Cp4.1LG01g08770 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g08770 vs. ExPASy Swiss-Prot
Match: Q3KTM0 (Ferric reduction oxidase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FRO7 PE=2 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 7.0e-224
Identity = 418/758 (55.15%), Postives = 528/758 (69.66%), Query Frame = 0

Query: 37  ATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGASGQTLFGITGA 96
           ++ ++S +VSS KW+LK+ M VIF  W+V + +YP S    + + W   S  TLFG+TG+
Sbjct: 20  SSSSSSVVVSSLKWILKVVMSVIFVTWVVFLMMYPGSLGDQILTNWRAISSNTLFGLTGS 79

Query: 97  IFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKN 156
           +FL FS P+L IAILA++YLIIS ++  +                            KK 
Sbjct: 80  MFLIFSGPILVIAILASLYLIISGEETVF---------------------------TKKK 139

Query: 157 GKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTF 216
             + PR+RLWT PVLV+GP  VV+AAE +GI+V   +  WAIY+YTLR L      ++  
Sbjct: 140 ITKFPRFRLWTFPVLVDGPFGVVSAAEFLGIMVFSVFFLWAIYAYTLRNLNVLDYFHVLP 199

Query: 217 LQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTML 276
              S  +LE+ GLR G +GL C+ FLFLPISRGS+LLRLIDIPFEHATRYHVWLGH+TM 
Sbjct: 200 NNRSIFLLELTGLRFGMIGLLCMVFLFLPISRGSILLRLIDIPFEHATRYHVWLGHITMT 259

Query: 277 IFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFE 336
            F+LHGL +V+ W IQG+ +E L EWK  GIA LPGVISL+AGLLMW+TSL  +R   FE
Sbjct: 260 FFSLHGLCYVVGWTIQGQLLELLFEWKATGIAVLPGVISLVAGLLMWVTSLHTVRKNYFE 319

Query: 337 LFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPC 396
           LFFYTHQLYIVFV+FLALHVGD++FSI AGGIF+F+LDRFLRF QSRRTVDVISAK+LPC
Sbjct: 320 LFFYTHQLYIVFVVFLALHVGDYLFSIVAGGIFLFILDRFLRFYQSRRTVDVISAKSLPC 379

Query: 397 GTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWT 456
           GT+EL++SKP ++RYNALSFIFLQV+ELS LQWHPFSVSSSPL+G   +A+LIKVLG WT
Sbjct: 380 GTLELVLSKPPNMRYNALSFIFLQVKELSWLQWHPFSVSSSPLDGNHHVAVLIKVLGGWT 439

Query: 457 EKLRGKIL----SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISP 516
            KLR ++     ++   ++ S +    +T  VEGPYGHESPYHL YENL+LVAGGIGI+P
Sbjct: 440 AKLRDQLSTLYEAENQDQLISPESYPKITTCVEGPYGHESPYHLAYENLVLVAGGIGITP 499

Query: 517 FLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN--------------EGEI 576
           F AILSDILHR RDGK CLP K+LVVWAIKNS EL LLS ++              E  I
Sbjct: 500 FFAILSDILHRKRDGKDCLPGKVLVVWAIKNSDELSLLSAIDIPSICHFFSKKLNLEIHI 559

Query: 577 QPSKVTSIC----------------PLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILV 636
             ++ +  C                P + GC+MSVLVGTGDN+WSGLY+I ST+G + ++
Sbjct: 560 YVTRQSEPCLEDGMVHKVVHPSVKTPWTNGCSMSVLVGTGDNIWSGLYLIISTIGFIAMI 619

Query: 637 SLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVN 696
           +L+D +YIN +NI  WWYKGLLF +CMVASV++FGGLVV  W  WE          N+  
Sbjct: 620 TLVDIFYINKYNITTWWYKGLLFVVCMVASVLIFGGLVVVFWHRWEHKTGEVEANGNDKV 679

Query: 697 DIEKVGEDIPKDTLAQ-KDLN-PKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLI 756
           D+   GE+    + A+ K L   +   N TTI YGTRP F EI ES+   WG VD+GV++
Sbjct: 680 DLN--GEETHNPSAAELKGLAIEEDVQNYTTIRYGTRPAFREIFESLNGKWGSVDVGVIV 739

Query: 757 CGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 759
           CGP+TLQ++VA+ IRSH+ + R  +HP+FHF+SHSFDL
Sbjct: 740 CGPATLQTTVAKEIRSHS-IWRSANHPLFHFNSHSFDL 747

BLAST of Cp4.1LG01g08770 vs. ExPASy Swiss-Prot
Match: Q8RWS6 (Ferric reduction oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=FRO6 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.8e-216
Identity = 413/759 (54.41%), Postives = 524/759 (69.04%), Query Frame = 0

Query: 37  ATGNTSAIVSSTKWVLKLAMWVIFSVWMV-VIFLYPSSFASHLFSEWFGASGQTLFGITG 96
           ++ ++S I SS KW+LK+ M +IF  W+V ++ +YP        +     S +T  G TG
Sbjct: 13  SSSSSSVITSSLKWILKVVMSMIFVTWVVFLMMMYPEQLGDDFLT---FVSSKTFLGTTG 72

Query: 97  AIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKK 156
            +FL FS P+L I++LA++YLIIS +D   ++   K+IS F                   
Sbjct: 73  TMFLIFSCPILVISVLASLYLIISGED---KVFTKKKISKF------------------- 132

Query: 157 NGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLT 216
                PR+RLWT PVLV+GP  VV+AAE +GI+V   +  W+IY+YTLR L      ++ 
Sbjct: 133 -----PRFRLWTFPVLVDGPFGVVSAAEFLGIMVFSVFFLWSIYAYTLRNLDLLERFHVL 192

Query: 217 FLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTM 276
               S  +LEV GLRLG +GL C+ FLFLPISRGS+LLRLIDIPFEHATRYHVWLGH+TM
Sbjct: 193 PKNRSILLLEVTGLRLGMIGLLCMVFLFLPISRGSILLRLIDIPFEHATRYHVWLGHITM 252

Query: 277 LIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINF 336
             F+LHGL +V+ W+IQG+ +E +  W  IGIA LPGVISL+AGLLMW+TSL  +R   F
Sbjct: 253 AFFSLHGLCYVVGWIIQGQLLELIFSWNAIGIAILPGVISLVAGLLMWVTSLHTVRKNYF 312

Query: 337 ELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALP 396
           ELFFYTHQLYIVF++FLALHVGD++FSI AGGIF+F+LDRFLRF QSRRTVDVISAK+LP
Sbjct: 313 ELFFYTHQLYIVFIVFLALHVGDYMFSIVAGGIFLFILDRFLRFCQSRRTVDVISAKSLP 372

Query: 397 CGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKW 456
           CGT+EL++SKP ++RYNALSFIFLQVRELS LQWHPFSVSSSPL+G   +A+LIKVLG W
Sbjct: 373 CGTLELVLSKPPNMRYNALSFIFLQVRELSWLQWHPFSVSSSPLDGNHHVAVLIKVLGGW 432

Query: 457 TEKLRGKIL----SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGIS 516
           T KLR ++     ++   ++ S +    +T  VEGPYGHESPYHL YENL+LVAGGIGI+
Sbjct: 433 TAKLRDQLSNLYEAENQDQLISPQSYPKITTCVEGPYGHESPYHLAYENLVLVAGGIGIT 492

Query: 517 PFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNEGEIQP--SKVTSI--- 576
           PF AILSDILHR RDGK CLP K+LVVWAIKNS EL LLS ++   I P  SK  ++   
Sbjct: 493 PFFAILSDILHRKRDGKACLPSKVLVVWAIKNSDELSLLSAIDIPSICPFFSKKLNLEIH 552

Query: 577 -------------------------CPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVIL 636
                                     P + GC+MSVLVGTGDN+WSGLY+I ST+G + +
Sbjct: 553 IYITRQSEPRLEDGMVHKVVHPSVKLPRTNGCSMSVLVGTGDNIWSGLYLIISTIGFISM 612

Query: 637 VSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNV 696
           ++L+D +YI  +NI  WWYKGLLF  CMVASV++FGGLVV  W  WE          N+ 
Sbjct: 613 ITLLDIFYIKRYNITTWWYKGLLFVGCMVASVLIFGGLVVVFWHRWEHKTGEVEANGNDK 672

Query: 697 NDIEKVGEDIPKDTLAQ-KDLN-PKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVL 756
            D+   GE+    + A+ K L   +   N TTI YGTRP F EI ES+   WG VD+GV+
Sbjct: 673 VDLN--GEETHNPSAAELKGLAIEEDVQNYTTIRYGTRPAFREIFESLNGKWGSVDVGVI 732

Query: 757 ICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 759
           +CGP+TLQ++VA+ IRSH+ + R  +HP+FHF+SHSFDL
Sbjct: 733 VCGPATLQTTVAKEIRSHS-IWRSANHPLFHFNSHSFDL 738

BLAST of Cp4.1LG01g08770 vs. ExPASy Swiss-Prot
Match: Q9LMM2 (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.1e-86
Identity = 225/732 (30.74%), Postives = 381/732 (52.05%), Query Frame = 0

Query: 51  VLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGAS-GQTL-FGITGAIFLTFSAPLLAI 110
           V+K  M VI    +V+  + P+S    ++     A  G+++ +G  G   L +  P++ +
Sbjct: 14  VIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILL 73

Query: 111 AILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTL 170
           A L  +YL + +                     +N   +   KK+ K G  +        
Sbjct: 74  AFLGCIYLHLKKST------------------TVNQFNSGVEKKRAKFGALR-------R 133

Query: 171 PVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISCEV----- 230
           P+LV GPL +VT  E++ + + +  + W++ +Y  R          TF+ ++ E      
Sbjct: 134 PMLVNGPLGIVTVTEVMFLTMFMALLLWSLANYMYR----------TFVNVTSESAATDG 193

Query: 231 -------LEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTML 290
                  L+++ +R+G +G  CL FLF P++RGS LL  + +  E + +YH+WLGHL M+
Sbjct: 194 NNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSLLAAVGLTSESSIKYHIWLGHLVMI 253

Query: 291 IFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFE 350
           IFT HGL + I W+ + + + ++LEW    ++NL G I+L+AGL+MW+T+ PK+R   FE
Sbjct: 254 IFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNLAGEIALVAGLMMWVTTYPKIRRRLFE 313

Query: 351 LFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPC 410
           +FFY+H LYIVF+LF   HVG     I   G +IF++DRFLRF+QSR  V ++SA+ LPC
Sbjct: 314 VFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYIFLVDRFLRFLQSRNNVKLVSARVLPC 373

Query: 411 GTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWT 470
            TVEL  SK   L Y+  S +F+ +  +S LQWHPF++ SS     + L+++IK  GKW+
Sbjct: 374 DTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWHPFTIISSSKLEPETLSVMIKSQGKWS 433

Query: 471 EKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAI 530
            KL   + S  + +I+       + VSVEGPYG  S   L +E+L++V+GG GI+PF++I
Sbjct: 434 TKLYDMLSSSSSDQINR------LAVSVEGPYGPSSTDFLRHESLVMVSGGSGITPFISI 493

Query: 531 LSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTL-----------------NEGEIQ 590
           + D+ +     K  +PK  L+  A KNSS+L +L  +                  +  + 
Sbjct: 494 VRDLFYMSSTHKCKIPKMTLIC-AFKNSSDLSMLDLILPTSGLTTDMASFVDIQIKAFVT 553

Query: 591 PSKVTSICPLSKGCNM-------------SVLVGTGDNVWSGLYVI--SSTVGLVILVSL 650
             + TS+   +   N+              +    G N W  L  I  SS +  ++++++
Sbjct: 554 REEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSWLCLAAILSSSFMIFIVIIAI 613

Query: 651 MDFYYINPF--NIKK--WWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNN 710
           +  Y+I+P   N +K  W YK L+ +L  ++  V+       LW+  +++ +      +N
Sbjct: 614 ITRYHIHPIDQNSEKYTWAYKSLI-YLVSISITVVTTSTAAMLWNK-KKYYAKNDQYVDN 673

Query: 711 VNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLI 733
           ++ +  + E  P+  ++Q          ST I YG RPN ++++  +  +     +G+L+
Sbjct: 674 LSPV--IIESSPQQLISQ----------STDIHYGERPNLNKLLVGLKGS----SVGILV 685

BLAST of Cp4.1LG01g08770 vs. ExPASy Swiss-Prot
Match: P92949 (Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-83
Identity = 237/763 (31.06%), Postives = 370/763 (48.49%), Query Frame = 0

Query: 49  KWVLKLAMWVIF----SVW-MVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSA 108
           K V K  M VIF     +W M+    Y + +  HL  + FG S  T FG TG     +  
Sbjct: 24  KGVTKFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIK-FGTS--TYFGATGTTLFMYMF 83

Query: 109 PLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRW 168
           P++ +A L  VYL       P+ +                        ++ K G     W
Sbjct: 84  PMMVVACLGCVYLHFKNRKSPHHI-----------------------DRETKGGV----W 143

Query: 169 RLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISC-- 228
                P+LV+GPL +V+  E+  + + +  + W   +Y     AT   ++      S   
Sbjct: 144 SKLRKPMLVKGPLGIVSVTEITFLAMFVALLLWCFITYLRNSFATITPKSAAAHDESLWQ 203

Query: 229 EVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLH 288
             LE   LRLG +G  CL FLFLP++RGS LL  + +  E + +YH+WLGH+ M +FT+H
Sbjct: 204 AKLESAALRLGLIGNICLAFLFLPVARGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVH 263

Query: 289 GLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYT 348
           GL ++I W      I Q++ W   G++NL G I+L AGL+MW T+ PK+R   FE+FFYT
Sbjct: 264 GLCYIIYWASMHE-ISQMIMWDTKGVSNLAGEIALAAGLVMWATTYPKIRRRFFEVFFYT 323

Query: 349 HQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVEL 408
           H LYIVF+LF  LHVG     IA  G +IF++DRFLRF+QSR  V +++A+ LP  T+EL
Sbjct: 324 HYLYIVFMLFFVLHVGISFSFIALPGFYIFLVDRFLRFLQSRENVRLLAARILPSDTMEL 383

Query: 409 IISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRG 468
             SK   L Y+  S +F+ +  +S LQWHPF+++SS     ++L+I+IK  GKW+ KL  
Sbjct: 384 TFSKNSKLVYSPTSIMFVNIPSISKLQWHPFTITSSSKLEPEKLSIVIKKEGKWSTKLH- 443

Query: 469 KILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDIL 528
                  + +SS      + VSVEGPYG  S   L +E L++V GG GI+PF++++ D++
Sbjct: 444 -------QRLSSSDQIDRLAVSVEGPYGPASADFLRHEALVMVCGGSGITPFISVIRDLI 503

Query: 529 HRIRDGKPCLPKKILVVWAIKNSSELPLLSTL---------------------------- 588
                 + C   KI ++ A K SSE+ +L  +                            
Sbjct: 504 -ATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGLETELSSDINIKIEAFITRDNDA 563

Query: 589 -NEGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILVSLMDFYYINP 648
            +E +    K     P     ++S ++G    +W G  + SS +  +I++ ++  YYI P
Sbjct: 564 GDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAILASSFLIFMIIIGIITRYYIYP 623

Query: 649 FN-----IKKWWYKGLLFFLCMVASVVLFGGLVVCLW--------------SLWEEHISS 708
            +     I     K +++ L +  S++      + LW              ++     +S
Sbjct: 624 IDHNTNKIYSLTSKTIIYILVISVSIMATCSAAM-LWNKKKYGKVESKQVQNVDRPSPTS 683

Query: 709 KRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGK 757
             T+    N + ++ E  P+++L Q+          T + +G RPN  +++  V  +   
Sbjct: 684 SPTSSWGYNSLREI-ESTPQESLVQR----------TNLHFGERPNLKKLLLDVEGS--- 723

BLAST of Cp4.1LG01g08770 vs. ExPASy Swiss-Prot
Match: F4I4K7 (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO3 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-83
Identity = 223/716 (31.15%), Postives = 372/716 (51.96%), Query Frame = 0

Query: 49  KWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGAS-GQTL-FGITGAIFLTFSAPLL 108
           K V+KL   VI    +V+  + P+S    ++ +   A  G+++ FG  G   L +  P++
Sbjct: 35  KNVIKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMI 94

Query: 109 AIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLW 168
            +A L ++YL + +     +                   +N+R  +KK +     ++   
Sbjct: 95  LLASLGSIYLHLKKQTRVNQ-------------------FNSRMDRKKID-----KFGAL 154

Query: 169 TLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTF--LQISCEVL 228
             P+LV+  L +VT  E++ +++ +  + W++ +Y      T   ++L      +    L
Sbjct: 155 KRPMLVKAGLGIVTVTEVMFLMMFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARL 214

Query: 229 EVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLA 288
           + + +RLG  G  CLGFLF P++RGS LL  + +  E +T+YH+WLG+L M +FT HGL 
Sbjct: 215 DSIAVRLGLTGNICLGFLFYPVARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLC 274

Query: 289 FVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQL 348
           + I W+   + + Q+LEW   GI++L G I+L+AGLLMW T+ P +R   FE+FFYTH L
Sbjct: 275 YCIYWISTNQ-VSQMLEWDRTGISHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYL 334

Query: 349 YIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIIS 408
           Y+VF+LF   HVG     I+  G +IFM+DRFLRF+QSR  V ++SA+ LPC TVEL  S
Sbjct: 335 YMVFMLFFVFHVGISYALISFPGFYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFS 394

Query: 409 KPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKIL 468
           K   L Y+  S +F+ +  +S LQWHPF+++SS      +L+++IK  GKW+ KL     
Sbjct: 395 KNPMLMYSPTSILFVNIPSISKLQWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLH---- 454

Query: 469 SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRI 528
                 ++S      + VSVEGPYG  S  +L +++L++V+GG GI+PF++I+ D+L+ +
Sbjct: 455 ----HMLASSNQIDHLAVSVEGPYGPASTDYLRHDSLVMVSGGSGITPFISIIRDLLY-V 514

Query: 529 RDGKPCLPKKILVVWAIKNSSELPLLSTL--NEGEIQP---------------------- 588
                    KI ++ A KNSS+L +L+ +  N  EI                        
Sbjct: 515 SSTNAYKTPKITLICAFKNSSDLSMLNLILPNSTEISSFIDIQIKAFVTREKVSTCNMNI 574

Query: 589 SKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILVSLMDFYYINPF--NIKKW 648
            K  S  P      +S ++G    +W    + SS +  +I+++++  Y+I P   + K++
Sbjct: 575 IKTLSFKPYVSDQPISPILGPNSWLWLATILSSSFMIFIIIIAIISRYHIYPIDQSSKEY 634

Query: 649 W--YKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVNDIEKVGEDIPKDTL 708
              Y  L++ L +  SVV    + +          S  +  + NV+ +  +  +   D L
Sbjct: 635 TSAYTSLIYLLAISISVVATSTVAMLC-----NKKSYFKGLYQNVDALSPLMIESSPDQL 694

Query: 709 AQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLICGPSTLQSSVAR 733
                        T I YG RPN ++++  +  +     +GVL+CGP  ++  VA+
Sbjct: 695 ---------LPEFTNIHYGERPNLNKLLVGLKGS----SVGVLVCGPRKMREEVAK 698

BLAST of Cp4.1LG01g08770 vs. NCBI nr
Match: KAG6600696.1 (Ferric reduction oxidase 7, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1393 bits (3605), Expect = 0.0
Identity = 723/775 (93.29%), Postives = 728/775 (93.94%), Query Frame = 0

Query: 3   MAPKHPSFYNHKASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV 62
           MAPKHPS YN KASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV
Sbjct: 1   MAPKHPS-YNPKASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV 60

Query: 63  WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD 122
           WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD
Sbjct: 61  WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD 120

Query: 123 HPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA 182
            PYE                       R+KKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA
Sbjct: 121 QPYE-----------------------RRKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA 180

Query: 183 ELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL 242
           ELVGILVVLGYVFWA+YSYTLR+LATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL
Sbjct: 181 ELVGILVVLGYVFWALYSYTLRLLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL 240

Query: 243 FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW 302
           FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW
Sbjct: 241 FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW 300

Query: 303 KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS 362
           KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS
Sbjct: 301 KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS 360

Query: 363 IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR 422
           IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR
Sbjct: 361 IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR 420

Query: 423 ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTV 482
           ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTV
Sbjct: 421 ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTV 480

Query: 483 SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK 542
           SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK
Sbjct: 481 SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK 540

Query: 543 NSSELPLLSTLN-------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGD 602
           NSSELPLLSTLN                   EGEIQPSKVTSICPLSKGCNMSVLVGTGD
Sbjct: 541 NSSELPLLSTLNVDSICPFFADKLNIDISIYEGEIQPSKVTSICPLSKGCNMSVLVGTGD 600

Query: 603 NVWSGLYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCL 662
           NVWSGLYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCL
Sbjct: 601 NVWSGLYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCL 660

Query: 663 WSLWEEHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEI 722
           WSLWEEHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEI
Sbjct: 661 WSLWEEHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEI 720

Query: 723 VESVAENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           VESVAE WGKVDIGVLICGPSTLQSSVA+AIRSHNMVRRR HHPIFHFHSHSFDL
Sbjct: 721 VESVAETWGKVDIGVLICGPSTLQSSVAKAIRSHNMVRRRNHHPIFHFHSHSFDL 751

BLAST of Cp4.1LG01g08770 vs. NCBI nr
Match: KAG7031332.1 (Ferric reduction oxidase 7, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 708/756 (93.65%), Postives = 712/756 (94.18%), Query Frame = 0

Query: 3   MAPKHPSFYNHKASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV 62
           MAPKHPSFYN KASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV
Sbjct: 1   MAPKHPSFYNPKASMDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSV 60

Query: 63  WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD 122
           WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD
Sbjct: 61  WMVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD 120

Query: 123 HPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA 182
            PYE                       RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA
Sbjct: 121 QPYE-----------------------RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAA 180

Query: 183 ELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL 242
           ELVGILVVLGYVFWA+YSYTLR+LATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL
Sbjct: 181 ELVGILVVLGYVFWALYSYTLRLLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFL 240

Query: 243 FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW 302
           FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW
Sbjct: 241 FLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEW 300

Query: 303 KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS 362
           KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS
Sbjct: 301 KDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFS 360

Query: 363 IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR 422
           IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR
Sbjct: 361 IAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVR 420

Query: 423 ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTV 482
           ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTV
Sbjct: 421 ELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTV 480

Query: 483 SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK 542
           SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK
Sbjct: 481 SVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIK 540

Query: 543 NSSELPLLSTLNEGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILV 602
           NSSELPLLSTLN                 GCNMSVLVGTGDNVWSGLYVISSTVGLVILV
Sbjct: 541 NSSELPLLSTLN-----------------GCNMSVLVGTGDNVWSGLYVISSTVGLVILV 600

Query: 603 SLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVN 662
           SLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVN
Sbjct: 601 SLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVN 660

Query: 663 DIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLICG 722
           DIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAE WGKVDIGVLICG
Sbjct: 661 DIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAETWGKVDIGVLICG 716

Query: 723 PSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           PSTLQSSVA+AIRSHNMVRRR HHPIFHFHSHSFDL
Sbjct: 721 PSTLQSSVAKAIRSHNMVRRRNHHPIFHFHSHSFDL 716

BLAST of Cp4.1LG01g08770 vs. NCBI nr
Match: XP_022976993.1 (ferric reduction oxidase 7, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1364 bits (3531), Expect = 0.0
Identity = 710/770 (92.21%), Postives = 715/770 (92.86%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS
Sbjct: 1   MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
           HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD PYE          
Sbjct: 61  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDQPYE---------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                        R+KKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW
Sbjct: 121 -------------RRKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A YSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI
Sbjct: 181 AFYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTMLIFTLHGLAF+IQWLIQGRCIEQLLEWKDIGIANLPGVISL
Sbjct: 241 DIPFEHATRYHVWLGHLTMLIFTLHGLAFLIQWLIQGRCIEQLLEWKDIGIANLPGVISL 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRFIQSRRTVDVISAKALPCGTVELIISKP+SLRYNALSFIFLQVRELSLLQWHPFSVSS
Sbjct: 361 LRFIQSRRTVDVISAKALPCGTVELIISKPKSLRYNALSFIFLQVRELSLLQWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQPSKVTSICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVGLWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA
Sbjct: 661 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNMVRRRTHHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNMVRRRTHHPIFHFHSHSFDL 747

BLAST of Cp4.1LG01g08770 vs. NCBI nr
Match: XP_022977000.1 (ferric reduction oxidase 7, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1362 bits (3526), Expect = 0.0
Identity = 709/770 (92.08%), Postives = 714/770 (92.73%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS
Sbjct: 1   MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
           HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD PYE          
Sbjct: 61  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDQPYE---------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                         +KKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW
Sbjct: 121 --------------RKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A YSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI
Sbjct: 181 AFYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTMLIFTLHGLAF+IQWLIQGRCIEQLLEWKDIGIANLPGVISL
Sbjct: 241 DIPFEHATRYHVWLGHLTMLIFTLHGLAFLIQWLIQGRCIEQLLEWKDIGIANLPGVISL 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRFIQSRRTVDVISAKALPCGTVELIISKP+SLRYNALSFIFLQVRELSLLQWHPFSVSS
Sbjct: 361 LRFIQSRRTVDVISAKALPCGTVELIISKPKSLRYNALSFIFLQVRELSLLQWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQPSKVTSICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVGLWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA
Sbjct: 661 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNMVRRRTHHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNMVRRRTHHPIFHFHSHSFDL 746

BLAST of Cp4.1LG01g08770 vs. NCBI nr
Match: XP_023542912.1 (ferric reduction oxidase 7, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1336 bits (3457), Expect = 0.0
Identity = 700/764 (91.62%), Postives = 703/764 (92.02%), Query Frame = 0

Query: 25  HPLLPA--PEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW 84
           HP LP   P     ++ +TSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW
Sbjct: 201 HPSLPRSLPSRASSSSSSTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW 260

Query: 85  FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLL 144
           FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYE                
Sbjct: 261 FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYE---------------- 320

Query: 145 NLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT 204
                  RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT
Sbjct: 321 -------RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT 380

Query: 205 LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH 264
           LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH
Sbjct: 381 LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH 440

Query: 265 ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM 324
           ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM
Sbjct: 441 ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM 500

Query: 325 WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS 384
           WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS
Sbjct: 501 WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS 560

Query: 385 RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE 444
           RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE
Sbjct: 561 RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE 620

Query: 445 DRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI 504
           DRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI
Sbjct: 621 DRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI 680

Query: 505 LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-------- 564
           LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN        
Sbjct: 681 LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVDSICPFF 740

Query: 565 --------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS 624
                               EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS
Sbjct: 741 ADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS 800

Query: 625 TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK 684
           TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK
Sbjct: 801 TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK 860

Query: 685 RTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV 744
           RTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV
Sbjct: 861 RTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV 920

Query: 745 DIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           DIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL
Sbjct: 921 DIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 941

BLAST of Cp4.1LG01g08770 vs. ExPASy TrEMBL
Match: A0A6J1INQ8 (ferric reduction oxidase 7, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477191 PE=4 SV=1)

HSP 1 Score: 1364 bits (3531), Expect = 0.0
Identity = 710/770 (92.21%), Postives = 715/770 (92.86%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS
Sbjct: 1   MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
           HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD PYE          
Sbjct: 61  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDQPYE---------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                        R+KKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW
Sbjct: 121 -------------RRKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A YSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI
Sbjct: 181 AFYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTMLIFTLHGLAF+IQWLIQGRCIEQLLEWKDIGIANLPGVISL
Sbjct: 241 DIPFEHATRYHVWLGHLTMLIFTLHGLAFLIQWLIQGRCIEQLLEWKDIGIANLPGVISL 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRFIQSRRTVDVISAKALPCGTVELIISKP+SLRYNALSFIFLQVRELSLLQWHPFSVSS
Sbjct: 361 LRFIQSRRTVDVISAKALPCGTVELIISKPKSLRYNALSFIFLQVRELSLLQWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQPSKVTSICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVGLWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA
Sbjct: 661 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNMVRRRTHHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNMVRRRTHHPIFHFHSHSFDL 747

BLAST of Cp4.1LG01g08770 vs. ExPASy TrEMBL
Match: A0A6J1IL19 (ferric reduction oxidase 7, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477191 PE=4 SV=1)

HSP 1 Score: 1362 bits (3526), Expect = 0.0
Identity = 709/770 (92.08%), Postives = 714/770 (92.73%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS
Sbjct: 1   MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
           HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD PYE          
Sbjct: 61  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDQPYE---------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                         +KKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW
Sbjct: 121 --------------RKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A YSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI
Sbjct: 181 AFYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTMLIFTLHGLAF+IQWLIQGRCIEQLLEWKDIGIANLPGVISL
Sbjct: 241 DIPFEHATRYHVWLGHLTMLIFTLHGLAFLIQWLIQGRCIEQLLEWKDIGIANLPGVISL 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRFIQSRRTVDVISAKALPCGTVELIISKP+SLRYNALSFIFLQVRELSLLQWHPFSVSS
Sbjct: 361 LRFIQSRRTVDVISAKALPCGTVELIISKPKSLRYNALSFIFLQVRELSLLQWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGEDRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGEDRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQPSKVTSICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVGLWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA
Sbjct: 661 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNMVRRRTHHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNMVRRRTHHPIFHFHSHSFDL 746

BLAST of Cp4.1LG01g08770 vs. ExPASy TrEMBL
Match: A0A6J1FSZ3 (ferric reduction oxidase 7, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447866 PE=4 SV=1)

HSP 1 Score: 1326 bits (3433), Expect = 0.0
Identity = 695/764 (90.97%), Postives = 701/764 (91.75%), Query Frame = 0

Query: 25  HPLLPA--PEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW 84
           HP LP   P     ++ +TSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW
Sbjct: 198 HPSLPRSLPSRASSSSSSTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEW 257

Query: 85  FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLL 144
           FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDD PYE                
Sbjct: 258 FGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDQPYE---------------- 317

Query: 145 NLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT 204
                  RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT
Sbjct: 318 -------RKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYT 377

Query: 205 LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH 264
           LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH
Sbjct: 378 LRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEH 437

Query: 265 ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM 324
           ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM
Sbjct: 438 ATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLM 497

Query: 325 WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS 384
           WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS
Sbjct: 498 WITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQS 557

Query: 385 RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE 444
           RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE
Sbjct: 558 RRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGE 617

Query: 445 DRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI 504
           DRLAILIKVLGKWTEKLRGKIL+DKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI
Sbjct: 618 DRLAILIKVLGKWTEKLRGKILNDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLI 677

Query: 505 LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-------- 564
           LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN        
Sbjct: 678 LVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNVDSICPFF 737

Query: 565 --------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS 624
                               EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS
Sbjct: 738 ADKLNIDISIYVTRQSQPPPEGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISS 797

Query: 625 TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK 684
           TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK
Sbjct: 798 TVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSK 857

Query: 685 RTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV 744
           RTTH+NVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV
Sbjct: 858 RTTHHNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKV 917

Query: 745 DIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           DIGVLICGPSTLQSSVA+AIRSHNMVRRR HHPIFHFHSHSFDL
Sbjct: 918 DIGVLICGPSTLQSSVAKAIRSHNMVRRRAHHPIFHFHSHSFDL 938

BLAST of Cp4.1LG01g08770 vs. ExPASy TrEMBL
Match: A0A1S3BSM7 (ferric reduction oxidase 7, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493281 PE=4 SV=1)

HSP 1 Score: 1153 bits (2982), Expect = 0.0
Identity = 600/770 (77.92%), Postives = 654/770 (84.94%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDNEHSPHHPLLPA  +Q+PATG +SAIVS  KW+LK AMWVIF VW++VIFLYPSS  +
Sbjct: 1   MDNEHSPHHPLLPATHEQYPATGKSSAIVSIAKWILKSAMWVIFLVWILVIFLYPSSLTT 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
            LF+ W  A+ Q+LFG+TGAIFLTFSAPLLAIAI+AAVYLIISRDD PY           
Sbjct: 61  QLFNNWVEATQQSLFGLTGAIFLTFSAPLLAIAIIAAVYLIISRDDEPY----------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                            KK  KEKPRWRLWTLPVLVEGP EVVTAAE VGILVVLGYVFW
Sbjct: 121 -----------------KKKIKEKPRWRLWTLPVLVEGPFEVVTAAEFVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A+Y YTLRMLA D+ R+ TF ++S  VLEVMG+RLGS+GLFCLGFLFLPISRGS+LLRLI
Sbjct: 181 ALYCYTLRMLAADYFRHSTFKELSAVVLEVMGVRLGSIGLFCLGFLFLPISRGSILLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTM+IFTLH LAFVI W I+GR ++QL+EWKD+GIANLPGVIS+
Sbjct: 241 DIPFEHATRYHVWLGHLTMIIFTLHSLAFVIVWSIEGRLLQQLMEWKDVGIANLPGVISI 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRT NFELFFYTHQLYI+FV+FLALHVGDFVF+IAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTKNFELFFYTHQLYIIFVVFLALHVGDFVFTIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRFIQSR TVDVISAKALPCGTVEL++SKP+SLRYNALSFIFLQVRELSLL+WHPFSVSS
Sbjct: 361 LRFIQSRTTVDVISAKALPCGTVELVLSKPKSLRYNALSFIFLQVRELSLLEWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGEDRLAILIKVLGKWTE+LRGKIL+DKAK+ISS+KH  VMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGEDRLAILIKVLGKWTERLRGKILNDKAKQISSEKHSPVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSD+LHRIRDGK CLPKKILVVWAIK S EL LLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDVLHRIRDGKACLPKKILVVWAIKTSDELSLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQ SKVTSICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADILNIDISIYVTRQSQPPSEGEIQGSKVTSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVI ST+GLVILV LMDF+YINPF+I KWWYKGLLF LCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVIFSTLGLVILVGLMDFFYINPFHIIKWWYKGLLFLLCMVASVVLFGGLVVALWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           ++ISSKRT+ +N NDIE V ++ PK + AQKDLN  A A STTIEYG RPNF+EI+ SV+
Sbjct: 661 QYISSKRTSDHN-NDIENVDQESPKHSFAQKDLNSNALATSTTIEYGLRPNFEEILGSVS 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNM  RR+HHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNM-GRRSHHPIFHFHSHSFDL 740

BLAST of Cp4.1LG01g08770 vs. ExPASy TrEMBL
Match: A0A0A0L3F3 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G113400 PE=4 SV=1)

HSP 1 Score: 1135 bits (2937), Expect = 0.0
Identity = 593/770 (77.01%), Postives = 645/770 (83.77%), Query Frame = 0

Query: 17  MDNEHSPHHPLLPAPEDQFPATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFAS 76
           MDN+HSPHHPLLP   +Q+P T   SAIVS  KWVLKLAMWVIF VWM VIFLYPSS A+
Sbjct: 1   MDNQHSPHHPLLPPTHEQYPDTAKPSAIVSLAKWVLKLAMWVIFLVWMSVIFLYPSSLAT 60

Query: 77  HLFSEWFGASGQTLFGITGAIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLF 136
            LF  W  A+ Q+ FGITGAIFLTFSAPLLAIAI+AAVYLI+S D+ PY           
Sbjct: 61  QLFENWVQATQQSPFGITGAIFLTFSAPLLAIAIIAAVYLIVSHDEEPY----------- 120

Query: 137 FLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFW 196
                            KK  KEKPRWRLWT+PVLVEGPLEVVTA ELVGILVVLGYVFW
Sbjct: 121 -----------------KKKIKEKPRWRLWTMPVLVEGPLEVVTAGELVGILVVLGYVFW 180

Query: 197 AIYSYTLRMLATDFSRNLTFLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLI 256
           A+Y YTLRMLA D+  + TF ++S  VLEV+G+RLGS+GLFCLGFLFLPISRGS+LLRLI
Sbjct: 181 ALYCYTLRMLAADYFTHSTFKELSVVVLEVIGVRLGSIGLFCLGFLFLPISRGSILLRLI 240

Query: 257 DIPFEHATRYHVWLGHLTMLIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISL 316
           DIPFEHATRYHVWLGHLTM+IFTLH LAFVI W IQGR ++QL+EWKD+GIANLPGVIS+
Sbjct: 241 DIPFEHATRYHVWLGHLTMIIFTLHSLAFVIGWSIQGRLLQQLMEWKDVGIANLPGVISI 300

Query: 317 LAGLLMWITSLPKLRTINFELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRF 376
           LAGLLMWITSLPKLRT NFELFFYTHQLYI+FV+FLALHVGDFVFSIAAGGIFIFMLDRF
Sbjct: 301 LAGLLMWITSLPKLRTKNFELFFYTHQLYIIFVVFLALHVGDFVFSIAAGGIFIFMLDRF 360

Query: 377 LRFIQSRRTVDVISAKALPCGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSS 436
           LRF+QSR TVDVISAKALPCGTVEL++SKP+SLRYNALSFIFLQVRELSLL+WHPFSVSS
Sbjct: 361 LRFVQSRTTVDVISAKALPCGTVELVLSKPKSLRYNALSFIFLQVRELSLLEWHPFSVSS 420

Query: 437 SPLEGEDRLAILIKVLGKWTEKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHL 496
           SPLEGE+RLAILIKVLGKWTE+LRGKIL+DKAK+ISSDKH  VMTVSVEGPYGHESPYHL
Sbjct: 421 SPLEGENRLAILIKVLGKWTERLRGKILNDKAKQISSDKHSPVMTVSVEGPYGHESPYHL 480

Query: 497 MYENLILVAGGIGISPFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN-- 556
           MYENLILVAGGIGISPFLAILSD+LHRIRDGK CLPKKILVVWAIK S ELPLLSTLN  
Sbjct: 481 MYENLILVAGGIGISPFLAILSDVLHRIRDGKTCLPKKILVVWAIKTSDELPLLSTLNVD 540

Query: 557 --------------------------EGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSG 616
                                     EGEIQ SKV+SICPLSKG NMSVLVGTGDNVWSG
Sbjct: 541 SICPFFADKLNIDISIYVTRQSQPPSEGEIQGSKVSSICPLSKGSNMSVLVGTGDNVWSG 600

Query: 617 LYVISSTVGLVILVSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWE 676
           LYVI ST+GLV LV  MD +YINPF+I KWWYKGLLF LCMVASVVLFGGLVV LWSLWE
Sbjct: 601 LYVIFSTLGLVFLVGFMDLFYINPFHIIKWWYKGLLFLLCMVASVVLFGGLVVALWSLWE 660

Query: 677 EHISSKRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVA 736
           ++ISSK T+ +N NDIE V E+ PK + AQKDLN  A A STTIEYG RPNF+EI+ SV+
Sbjct: 661 QYISSKGTSDHN-NDIENVDEESPKHSFAQKDLNSNALATSTTIEYGLRPNFEEILGSVS 720

Query: 737 ENWGKVDIGVLICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 758
           ENWGKVDIGVLICGPSTLQSSVA+AIRSHNM  RR+HHPIFHFHSHSFDL
Sbjct: 721 ENWGKVDIGVLICGPSTLQSSVAKAIRSHNM-GRRSHHPIFHFHSHSFDL 740

BLAST of Cp4.1LG01g08770 vs. TAIR 10
Match: AT5G49740.1 (ferric reduction oxidase 7 )

HSP 1 Score: 778.5 bits (2009), Expect = 5.0e-225
Identity = 418/758 (55.15%), Postives = 528/758 (69.66%), Query Frame = 0

Query: 37  ATGNTSAIVSSTKWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGASGQTLFGITGA 96
           ++ ++S +VSS KW+LK+ M VIF  W+V + +YP S    + + W   S  TLFG+TG+
Sbjct: 20  SSSSSSVVVSSLKWILKVVMSVIFVTWVVFLMMYPGSLGDQILTNWRAISSNTLFGLTGS 79

Query: 97  IFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKN 156
           +FL FS P+L IAILA++YLIIS ++  +                            KK 
Sbjct: 80  MFLIFSGPILVIAILASLYLIISGEETVF---------------------------TKKK 139

Query: 157 GKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTF 216
             + PR+RLWT PVLV+GP  VV+AAE +GI+V   +  WAIY+YTLR L      ++  
Sbjct: 140 ITKFPRFRLWTFPVLVDGPFGVVSAAEFLGIMVFSVFFLWAIYAYTLRNLNVLDYFHVLP 199

Query: 217 LQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTML 276
              S  +LE+ GLR G +GL C+ FLFLPISRGS+LLRLIDIPFEHATRYHVWLGH+TM 
Sbjct: 200 NNRSIFLLELTGLRFGMIGLLCMVFLFLPISRGSILLRLIDIPFEHATRYHVWLGHITMT 259

Query: 277 IFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFE 336
            F+LHGL +V+ W IQG+ +E L EWK  GIA LPGVISL+AGLLMW+TSL  +R   FE
Sbjct: 260 FFSLHGLCYVVGWTIQGQLLELLFEWKATGIAVLPGVISLVAGLLMWVTSLHTVRKNYFE 319

Query: 337 LFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPC 396
           LFFYTHQLYIVFV+FLALHVGD++FSI AGGIF+F+LDRFLRF QSRRTVDVISAK+LPC
Sbjct: 320 LFFYTHQLYIVFVVFLALHVGDYLFSIVAGGIFLFILDRFLRFYQSRRTVDVISAKSLPC 379

Query: 397 GTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWT 456
           GT+EL++SKP ++RYNALSFIFLQV+ELS LQWHPFSVSSSPL+G   +A+LIKVLG WT
Sbjct: 380 GTLELVLSKPPNMRYNALSFIFLQVKELSWLQWHPFSVSSSPLDGNHHVAVLIKVLGGWT 439

Query: 457 EKLRGKIL----SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISP 516
            KLR ++     ++   ++ S +    +T  VEGPYGHESPYHL YENL+LVAGGIGI+P
Sbjct: 440 AKLRDQLSTLYEAENQDQLISPESYPKITTCVEGPYGHESPYHLAYENLVLVAGGIGITP 499

Query: 517 FLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLN--------------EGEI 576
           F AILSDILHR RDGK CLP K+LVVWAIKNS EL LLS ++              E  I
Sbjct: 500 FFAILSDILHRKRDGKDCLPGKVLVVWAIKNSDELSLLSAIDIPSICHFFSKKLNLEIHI 559

Query: 577 QPSKVTSIC----------------PLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILV 636
             ++ +  C                P + GC+MSVLVGTGDN+WSGLY+I ST+G + ++
Sbjct: 560 YVTRQSEPCLEDGMVHKVVHPSVKTPWTNGCSMSVLVGTGDNIWSGLYLIISTIGFIAMI 619

Query: 637 SLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVN 696
           +L+D +YIN +NI  WWYKGLLF +CMVASV++FGGLVV  W  WE          N+  
Sbjct: 620 TLVDIFYINKYNITTWWYKGLLFVVCMVASVLIFGGLVVVFWHRWEHKTGEVEANGNDKV 679

Query: 697 DIEKVGEDIPKDTLAQ-KDLN-PKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLI 756
           D+   GE+    + A+ K L   +   N TTI YGTRP F EI ES+   WG VD+GV++
Sbjct: 680 DLN--GEETHNPSAAELKGLAIEEDVQNYTTIRYGTRPAFREIFESLNGKWGSVDVGVIV 739

Query: 757 CGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 759
           CGP+TLQ++VA+ IRSH+ + R  +HP+FHF+SHSFDL
Sbjct: 740 CGPATLQTTVAKEIRSHS-IWRSANHPLFHFNSHSFDL 747

BLAST of Cp4.1LG01g08770 vs. TAIR 10
Match: AT5G49730.1 (ferric reduction oxidase 6 )

HSP 1 Score: 753.8 bits (1945), Expect = 1.3e-217
Identity = 413/759 (54.41%), Postives = 524/759 (69.04%), Query Frame = 0

Query: 37  ATGNTSAIVSSTKWVLKLAMWVIFSVWMV-VIFLYPSSFASHLFSEWFGASGQTLFGITG 96
           ++ ++S I SS KW+LK+ M +IF  W+V ++ +YP        +     S +T  G TG
Sbjct: 13  SSSSSSVITSSLKWILKVVMSMIFVTWVVFLMMMYPEQLGDDFLT---FVSSKTFLGTTG 72

Query: 97  AIFLTFSAPLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKK 156
            +FL FS P+L I++LA++YLIIS +D   ++   K+IS F                   
Sbjct: 73  TMFLIFSCPILVISVLASLYLIISGED---KVFTKKKISKF------------------- 132

Query: 157 NGKEKPRWRLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLT 216
                PR+RLWT PVLV+GP  VV+AAE +GI+V   +  W+IY+YTLR L      ++ 
Sbjct: 133 -----PRFRLWTFPVLVDGPFGVVSAAEFLGIMVFSVFFLWSIYAYTLRNLDLLERFHVL 192

Query: 217 FLQISCEVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTM 276
               S  +LEV GLRLG +GL C+ FLFLPISRGS+LLRLIDIPFEHATRYHVWLGH+TM
Sbjct: 193 PKNRSILLLEVTGLRLGMIGLLCMVFLFLPISRGSILLRLIDIPFEHATRYHVWLGHITM 252

Query: 277 LIFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINF 336
             F+LHGL +V+ W+IQG+ +E +  W  IGIA LPGVISL+AGLLMW+TSL  +R   F
Sbjct: 253 AFFSLHGLCYVVGWIIQGQLLELIFSWNAIGIAILPGVISLVAGLLMWVTSLHTVRKNYF 312

Query: 337 ELFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALP 396
           ELFFYTHQLYIVF++FLALHVGD++FSI AGGIF+F+LDRFLRF QSRRTVDVISAK+LP
Sbjct: 313 ELFFYTHQLYIVFIVFLALHVGDYMFSIVAGGIFLFILDRFLRFCQSRRTVDVISAKSLP 372

Query: 397 CGTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKW 456
           CGT+EL++SKP ++RYNALSFIFLQVRELS LQWHPFSVSSSPL+G   +A+LIKVLG W
Sbjct: 373 CGTLELVLSKPPNMRYNALSFIFLQVRELSWLQWHPFSVSSSPLDGNHHVAVLIKVLGGW 432

Query: 457 TEKLRGKIL----SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGIS 516
           T KLR ++     ++   ++ S +    +T  VEGPYGHESPYHL YENL+LVAGGIGI+
Sbjct: 433 TAKLRDQLSNLYEAENQDQLISPQSYPKITTCVEGPYGHESPYHLAYENLVLVAGGIGIT 492

Query: 517 PFLAILSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTLNEGEIQP--SKVTSI--- 576
           PF AILSDILHR RDGK CLP K+LVVWAIKNS EL LLS ++   I P  SK  ++   
Sbjct: 493 PFFAILSDILHRKRDGKACLPSKVLVVWAIKNSDELSLLSAIDIPSICPFFSKKLNLEIH 552

Query: 577 -------------------------CPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVIL 636
                                     P + GC+MSVLVGTGDN+WSGLY+I ST+G + +
Sbjct: 553 IYITRQSEPRLEDGMVHKVVHPSVKLPRTNGCSMSVLVGTGDNIWSGLYLIISTIGFISM 612

Query: 637 VSLMDFYYINPFNIKKWWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNV 696
           ++L+D +YI  +NI  WWYKGLLF  CMVASV++FGGLVV  W  WE          N+ 
Sbjct: 613 ITLLDIFYIKRYNITTWWYKGLLFVGCMVASVLIFGGLVVVFWHRWEHKTGEVEANGNDK 672

Query: 697 NDIEKVGEDIPKDTLAQ-KDLN-PKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVL 756
            D+   GE+    + A+ K L   +   N TTI YGTRP F EI ES+   WG VD+GV+
Sbjct: 673 VDLN--GEETHNPSAAELKGLAIEEDVQNYTTIRYGTRPAFREIFESLNGKWGSVDVGVI 732

Query: 757 ICGPSTLQSSVARAIRSHNMVRRRTHHPIFHFHSHSFDL 759
           +CGP+TLQ++VA+ IRSH+ + R  +HP+FHF+SHSFDL
Sbjct: 733 VCGPATLQTTVAKEIRSHS-IWRSANHPLFHFNSHSFDL 738

BLAST of Cp4.1LG01g08770 vs. TAIR 10
Match: AT1G01590.1 (ferric reduction oxidase 1 )

HSP 1 Score: 321.2 bits (822), Expect = 2.2e-87
Identity = 225/732 (30.74%), Postives = 381/732 (52.05%), Query Frame = 0

Query: 51  VLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGAS-GQTL-FGITGAIFLTFSAPLLAI 110
           V+K  M VI    +V+  + P+S    ++     A  G+++ +G  G   L +  P++ +
Sbjct: 14  VIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILL 73

Query: 111 AILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLWTL 170
           A L  +YL + +                     +N   +   KK+ K G  +        
Sbjct: 74  AFLGCIYLHLKKST------------------TVNQFNSGVEKKRAKFGALR-------R 133

Query: 171 PVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISCEV----- 230
           P+LV GPL +VT  E++ + + +  + W++ +Y  R          TF+ ++ E      
Sbjct: 134 PMLVNGPLGIVTVTEVMFLTMFMALLLWSLANYMYR----------TFVNVTSESAATDG 193

Query: 231 -------LEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTML 290
                  L+++ +R+G +G  CL FLF P++RGS LL  + +  E + +YH+WLGHL M+
Sbjct: 194 NNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSLLAAVGLTSESSIKYHIWLGHLVMI 253

Query: 291 IFTLHGLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFE 350
           IFT HGL + I W+ + + + ++LEW    ++NL G I+L+AGL+MW+T+ PK+R   FE
Sbjct: 254 IFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNLAGEIALVAGLMMWVTTYPKIRRRLFE 313

Query: 351 LFFYTHQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPC 410
           +FFY+H LYIVF+LF   HVG     I   G +IF++DRFLRF+QSR  V ++SA+ LPC
Sbjct: 314 VFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYIFLVDRFLRFLQSRNNVKLVSARVLPC 373

Query: 411 GTVELIISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWT 470
            TVEL  SK   L Y+  S +F+ +  +S LQWHPF++ SS     + L+++IK  GKW+
Sbjct: 374 DTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWHPFTIISSSKLEPETLSVMIKSQGKWS 433

Query: 471 EKLRGKILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAI 530
            KL   + S  + +I+       + VSVEGPYG  S   L +E+L++V+GG GI+PF++I
Sbjct: 434 TKLYDMLSSSSSDQINR------LAVSVEGPYGPSSTDFLRHESLVMVSGGSGITPFISI 493

Query: 531 LSDILHRIRDGKPCLPKKILVVWAIKNSSELPLLSTL-----------------NEGEIQ 590
           + D+ +     K  +PK  L+  A KNSS+L +L  +                  +  + 
Sbjct: 494 VRDLFYMSSTHKCKIPKMTLIC-AFKNSSDLSMLDLILPTSGLTTDMASFVDIQIKAFVT 553

Query: 591 PSKVTSICPLSKGCNM-------------SVLVGTGDNVWSGLYVI--SSTVGLVILVSL 650
             + TS+   +   N+              +    G N W  L  I  SS +  ++++++
Sbjct: 554 REEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSWLCLAAILSSSFMIFIVIIAI 613

Query: 651 MDFYYINPF--NIKK--WWYKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNN 710
           +  Y+I+P   N +K  W YK L+ +L  ++  V+       LW+  +++ +      +N
Sbjct: 614 ITRYHIHPIDQNSEKYTWAYKSLI-YLVSISITVVTTSTAAMLWNK-KKYYAKNDQYVDN 673

Query: 711 VNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLI 733
           ++ +  + E  P+  ++Q          ST I YG RPN ++++  +  +     +G+L+
Sbjct: 674 LSPV--IIESSPQQLISQ----------STDIHYGERPNLNKLLVGLKGS----SVGILV 685

BLAST of Cp4.1LG01g08770 vs. TAIR 10
Match: AT1G01580.1 (ferric reduction oxidase 2 )

HSP 1 Score: 312.4 bits (799), Expect = 1.0e-84
Identity = 237/763 (31.06%), Postives = 370/763 (48.49%), Query Frame = 0

Query: 49  KWVLKLAMWVIF----SVW-MVVIFLYPSSFASHLFSEWFGASGQTLFGITGAIFLTFSA 108
           K V K  M VIF     +W M+    Y + +  HL  + FG S  T FG TG     +  
Sbjct: 24  KGVTKFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIK-FGTS--TYFGATGTTLFMYMF 83

Query: 109 PLLAIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRW 168
           P++ +A L  VYL       P+ +                        ++ K G     W
Sbjct: 84  PMMVVACLGCVYLHFKNRKSPHHI-----------------------DRETKGGV----W 143

Query: 169 RLWTLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTFLQISC-- 228
                P+LV+GPL +V+  E+  + + +  + W   +Y     AT   ++      S   
Sbjct: 144 SKLRKPMLVKGPLGIVSVTEITFLAMFVALLLWCFITYLRNSFATITPKSAAAHDESLWQ 203

Query: 229 EVLEVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLH 288
             LE   LRLG +G  CL FLFLP++RGS LL  + +  E + +YH+WLGH+ M +FT+H
Sbjct: 204 AKLESAALRLGLIGNICLAFLFLPVARGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVH 263

Query: 289 GLAFVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYT 348
           GL ++I W      I Q++ W   G++NL G I+L AGL+MW T+ PK+R   FE+FFYT
Sbjct: 264 GLCYIIYWASMHE-ISQMIMWDTKGVSNLAGEIALAAGLVMWATTYPKIRRRFFEVFFYT 323

Query: 349 HQLYIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVEL 408
           H LYIVF+LF  LHVG     IA  G +IF++DRFLRF+QSR  V +++A+ LP  T+EL
Sbjct: 324 HYLYIVFMLFFVLHVGISFSFIALPGFYIFLVDRFLRFLQSRENVRLLAARILPSDTMEL 383

Query: 409 IISKPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRG 468
             SK   L Y+  S +F+ +  +S LQWHPF+++SS     ++L+I+IK  GKW+ KL  
Sbjct: 384 TFSKNSKLVYSPTSIMFVNIPSISKLQWHPFTITSSSKLEPEKLSIVIKKEGKWSTKLH- 443

Query: 469 KILSDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDIL 528
                  + +SS      + VSVEGPYG  S   L +E L++V GG GI+PF++++ D++
Sbjct: 444 -------QRLSSSDQIDRLAVSVEGPYGPASADFLRHEALVMVCGGSGITPFISVIRDLI 503

Query: 529 HRIRDGKPCLPKKILVVWAIKNSSELPLLSTL---------------------------- 588
                 + C   KI ++ A K SSE+ +L  +                            
Sbjct: 504 -ATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGLETELSSDINIKIEAFITRDNDA 563

Query: 589 -NEGEIQPSKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILVSLMDFYYINP 648
            +E +    K     P     ++S ++G    +W G  + SS +  +I++ ++  YYI P
Sbjct: 564 GDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAILASSFLIFMIIIGIITRYYIYP 623

Query: 649 FN-----IKKWWYKGLLFFLCMVASVVLFGGLVVCLW--------------SLWEEHISS 708
            +     I     K +++ L +  S++      + LW              ++     +S
Sbjct: 624 IDHNTNKIYSLTSKTIIYILVISVSIMATCSAAM-LWNKKKYGKVESKQVQNVDRPSPTS 683

Query: 709 KRTTHNNVNDIEKVGEDIPKDTLAQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGK 757
             T+    N + ++ E  P+++L Q+          T + +G RPN  +++  V  +   
Sbjct: 684 SPTSSWGYNSLREI-ESTPQESLVQR----------TNLHFGERPNLKKLLLDVEGS--- 723

BLAST of Cp4.1LG01g08770 vs. TAIR 10
Match: AT1G23020.2 (ferric reduction oxidase 3 )

HSP 1 Score: 312.4 bits (799), Expect = 1.0e-84
Identity = 223/716 (31.15%), Postives = 372/716 (51.96%), Query Frame = 0

Query: 49  KWVLKLAMWVIFSVWMVVIFLYPSSFASHLFSEWFGAS-GQTL-FGITGAIFLTFSAPLL 108
           K V+KL   VI    +V+  + P+S    ++ +   A  G+++ FG  G   L +  P++
Sbjct: 35  KNVIKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMI 94

Query: 109 AIAILAAVYLIISRDDHPYELVIFKQISLFFLVFLLNLKYNNRRKKKKKNGKEKPRWRLW 168
            +A L ++YL + +     +                   +N+R  +KK +     ++   
Sbjct: 95  LLASLGSIYLHLKKQTRVNQ-------------------FNSRMDRKKID-----KFGAL 154

Query: 169 TLPVLVEGPLEVVTAAELVGILVVLGYVFWAIYSYTLRMLATDFSRNLTF--LQISCEVL 228
             P+LV+  L +VT  E++ +++ +  + W++ +Y      T   ++L      +    L
Sbjct: 155 KRPMLVKAGLGIVTVTEVMFLMMFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARL 214

Query: 229 EVMGLRLGSMGLFCLGFLFLPISRGSVLLRLIDIPFEHATRYHVWLGHLTMLIFTLHGLA 288
           + + +RLG  G  CLGFLF P++RGS LL  + +  E +T+YH+WLG+L M +FT HGL 
Sbjct: 215 DSIAVRLGLTGNICLGFLFYPVARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLC 274

Query: 289 FVIQWLIQGRCIEQLLEWKDIGIANLPGVISLLAGLLMWITSLPKLRTINFELFFYTHQL 348
           + I W+   + + Q+LEW   GI++L G I+L+AGLLMW T+ P +R   FE+FFYTH L
Sbjct: 275 YCIYWISTNQ-VSQMLEWDRTGISHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYL 334

Query: 349 YIVFVLFLALHVGDFVFSIAAGGIFIFMLDRFLRFIQSRRTVDVISAKALPCGTVELIIS 408
           Y+VF+LF   HVG     I+  G +IFM+DRFLRF+QSR  V ++SA+ LPC TVEL  S
Sbjct: 335 YMVFMLFFVFHVGISYALISFPGFYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFS 394

Query: 409 KPRSLRYNALSFIFLQVRELSLLQWHPFSVSSSPLEGEDRLAILIKVLGKWTEKLRGKIL 468
           K   L Y+  S +F+ +  +S LQWHPF+++SS      +L+++IK  GKW+ KL     
Sbjct: 395 KNPMLMYSPTSILFVNIPSISKLQWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLH---- 454

Query: 469 SDKAKEISSDKHQSVMTVSVEGPYGHESPYHLMYENLILVAGGIGISPFLAILSDILHRI 528
                 ++S      + VSVEGPYG  S  +L +++L++V+GG GI+PF++I+ D+L+ +
Sbjct: 455 ----HMLASSNQIDHLAVSVEGPYGPASTDYLRHDSLVMVSGGSGITPFISIIRDLLY-V 514

Query: 529 RDGKPCLPKKILVVWAIKNSSELPLLSTL--NEGEIQP---------------------- 588
                    KI ++ A KNSS+L +L+ +  N  EI                        
Sbjct: 515 SSTNAYKTPKITLICAFKNSSDLSMLNLILPNSTEISSFIDIQIKAFVTREKVSTCNMNI 574

Query: 589 SKVTSICPLSKGCNMSVLVGTGDNVWSGLYVISSTVGLVILVSLMDFYYINPF--NIKKW 648
            K  S  P      +S ++G    +W    + SS +  +I+++++  Y+I P   + K++
Sbjct: 575 IKTLSFKPYVSDQPISPILGPNSWLWLATILSSSFMIFIIIIAIISRYHIYPIDQSSKEY 634

Query: 649 W--YKGLLFFLCMVASVVLFGGLVVCLWSLWEEHISSKRTTHNNVNDIEKVGEDIPKDTL 708
              Y  L++ L +  SVV    + +          S  +  + NV+ +  +  +   D L
Sbjct: 635 TSAYTSLIYLLAISISVVATSTVAMLC-----NKKSYFKGLYQNVDALSPLMIESSPDQL 694

Query: 709 AQKDLNPKAFANSTTIEYGTRPNFDEIVESVAENWGKVDIGVLICGPSTLQSSVAR 733
                        T I YG RPN ++++  +  +     +GVL+CGP  ++  VA+
Sbjct: 695 ---------LPEFTNIHYGERPNLNKLLVGLKGS----SVGVLVCGPRKMREEVAK 698