Cp4.1LG01g06690 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g06690 vs. ExPASy Swiss-Prot
Match: Q1PF50 (Probable serine protease EDA2 OS=Arabidopsis thaliana OX=3702 GN=EDA2 PE=2 SV=2)

HSP 1 Score: 603.6 bits (1555), Expect = 2.2e-171
Identity = 294/514 (57.20%), Postives = 366/514 (71.21%), Query Frame = 0

Query: 14  VAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYF 73
           V+ +  S A ++       +S    ++T  E WF+QTLDH S  +  KF+QRY+EF+DYF
Sbjct: 17  VSFSTLSHALLHPSSVSHNVSRSRYYMTTNELWFNQTLDHESPNDHRKFRQRYYEFMDYF 76

Query: 74  RIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRY 133
           R PDGP+F  ICGEGPC+GIANDY+++LAKKF A +VSLEHRYYGKSSPF SL T NL+Y
Sbjct: 77  RSPDGPMFMIICGEGPCSGIANDYINVLAKKFQAGVVSLEHRYYGKSSPFNSLATENLKY 136

Query: 134 LSSKQALFDLAVFRQYYQDSLNLKLDRK---SENPWFVFGVSYSGALSAWFQLKFPHLTC 193
           LSSKQAL+DLA FRQYYQ+SLN KL+     S+NPWF FG+SYSGALSAWF+LKFPHLTC
Sbjct: 137 LSSKQALYDLASFRQYYQESLNKKLNISSGGSDNPWFFFGISYSGALSAWFRLKFPHLTC 196

Query: 194 GSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESA 253
           GSLASSAVV A++ F++FDQQ                                  IGESA
Sbjct: 197 GSLASSAVVRAIYEFSEFDQQ----------------------------------IGESA 256

Query: 254 GPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDK 313
           G  CK ALQETN+L+E    +  + V++LF A E++VD DF Y  ADAA MAFQYGNPDK
Sbjct: 257 GQECKLALQETNKLLELGLKVKNKAVKSLFNATELDVDADFLYLTADAAVMAFQYGNPDK 316

Query: 314 LCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQ 373
           LC PLVEAK  G DLV  Y+ YV+ Y +  +G  VRTY +KHL+NT +  DSA RLWWFQ
Sbjct: 317 LCVPLVEAKKNGSDLVVTYSTYVREYCMRIWGLRVRTYNRKHLRNTVVTADSAYRLWWFQ 376

Query: 374 VCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIY 433
            CTE+ YFQVAP  DS+RS ++           N  +HLDLCK++FG+  YP VD TN+Y
Sbjct: 377 ACTELGYFQVAPKYDSVRSHQI-----------NTTFHLDLCKSLFGKDVYPKVDATNLY 436

Query: 434 YGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIE 493
           YGG ++A +KI+FTNGS+DPWRHASKQ  + +MPSY++ CRNCGHG+D+RGCPQSP  IE
Sbjct: 437 YGGDRLAATKIIFTNGSEDPWRHASKQNSTHEMPSYIIKCRNCGHGSDIRGCPQSPMVIE 485

Query: 494 GDAHSCTSPATVYKVRQQLVKKMDLWLSECQASI 525
           G +++C+ P  V KVRQQ+V+ +DLWLSEC+ SI
Sbjct: 497 GKSNNCSLPDYVNKVRQQMVEHIDLWLSECRQSI 485

BLAST of Cp4.1LG01g06690 vs. ExPASy Swiss-Prot
Match: Q9QXE5 (Thymus-specific serine protease OS=Mus musculus OX=10090 GN=Prss16 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.2e-34
Identity = 128/422 (30.33%), Postives = 188/422 (44.55%), Query Frame = 0

Query: 46  WFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPC--NGIANDYLSILAK 105
           W +Q LD F+  +R  F QRY+    +    D P+F  I GEG      +   + + LA 
Sbjct: 60  WLEQPLDPFNASDRRTFLQRYWVNDQHRTGQDVPVFLHIGGEGSLGPGSVMAGHPAALAP 119

Query: 106 KFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSE 165
            +GA ++SLEHR+YG S P   L    LRYLSS+ AL D+A  RQ     LN+     S 
Sbjct: 120 AWGALVISLEHRFYGLSMPAGGLDLALLRYLSSRHALADVASARQALSGLLNV----SSS 179

Query: 166 NPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQV----IQISCYW 225
           +PW  FG SY+G+L+ W +LKFPHL   ++ASSA + AV +F+ ++Q V     Q++   
Sbjct: 180 SPWICFGGSYAGSLATWARLKFPHLVFAAVASSAPLSAVVDFSAYNQVVARSLTQVAIGG 239

Query: 226 LYAFLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQAL 285
               L + S  F    R+            AGPA +A L+E  +L         E+   L
Sbjct: 240 SLECLAAASTAFTEVERLL----------RAGPAAQAVLRE--ELGACGSLDLTEDQAEL 299

Query: 286 FGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAY--AKYVKNYF 345
            GA +  V G   Y     A ++       +  C L+  K   +     Y   +      
Sbjct: 300 LGALQALVGGTVQYDGQAGAPLSV------RQLCGLLLGKWGNRSRSTPYLGLRRAVQIV 359

Query: 346 LGSFGSNVRTYIQ----KHLKNTTLGEDS-ADRLWWFQVCTEVAYFQVAPANDSIRSSKV 405
           L S G    ++ +      L NT        DR W +Q CTE  ++      + ++    
Sbjct: 360 LRSMGQKCLSFSRAETVAQLSNTEPQVSGVGDRQWLYQTCTEFGFYVTC---EGLQCPFS 419

Query: 406 DISYLFTNNSSNHRYHLDLCKNVFG---EGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQD 452
            +  L         + L+LC+ VFG         V  TN YYGG     ++++F NG  D
Sbjct: 420 QLPAL--------PFQLELCEQVFGLSPASVAQAVAQTNSYYGGQSPGATQVLFVNGDTD 448

BLAST of Cp4.1LG01g06690 vs. ExPASy Swiss-Prot
Match: Q9NQE7 (Thymus-specific serine protease OS=Homo sapiens OX=9606 GN=PRSS16 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 1.2e-33
Identity = 143/492 (29.07%), Postives = 213/492 (43.29%), Query Frame = 0

Query: 46  WFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPC--NGIANDYLSILAK 105
           W +Q LD F+  +R  F QRY+    ++   DGPIF  + GEG      +   + + LA 
Sbjct: 61  WLEQLLDPFNVSDRRSFLQRYWVNDQHWVGQDGPIFLHLGGEGSLGPGSVMRGHPAALAP 120

Query: 106 KFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSE 165
            +GA ++SLEHR+YG S P   L    LR+LSS+ AL D+   R       N+     S 
Sbjct: 121 AWGALVISLEHRFYGLSIPAGGLEMAQLRFLSSRLALADVVSARLALSRLFNI----SSS 180

Query: 166 NPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAF 225
           +PW  FG SY+G+L+AW +LKFPHL   S+ASSA V AV +F++++  V +     L + 
Sbjct: 181 SPWICFGGSYAGSLAAWARLKFPHLIFASVASSAPVRAVLDFSEYNDVVSRS----LMST 240

Query: 226 LLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQA-LFGA 285
            +  S+  R  A ++  F        +G A +AAL+     +     + + E QA L GA
Sbjct: 241 AIGGSLECR--AAVSVAFAEVERRLRSGGAAQAALRTE---LSACGPLGRAENQAELLGA 300

Query: 286 EEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYA--KYVKNYFLGS 345
            +  V G   Y     A ++       +  C L+      +     Y   +      L S
Sbjct: 301 LQALVGGVVQYDGQTGAPLSV------RQLCGLLLGGGGNRSHSTPYCGLRRAVQIVLHS 360

Query: 346 FG------SNVRTYIQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI 405
            G      S   T  Q       L     DR W +Q CTE  ++       +  + +   
Sbjct: 361 LGQKCLSFSRAETVAQLRSTEPQL-SGVGDRQWLYQTCTEFGFYV------TCENPRCPF 420

Query: 406 SYLFTNNSSNHRYHLDLCKNVFG---EGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDPW 465
           S L    S      LDLC+ VFG         V  TN YYGG     +K++F NG  DPW
Sbjct: 421 SQLPALPS-----QLDLCEQVFGLSALSVAQAVAQTNSYYGGQTPGANKVLFVNGDTDPW 480

Query: 466 RHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLVK 524
            H      +       +  R   H  D+   P+ P +         SP ++   RQ + +
Sbjct: 481 -HVLSVTQALGSSESTLLIRTGSHCLDM--APERPSD---------SP-SLRLGRQNIFQ 508

BLAST of Cp4.1LG01g06690 vs. ExPASy Swiss-Prot
Match: P34528 (Putative serine protease K12H4.7 OS=Caenorhabditis elegans OX=6239 GN=K12H4.7 PE=3 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 8.0e-33
Identity = 138/489 (28.22%), Postives = 211/489 (43.15%), Query Frame = 0

Query: 47  FDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDY----LSILA 106
           F QTLDHF       FQQRY+    +++   GP F  + GEGP +     Y    ++ LA
Sbjct: 63  FTQTLDHFDSSVGKTFQQRYYHNNQWYK-AGGPAFLMLGGEGPESSYWVSYPGLEITNLA 122

Query: 107 KKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKS 166
            K GA +  +EHR+YG++ P   ++  NL+YLSS QA+ D A F +    ++  K  + +
Sbjct: 123 AKQGAWVFDIEHRFYGETHPTSDMSVPNLKYLSSAQAIEDAAAFIK----AMTAKFPQLA 182

Query: 167 ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYA 226
              W  FG SYSGAL+AW + K P L   ++ SS  V A  +F ++  +V+Q S      
Sbjct: 183 NAKWVTFGGSYSGALAAWTRAKHPELVYAAVGSSGPVQAEVDFKEY-LEVVQNS------ 242

Query: 227 FLLSDSINFRFYARITFFFFFFXIGESAG-PACKAA--LQETNQLVEQRFAINKEEVQAL 286
            +  +S          F      +  S G    K A  L +  Q+ ++      E V + 
Sbjct: 243 -ITRNSTECAASVTQGFNLVASLLQTSDGRKQLKTAFHLCQDIQMDDKSLKYFWETVYSP 302

Query: 287 FGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYF-- 346
           +  E ++  GD       A + A Q      +C   +  K+     +    K V +YF  
Sbjct: 303 Y-MEVVQYSGD------AAGSFATQLTISHAICRYHINTKSTPLQKL----KQVNDYFNQ 362

Query: 347 -LGSFGSNVRTY--IQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI 406
             G FG N   Y      +K+ T GE  +DR W +Q CTE  Y+Q         SS    
Sbjct: 363 VSGYFGCNDIDYNGFISFMKDETFGEAQSDRAWVWQTCTEFGYYQST-------SSATAG 422

Query: 407 SYLFTNNSSNHRYHLDLCKNVFGEGF-----YPDVDTTNIYYGG-TKIAGSKIVFTNGSQ 466
            +    ++   +Y++D C  ++G  +        VD TN YYGG   +   +I+  NG  
Sbjct: 423 PWFGGVSNLPAQYYIDECTAIYGAAYNSQEVQTSVDYTNQYYGGRDNLNTDRILLPNGDI 482

Query: 467 DPWRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQ 518
           DPW HA  ++ S +     V      H  D+ G             S      +   RQ+
Sbjct: 483 DPW-HALGKLTSSNSNIVPVVINGTAHCADMYGA------------SSLDSMYLTNARQR 507

BLAST of Cp4.1LG01g06690 vs. ExPASy Swiss-Prot
Match: P90893 (Putative serine protease F56F10.1 OS=Caenorhabditis elegans OX=6239 GN=F56F10.1 PE=1 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 2.4e-29
Identity = 136/473 (28.75%), Postives = 203/473 (42.92%), Query Frame = 0

Query: 47  FDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNG--IANDYLSIL--A 106
           F Q LDHF  YN   + Q+YF +   F   +  IF  I GEGP NG   AN  +  L  A
Sbjct: 59  FTQKLDHFDPYNTKTWNQKYF-YNPVFSRNNSIIFLMIGGEGPENGKWAANPNVQYLQWA 118

Query: 107 KKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKS 166
           K+FGA +  LEHR++G S P   + T++LRYL+++QAL DLA F ++       K  R  
Sbjct: 119 KEFGADVFDLEHRFFGDSWPIPDMQTSSLRYLTTQQALADLAFFIEFMNQQYGFKNPR-- 178

Query: 167 ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYA 226
              W  FG SY G+L+AWF+ K+P LT GS+ASSA V    +F +             YA
Sbjct: 179 ---WVTFGGSYPGSLAAWFRQKYPQLTVGSVASSAPVNLKLDFYE-------------YA 238

Query: 227 FLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQL---VEQRFAINKE-EVQA 286
            ++ D +      RIT              A K A  +  +L    E R ++N    +Q 
Sbjct: 239 MVVEDDL------RIT--------DPKCAQATKDAFVQMQKLALTAEGRNSLNNHFNLQP 298

Query: 287 LFGAEEMEVD-GDFFYFLADAAAMAFQY---GNPD---------KLCCPLVEAKNAGKDL 346
            F A   ++D  +FF  + +      QY   G  +         K+C  +  A     D+
Sbjct: 299 PFDANTTKLDINNFFGNIFNTYQGMTQYTYDGQSNSTHSDKTVRKMCDIMTNATET--DV 358

Query: 347 VDAYAKYVKNYFLGSFGSNVRTYIQKHLKNT--------------TLGED-SADRLWWFQ 406
           V      V+N FL        +     + N+               LG D +A R W + 
Sbjct: 359 V----MRVENLFLWFNQMEPASANLTVMPNSYWDVISQVGSGDLNVLGPDGAAARGWMWL 418

Query: 407 VCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGFY------PDV 466
            C E+ + Q     +++  + V ++ LF          +D+C ++FG+          + 
Sbjct: 419 CCNEIGFLQTTNQGNNVFGTGVPLN-LF----------IDMCTDMFGDSMKMSQIMGGNK 478

Query: 467 DTTNIYYGGTKIAGSKIVFTNGSQDPWRHASK--QIPSPDMPSYLVT-CRNCG 475
            + N Y G      + +V  NGS DPW        I S  +  YL+    +CG
Sbjct: 479 KSQNYYGGADFYNATNVVLPNGSLDPWHALGTYGTIKSQSLLPYLINGTAHCG 481

BLAST of Cp4.1LG01g06690 vs. NCBI nr
Match: XP_023550535.1 (probable serine protease EDA2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 484/529 (91.49%), Postives = 484/529 (91.49%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEGF
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGF 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. NCBI nr
Match: XP_022942800.1 (probable serine protease EDA2 [Cucurbita moschata])

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 482/529 (91.12%), Postives = 483/529 (91.30%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYL+TCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLLTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. NCBI nr
Match: KAG6600572.1 (putative serine protease EDA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 480/529 (90.74%), Postives = 483/529 (91.30%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFG+SYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGISYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLA+FNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAIFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYL+TCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLLTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. NCBI nr
Match: XP_022980600.1 (probable serine protease EDA2 [Cucurbita maxima])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 480/529 (90.74%), Postives = 480/529 (90.74%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYN H
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNHH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQE NQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQEINQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGD HSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDDHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. NCBI nr
Match: KAG7031212.1 (putative serine protease EDA2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 471/529 (89.04%), Postives = 474/529 (89.60%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFG+SYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGISYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLA+FNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAIFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQV         APANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQV---------APANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYL+TCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLLTCRNCGHGTDLR 475

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 475

BLAST of Cp4.1LG01g06690 vs. ExPASy TrEMBL
Match: A0A6J1FWY6 (probable serine protease EDA2 OS=Cucurbita moschata OX=3662 GN=LOC111447726 PE=3 SV=1)

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 482/529 (91.12%), Postives = 483/529 (91.30%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYL+TCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLLTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. ExPASy TrEMBL
Match: A0A6J1IWW4 (probable serine protease EDA2 OS=Cucurbita maxima OX=3661 GN=LOC111479920 PE=3 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 480/529 (90.74%), Postives = 480/529 (90.74%), Query Frame = 0

Query: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRH 60
           MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYN H
Sbjct: 1   MSKVVMVARLWLAVAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNHH 60

Query: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120
           KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS
Sbjct: 61  KFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKS 120

Query: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180
           SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW
Sbjct: 121 SPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGALSAW 180

Query: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFF 240
           FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ                             
Sbjct: 181 FQLKFPHLTCGSLASSAVVLAVFNFTDFDQQ----------------------------- 240

Query: 241 FFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300
                IGESAGPACKAALQE NQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA
Sbjct: 241 -----IGESAGPACKAALQEINQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAA 300

Query: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360
           MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE
Sbjct: 301 MAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGE 360

Query: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGF 420
           DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI           RYHLDLCKNVFGEG 
Sbjct: 361 DSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDI-----------RYHLDLCKNVFGEGL 420

Query: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480
           YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR
Sbjct: 421 YPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLR 480

Query: 481 GCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GCPQSPPNIEGD HSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV
Sbjct: 481 GCPQSPPNIEGDDHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 484

BLAST of Cp4.1LG01g06690 vs. ExPASy TrEMBL
Match: A0A6J1ENW5 (probable serine protease EDA2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436042 PE=3 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 1.50e-290
Identity = 399/534 (74.72%), Postives = 441/534 (82.58%), Query Frame = 0

Query: 1   MSKVVMVARLWL-AVAMAVFSSAFVNGHVT----LGWLSTRSEFLTQEEFWFDQTLDHFS 60
           M K++ + RLWL A A+A+  SAF  GHVT    L  LS+ S FLT+ E WF+QTLDHFS
Sbjct: 12  MGKILTIVRLWLVATAVALSLSAFTTGHVTPRTVLNRLSSSSSFLTRTEHWFNQTLDHFS 71

Query: 61  HYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHR 120
            YN  KFQQRY+EF DYFRIPDGPIF +ICGEGPCNGI+NDYL +LAKKFGAAIVSLEHR
Sbjct: 72  PYNHDKFQQRYYEFHDYFRIPDGPIFLKICGEGPCNGISNDYLGVLAKKFGAAIVSLEHR 131

Query: 121 YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSG 180
           YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKL++K ENPWF FGVSY G
Sbjct: 132 YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLNKKVENPWFFFGVSYPG 191

Query: 181 ALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYA 240
           ALSAWF+LKFPHLTCGSLASSAVVLAV+NFT+FDQQ                        
Sbjct: 192 ALSAWFRLKFPHLTCGSLASSAVVLAVYNFTEFDQQ------------------------ 251

Query: 241 RITFFFFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFL 300
                     IGESAGP CKAALQETN+LVEQRFA NK+EV+ALFGA E+E+DGDFFYFL
Sbjct: 252 ----------IGESAGPECKAALQETNRLVEQRFATNKKEVKALFGAGELEIDGDFFYFL 311

Query: 301 ADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKN 360
           ADAA +AFQYGNPD +C PLV+AKNAG DLVDAYAKYVK+Y++GSFG+NV+TY QKHLKN
Sbjct: 312 ADAAVIAFQYGNPDTVCSPLVQAKNAGNDLVDAYAKYVKDYYIGSFGTNVQTYNQKHLKN 371

Query: 361 TTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNV 420
           TT GEDSADRLWWFQVC+EVAYFQVAPANDS+RSSKVD            +YHLDLCKNV
Sbjct: 372 TTPGEDSADRLWWFQVCSEVAYFQVAPANDSMRSSKVDT-----------KYHLDLCKNV 431

Query: 421 FGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGH 480
           FGEG YPDVD+TNIYYGGTKIAGSKIVFTNGSQDPWRHASKQI SPDMPS+L+TC NCGH
Sbjct: 432 FGEGVYPDVDSTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQISSPDMPSFLITCHNCGH 491

Query: 481 GTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQASINRKYV 529
           GTDLRGCPQS  NIEGDAH+C+SP  V+KVRQQLV+KMDLWLSECQA+  R Y+
Sbjct: 492 GTDLRGCPQSHLNIEGDAHNCSSPDAVHKVRQQLVEKMDLWLSECQATTGRNYI 500

BLAST of Cp4.1LG01g06690 vs. ExPASy TrEMBL
Match: A0A6J1C577 (probable serine protease EDA2 OS=Momordica charantia OX=3673 GN=LOC111008474 PE=3 SV=1)

HSP 1 Score: 801 bits (2068), Expect = 5.86e-289
Identity = 404/533 (75.80%), Postives = 437/533 (81.99%), Query Frame = 0

Query: 1   MSKVVMVARLWLAV-AMAVFSSAFVNGHVTLGWLSTR--SEFLTQEEFWFDQTLDHFSHY 60
           M K V VARLWL V A+AV SS FVN H+ L  LST   S+FLTQ+EFWF QTLDHFS Y
Sbjct: 8   MGKAVAVARLWLLVTAVAVSSSPFVNAHLMLQRLSTTTSSKFLTQKEFWFPQTLDHFSPY 67

Query: 61  NRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYY 120
           NR+KFQQRYFEFLD+FR+PDGPIF +ICGEG CNGIANDYL++LAKKFGAAIVSLEHRYY
Sbjct: 68  NRNKFQQRYFEFLDHFRLPDGPIFLKICGEGTCNGIANDYLTVLAKKFGAAIVSLEHRYY 127

Query: 121 GKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSGAL 180
           GKSSPFKSLTTNNLRYLSSKQALFDLA FRQYYQDSLNLKL++K EN WF FGVSY GAL
Sbjct: 128 GKSSPFKSLTTNNLRYLSSKQALFDLAAFRQYYQDSLNLKLNKKGENMWFFFGVSYPGAL 187

Query: 181 SAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARI 240
           SAWF+LKFPHLTCGSLASSAVVLAV+NFT+FDQQV                         
Sbjct: 188 SAWFRLKFPHLTCGSLASSAVVLAVYNFTEFDQQV------------------------- 247

Query: 241 TFFFFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLAD 300
                    GESAGP CKAALQETN+LVEQRF  NK+EV+ALFGA EME+DGDFFYFLAD
Sbjct: 248 ---------GESAGPKCKAALQETNRLVEQRFVTNKKEVKALFGAAEMEIDGDFFYFLAD 307

Query: 301 AAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTT 360
           AA MAFQYGNPD+LC PLVEAKNAG DLVDAYAKYVK+YF+GSFG+NV+TY QKHLKNTT
Sbjct: 308 AAVMAFQYGNPDRLCSPLVEAKNAGNDLVDAYAKYVKDYFIGSFGTNVKTYNQKHLKNTT 367

Query: 361 LGEDSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFG 420
            G DSADRLWWFQVCTEVAYFQV+PANDSIRSSKVD            +YHLDLCKNVFG
Sbjct: 368 PGGDSADRLWWFQVCTEVAYFQVSPANDSIRSSKVDT-----------KYHLDLCKNVFG 427

Query: 421 EGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGT 480
           EG YPDV  TNIYYGGT+IAGSKI FTNG QDPWRHASKQ  SPDMPS+L+TC NCGHGT
Sbjct: 428 EGIYPDVAATNIYYGGTRIAGSKIFFTNGLQDPWRHASKQESSPDMPSFLITCHNCGHGT 487

Query: 481 DLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSEC-QASINRKYV 529
           DLRGCPQSP NIEGDAH+CTSP  V KVRQQLV+K+DLWLSEC Q    R+YV
Sbjct: 488 DLRGCPQSPLNIEGDAHNCTSPDAVQKVRQQLVEKIDLWLSECSQPKTGRQYV 495

BLAST of Cp4.1LG01g06690 vs. ExPASy TrEMBL
Match: A0A6J1ENA5 (probable serine protease EDA2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436042 PE=3 SV=1)

HSP 1 Score: 800 bits (2065), Expect = 1.81e-288
Identity = 397/528 (75.19%), Postives = 438/528 (82.95%), Query Frame = 0

Query: 1   MSKVVMVARLWL-AVAMAVFSSAFVNGHVT----LGWLSTRSEFLTQEEFWFDQTLDHFS 60
           M K++ + RLWL A A+A+  SAF  GHVT    L  LS+ S FLT+ E WF+QTLDHFS
Sbjct: 12  MGKILTIVRLWLVATAVALSLSAFTTGHVTPRTVLNRLSSSSSFLTRTEHWFNQTLDHFS 71

Query: 61  HYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHR 120
            YN  KFQQRY+EF DYFRIPDGPIF +ICGEGPCNGI+NDYL +LAKKFGAAIVSLEHR
Sbjct: 72  PYNHDKFQQRYYEFHDYFRIPDGPIFLKICGEGPCNGISNDYLGVLAKKFGAAIVSLEHR 131

Query: 121 YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLDRKSENPWFVFGVSYSG 180
           YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKL++K ENPWF FGVSY G
Sbjct: 132 YYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQDSLNLKLNKKVENPWFFFGVSYPG 191

Query: 181 ALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYA 240
           ALSAWF+LKFPHLTCGSLASSAVVLAV+NFT+FDQQ                        
Sbjct: 192 ALSAWFRLKFPHLTCGSLASSAVVLAVYNFTEFDQQ------------------------ 251

Query: 241 RITFFFFFFXIGESAGPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFL 300
                     IGESAGP CKAALQETN+LVEQRFA NK+EV+ALFGA E+E+DGDFFYFL
Sbjct: 252 ----------IGESAGPECKAALQETNRLVEQRFATNKKEVKALFGAGELEIDGDFFYFL 311

Query: 301 ADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKN 360
           ADAA +AFQYGNPD +C PLV+AKNAG DLVDAYAKYVK+Y++GSFG+NV+TY QKHLKN
Sbjct: 312 ADAAVIAFQYGNPDTVCSPLVQAKNAGNDLVDAYAKYVKDYYIGSFGTNVQTYNQKHLKN 371

Query: 361 TTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNV 420
           TT GEDSADRLWWFQVC+EVAYFQVAPANDS+RSSKVD            +YHLDLCKNV
Sbjct: 372 TTPGEDSADRLWWFQVCSEVAYFQVAPANDSMRSSKVDT-----------KYHLDLCKNV 431

Query: 421 FGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGH 480
           FGEG YPDVD+TNIYYGGTKIAGSKIVFTNGSQDPWRHASKQI SPDMPS+L+TC NCGH
Sbjct: 432 FGEGVYPDVDSTNIYYGGTKIAGSKIVFTNGSQDPWRHASKQISSPDMPSFLITCHNCGH 491

Query: 481 GTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLVKKMDLWLSECQAS 523
           GTDLRGCPQS  NIEGDAH+C+SP  V+KVRQQLV+KMDLWLSECQA+
Sbjct: 492 GTDLRGCPQSHLNIEGDAHNCSSPDAVHKVRQQLVEKMDLWLSECQAT 494

BLAST of Cp4.1LG01g06690 vs. TAIR 10
Match: AT4G36190.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 644.4 bits (1661), Expect = 7.8e-185
Identity = 310/491 (63.14%), Postives = 368/491 (74.95%), Query Frame = 0

Query: 33  LSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNG 92
           LS  S++LT++E WF QTLDH+S  +  KF+QRY+E+LD+ R+PDGPIF  ICGEGPCNG
Sbjct: 36  LSKSSKYLTRDELWFTQTLDHYSPSDHRKFRQRYYEYLDHLRVPDGPIFLMICGEGPCNG 95

Query: 93  IANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQD 152
           I N+Y+S+LAKKF A IVSLEHRYYGKSSPFKSL T NL+YLSSKQAL DLA FRQYYQD
Sbjct: 96  ITNNYISVLAKKFDAGIVSLEHRYYGKSSPFKSLATKNLKYLSSKQALSDLATFRQYYQD 155

Query: 153 SLNLKLDRKS--ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQ 212
           SLN+K +R S  ENPWF FGVSYSGALSAWF+LKFPHLTCGSLASSAVV AV+ F +FDQ
Sbjct: 156 SLNVKFNRSSNVENPWFFFGVSYSGALSAWFRLKFPHLTCGSLASSAVVRAVYEFPEFDQ 215

Query: 213 QVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFA 272
           Q                                  I ESAGP C+ ALQETN+L+E    
Sbjct: 216 Q----------------------------------IAESAGPECETALQETNKLLELGLK 275

Query: 273 INKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYA 332
           +N   V+ALF A E++VD DF Y +ADA  MA QYGNPDKLC PLVEA+  G DLV+AYA
Sbjct: 276 VNNRAVKALFNATELDVDADFLYLIADAGVMAIQYGNPDKLCVPLVEAQKNGGDLVEAYA 335

Query: 333 KYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSS 392
           KYV+ + +G FG + +TY +KHL +T +  +SADRLWWFQVCTEVAYFQVAPANDSIRS 
Sbjct: 336 KYVREFCMGVFGQSSKTYSRKHLLDTAVTLESADRLWWFQVCTEVAYFQVAPANDSIRSH 395

Query: 393 KVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDP 452
           ++           N  YHLDLCK++FG+G YP+VD TN+YYG  KIA +KI+FTNGSQDP
Sbjct: 396 QI-----------NTEYHLDLCKSLFGKGVYPEVDATNLYYGSDKIAATKIIFTNGSQDP 455

Query: 453 WRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLV 512
           WRHASKQ  SPD+PSY++TC NCGHG+DLRGCPQS   IEGDA +C+SP  V KVRQ ++
Sbjct: 456 WRHASKQTSSPDLPSYIMTCHNCGHGSDLRGCPQSAMVIEGDAQNCSSPDAVNKVRQHMI 481

Query: 513 KKMDLWLSECQ 522
           K +DLWLSEC+
Sbjct: 516 KHIDLWLSECR 481

BLAST of Cp4.1LG01g06690 vs. TAIR 10
Match: AT4G36195.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-184
Identity = 309/494 (62.55%), Postives = 369/494 (74.70%), Query Frame = 0

Query: 33  LSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNG 92
           L+  S++LT++E WF+QTLDH+S  +  +F+QRY+E+LD+ R+PDGPIF  ICGEGPCNG
Sbjct: 36  LTESSKYLTRDELWFNQTLDHYSPSDHREFKQRYYEYLDHLRVPDGPIFMMICGEGPCNG 95

Query: 93  IANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQD 152
           I NDY+++LAKKF A IVSLEHRYYGKSSPFKSL T NL+YLSSKQALFDLA FRQYYQD
Sbjct: 96  IPNDYITVLAKKFDAGIVSLEHRYYGKSSPFKSLATENLKYLSSKQALFDLAAFRQYYQD 155

Query: 153 SLNLKLDRKS--ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQ 212
           SLN+K +R    ENPWF FG SYSGALSAWF+LKFPHLTCGSLASSAVV AV+ F +FDQ
Sbjct: 156 SLNVKFNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASSAVVRAVYEFPEFDQ 215

Query: 213 QVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFA 272
           Q                                  IGESAGP CKAALQETN+L+E    
Sbjct: 216 Q----------------------------------IGESAGPECKAALQETNKLLELGLK 275

Query: 273 INKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYA 332
           +N   V+ALF A E++VD DF Y +ADA  MA QYGNPDKLC PLVEA+    DLV+AYA
Sbjct: 276 VNNRAVKALFNATELDVDADFLYLIADAEVMAIQYGNPDKLCVPLVEAQKNRDDLVEAYA 335

Query: 333 KYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSS 392
           KYV+ + +G FG + +TY +KHL +T +  +SADRLWWFQVCTEVAYFQVAPANDSIRS 
Sbjct: 336 KYVREFCVGVFGLSSKTYSRKHLLDTAVTPESADRLWWFQVCTEVAYFQVAPANDSIRSH 395

Query: 393 KVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDP 452
           ++           N  YHLDLCK++FG+G YP+VD TN+YYG  +IA +KI+FTNGSQDP
Sbjct: 396 QI-----------NTEYHLDLCKSLFGKGVYPEVDATNLYYGSDRIAATKIIFTNGSQDP 455

Query: 453 WRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLV 512
           WRHASKQ  SP++PSY+VTC NCGHG+DLRGCPQSP  I GD+ +C+SP  V KVRQ +V
Sbjct: 456 WRHASKQTSSPELPSYIVTCHNCGHGSDLRGCPQSPMVIGGDSKNCSSPDAVNKVRQHIV 484

Query: 513 KKMDLWLSECQASI 525
             MDLWLSEC+  I
Sbjct: 516 DHMDLWLSECRGGI 484

BLAST of Cp4.1LG01g06690 vs. TAIR 10
Match: AT4G36195.3 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-184
Identity = 309/494 (62.55%), Postives = 369/494 (74.70%), Query Frame = 0

Query: 33  LSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNG 92
           L+  S++LT++E WF+QTLDH+S  +  +F+QRY+E+LD+ R+PDGPIF  ICGEGPCNG
Sbjct: 36  LTESSKYLTRDELWFNQTLDHYSPSDHREFKQRYYEYLDHLRVPDGPIFMMICGEGPCNG 95

Query: 93  IANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQD 152
           I NDY+++LAKKF A IVSLEHRYYGKSSPFKSL T NL+YLSSKQALFDLA FRQYYQD
Sbjct: 96  IPNDYITVLAKKFDAGIVSLEHRYYGKSSPFKSLATENLKYLSSKQALFDLAAFRQYYQD 155

Query: 153 SLNLKLDRKS--ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQ 212
           SLN+K +R    ENPWF FG SYSGALSAWF+LKFPHLTCGSLASSAVV AV+ F +FDQ
Sbjct: 156 SLNVKFNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASSAVVRAVYEFPEFDQ 215

Query: 213 QVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFA 272
           Q                                  IGESAGP CKAALQETN+L+E    
Sbjct: 216 Q----------------------------------IGESAGPECKAALQETNKLLELGLK 275

Query: 273 INKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYA 332
           +N   V+ALF A E++VD DF Y +ADA  MA QYGNPDKLC PLVEA+    DLV+AYA
Sbjct: 276 VNNRAVKALFNATELDVDADFLYLIADAEVMAIQYGNPDKLCVPLVEAQKNRDDLVEAYA 335

Query: 333 KYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSS 392
           KYV+ + +G FG + +TY +KHL +T +  +SADRLWWFQVCTEVAYFQVAPANDSIRS 
Sbjct: 336 KYVREFCVGVFGLSSKTYSRKHLLDTAVTPESADRLWWFQVCTEVAYFQVAPANDSIRSH 395

Query: 393 KVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDP 452
           ++           N  YHLDLCK++FG+G YP+VD TN+YYG  +IA +KI+FTNGSQDP
Sbjct: 396 QI-----------NTEYHLDLCKSLFGKGVYPEVDATNLYYGSDRIAATKIIFTNGSQDP 455

Query: 453 WRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLV 512
           WRHASKQ  SP++PSY+VTC NCGHG+DLRGCPQSP  I GD+ +C+SP  V KVRQ +V
Sbjct: 456 WRHASKQTSSPELPSYIVTCHNCGHGSDLRGCPQSPMVIGGDSKNCSSPDAVNKVRQHIV 484

Query: 513 KKMDLWLSECQASI 525
             MDLWLSEC+  I
Sbjct: 516 DHMDLWLSECRGGI 484

BLAST of Cp4.1LG01g06690 vs. TAIR 10
Match: AT4G36195.2 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 622.5 bits (1604), Expect = 3.2e-178
Identity = 304/494 (61.54%), Postives = 361/494 (73.08%), Query Frame = 0

Query: 33  LSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYFRIPDGPIFFRICGEGPCNG 92
           L+  S++LT++E WF+QTLDH+S  +  +F+QRY+E+LD+ R+PDGPIF  ICGEGPCNG
Sbjct: 36  LTESSKYLTRDELWFNQTLDHYSPSDHREFKQRYYEYLDHLRVPDGPIFMMICGEGPCNG 95

Query: 93  IANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRYLSSKQALFDLAVFRQYYQD 152
           I NDY+++LAKKF A IVSLEHRYYGKSSPFKSL T NL+YLSSKQALFDLA FRQYYQD
Sbjct: 96  IPNDYITVLAKKFDAGIVSLEHRYYGKSSPFKSLATENLKYLSSKQALFDLAAFRQYYQD 155

Query: 153 SLNLKLDRKS--ENPWFVFGVSYSGALSAWFQLKFPHLTCGSLASSAVVLAVFNFTDFDQ 212
           SLN+K +R    ENPWF FG SYSGALSAWF+LKFPHLTCGSLASSAVV AV+ F +FDQ
Sbjct: 156 SLNVKFNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASSAVVRAVYEFPEFDQ 215

Query: 213 QVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESAGPACKAALQETNQLVEQRFA 272
           Q                                  IGESAGP CKAALQETN+L+E    
Sbjct: 216 Q----------------------------------IGESAGPECKAALQETNKLLELGLK 275

Query: 273 INKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDKLCCPLVEAKNAGKDLVDAYA 332
           +N   V+ALF A E++VD DF Y +ADA  MA QYGNPDKLC PLVEA+    DLV+AYA
Sbjct: 276 VNNRAVKALFNATELDVDADFLYLIADAEVMAIQYGNPDKLCVPLVEAQKNRDDLVEAYA 335

Query: 333 KYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQVCTEVAYFQVAPANDSIRSS 392
           KYV+ + +G FG + +TY +KHL +T +  +SADRLWWFQVCTEVAYFQVAPANDSIRS 
Sbjct: 336 KYVREFCVGVFGLSSKTYSRKHLLDTAVTPESADRLWWFQVCTEVAYFQVAPANDSIRSH 395

Query: 393 KVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIYYGGTKIAGSKIVFTNGSQDP 452
           ++           N  YHLDLCK++FG+G YP+VD TN+YYG  +IA +KI+FTNGSQDP
Sbjct: 396 QI-----------NTEYHLDLCKSLFGKGVYPEVDATNLYYGSDRIAATKIIFTNGSQDP 455

Query: 453 WRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIEGDAHSCTSPATVYKVRQQLV 512
           WRHASKQ  SP++PSY+VTC NCGHG+DLRGCPQSP  I            V KVRQ +V
Sbjct: 456 WRHASKQTSSPELPSYIVTCHNCGHGSDLRGCPQSPMVI-----------GVNKVRQHIV 473

Query: 513 KKMDLWLSECQASI 525
             MDLWLSEC+  I
Sbjct: 516 DHMDLWLSECRGGI 473

BLAST of Cp4.1LG01g06690 vs. TAIR 10
Match: AT2G18080.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.5e-172
Identity = 294/514 (57.20%), Postives = 366/514 (71.21%), Query Frame = 0

Query: 14  VAMAVFSSAFVNGHVTLGWLSTRSEFLTQEEFWFDQTLDHFSHYNRHKFQQRYFEFLDYF 73
           V+ +  S A ++       +S    ++T  E WF+QTLDH S  +  KF+QRY+EF+DYF
Sbjct: 17  VSFSTLSHALLHPSSVSHNVSRSRYYMTTNELWFNQTLDHESPNDHRKFRQRYYEFMDYF 76

Query: 74  RIPDGPIFFRICGEGPCNGIANDYLSILAKKFGAAIVSLEHRYYGKSSPFKSLTTNNLRY 133
           R PDGP+F  ICGEGPC+GIANDY+++LAKKF A +VSLEHRYYGKSSPF SL T NL+Y
Sbjct: 77  RSPDGPMFMIICGEGPCSGIANDYINVLAKKFQAGVVSLEHRYYGKSSPFNSLATENLKY 136

Query: 134 LSSKQALFDLAVFRQYYQDSLNLKLDRK---SENPWFVFGVSYSGALSAWFQLKFPHLTC 193
           LSSKQAL+DLA FRQYYQ+SLN KL+     S+NPWF FG+SYSGALSAWF+LKFPHLTC
Sbjct: 137 LSSKQALYDLASFRQYYQESLNKKLNISSGGSDNPWFFFGISYSGALSAWFRLKFPHLTC 196

Query: 194 GSLASSAVVLAVFNFTDFDQQVIQISCYWLYAFLLSDSINFRFYARITFFFFFFXIGESA 253
           GSLASSAVV A++ F++FDQQ                                  IGESA
Sbjct: 197 GSLASSAVVRAIYEFSEFDQQ----------------------------------IGESA 256

Query: 254 GPACKAALQETNQLVEQRFAINKEEVQALFGAEEMEVDGDFFYFLADAAAMAFQYGNPDK 313
           G  CK ALQETN+L+E    +  + V++LF A E++VD DF Y  ADAA MAFQYGNPDK
Sbjct: 257 GQECKLALQETNKLLELGLKVKNKAVKSLFNATELDVDADFLYLTADAAVMAFQYGNPDK 316

Query: 314 LCCPLVEAKNAGKDLVDAYAKYVKNYFLGSFGSNVRTYIQKHLKNTTLGEDSADRLWWFQ 373
           LC PLVEAK  G DLV  Y+ YV+ Y +  +G  VRTY +KHL+NT +  DSA RLWWFQ
Sbjct: 317 LCVPLVEAKKNGSDLVVTYSTYVREYCMRIWGLRVRTYNRKHLRNTVVTADSAYRLWWFQ 376

Query: 374 VCTEVAYFQVAPANDSIRSSKVDISYLFTNNSSNHRYHLDLCKNVFGEGFYPDVDTTNIY 433
            CTE+ YFQVAP  DS+RS ++           N  +HLDLCK++FG+  YP VD TN+Y
Sbjct: 377 ACTELGYFQVAPKYDSVRSHQI-----------NTTFHLDLCKSLFGKDVYPKVDATNLY 436

Query: 434 YGGTKIAGSKIVFTNGSQDPWRHASKQIPSPDMPSYLVTCRNCGHGTDLRGCPQSPPNIE 493
           YGG ++A +KI+FTNGS+DPWRHASKQ  + +MPSY++ CRNCGHG+D+RGCPQSP  IE
Sbjct: 437 YGGDRLAATKIIFTNGSEDPWRHASKQNSTHEMPSYIIKCRNCGHGSDIRGCPQSPMVIE 485

Query: 494 GDAHSCTSPATVYKVRQQLVKKMDLWLSECQASI 525
           G +++C+ P  V KVRQQ+V+ +DLWLSEC+ SI
Sbjct: 497 GKSNNCSLPDYVNKVRQQMVEHIDLWLSECRQSI 485