Cp4.1LG01g08290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g08290
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6-like
LocationCp4.1LG01: 4990193 .. 4992279 (-)
RNA-Seq ExpressionCp4.1LG01g08290
SyntenyCp4.1LG01g08290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTTGTTTATGTGTTGTCTATAAATAAGAGAATTGTTCCATTGTTTTCCCCCTCCCACGCATTCCTTCCCTCCTCCCTCTCTCTAACAAACTCCCATTCTCTCCAAAAACAAAAAATGCCTGAAGCTCCAAACGCACCCTCTCTGCTGGAAAAAAACAGCGCCGCCCTCCAATTCATCGAAGACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCCCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATTGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTAACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGACCCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTATTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAAAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACACTTAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTCATTGAGGGGGAGTGCTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGGCTTCCCTTGGTGTGTAGCATGTATGCTTCTTCCGAGTGCTATTTTGGCCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTAGTCACAACTTATGCTGGTAAAACCATTCTATTCGACTATTGTTTATGGAAGCCTATGAAAATGAAATTATTTTGTTTCAATTCAACAGGATTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCGGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAACGTGGTTCTAAGCATTGACTCCGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCATCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCAGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCGGAGTGCCGCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAGACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGATAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAGGCAGATTTTTATGCGCTTTTTCTTTTTGTTCTTTTTTTCCCCCTTGCAATCAAATAATATGTATGTTCTTGTGATGATCATGTTAAAAGCATCATCAATTCACAA

mRNA sequence

ATGAGCTTAATTGTTCCATTGTTTTCCCCCTCCCACGCATTCCTTCCCTCCTCCCTCTCTCTAACAAACTCCCATTCTCTCCAAAAACAAAAAATGCCTGAAGCTCCAAACGCACCCTCTCTGCTGGAAAAAAACAGCGCCGCCCTCCAATTCATCGAAGACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCCCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATTGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTAACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGACCCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTATTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAAAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACACTTAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTCATTGAGGGGGAGTGCTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGGCTTCCCTTGGTGTGTAGCATGTATGCTTCTTCCGAGTGCTATTTTGGCCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTAGTCACAACTTATGCTGGATTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCGGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAACGTGGTTCTAAGCATTGACTCCGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCATCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCAGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCGGAGTGCCGCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAGACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGATAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAGGCAGATTTTTATGCGCTTTTTCTTTTTGTTCTTTTTTTCCCCCTTGCAATCAAATAATATGTATGTTCTTGTGATGATCATGTTAAAAGCATCATCAATTCACAA

Coding sequence (CDS)

ATGAGCTTAATTGTTCCATTGTTTTCCCCCTCCCACGCATTCCTTCCCTCCTCCCTCTCTCTAACAAACTCCCATTCTCTCCAAAAACAAAAAATGCCTGAAGCTCCAAACGCACCCTCTCTGCTGGAAAAAAACAGCGCCGCCCTCCAATTCATCGAAGACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCCCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATTGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTAACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGACCCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTATTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAAAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACACTTAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTCATTGAGGGGGAGTGCTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGGCTTCCCTTGGTGTGTAGCATGTATGCTTCTTCCGAGTGCTATTTTGGCCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTAGTCACAACTTATGCTGGATTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCGGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAACGTGGTTCTAAGCATTGACTCCGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCATCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCAGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCGGAGTGCCGCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAGACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGATAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAG

Protein sequence

MSLIVPLFSPSHAFLPSSLSLTNSHSLQKQKMPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN
Homology
BLAST of Cp4.1LG01g08290 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 5.2e-273
Identity = 457/611 (74.80%), Postives = 520/611 (85.11%), Query Frame = 0

Query: 32  MPEAP-------NAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLH- 91
           MPEAP       +  SL EKN   LQFIED+TTNA  VQR+VL +ILSRNA  EYL  H 
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 92  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 151
              R   +TFK  +P+++Y+ IQP I RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 152 PTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 211
           PTI EELDRRSLLYSLLMPVM QF+PGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 212 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 271
           SSHFK+RPYDPYTNYTSPN+ ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 272 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 331
           +FLEKHW  L  DIR+GTL+S+ITD +VREAV    + +PDP+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 332 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 391
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 392 AYTLIPSMAYFEFLPLQTN--------------HNHEHQLVDLTDVELGKEYELVVTTYA 451
           +YTLIP+MAYFEFLP+  N                 + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 452 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFD 511
           GLYRYRVGD+L VAGFKNNAPQF+F+CRKNVVLSIDSDKTDEVELQNAVKN++ HLVPFD
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 512 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSD 571
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCLT+EESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 572 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPK 619
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVVDSYFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Cp4.1LG01g08290 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 8.5e-268
Identity = 451/611 (73.81%), Postives = 515/611 (84.29%), Query Frame = 0

Query: 32  MPEAPNAPSLL-------EKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLH- 91
           MPEAP   SL        +KN   LQ IE++T+NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 92  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 151
              R   +TFK+ +P+I+Y+ I+P I RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 152 PTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 211
           PTI EELDRRSLLYSLLMPVM+QF+PGLE GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 212 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 271
           SSHFK+RPYDPYTNYTSPNE ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 272 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 331
           +FLEKHW  L  DIR+GTL+S ITD +VREAV  + + +P P+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 332 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 391
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 392 AYTLIPSMAYFEFLPLQTNH--------------NHEHQLVDLTDVELGKEYELVVTTYA 451
           +YTLIPSMAYFEFLP+  N+                + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 452 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFD 511
           GL RYRVGD+LRV GFKN APQF+F+CRKNVVLSIDSDKTDEVELQNAVKN++ HLVPFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 512 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSD 571
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 572 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPK 619
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVVDSYFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Cp4.1LG01g08290 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.1e-238
Identity = 404/601 (67.22%), Postives = 481/601 (80.03%), Query Frame = 0

Query: 32  MPEAPNAPSLLE------KNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHAR 91
           MPEAP A +          ++ AL+FIE +T NA  VQR+VL +IL++NA +EYL  +  
Sbjct: 1   MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGI 60

Query: 92  P-SP---DTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 151
           P SP   D F+  +PL++Y+ +QP I RIANGDTSPI    PISEFLTSSGTSGGERKLM
Sbjct: 61  PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLM 120

Query: 152 PTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 211
           PTI +E++RRSLLYSLLMPVM+Q + GL+KGK MY LFVKAE+RTPGGL ARPVLTSYY+
Sbjct: 121 PTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYR 180

Query: 212 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 271
           S  F DRP DPYT+YTSP+EAILC DSYQSMYAQ+LCGL HR DVLRVGAVFASGF+RAI
Sbjct: 181 SRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAI 240

Query: 272 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 331
            FLEKHW  LCHDIR+G L+ +ITD+ VR+AV    + R DP L D IE EC + SW+GI
Sbjct: 241 HFLEKHWARLCHDIRTGELDPEITDRVVRDAV--GRVLRADPALADAIEDECARASWEGI 300

Query: 332 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 391
           I RLWP TKY+DVIVTG MSQYI TL++Y  GLPL C+MYASSECYFGLNLNP+C P++V
Sbjct: 301 IRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDV 360

Query: 392 AYTLIPSMAYFEFLPLQTNH----NHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 451
           AYTLIP+M Y+EFLP+  N+         LVDL DV+LG EYELVVTTY+GLYRYRVGD+
Sbjct: 361 AYTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDV 420

Query: 452 LRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYA 511
           LRVAGFKN AP F+FV R+NV LS+DSDKTDE EL  AV  ++ HL PF A+LV+YTSYA
Sbjct: 421 LRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYA 480

Query: 512 NTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 571
           + +TIPGHYV++WE+ + GST VP SVFE+CCL++EE+LNSVYRQGR  D+SIGPLEI+V
Sbjct: 481 DAATIPGHYVLFWEL-RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRV 540

Query: 572 VEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQ 619
           V  GTFDKLMDYAI+ GASINQYK PRCV+  P+V+LL++RV   YFSPKCPKW  G+KQ
Sbjct: 541 VAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQ 598

BLAST of Cp4.1LG01g08290 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.9e-228
Identity = 394/601 (65.56%), Postives = 474/601 (78.87%), Query Frame = 0

Query: 33  PEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-----S 92
           P A  A ++  ++   L+ IE +T NA   QR+VL +IL++NA +EYL     P     +
Sbjct: 11  PAAAAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGA 70

Query: 93  PDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEE 152
            + F+   PL++Y+ I P + RIANGDTSPIL   P+SEFLTSSGTSGGERKLMPTI EE
Sbjct: 71  DEAFRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEE 130

Query: 153 LDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKD 212
           ++RRS LYSLLMPVM++ +PGL+KGK MY  FVK+E RTPGGL ARPVLTS+Y+S +F +
Sbjct: 131 MERRSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLE 190

Query: 213 RPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKH 272
           RP+DPYT YTSP+EA+LC D+YQSMYAQ++CGL HR DVLRVGAVFASGF+RAIRFLEKH
Sbjct: 191 RPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKH 250

Query: 273 WQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWP 332
           W  LC DIR+G L+  +TD AVR AV   VLR  DP L D IE EC + SWQGII R+WP
Sbjct: 251 WPSLCRDIRAGELDGGVTDPAVRGAV-GRVLRGADPALADAIEAECARPSWQGIIRRVWP 310

Query: 333 NTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIP 392
           +TKY+DVIVTGAM+QYI TL++Y  GLPL C+MYASSECYFGLNLNP+C P+EVAYTLIP
Sbjct: 311 STKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIP 370

Query: 393 SMAYFEFLPLQTNHNH------EHQ-LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 452
           +M YFEFLP+ +  N       +H+ LVDL DV+LG EYELVVTTY+GLYRYRVGD+LRV
Sbjct: 371 TMCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRV 430

Query: 453 AGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANT- 512
           AGFKN AP F FV RKNV LSIDSDKTDE EL  AV  ++ HL PF A+LV+YTSYA+T 
Sbjct: 431 AGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTA 490

Query: 513 STIPGHYVVYWEV-NQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVV 572
           +TIPGHYV++WE+ +  G T VP SVFEDCCL +EE LNSVYRQ R +D+SIGPLEI+VV
Sbjct: 491 TTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVV 550

Query: 573 EIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVA-GHKQ 619
             GTFDKLMDYA++ GASINQYK PRCV+  P+V+LL+ RV  +YFSPKCPKW A G+KQ
Sbjct: 551 ADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQ 610

BLAST of Cp4.1LG01g08290 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 763.8 bits (1971), Expect = 1.5e-219
Identity = 375/589 (63.67%), Postives = 454/589 (77.08%), Query Frame = 0

Query: 36  PNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLT---LHARPSPDTFK 95
           P A ++ E +   L+FI+++TTN   VQ +VL +IL RNA +EYLT   L        F+
Sbjct: 17  PAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFR 76

Query: 96  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 155
           + +P++SYD +QP+I RIANGD SPIL + P+SEFLTSSGTS GERKLMPTI +ELDRR 
Sbjct: 77  AKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQ 136

Query: 156 LLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 215
           LLYSLLMPVM  ++PGL+KGKG+YFLFVK+E +TPGGL ARPVLTSYYKS HFK+RPYDP
Sbjct: 137 LLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDP 196

Query: 216 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 275
           Y NYTSP  AILC D++QSMYAQ++CGLC R DVLR+GAVFASG +RAIRFL+ +W+ L 
Sbjct: 197 YHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLA 256

Query: 276 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 335
            DI SG L  ++TD +VREAV   +L  PDPEL   I  EC KG W GIITR+WPNTKY+
Sbjct: 257 DDIESGELTPRVTDPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYL 316

Query: 336 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 395
           DVIVTGAM+QYI TL++YS GLP+ C+MYASSECYFGLNL P+C P+EV+YT++P+M YF
Sbjct: 317 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYF 376

Query: 396 EFLPLQTN---HNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQ 455
           EFLP+           QLVDL  VE+G+EYELV+TTYAGL RYRVGD+LRV GF N APQ
Sbjct: 377 EFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQ 436

Query: 456 FNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVY 515
           F FV RKNV+LSI+SDKTDE ELQ AV+ +   L P  A++V+YTS A T  IPGHYV+Y
Sbjct: 437 FRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIY 496

Query: 516 WEVNQK--GSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLM 575
           WE+  K  G+T V       CCL MEE+LN+VYRQ RV+D SIGPLEI+VV  GTF++LM
Sbjct: 497 WELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELM 556

Query: 576 DYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHK 617
           DYAI+ GASINQYK PRCV F PIV+LL+SRVV S+FSP  P W    +
Sbjct: 557 DYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Cp4.1LG01g08290 vs. NCBI nr
Match: XP_023511762.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1219 bits (3155), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. NCBI nr
Match: KAG6600750.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1214 bits (3140), Expect = 0.0
Identity = 594/596 (99.66%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. NCBI nr
Match: XP_022942727.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1211 bits (3133), Expect = 0.0
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN APQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. NCBI nr
Match: XP_022985796.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1207 bits (3124), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. NCBI nr
Match: XP_008452341.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA0047007.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa] >TYK04773.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 521/598 (87.12%), Postives = 561/598 (93.81%), Query Frame = 0

Query: 33  PEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 92
           P  PN   L++KN A LQFIED+TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 93  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 152
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 153 LLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 212
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 213 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 272
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 273 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 332
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 333 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 392
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 393 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 452
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 453 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHY 512
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 513 VVYWEVNQK--GSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 572
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 573 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQD 622
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVV SYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

BLAST of Cp4.1LG01g08290 vs. ExPASy TrEMBL
Match: A0A6J1FR29 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447677 PE=3 SV=1)

HSP 1 Score: 1211 bits (3133), Expect = 0.0
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN APQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. ExPASy TrEMBL
Match: A0A6J1J981 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483735 PE=3 SV=1)

HSP 1 Score: 1207 bits (3124), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 32  MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 91
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 92  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 151
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 152 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 211
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 212 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 271
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 272 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 331
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 332 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 391
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 392 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 451
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 452 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 511
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 512 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 571
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 572 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 627
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

BLAST of Cp4.1LG01g08290 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 521/598 (87.12%), Postives = 561/598 (93.81%), Query Frame = 0

Query: 33  PEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 92
           P  PN   L++KN A LQFIED+TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 93  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 152
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 153 LLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 212
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 213 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 272
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 273 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 332
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 333 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 392
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 393 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 452
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 453 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHY 512
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 513 VVYWEVNQK--GSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 572
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 573 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQD 622
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVV SYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

BLAST of Cp4.1LG01g08290 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 521/598 (87.12%), Postives = 561/598 (93.81%), Query Frame = 0

Query: 33  PEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 92
           P  PN   L++KN A LQFIED+TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 93  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 152
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 153 LLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 212
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 213 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 272
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 273 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 332
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 333 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 392
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 393 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 452
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 453 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHY 512
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 513 VVYWEVNQK--GSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 572
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 573 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQD 622
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVV SYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

BLAST of Cp4.1LG01g08290 vs. ExPASy TrEMBL
Match: A0A6J1EW65 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111438458 PE=3 SV=1)

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 521/605 (86.12%), Postives = 563/605 (93.06%), Query Frame = 0

Query: 32  MPEAPNAPS-------LLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHA 91
           MPEAP  P+       L++KN+A LQFIED+TTNA  VQRQVLSQILSRNA+SEYL+LHA
Sbjct: 1   MPEAPKTPTSVTPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLHA 60

Query: 92  RPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI 151
           RPSPDTFKSSIPL+SYDHIQPFI+RIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI
Sbjct: 61  RPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI 120

Query: 152 HEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH 211
            EEL+RRSLLYSLLMP+MTQF+PGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH
Sbjct: 121 EEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH 180

Query: 212 FKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFL 271
           FK+RPYDPYTNYTSPNEAILC D+YQS+YAQ+LCGLC+RLDVLRVGAVFASGFIRAIRFL
Sbjct: 181 FKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRFL 240

Query: 272 EKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITR 331
           EKHWQLLCHDIR+GTLNSQITDQAV +AV++ VLRRPDPEL DFI  +C KG W+GIITR
Sbjct: 241 EKHWQLLCHDIRTGTLNSQITDQAVGDAVML-VLRRPDPELADFIHEQCSKGYWKGIITR 300

Query: 332 LWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYT 391
           LWPNTKY+DVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYT
Sbjct: 301 LWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYT 360

Query: 392 LIPSMAYFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 451
           LIPSMAYFEFLP+Q   N+     + QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 LIPSMAYFEFLPVQRTSNNGESLNDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 452 VAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANT 511
           VAGFKN APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTS+ANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSHANT 480

Query: 512 STIPGHYVVYWEVNQKGS--TGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 571
           STIPGHYV+YWE+NQKGS  T VPPSV EDCCLT+EESLNSVYRQGRV+DKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWELNQKGSSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEIKV 540

Query: 572 VEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQ 622
           VE GTFDKLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVV  YFSPKCPKWVAGHKQ
Sbjct: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGHKQ 600

BLAST of Cp4.1LG01g08290 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-274
Identity = 457/611 (74.80%), Postives = 520/611 (85.11%), Query Frame = 0

Query: 32  MPEAP-------NAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLH- 91
           MPEAP       +  SL EKN   LQFIED+TTNA  VQR+VL +ILSRNA  EYL  H 
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 92  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 151
              R   +TFK  +P+++Y+ IQP I RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 152 PTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 211
           PTI EELDRRSLLYSLLMPVM QF+PGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 212 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 271
           SSHFK+RPYDPYTNYTSPN+ ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 272 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 331
           +FLEKHW  L  DIR+GTL+S+ITD +VREAV    + +PDP+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 332 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 391
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 392 AYTLIPSMAYFEFLPLQTN--------------HNHEHQLVDLTDVELGKEYELVVTTYA 451
           +YTLIP+MAYFEFLP+  N                 + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 452 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFD 511
           GLYRYRVGD+L VAGFKNNAPQF+F+CRKNVVLSIDSDKTDEVELQNAVKN++ HLVPFD
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 512 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSD 571
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCLT+EESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 572 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPK 619
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVVDSYFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Cp4.1LG01g08290 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 924.1 bits (2387), Expect = 6.1e-269
Identity = 451/611 (73.81%), Postives = 515/611 (84.29%), Query Frame = 0

Query: 32  MPEAPNAPSLL-------EKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLH- 91
           MPEAP   SL        +KN   LQ IE++T+NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 92  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 151
              R   +TFK+ +P+I+Y+ I+P I RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 152 PTIHEELDRRSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 211
           PTI EELDRRSLLYSLLMPVM+QF+PGLE GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 212 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 271
           SSHFK+RPYDPYTNYTSPNE ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 272 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 331
           +FLEKHW  L  DIR+GTL+S ITD +VREAV  + + +P P+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 332 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 391
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 392 AYTLIPSMAYFEFLPLQTNH--------------NHEHQLVDLTDVELGKEYELVVTTYA 451
           +YTLIPSMAYFEFLP+  N+                + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 452 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFD 511
           GL RYRVGD+LRV GFKN APQF+F+CRKNVVLSIDSDKTDEVELQNAVKN++ HLVPFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 512 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSD 571
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 572 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPK 619
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVVDSYFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Cp4.1LG01g08290 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 763.5 bits (1970), Expect = 1.4e-220
Identity = 379/582 (65.12%), Postives = 456/582 (78.35%), Query Frame = 0

Query: 35  APNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYL---TLHARPSPDTF 94
           +P  P   EK++ AL+FIE++T NA  VQ  +L++IL+RNA +EYL    L      DTF
Sbjct: 9   SPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTF 68

Query: 95  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 154
           K+ IP+I+Y+ +QP I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI EELDRR
Sbjct: 69  KTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRR 128

Query: 155 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 214
            LLYSLLMPVM  ++PGL+KGKGMYFLFVK+E +TPGGL ARPVLTSYYKS HF+ RPYD
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYD 188

Query: 215 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 274
           PY  YTSPNEAILC DS+QSMY Q+LCGL  RL VLRVGAVFASG +RAIRFL+ HW   
Sbjct: 189 PYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRF 248

Query: 275 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 334
            HDI  G L+S+ITD ++R+   MS + +PDP L +FI  EC   +W+ IITR+WPNTKY
Sbjct: 249 AHDIELGCLDSEITDPSIRQC--MSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKY 308

Query: 335 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 394
           +DVIVTGAM+QYI TL+YYS GLP+ C+MYASSECYFGLNLNP+  P+EV+YT++P+MAY
Sbjct: 309 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAY 368

Query: 395 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 454
           FEF+PL        + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N+APQF+
Sbjct: 369 FEFIPLGGT-----KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFH 428

Query: 455 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHL-VPFDATLVDYTSYANTSTIPGHYVVYW 514
           FV RKNV+LSIDSDKTDE ELQ AV+N+ + L     + + +YTSYA+TSTIPGHYV+YW
Sbjct: 429 FVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYW 488

Query: 515 EVNQKGSTGVPP-SVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDY 574
           E+  +     P       CCL MEESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDY
Sbjct: 489 ELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDY 548

Query: 575 AITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKW 612
           AI+ GASINQYK PRCV F PIV+LL+SRVV ++FSP  P W
Sbjct: 549 AISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

BLAST of Cp4.1LG01g08290 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 748.4 bits (1931), Expect = 4.6e-216
Identity = 365/585 (62.39%), Postives = 456/585 (77.95%), Query Frame = 0

Query: 43  EKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYL---TLHARPSPDTFKSSIPLIS 102
           EK+  AL+FIE++T N   VQ +VL +IL+RN+++EYL    L       TFKS +P+++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 103 YDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSLLM 162
           Y+ ++P I RI+NGD SPIL S PI+EFLTSSGTS GERKLMPTI E+LDRR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 163 PVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSP 222
           PVM  ++PGL+KGKG+YFLFVK+E++T GGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 223 NEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRSGT 282
           NEAILC+DS QSMYAQ+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L  DI +GT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 283 LNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVTGA 342
           L+S+I D A++   +  +L +PD EL +F+ G C + +W+GIIT++WPNTKY+DVIVTGA
Sbjct: 258 LSSRIFDPAIKNR-MSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGA 317

Query: 343 MSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPLQT 402
           M+QYI TL+YYS GLP+ C+MYASSE YFG+NL P+C P+EV+YT++P+MAYFEFLP   
Sbjct: 318 MAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--- 377

Query: 403 NHNH-----------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAP 462
            HNH           E  LV+L +VE+GKEYELV+TTYAGLYRYRVGDILRV GF N+AP
Sbjct: 378 -HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAP 437

Query: 463 QFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVV 522
           QF F+ RKNV+LS++SDKTDE ELQ AV+N+          +++YTSYA T TIPGHYV+
Sbjct: 438 QFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVI 497

Query: 523 YWEV--NQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 582
           YWE+    + +  +   V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV  GTF++L
Sbjct: 498 YWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEEL 557

Query: 583 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKW 612
           MDYAI+ GASINQYK PRCV F PI++LL+SRVV ++FSP  P W
Sbjct: 558 MDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 597

BLAST of Cp4.1LG01g08290 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 734.9 bits (1896), Expect = 5.2e-212
Identity = 365/582 (62.71%), Postives = 452/582 (77.66%), Query Frame = 0

Query: 35  APNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLT---LHARPSPDTF 94
           +P   S   K+  AL+FIE++T N   VQ++V+ +ILSRN+ +EYL    L       TF
Sbjct: 9   SPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTF 68

Query: 95  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 154
           K+ +P++ YD ++P I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI E++DRR
Sbjct: 69  KTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRR 128

Query: 155 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 214
            LLYSLLMPVM  ++PGL+KGK +YFLFVK E++TPGGL ARPVLTSYYKS  FK RP D
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPND 188

Query: 215 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 274
           PY  YTSPNEAILC DS QSMY Q+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L
Sbjct: 189 PYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKEL 248

Query: 275 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCK-GSWQGIITRLWPNTK 334
             DI +GTL+S+I+D A++E+ +  +L +PD EL DFI   C +  SW+GIIT++WPNTK
Sbjct: 249 ADDISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTK 308

Query: 335 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 394
           Y+DVIVTGAM+QYI  L+YYS GLP+ C+MYASSE YFG+NL P+C P+EV+YT++P+MA
Sbjct: 309 YLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMA 368

Query: 395 YFEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQF 454
           YFEFLP       + +LV+L DVE+GKEYELV+TTYAGL RYRVGDIL+V GF N+APQF
Sbjct: 369 YFEFLPHHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQF 428

Query: 455 NFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYW 514
            FV RKNV+LSI+SDKTDE ELQ+AV+N+   L      +++YTSYA T TIPGHYV+YW
Sbjct: 429 KFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYW 488

Query: 515 EVNQKGSTGVP-PSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDY 574
           E+  K  T  P   V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV+ GTF++LMDY
Sbjct: 489 ELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDY 548

Query: 575 AITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKW 612
           AI+ GASINQYK PRCV F PI++LL+SRVV ++FSP  P W
Sbjct: 549 AISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LSQ45.2e-27374.80Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818298.5e-26873.81Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM54.1e-23867.22Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ63.9e-22865.56Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
A3BLS01.5e-21963.67Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Match NameE-valueIdentityDescription
XP_023511762.10.0100.00indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
KAG6600750.10.099.66Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022942727.10.099.33indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_022985796.10.098.99indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_008452341.10.087.12PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA004700... [more]
Match NameE-valueIdentityDescription
A0A6J1FR290.099.33indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1J9810.098.99indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A5D3BYD30.087.12Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BTN30.087.12indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493... [more]
A0A6J1EW650.086.12indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT5G54510.13.7e-27474.80Auxin-responsive GH3 family protein [more]
AT4G27260.16.1e-26973.81Auxin-responsive GH3 family protein [more]
AT2G14960.11.4e-22065.12Auxin-responsive GH3 family protein [more]
AT4G37390.14.6e-21662.39Auxin-responsive GH3 family protein [more]
AT2G23170.15.2e-21262.71Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 52..588
e-value: 5.4E-197
score: 655.8
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 35..619
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 35..619

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g08290.1Cp4.1LG01g08290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity