Cp4.1LG01g08870 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g08870
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCytochrome C1 family
LocationCp4.1LG01: 4578705 .. 4584031 (-)
RNA-Seq ExpressionCp4.1LG01g08870
SyntenyCp4.1LG01g08870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCATACATATAGATGAAAAAATATGGTAAAATTTACTGATTTGTTAGAATAAAATTAAATTTATATCTAATAATTAATTGATTTAAAAATNTTTATCAAACAAATTTTGAGATAAAAATTAATTCTTTTTTCACACATATAGATTAAATTTATATCTAATAATTAATTGATTTAAAATTTTAGCTCTTGAAAATTGCCGGTTTTTGCAGAATTGGTTCGGCCATTTTAATTCAAGACCGGACCGAACCGACCACTTAGGGCCAAGCAAACACTAGACGCCACAGGAGCCCATCCAACATTGTTGGGCCAGGTAGCCATTTCTGAACCATAGATTTTCTTTTTTCTTTTTCTCTCGCCTCACCTGCAATTTGCGAAGGACCTCTGATTCTGCATCCTATCTCTGAGCTTCAACAGGTTATTTGACCCTTCTCCGCGCCTCTCCTGCTTGCCCTGCGCCGATTATTCCATTGGATCGCGTTGTTTAGCTGTTTCGNCATCCTATCTCTGAGCTTCAACAGGTTATTTGACCCTTCTCCGCGCCTCTCCTGCTTGCCCTGCGCCGATTATTCCATTGGATCGCGTTGTTTAGCTGAAGTGTGTATTTTTGCTTCTGCTTTGTTGCCCGATTTCTCCACGGATCTATGCCTGTTCTTCCTCTTTGTTTTCGCTTTTCGGGCTAGGTTTTATATGGCGTGTCATGCCGTTTTTTAGATTTTGGTTGTAGTTTCAATTTTGTGACTTTTTAAGCTGTTATGATATTGATTGAAAGGCTAATTATGTTATTTCCGTACTGCGTAGAAGTATTTGTTCCATGACATGGGAGTTTTGTTTTGGTATGGATGTTGATTCATGTGAAATGGGGTTTGAAGCGTTAACAGATTGTCAACATGGTTAAAAAAATTTGATTTTGTGTTTATTTCTACCAGCATTTTTAATGGCATTTTCTGTAATTAGCTGGAATTTTCGTATTAGGTAGTATGCGTTCACTTTTTCCCTTTATCTTCGTTGTTGTAAGTTATCAGTTATGTGAGTTCTGGGTGATGTGTCTTGTATAGTAATCTTCTCCTCTCAACTCTCTGCCATTATTGAAACAGTGTATGTGTTTTATCTTGTCCATTTTGACTCTTATTATTATTATTATTTTTTTTTTTTTGAATAATTTGCAGAAATTGTGACTGGAATGGCTGGGGGAGGAATGATCCGGCACCTACTCAGGAAATTTCCATCTCAATCTTCAGTAAGTACTTTAGCTTGTTATTCTATGTCCTAAACACAAAGGATAAGCTTTTGATTAAGTTCTCTATTTGTCCTGTCATAAATAGATGTTAAAGAATCTGCATAGTTAGTCACTATAATGCTTGATTTTGGGAAACAGTGTTGGTGTCACAAGTATTTACATTGTTGAAGAGGTGACTTTTACCGTAGTTATGGGAGATGAAGAGGACTGTTTCTATTTCATTGATCAGTGACATCAATTAAATTAGCTGATACATAGTTGTGGTTACGGTACCTTACTTGTGCAGACCCCTCCTTTGACCTCTTCCCTTATTTCAAAAAACGAGGAAGCTGGCTTTGCTGGGATGAACTCCTTTAGAAGGCTTGCGCTACTTGGAGCAGGCATTTCTGGATTTTTCAGTTTTGCAACTTTGGCATCTGCTGATGAGGCAGAACATGGCTTGGAGTGTCCAAACTATCCCTGGCCTCACCAGGGCATTTTGAGTTCATATGATCATGCTTCGTAAGATATGATGATGACTTTTTCTTTTTCCCTGCTTTATAGAACTGTCGGAATTCTTGAGCCGTCTTTACTTCACCATCAGTCTCACAGCTCTTACCTTAGTGTTTTAAGCCCGCTATAAGATGTTCTACATGTTTTTAATGATTTTTATTTTCTTTTTAGTTGATTTAGATGGATTATATTATTGTAGAGTGCAGCAATTTATTATAGCATTCCCCTGTTTTGACATGAAGTTGACTGCTCATTCGTGCTCGTCACTTATCATTGACCCATCTAGAAATTGATAGATGGCTCATATCTTAATGAGGTGATGGCCTTCTTATGTCTTTTGTCTAGAAGTAGATGGCCCATTCAATCCCGTCCTTGGTCATTGAAATATTAAGAAACTTTATATTAATGGACAGCCTCCATAGATTGTGCATAGTCTTATCATCTTCATCATCATCTTATCCTTCTTGTTATTGATGTTGTTATTANTTTTTTTTTTTTTTTTTTTTTCCCAATTTACATTTATTAAGGTGGAATTGTGATGACATAAAAGCATGAAAAAAGAAAAAGACTGGAAGGAGATCTGCAAAGGGAGCCCAGAATTAAATAAGATTCCAACAATCAAAGAATGGGAAGAGTAAATTCTATAATTTCTTCTTCAGCAGGAAAGGATTACAAAAAGTTATTCTTATGAAATAGAAGAGAAATCACTAGGAATTGTCCCAAGGCATGGAAGATTATAAAACTCTTCCATTTGGTGTTTATCGAGAAGGAACTGTAATTGCCAAGAGAGATTAGAAAAAAAGCTTCGAAGTGAGCCCAGATTTTGATTATATCCCAAATTTCAAAGAAAGGGTGGAGAAATTATATAAATCTCTTCCAATTAGCATTTTAGTTTTGAACCCTGAAAATGTTTAAGAAGGGGGAGATAAGGAATCGTCTCTTCAAAGGATTACTAATAGTTCTTTCATTGGGTTCATATCAAGAAGGAACTGTAATTAGAAAAGAAATTGAGAAGAAAGACTATTTAGAGAGCCCGTAATTTAATGAGATCCTAATCATCCTAGAAGAGTCAGCAAAAATTCTACAATTTCTTTCCAATTAAACCTATCAAGGCAAGGCTAGCCAGAATAACATTCTCCCTTAGATCTTTGCCCATATATAGGGGTTTCAAAGAGTTAAAAACTTTTCCTGGTAAGCACCAGGTGAGACTAAAATCCTTTTAAATTCCTTGCCAACATTTCAAAGTGAAGGAGCATGTCACATTTTCTCTTATCCATTAATTTGTTTAACCCTATGGTCTTGTGGATAACATATTTCCTATCTGTTCCTCCTGACTCAATAAATTAATTGGTCAACATTAATCATTTATGTTGAGAGAGGTTTGATGATAGTGTATAATCTTAATGCTCTAAGGTTAATTTTGGTTGAGCAGGATTAGGCGTGGTCATCAGGTTTATCAACAAGTGTGTGCATCTTGTCATTCCATGTCTTTGATATCATATCGTGATTTGGTTGGTGTTGCATATACTGAAGAGGAGACGAAAGCAATGGCTGCTGAAATTGAGGTAGTGGATGGGCCTAATGATGAGGGTGAGATGTTCACTCGTCCAGGTAAACTCAGTGATCGGTTTCCTGAACCATATGCCAATGAACAAGCTGCTAGATTTGCTAATGGTGGAGCTTACCCTCCGGATCTAAGCCTCATCACAAAGGTAATTCTTCTTTATCATTCTTGTAGTTTCAGGAAGCAAGATTGTATATATTAAATCATGGGTGTTGACTATTGATACCAAAATGTTTATCCGTTCCTTTTCCCCTTGGTGATACAAACAGAATCATTTATGCATATAATGTAGAGAATTTGTGAATAAACTTTGTTCTAATGCCATTTTATCTTTTTCCCTCTCAGGCTCGTCATAATGGTCAGAATTATGTATTTTCCCTTCTGACTGGTTATCGTGACCCACCTGCTGGTGTTTCGGTAAATTTTAATCCACATTATTTTTACTAAATTCCATAGGATGTTCTGTATTTCTCATTGCAAATTTACAGTTGTAAATTATGGGACTTCTGTGAGTAGAAGATATATTTTGATTGTCCTTATTAGGATACTACCATGTTCTATTATTTCATGTGAGCTTCCTTCAGTAGGGTTAGTTAGTTTTAGATGTCTTCTATTGCCTGCCAATTTTGATGTAATGTGTGGGTACAGTTCATATGGAACAGATATGTGCATGTTGCATATCTTAGTTGAAATTAATATAACACCATATTTGTTTTTTTTAGTGTGCGAGATAGACGTTTCTTGTGGCCTCAGAAGCTGCAAGTAGAGTGTCATATTTTCGTGCTTATTTATGGTAGAAAATTGTAACTCACCTCTGAGGTCTAGTTTGAACTTAGATAGCCTGTTTTGGAAGGTGTATTTCTTCATTGTGTGAAATCATCAGATGTCTTGCATATTGATTTTGGTTTTGTTTGTTACTCCCCTGCCTCATTGGAGTCTGATGGAGTATCTTTTATCCATCTGAGTATTCTATTCTTGAATAAATATGAAACAACCTTTTATTCTTATGATCATTGTTAAACATTAGTTCACAGTGTTGTACTCGATTTGAAGTGAGCCATGGTCATTTAAAGCTTAGAATTGGACGAAATTCAAATATTTCCAACCAATAAGCAAATTTGTATTATTTTCCAGCTTGATAACTCTTCTCAATTCTGAAGAACACTCTGAATGGTTTTGTAGATCCGAGAGGGTCTGCACTATAACCCTTATTTCCCTGGTGGAGCAATTGCAATGCCTAAGATGCTTAATGATGGAGCTGTTGAATACGAAGATGGTACCCCTGCAACAGAAGCACAGGTTAGTCTGCAAAATATGCAAAGTTCGTAAGTTGTATAGAACATGAGAATATGTATTACGAATGCACAAATCTACCAGCGCTGACTGAGCATCATATTGCTCAAGTATTGATCCTTCGTCCATCGACTTATAAGTTCCATGCTCCTTTGTATTGTAGATGGGGAAAGATGTTGTGTCATTCTTGTCATGGGCAGCTGAACCAGAAATGGAAGAAAGGAAACTGGTAATTTGCATATTAAATCTATGTTGCCTTGTTTCACGTTTCTAGTTCTCTTGTCTGATGTGTGTTTGGCCTTGAATGGCAGATGGGATTCAAATGGATCTTCGTGCTCTCTTTGGCTCTACTTCAGGCCGCATACTACCGCCGATTGAAGTGGTCAGTTTTGAAGTCCCGCAAGCTGGTTCTTGATGTAGTCAACTAGTAGCTACCTGCATTTGAGTTTGTGTCTATTATTTTGCTTCTGGTAAGAAATGAATTGGCAATAAGTATCTGTTAGAATGTAATGGTTCTTGCGAAGCAATTTCCTTTTTGAATCCGTTAATTTGCAGTGAAATGACCTGCATGTGTCGAGTTCCTCCTTTTTGATGAAAATAAATTCATTAGTTTGTGGTAAATTGTGAGTAAGATTTATAGGCCAAAACAAGGAATTCGAAGGGTGTCTGCCTATTTGGAGTTAACAATTATGTTTTACCAAATGACCACATTTTGGCCTATTCAGTGATTAGTTTATAT

mRNA sequence

TTTCATACATATAGATGAAAAAATATGGTAAAATTTACTGATTTGTTAGAATAAAATTAAATTTATATCTAATAATTAATTGATTTAAAAATNTTTATCAAACAAATTTTGAGATAAAAATTAATTCTTTTTTCACACATATAGATTAAATTTATATCTAATAATTAATTGATTTAAAATTTTAGCTCTTGAAAATTGCCGGTTTTTGCAGAATTGGTTCGGCCATTTTAATTCAAGACCGGACCGAACCGACCACTTAGGGCCAAGCAAACACTAGACGCCACAGGAGCCCATCCAACATTGTTGGGCCAGGTAGCCATTTCTGAACCATAGATTTTCTTTTTTCTTTTTCTCTCGCCTCACCTGCAATTTGCGAAGGACCTCTGATTCTGCATCCTATCTCTGAGCTTCAACAGAAATTGTGACTGGAATGGCTGGGGGAGGAATGATCCGGCACCTACTCAGGAAATTTCCATCTCAATCTTCAACCCCTCCTTTGACCTCTTCCCTTATTTCAAAAAACGAGGAAGCTGGCTTTGCTGGGATGAACTCCTTTAGAAGGCTTGCGCTACTTGGAGCAGGCATTTCTGGATTTTTCAGTTTTGCAACTTTGGCATCTGCTGATGAGGCAGAACATGGCTTGGAGTGTCCAAACTATCCCTGGCCTCACCAGGGCATTTTGAGTTCATATGATCATGCTTCGATTAGGCGTGGTCATCAGGTTTATCAACAAGTGTGTGCATCTTGTCATTCCATGTCTTTGATATCATATCGTGATTTGGTTGGTGTTGCATATACTGAAGAGGAGACGAAAGCAATGGCTGCTGAAATTGAGGTAGTGGATGGGCCTAATGATGAGGGTGAGATGTTCACTCGTCCAGGTAAACTCAGTGATCGGTTTCCTGAACCATATGCCAATGAACAAGCTGCTAGATTTGCTAATGGTGGAGCTTACCCTCCGGATCTAAGCCTCATCACAAAGGCTCGTCATAATGGTCAGAATTATGTATTTTCCCTTCTGACTGGTTATCGTGACCCACCTGCTGGTGTTTCGATCCGAGAGGGTCTGCACTATAACCCTTATTTCCCTGGTGGAGCAATTGCAATGCCTAAGATGCTTAATGATGGAGCTGTTGAATACGAAGATGGTACCCCTGCAACAGAAGCACAGATGGGGAAAGATGTTGTGTCATTCTTGTCATGGGCAGCTGAACCAGAAATGGAAGAAAGGAAACTGATGGGATTCAAATGGATCTTCGTGCTCTCTTTGGCTCTACTTCAGGCCGCATACTACCGCCGATTGAAGTGGTCAGTTTTGAAGTCCCGCAAGCTGGTTCTTGATGTAGTCAACTAGTAGCTACCTGCATTTGAGTTTGTGTCTATTATTTTGCTTCTGGTAAGAAATGAATTGGCAATAAGTATCTGTTAGAATGTAATGGTTCTTGCGAAGCAATTTCCTTTTTGAATCCGTTAATTTGCAGTGAAATGACCTGCATGTGTCGAGTTCCTCCTTTTTGATGAAAATAAATTCATTAGTTTGTGGTAAATTGTGAGTAAGATTTATAGGCCAAAACAAGGAATTCGAAGGGTGTCTGCCTATTTGGAGTTAACAATTATGTTTTACCAAATGACCACATTTTGGCCTATTCAGTGATTAGTTTATAT

Coding sequence (CDS)

ATGGCTGGGGGAGGAATGATCCGGCACCTACTCAGGAAATTTCCATCTCAATCTTCAACCCCTCCTTTGACCTCTTCCCTTATTTCAAAAAACGAGGAAGCTGGCTTTGCTGGGATGAACTCCTTTAGAAGGCTTGCGCTACTTGGAGCAGGCATTTCTGGATTTTTCAGTTTTGCAACTTTGGCATCTGCTGATGAGGCAGAACATGGCTTGGAGTGTCCAAACTATCCCTGGCCTCACCAGGGCATTTTGAGTTCATATGATCATGCTTCGATTAGGCGTGGTCATCAGGTTTATCAACAAGTGTGTGCATCTTGTCATTCCATGTCTTTGATATCATATCGTGATTTGGTTGGTGTTGCATATACTGAAGAGGAGACGAAAGCAATGGCTGCTGAAATTGAGGTAGTGGATGGGCCTAATGATGAGGGTGAGATGTTCACTCGTCCAGGTAAACTCAGTGATCGGTTTCCTGAACCATATGCCAATGAACAAGCTGCTAGATTTGCTAATGGTGGAGCTTACCCTCCGGATCTAAGCCTCATCACAAAGGCTCGTCATAATGGTCAGAATTATGTATTTTCCCTTCTGACTGGTTATCGTGACCCACCTGCTGGTGTTTCGATCCGAGAGGGTCTGCACTATAACCCTTATTTCCCTGGTGGAGCAATTGCAATGCCTAAGATGCTTAATGATGGAGCTGTTGAATACGAAGATGGTACCCCTGCAACAGAAGCACAGATGGGGAAAGATGTTGTGTCATTCTTGTCATGGGCAGCTGAACCAGAAATGGAAGAAAGGAAACTGATGGGATTCAAATGGATCTTCGTGCTCTCTTTGGCTCTACTTCAGGCCGCATACTACCGCCGATTGAAGTGGTCAGTTTTGAAGTCCCGCAAGCTGGTTCTTGATGTAGTCAACTAG

Protein sequence

MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRKLVLDVVN
Homology
BLAST of Cp4.1LG01g08870 vs. ExPASy Swiss-Prot
Match: Q9FKS5 (Cytochrome c1 2, heme protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CYC1-2 PE=1 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-153
Identity = 271/310 (87.42%), Postives = 286/310 (92.26%), Query Frame = 0

Query: 1   MAGGGMIRHLL-RKFPSQSSTPPLTSSLISK--NEEAGFAGMNSFRRLALLGAGISGFFS 60
           M GGG+IR LL RK  SQS   P+ S L SK  NE+AG AG+ +F   AL+GAGI+G  S
Sbjct: 1   MVGGGVIRQLLRRKLHSQSVATPVLSWLSSKKANEDAGSAGLRAF---ALMGAGITGLLS 60

Query: 61  FATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120
           F+T+ASADEAEHGLECPNYPWPH+GILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL
Sbjct: 61  FSTVASADEAEHGLECPNYPWPHEGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120

Query: 121 VGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPP 180
           VGVAYTEEE KAMAAEIEVVDGPNDEGEMFTRPGKLSDR PEPY+NE AARFANGGAYPP
Sbjct: 121 VGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRLPEPYSNESAARFANGGAYPP 180

Query: 181 DLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEY 240
           DLSL+TKARHNGQNYVF+LLTGYRDPPAG+SIREGLHYNPYFPGGAIAMPKMLND AVEY
Sbjct: 181 DLSLVTKARHNGQNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMPKMLNDEAVEY 240

Query: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLK 300
           EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIF+LSLALLQAAYYRRLKWSVLK
Sbjct: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFLLSLALLQAAYYRRLKWSVLK 300

Query: 301 SRKLVLDVVN 308
           SRKLVLDVVN
Sbjct: 301 SRKLVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy Swiss-Prot
Match: Q9LK29 (Cytochrome c1 1, heme protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CYC1-1 PE=1 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 1.9e-148
Identity = 263/310 (84.84%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 1   MAGGGMIRHLL-RKFPSQSSTPPLTSSLISK--NEEAGFAGMNSFRRLALLGAGISGFFS 60
           M GGG+I+ +L RK  SQS   P+ S   SK  +E+AG +G+   R LALLGAG++G  S
Sbjct: 1   MVGGGVIQQILRRKLHSQSLATPVLSWFSSKKAHEDAGSSGV---RALALLGAGVTGLLS 60

Query: 61  FATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120
           F+T+ASADEAEHGLE P YPWPH GILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL
Sbjct: 61  FSTVASADEAEHGLESPEYPWPHDGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120

Query: 121 VGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPP 180
           VGVAYTEEE KAMAAEIEVVDGPNDEGEMFTRPGKLSDRFP+PYANE AARFANGGAYPP
Sbjct: 121 VGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPQPYANESAARFANGGAYPP 180

Query: 181 DLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEY 240
           DLSLITKARHNG NYVF+LLTGYRDPPAG+SIREGLHYNPYFPGGAIAMPKMLND AVEY
Sbjct: 181 DLSLITKARHNGPNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMPKMLNDEAVEY 240

Query: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLK 300
           EDG PATEAQMGKD+VSFL+WAAEPEMEERKLMGFKWIF+LSLALLQAAYYRRLKWSVLK
Sbjct: 241 EDGVPATEAQMGKDIVSFLAWAAEPEMEERKLMGFKWIFLLSLALLQAAYYRRLKWSVLK 300

Query: 301 SRKLVLDVVN 308
           SRKLVLDVVN
Sbjct: 301 SRKLVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy Swiss-Prot
Match: P25076 (Cytochrome c1-1, heme protein, mitochondrial OS=Solanum tuberosum OX=4113 GN=CYCL PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.5e-148
Identity = 261/308 (84.74%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 3   GGGMIRHLLRKFPSQ---SSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFA 62
           G G I   + +  +Q   S  P    +L    +  G AG  SFR LA +GAG+SG  SFA
Sbjct: 13  GFGRINRFITRGAAQRNDSKLPSRNDALKHGLDGLGSAGSKSFRALAAIGAGVSGLLSFA 72

Query: 63  TLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVG 122
           T+A +DEAEHGLECPNYPWPH+GILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVG
Sbjct: 73  TIAYSDEAEHGLECPNYPWPHEGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVG 132

Query: 123 VAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDL 182
           VAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFP+PYANE AARFANGGAYPPDL
Sbjct: 133 VAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPQPYANEAAARFANGGAYPPDL 192

Query: 183 SLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYED 242
           SLITKARHNGQNYVF+LLT YRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYED
Sbjct: 193 SLITKARHNGQNYVFALLTAYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYED 252

Query: 243 GTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSR 302
           G PATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRL+WSVLKSR
Sbjct: 253 GIPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLRWSVLKSR 312

Query: 303 KLVLDVVN 308
           KLVLDVVN
Sbjct: 313 KLVLDVVN 320

BLAST of Cp4.1LG01g08870 vs. ExPASy Swiss-Prot
Match: P29610 (Cytochrome c1-2, heme protein, mitochondrial (Fragment) OS=Solanum tuberosum OX=4113 GN=CYCL PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 3.0e-141
Identity = 241/260 (92.69%), Postives = 250/260 (96.15%), Query Frame = 0

Query: 48  LGAGISGFFSFATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCH 107
           +GAG+SG   FAT+ASADEAEHGLECP+YPWPH GILSSYDHASIRRGHQVYQQVCASCH
Sbjct: 1   IGAGVSGLLGFATVASADEAEHGLECPSYPWPHAGILSSYDHASIRRGHQVYQQVCASCH 60

Query: 108 SMSLISYRDLVGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAA 167
           SMSL+SYRDLVGVAYTEEE KAMAAEIEV DGPNDEGEMFTRPGKLSDRFP+PY NE AA
Sbjct: 61  SMSLVSYRDLVGVAYTEEEVKAMAAEIEVEDGPNDEGEMFTRPGKLSDRFPQPYPNEAAA 120

Query: 168 RFANGGAYPPDLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMP 227
           RFANGGAYPPDLSLITKARHNGQNYVF+LLT YRDPPAGVSIREGLHYNPYFPGGAIAMP
Sbjct: 121 RFANGGAYPPDLSLITKARHNGQNYVFALLTAYRDPPAGVSIREGLHYNPYFPGGAIAMP 180

Query: 228 KMLNDGAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAY 287
           KMLNDGAVEYEDG PATEAQMGKDVVSFL+WAAEPEMEERKLMGFKWIFVLSLALLQAAY
Sbjct: 181 KMLNDGAVEYEDGVPATEAQMGKDVVSFLTWAAEPEMEERKLMGFKWIFVLSLALLQAAY 240

Query: 288 YRRLKWSVLKSRKLVLDVVN 308
           YRRL+WSVLKSRKLVLDVVN
Sbjct: 241 YRRLRWSVLKSRKLVLDVVN 260

BLAST of Cp4.1LG01g08870 vs. ExPASy Swiss-Prot
Match: Q9D0M3 (Cytochrome c1, heme protein, mitochondrial OS=Mus musculus OX=10090 GN=Cyc1 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-84
Identity = 153/257 (59.53%), Postives = 191/257 (74.32%), Query Frame = 0

Query: 45  LALLGAGISGFFSFATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCA 104
           L +L AG +G  + A  ++   ++  L  P+YPW H+G+LSS DH SIRRG QVY+QVC+
Sbjct: 64  LGMLAAGGAG-LAVALHSAVSASDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCS 123

Query: 105 SCHSMSLISYRDLVGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANE 164
           SCHSM  ++YR LVGV YTEEE KA+A E+EV DGPND+GEMF RPGKLSD FP+PY N 
Sbjct: 124 SCHSMDYVAYRHLVGVCYTEEEAKALAEEVEVQDGPNDDGEMFMRPGKLSDYFPKPYPNP 183

Query: 165 QAARFANGGAYPPDLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAI 224
           +AAR AN GA PPDLS I +ARH G++YVFSLLTGY +PP GVS+REGL++NPYFPG AI
Sbjct: 184 EAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAI 243

Query: 225 AMPKMLNDGAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQ 284
            M   +    +EY+DGTPAT +Q+ KDV +FL WA+EPE + RK MG K + ++ L L  
Sbjct: 244 GMAPPIYTEVLEYDDGTPATMSQVAKDVATFLRWASEPEHDHRKRMGLKMLLMMGLLLPL 303

Query: 285 AAYYRRLKWSVLKSRKL 302
               +R KWSVLKSRKL
Sbjct: 304 TYAMKRHKWSVLKSRKL 319

BLAST of Cp4.1LG01g08870 vs. NCBI nr
Match: XP_022942776.1 (cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita moschata] >XP_022942777.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita moschata] >XP_022942778.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita moschata] >XP_022942779.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita moschata] >XP_022991856.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita maxima] >XP_022991867.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita maxima] >XP_022991875.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita maxima] >XP_022991884.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita maxima] >XP_023547377.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023547384.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023547391.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023547400.1 cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG7031321.1 Cytochrome c1-1, heme protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620 bits (1599), Expect = 4.41e-224
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. NCBI nr
Match: KAG6600686.1 (Cytochrome c1-1, heme protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620 bits (1599), Expect = 1.21e-223
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 28  MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 87

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 88  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 147

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 148 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 207

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 208 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 267

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 268 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 327

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 328 LVLDVVN 334

BLAST of Cp4.1LG01g08870 vs. NCBI nr
Match: XP_022984788.1 (cytochrome c1-2, heme protein, mitochondrial [Cucurbita maxima] >XP_022984789.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita maxima] >XP_022984790.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita maxima])

HSP 1 Score: 616 bits (1589), Expect = 1.48e-222
Identity = 305/307 (99.35%), Postives = 306/307 (99.67%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           +ASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  IASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. NCBI nr
Match: XP_022929464.1 (cytochrome c1-2, heme protein, mitochondrial [Cucurbita moschata] >XP_022929465.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita moschata] >XP_023552741.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023552742.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023552743.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023552744.1 cytochrome c1-2, heme protein, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615 bits (1586), Expect = 4.23e-222
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           +ASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  IASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVF+LLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFALLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. NCBI nr
Match: KAG7015421.1 (Cytochrome c1-1, heme protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 612 bits (1578), Expect = 7.01e-221
Identity = 303/307 (98.70%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPL SSLISKNEEA FAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLASSLISKNEEASFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           +ASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  IASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy TrEMBL
Match: A0A6J1JN34 (cytochrome c1-2, heme protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111488375 PE=4 SV=1)

HSP 1 Score: 620 bits (1599), Expect = 2.13e-224
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy TrEMBL
Match: A0A6J1FPU6 (cytochrome c1-2, heme protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111447710 PE=4 SV=1)

HSP 1 Score: 620 bits (1599), Expect = 2.13e-224
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy TrEMBL
Match: A0A6J1J9J9 (cytochrome c1-2, heme protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111482970 PE=4 SV=1)

HSP 1 Score: 616 bits (1589), Expect = 7.14e-223
Identity = 305/307 (99.35%), Postives = 306/307 (99.67%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           +ASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  IASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy TrEMBL
Match: A0A6J1EUG7 (cytochrome c1-2, heme protein, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111436032 PE=4 SV=1)

HSP 1 Score: 615 bits (1586), Expect = 2.05e-222
Identity = 304/307 (99.02%), Postives = 306/307 (99.67%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           +ASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  IASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVF+LLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFALLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. ExPASy TrEMBL
Match: A0A6J1C806 (cytochrome c1-2, heme protein, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111008263 PE=4 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 1.38e-220
Identity = 301/307 (98.05%), Postives = 305/307 (99.35%), Query Frame = 0

Query: 1   MAGGGMIRHLLRKFPSQSSTPPLTSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60
           MAGGGMIR LLRKFPSQSSTPPL+SSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT
Sbjct: 1   MAGGGMIRQLLRKFPSQSSTPPLSSSLISKNEEAGFAGMNSFRRLALLGAGISGFFSFAT 60

Query: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120
           LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV
Sbjct: 61  LASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGV 120

Query: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPPDLS 180
           AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFP+PYANEQAARFANGGAYPPDLS
Sbjct: 121 AYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPQPYANEQAARFANGGAYPPDLS 180

Query: 181 LITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240
           LITKARHNGQNYVF+LLTGYRDPPAGVSIR+GLHYNPYFPGGAIAMPKMLNDGAVEYEDG
Sbjct: 181 LITKARHNGQNYVFALLTGYRDPPAGVSIRDGLHYNPYFPGGAIAMPKMLNDGAVEYEDG 240

Query: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLKSRK 300
           TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQA YYRRLKWSVLKSRK
Sbjct: 241 TPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAGYYRRLKWSVLKSRK 300

Query: 301 LVLDVVN 307
           LVLDVVN
Sbjct: 301 LVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. TAIR 10
Match: AT5G40810.1 (Cytochrome C1 family )

HSP 1 Score: 542.7 bits (1397), Expect = 1.9e-154
Identity = 271/310 (87.42%), Postives = 286/310 (92.26%), Query Frame = 0

Query: 1   MAGGGMIRHLL-RKFPSQSSTPPLTSSLISK--NEEAGFAGMNSFRRLALLGAGISGFFS 60
           M GGG+IR LL RK  SQS   P+ S L SK  NE+AG AG+ +F   AL+GAGI+G  S
Sbjct: 1   MVGGGVIRQLLRRKLHSQSVATPVLSWLSSKKANEDAGSAGLRAF---ALMGAGITGLLS 60

Query: 61  FATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120
           F+T+ASADEAEHGLECPNYPWPH+GILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL
Sbjct: 61  FSTVASADEAEHGLECPNYPWPHEGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120

Query: 121 VGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPP 180
           VGVAYTEEE KAMAAEIEVVDGPNDEGEMFTRPGKLSDR PEPY+NE AARFANGGAYPP
Sbjct: 121 VGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRLPEPYSNESAARFANGGAYPP 180

Query: 181 DLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEY 240
           DLSL+TKARHNGQNYVF+LLTGYRDPPAG+SIREGLHYNPYFPGGAIAMPKMLND AVEY
Sbjct: 181 DLSLVTKARHNGQNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMPKMLNDEAVEY 240

Query: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLK 300
           EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIF+LSLALLQAAYYRRLKWSVLK
Sbjct: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFLLSLALLQAAYYRRLKWSVLK 300

Query: 301 SRKLVLDVVN 308
           SRKLVLDVVN
Sbjct: 301 SRKLVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. TAIR 10
Match: AT3G27240.1 (Cytochrome C1 family )

HSP 1 Score: 526.6 bits (1355), Expect = 1.4e-149
Identity = 263/310 (84.84%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 1   MAGGGMIRHLL-RKFPSQSSTPPLTSSLISK--NEEAGFAGMNSFRRLALLGAGISGFFS 60
           M GGG+I+ +L RK  SQS   P+ S   SK  +E+AG +G+   R LALLGAG++G  S
Sbjct: 1   MVGGGVIQQILRRKLHSQSLATPVLSWFSSKKAHEDAGSSGV---RALALLGAGVTGLLS 60

Query: 61  FATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120
           F+T+ASADEAEHGLE P YPWPH GILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL
Sbjct: 61  FSTVASADEAEHGLESPEYPWPHDGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDL 120

Query: 121 VGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAARFANGGAYPP 180
           VGVAYTEEE KAMAAEIEVVDGPNDEGEMFTRPGKLSDRFP+PYANE AARFANGGAYPP
Sbjct: 121 VGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPQPYANESAARFANGGAYPP 180

Query: 181 DLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMPKMLNDGAVEY 240
           DLSLITKARHNG NYVF+LLTGYRDPPAG+SIREGLHYNPYFPGGAIAMPKMLND AVEY
Sbjct: 181 DLSLITKARHNGPNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMPKMLNDEAVEY 240

Query: 241 EDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLKWSVLK 300
           EDG PATEAQMGKD+VSFL+WAAEPEMEERKLMGFKWIF+LSLALLQAAYYRRLKWSVLK
Sbjct: 241 EDGVPATEAQMGKDIVSFLAWAAEPEMEERKLMGFKWIFLLSLALLQAAYYRRLKWSVLK 300

Query: 301 SRKLVLDVVN 308
           SRKLVLDVVN
Sbjct: 301 SRKLVLDVVN 307

BLAST of Cp4.1LG01g08870 vs. TAIR 10
Match: AT5G40810.2 (Cytochrome C1 family )

HSP 1 Score: 509.6 bits (1311), Expect = 1.7e-144
Identity = 244/260 (93.85%), Postives = 254/260 (97.69%), Query Frame = 0

Query: 48  LGAGISGFFSFATLASADEAEHGLECPNYPWPHQGILSSYDHASIRRGHQVYQQVCASCH 107
           +GAGI+G  SF+T+ASADEAEHGLECPNYPWPH+GILSSYDHASIRRGHQVYQQVCASCH
Sbjct: 1   MGAGITGLLSFSTVASADEAEHGLECPNYPWPHEGILSSYDHASIRRGHQVYQQVCASCH 60

Query: 108 SMSLISYRDLVGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPEPYANEQAA 167
           SMSLISYRDLVGVAYTEEE KAMAAEIEVVDGPNDEGEMFTRPGKLSDR PEPY+NE AA
Sbjct: 61  SMSLISYRDLVGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRLPEPYSNESAA 120

Query: 168 RFANGGAYPPDLSLITKARHNGQNYVFSLLTGYRDPPAGVSIREGLHYNPYFPGGAIAMP 227
           RFANGGAYPPDLSL+TKARHNGQNYVF+LLTGYRDPPAG+SIREGLHYNPYFPGGAIAMP
Sbjct: 121 RFANGGAYPPDLSLVTKARHNGQNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMP 180

Query: 228 KMLNDGAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAY 287
           KMLND AVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIF+LSLALLQAAY
Sbjct: 181 KMLNDEAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFLLSLALLQAAY 240

Query: 288 YRRLKWSVLKSRKLVLDVVN 308
           YRRLKWSVLKSRKLVLDVVN
Sbjct: 241 YRRLKWSVLKSRKLVLDVVN 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKS52.6e-15387.42Cytochrome c1 2, heme protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LK291.9e-14884.84Cytochrome c1 1, heme protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=... [more]
P250762.5e-14884.74Cytochrome c1-1, heme protein, mitochondrial OS=Solanum tuberosum OX=4113 GN=CYC... [more]
P296103.0e-14192.69Cytochrome c1-2, heme protein, mitochondrial (Fragment) OS=Solanum tuberosum OX=... [more]
Q9D0M31.2e-8459.53Cytochrome c1, heme protein, mitochondrial OS=Mus musculus OX=10090 GN=Cyc1 PE=1... [more]
Match NameE-valueIdentityDescription
XP_022942776.14.41e-224100.00cytochrome c1-2, heme protein, mitochondrial-like [Cucurbita moschata] >XP_02294... [more]
KAG6600686.11.21e-223100.00Cytochrome c1-1, heme protein, mitochondrial, partial [Cucurbita argyrosperma su... [more]
XP_022984788.11.48e-22299.35cytochrome c1-2, heme protein, mitochondrial [Cucurbita maxima] >XP_022984789.1 ... [more]
XP_022929464.14.23e-22299.02cytochrome c1-2, heme protein, mitochondrial [Cucurbita moschata] >XP_022929465.... [more]
KAG7015421.17.01e-22198.70Cytochrome c1-1, heme protein, mitochondrial, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
A0A6J1JN342.13e-224100.00cytochrome c1-2, heme protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1FPU62.13e-224100.00cytochrome c1-2, heme protein, mitochondrial-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1J9J97.14e-22399.35cytochrome c1-2, heme protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EUG72.05e-22299.02cytochrome c1-2, heme protein, mitochondrial OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1C8061.38e-22098.05cytochrome c1-2, heme protein, mitochondrial OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G40810.11.9e-15487.42Cytochrome C1 family [more]
AT3G27240.11.4e-14984.84Cytochrome C1 family [more]
AT5G40810.21.7e-14493.85Cytochrome C1 family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002326Cytochrome c1PRINTSPR00603CYTOCHROMEC1coord: 174..198
score: 60.0
coord: 212..223
score: 61.9
coord: 141..161
score: 55.78
coord: 262..277
score: 46.43
coord: 76..95
score: 50.0
coord: 223..242
score: 59.64
coord: 243..262
score: 65.0
coord: 96..115
score: 56.79
IPR002326Cytochrome c1PFAMPF02167Cytochrom_C1coord: 78..294
e-value: 1.1E-97
score: 326.1
IPR002326Cytochrome c1PANTHERPTHR10266CYTOCHROME C1coord: 25..307
NoneNo IPR availableGENE3D1.20.5.100coord: 263..306
e-value: 3.5E-17
score: 64.2
NoneNo IPR availablePANTHERPTHR10266:SF13CYTOCHROME C1-1, HEME PROTEIN, MITOCHONDRIALcoord: 25..307
IPR036909Cytochrome c-like domain superfamilyGENE3D1.10.760.10coord: 67..262
e-value: 7.9E-92
score: 308.8
IPR036909Cytochrome c-like domain superfamilySUPERFAMILY46626Cytochrome ccoord: 71..261
IPR009056Cytochrome c-like domainPROSITEPS51007CYTCcoord: 90..197
score: 7.389183
IPR021157Cytochrome c1, transmembrane anchor, C-terminalSUPERFAMILY81496Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchorcoord: 262..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g08870.1Cp4.1LG01g08870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c
cellular_component GO:0005750 mitochondrial respiratory chain complex III
molecular_function GO:0009055 electron transfer activity
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding