE 85

Query: 94  NAYTDFVMSGTKQVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFV 153
           N+Y +   +  ++++ ++  EI GR   +  + P + GP+ Y++    G  +  + RR V
Sbjct: 86  NSYAEQATAHLRRLKTELIAEIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPVWWRRPV 145

Query: 154 PPGEEAVSVHDVMPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKG 213
             G                    ++LD N       +Y +G FE S + + +A++ D  G
Sbjct: 146 TGGS-----------------AELVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIG 205

Query: 214 DEIYTVYVIDAETGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDK----------- 273
            E Y + V D   G  V +  AG    + WA ++  ++ T +   RPD+           
Sbjct: 206 AERYELRVRDMSDGRDVWRD-AGSVGQVVWAADNHTLFFTRE---RPDRRQHHQIVRLNV 265

Query: 274 ------------------------------------------------------------ 333
                                                                       
Sbjct: 266 GRGNSEVVFEEANERLAVLVRRSQSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGG 325

Query: 334 ------------------------------------------------------------ 393
                                                                       
Sbjct: 326 QWRRIVMRELGHQIYAEHWYDRFLFRVDDAGPYWRLVSAPIDDPSPSRWEEVVPHRAGVT 385

Query: 394 --DIQLFLNHIVVFEREDGLPKIV---------VYSLPDIGEPLRSLEGGRAVDFTDATY 453
             +I +   H+V+ ERE   P+++            +PD  EP  ++  G +       Y
Sbjct: 386 IDEIHVLEQHLVLLEREGLRPRLISRNRSGRVGAVIVPD--EPSCTIRVGLS---AGGCY 445

Query: 454 SVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGFDTNKYVTERKW 513
           S A     F SS L +  SS  TP +  ++D  +  S++   E  + G+D  +Y+     
Sbjct: 446 SAA--RHPFRSSKLTYSVSSFVTPDTFIEHDFANDRSVV-LCEARVPGYDATQYLATVVM 505

Query: 514 ATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKG------SRISLLDR 573
           A A DG +VP+S+V R+D        P+LL  YG Y I   PSF        +R+SLLDR
Sbjct: 506 AEAEDGVQVPISLVARRDRT---SPGPVLLSVYGCYGIPRLPSFLAWPSSMTARLSLLDR 565

Query: 574 GFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSA 629
              + I H+RGGGE+GR W++     +K+ T TD IS  E LIE  + +R+ + I+G+S 
Sbjct: 566 EVAFGIVHVRGGGELGRPWHDAATRDQKRITHTDLISATEGLIERGFATRDGVVIEGKSG 625

BLAST of Cp4.1LG01g10000 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 4.4e-54
Identity = 136/378 (35.98%), Postives = 202/378 (53.44%), Query Frame = 0

Query: 262 KDIQLFLNHIVVFEREDGLPKIVVYSLPD-IGEPLRSLEGGRAVDF-TDATYSVALSESE 321
           ++I +   H++V ERE   P++V +     +G  +  +E    V     A  S + +   
Sbjct: 328 EEIHVLEEHVIVLEREGIQPRLVAHHRNGRVGPSIVPVEHSCTVTVGLSAGGSYSCARHP 387

Query: 322 FSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGFDTNKYVTERKWATALDGTK 381
           +  S L +   S  TP     +D+ +  S +    T++ GF+   Y      A A DG +
Sbjct: 388 YRVSALTYKICSFVTPDIFIQHDLLTDKSKV-LYRTLVSGFEPELYEARVVMAKAEDGVE 447

Query: 382 VPLSIVYRKDLVKLDGSD-PLLLYGYGSYEICIDPSFKG------SRISLLDRGFIYTIA 441
           VP+SIV R+D     G D P+LL  YG Y     P+F G      +R+SLLDRG  + I 
Sbjct: 448 VPISIVARRD----RGEDGPVLLNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIV 507

Query: 442 HIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLLIGA 501
           H+RGGGE+GR W+E     +K+ T TD I+ AE L+E+++ SR+ + I+GRSAGG  + A
Sbjct: 508 HVRGGGELGRAWHEAATRDQKRLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLA 567

Query: 502 VLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDN 561
              +RPDLF+A +A VP  D++ T LD T+P    E  E+GDP     Y Y++SY P  N
Sbjct: 568 AAVLRPDLFRAVLAEVPLADIIDTELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYN 627

Query: 562 IKA-RHYPDILVTTGLNDPRVLYSEPAKFVAKLRDMKTD-DNLLLFKCELGAGHFSKSGR 621
           +   R YP   +   L+D +VLY +PA++VA+ R    D D  L+F+  +  GH   S  
Sbjct: 628 LTPDRRYPPTYIDAALHDSQVLYYQPARYVAQRRSKAVDRDPDLIFRTRMIGGHMGVSHG 687

Query: 622 FEKLQEDAFTYAFILKSL 629
               +E AF  A+IL  L
Sbjct: 688 PGVAEEAAFRMAWILHRL 700

BLAST of Cp4.1LG01g10000 vs. NCBI nr
Match: XP_023516940.1 (uncharacterized protein LOC111780697 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1242 bits (3213), Expect = 0.0
Identity = 636/756 (84.13%), Postives = 636/756 (84.13%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEH 60
           MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEH
Sbjct: 1   MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120

Query: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILD 180
           EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILD
Sbjct: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILD 180

Query: 181 ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240
           ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY
Sbjct: 181 ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240

Query: 241 LKWAGNDALVYITMDEILRPDK-------------------------------------- 300
           LKWAGNDALVYITMDEILRPDK                                      
Sbjct: 241 LKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASESKKYLF 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 IASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVVA 360

Query: 361 ----------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420
                                 DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG
Sbjct: 361 CPLDNISATMVLLPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420

Query: 421 GRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGF 480
           GRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGF
Sbjct: 421 GRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGF 480

Query: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540
           DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI
Sbjct: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540

Query: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCI 600
           SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCI
Sbjct: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCI 600

Query: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 636
           DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF
Sbjct: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 660

BLAST of Cp4.1LG01g10000 vs. NCBI nr
Match: KAG7031880.1 (Protease 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1217 bits (3150), Expect = 0.0
Identity = 624/756 (82.54%), Postives = 630/756 (83.33%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEH 60
           MKPLQQNVIFGLVRRS ILFIPFAGSPSVASFRHFQS ASSTVVTMS+SHSPPVAKKVEH
Sbjct: 1   MKPLQQNVIFGLVRRSFILFIPFAGSPSVASFRHFQSLASSTVVTMSHSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRKNAD+ISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNADVISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120

Query: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILD 180
           EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVP GEEAVSVHDVMPTGPGAPPEHVILD
Sbjct: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPRGEEAVSVHDVMPTGPGAPPEHVILD 180

Query: 181 ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240
           ENDKAQNQSY+CIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY
Sbjct: 181 ENDKAQNQSYFCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240

Query: 241 LKWAGNDALVYITMDEILRPDK-------------------------------------- 300
           LKWAGNDALVYITMDEILRPDK                                      
Sbjct: 241 LKWAGNDALVYITMDEILRPDKAWLHKLGPEQSTDTCLYHEKDDMFSLDLQASESKKYLF 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 IASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVVA 360

Query: 361 ----------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420
                                 DIQL LNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG
Sbjct: 361 CPLNNISATTILLPHRESVKIQDIQLSLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420

Query: 421 GRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGF 480
           GRAVDFTDATYSV LSESEFSSSILRFCYSSMRTPPSTYDYDM SGVSILKKVETVLGGF
Sbjct: 421 GRAVDFTDATYSVDLSESEFSSSILRFCYSSMRTPPSTYDYDMTSGVSILKKVETVLGGF 480

Query: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540
           DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI
Sbjct: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540

Query: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCI 600
           SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCS+EKLCI
Sbjct: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSKEKLCI 600

Query: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 636
           DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF
Sbjct: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 660

BLAST of Cp4.1LG01g10000 vs. NCBI nr
Match: KAG6601075.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1216 bits (3146), Expect = 0.0
Identity = 624/756 (82.54%), Postives = 629/756 (83.20%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFAGSPSVASFRHFQSPASSTVVTMSYSHSPPVAKKVEH 60
           MK LQQNVIFGLVRRS ILFIPFAGSPSVASFRHFQS ASSTVVTMS+SHSPPVAKKVEH
Sbjct: 1   MKALQQNVIFGLVRRSFILFIPFAGSPSVASFRHFQSLASSTVVTMSHSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRKNAD+ISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNADVISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIK 120

Query: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEHVILD 180
           EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVP GEEAVSVHDVMPTGPGAPPEHVILD
Sbjct: 121 EDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPRGEEAVSVHDVMPTGPGAPPEHVILD 180

Query: 181 ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240
           ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY
Sbjct: 181 ENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSY 240

Query: 241 LKWAGNDALVYITMDEILRPDK-------------------------------------- 300
           LKWAGNDALVYITMDEILRPDK                                      
Sbjct: 241 LKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASESKKYLF 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 IASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVVA 360

Query: 361 ----------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420
                                 DIQL LNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG
Sbjct: 361 CPLDNISATTILLPHRESVKIQDIQLSLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEG 420

Query: 421 GRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETVLGGF 480
           GRAVDFTDATYSV LSESEFSSSILRFCYSSMRTPPSTYDYDM SGVSILKKVETVLGGF
Sbjct: 421 GRAVDFTDATYSVDLSESEFSSSILRFCYSSMRTPPSTYDYDMTSGVSILKKVETVLGGF 480

Query: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540
           DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI
Sbjct: 481 DTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRI 540

Query: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCI 600
           SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCS+EKLCI
Sbjct: 541 SLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSKEKLCI 600

Query: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 636
           DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF
Sbjct: 601 DGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEF 660

BLAST of Cp4.1LG01g10000 vs. NCBI nr
Match: XP_022957416.1 (uncharacterized protein LOC111458824 [Cucurbita moschata])

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 581/711 (81.72%), Postives = 586/711 (82.42%), Query Frame = 0

Query: 46  MSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTK 105
           MS+SHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNAD+ISYLQQENAYTDFVMSGTK
Sbjct: 1   MSHSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADVISYLQQENAYTDFVMSGTK 60

Query: 106 QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDV 165
           QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVP GEEAVSVHDV
Sbjct: 61  QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPRGEEAVSVHDV 120

Query: 166 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE 225
           MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIY VYVIDAE
Sbjct: 121 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYKVYVIDAE 180

Query: 226 TGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDK----------------------- 285
           TGAPVGKPL GVTSYLKWAGNDALVYITMDEILRPDK                       
Sbjct: 181 TGAPVGKPLTGVTSYLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDM 240

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 241 FSLDLQASESKKYLFIASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFF 300

Query: 346 -------------------------------------DIQLFLNHIVVFEREDGLPKIVV 405
                                                DIQLFLNHIVVFEREDGLPKIVV
Sbjct: 301 IRRRSEEIFNSEVVACPLDNISATTVLLPHRESVKIQDIQLFLNHIVVFEREDGLPKIVV 360

Query: 406 YSLPDIGEPLRSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKS 465
           YSLPDIGEPLRSLEGGRAVDFTDATYSV LSESEFSSSILRFCYSSMRTPPSTYDYDM S
Sbjct: 361 YSLPDIGEPLRSLEGGRAVDFTDATYSVDLSESEFSSSILRFCYSSMRTPPSTYDYDMTS 420

Query: 466 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 525
           GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG
Sbjct: 421 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 480

Query: 526 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 585
           SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE
Sbjct: 481 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 540

Query: 586 YLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 636
           YLIENKYCS+EKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT
Sbjct: 541 YLIENKYCSKEKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 600

BLAST of Cp4.1LG01g10000 vs. NCBI nr
Match: XP_038893092.1 (protease 2 [Benincasa hispida])

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 585/761 (76.87%), Postives = 606/761 (79.63%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFA----GSPSVASFRHFQSPASSTVVTMSYSHSPPVAK 60
           MKPLQQN IFGLVRRS IL IP        PSVASFRHF+SP    V TMS SHSPPVA 
Sbjct: 1   MKPLQQNSIFGLVRRSLILLIPVVYLSPALPSVASFRHFRSP----VATMSQSHSPPVAN 60

Query: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIR 120
           KVEHKMELFGDVRIDNYYWLRDDSRKN+D+ISYLQQENAYTDFVMSGTKQVEEQIY EIR
Sbjct: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDFVMSGTKQVEEQIYSEIR 120

Query: 121 GRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEE-AVSVHDVMPTGPGAPPE 180
           GRIKEDDISVPERKGPYYYY+RTL+GKEYVQYCRRFVP GEE A+SVHD MPTGP APPE
Sbjct: 121 GRIKEDDISVPERKGPYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPDAPPE 180

Query: 181 HVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLA 240
           HVILDEN KAQNQSYY IG FEVSPNNKLVAYAEDTKGDEIYTVY+IDAETGAPVGKPLA
Sbjct: 181 HVILDENVKAQNQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLA 240

Query: 241 GVTSYLKWAGNDALVYITMDEILRPDK--------------------------------- 300
           GVTSYLKWAGNDALVYITMDEILRPDK                                 
Sbjct: 241 GVTSYLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASES 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KKYLFIASESKFTRFNFYLDVSKPEDGLVVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFN 360

Query: 361 ---------------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPL 420
                                      DIQLFLN I++FEREDGLPKIVVYSLPDIGEPL
Sbjct: 361 SEVVACPLDNTSATTVILPHRESVKIQDIQLFLNQIIIFEREDGLPKIVVYSLPDIGEPL 420

Query: 421 RSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVET 480
           RSLEGGRAVDFTDATYSV  SESEFSSS+LRFCYSSM+TPPSTYDYDMK+GVSILKKVET
Sbjct: 421 RSLEGGRAVDFTDATYSVDASESEFSSSVLRFCYSSMKTPPSTYDYDMKTGVSILKKVET 480

Query: 481 VLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSF 540
           VLGGFDTNKYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE+C+DPSF
Sbjct: 481 VLGGFDTNKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEVCVDPSF 540

Query: 541 KGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSR 600
           K SRISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKY S+
Sbjct: 541 KASRISLLDRGFIYAIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYGSK 600

Query: 601 EKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP 636
           EKLCI+GRSAGGLLIG+VLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP
Sbjct: 601 EKLCINGRSAGGLLIGSVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP 660

BLAST of Cp4.1LG01g10000 vs. ExPASy TrEMBL
Match: A0A6J1GZ53 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458824 PE=3 SV=1)

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 581/711 (81.72%), Postives = 586/711 (82.42%), Query Frame = 0

Query: 46  MSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTK 105
           MS+SHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNAD+ISYLQQENAYTDFVMSGTK
Sbjct: 1   MSHSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADVISYLQQENAYTDFVMSGTK 60

Query: 106 QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDV 165
           QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVP GEEAVSVHDV
Sbjct: 61  QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPRGEEAVSVHDV 120

Query: 166 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE 225
           MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIY VYVIDAE
Sbjct: 121 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYKVYVIDAE 180

Query: 226 TGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDK----------------------- 285
           TGAPVGKPL GVTSYLKWAGNDALVYITMDEILRPDK                       
Sbjct: 181 TGAPVGKPLTGVTSYLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDM 240

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 241 FSLDLQASESKKYLFIASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFF 300

Query: 346 -------------------------------------DIQLFLNHIVVFEREDGLPKIVV 405
                                                DIQLFLNHIVVFEREDGLPKIVV
Sbjct: 301 IRRRSEEIFNSEVVACPLDNISATTVLLPHRESVKIQDIQLFLNHIVVFEREDGLPKIVV 360

Query: 406 YSLPDIGEPLRSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKS 465
           YSLPDIGEPLRSLEGGRAVDFTDATYSV LSESEFSSSILRFCYSSMRTPPSTYDYDM S
Sbjct: 361 YSLPDIGEPLRSLEGGRAVDFTDATYSVDLSESEFSSSILRFCYSSMRTPPSTYDYDMTS 420

Query: 466 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 525
           GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG
Sbjct: 421 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 480

Query: 526 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 585
           SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE
Sbjct: 481 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 540

Query: 586 YLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 636
           YLIENKYCS+EKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT
Sbjct: 541 YLIENKYCSKEKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 600

BLAST of Cp4.1LG01g10000 vs. ExPASy TrEMBL
Match: A0A6J1KIC7 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111493518 PE=3 SV=1)

HSP 1 Score: 1130 bits (2924), Expect = 0.0
Identity = 578/711 (81.29%), Postives = 584/711 (82.14%), Query Frame = 0

Query: 46  MSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTK 105
           MSYS SPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNAD+ISYLQQENAYTDFVMSGTK
Sbjct: 1   MSYSQSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADVISYLQQENAYTDFVMSGTK 60

Query: 106 QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDV 165
           QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVP GEE VSVHDV
Sbjct: 61  QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPRGEEVVSVHDV 120

Query: 166 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE 225
           MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE
Sbjct: 121 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE 180

Query: 226 TGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDK----------------------- 285
           TGAPVGKPLA VTSYLKWAGNDALVYITMDEILRPDK                       
Sbjct: 181 TGAPVGKPLASVTSYLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDM 240

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 241 FSLDLQASESKKYLFIASESKFTRFNFYLDVSRPEDGLTVLTPRVDGVDTFPSHRGNHFF 300

Query: 346 -------------------------------------DIQLFLNHIVVFEREDGLPKIVV 405
                                                DIQLFLNHIVVFEREDGLPKIVV
Sbjct: 301 IRRRSEEIFNSEVVACPLDNISATTVLLPHRESVKIQDIQLFLNHIVVFEREDGLPKIVV 360

Query: 406 YSLPDIGEPLRSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKS 465
           YSLPDIGEPLRSLEGGRAVDFTDATYSV LSESEFSSSILRFCYSS+RTPPSTYDYDM S
Sbjct: 361 YSLPDIGEPLRSLEGGRAVDFTDATYSVDLSESEFSSSILRFCYSSLRTPPSTYDYDMTS 420

Query: 466 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 525
           GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG
Sbjct: 421 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 480

Query: 526 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 585
           SYEICIDPSFKGSRISLLDRGFIYTIA+IRGGGEMGRQWYENGKLLKKKNTFTDFISCAE
Sbjct: 481 SYEICIDPSFKGSRISLLDRGFIYTIANIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 540

Query: 586 YLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 636
           YLIENKYCS+EKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT
Sbjct: 541 YLIENKYCSKEKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 600

BLAST of Cp4.1LG01g10000 vs. ExPASy TrEMBL
Match: A0A6J1G021 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1)

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 577/760 (75.92%), Postives = 601/760 (79.08%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFA----GSPSVASFRHFQSPASSTVVTMSYSHSPPVAK 60
           MKPLQQN IFGLVRRS +LF+P        PSVAS RHF+S A+    TMS SHSPPVAK
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAA----TMSNSHSPPVAK 60

Query: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIR 120
           KVEHKMELFGDVRIDNYYWLRDDSRKN+D+ISYLQQENAYT+ VMSGTKQVEEQIY EIR
Sbjct: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIR 120

Query: 121 GRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEH 180
           GRIKEDDISVPERKG YYYY+RTL GKEYVQYCRRFVP GEE++SVHD MPTGPGAPPEH
Sbjct: 121 GRIKEDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRGEESLSVHDTMPTGPGAPPEH 180

Query: 181 VILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAG 240
           VILDEN KAQNQSYY IGAFEVSPNNKLVAYAEDTKGDEIYTVY+IDAETGA VGKPL G
Sbjct: 181 VILDENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVG 240

Query: 241 VTSYLKWAGNDALVYITMDEILRPDK---------------------------------- 300
           VTSYL WAG+DALVYITMDEILRPDK                                  
Sbjct: 241 VTSYLNWAGDDALVYITMDEILRPDKAWLHKLETEQSTDTCLYHEKDDMFSLDLQATESK 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KYLFIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNS 360

Query: 361 --------------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLR 420
                                     DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPL+
Sbjct: 361 EVVACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLK 420

Query: 421 SLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETV 480
           SLEGGRAVDFTDATYSV LS+SEFSSSILRFCYSSMRTPPSTYDYDMK+GVSILKKVE V
Sbjct: 421 SLEGGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAV 480

Query: 481 LGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFK 540
           LGGFD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK
Sbjct: 481 LGGFDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFK 540

Query: 541 GSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSRE 600
           GSRISLLDRGFIY IAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+ AEYLIENKYCS+E
Sbjct: 541 GSRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKE 600

Query: 601 KLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR 636
           KLCI+GRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR
Sbjct: 601 KLCINGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR 660

BLAST of Cp4.1LG01g10000 vs. ExPASy TrEMBL
Match: A0A6J1HU74 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1)

HSP 1 Score: 1118 bits (2893), Expect = 0.0
Identity = 577/760 (75.92%), Postives = 599/760 (78.82%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFA----GSPSVASFRHFQSPASSTVVTMSYSHSPPVAK 60
           MKPLQQN IFGLVRRS +LF+P        P VAS RHF+S A+    TMS SHSPPVAK
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPLVASLRHFRSAAA----TMSNSHSPPVAK 60

Query: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIR 120
           KVEHKMELFGDVRIDNYYWLRDDSRKN+D+ISYLQQENAYTD VMSGTKQVEEQIY EIR
Sbjct: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIR 120

Query: 121 GRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTGPGAPPEH 180
           GRIKEDDISVPERKG YYYY+RTL GKEYVQYCRRFVP GEE++SVHD MPTGPGAPPEH
Sbjct: 121 GRIKEDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRGEESLSVHDTMPTGPGAPPEH 180

Query: 181 VILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAG 240
           VILDEN KAQNQSYY IGAFEVSPNNKLVAYAEDTKGDEIYTVY+IDAETGA VGKPL G
Sbjct: 181 VILDENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVG 240

Query: 241 VTSYLKWAGNDALVYITMDEILRPDK---------------------------------- 300
           VTSYL WAG+DALVYITMDEILRPDK                                  
Sbjct: 241 VTSYLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESK 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KYLFIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFLIRRRSAEIFNS 360

Query: 361 --------------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLR 420
                                     DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLR
Sbjct: 361 EVVACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLR 420

Query: 421 SLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVETV 480
           SLEGGRAVDFTDATYSV LS+SEFSS+ILRFCYSSMRTPPSTYDYDMK+GVSILKKVE V
Sbjct: 421 SLEGGRAVDFTDATYSVYLSDSEFSSTILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAV 480

Query: 481 LGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFK 540
           LGGFD   YVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK
Sbjct: 481 LGGFDITNYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFK 540

Query: 541 GSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSRE 600
           GSRISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFI+ AEYLIENKYCS+E
Sbjct: 541 GSRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFIASAEYLIENKYCSKE 600

Query: 601 KLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR 636
           KLCI+GRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR
Sbjct: 601 KLCINGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPR 660

BLAST of Cp4.1LG01g10000 vs. ExPASy TrEMBL
Match: A0A5A7STX7 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003340 PE=3 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 566/761 (74.38%), Postives = 599/761 (78.71%), Query Frame = 0

Query: 1   MKPLQQNVIFGLVRRSAILFIPFA----GSPSVASFRHFQSPASSTVVTMSYSHSPPVAK 60
           M  LQQN IFG++RRS ILFIP        PSVASFRHF+SP    V TMS SHSPPVA 
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSP----VATMSQSHSPPVAN 60

Query: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIR 120
           KVEHKMELFGDVRIDNYYWLRDDSRKN D++SYLQQENAYTD VMSGTK+VE+QIY EIR
Sbjct: 61  KVEHKMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIR 120

Query: 121 GRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEE-AVSVHDVMPTGPGAPPE 180
           GRIKEDD++VPER+G YYYY+RTL+GKEYVQYCRRFVP GEE A+SVHD MPTGP APPE
Sbjct: 121 GRIKEDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPE 180

Query: 181 HVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLA 240
           HVILDEN KA+NQSYY IG FEVSPNNKLVAYAEDTKGDEIYTVY+IDAETGAPVGKPL 
Sbjct: 181 HVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLV 240

Query: 241 GVTSYLKWAGNDALVYITMDEILRPDK--------------------------------- 300
           GVTSYLKWAG+DALVYITMDEILRPDK                                 
Sbjct: 241 GVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASES 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFN 360

Query: 361 ---------------------------DIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPL 420
                                      DIQLFLNHIV+FEREDGLPK+VVYSLPDIGEPL
Sbjct: 361 SEVVACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPL 420

Query: 421 RSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGVSILKKVET 480
           ++LEGGRAVDF DATYSV   ESEFSSSILRFCYSSM+TP STYDYDMK+GVS+LKKVET
Sbjct: 421 KTLEGGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVET 480

Query: 481 VLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSF 540
           VLGGFD+NKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEIC+DPSF
Sbjct: 481 VLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSF 540

Query: 541 KGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSR 600
           K SRISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFIS AEYLIENKYCS+
Sbjct: 541 KPSRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSK 600

Query: 601 EKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP 636
           EKLCI+GRSAGGLLIGAV+NMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP
Sbjct: 601 EKLCINGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDP 660

BLAST of Cp4.1LG01g10000 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 898.7 bits (2321), Expect = 2.8e-261
Identity = 444/710 (62.54%), Postives = 512/710 (72.11%), Query Frame = 0

Query: 46  MSYSHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTK 105
           M+ S SPPVAKKVEH ME+FGDVR+DNYYWLRDDSR N D++SYL++EN YTDFVMSGTK
Sbjct: 1   MAESRSPPVAKKVEHVMEMFGDVRVDNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTK 60

Query: 106 QVEEQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDV 165
           Q E Q++ EIRGRIKEDDIS P RKGPYYYY++ L GKEY+Q+CRR +   +   SV+D 
Sbjct: 61  QFENQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEPSVYDT 120

Query: 166 MPTGPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAE 225
           MPTGP APPEHVILDEN KAQ   YY IGAF+ SP++KLVAYAEDTKGDEIYTV VID+E
Sbjct: 121 MPTGPDAPPEHVILDENTKAQEHDYYRIGAFKASPDHKLVAYAEDTKGDEIYTVNVIDSE 180

Query: 226 TGAPVGKPLAGVTSYLKWAGNDALVYITMDEILRPDK----------------------- 285
              PVG+ L G+TSYL+WAGNDAL+YITMDEILRPDK                       
Sbjct: 181 ALKPVGQQLKGLTSYLEWAGNDALLYITMDEILRPDKVWLHKLGTEQSSDVCLYHEKDDM 240

Query: 286 ------------------------------------------------------------ 345
                                                                       
Sbjct: 241 FSLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRVDGIDSSVSHRGNHFF 300

Query: 346 -------------------------------------DIQLFLNHIVVFEREDGLPKIVV 405
                                                +IQLF +H+ VFERE+GL KI V
Sbjct: 301 IQRRSTEFYNSELIACPVDDTSKTTVLLPHRESVKIQEIQLFRDHLAVFERENGLQKITV 360

Query: 406 YSLPDIGEPLRSLEGGRAVDFTDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKS 465
           + LP  G+PL  L+GGR V F D  YSV  +ESEFSS +LRF Y SM+TPPS YDYDM S
Sbjct: 361 HRLPAEGQPLEGLQGGRNVSFVDPVYSVDSTESEFSSRVLRFKYCSMKTPPSVYDYDMDS 420

Query: 466 GVSILKKVETVLGGFDTNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYG 525
           G S++KK++TVLGGFD + YVTERKW  A DGT++P+SIVY K L KLDGSDPLLLYGYG
Sbjct: 421 GTSVVKKIDTVLGGFDASNYVTERKWVAASDGTQIPMSIVYNKKLAKLDGSDPLLLYGYG 480

Query: 526 SYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAE 585
           SYEI +DP FK SR+SLLDRGF + IAH+RGGGEMGRQWYENGKLLKKKNTFTDFI+CAE
Sbjct: 481 SYEISVDPYFKASRLSLLDRGFTFVIAHVRGGGEMGRQWYENGKLLKKKNTFTDFIACAE 540

Query: 586 YLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLT 636
            LIE KYCS+EKLC++GRSAGGLL+GAV+NMRPDLFK  +AGVPFVDVLTTMLDPTIPLT
Sbjct: 541 RLIELKYCSKEKLCMEGRSAGGLLMGAVVNMRPDLFKVVIAGVPFVDVLTTMLDPTIPLT 600

BLAST of Cp4.1LG01g10000 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.4e-74
Identity = 206/725 (28.41%), Postives = 324/725 (44.69%), Query Frame = 0

Query: 49  SHSPPVAKKVEHKMELFGDVRIDNYYWLRDDSRKNADIISYLQQENAYTDFVMSGTKQVE 108
           + +PPV KK+   +   G  R D ++W+++    + D + +L++EN+Y+   M+ T+ + 
Sbjct: 37  TEAPPVPKKIPFAISSHGITRQDPFHWMKNTD--DTDFVDFLKRENSYSQAFMADTETLR 96

Query: 109 EQIYGEIRGRIKEDDISVPERKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPT 168
             ++ E++ RI E+  + PER G + Y +    GKEY   CRR      E    + +   
Sbjct: 97  RDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPKGKEYPLLCRRL-----EKGKTNWLSGL 156

Query: 169 GPGAPPEHVILDENDKAQNQSYYCIGAFEVSPNNKLVAYAEDTKGDEIYTVYVIDAETGA 228
             G   E V+LD N  A+   Y  +G   VSP++  +AY  D +GD I   Y +  E   
Sbjct: 157 FRG-EEEEVVLDWNQIAEQFGYVHVGVCRVSPDHNYLAYTVDPEGDGITLFYTVTDENQR 216

Query: 229 P-------------------------------------------------------VGKP 288
           P                                                         KP
Sbjct: 217 PHRVVVTNVESDGRDDAVVFTERDSSFCVDITTTKDGKFVTINSNSRTSSEVYIVNADKP 276

Query: 289 LAGV-----------------------------TSYLKWAGNDALVYIT----------- 348
           +AG+                              +  +W+G     Y+T           
Sbjct: 277 MAGLQRTRERVPGVQCFLEHHNGFFYILTNSPSNAISEWSGEG--YYLTRCLVEEIEASD 336

Query: 349 MDEILRPD-----KDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLEGGRAVDF-- 408
              + RPD     +D+ +F +++V++  + GLP +    +P I    + ++      F  
Sbjct: 337 WQTVFRPDDDVVIQDMDMFNDYLVLYLNKKGLPMLCSIDMP-IKANTKHMDDLVPWYFPL 396

Query: 409 -TDATYSVALSESEFSSSILRFCYSSMRTPPSTYDYDMKSGV-SILKKVETVLGGFDTNK 468
             D+      S  +F SSI R   SS   P +  DYD+   + SI+++   V+   D++K
Sbjct: 397 PVDSCSVAPGSNHDFQSSIYRVVLSSPVIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSK 456

Query: 469 ----------------------------------------YVTERKWATALDGTKVPLSI 528
                                                   YV ER+  ++ DG +VPL+I
Sbjct: 457 PWYTADRSTENNGQLNDRTSEGEDGQLDSRMPKWEDLSDTYVCERQEVSSHDGVEVPLTI 516

Query: 529 VYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRISLLDRGFIYTIAHIRGGGEMGRQW 588
           +Y ++  K   S P +L GYG+Y   +D S+  +R+S+LDRG++   A +RGGG     W
Sbjct: 517 LYSREAWKKSES-PGMLIGYGAYGEVLDKSWCTNRLSMLDRGWVIAFADVRGGGSGEFSW 576

Query: 589 YENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAA 627
           +++G    K+N+  DFI  A+YL+E  Y  R  L   G SAG +L  A +NM P LF+A 
Sbjct: 577 HKSGTRSLKQNSIQDFIYSAKYLVEKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAV 636

BLAST of Cp4.1LG01g10000 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 221.1 bits (562), Expect = 2.6e-57
Identity = 196/740 (26.49%), Postives = 308/740 (41.62%), Query Frame = 0

Query: 71  DNYYWLR--DDSRKNADIISYLQQENAYTDFVMSGTKQVEEQIYGEIRGRIKEDDISVPE 130
           D Y W+   +D      +  Y++QE  YT+ V++ T +++ ++  E+  R+  +  + P 
Sbjct: 58  DPYSWMSKLEDKVAMRHMDIYMEQEEKYTEAVLADTDRIQTKLQSEMASRLSFELSTPPL 117

Query: 131 RKGPYYYYKRTLDGKEYVQYCRRFVPPGEEAVSVHDVMPTG----PGAPPEHVILDENDK 190
           R GP+ YY+R  +GK+Y   CRR     EE +S H     G     G   E  +LD N +
Sbjct: 118 RWGPWLYYRRVEEGKQYPVLCRRLASLHEEFIS-HKSPAAGFDYTSGKRIEQKLLDYNQE 177

Query: 191 AQNQSYYCIGAF-EVSPNNKLVAYAEDTKGDEIYTVYVIDAETGAPVGKPLAGVTSYLKW 250
           A+    Y      E+SP++K +AY    K ++ + + V +  +GA   KP A   S + W
Sbjct: 178 AERFGGYAYEEMSEISPDHKFLAYTMYDKDNDYFKLCVRNLNSGALCSKPHADRVSNIAW 237

Query: 251 AGN-DALVYITMDEILRP-----------DKDIQLFLN-----HIVVFERED-------- 310
           A N  AL+Y+  D+  RP           D+D+ L        H+ +   +D        
Sbjct: 238 AKNGQALLYVVTDQKKRPCRIYCSTIGSTDEDVLLHEEFEGNVHVNIRHTKDFHFVTVNT 297

Query: 311 ------------------GLPKIVVYSLPD------------------------------ 370
                             GL  +  ++ P                               
Sbjct: 298 FSTTFSKVFLINAADPFSGLALVWEHNAPAHCIIEHHQGFLYLFTNASNDGGTVDHHYLL 357

Query: 371 -----------------IGEPLRSLEGGRAVDFTDATYSVALSES--------------- 430
                            I +P   +E    VDF     S+ + E                
Sbjct: 358 RSPVHFSSCQRIWETVFINDPELIIED---VDFCKKHLSLIVKEMQSFKICVVDLPLKTK 417

Query: 431 -----------------------------EFSSSILRFCYSSMRTPPSTYDYDMKSG--- 490
                                        +F+S  +RF  SS+  P +  DYD+ +G   
Sbjct: 418 RVPVHLRDIKPRYLPLPKHVSQIFPGTNYDFNSPTMRFTISSLVMPDAVVDYDLLNGKWN 477

Query: 491 ----VSILKKVETVLGG------------------FDTNK---------------YVTER 550
                ++L +   VL G                  FDT                 Y  + 
Sbjct: 478 IVQQQNMLHERTRVLYGTANSTESPNIPSGTRTVSFDTEDTTAENDNLWNDLTEFYACDY 537

Query: 551 KWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRISLLDRGFIY 610
              ++ DG  VPLSIVY +   K +   P LL+ +G+Y   +D  ++    SLLDRG++ 
Sbjct: 538 HEVSSHDGAMVPLSIVYSR-AQKEENQKPGLLHVHGAYGEMLDKRWRSELKSLLDRGWVL 597

Query: 611 TIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLL 629
             A +RGGG  G++W+++G+  KK N+  D+I CA+YL+EN      KL   G SAGGL+
Sbjct: 598 AYADVRGGGGKGKKWHQDGRGAKKLNSIKDYIQCAKYLVENNIVEENKLAGWGYSAGGLV 657

BLAST of Cp4.1LG01g10000 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 193.0 bits (489), Expect = 7.5e-49
Identity = 118/321 (36.76%), Postives = 171/321 (53.27%), Query Frame = 0

Query: 327 FCYSSMRTPPSTYDYDMKSGVSILKKV-ETVLGGFDTNKYVTERKWATALDGTKVPLSIV 386
           F ++S  TP   Y  D+ +    +K   E  + GFD   +   + +  + DGTK+P+ IV
Sbjct: 474 FSFTSFLTPGVIYKCDLANESPEVKVFREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIV 533

Query: 387 YRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRISLLDR-GFIYTIAHIRGGGEMGRQW 446
            +KD +KLDGS P LLY YG + I I PSF  SRI L    G ++  A+IRGGGE G +W
Sbjct: 534 AKKD-IKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEW 593

Query: 447 YENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAA 506
           ++ G L KK+N F DFIS AEYL+   Y    KLCI+G S GGLL+GA +N RPDL+  A
Sbjct: 594 HKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYGCA 653

Query: 507 VAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNIKAR-------- 566
           +A V  +D+L           TS   ++G    EE + ++  YSP+ N+K          
Sbjct: 654 LAHVGVMDMLRFHKFTIGHAWTS---DYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHL 713

Query: 567 -HYPDILVTTGLNDPRVLYSEPAKFVAKLR-------DMKTDDNLLLFKCELGAGHFSKS 626
             YP  ++ T  +D RV+     K +A L+       D     N ++ + E+ AGH +  
Sbjct: 714 VQYPSTMLLTADHDDRVVPLHSLKLLATLQHVLCTSLDNSPQMNPIIGRIEVKAGHGAGR 773

Query: 627 GRFEKLQEDAFTYAFILKSLN 630
              + + E A  Y+F+ K +N
Sbjct: 774 PTQKMIDEAADRYSFMAKMVN 790

BLAST of Cp4.1LG01g10000 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 192.6 bits (488), Expect = 9.8e-49
Identity = 117/318 (36.79%), Postives = 171/318 (53.77%), Query Frame = 0

Query: 327 FCYSSMRTPPSTYDYDMKSGVSILKKV-ETVLGGFDTNKYVTERKWATALDGTKVPLSIV 386
           F ++S  TP   Y  D+ +    +K   E  + GFD   +   + +  + DGTK+P+ IV
Sbjct: 474 FSFTSFLTPGVIYKCDLANESPEVKVFREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIV 533

Query: 387 YRKDLVKLDGSDPLLLYGYGSYEICIDPSFKGSRISLLDR-GFIYTIAHIRGGGEMGRQW 446
            +KD +KLDGS P LLY YG + I I PSF  SRI L    G ++  A+IRGGGE G +W
Sbjct: 534 AKKD-IKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEW 593

Query: 447 YENGKLLKKKNTFTDFISCAEYLIENKYCSREKLCIDGRSAGGLLIGAVLNMRPDLFKAA 506
           ++ G L KK+N F DFIS AEYL+   Y    KLCI+G S GGLL+GA +N RPDL+  A
Sbjct: 594 HKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYGCA 653

Query: 507 VAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNIKAR-------- 566
           +A V  +D+L           TS   ++G    EE + ++  YSP+ N+K          
Sbjct: 654 LAHVGVMDMLRFHKFTIGHAWTS---DYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHL 713

Query: 567 -HYPDILVTTGLNDPRVLYSEPAKFVAKLRDMKTDD----NLLLFKCELGAGHFSKSGRF 626
             YP  ++ T  +D RV+     K +A +     D+    N ++ + E+ AGH +     
Sbjct: 714 VQYPSTMLLTADHDDRVVPLHSLKLLAHVLCTSLDNSPQMNPIIGRIEVKAGHGAGRPTQ 773

Query: 627 EKLQEDAFTYAFILKSLN 630
           + + E A  Y+F+ K +N
Sbjct: 774 KMIDEAADRYSFMAKMVN 787

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O078342.1e-12537.66Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
Q595361.9e-11836.18Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
P245551.7e-10935.68Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
P556271.6e-5926.44Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
P556564.4e-5435.98Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
XP_023516940.10.084.13uncharacterized protein LOC111780697 [Cucurbita pepo subsp. pepo][more]
KAG7031880.10.082.54Protease 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6601075.10.082.54Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022957416.10.081.72uncharacterized protein LOC111458824 [Cucurbita moschata][more]
XP_038893092.10.076.87protease 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GZ530.081.72Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458824 PE=3 SV=1[more]
A0A6J1KIC70.081.29Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111493518 PE=3 SV=1[more]
A0A6J1G0210.075.92Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1[more]
A0A6J1HU740.075.92Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1[more]
A0A5A7STX70.074.38Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
Match NameE-valueIdentityDescription
AT1G50380.12.8e-26162.54Prolyl oligopeptidase family protein [more]
AT1G69020.11.4e-7428.41Prolyl oligopeptidase family protein [more]
AT5G66960.12.6e-5726.49Prolyl oligopeptidase family protein [more]
AT1G76140.17.5e-4936.76Prolyl oligopeptidase family protein [more]
AT1G76140.29.8e-4936.79Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 540..555
score: 47.37
coord: 452..471
score: 48.42
coord: 482..502
score: 64.41
coord: 398..416
score: 50.42
coord: 558..580
score: 47.83
coord: 424..448
score: 55.58
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 413..629
e-value: 3.6E-67
score: 226.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 336..633
e-value: 3.5E-106
score: 357.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 365..610
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 52..261
e-value: 1.1E-54
score: 185.8
coord: 263..351
e-value: 1.1E-9
score: 37.7
NoneNo IPR availableGENE3D2.130.10.120coord: 122..262
e-value: 4.3E-39
score: 136.3
NoneNo IPR availablePANTHERPTHR11757:SF17B, PUTATIVE-RELATEDcoord: 47..262
coord: 263..633
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 263..633
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 47..262
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 269..361
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 48..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g10000.1Cp4.1LG01g10000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity