Cp4.1LG01g08430 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g08430
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiontranscription factor bHLH123-like isoform X2
LocationCp4.1LG01: 5005140 .. 5007045 (+)
RNA-Seq ExpressionCp4.1LG01g08430
SyntenyCp4.1LG01g08430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTTCTGATTTTAATTTTGTTTATTTTATTTTAGTTGCAGTGCAAGCAATATAAATAGCGATATGCCGCGAAGCGTTTTCAATCGTTTTTTTTTGTAAATAGAAATTCCACACACGAACACAGAACAAATCCGAAAGAACCAAATCCCAAATCATCAAACTGGGTTTGAGATTCAGAGCTGCAGTAGCCATGGCGGAGGAGTTTCAGAGCACTGGAAACTGGTGGGACGCTTCTAGAAGCCGCTACGAAGCAGGAATATCCCCTTCTTCCTCCGCCATAACTACTTTCGTCGACCACCCGGACTCCGGCGCCGGCGCAGGCGACCCCAATTTGCACATCATGGGCTTGGGCCTCGATTGGAACCAACCCCTCTTGTAAGTGTAATCCCTTTTGCTTTTTAATTTACGGCGGCTATGGGTATTAATTTTTTGTTGTTTTTGGGACAGTCGGGGCGGCGGAGAGAAGGCGGCGGAGAGCAGTTTCCGGTCGATGCTGCAGCCGGATAATATGAATTTGAATATGCAAGAAACAGGGCAACACCACCAACACCAACAACAACAGATTCAATGGATGAGATCGGAGAAGCTATATTCAGGGGAATCTCCAGCGACTGAATTCAAGCCAATAAACAGAGGGTTTTCACTAGATCACCACAACCAGCCTCCGCCACAGTTCAGCCCTTCTCATTACAGCACCGGCGACAGCACCATCACCAGTTTTCCAATCGACACCGCCGCCGCCGCTTTGTACGGAAACTCCACAACACTATTACAAGGCTTATTAGCCGGCGGAGGCAGCGAGCAACAGCAAGTCTCAGGAGCGGGTATGAACTTCCCATACAATACCCATTTCGGAATGAACTCCAACGAGTTAATGGCGGCAGCATCATGGTCGAACTCCAAAGTACCTCCGTTCTTAAGAAACTCACCACCAAAAGGAGCAGCACCGACGCCAGCACAGAGTCAGTTACAGTTTTCTAACAACACAGCATTCTGGAACGCGTCAGACATGAAAGACGCGAGGGCGAGTTATATTCCGGCGTCGTACAACGCTGCAGCCTTGGCGGGAGATAAATCAAAGGTAAAAACAATAAAAAGCAATTGGTTGGGTTATGATTAGGGTTGTGAATAATGATGATTGAAATTGAAATTGGTAACAGAGTAGATCAGAGGGTGGAAAGAAAAGTGGGAATGATCAGAATCAACAACAAACGGGTGGCGGCGCCGGCGGTGGTGGTGTTGGTGGTGCGGTTAAAAGGCCTCGAAATGAAACGGCGCCGGGATTGCCAGCGTTTAAGGTCACTGAACTGAATTGAGTTATAGAGAGATTAGTAAATTGGGAAAAAAGAGGAAAACAGAAGAGATGAAAATGGGGTTGGGTTTTGCAGGTGAGGAAAGAGAAGATGGGGGACAGAATCACTGCGCTCCAACAACTGGTCTCACCTTTCGGAAAGGTAACATCATTACTCAAATTAAAATTAATTCAAAATTTATGATAATGAATGATATGAAATGAATGTTTGTGATTTTGTGCCACCAGACTGATACCGCTTCAGTGCTGTCTGAAGCCATTGAATACATAAAGTTCCTCCATGAACAAGTCAGTGTAAGTGCTCACTTAATGTCTTTTTTTGTTTTAAATTTGGAAAAACATTATAGTTATTTTAAAAAAAAAAAAAAAAAAAAAAATTCCAGGTATTGAGCACTCCATATTTGAAGAGCGGTGGTGCAGTACAGCAGCAGCAGCAGCAGAAATGGTGTGAGAAAAGGAAGGAAGGAGAAGGAAAAGAAGAGGATCTAAGAAGCAGAGGGCTTTGTTTAGTTCCAGTTTCAAGTACATTTCCGGTGACCCACGAAACCACCGTCGATTTCTGGACTCCAAGCTTCGGAGGAACCTTCCGATAA

mRNA sequence

TTTCTTCTGATTTTAATTTTGTTTATTTTATTTTAGTTGCAGTGCAAGCAATATAAATAGCGATATGCCGCGAAGCGTTTTCAATCGTTTTTTTTTGTAAATAGAAATTCCACACACGAACACAGAACAAATCCGAAAGAACCAAATCCCAAATCATCAAACTGGGTTTGAGATTCAGAGCTGCAGTAGCCATGGCGGAGGAGTTTCAGAGCACTGGAAACTGGTGGGACGCTTCTAGAAGCCGCTACGAAGCAGGAATATCCCCTTCTTCCTCCGCCATAACTACTTTCGTCGACCACCCGGACTCCGGCGCCGGCGCAGGCGACCCCAATTTGCACATCATGGGCTTGGGCCTCGATTGGAACCAACCCCTCTTTCGGGGCGGCGGAGAGAAGGCGGCGGAGAGCAGTTTCCGGTCGATGCTGCAGCCGGATAATATGAATTTGAATATGCAAGAAACAGGGCAACACCACCAACACCAACAACAACAGATTCAATGGATGAGATCGGAGAAGCTATATTCAGGGGAATCTCCAGCGACTGAATTCAAGCCAATAAACAGAGGGTTTTCACTAGATCACCACAACCAGCCTCCGCCACAGTTCAGCCCTTCTCATTACAGCACCGGCGACAGCACCATCACCAGTTTTCCAATCGACACCGCCGCCGCCGCTTTGTACGGAAACTCCACAACACTATTACAAGGCTTATTAGCCGGCGGAGGCAGCGAGCAACAGCAAGTCTCAGGAGCGGGTATGAACTTCCCATACAATACCCATTTCGGAATGAACTCCAACGAGTTAATGGCGGCAGCATCATGGTCGAACTCCAAAGTACCTCCGTTCTTAAGAAACTCACCACCAAAAGGAGCAGCACCGACGCCAGCACAGAGTCAGTTACAGTTTTCTAACAACACAGCATTCTGGAACGCGTCAGACATGAAAGACGCGAGGGCGAGTTATATTCCGGCGTCGTACAACGCTGCAGCCTTGGCGGGAGATAAATCAAAGAGTAGATCAGAGGGTGGAAAGAAAAGTGGGAATGATCAGAATCAACAACAAACGGGTGGCGGCGCCGGCGGTGGTGGTGTTGGTGGTGCGGTTAAAAGGCCTCGAAATGAAACGGCGCCGGGATTGCCAGCGTTTAAGGTGAGGAAAGAGAAGATGGGGGACAGAATCACTGCGCTCCAACAACTGGTCTCACCTTTCGGAAAGACTGATACCGCTTCAGTGCTGTCTGAAGCCATTGAATACATAAAGTTCCTCCATGAACAAGTCAGTGTATTGAGCACTCCATATTTGAAGAGCGGTGGTGCAGTACAGCAGCAGCAGCAGCAGAAATGGTGTGAGAAAAGGAAGGAAGGAGAAGGAAAAGAAGAGGATCTAAGAAGCAGAGGGCTTTGTTTAGTTCCAGTTTCAAGTACATTTCCGGTGACCCACGAAACCACCGTCGATTTCTGGACTCCAAGCTTCGGAGGAACCTTCCGATAA

Coding sequence (CDS)

ATGGCGGAGGAGTTTCAGAGCACTGGAAACTGGTGGGACGCTTCTAGAAGCCGCTACGAAGCAGGAATATCCCCTTCTTCCTCCGCCATAACTACTTTCGTCGACCACCCGGACTCCGGCGCCGGCGCAGGCGACCCCAATTTGCACATCATGGGCTTGGGCCTCGATTGGAACCAACCCCTCTTTCGGGGCGGCGGAGAGAAGGCGGCGGAGAGCAGTTTCCGGTCGATGCTGCAGCCGGATAATATGAATTTGAATATGCAAGAAACAGGGCAACACCACCAACACCAACAACAACAGATTCAATGGATGAGATCGGAGAAGCTATATTCAGGGGAATCTCCAGCGACTGAATTCAAGCCAATAAACAGAGGGTTTTCACTAGATCACCACAACCAGCCTCCGCCACAGTTCAGCCCTTCTCATTACAGCACCGGCGACAGCACCATCACCAGTTTTCCAATCGACACCGCCGCCGCCGCTTTGTACGGAAACTCCACAACACTATTACAAGGCTTATTAGCCGGCGGAGGCAGCGAGCAACAGCAAGTCTCAGGAGCGGGTATGAACTTCCCATACAATACCCATTTCGGAATGAACTCCAACGAGTTAATGGCGGCAGCATCATGGTCGAACTCCAAAGTACCTCCGTTCTTAAGAAACTCACCACCAAAAGGAGCAGCACCGACGCCAGCACAGAGTCAGTTACAGTTTTCTAACAACACAGCATTCTGGAACGCGTCAGACATGAAAGACGCGAGGGCGAGTTATATTCCGGCGTCGTACAACGCTGCAGCCTTGGCGGGAGATAAATCAAAGAGTAGATCAGAGGGTGGAAAGAAAAGTGGGAATGATCAGAATCAACAACAAACGGGTGGCGGCGCCGGCGGTGGTGGTGTTGGTGGTGCGGTTAAAAGGCCTCGAAATGAAACGGCGCCGGGATTGCCAGCGTTTAAGGTGAGGAAAGAGAAGATGGGGGACAGAATCACTGCGCTCCAACAACTGGTCTCACCTTTCGGAAAGACTGATACCGCTTCAGTGCTGTCTGAAGCCATTGAATACATAAAGTTCCTCCATGAACAAGTCAGTGTATTGAGCACTCCATATTTGAAGAGCGGTGGTGCAGTACAGCAGCAGCAGCAGCAGAAATGGTGTGAGAAAAGGAAGGAAGGAGAAGGAAAAGAAGAGGATCTAAGAAGCAGAGGGCTTTGTTTAGTTCCAGTTTCAAGTACATTTCCGGTGACCCACGAAACCACCGTCGATTTCTGGACTCCAAGCTTCGGAGGAACCTTCCGATAA

Protein sequence

MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQPLFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFKPINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTVDFWTPSFGGTFR
Homology
BLAST of Cp4.1LG01g08430 vs. ExPASy Swiss-Prot
Match: Q8GXT3 (Transcription factor bHLH123 OS=Arabidopsis thaliana OX=3702 GN=BHLH123 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.4e-59
Identity = 197/477 (41.30%), Postives = 245/477 (51.36%), Query Frame = 0

Query: 4   EFQSTGNWWDASRSRYEAGISP------SSSAITTFVDHPDSGAGAGDPNLHIMGLGL-- 63
           +F ++G+WW  S S   +  S        S     F D     + A D +L ++GLGL  
Sbjct: 6   DFINSGSWWKVSSSSSPSSSSSMRASSIESGGSAVFHDKLHHHSLATDHHLQMIGLGLSS 65

Query: 64  -----DWNQPLFRGGGEKAAESSFRSMLQPDNMNL------NMQETGQHHQHQQQQIQWM 123
                 WNQ L R  G+  AE+SF  MLQ +N+NL      N   T     +Q Q+    
Sbjct: 66  QSPVDQWNQSLLR--GDSKAETSFGVMLQ-ENLNLDATSNANANTTSSTSSYQLQESDSS 125

Query: 124 RSEKLYSGESPATEFKPI------NRGFSLDHHNQPPPQFSPSHYSTGDS---TITSFPI 183
              +      P ++FKP       NRGF LDH      QFSP   S+ DS   T   F +
Sbjct: 126 HHHQAL-WRDPQSDFKPQILTSGGNRGFFLDH------QFSPHGSSSTDSSTVTCQGFAV 185

Query: 184 DTAAAALYGNSTTLLQGLLAGGGSEQQQVSGAGMN--FPYNTHFGMNSNELMAAASWSN- 243
           D ++ A+Y  +TT      + G    QQ  G G +   P   H   +       +S  N 
Sbjct: 186 DNSSNAMYAATTTTPNS--SSGMFHHQQAGGFGSSDQQPSRNHQQSSLGYSQFGSSTGNY 245

Query: 244 ----SKVPP--FLRNSPPKGAAPTPAQSQLQFSNNTAFWN--------ASDMKDARASYI 303
               S +P   FLR+SPP    P P  S L+FSNN  FWN        A    DA +++ 
Sbjct: 246 DQMASALPSTWFLRSSPP----PKP-HSPLRFSNNATFWNPAAAGNAGAPPPHDASSNFF 305

Query: 304 PASYNA---AALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGVGGAVKRPRNETAPGL 363
           PA            ++ K+ SE    S N+  +       GG     A KR ++E A   
Sbjct: 306 PALQPPQIHPQSFDEQPKNISEIRDSSSNEVKR-------GGNDHQPAAKRAKSEAASPS 365

Query: 364 PAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSGGA 423
           PAFK RKEKMGDRI ALQQLVSPFGKTD ASVLSEAIEYIKFLH+QVS LS PY+KSG +
Sbjct: 366 PAFK-RKEKMGDRIAALQQLVSPFGKTDAASVLSEAIEYIKFLHQQVSALSNPYMKSGAS 425

Query: 424 VQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTVDFWTPSFGGTFR 433
           +Q QQ     E       +E DLRSRGLCLVPVSSTFPVTH+TTVDFWTP+FGGTFR
Sbjct: 426 LQHQQSDHSTELE---VSEEPDLRSRGLCLVPVSSTFPVTHDTTVDFWTPTFGGTFR 454

BLAST of Cp4.1LG01g08430 vs. ExPASy Swiss-Prot
Match: Q94JL3 (Transcription factor bHLH112 OS=Arabidopsis thaliana OX=3702 GN=BHLH112 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.4e-38
Identity = 107/249 (42.97%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 190 NFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSNNTAFWNASD 249
           NF   T   +N  +L    SW+N       + +P    A     S    +N+  FWN+S 
Sbjct: 168 NFVSTTSGSINDPQL----SWAN-------KTNPHHQVAYGLINSFSNNANSRPFWNSSS 227

Query: 250 MKDAR----ASYIPASYNAAALAGDKSK-----SRSEGGKKSGNDQNQQQTGGGAGGGGV 309
             +      ++++      +    DK+K     ++SE  K++ ++++             
Sbjct: 228 TTNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNES------------- 287

Query: 310 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 369
             A K+PR  T   LP FKVRKE + D+IT+LQQLVSPFGKTDTASVL EAIEYIKFLH+
Sbjct: 288 --AAKKPRVTTPSPLPTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHD 347

Query: 370 QVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTV 429
           QV+VLSTPY+K  GA  QQQQQ   + + + E +  +LR  GLCLVP+SSTFPV +ETT 
Sbjct: 348 QVTVLSTPYMKQ-GASNQQQQQISGKSKSQDENENHELRGHGLCLVPISSTFPVANETTA 389

BLAST of Cp4.1LG01g08430 vs. ExPASy Swiss-Prot
Match: Q9M0X8 (Transcription factor bHLH114 OS=Arabidopsis thaliana OX=3702 GN=BHLH114 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 2.3e-30
Identity = 74/119 (62.18%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 304 VKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVS 363
           +KRPR ET   LP+FKVRKEK+GDRITALQQLVSPFGKTDTASVL+EA+EYIKFL EQV+
Sbjct: 158 LKRPRLETLSPLPSFKVRKEKLGDRITALQQLVSPFGKTDTASVLNEAVEYIKFLQEQVT 217

Query: 364 VLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEE--------DLRSRGLCLVPVSSTFPV 415
           VLS P   + G+VQQQQ         +GE +E+        DL SRGLCL+P+S+++PV
Sbjct: 218 VLSNPEQNTIGSVQQQQCSNKKSINTQGEVEEDECSPRRYVDLSSRGLCLMPISASYPV 276

BLAST of Cp4.1LG01g08430 vs. ExPASy Swiss-Prot
Match: Q8VZ22 (Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.2e-28
Identity = 73/129 (56.59%), Postives = 91/129 (70.54%), Query Frame = 0

Query: 304 VKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVS 363
           +KRPR ET    P+FKVRKEK+GDRITALQQLVSPFGKTDTASVL +AI+YIKFL EQ++
Sbjct: 175 LKRPRLETPSHFPSFKVRKEKLGDRITALQQLVSPFGKTDTASVLHDAIDYIKFLQEQIT 234

Query: 364 --VLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKE-----EDLRSRGLCLVPVSSTF--PV 423
             V ++P+L S G+ +Q+Q   W +K       +     +DLRSRGLCL+P+SSTF  P 
Sbjct: 235 EKVSTSPHLNSIGSGEQKQ---WSDKSSNNTHNQNCSPRQDLRSRGLCLMPISSTFSTPP 294

BLAST of Cp4.1LG01g08430 vs. ExPASy Swiss-Prot
Match: Q9SFZ3 (Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.3e-25
Identity = 70/136 (51.47%), Postives = 84/136 (61.76%), Query Frame = 0

Query: 303 AVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQV 362
           A K+PR E+    P FKVRKEK+GDRI ALQQLVSPFGKTDTASVL EAI YIKFL  Q+
Sbjct: 316 ASKKPRVESRSSCPPFKVRKEKLGDRIAALQQLVSPFGKTDTASVLMEAIGYIKFLQSQI 375

Query: 363 SVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHE----- 422
             LS PY+++      +  Q   + ++  E +  DLRSRGLCLVP+S    VT +     
Sbjct: 376 ETLSVPYMRASRNRPGKASQLVSQSQEGDEEETRDLRSRGLCLVPLSCMTYVTGDGGDGG 435

Query: 423 --TTVDFW--TPSFGG 430
                 FW   P FGG
Sbjct: 436 GGVGTGFWPTPPGFGG 451

BLAST of Cp4.1LG01g08430 vs. NCBI nr
Match: XP_023546795.1 (transcription factor bHLH123-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857 bits (2213), Expect = 1.96e-313
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSE 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSE
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSE 180

Query: 181 QQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSN 240
           QQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSN
Sbjct: 181 QQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSN 240

Query: 241 NTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGV 300
           NTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGV
Sbjct: 241 NTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGV 300

Query: 301 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360
           GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE
Sbjct: 301 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360

Query: 361 QVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTV 420
           QVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTV
Sbjct: 361 QVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTV 420

Query: 421 DFWTPSFGGTFR 432
           DFWTPSFGGTFR
Sbjct: 421 DFWTPSFGGTFR 432

BLAST of Cp4.1LG01g08430 vs. NCBI nr
Match: KAG6600752.1 (Transcription factor basic helix-loop-helix 123, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 820 bits (2117), Expect = 8.06e-299
Identity = 419/436 (96.10%), Postives = 423/436 (97.02%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALA DKSKSRSEGGKKSGNDQNQQQTGGG GG 
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALASDKSKSRSEGGKKSGNDQNQQQTGGGGGG- 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
               AVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 ----AVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGGA--VQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTH 420
           HEQVSVLSTPYLKSGG   VQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTH
Sbjct: 361 HEQVSVLSTPYLKSGGGAVVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTH 420

Query: 421 ETTVDFWTPSFGGTFR 432
           ETTVDFWTPSFGGTFR
Sbjct: 421 ETTVDFWTPSFGGTFR 431

BLAST of Cp4.1LG01g08430 vs. NCBI nr
Match: KAG7031392.1 (Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 819 bits (2116), Expect = 1.38e-298
Identity = 422/438 (96.35%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALA DKSKSRSEGGKKSGNDQNQQQTGGG GGG
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALASDKSKSRSEGGKKSGNDQNQQQTGGGGGGG 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
           G  GAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 G--GAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGG-AVQQQQQ---QKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPV 420
           HEQVSVLSTPYLKSGG AV QQQQ   QKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPV
Sbjct: 361 HEQVSVLSTPYLKSGGGAVVQQQQHHHQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPV 420

Query: 421 THETTVDFWTPSFGGTFR 432
           THETTVDFWTPSFGGTFR
Sbjct: 421 THETTVDFWTPSFGGTFR 436

BLAST of Cp4.1LG01g08430 vs. NCBI nr
Match: XP_022941615.1 (transcription factor bHLH123-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 818 bits (2114), Expect = 2.14e-298
Identity = 419/435 (96.32%), Postives = 423/435 (97.24%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG    
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG---- 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
             GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 --GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420
           HEQVSVLSTPYLKSGG AV QQQQQKWCEKR EGEGKEEDLRSRGLCLVPVSSTFPVTHE
Sbjct: 361 HEQVSVLSTPYLKSGGGAVVQQQQQKWCEKRTEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420

Query: 421 TTVDFWTPSFGGTFR 432
           TTVDFWTPSFGGTFR
Sbjct: 421 TTVDFWTPSFGGTFR 429

BLAST of Cp4.1LG01g08430 vs. NCBI nr
Match: XP_022941616.1 (transcription factor bHLH123-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 810 bits (2093), Expect = 3.15e-295
Identity = 417/435 (95.86%), Postives = 421/435 (96.78%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG    
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG---- 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
             GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 --GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420
           HEQV  LSTPYLKSGG AV QQQQQKWCEKR EGEGKEEDLRSRGLCLVPVSSTFPVTHE
Sbjct: 361 HEQV--LSTPYLKSGGGAVVQQQQQKWCEKRTEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420

Query: 421 TTVDFWTPSFGGTFR 432
           TTVDFWTPSFGGTFR
Sbjct: 421 TTVDFWTPSFGGTFR 427

BLAST of Cp4.1LG01g08430 vs. ExPASy TrEMBL
Match: A0A6J1FU65 (transcription factor bHLH123-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446921 PE=4 SV=1)

HSP 1 Score: 818 bits (2114), Expect = 1.04e-298
Identity = 419/435 (96.32%), Postives = 423/435 (97.24%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG    
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG---- 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
             GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 --GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420
           HEQVSVLSTPYLKSGG AV QQQQQKWCEKR EGEGKEEDLRSRGLCLVPVSSTFPVTHE
Sbjct: 361 HEQVSVLSTPYLKSGGGAVVQQQQQKWCEKRTEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420

Query: 421 TTVDFWTPSFGGTFR 432
           TTVDFWTPSFGGTFR
Sbjct: 421 TTVDFWTPSFGGTFR 429

BLAST of Cp4.1LG01g08430 vs. ExPASy TrEMBL
Match: A0A6J1FLL0 (transcription factor bHLH123-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446921 PE=4 SV=1)

HSP 1 Score: 810 bits (2093), Expect = 1.52e-295
Identity = 417/435 (95.86%), Postives = 421/435 (96.78%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWWDA+RSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWDATRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAA--LYGNSTTLLQGLLAGGG 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFP+DTAAAA  LYGNSTTLLQGLLAGGG
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPLDTAAAAAALYGNSTTLLQGLLAGGG 180

Query: 181 SEQQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQF 240
           SEQQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNSKVPPFLRNSPPKGAAPTP QSQLQF
Sbjct: 181 SEQQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSKVPPFLRNSPPKGAAPTPPQSQLQF 240

Query: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGG 300
           SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG    
Sbjct: 241 SNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGG---- 300

Query: 301 GVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360
             GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL
Sbjct: 301 --GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFL 360

Query: 361 HEQVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420
           HEQV  LSTPYLKSGG AV QQQQQKWCEKR EGEGKEEDLRSRGLCLVPVSSTFPVTHE
Sbjct: 361 HEQV--LSTPYLKSGGGAVVQQQQQKWCEKRTEGEGKEEDLRSRGLCLVPVSSTFPVTHE 420

Query: 421 TTVDFWTPSFGGTFR 432
           TTVDFWTPSFGGTFR
Sbjct: 421 TTVDFWTPSFGGTFR 427

BLAST of Cp4.1LG01g08430 vs. ExPASy TrEMBL
Match: A0A6J1JW46 (transcription factor bHLH123-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488889 PE=4 SV=1)

HSP 1 Score: 806 bits (2081), Expect = 8.83e-294
Identity = 409/433 (94.46%), Postives = 415/433 (95.84%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPD+GAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDAGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEK AESSFRSMLQPDNMNLNMQETGQHHQH+QQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKGAESSFRSMLQPDNMNLNMQETGQHHQHEQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSE 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDST+TSFPID+AAAALY NSTTLLQGLLAGGGSE
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTVTSFPIDSAAAALYVNSTTLLQGLLAGGGSE 180

Query: 181 QQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSN 240
           QQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNS VPPFLRNSPPKGAAPTPAQSQLQFSN
Sbjct: 181 QQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSTVPPFLRNSPPKGAAPTPAQSQLQFSN 240

Query: 241 NTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGV 300
           NTAFWNASDMKDARASY+PASYN AALAGDKSKS SEGGKKSGNDQNQQQTGGG      
Sbjct: 241 NTAFWNASDMKDARASYVPASYNGAALAGDKSKSTSEGGKKSGNDQNQQQTGGG------ 300

Query: 301 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360
               KRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE
Sbjct: 301 ----KRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360

Query: 361 QVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT 420
           QVSVLSTPYLKSGG AV QQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT
Sbjct: 361 QVSVLSTPYLKSGGGAVVQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT 420

Query: 421 VDFWTPSFGGTFR 432
           VDFWTP FGGTFR
Sbjct: 421 VDFWTPGFGGTFR 423

BLAST of Cp4.1LG01g08430 vs. ExPASy TrEMBL
Match: A0A6J1JQB5 (transcription factor bHLH123-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488889 PE=4 SV=1)

HSP 1 Score: 798 bits (2060), Expect = 1.30e-290
Identity = 407/433 (94.00%), Postives = 413/433 (95.38%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPD+GAGAGDPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDAGAGAGDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQQIQWMRSEKLYSGESPATEFK 120
           LFRGGGEK AESSFRSMLQPDNMNLNMQETGQHHQH+QQQIQWMRSEKLYSGESPATEFK
Sbjct: 61  LFRGGGEKGAESSFRSMLQPDNMNLNMQETGQHHQHEQQQIQWMRSEKLYSGESPATEFK 120

Query: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTITSFPIDTAAAALYGNSTTLLQGLLAGGGSE 180
           PINRGFSLDHHNQPPPQFSPSHYSTGDST+TSFPID+AAAALY NSTTLLQGLLAGGGSE
Sbjct: 121 PINRGFSLDHHNQPPPQFSPSHYSTGDSTVTSFPIDSAAAALYVNSTTLLQGLLAGGGSE 180

Query: 181 QQQVSGAGMNFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSN 240
           QQQVSGAGMNFPYNTHFGMNS+ELMAAASWSNS VPPFLRNSPPKGAAPTPAQSQLQFSN
Sbjct: 181 QQQVSGAGMNFPYNTHFGMNSSELMAAASWSNSTVPPFLRNSPPKGAAPTPAQSQLQFSN 240

Query: 241 NTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGV 300
           NTAFWNASDMKDARASY+PASYN AALAGDKSKS SEGGKKSGNDQNQQQTGGG      
Sbjct: 241 NTAFWNASDMKDARASYVPASYNGAALAGDKSKSTSEGGKKSGNDQNQQQTGGG------ 300

Query: 301 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360
               KRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE
Sbjct: 301 ----KRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 360

Query: 361 QVSVLSTPYLKSGG-AVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT 420
           QV  LSTPYLKSGG AV QQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT
Sbjct: 361 QV--LSTPYLKSGGGAVVQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETT 420

Query: 421 VDFWTPSFGGTFR 432
           VDFWTP FGGTFR
Sbjct: 421 VDFWTPGFGGTFR 421

BLAST of Cp4.1LG01g08430 vs. ExPASy TrEMBL
Match: A0A0A0L564 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G088430 PE=4 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 2.31e-214
Identity = 338/455 (74.29%), Postives = 359/455 (78.90%), Query Frame = 0

Query: 1   MAEEFQSTGNWWDASRSRYEAGISPSSSAITTFVDHPDSGAGAGDPNLHIMGLGLDWNQP 60
           MAEEFQS+GNWW+A+     + ISPSSS+ITTFVDH DS A A DPNLHIMGLGLDWNQP
Sbjct: 1   MAEEFQSSGNWWEAA-----SRISPSSSSITTFVDHSDSAAAASDPNLHIMGLGLDWNQP 60

Query: 61  LFRGGGEKAAESSFRSMLQPDNMNLNMQETGQHHQHQQQ-----QIQWMRSEKLYSGESP 120
           LFRGGGEKAAE SFRSMLQPDNMNLNM+ETGQ  Q QQQ     QIQWMRSEKLYSGESP
Sbjct: 61  LFRGGGEKAAEGSFRSMLQPDNMNLNMEETGQQQQQQQQEQQQQQIQWMRSEKLYSGESP 120

Query: 121 ATEFKPINRGFSLDHHN-------QPPPQFS-PSHYSTGDSTITSFPIDTAAAALYGNST 180
           AT+FKPINRGFSLDHH+       Q  PQFS PSHYS+GDS +TS+PIDT A  LYGNS 
Sbjct: 121 ATDFKPINRGFSLDHHHHHHHHHHQAQPQFSSPSHYSSGDSAVTSYPIDTNAN-LYGNSA 180

Query: 181 TLLQGLLAGGGSEQQQVS---GAGMNFPYNTHFGMNSNELMAA-ASWSNSKVPPFLRNSP 240
           TLLQGLLA GG +QQQ       GMNFPYN+HFGMNS ELM   ASWS SKVPP+LRNSP
Sbjct: 181 TLLQGLLAAGGEQQQQQQQQISMGMNFPYNSHFGMNSGELMTGGASWSPSKVPPYLRNSP 240

Query: 241 PKGAAPTPAQSQLQFSNNTAFWNASDMKDARASYIPASYNAAALAGDKSKSRSEGG---- 300
           PK  A     SQLQFSNNTAFWNASDMK+ R SY   SYNAAA   +KSK+ SE G    
Sbjct: 241 PKAGAGGNPHSQLQFSNNTAFWNASDMKEVRPSYFAPSYNAAAGFTEKSKNISEVGDSVT 300

Query: 301 -KKSGNDQNQQQTGGGAGGGGVGGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSP 360
            KKSGND NQQ             A KRPRNET   LPAFKVRKEKMGDRITALQQLVSP
Sbjct: 301 TKKSGNDNNQQSA-----------AAKRPRNETPSPLPAFKVRKEKMGDRITALQQLVSP 360

Query: 361 FGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSGGAVQQQQQQKWCEKR-KEGEGKEED 420
           FGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSG  VQQQ QQ+  EK  KEGEG ++D
Sbjct: 361 FGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSGAVVQQQHQQQRNEKSVKEGEGGKQD 420

Query: 421 LRSRGLCLVPVSSTFPVTHETTVDFWTPSFGGTFR 432
           LRSRGLCLVPVSSTFPVTHETTVDFWTP+FGGTFR
Sbjct: 421 LRSRGLCLVPVSSTFPVTHETTVDFWTPTFGGTFR 438

BLAST of Cp4.1LG01g08430 vs. TAIR 10
Match: AT3G20640.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-60
Identity = 197/477 (41.30%), Postives = 245/477 (51.36%), Query Frame = 0

Query: 4   EFQSTGNWWDASRSRYEAGISP------SSSAITTFVDHPDSGAGAGDPNLHIMGLGL-- 63
           +F ++G+WW  S S   +  S        S     F D     + A D +L ++GLGL  
Sbjct: 6   DFINSGSWWKVSSSSSPSSSSSMRASSIESGGSAVFHDKLHHHSLATDHHLQMIGLGLSS 65

Query: 64  -----DWNQPLFRGGGEKAAESSFRSMLQPDNMNL------NMQETGQHHQHQQQQIQWM 123
                 WNQ L R  G+  AE+SF  MLQ +N+NL      N   T     +Q Q+    
Sbjct: 66  QSPVDQWNQSLLR--GDSKAETSFGVMLQ-ENLNLDATSNANANTTSSTSSYQLQESDSS 125

Query: 124 RSEKLYSGESPATEFKPI------NRGFSLDHHNQPPPQFSPSHYSTGDS---TITSFPI 183
              +      P ++FKP       NRGF LDH      QFSP   S+ DS   T   F +
Sbjct: 126 HHHQAL-WRDPQSDFKPQILTSGGNRGFFLDH------QFSPHGSSSTDSSTVTCQGFAV 185

Query: 184 DTAAAALYGNSTTLLQGLLAGGGSEQQQVSGAGMN--FPYNTHFGMNSNELMAAASWSN- 243
           D ++ A+Y  +TT      + G    QQ  G G +   P   H   +       +S  N 
Sbjct: 186 DNSSNAMYAATTTTPNS--SSGMFHHQQAGGFGSSDQQPSRNHQQSSLGYSQFGSSTGNY 245

Query: 244 ----SKVPP--FLRNSPPKGAAPTPAQSQLQFSNNTAFWN--------ASDMKDARASYI 303
               S +P   FLR+SPP    P P  S L+FSNN  FWN        A    DA +++ 
Sbjct: 246 DQMASALPSTWFLRSSPP----PKP-HSPLRFSNNATFWNPAAAGNAGAPPPHDASSNFF 305

Query: 304 PASYNA---AALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGVGGAVKRPRNETAPGL 363
           PA            ++ K+ SE    S N+  +       GG     A KR ++E A   
Sbjct: 306 PALQPPQIHPQSFDEQPKNISEIRDSSSNEVKR-------GGNDHQPAAKRAKSEAASPS 365

Query: 364 PAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVSVLSTPYLKSGGA 423
           PAFK RKEKMGDRI ALQQLVSPFGKTD ASVLSEAIEYIKFLH+QVS LS PY+KSG +
Sbjct: 366 PAFK-RKEKMGDRIAALQQLVSPFGKTDAASVLSEAIEYIKFLHQQVSALSNPYMKSGAS 425

Query: 424 VQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTVDFWTPSFGGTFR 433
           +Q QQ     E       +E DLRSRGLCLVPVSSTFPVTH+TTVDFWTP+FGGTFR
Sbjct: 426 LQHQQSDHSTELE---VSEEPDLRSRGLCLVPVSSTFPVTHDTTVDFWTPTFGGTFR 454

BLAST of Cp4.1LG01g08430 vs. TAIR 10
Match: AT1G61660.3 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-40
Identity = 106/244 (43.44%), Postives = 140/244 (57.38%), Query Frame = 0

Query: 190 NFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSNNTAFWNASD 249
           NF   T   +N  +L    SW+N       + +P    A     S    +N+  FWN+S 
Sbjct: 168 NFVSTTSGSINDPQL----SWAN-------KTNPHHQVAYGLINSFSNNANSRPFWNSSS 227

Query: 250 MKDAR----ASYIPASYNAAALAGDKSKSRSEGGKKSGNDQNQQQTGGGAGGGGVGGAVK 309
             +      ++++      +    DK+K+R++         N+              A K
Sbjct: 228 TTNLNNTTPSNFVTTPQIISTRLEDKTKTRAQSESLKRAKDNE-------------SAAK 287

Query: 310 RPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVSVL 369
           +PR  T   LP FKVRKE + D+IT+LQQLVSPFGKTDTASVL EAIEYIKFLH+QV+VL
Sbjct: 288 KPRVTTPSPLPTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHDQVTVL 347

Query: 370 STPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTVDFWTP 429
           STPY+K  GA  QQQQQ   + + + E +  +LR  GLCLVP+SSTFPV +ETT DFWTP
Sbjct: 348 STPYMKQ-GASNQQQQQISGKSKSQDENENHELRGHGLCLVPISSTFPVANETTADFWTP 386

BLAST of Cp4.1LG01g08430 vs. TAIR 10
Match: AT1G61660.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 9.6e-40
Identity = 107/249 (42.97%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 190 NFPYNTHFGMNSNELMAAASWSNSKVPPFLRNSPPKGAAPTPAQSQLQFSNNTAFWNASD 249
           NF   T   +N  +L    SW+N       + +P    A     S    +N+  FWN+S 
Sbjct: 168 NFVSTTSGSINDPQL----SWAN-------KTNPHHQVAYGLINSFSNNANSRPFWNSSS 227

Query: 250 MKDAR----ASYIPASYNAAALAGDKSK-----SRSEGGKKSGNDQNQQQTGGGAGGGGV 309
             +      ++++      +    DK+K     ++SE  K++ ++++             
Sbjct: 228 TTNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNES------------- 287

Query: 310 GGAVKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHE 369
             A K+PR  T   LP FKVRKE + D+IT+LQQLVSPFGKTDTASVL EAIEYIKFLH+
Sbjct: 288 --AAKKPRVTTPSPLPTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHD 347

Query: 370 QVSVLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEEDLRSRGLCLVPVSSTFPVTHETTV 429
           QV+VLSTPY+K  GA  QQQQQ   + + + E +  +LR  GLCLVP+SSTFPV +ETT 
Sbjct: 348 QVTVLSTPYMKQ-GASNQQQQQISGKSKSQDENENHELRGHGLCLVPISSTFPVANETTA 389

BLAST of Cp4.1LG01g08430 vs. TAIR 10
Match: AT4G05170.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.6e-31
Identity = 74/119 (62.18%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 304 VKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVS 363
           +KRPR ET   LP+FKVRKEK+GDRITALQQLVSPFGKTDTASVL+EA+EYIKFL EQV+
Sbjct: 98  LKRPRLETLSPLPSFKVRKEKLGDRITALQQLVSPFGKTDTASVLNEAVEYIKFLQEQVT 157

Query: 364 VLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKEE--------DLRSRGLCLVPVSSTFPV 415
           VLS P   + G+VQQQQ         +GE +E+        DL SRGLCL+P+S+++PV
Sbjct: 158 VLSNPEQNTIGSVQQQQCSNKKSINTQGEVEEDECSPRRYVDLSSRGLCLMPISASYPV 216

BLAST of Cp4.1LG01g08430 vs. TAIR 10
Match: AT4G21340.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-29
Identity = 73/129 (56.59%), Postives = 91/129 (70.54%), Query Frame = 0

Query: 304 VKRPRNETAPGLPAFKVRKEKMGDRITALQQLVSPFGKTDTASVLSEAIEYIKFLHEQVS 363
           +KRPR ET    P+FKVRKEK+GDRITALQQLVSPFGKTDTASVL +AI+YIKFL EQ++
Sbjct: 175 LKRPRLETPSHFPSFKVRKEKLGDRITALQQLVSPFGKTDTASVLHDAIDYIKFLQEQIT 234

Query: 364 --VLSTPYLKSGGAVQQQQQQKWCEKRKEGEGKE-----EDLRSRGLCLVPVSSTF--PV 423
             V ++P+L S G+ +Q+Q   W +K       +     +DLRSRGLCL+P+SSTF  P 
Sbjct: 235 EKVSTSPHLNSIGSGEQKQ---WSDKSSNNTHNQNCSPRQDLRSRGLCLMPISSTFSTPP 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXT32.4e-5941.30Transcription factor bHLH123 OS=Arabidopsis thaliana OX=3702 GN=BHLH123 PE=1 SV=... [more]
Q94JL31.4e-3842.97Transcription factor bHLH112 OS=Arabidopsis thaliana OX=3702 GN=BHLH112 PE=1 SV=... [more]
Q9M0X82.3e-3062.18Transcription factor bHLH114 OS=Arabidopsis thaliana OX=3702 GN=BHLH114 PE=2 SV=... [more]
Q8VZ222.2e-2856.59Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=... [more]
Q9SFZ31.3e-2551.47Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_023546795.11.96e-313100.00transcription factor bHLH123-like [Cucurbita pepo subsp. pepo][more]
KAG6600752.18.06e-29996.10Transcription factor basic helix-loop-helix 123, partial [Cucurbita argyrosperma... [more]
KAG7031392.11.38e-29896.35Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022941615.12.14e-29896.32transcription factor bHLH123-like isoform X1 [Cucurbita moschata][more]
XP_022941616.13.15e-29595.86transcription factor bHLH123-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1FU651.04e-29896.32transcription factor bHLH123-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1FLL01.52e-29595.86transcription factor bHLH123-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JW468.83e-29494.46transcription factor bHLH123-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1JQB51.30e-29094.00transcription factor bHLH123-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0L5642.31e-21474.29BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G088430 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G20640.11.7e-6041.30basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G61660.31.9e-4043.44basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G61660.19.6e-4042.97basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G05170.11.6e-3162.18basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G21340.11.5e-2956.59basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 320..364
e-value: 0.0037
score: 26.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 309..358
score: 12.614082
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 319..387
e-value: 9.0E-8
score: 34.1
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 319..371
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..319
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..143
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..235
NoneNo IPR availablePANTHERPTHR16223TRANSCRIPTION FACTOR BHLH83-RELATEDcoord: 44..422
NoneNo IPR availablePANTHERPTHR16223:SF46TRANSCRIPTION FACTOR BHLH123coord: 44..422
NoneNo IPR availableCDDcd11393bHLH_AtbHLH_likecoord: 315..366
e-value: 2.28036E-24
score: 93.0147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g08430.1Cp4.1LG01g08430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding