Cp4.1LG01g08540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g08540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionelongation factor 1-gamma-like
LocationCp4.1LG01: 4837950 .. 4841307 (+)
RNA-Seq ExpressionCp4.1LG01g08540
SyntenyCp4.1LG01g08540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAACAAAATTAGGCTGGGTGTGTCGGGCCATGGAGCCCAAAGACGAATACCCGATCCTGAAACACCTTTAAAACCCTAGTTTTTGCTCATTATAACACACCTCCCGCAGCAGGCTTACTCTTGCTATCGCTCGCCCCCAGGTCTCTCTCTCAACTTCATCACTTGTTTCTTTAGTTGATCTTGTCTATTTCATCAGAAATCAATCACAAATCGATTGAATTGTATCTGTATTTCTTTCTGTAACTAACCGCTACGTTTGTTGTTTGCTCAGATCTGTATTCTTTGAGCGAAACGTTTTTTCTACAGCGAAGCGATGGCTCTGGTGAGTCTCTCGTTACTTCTGATATTTTATACGAATTGGTTCCAGAACGTGGGAATTTATCTTGGATTATTTTGGATCTGACGTGTTGCCGTTTGGTTTGATTGATGGGTTTTATGGAAAAATGGAAACTGTAATTGGATAGTTCTAGCGTAGGGTTCATGTTTTATTCCGCTTTTCAACTCTGGTTTGAATTTTCTGATCTTATTTTTGGACCATGCTATTTTGATAGTAATACTCTTTTGTGTCCGAATCACTACCGTGCGCTGTTTACTCCTGAAATTGGATAGTGTCTCATGTTGACATATGTCATTGCATAAAAAGTGTTGAACGATTGCTGAACTAGTTGCTTTTTTTACATTATTGACGATTGAGGATTGCTTTTCCGTTTTTTTTTTTGCAAGTTCAATCTTATTGTTCTTGGGGTTTCTATTGATTGAGATTTCTTAAAATATTCCAAACTTAGTACTCCCACCCGTTCAAAGAAGTTTATCATGCCAAAGCCTAACGCGTTATTGTCTAATTACACACCAATGAAGTTTATTGTGTGCTTACTCCTTGGTTTCAGTTATTAATGAATTCATTGAAATTGGATTTTGTCATTTTTCAGTTGATAATCGTTTGACATGTTATTGATGTATATACGTGTTCATTTTGCAGGTTCTGTACTCCTGGAACACTAACAAAAATGCTTACAAAGCTCTTATAGTTGCAGAATACAATGGTGTCAAGGTTGACCTTGCCCCAGGTTTTACCATGGGCGTAACCAATAAGTCTCCTGAGTTTCTCAAAATGAATCCTATTGGGAAGGTAAACAAATATCTTGCTCTTGGTCTGGTGATCTGTTCGTGTTAGAGATTGTAGTTCTTTATTCTAGCTCTTTGCTTAAAAAATAATGGATCTTAATGTTTCAGGTTCCTGTGCTTGAAACACCCGAAGGTGGTATCTTTGAGAGCAATGCCATAGCTCGCTATGGTAATTATTGTTATGCTTTCTCCATGGGAGTGACTTAATAACTATGCACACGAGTTAAGCGGAATTATTCCTCTTCTTTTGTTTTATGGTTTTTTTTTTCTTGTTGCTACAGTTGCTCGCTTGAAGGACTGTGGTTTATTTGGTTCTTCTCCAATTGAATATGTAAGATCCTTTGTTCTTATTCTTTGTTGGTTTTGATTATTCTCCTTTTCTTTTCTTTAATTAGAATGAAGTTGTTTGCTTTTATTAAACCTTTTCACTCTATTTGTTTTCAGGGACATGTTGAACAATGGATTGACTTTGCCACACTTGAAATTGATGTTCATATTGGTGCTTTAGCAGGGCCTAGATATGGATATCGTGCTTACCATGCTGCTGTAAGTTAGTTTCTCTTTTATATCTAATCGAGTGAGTTTTTCTTACAATGTGGCTGAATTATTGATAACGTTTTCTGTGCGAATTCTTATTCAAATTTGAATTTTTTCTTCTTGTATTCTTCACTTGTGTCGTGGGTAGTAATAGAAAATGAAAAGTGGATTGAGAAATTGGTCTTTTTGGTTTTCAATTTCACTCTTTTCCATTTTTTTCTTTCCTTCCTGAGTCCTATATTTCATGTATGAGTGATTTTTTTTTTGGTTACCTACGAATGACTTTGCCTTCTTATTTCTCTGGCACATGACACCGTGCATTTTATTTTCATCATAAAGGTTGAAGAAGCTGCAAACGCTGCCCTAAAACGCTCTTTTGCTGCTCTGAACTCTTACCTTGCTTCAAACACATACCTTGTTGGACATTCTGTGACACTGGCTGATATCATTGTAACATGCAATTTGTATTGGGGAATCTCTTTTCTGTTGACTAAAAGCTTTACTTCTGAGTTCCCTCACGTCGAGAGGTACTTCTGGACAATGGTCAATCAACCAAATTTCAAGAAGATTATTGGTGAGTTTAAGCAGACAGAAACTGTTCCACCAGTGAAAACACCGGGAGAGGCTGCAGCAGCTGCCAAGCCAAAGGCTGAGCCTAAGAAGGAAGAGAAGCCCAAAGCAGCAGCACCTGCAGAGGAAGAGGATGCACCAAAGCCCAAGGCCAAAAATCCTCTTGACCTCCTTCCTCCCAGTAAGATGATTTTGGATGAGTGGAAACGTCTCTACTCGAACACAAAAACTAACTTCCGTGAGGTTGCAATCAAAGGTATGGCCATTCTTAAGCCCCATCTGTTTCATTATGTTTCTTGAGAAGTTAAGAATCAATAACTTTTTTCCCCTTGAAGGAATTGTATAATTGAAGTCTTCTATGTTCATTTGTCTACACGTGATGCAGGATTCTGGGACATGTACGATCCTGAAGGGTACTCCCTTTGGTTCTGTGATTACAAGTACAATGACGAGAACACAGTTTCCTTTGTGACTTTGAACAAAGTCAGCGGATTTCTCCAGAGAATGGATCTTGCTCGTAAATATGCCTTTGGAAAGATGCTTGTGATCGGATCAGAGGCACCCTTCAAGGTGAAGGGGCTATGGCTTTTCCGTGGGCAAGAAATCCCACAATTTGTTATGGACGAGTGCTATGACATGGAGCTGTACGAGTGGAAGAAGGTCGATATCTCCGATGAAGCCCAGAAGGAGAGAGTTAACCAAATGATTGAAGATCAGGAACCATTTGAGGGAGAGGCTCTCTTGGATGCCAAATGCTTCAAGTGAGAAATAGACAGAACATCATTTGTTGTTGGGCTAGAGGACTGACTTGGGAGGAAGGTTTCTGTGGACATTTTGGGTCGATTTTGTTTTCTGCCGTCTTTTGAAGTAGATTTTGTCTTTTATTTATTATCTGAGGCAAACTGAACACACCCTTCTTTGATTTTCTATTTAAATTTCAAGCCATTTAGCTTAGCCAGTGCTGTTATGATTTATAAAATCCACTTTCCTCCCTTTTTAGTTTATCCATTACGACTTAGCTTTTTTTTTTCTTTTTAAGAAGCAAGATTGTCCAAATTTAATTTGAGCCTTTTAGTTTGGGCAATTTCTTGAATATCTAAATGAGTGCGCATGTATTTNTTTTT

mRNA sequence

TAGAACAAAATTAGGCTGGGTGTGTCGGGCCATGGAGCCCAAAGACGAATACCCGATCCTGAAACACCTTTAAAACCCTAGTTTTTGCTCATTATAACACACCTCCCGCAGCAGGCTTACTCTTGCTATCGCTCGCCCCCAGATCTGTATTCTTTGAGCGAAACGTTTTTTCTACAGCGAAGCGATGGCTCTGGTTCTGTACTCCTGGAACACTAACAAAAATGCTTACAAAGCTCTTATAGTTGCAGAATACAATGGTGTCAAGGTTGACCTTGCCCCAGGTTTTACCATGGGCGTAACCAATAAGTCTCCTGAGTTTCTCAAAATGAATCCTATTGGGAAGGTTCCTGTGCTTGAAACACCCGAAGGTGGTATCTTTGAGAGCAATGCCATAGCTCGCTATGTTGCTCGCTTGAAGGACTGTGGTTTATTTGGTTCTTCTCCAATTGAATATGGACATGTTGAACAATGGATTGACTTTGCCACACTTGAAATTGATGTTCATATTGGTGCTTTAGCAGGGCCTAGATATGGATATCGTGCTTACCATGCTGCTGTTGAAGAAGCTGCAAACGCTGCCCTAAAACGCTCTTTTGCTGCTCTGAACTCTTACCTTGCTTCAAACACATACCTTGTTGGACATTCTGTGACACTGGCTGATATCATTGTAACATGCAATTTGTATTGGGGAATCTCTTTTCTGTTGACTAAAAGCTTTACTTCTGAGTTCCCTCACGTCGAGAGGTACTTCTGGACAATGGTCAATCAACCAAATTTCAAGAAGATTATTGGTGAGTTTAAGCAGACAGAAACTGTTCCACCAGTGAAAACACCGGGAGAGGCTGCAGCAGCTGCCAAGCCAAAGGCTGAGCCTAAGAAGGAAGAGAAGCCCAAAGCAGCAGCACCTGCAGAGGAAGAGGATGCACCAAAGCCCAAGGCCAAAAATCCTCTTGACCTCCTTCCTCCCAGTAAGATGATTTTGGATGAGTGGAAACGTCTCTACTCGAACACAAAAACTAACTTCCGTGAGGTTGCAATCAAAGGATTCTGGGACATGTACGATCCTGAAGGGTACTCCCTTTGGTTCTGTGATTACAAGTACAATGACGAGAACACAGTTTCCTTTGTGACTTTGAACAAAGTCAGCGGATTTCTCCAGAGAATGGATCTTGCTCGTAAATATGCCTTTGGAAAGATGCTTGTGATCGGATCAGAGGCACCCTTCAAGGTGAAGGGGCTATGGCTTTTCCGTGGGCAAGAAATCCCACAATTTGTTATGGACGAGTGCTATGACATGGAGCTGTACGAGTGGAAGAAGGTCGATATCTCCGATGAAGCCCAGAAGGAGAGAGTTAACCAAATGATTGAAGATCAGGAACCATTTGAGGGAGAGGCTCTCTTGGATGCCAAATGCTTCAAGTGAGAAATAGACAGAACATCATTTGTTGTTGGGCTAGAGGACTGACTTGGGAGGAAGGTTTCTGTGGACATTTTGGGTCGATTTTGTTTTCTGCCGTCTTTTGAAGTAGATTTTGTCTTTTATTTATTATCTGAGGCAAACTGAACACACCCTTCTTTGATTTTCTATTTAAATTTCAAGCCATTTAGCTTAGCCAGTGCTGTTATGATTTATAAAATCCACTTTCCTCCCTTTTTAGTTTATCCATTACGACTTAGCTTTTTTTTTTCTTTTTAAGAAGCAAGATTGTCCAAATTTAATTTGAGCCTTTTAGTTTGGGCAATTTCTTGAATATCTAAATGAGTGCGCATGTATTTNTTTTT

Coding sequence (CDS)

ATGGCTCTGGTTCTGTACTCCTGGAACACTAACAAAAATGCTTACAAAGCTCTTATAGTTGCAGAATACAATGGTGTCAAGGTTGACCTTGCCCCAGGTTTTACCATGGGCGTAACCAATAAGTCTCCTGAGTTTCTCAAAATGAATCCTATTGGGAAGGTTCCTGTGCTTGAAACACCCGAAGGTGGTATCTTTGAGAGCAATGCCATAGCTCGCTATGTTGCTCGCTTGAAGGACTGTGGTTTATTTGGTTCTTCTCCAATTGAATATGGACATGTTGAACAATGGATTGACTTTGCCACACTTGAAATTGATGTTCATATTGGTGCTTTAGCAGGGCCTAGATATGGATATCGTGCTTACCATGCTGCTGTTGAAGAAGCTGCAAACGCTGCCCTAAAACGCTCTTTTGCTGCTCTGAACTCTTACCTTGCTTCAAACACATACCTTGTTGGACATTCTGTGACACTGGCTGATATCATTGTAACATGCAATTTGTATTGGGGAATCTCTTTTCTGTTGACTAAAAGCTTTACTTCTGAGTTCCCTCACGTCGAGAGGTACTTCTGGACAATGGTCAATCAACCAAATTTCAAGAAGATTATTGGTGAGTTTAAGCAGACAGAAACTGTTCCACCAGTGAAAACACCGGGAGAGGCTGCAGCAGCTGCCAAGCCAAAGGCTGAGCCTAAGAAGGAAGAGAAGCCCAAAGCAGCAGCACCTGCAGAGGAAGAGGATGCACCAAAGCCCAAGGCCAAAAATCCTCTTGACCTCCTTCCTCCCAGTAAGATGATTTTGGATGAGTGGAAACGTCTCTACTCGAACACAAAAACTAACTTCCGTGAGGTTGCAATCAAAGGATTCTGGGACATGTACGATCCTGAAGGGTACTCCCTTTGGTTCTGTGATTACAAGTACAATGACGAGAACACAGTTTCCTTTGTGACTTTGAACAAAGTCAGCGGATTTCTCCAGAGAATGGATCTTGCTCGTAAATATGCCTTTGGAAAGATGCTTGTGATCGGATCAGAGGCACCCTTCAAGGTGAAGGGGCTATGGCTTTTCCGTGGGCAAGAAATCCCACAATTTGTTATGGACGAGTGCTATGACATGGAGCTGTACGAGTGGAAGAAGGTCGATATCTCCGATGAAGCCCAGAAGGAGAGAGTTAACCAAATGATTGAAGATCAGGAACCATTTGAGGGAGAGGCTCTCTTGGATGCCAAATGCTTCAAGTGA

Protein sequence

MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETPEGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRAYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTSEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAAPAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Homology
BLAST of Cp4.1LG01g08540 vs. ExPASy Swiss-Prot
Match: Q9FUM1 (Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 4.4e-180
Identity = 322/422 (76.30%), Postives = 359/422 (85.07%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL++  TNKNA+K LIVAEY GVKV+LAP F MGVTNK+PE+LK+NPIGKVP+LETP
Sbjct: 1   MALVLHAGKTNKNAFKTLIVAEYTGVKVELAPDFEMGVTNKTPEYLKLNPIGKVPLLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           +G IFESNAIARYVARLK D  L GSS I+Y H+EQWIDF +LEID +I +   PR+GY 
Sbjct: 61  DGPIFESNAIARYVARLKADNPLIGSSLIDYAHIEQWIDFGSLEIDANIISWFRPRFGYA 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y    EEAA +ALKR+  ALN++LASNTYLVGH VTLADIIVTCNL++G + L+ KSFT
Sbjct: 121 VYLPPAEEAAISALKRALGALNTHLASNTYLVGHFVTLADIIVTCNLFFGFTKLMIKSFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKT------PGEAAAAAK--PKAEPK 240
           SEFPHVERYFWT+VNQP FKK++G+ KQTE+VPPV +      P E  + AK  PK E K
Sbjct: 181 SEFPHVERYFWTLVNQPKFKKVLGDVKQTESVPPVPSAKKPSQPKETKSKAKEEPKKEAK 240

Query: 241 KE-EKPKAAAPAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           KE  KPKA A  E E+APKPK KNPLDLLPPS M+LD+WKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 KEPAKPKAEAAEEVEEAPKPKPKNPLDLLPPSNMVLDDWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVK 360
           MYDPEGYSLWFC+YKYNDENTVSFVTLNKV GFLQRMDLARKYAFGKMLVIGSE PFKVK
Sbjct: 301 MYDPEGYSLWFCEYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKC 413
           GLWLFRGQEIP FVM+ECYDMELY W KVD+SDE QKERVNQ+IEDQEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPPFVMEECYDMELYNWTKVDLSDENQKERVNQVIEDQEPFEGEALLDAKC 420

BLAST of Cp4.1LG01g08540 vs. ExPASy Swiss-Prot
Match: Q5Z627 (Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0571400 PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.8e-178
Identity = 314/417 (75.30%), Postives = 351/417 (84.17%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+  + NKNA+KALI AEY GVKV+L   F MGV+NK+PEFLKMNP+GK+PVLETP
Sbjct: 1   MALVLHCGSGNKNAFKALIAAEYTGVKVELTKNFEMGVSNKTPEFLKMNPLGKIPVLETP 60

Query: 61  EGGIFESNAIARYVARLKD-CGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG +FESNAIARYVARLKD   L GSS I+Y H+EQW+DF+  E+D +IG    PR G+ 
Sbjct: 61  EGAVFESNAIARYVARLKDNSSLCGSSLIDYSHIEQWMDFSATEVDANIGRWLYPRLGFG 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y   +EE A  +LKRS  ALN++LASNTYLVGHSVTLADI++TCNLY+G   +L KSFT
Sbjct: 121 PYVPVLEEFAITSLKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYYGFVRILIKSFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVK----TPGEAAAAAKPKAEPKKEEK 240
           SEFPHVERYFWTMVNQPNFKK+IG+FKQ E+VPPV+     P E+ A    K  PK+  K
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVIGDFKQAESVPPVQKKAAPPKESKAKEAKKEAPKEAPK 240

Query: 241 PKAAAPAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPE 300
           PK  A +EEE+APKPK KNPLDLLPPSKMILDEWKRLYSNTKTNFRE+AIKGFWDMYDPE
Sbjct: 241 PKVEA-SEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREIAIKGFWDMYDPE 300

Query: 301 GYSLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLF 360
           GYSLWFCDYKYNDENTVSFVT+NKV GFLQRMDL RKYAFGKMLVIGS  PFKVKGLWLF
Sbjct: 301 GYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSTPPFKVKGLWLF 360

Query: 361 RGQEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           RGQ+IP+FVMDE YDMELYEW KVD+SDEAQKERVN MIEDQEPFEGE LLDAKCFK
Sbjct: 361 RGQDIPKFVMDEVYDMELYEWTKVDLSDEAQKERVNAMIEDQEPFEGEDLLDAKCFK 416

BLAST of Cp4.1LG01g08540 vs. ExPASy Swiss-Prot
Match: Q9ZRI7 (Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220600 PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 2.4e-178
Identity = 316/418 (75.60%), Postives = 354/418 (84.69%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL++++ NKNA+KALI AEY+GVKV+LA  F MGV+NK+PE+LKMNPIGKVP+LETP
Sbjct: 1   MALVLHTFDGNKNAFKALIAAEYSGVKVELAKNFQMGVSNKTPEYLKMNPIGKVPILETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           +G +FESNAIARYV R K D  L+GSS IEY H+EQWIDF+  E+D + G    PR G+ 
Sbjct: 61  DGPVFESNAIARYVTRSKSDNPLYGSSLIEYAHIEQWIDFSATEVDANTGKWLFPRLGFA 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y A  EEAA AALKRS  ALN++LASNTYLVGHSVTLADI++TCNLY G + ++TK+FT
Sbjct: 121 PYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKNFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKP----KAEPKKEEK 240
           SEFPHVERYFWTMVNQPNFKK++G+ KQ ++VP V+    A    KP    K  PK+  K
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVMGDVKQADSVPQVQKKAAAPKEQKPKEAKKEAPKEAPK 240

Query: 241 PKAA-APAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP 300
           PKAA  P EEE+APKPK KNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP
Sbjct: 241 PKAAEKPEEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP 300

Query: 301 EGYSLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWL 360
           EGYSLWFCDYKYNDENTVSFVT+NKV GFLQRMDL RKYAFGKMLVIGSE PFKVKGLWL
Sbjct: 301 EGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFKVKGLWL 360

Query: 361 FRGQEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           FRG EIP+FVMDE YDMELYEW KVDISDEAQKERV+ MIED EPFEGEALLDAKCFK
Sbjct: 361 FRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGEALLDAKCFK 418

BLAST of Cp4.1LG01g08540 vs. ExPASy Swiss-Prot
Match: Q6YW46 (Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220500 PE=2 SV=2)

HSP 1 Score: 623.6 bits (1607), Expect = 1.6e-177
Identity = 316/418 (75.60%), Postives = 350/418 (83.73%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL++ + NKNA+KALI AEY+GVKV+L   F MGV+NK+PEFLKMNPIGK+PVLETP
Sbjct: 1   MALVLHAGSGNKNAFKALIAAEYSGVKVELVKNFQMGVSNKTPEFLKMNPIGKIPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           +G +FESNAIARYV R K D  L+GSS IEY H+EQW DF+  E+D +IG    PR G  
Sbjct: 61  DGPVFESNAIARYVTRSKADNPLYGSSLIEYAHIEQWNDFSATEVDANIGKWLYPRLGIA 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y A  EEAA AALKRS  ALN++LASNTYLVGHSVTLADI++TCNLY G + ++TKSFT
Sbjct: 121 PYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKSFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKP----KAEPKKEEK 240
           SEFPHVERYFWTMVNQPNFKK++G+ KQ E+VPPV+         KP    K  PK+  K
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVLGDVKQAESVPPVQKKAPPPKEQKPKEAKKEAPKEAPK 240

Query: 241 PKAA-APAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP 300
           PKA   P EEE+APKPK KNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP
Sbjct: 241 PKAVEKPEEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDP 300

Query: 301 EGYSLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWL 360
           EGYSLWFCDYKYNDENTVSFVT+NKV GFLQRMDL RKYAFGKMLVIGSE PFKVKGLWL
Sbjct: 301 EGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFKVKGLWL 360

Query: 361 FRGQEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           FRG EIP+FVMDE YDMELYEW KVDISDEAQKERV+ MIED EPFEGE+LLDAKCFK
Sbjct: 361 FRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGESLLDAKCFK 418

BLAST of Cp4.1LG01g08540 vs. ExPASy Swiss-Prot
Match: O04487 (Probable elongation factor 1-gamma 1 OS=Arabidopsis thaliana OX=3702 GN=At1g09640 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.1e-173
Identity = 308/415 (74.22%), Postives = 347/415 (83.61%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+++  NK+A KALI AEY GV++D+   F MGVTNK+P FLKMNPIGKVPVLETP
Sbjct: 1   MALVLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG +FESNAIARYV+RL  D  L GSS IEY  +EQWIDF++LEI   I    GPR G+ 
Sbjct: 61  EGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFM 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y A  EE A + LKR+  ALN++L SNTYLVGHS+TLADII  CNL  G + ++TK FT
Sbjct: 121 PYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAA 240
           SEFPHVERYFWT+VNQPNF K++G+ KQTE VPP+ +  +AA  AKPK EPKK+E P A 
Sbjct: 181 SEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIASK-KAAQPAKPKEEPKKKEAPVAE 240

Query: 241 AP--AEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGY 300
           AP  AEEE+APKPKAKNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFWDMYDPEGY
Sbjct: 241 APKLAEEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGY 300

Query: 301 SLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRG 360
           SLWFCDYKYNDEN VSFVTLNKV GFLQRMDLARKY+FGKML+ GSE PFKVKGLWLFRG
Sbjct: 301 SLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRG 360

Query: 361 QEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
            EIP+F+MDE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKCFK
Sbjct: 361 PEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKCFK 414

BLAST of Cp4.1LG01g08540 vs. NCBI nr
Match: XP_022942559.1 (elongation factor 1-gamma-like [Cucurbita moschata] >XP_023542753.1 elongation factor 1-gamma-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821 bits (2121), Expect = 4.30e-300
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA
Sbjct: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS
Sbjct: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA
Sbjct: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. NCBI nr
Match: XP_022984327.1 (elongation factor 1-gamma-like [Cucurbita maxima] >KAG6600716.1 Elongation factor 1-gamma 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031354.1 Elongation factor 1-gamma 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 819 bits (2116), Expect = 2.49e-299
Identity = 411/412 (99.76%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA
Sbjct: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS
Sbjct: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPK EPKKEEKPKAAA
Sbjct: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKPEPKKEEKPKAAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. NCBI nr
Match: XP_022929411.1 (elongation factor 1-gamma [Cucurbita moschata] >XP_022929412.1 elongation factor 1-gamma [Cucurbita moschata])

HSP 1 Score: 727 bits (1877), Expect = 6.56e-263
Identity = 361/412 (87.62%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW  NKNAYKA I AEYNGVK+DL+P F MGVTNKSPE+LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKANKNAYKAFIAAEYNGVKIDLSPDFVMGVTNKSPEYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD  LFGSS I+YGHVEQWIDF+T EID HI  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSSLFGSSSIDYGHVEQWIDFSTTEIDAHISTILRPRFGYGV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YH AVEEAANAALKRSFAALNSYLASNT+LVGHSVTLADII+TCNLY G +++LTKSFTS
Sbjct: 121 YHPAVEEAANAALKRSFAALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYILTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNF+KIIGE KQT+ +PPVKTP EAAAAAKPK EPKK+E+   AA
Sbjct: 181 EFPHVERYFWTLVNQPNFQKIIGEVKQTDAIPPVKTPEEAAAAAKPKPEPKKQEEKPKAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA EE+APKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAAEEEAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVIDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. NCBI nr
Match: XP_023552554.1 (elongation factor 1-gamma [Cucurbita pepo subsp. pepo] >XP_023552555.1 elongation factor 1-gamma [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727 bits (1876), Expect = 9.32e-263
Identity = 360/412 (87.38%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW  NKNAYKA I AEYNGVK+DL+P F MGVTNKSPE+LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKANKNAYKAFIAAEYNGVKIDLSPDFVMGVTNKSPEYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD  LFGSS I+YGHVEQWIDF+T E+D HI  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSSLFGSSSIDYGHVEQWIDFSTTELDAHISTILRPRFGYGV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YH AVEEAANAALKRSFAALNSYLASNT+LVGHSVTLADII+TCNLY G +++LTKSFTS
Sbjct: 121 YHPAVEEAANAALKRSFAALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYILTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNF+KIIGE KQT+ +PPVKTP EAAAAAKPK EPKK+E+   AA
Sbjct: 181 EFPHVERYFWTLVNQPNFRKIIGEVKQTDAIPPVKTPEEAAAAAKPKPEPKKQEEKPKAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA EE+APKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAAEEEAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVIDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. NCBI nr
Match: XP_022984659.1 (elongation factor 1-gamma [Cucurbita maxima] >XP_022984660.1 elongation factor 1-gamma [Cucurbita maxima])

HSP 1 Score: 724 bits (1870), Expect = 7.65e-262
Identity = 359/412 (87.14%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW  NKNAYKA I AEYNGVK+DL+P F MGVTNKSP +LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKANKNAYKAFIAAEYNGVKIDLSPDFVMGVTNKSPVYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD  LFGSS I+YGHVEQWIDF+T+EID HI  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSSLFGSSSIDYGHVEQWIDFSTMEIDAHISTILRPRFGYGV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           +H AVEEAANAALKRSFAALNSYLASNT+LVGHSVTLADII+TCNLY G +++LTKSFTS
Sbjct: 121 FHPAVEEAANAALKRSFAALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYILTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNF+KIIGE KQT+ +PPVKTP EAAAAAKPK EPKK+E+   AA
Sbjct: 181 EFPHVERYFWTLVNQPNFRKIIGEVKQTDAIPPVKTPEEAAAAAKPKPEPKKQEEKPKAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA EE+APKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAAEEEAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVIDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. ExPASy TrEMBL
Match: A0A6J1FRN7 (elongation factor 1-gamma-like OS=Cucurbita moschata OX=3662 GN=LOC111447557 PE=4 SV=1)

HSP 1 Score: 821 bits (2121), Expect = 2.08e-300
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA
Sbjct: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS
Sbjct: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA
Sbjct: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. ExPASy TrEMBL
Match: A0A6J1J1W6 (elongation factor 1-gamma-like OS=Cucurbita maxima OX=3661 GN=LOC111482661 PE=4 SV=1)

HSP 1 Score: 819 bits (2116), Expect = 1.20e-299
Identity = 411/412 (99.76%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA
Sbjct: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS
Sbjct: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPK EPKKEEKPKAAA
Sbjct: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKPEPKKEEKPKAAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. ExPASy TrEMBL
Match: A0A6J1EMQ4 (elongation factor 1-gamma OS=Cucurbita moschata OX=3662 GN=LOC111435990 PE=4 SV=1)

HSP 1 Score: 727 bits (1877), Expect = 3.18e-263
Identity = 361/412 (87.62%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW  NKNAYKA I AEYNGVK+DL+P F MGVTNKSPE+LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKANKNAYKAFIAAEYNGVKIDLSPDFVMGVTNKSPEYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD  LFGSS I+YGHVEQWIDF+T EID HI  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSSLFGSSSIDYGHVEQWIDFSTTEIDAHISTILRPRFGYGV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YH AVEEAANAALKRSFAALNSYLASNT+LVGHSVTLADII+TCNLY G +++LTKSFTS
Sbjct: 121 YHPAVEEAANAALKRSFAALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYILTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNF+KIIGE KQT+ +PPVKTP EAAAAAKPK EPKK+E+   AA
Sbjct: 181 EFPHVERYFWTLVNQPNFQKIIGEVKQTDAIPPVKTPEEAAAAAKPKPEPKKQEEKPKAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA EE+APKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAAEEEAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVIDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. ExPASy TrEMBL
Match: A0A6J1J972 (elongation factor 1-gamma OS=Cucurbita maxima OX=3661 GN=LOC111482875 PE=4 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 3.70e-262
Identity = 359/412 (87.14%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW  NKNAYKA I AEYNGVK+DL+P F MGVTNKSP +LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKANKNAYKAFIAAEYNGVKIDLSPDFVMGVTNKSPVYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD  LFGSS I+YGHVEQWIDF+T+EID HI  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSSLFGSSSIDYGHVEQWIDFSTMEIDAHISTILRPRFGYGV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           +H AVEEAANAALKRSFAALNSYLASNT+LVGHSVTLADII+TCNLY G +++LTKSFTS
Sbjct: 121 FHPAVEEAANAALKRSFAALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYILTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNF+KIIGE KQT+ +PPVKTP EAAAAAKPK EPKK+E+   AA
Sbjct: 181 EFPHVERYFWTLVNQPNFRKIIGEVKQTDAIPPVKTPEEAAAAAKPKPEPKKQEEKPKAA 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA EE+APKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW
Sbjct: 241 PAAEEEAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVIDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412

BLAST of Cp4.1LG01g08540 vs. ExPASy TrEMBL
Match: A0A5A7UZA7 (Elongation factor 1-gamma-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G001000 PE=4 SV=1)

HSP 1 Score: 709 bits (1830), Expect = 4.26e-256
Identity = 356/412 (86.41%), Postives = 381/412 (92.48%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+SW TNKNA+KALI AEYNGVKVDL+P FTMGVTNKSPE+LKMNPIGKVPVLETP
Sbjct: 1   MALVLHSWKTNKNAFKALIAAEYNGVKVDLSPDFTMGVTNKSPEYLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYRA 120
           +G IFESNAIARYVARLKD GLFGSS I+ GHVEQWIDF+T EID ++  +  PR+GY  
Sbjct: 61  DGAIFESNAIARYVARLKDSGLFGSSSIDCGHVEQWIDFSTFEIDNNLSVILRPRFGYAV 120

Query: 121 YHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFTS 180
           YH  VEE ANAALKRSF ALNSYLASNT+LVGHSVTLADII+TCNLY G ++LLTKSFTS
Sbjct: 121 YHPGVEETANAALKRSFGALNSYLASNTFLVGHSVTLADIILTCNLYLGFTYLLTKSFTS 180

Query: 181 EFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAAA 240
           EFPHVERYFWT+VNQPNFKKIIGE KQT+ VPPVKTP EAAAAAK KAEPKK+E+ K A 
Sbjct: 181 EFPHVERYFWTLVNQPNFKKIIGEVKQTDAVPPVKTPEEAAAAAKAKAEPKKQEE-KPAE 240

Query: 241 PAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLW 300
           PA+EE APKPKAKNPLDLLPPS MILDEWKRLYSNTKTNFRE+AIKGFWDMYDPEGYSLW
Sbjct: 241 PAQEE-APKPKAKNPLDLLPPSSMILDEWKRLYSNTKTNFREIAIKGFWDMYDPEGYSLW 300

Query: 301 FCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQEI 360
           FCDYKYNDENTVSFVTLNKV GFLQRMD+ARKYAFGKMLVIGSEAP+KVKGLWLFRG+EI
Sbjct: 301 FCDYKYNDENTVSFVTLNKVGGFLQRMDIARKYAFGKMLVIGSEAPYKVKGLWLFRGKEI 360

Query: 361 PQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 412
           PQFV+DECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK
Sbjct: 361 PQFVLDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 410

BLAST of Cp4.1LG01g08540 vs. TAIR 10
Match: AT1G09640.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 609.4 bits (1570), Expect = 2.2e-174
Identity = 308/415 (74.22%), Postives = 347/415 (83.61%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+++  NK+A KALI AEY GV++D+   F MGVTNK+P FLKMNPIGKVPVLETP
Sbjct: 1   MALVLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG +FESNAIARYV+RL  D  L GSS IEY  +EQWIDF++LEI   I    GPR G+ 
Sbjct: 61  EGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFM 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y A  EE A + LKR+  ALN++L SNTYLVGHS+TLADII  CNL  G + ++TK FT
Sbjct: 121 PYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAA 240
           SEFPHVERYFWT+VNQPNF K++G+ KQTE VPP+ +  +AA  AKPK EPKK+E P A 
Sbjct: 181 SEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIASK-KAAQPAKPKEEPKKKEAPVAE 240

Query: 241 AP--AEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGY 300
           AP  AEEE+APKPKAKNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFWDMYDPEGY
Sbjct: 241 APKLAEEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGY 300

Query: 301 SLWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRG 360
           SLWFCDYKYNDEN VSFVTLNKV GFLQRMDLARKY+FGKML+ GSE PFKVKGLWLFRG
Sbjct: 301 SLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRG 360

Query: 361 QEIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
            EIP+F+MDE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKCFK
Sbjct: 361 PEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKCFK 414

BLAST of Cp4.1LG01g08540 vs. TAIR 10
Match: AT1G57720.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 607.4 bits (1565), Expect = 8.2e-174
Identity = 309/414 (74.64%), Postives = 345/414 (83.33%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALV++++  NK A KALI AEY GVK++ +  F MGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG IFESNAIARYV+R   D  L GSS IEY H+EQWIDF++LEID ++     PR GY 
Sbjct: 61  EGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYA 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            + A  EEAA +ALKR   ALN++LASNT+LVGHSVTLADI+  CNL  G + ++TK FT
Sbjct: 121 PFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEK-PKA 240
           S FPHVERYFWTMVNQP FKK++G+ KQTE VPPV T  +A   AKPK EPKK     +A
Sbjct: 181 SAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTK-KAPQPAKPKEEPKKAAPVAEA 240

Query: 241 AAPAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYS 300
             PAEEE+APKPKAKNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFWDMYDPEGYS
Sbjct: 241 PKPAEEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYS 300

Query: 301 LWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQ 360
           LWFCDYKYNDEN VSFVTLNKV GFLQRMDLARKY+FGKML+ GSE PFKVKGLWLFRG 
Sbjct: 301 LWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGP 360

Query: 361 EIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           EIP+F+MDE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKCFK
Sbjct: 361 EIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKCFK 413

BLAST of Cp4.1LG01g08540 vs. TAIR 10
Match: AT1G57720.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 607.4 bits (1565), Expect = 8.2e-174
Identity = 309/414 (74.64%), Postives = 345/414 (83.33%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALV++++  NK A KALI AEY GVK++ +  F MGVTNKSPEFLKMNPIGKVPVLETP
Sbjct: 1   MALVMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG IFESNAIARYV+R   D  L GSS IEY H+EQWIDF++LEID ++     PR GY 
Sbjct: 61  EGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYA 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            + A  EEAA +ALKR   ALN++LASNT+LVGHSVTLADI+  CNL  G + ++TK FT
Sbjct: 121 PFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEK-PKA 240
           S FPHVERYFWTMVNQP FKK++G+ KQTE VPPV T  +A   AKPK EPKK     +A
Sbjct: 181 SAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTK-KAPQPAKPKEEPKKAAPVAEA 240

Query: 241 AAPAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYS 300
             PAEEE+APKPKAKNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFWDMYDPEGYS
Sbjct: 241 PKPAEEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYS 300

Query: 301 LWFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQ 360
           LWFCDYKYNDEN VSFVTLNKV GFLQRMDLARKY+FGKML+ GSE PFKVKGLWLFRG 
Sbjct: 301 LWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGP 360

Query: 361 EIPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           EIP+F+MDE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKCFK
Sbjct: 361 EIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKCFK 413

BLAST of Cp4.1LG01g08540 vs. TAIR 10
Match: AT1G09640.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 315.5 bits (807), Expect = 6.5e-86
Identity = 189/413 (45.76%), Postives = 215/413 (52.06%), Query Frame = 0

Query: 1   MALVLYSWNTNKNAYKALIVAEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPVLETP 60
           MALVL+++  NK+A KALI AEY GV++D+   F MGVTNK+P FLKMNPIGKVPVLETP
Sbjct: 1   MALVLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETP 60

Query: 61  EGGIFESNAIARYVARLK-DCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALAGPRYGYR 120
           EG +FESNAIARYV+RL  D  L GSS IEY  +EQWIDF++LEI   I    GPR G+ 
Sbjct: 61  EGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFM 120

Query: 121 AYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADIIVTCNLYWGISFLLTKSFT 180
            Y A  EE A + LKR+  ALN++L SNTYLVGHS+TLADII  CNL  G + ++TK FT
Sbjct: 121 PYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTMVNQPNFKKIIGEFKQTETVPPVKTPGEAAAAAKPKAEPKKEEKPKAA 240
           SEFPHVERYFWT+VNQPNF                                         
Sbjct: 181 SEFPHVERYFWTVVNQPNFT---------------------------------------- 240

Query: 241 APAEEEDAPKPKAKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSL 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 265

Query: 301 WFCDYKYNDENTVSFVTLNKVSGFLQRMDLARKYAFGKMLVIGSEAPFKVKGLWLFRGQE 360
                                                           KVKGLWLFRG E
Sbjct: 301 ------------------------------------------------KVKGLWLFRGPE 265

Query: 361 IPQFVMDECYDMELYEWKKVDISDEAQKERVNQMIEDQEPFEGEALLDAKCFK 413
           IP+F+MDE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKCFK
Sbjct: 361 IPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKCFK 265

BLAST of Cp4.1LG01g08540 vs. TAIR 10
Match: AT2G30870.1 (glutathione S-transferase PHI 10 )

HSP 1 Score: 69.3 bits (168), Expect = 8.1e-12
Identity = 55/172 (31.98%), Postives = 80/172 (46.51%), Query Frame = 0

Query: 1   MALVLYS--WNTNKNAYKALIV--AEYNGVKVDLAPGFTMGVTNKSPEFLKMNPIGKVPV 60
           M L +Y+  + ++K A   L+     +  V VDL  G       + PE+L + P GK+PV
Sbjct: 1   MVLTIYAPLFASSKRAVVTLVEKGVSFETVNVDLMKG-----EQRQPEYLAIQPFGKIPV 60

Query: 61  LETPEGGIFESNAIARYVA---RLKDCGLFGSSPIEYGHVEQWIDFATLEIDVHIGALA- 120
           L   +  IFES AI RY+A   R +   L G +  E G VEQW+D         + AL  
Sbjct: 61  LVDGDYKIFESRAIMRYIAEKYRSQGPDLLGKTIEERGQVEQWLDVEATSYHPPLLALTL 120

Query: 121 ----GPRYGYRAYHAAVEEAANAALKRSFAALNSYLASNTYLVGHSVTLADI 161
                P  G+ A    ++E +   L        + L+ N YL G  V+LAD+
Sbjct: 121 NIVFAPLMGFPADEKVIKE-SEEKLAEVLDVYEAQLSKNEYLAGDFVSLADL 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FUM14.4e-18076.30Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1[more]
Q5Z6271.8e-17875.30Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g057... [more]
Q9ZRI72.4e-17875.60Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022... [more]
Q6YW461.6e-17775.60Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022... [more]
O044873.1e-17374.22Probable elongation factor 1-gamma 1 OS=Arabidopsis thaliana OX=3702 GN=At1g0964... [more]
Match NameE-valueIdentityDescription
XP_022942559.14.30e-300100.00elongation factor 1-gamma-like [Cucurbita moschata] >XP_023542753.1 elongation f... [more]
XP_022984327.12.49e-29999.76elongation factor 1-gamma-like [Cucurbita maxima] >KAG6600716.1 Elongation facto... [more]
XP_022929411.16.56e-26387.62elongation factor 1-gamma [Cucurbita moschata] >XP_022929412.1 elongation factor... [more]
XP_023552554.19.32e-26387.38elongation factor 1-gamma [Cucurbita pepo subsp. pepo] >XP_023552555.1 elongatio... [more]
XP_022984659.17.65e-26287.14elongation factor 1-gamma [Cucurbita maxima] >XP_022984660.1 elongation factor 1... [more]
Match NameE-valueIdentityDescription
A0A6J1FRN72.08e-300100.00elongation factor 1-gamma-like OS=Cucurbita moschata OX=3662 GN=LOC111447557 PE=... [more]
A0A6J1J1W61.20e-29999.76elongation factor 1-gamma-like OS=Cucurbita maxima OX=3661 GN=LOC111482661 PE=4 ... [more]
A0A6J1EMQ43.18e-26387.62elongation factor 1-gamma OS=Cucurbita moschata OX=3662 GN=LOC111435990 PE=4 SV=... [more]
A0A6J1J9723.70e-26287.14elongation factor 1-gamma OS=Cucurbita maxima OX=3661 GN=LOC111482875 PE=4 SV=1[more]
A0A5A7UZA74.26e-25686.41Elongation factor 1-gamma-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT1G09640.12.2e-17474.22Translation elongation factor EF1B, gamma chain [more]
AT1G57720.18.2e-17474.64Translation elongation factor EF1B, gamma chain [more]
AT1G57720.28.2e-17474.64Translation elongation factor EF1B, gamma chain [more]
AT1G09640.26.5e-8645.76Translation elongation factor EF1B, gamma chain [more]
AT2G30870.18.1e-1231.98glutathione S-transferase PHI 10 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001662Elongation factor 1B gamma, C-terminalSMARTSM01183EF1G_2coord: 251..359
e-value: 9.3E-67
score: 237.8
IPR001662Elongation factor 1B gamma, C-terminalPFAMPF00647EF1Gcoord: 251..359
e-value: 1.8E-41
score: 140.5
IPR001662Elongation factor 1B gamma, C-terminalPROSITEPS50040EF1G_Ccoord: 252..412
score: 81.430305
IPR004045Glutathione S-transferase, N-terminalPFAMPF02798GST_Ncoord: 1..76
e-value: 5.9E-16
score: 58.6
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 1..82
score: 20.390261
IPR036433Elongation factor EF1B gamma, C-terminal domain superfamilyGENE3D3.30.70.1010Translation elongation factor EF1B, gamma chain, conserved domaincoord: 252..412
e-value: 2.7E-70
score: 237.1
IPR036433Elongation factor EF1B gamma, C-terminal domain superfamilySUPERFAMILY89942eEF1-gamma domaincoord: 253..412
IPR004046Glutathione S-transferase, C-terminalPFAMPF00043GST_Ccoord: 122..196
e-value: 1.5E-11
score: 44.4
NoneNo IPR availableGENE3D1.20.1050.10coord: 80..213
e-value: 1.9E-38
score: 133.5
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 1..74
e-value: 2.1E-22
score: 81.1
NoneNo IPR availableSFLDSFLDG00358Main_(cytGST)coord: 3..192
e-value: 9.9E-31
score: 101.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..253
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..252
NoneNo IPR availablePANTHERPTHR44372:SF5SUBFAMILY NOT NAMEDcoord: 1..412
NoneNo IPR availableCDDcd03181GST_C_EF1Bgamma_likecoord: 89..211
e-value: 1.75213E-44
score: 148.863
NoneNo IPR availableCDDcd03044GST_N_EF1Bgammacoord: 3..77
e-value: 1.62868E-25
score: 96.9395
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 3..192
e-value: 9.9E-31
score: 101.7
IPR044628Elongation factor 1-gamma, plantPANTHERPTHR44372ELONGATION FACTOR 1-GAMMA 1-RELATEDcoord: 1..412
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 86..214
score: 22.637051
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 73..213
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 2..77

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g08540.1Cp4.1LG01g08540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0006414 translational elongation
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003746 translation elongation factor activity