Cp4.1LG01g06560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g06560
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
LocationCp4.1LG01: 130513 .. 133535 (+)
RNA-Seq ExpressionCp4.1LG01g06560
SyntenyCp4.1LG01g06560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAGGGCATAAAAGACATTCCCCCGTCCTCTTCCGATGCTGCCCTAATTCCTGGTGTATAAACTTGTTGGAACTGGTACACCGTAATTTTTGCTTCATTTTCTGGGCTTCTCGCAAATGAACATTTTGAAGCATCCAATTATCAGCAGGCGAGGATTCAGATATGGTGGAGGTACTGTATGTTCATCTCTAATTTTGAGACTCTGGTTTTTGGATTCTCGTTCAAATGTTAGACGCACATTCGTTTCTGTTCCCCCTTTTTCTTTTCTGCTTACCTCATGTATTTGCTTGATCGACTTTTTTGGATTACAATGGTTTCAAAATAGAGCGAGGGGTTGGAGTAAGTAAATATTAGTTTTTGATTCCTCTGTCCAGTCATTATGATGATTCAGTAGTCAAAATTGTTCAAATCCAGCAAATGGATAGTTAATGTGCTCCAAATGACTATGGAGAAAGAATAATTTTTTCTCTGGCCTCTTGATAAGTTCTACATTCTGCTGAGCCTTTTTTTTTTAGACTAAGAATTTAAGTAGTGAAAGAAAGCTCCTCCCGCTTGAATTAGCATCTCAGCTTGTTGCTGATGAACCATTTGTACATTGTAGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTAGAGAGCAAGAAGTAGTGATTGCTTTAGGGAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCAGTTGATGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTAGATATCTTGTTGTATGGAAGATATAAAATACACTCAGATACTCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCGTTTGTGCTGGCTCCTTTGATTGATTTGCTGGGTTCAGATGTTGATACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGGCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGGGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCACAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGAAATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGCTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGCGAAGTATGTTCGCAACCGATTGCAACGAAGAGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGAAAACAAGCTGAAGCATGATTGTTTCTCGTTACACATCCTTTTCATTCTCATTTTTTTAATCAAATTATTATTTTTGGATAATTTGGTACACCGCTCATGCTTTTACTGCTAGCGTCACAAGGAATAAAATGAGTTACGCGATGCCCCCTGGAATAGTATTATCTCAAGTTATTGGTTGTCATTGATCCATCGGAAGATTGTCTGTCTCTCACTAGTGCCCCTAGGAGTATTTGTTTTCAAGGTTGCCTTTAAATATCATTTGAGTAAAGGTATTGGCACTCTAAATGTCTGTTGGAATATTATTATCTGAAAACTTAACCAACAAATACAATGACATCCATTCAAAGTAATGGAAGAACTAATATAAAGAGATTAGAGAAAGAGAGAGTATACACAACTTAGTTCCTCCTTTCTTTGGTAAAAGGGAATTATCTGGTAACTGACGAAGCATGTAAATATCTGATAATTTCAAGAGGCCTAGAAAGTGATCAGGTCAGGTAAGTCTTGTTTATGTACTGGTTTAGAAACAAGGCTCAAATGGGAAGAAGAACAGCTTGCAAAAGGGGCTTGTAAAGTGGTTGATGAACAACAATCTGCATCAAATCTCTCCCATTTGTCTGTAATCACTGTCTTCAACCCCCTCTTTGATCCATTTCCAAGCTCGCTCCTCATCTCTCCTCCATTATCTTTCCCTTTTAAATTAAGGCTTCCATTGATAATTTTCTCAAGATCTTTGATATCTTCTTCTGGGATCTTCTCCAATTTTAGGCTCTGAGATGCATTCAAAACACCCATTTCTCTACATATCTCAAAGTATTGAGTCAAATCTTCTACGTGAGTTGCTGCTTTTTTGACAACTTGAAGTGCCAATGATGCTTCGGGTTTCGCTGGTGTCTCGTAAATGTTCAACAGAACTTGAGCAATTCCGTTGCAGATTTGGCTGTAAACGTCAAAAATCTCAAAGATGA

mRNA sequence

CTCAAGGGCATAAAAGACATTCCCCCGTCCTCTTCCGATGCTGCCCTAATTCCTGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTAGAGAGCAAGAAGTAGTGATTGCTTTAGGGAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCAGTTGATGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTAGATATCTTGTTGTATGGAAGATATAAAATACACTCAGATACTCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCGTTTGTGCTGGCTCCTTTGATTGATTTGCTGGGTTCAGATGTTGATACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGGCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGGGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCACAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGAAATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGCTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGCGAAGTATGTTCGCAACCGATTGCAACGAAGAGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGAAAACAAGCTGAAGCATGATTGTTTCTCGTTACACATCCTTTTCATTCTCATTTTTTTAATCAAATTATTATTTTTGGATAATTTGGTACACCGCTCATGCTTTTACTGCTAGCGTCACAAGGAATAAAATGAGTTACGCGATGCCCCCTGGAATAGTATTATCTCAAGTTATTGGTTGTCATTGATCCATCGGAAGATTGTCTGTCTCTCACTAGTGCCCCTAGGAGTATTTGTTTTCAAGGTTGCCTTTAAATATCATTTGAGTAAAGGTATTGGCACTCTAAATGTCTGTTGGAATATTATTATCTGAAAACTTAACCAACAAATACAATGACATCCATTCAAAGTAATGGAAGAACTAATATAAAGAGATTAGAGAAAGAGAGAGTATACACAACTTAGTTCCTCCTTTCTTTGGTAAAAGGGAATTATCTGGTAACTGACGAAGCATGTAAATATCTGATAATTTCAAGAGGCCTAGAAAGTGATCAGGTCAGGTAAGTCTTGTTTATGTACTGGTTTAGAAACAAGGCTCAAATGGGAAGAAGAACAGCTTGCAAAAGGGGCTTGTAAAGTGGTTGATGAACAACAATCTGCATCAAATCTCTCCCATTTGTCTGTAATCACTGTCTTCAACCCCCTCTTTGATCCATTTCCAAGCTCGCTCCTCATCTCTCCTCCATTATCTTTCCCTTTTAAATTAAGGCTTCCATTGATAATTTTCTCAAGATCTTTGATATCTTCTTCTGGGATCTTCTCCAATTTTAGGCTCTGAGATGCATTCAAAACACCCATTTCTCTACATATCTCAAAGTATTGAGTCAAATCTTCTACGTGAGTTGCTGCTTTTTTGACAACTTGAAGTGCCAATGATGCTTCGGGTTTCGCTGGTGTCTCGTAAATGTTCAACAGAACTTGAGCAATTCCGTTGCAGATTTGGCTGTAAACGTCAAAAATCTCAAAGATGA

Coding sequence (CDS)

CTCAAGGGCATAAAAGACATTCCCCCGTCCTCTTCCGATGCTGCCCTAATTCCTGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTAGAGAGCAAGAAGTAGTGATTGCTTTAGGGAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCAGTTGATGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTAGATATCTTGTTGTATGGAAGATATAAAATACACTCAGATACTCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCGTTTGTGCTGGCTCCTTTGATTGATTTGCTGGGTTCAGATGTTGATACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGGCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGGGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCACAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGAAATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGCTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGCGAAGTATGTTCGCAACCGATTGCAACGAAGAGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGAAAACAAGCTGA

Protein sequence

LKGIKDIPPSSSDAALIPALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLKEKTS
Homology
BLAST of Cp4.1LG01g06560 vs. ExPASy Swiss-Prot
Match: O04862 (Folate synthesis bifunctional protein, mitochondrial OS=Pisum sativum OX=3888 GN=MitHPPK/DHPS PE=1 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.6e-208
Identity = 344/489 (70.35%), Postives = 424/489 (86.71%), Query Frame = 0

Query: 17  IPALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHAC 76
           +  L  S  H++ ++ +E+ ++++EVVIALGSNVGDRL NF  AL+LM+K+GIHITRHA 
Sbjct: 21  LKVLGFSSFHTAPNSSIEIQTQDEEVVIALGSNVGDRLHNFKEALKLMRKSGIHITRHAS 80

Query: 77  LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDIL 136
           LYETAPAYVT+QP+FLNSAVRA TKLGPHELL+A+K IEK +GRT GIRYGPRPIDLDIL
Sbjct: 81  LYETAPAYVTDQPRFLNSAVRADTKLGPHELLAALKRIEKDMGRTDGIRYGPRPIDLDIL 140

Query: 137 LYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWE 196
            YG++K+ SD LTVPHERIWERPFV+APL+DLLG+ +D+D VA WHS +   GGL  LWE
Sbjct: 141 FYGKFKVRSDILTVPHERIWERPFVMAPLMDLLGTAIDSDTVASWHSFSGHSGGLNALWE 200

Query: 197 KMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVR 256
           K+GGESLIG+EGM RV+P+ N L DWS +T +MG+LNLTPDSFSDGG FQ +++AVSQ R
Sbjct: 201 KLGGESLIGEEGMYRVMPVANGLLDWSRRTLVMGILNLTPDSFSDGGNFQSVKSAVSQAR 260

Query: 257 SMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 316
            M+S+GAD+IDIGAQSTRPMA  IS EEEL RL+PVLEAV  +PE+ GKLISVDTFYSEV
Sbjct: 261 LMISEGADIIDIGAQSTRPMASRISAEEELGRLIPVLEAVMSIPEVEGKLISVDTFYSEV 320

Query: 317 ALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN 376
           ALEAV++GAHI+NDVSAG+LD  M +V+A+L VPY+AMHMRGDPSTMQ++ENL+YD+VC 
Sbjct: 321 ALEAVRKGAHIINDVSAGKLDASMFKVMAELDVPYVAMHMRGDPSTMQDSENLKYDNVCK 380

Query: 377 EIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLS 436
           +I+SEL+SR+R+AE+SGIPAWRII+DPGIGFSK T+ NL  L G+P IR EI++RSL +S
Sbjct: 381 DISSELYSRVREAEISGIPAWRIIMDPGIGFSKKTEDNLAALTGIPDIREEISKRSLAIS 440

Query: 437 HAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLC 496
           HAP+LIGPSRK+FLGE+CS+P A  RDPAT+A+VT GVL GANI RVHNV+DN+DAV+LC
Sbjct: 441 HAPILIGPSRKRFLGEICSRPSAVDRDPATIASVTAGVLCGANIVRVHNVKDNLDAVKLC 500

Query: 497 DAMLKEKTS 506
           DA+LK+K+S
Sbjct: 501 DAILKQKSS 509

BLAST of Cp4.1LG01g06560 vs. ExPASy Swiss-Prot
Match: Q9SZV3 (Folate synthesis bifunctional protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MitHPPK/DHPS PE=2 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 1.9e-201
Identity = 336/489 (68.71%), Postives = 411/489 (84.05%), Query Frame = 0

Query: 18  PAL-QSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHAC 77
           PAL  S+F  S+    +EV S E EVVIALGSN+G+R+ NF  AL+LMK+ GI +TRH+C
Sbjct: 64  PALCNSAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSC 123

Query: 78  LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDIL 137
           LYETAP +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL
Sbjct: 124 LYETAPVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDIL 183

Query: 138 LYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWE 197
            YG+ +I SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE
Sbjct: 184 FYGKMRISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWE 243

Query: 198 KMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVR 257
           ++GGESLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VR
Sbjct: 244 RLGGESLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVR 303

Query: 258 SMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 317
           SM+S+GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEV
Sbjct: 304 SMISEGADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEV 363

Query: 318 ALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN 377
           A EA+  GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC 
Sbjct: 364 ASEAISNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCK 423

Query: 378 EIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLS 437
           ++ASEL+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +S
Sbjct: 424 DVASELYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVS 483

Query: 438 HAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLC 497
           HAP+L+GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++C
Sbjct: 484 HAPILVGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKVC 543

Query: 498 DAMLKEKTS 506
           DAML+ + S
Sbjct: 544 DAMLRRRRS 552

BLAST of Cp4.1LG01g06560 vs. ExPASy Swiss-Prot
Match: Q1ENB6 (Folate synthesis bifunctional protein OS=Arabidopsis thaliana OX=3702 GN=CytHPPK/DHPS PE=1 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.4e-188
Identity = 313/468 (66.88%), Postives = 396/468 (84.62%), Query Frame = 0

Query: 40  QEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAV 99
           +EVVIALGSNVG+R+ NF  AL+LMK  GI +TRH+CLYET P +VT+QP+FLN+A+R V
Sbjct: 12  EEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGV 71

Query: 100 TKLGPHELLSAVKNIEKQLGRTA-GIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWER 159
           TKL PHELL+ +K IEK++GR   G+RYGPRP+DLDIL YG++KI SD L +PHERIWER
Sbjct: 72  TKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWER 131

Query: 160 PFVLAPLIDLLGS-DVDTDD-VACWHSLAADRGGLFELWEKMGGESLIGKEG-MRRVLPI 219
           PFVLAPL+DLLG+ D+D D  VA WHSL+   GG+F+ WE++GGESL+GK+G ++RV+PI
Sbjct: 132 PFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPI 191

Query: 220 GNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRP 279
           G++LWD+S KT +MG+LNLTPDSFSDGGKFQ ++ AVS+VRSM+S+G D+IDIGAQSTRP
Sbjct: 192 GDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRP 251

Query: 280 MAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQ 339
           MA  IS +EE+DRL+PVL+ V GM EM GKLISVDTF SEVALEA++ GA I+NDVS G 
Sbjct: 252 MASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGS 311

Query: 340 LDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIP 399
           LD  MH+VVA   VPY+ MHMRGDP TMQN ENL+Y+++C ++A+EL+ R+R+AELSGIP
Sbjct: 312 LDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIP 371

Query: 400 AWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCS 459
           AWRI++DPGIGFSK    NL+I+  +PKIR E+A++S+GLSHAP+LIGPSRK+FLG++C 
Sbjct: 372 AWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICG 431

Query: 460 QPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLKEK 504
           +P A++RD ATVA VT G+L GANI RVHNVRDNVDA RLCDAM+ ++
Sbjct: 432 RPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMMTKR 479

BLAST of Cp4.1LG01g06560 vs. ExPASy Swiss-Prot
Match: P29251 (Folic acid synthesis protein fol1 OS=Pneumocystis carinii OX=4754 GN=fol1 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.1e-83
Identity = 194/500 (38.80%), Postives = 286/500 (57.20%), Query Frame = 0

Query: 12  SDAALIPALQSSFIHSSQDAVLEVCSREQEVV-IALGSNVGDRLQNFNGALQLMKKAGIH 71
           +++A +  ++S    SS + +    S + E V I+LGSN+G+R++    A++ M   GI 
Sbjct: 265 AESAGVEIVRSRSCFSSNNYIKSENSIDNEAVYISLGSNLGNRIKFILDAIEKMSIKGIK 324

Query: 72  ITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRP 131
           + + + LYE+ P Y  +QP F N+  +  T L P +LL  ++ IEK+LGR   I  GPR 
Sbjct: 325 VLKTSMLYESKPMYFKDQPAFYNAVCKVQTSLHPEQLLFELQLIEKELGRVKVIDKGPRC 384

Query: 132 IDLDILLYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGG 191
           IDLDI+ YGR  I+S++L +PH R+ ER FVL PL+D+ G  V        H +      
Sbjct: 385 IDLDIVFYGRKIINSESLIIPHPRVLERSFVLKPLLDISGDLV--------HPVTG--LS 444

Query: 192 LFELWEKMGGESLIGKEGMRRVLP-------IGNNLWDWSCKTSIMGVLNLTPDSFSDGG 251
           +   +EK      I    ++ VLP       I  +   +   T IM +LNLTPDSF DGG
Sbjct: 445 IASYFEK------IVDHDIKPVLPFLYKNKSIDFSFRSYKAPTYIMAILNLTPDSFFDGG 504

Query: 252 KFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAV-TGMPEM 311
                ++ +  V   ++ GA +IDIG QSTRP + +I +EEE+ R++P ++ +    P++
Sbjct: 505 -IHSYDSVLIDVEKFINAGATIIDIGGQSTRPGSYIIPLEEEIFRVIPAIKYLQKTYPDI 564

Query: 312 SGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPST 371
              LIS+DTF SEVA +AVK GA +VND+S G+ DP+M   VA+LKVP   MHMRG+   
Sbjct: 565 ---LISIDTFRSEVAEQAVKAGASLVNDISGGRYDPKMFNTVARLKVPICIMHMRGNFLN 624

Query: 372 MQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVP 431
           M N  +    D+  +I  EL   +  AE SGIP W II+DPG+GFSK   QN+E+L    
Sbjct: 625 MDNLTDYG-TDIIEQITIELEKLLNSAEKSGIPRWNIILDPGLGFSKTLHQNIELLRRFN 684

Query: 432 KIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIAR 491
           +++++     L     P L+GPSRK+F G +    +   R   TVAAV   + GG +I R
Sbjct: 685 ELKSKNCFNGL-----PWLLGPSRKRFTGFITGDNMPKDRIWGTVAAVVASISGGCDIIR 738

Query: 492 VHNVRDNVDAVRLCDAMLKE 503
           VH+V +     ++ DA+ KE
Sbjct: 745 VHDVYEMYKISKMSDAIWKE 738

BLAST of Cp4.1LG01g06560 vs. ExPASy Swiss-Prot
Match: Q4LB35 (Folic acid synthesis protein fol1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fol1 PE=1 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 174/459 (37.91%), Postives = 255/459 (55.56%), Query Frame = 0

Query: 44  IALGSNVGDRLQNFNGALQLMKK-AGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKL 103
           ++ GSN+GD+ +    AL ++ K  GI +   + LYET P Y  +QP FLN   +  T++
Sbjct: 300 LSFGSNIGDKFEQIQTALSMLHKIEGIRVLDVSPLYETEPMYYKDQPSFLNGVCKIETRM 359

Query: 104 GPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWERPFVL 163
            P  LL A ++IE+++GR   I  GPR IDLDI+LY      S+ LT+PH  + ER FVL
Sbjct: 360 SPINLLRACQSIEQEMGRIKTILKGPRCIDLDIVLYEDCVYESEVLTIPHLGLQEREFVL 419

Query: 164 APLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDW 223
            PL+ L      + D+   ++       L E  +K      +  +G+R      N     
Sbjct: 420 RPLLAL------SPDLVHPYT----HQPLQEALDK------LPSQGIRLYSSFDNKKIIN 479

Query: 224 SCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISV 283
              T  MG+LN+TPDSFSDGGK       + + +SMV DGA ++DIG QST+P A  +SV
Sbjct: 480 GALT--MGILNVTPDSFSDGGKVSQ-NNILEKAKSMVGDGASILDIGGQSTKPGADPVSV 539

Query: 284 EEELDRLVPVLEAVTGMPEMSGKL--ISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQM 343
           EEEL R++P++  +      SG    IS+DT+YS+VA  A++ GA+I+NDV+ G  D +M
Sbjct: 540 EEELRRVIPMISLL----RSSGITVPISIDTYYSKVAKLAIEAGANIINDVTGGMGDEKM 599

Query: 344 HRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRII 403
             + A L+VP   MHMRG P TM+   ++   D+  E+A EL SR+  A  SG+  + II
Sbjct: 600 LPLAASLQVPICIMHMRGTPETMK-ALSIYEKDIVEEVAVELSSRVEAAVQSGVHRYNII 659

Query: 404 VDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIAT 463
           +DPG GF+K  KQ+  +LG + ++  +   + +       L GPSRK F G         
Sbjct: 660 LDPGFGFAKTPKQSAGLLGRLHELMKKPQFKDM-----HWLSGPSRKGFTGYFTGDASPK 719

Query: 464 KRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAM 500
            R   T A VT  VL G +I RVH+ ++    V + +A+
Sbjct: 720 DRIWGTSACVTASVLQGVSIVRVHDTKEMSKVVGMANAI 729

BLAST of Cp4.1LG01g06560 vs. NCBI nr
Match: XP_023536333.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78
           ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY
Sbjct: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78

Query: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138
           ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY
Sbjct: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138

Query: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198
           GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM
Sbjct: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198

Query: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258
           GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM
Sbjct: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258

Query: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318
           VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL
Sbjct: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318

Query: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378
           EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI
Sbjct: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378

Query: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438
           ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA
Sbjct: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438

Query: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498
           PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA
Sbjct: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498

Query: 499 MLKEKTS 505
           MLKEKTS
Sbjct: 499 MLKEKTS 505

BLAST of Cp4.1LG01g06560 vs. NCBI nr
Match: XP_022990585.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 966 bits (2497), Expect = 0.0
Identity = 482/487 (98.97%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78
           ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGAL+L+KKAGIHITRHACLY
Sbjct: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLY 78

Query: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138
           ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY
Sbjct: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138

Query: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198
           GRYK+HSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM
Sbjct: 139 GRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198

Query: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258
           GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM
Sbjct: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258

Query: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318
           VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL
Sbjct: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318

Query: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378
           EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN+I
Sbjct: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDI 378

Query: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438
           ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA
Sbjct: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438

Query: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498
           PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA
Sbjct: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498

Query: 499 MLKEKTS 505
           MLKE+TS
Sbjct: 499 MLKEETS 505

BLAST of Cp4.1LG01g06560 vs. NCBI nr
Match: XP_022942566.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 480/487 (98.56%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78
           ALQSSF+HSSQ AVLEVCS+EQEVVIALGSNVGDRLQNFN ALQLMKKAGIHITRHA LY
Sbjct: 19  ALQSSFVHSSQGAVLEVCSQEQEVVIALGSNVGDRLQNFNEALQLMKKAGIHITRHAGLY 78

Query: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138
           ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY
Sbjct: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138

Query: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198
           GRYK+HSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACW+SLAADRGGLFELWEKM
Sbjct: 139 GRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWNSLAADRGGLFELWEKM 198

Query: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258
           GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM
Sbjct: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258

Query: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318
           VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL
Sbjct: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318

Query: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378
           EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI
Sbjct: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378

Query: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438
           ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA
Sbjct: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438

Query: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498
           PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA
Sbjct: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498

Query: 499 MLKEKTS 505
           MLKEKTS
Sbjct: 499 MLKEKTS 505

BLAST of Cp4.1LG01g06560 vs. NCBI nr
Match: KAG6599873.1 (Folate synthesis bifunctional protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 450/454 (99.12%), Postives = 453/454 (99.78%), Query Frame = 0

Query: 52  DRLQNFNGALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAV 111
           DRLQNFN ALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAV
Sbjct: 18  DRLQNFNEALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAV 77

Query: 112 KNIEKQLGRTAGIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGS 171
           KNIEKQLGRTAGIRYGPRPIDLDILLYGRYK+HSDTLTVPHERIWERPFVLAPLIDLLGS
Sbjct: 78  KNIEKQLGRTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGS 137

Query: 172 DVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGV 231
           DVDTDDVACW+SLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGV
Sbjct: 138 DVDTDDVACWNSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGV 197

Query: 232 LNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVP 291
           LNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVP
Sbjct: 198 LNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVP 257

Query: 292 VLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPY 351
           VLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPY
Sbjct: 258 VLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPY 317

Query: 352 IAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNT 411
           IAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNT
Sbjct: 318 IAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNT 377

Query: 412 KQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVT 471
           KQNLEILGG+PKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVT
Sbjct: 378 KQNLEILGGIPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVT 437

Query: 472 IGVLGGANIARVHNVRDNVDAVRLCDAMLKEKTS 505
           IGVLGGANIARVHNVRDNVDAVRLCDAMLKEKTS
Sbjct: 438 IGVLGGANIARVHNVRDNVDAVRLCDAMLKEKTS 471

BLAST of Cp4.1LG01g06560 vs. NCBI nr
Match: XP_008456884.1 (PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis melo] >XP_016902055.1 PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis melo])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 439/488 (89.96%), Postives = 461/488 (94.47%), Query Frame = 0

Query: 19  ALQSSFIHSS-QDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACL 78
           ALQ SF HSS QD V+E+CSREQEVVIALGSNVGDRLQNFN AL+LMKKAGIHITRHACL
Sbjct: 19  ALQISFFHSSSQDKVVEICSREQEVVIALGSNVGDRLQNFNEALRLMKKAGIHITRHACL 78

Query: 79  YETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138
           YETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL
Sbjct: 79  YETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138

Query: 139 YGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEK 198
           YGRYKIHSD LT+PHERIWERPFVLAPLIDLLGSDVDTDDVA WHSLAAD GGLFE WEK
Sbjct: 139 YGRYKIHSDILTIPHERIWERPFVLAPLIDLLGSDVDTDDVASWHSLAADHGGLFESWEK 198

Query: 199 MGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRS 258
           +GGE L+GKEGMRRVL +GN+LWDWSCKTS+MGVLNLTPDSFSDGGKFQ IEAAVSQVRS
Sbjct: 199 VGGEYLVGKEGMRRVLSVGNSLWDWSCKTSVMGVLNLTPDSFSDGGKFQSIEAAVSQVRS 258

Query: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVA 318
           MVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT MPEM GKLISVDTFYS+VA
Sbjct: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRMPEMGGKLISVDTFYSKVA 318

Query: 319 LEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNE 378
           LEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGDPSTMQNNENLQYDDVCN+
Sbjct: 319 LEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGDPSTMQNNENLQYDDVCNQ 378

Query: 379 IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSH 438
           IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL GVPKIR  IARRSLGLSH
Sbjct: 379 IALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILTGVPKIRTAIARRSLGLSH 438

Query: 439 APMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCD 498
           APMLIGPSRKKFLGEVCS+ +AT+RDPATVAAVT+GVLGGANI RVHNVRDNVDAVRLCD
Sbjct: 439 APMLIGPSRKKFLGEVCSRSVATERDPATVAAVTVGVLGGANIVRVHNVRDNVDAVRLCD 498

Query: 499 AMLKEKTS 505
           AM KEK S
Sbjct: 499 AMQKEKKS 506

BLAST of Cp4.1LG01g06560 vs. ExPASy TrEMBL
Match: A0A6J1JQH6 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111487416 PE=3 SV=1)

HSP 1 Score: 966 bits (2497), Expect = 0.0
Identity = 482/487 (98.97%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78
           ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGAL+L+KKAGIHITRHACLY
Sbjct: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLY 78

Query: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138
           ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY
Sbjct: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138

Query: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198
           GRYK+HSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM
Sbjct: 139 GRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198

Query: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258
           GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM
Sbjct: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258

Query: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318
           VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL
Sbjct: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318

Query: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378
           EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN+I
Sbjct: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDI 378

Query: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438
           ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA
Sbjct: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438

Query: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498
           PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA
Sbjct: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498

Query: 499 MLKEKTS 505
           MLKE+TS
Sbjct: 499 MLKEETS 505

BLAST of Cp4.1LG01g06560 vs. ExPASy TrEMBL
Match: A0A6J1FP77 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111447567 PE=3 SV=1)

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 480/487 (98.56%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 19  ALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLY 78
           ALQSSF+HSSQ AVLEVCS+EQEVVIALGSNVGDRLQNFN ALQLMKKAGIHITRHA LY
Sbjct: 19  ALQSSFVHSSQGAVLEVCSQEQEVVIALGSNVGDRLQNFNEALQLMKKAGIHITRHAGLY 78

Query: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138
           ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY
Sbjct: 79  ETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLY 138

Query: 139 GRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKM 198
           GRYK+HSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACW+SLAADRGGLFELWEKM
Sbjct: 139 GRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWNSLAADRGGLFELWEKM 198

Query: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258
           GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM
Sbjct: 199 GGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSM 258

Query: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318
           VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL
Sbjct: 259 VSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVAL 318

Query: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378
           EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI
Sbjct: 319 EAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEI 378

Query: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438
           ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA
Sbjct: 379 ASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHA 438

Query: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498
           PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA
Sbjct: 439 PMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDA 498

Query: 499 MLKEKTS 505
           MLKEKTS
Sbjct: 499 MLKEKTS 505

BLAST of Cp4.1LG01g06560 vs. ExPASy TrEMBL
Match: A0A1S4E253 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103496695 PE=3 SV=1)

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 439/488 (89.96%), Postives = 461/488 (94.47%), Query Frame = 0

Query: 19  ALQSSFIHSS-QDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACL 78
           ALQ SF HSS QD V+E+CSREQEVVIALGSNVGDRLQNFN AL+LMKKAGIHITRHACL
Sbjct: 19  ALQISFFHSSSQDKVVEICSREQEVVIALGSNVGDRLQNFNEALRLMKKAGIHITRHACL 78

Query: 79  YETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138
           YETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL
Sbjct: 79  YETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138

Query: 139 YGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEK 198
           YGRYKIHSD LT+PHERIWERPFVLAPLIDLLGSDVDTDDVA WHSLAAD GGLFE WEK
Sbjct: 139 YGRYKIHSDILTIPHERIWERPFVLAPLIDLLGSDVDTDDVASWHSLAADHGGLFESWEK 198

Query: 199 MGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRS 258
           +GGE L+GKEGMRRVL +GN+LWDWSCKTS+MGVLNLTPDSFSDGGKFQ IEAAVSQVRS
Sbjct: 199 VGGEYLVGKEGMRRVLSVGNSLWDWSCKTSVMGVLNLTPDSFSDGGKFQSIEAAVSQVRS 258

Query: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVA 318
           MVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT MPEM GKLISVDTFYS+VA
Sbjct: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRMPEMGGKLISVDTFYSKVA 318

Query: 319 LEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNE 378
           LEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGDPSTMQNNENLQYDDVCN+
Sbjct: 319 LEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGDPSTMQNNENLQYDDVCNQ 378

Query: 379 IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSH 438
           IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL GVPKIR  IARRSLGLSH
Sbjct: 379 IALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILTGVPKIRTAIARRSLGLSH 438

Query: 439 APMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCD 498
           APMLIGPSRKKFLGEVCS+ +AT+RDPATVAAVT+GVLGGANI RVHNVRDNVDAVRLCD
Sbjct: 439 APMLIGPSRKKFLGEVCSRSVATERDPATVAAVTVGVLGGANIVRVHNVRDNVDAVRLCD 498

Query: 499 AMLKEKTS 505
           AM KEK S
Sbjct: 499 AMQKEKKS 506

BLAST of Cp4.1LG01g06560 vs. ExPASy TrEMBL
Match: A0A0A0KP07 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_5G190480 PE=3 SV=1)

HSP 1 Score: 883 bits (2282), Expect = 0.0
Identity = 436/488 (89.34%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 19  ALQSSFIHSS-QDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACL 78
           ALQ SF+HSS QD V+E+CS+EQEVVIALGSNVGDRLQNFN AL+LMKKAGIHITRHACL
Sbjct: 19  ALQISFLHSSSQDKVVEICSQEQEVVIALGSNVGDRLQNFNEALRLMKKAGIHITRHACL 78

Query: 79  YETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138
           YETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL
Sbjct: 79  YETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILL 138

Query: 139 YGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEK 198
           YGRYK+HSDTLT+PHERIWERPFVLAPLIDLLGSDVDTDDVA WHSLAAD GGLFE WEK
Sbjct: 139 YGRYKVHSDTLTIPHERIWERPFVLAPLIDLLGSDVDTDDVASWHSLAADHGGLFESWEK 198

Query: 199 MGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRS 258
           +GGE L+GKEGMRRVL IGN+LWDWSCKTS+MGVLNLTPDSFSDGGKFQ IEAAVSQVRS
Sbjct: 199 VGGEYLVGKEGMRRVLSIGNSLWDWSCKTSVMGVLNLTPDSFSDGGKFQSIEAAVSQVRS 258

Query: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVA 318
           MVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT MPEMSGKLISVDTFYS+VA
Sbjct: 259 MVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRMPEMSGKLISVDTFYSKVA 318

Query: 319 LEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNE 378
           LEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGDPSTMQN ENLQYDDVCN+
Sbjct: 319 LEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGDPSTMQNKENLQYDDVCNQ 378

Query: 379 IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSH 438
           IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL G+PKIRA IA+RSLGLSH
Sbjct: 379 IALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILTGIPKIRAAIAKRSLGLSH 438

Query: 439 APMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCD 498
           APMLIGPSRKKFLGEVCS+ +AT+RDPAT+AAVT+GVLGGANI RVHNVR+NVDAVRLCD
Sbjct: 439 APMLIGPSRKKFLGEVCSRSVATERDPATIAAVTVGVLGGANIVRVHNVRNNVDAVRLCD 498

Query: 499 AMLKEKTS 505
           AM KEK S
Sbjct: 499 AMQKEKKS 506

BLAST of Cp4.1LG01g06560 vs. ExPASy TrEMBL
Match: A0A6J1DAU0 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111018761 PE=3 SV=1)

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 432/487 (88.71%), Postives = 455/487 (93.43%), Query Frame = 0

Query: 17  IPALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHAC 76
           I A Q SFIHS QD +LEVCS+EQEVVIALGSNVGDRL NFN AL+LMK+ GIHITRHAC
Sbjct: 6   ISASQISFIHSLQDPLLEVCSQEQEVVIALGSNVGDRLYNFNEALKLMKREGIHITRHAC 65

Query: 77  LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDIL 136
           LYETAPAYVT+QPQFLNSAVRA TKLGPHELLS VK IEK+LGRTAGIRYGPRPIDLDIL
Sbjct: 66  LYETAPAYVTDQPQFLNSAVRAFTKLGPHELLSTVKRIEKELGRTAGIRYGPRPIDLDIL 125

Query: 137 LYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWE 196
           LYGRY++HSD LTVPHERIWERPFVLAPLIDLLGSDVDTD V+ WHSLA D GGLFE WE
Sbjct: 126 LYGRYRVHSDVLTVPHERIWERPFVLAPLIDLLGSDVDTDVVSGWHSLAPDHGGLFESWE 185

Query: 197 KMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVR 256
           K+GGESLIGKEGMRRVLPIGN+LWDWSCKTSIMG+LNLTPDSFSDGGKFQ +EAAVSQVR
Sbjct: 186 KLGGESLIGKEGMRRVLPIGNSLWDWSCKTSIMGILNLTPDSFSDGGKFQSVEAAVSQVR 245

Query: 257 SMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 316
           SMVSDGADMIDIGAQSTRPMAP ISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV
Sbjct: 246 SMVSDGADMIDIGAQSTRPMAPRISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 305

Query: 317 ALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN 376
           ALEAVK+GAHIVNDVSAGQLDPQMH VV+ L VPYIAMHMRGDP TMQN +NLQYDDVCN
Sbjct: 306 ALEAVKKGAHIVNDVSAGQLDPQMHSVVSDLDVPYIAMHMRGDPYTMQNTQNLQYDDVCN 365

Query: 377 EIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLS 436
           +IASELH RIRDAE SGIPAWRIIVDPGIGFSKNTKQNLEIL G+PKIRAEIARRSLGLS
Sbjct: 366 QIASELHLRIRDAESSGIPAWRIIVDPGIGFSKNTKQNLEILTGLPKIRAEIARRSLGLS 425

Query: 437 HAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLC 496
           HAPML GPSRKKFLGEVCS+ +AT+RDPATVAA+TIGVLGGANI RVHNVRDN DAV LC
Sbjct: 426 HAPMLFGPSRKKFLGEVCSRSVATERDPATVAAITIGVLGGANIVRVHNVRDNADAVSLC 485

Query: 497 DAMLKEK 503
           DAM+KEK
Sbjct: 486 DAMVKEK 492

BLAST of Cp4.1LG01g06560 vs. TAIR 10
Match: AT4G30000.1 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 703.4 bits (1814), Expect = 1.4e-202
Identity = 336/489 (68.71%), Postives = 411/489 (84.05%), Query Frame = 0

Query: 18  PAL-QSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHAC 77
           PAL  S+F  S+    +EV S E EVVIALGSN+G+R+ NF  AL+LMK+ GI +TRH+C
Sbjct: 64  PALCNSAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSC 123

Query: 78  LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDIL 137
           LYETAP +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL
Sbjct: 124 LYETAPVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDIL 183

Query: 138 LYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWE 197
            YG+ +I SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE
Sbjct: 184 FYGKMRISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWE 243

Query: 198 KMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVR 257
           ++GGESLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VR
Sbjct: 244 RLGGESLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVR 303

Query: 258 SMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 317
           SM+S+GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEV
Sbjct: 304 SMISEGADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEV 363

Query: 318 ALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN 377
           A EA+  GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC 
Sbjct: 364 ASEAISNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCK 423

Query: 378 EIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLS 437
           ++ASEL+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +S
Sbjct: 424 DVASELYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVS 483

Query: 438 HAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLC 497
           HAP+L+GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++C
Sbjct: 484 HAPILVGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKVC 543

Query: 498 DAMLKEKTS 506
           DAML+ + S
Sbjct: 544 DAMLRRRRS 552

BLAST of Cp4.1LG01g06560 vs. TAIR 10
Match: AT4G30000.2 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 690.6 bits (1781), Expect = 9.1e-199
Identity = 331/483 (68.53%), Postives = 405/483 (83.85%), Query Frame = 0

Query: 18  PAL-QSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHAC 77
           PAL  S+F  S+    +EV S E EVVIALGSN+G+R+ NF  AL+LMK+ GI +TRH+C
Sbjct: 64  PALCNSAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSC 123

Query: 78  LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDIL 137
           LYETAP +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL
Sbjct: 124 LYETAPVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDIL 183

Query: 138 LYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWE 197
            YG+ +I SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE
Sbjct: 184 FYGKMRISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWE 243

Query: 198 KMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVR 257
           ++GGESLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VR
Sbjct: 244 RLGGESLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVR 303

Query: 258 SMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEV 317
           SM+S+GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEV
Sbjct: 304 SMISEGADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEV 363

Query: 318 ALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCN 377
           A EA+  GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC 
Sbjct: 364 ASEAISNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCK 423

Query: 378 EIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLS 437
           ++ASEL+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +S
Sbjct: 424 DVASELYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVS 483

Query: 438 HAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLC 497
           HAP+L+GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++ 
Sbjct: 484 HAPILVGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKID 543

Query: 498 DAM 500
            A+
Sbjct: 544 TAV 546

BLAST of Cp4.1LG01g06560 vs. TAIR 10
Match: AT1G69190.1 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 660.6 bits (1703), Expect = 1.0e-189
Identity = 313/468 (66.88%), Postives = 396/468 (84.62%), Query Frame = 0

Query: 40  QEVVIALGSNVGDRLQNFNGALQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAV 99
           +EVVIALGSNVG+R+ NF  AL+LMK  GI +TRH+CLYET P +VT+QP+FLN+A+R V
Sbjct: 12  EEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGV 71

Query: 100 TKLGPHELLSAVKNIEKQLGRTA-GIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWER 159
           TKL PHELL+ +K IEK++GR   G+RYGPRP+DLDIL YG++KI SD L +PHERIWER
Sbjct: 72  TKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWER 131

Query: 160 PFVLAPLIDLLGS-DVDTDD-VACWHSLAADRGGLFELWEKMGGESLIGKEG-MRRVLPI 219
           PFVLAPL+DLLG+ D+D D  VA WHSL+   GG+F+ WE++GGESL+GK+G ++RV+PI
Sbjct: 132 PFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPI 191

Query: 220 GNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRP 279
           G++LWD+S KT +MG+LNLTPDSFSDGGKFQ ++ AVS+VRSM+S+G D+IDIGAQSTRP
Sbjct: 192 GDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRP 251

Query: 280 MAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQ 339
           MA  IS +EE+DRL+PVL+ V GM EM GKLISVDTF SEVALEA++ GA I+NDVS G 
Sbjct: 252 MASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGS 311

Query: 340 LDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNEIASELHSRIRDAELSGIP 399
           LD  MH+VVA   VPY+ MHMRGDP TMQN ENL+Y+++C ++A+EL+ R+R+AELSGIP
Sbjct: 312 LDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIP 371

Query: 400 AWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCS 459
           AWRI++DPGIGFSK    NL+I+  +PKIR E+A++S+GLSHAP+LIGPSRK+FLG++C 
Sbjct: 372 AWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICG 431

Query: 460 QPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLKEK 504
           +P A++RD ATVA VT G+L GANI RVHNVRDNVDA RLCDAM+ ++
Sbjct: 432 RPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMMTKR 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048623.6e-20870.35Folate synthesis bifunctional protein, mitochondrial OS=Pisum sativum OX=3888 GN... [more]
Q9SZV31.9e-20168.71Folate synthesis bifunctional protein, mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q1ENB61.4e-18866.88Folate synthesis bifunctional protein OS=Arabidopsis thaliana OX=3702 GN=CytHPPK... [more]
P292512.1e-8338.80Folic acid synthesis protein fol1 OS=Pneumocystis carinii OX=4754 GN=fol1 PE=1 S... [more]
Q4LB351.1e-7137.91Folic acid synthesis protein fol1 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
XP_023536333.10.0100.00folate synthesis bifunctional protein, mitochondrial-like [Cucurbita pepo subsp.... [more]
XP_022990585.10.098.97folate synthesis bifunctional protein, mitochondrial-like [Cucurbita maxima][more]
XP_022942566.10.098.56folate synthesis bifunctional protein, mitochondrial-like [Cucurbita moschata][more]
KAG6599873.10.099.12Folate synthesis bifunctional protein, mitochondrial, partial [Cucurbita argyros... [more]
XP_008456884.10.089.96PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis me... [more]
Match NameE-valueIdentityDescription
A0A6J1JQH60.098.976-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1FP770.098.566-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita moschata OX=366... [more]
A0A1S4E2530.089.966-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0KP070.089.346-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis sativus OX=3659 G... [more]
A0A6J1DAU00.088.716-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT4G30000.11.4e-20268.71Dihydropterin pyrophosphokinase / Dihydropteroate synthase [more]
AT4G30000.29.1e-19968.53Dihydropterin pyrophosphokinase / Dihydropteroate synthase [more]
AT1G69190.11.0e-18966.88Dihydropterin pyrophosphokinase / Dihydropteroate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0359077,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK superfamilyGENE3D3.30.70.560coord: 39..180
e-value: 1.1E-49
score: 170.7
IPR0359077,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK superfamilySUPERFAMILY550836-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPKcoord: 42..171
IPR000489Pterin-binding domainPFAMPF00809Pterin_bindcoord: 229..484
e-value: 1.8E-84
score: 283.2
IPR000489Pterin-binding domainPROSITEPS00793DHPS_2coord: 262..275
IPR000489Pterin-binding domainPROSITEPS00792DHPS_1coord: 228..243
IPR000489Pterin-binding domainPROSITEPS50972PTERIN_BINDINGcoord: 226..494
score: 73.525932
IPR0005507,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPKTIGRFAMTIGR01498TIGR01498coord: 42..168
e-value: 1.7E-37
score: 126.4
IPR0005507,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPKPFAMPF01288HPPKcoord: 43..168
e-value: 2.7E-33
score: 114.8
IPR0005507,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPKPROSITEPS00794HPPKcoord: 125..136
IPR0005507,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPKCDDcd00483HPPKcoord: 42..168
e-value: 2.26767E-58
score: 187.301
IPR011005Dihydropteroate synthase-likeGENE3D3.20.20.20coord: 207..504
e-value: 4.3E-105
score: 353.0
IPR011005Dihydropteroate synthase-likeSUPERFAMILY51717Dihydropteroate synthetase-likecoord: 215..501
IPR006390Dihydropteroate synthase domainTIGRFAMTIGR01496TIGR01496coord: 228..498
e-value: 3.5E-82
score: 273.9
IPR006390Dihydropteroate synthase domainCDDcd00739DHPScoord: 226..497
e-value: 1.07846E-119
score: 349.983
NoneNo IPR availablePANTHERPTHR20941:SF1FOLIC ACID SYNTHESIS PROTEIN FOL1coord: 32..501
IPR045031Dihydropteroate synthasePANTHERPTHR20941FOLATE SYNTHESIS PROTEINScoord: 32..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g06560.1Cp4.1LG01g06560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046656 folic acid biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0046654 tetrahydrofolate biosynthetic process
biological_process GO:0044237 cellular metabolic process
biological_process GO:0009396 folic acid-containing compound biosynthetic process
biological_process GO:0042558 pteridine-containing compound metabolic process
molecular_function GO:0003848 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004156 dihydropteroate synthase activity
molecular_function GO:0016301 kinase activity