Cp4.1LG01g05880 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g05880
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING finger and transmembrane domain-containing protein
LocationCp4.1LG01: 471537 .. 477280 (-)
RNA-Seq ExpressionCp4.1LG01g05880
SyntenyCp4.1LG01g05880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCACTATTTGCCTCTCTCTACGTTTCGCATTCAATTCTCCATTTTTTTTTTCCCAACCTATTTCTTCGTTTCCTTTTTCCTTCCCCAAATTCCCCCGGCGACCCACCTCCCTCCGCCCCCTTCCGACCGGATCACCGCCATTTCCGGCTCCAAACGCCACATTTATTCTTGCCCATCATCCTCTGTTTTTTTTCCTTTCAGGTGGTGGGATTTCGAAGGGGTTGAATCGGTACAGGTTTTGCAAGGAGGGGGAAGGTCGAACATCATTTTGGGCTTTTGCCTTTTGCCTTTTGGTCACTACCAATGGAGACCTCGGCTTCCAATTCTGATCCTTATTCCAATTCTTCTTCACCCACAACTTCTCCTACCTCCTCCAGATTTGGGGTCTCGATCCCTTCGCTCAATTTCTTCCGTTCGCCTCTGTCCGTCATTTTGGATTACTCTGGAATTCTTCCCACTAGGGCTGTTCCTCATGAATCGGAACCTATAATCAACGGCTCTGCCACCCCTGATTTGCGGGTTTATTCTCAAAATCAGAATCACTTGATGACTACCACTTCTTCTTCTTCTTCTTCTTCTAGTAATAGTCCCACCTCCTCCTCCTCCGGTAATACCAGCCCCTCTGGGGAGGTTTCGATTAGGATTATTGGGTCTGGTGAGCAAGATCCTTCTCCGGCTCTGGTTGGACAAGCTGGCTATGAGCAAAATGGTGGACTTCCTGTTTCTGGGGATCAGCCGGAATCCCCTGATGAGGAACGAGTTCCTTTGGTGTCCACTGCTTCTTCGTCTATTCAATCTGGAAGCAGTCGTGTTTCAGAATCTGGAAATGGCGTGGAAGAGAGTGTTAATAGAGATTCGTCTTATCAGAGATACGATATCCAGCAGGTTGCCAGGTGGATTGAACAGATTCTTCCTTTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTGGAAGGTGCTCTCTTTTGTCCTCTCTTTTGTTGGACCGATTATATTTTTCGATTTTCATTGCCCTTTGCACTTGGCACCACTTTGACGTATTGTTCATTTTCGTTATCGAATTTCATATAGTGCTGGTAGTAACTAGTCCTTGTTAAGATATAATTAAGTGGGAATGCTGGCCATTGTTTACTCAGGACTTCCGTTGTATCTATTAAAATTTCACCCTCGTTAGTTCTTATACCTTTTAGATGACTTACAGCGTGATTGTAGAGGATAATGTTTGATTTGAATACTGGTGTGGTGTGGTAAATTGCCCCGGATGTTTTTTTTTTTTTCCTTTCCGTACTTGAGTACTGAAATTGCATCGCTGTAGCTGTAAAAGCTACTGTAGTATATTGTAGGGTTTCTTGCAAGGCGGTTGTTGTAAGATGTAGTGATAGAATCATCCAAGAATTTGGAGACCAGAGTTTTTTTAAAGCGTTGGAACTATCTTACTATCAAATGCATATAATTTTCAATGAAGAAAAATAACTGGATTAACTTTGTTGACAATTGGGTTCTGCTATTAACACATGTTTTCATGTATTGAAAAATAAATATGCGTTGAAAAGGCAGCTCTCGGGAGTGGGGAGGAAGGTGTTGAGAAAGAATTAGAGGAATACTGTGGAAACAGTTATGTTAGCATCAGATCATTTGGTTTGGAGATGACACTTTGCCATAAATCTTTGGAACATACTTTTGGACACGTTTGTTTGGTAGTCGACATATACATTAATTATCTTCCACTACCTAACCTAATAAGGGAGTTGCATTACACTGTTAGGTGTAATCACAAAGACAAGTAGTGATCATCTTGTAATCTTCTCCCTTTTACTCTCTTGCTGTAACATTCTTCTGATTGGTGAGAATTTATTTGTTTGATATTTCCAGGTTTTTTCATCACAATTTGTATATCAGCTGTTATGTATAAGTCGAACGATATTGTCAAGAAACAGACTGCTCTAAAGGTGGTGTAAGCCTAAACCCAAACTTATCTTATCTATTAGTACCTTATTTCTTTTTCAACTTCACTGAGAATCCACTTGCAAGAAATATACACTGAAGAATTTACTCTCCTTTTCAGGGGGAGAGGAAAATTTCTGTCCTTGCTGGGACATCTATTGCATTTTTGGTTCATGTTATTGGAATTTACTGGTGGTGCCATAGTGATGATCTTCTACACCCGTTAGTCATGCTTCCACCAAAGACAGTACCACCATTTTGGCATGCCTTGTTCATAATCTTAGTTAACGGTACAGATTTTCCCTTGCTTTGGCTTATTAAGTTTCTGTAGATATAATGATTGAATTAACATATGGAATGGAGCTTCAAAAAATGTAAGCAGAAAATTCTTAAGTGTCACAGTGCGATTTCTAATTAGTTAGCTTGAATGGTGATATAACAAGGGCATAAATATGGTTTATTATCTTTGTGTTATCTATATGGATCTTGCAAACATAGTTTTTAAGACAGGATTCAGGATATTGCCAAAAATTGAAAGAATGATATTGTGGTTTTTTCTTTTTCTTTTATTATTATTATTTTTTTAATGGTTCAACCTTCCTCTAATTCCAACTTCTTTGTTGTATTTCTCTCACAATTTTTTGTACAATTTTTCATACACTTGATTTTAATGTTCTCATTCTTTGATTTTGGACTCGATTGTGTTGCCATGCCAGATACGATGGCGAGGCAGGTTGCAATGGCTCTCAAATGTCTTCTTCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGCCGGCAGGTATTATGAGATCTCAAATTTAGCTTAATCATATGTTTTGTGTTTATCTTGTATTAACCATTAATATTATTGAAATGTCTGACAATTACTTTGTTTTAGTTCTTTGATTGTTTATAATTCATGTTTCAGGGTCAAATTTTAACTCTCATGGAATACACTCTTCTGTTGTATCGTGCATTATTACCGGCACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGGAGCCTTTTTTCATCACTGACAACAGGATTGTATTTAACTTTCAAGCTAACGTCTGTTGTTGAGAAGGTATGGTTTAAAACATGAATATTCATACCTTAATAAGCACTAAAAATTTTAATATTATGTCATAGTTCGTGGAAACCAACCTTGTTCATATACTATCTATTTTGTTATTTTCTCTTTAGATTCAATCATTCGTGGCTGCATTTAAGGCTCTATCACAAAAAGAAGTCCATTATGGATCATATGCCACATCAGAACAGGCAAGTATATACATACCTGTCTATTGTAGTCTCTTTCGAAATTTACCATTTTAAAACAGCTTTGTGTTTCTTCTTGCATTTAACTTTACGCATCTGTTTTCACATTCTATTTAATGTATCCCGCAAATAACGAAATAACATGAATATTTGTGGAACCCTTTAAGATGGTTGAATGTCTCCTTTTTTAATGCTTTCTTAATGTTACAAGTCATTCCCTCTATAGTTCATGTTAACGGGATTCCCTCATTACATTCTCATGGATATGTGATACTATTACTAGCCTACAGGATATGGTGGGAGAAGTGACCTAGAATTTTTCTTAGATAAGATAAACCAATTTGTCACTATCTTTCATTGGTGTGGCGACATAACTTTGTACCTTTTGAAGTTACATGATTGACTCTTCTATATGTAACTTTTTTTTTTCTTTCTTTCTCTCTTTCTTTCTTTGCTACTTCCCTTTTATAATTCTGGCTAATTGGATTGATTCATTTTAAATACCTTTTGGTCTTTATTTGGTTGGGTATTGAAATTTTAGTTTTTATGTTAAAAAGCTGCATTTCAATGAAAGTTTTAGGGGGGTACAAGTAAAATTTGCTTTTTTCTTTTATATTTCTGTAGTTACTCGTATCAGGCCGTACATCTTGAGATGCTATTTTTGAGGTGTGCTTTTTTCATTTTTTTGATGCATGAAGAGATAAAAAAAAAAAAAGTATAATCGTGATTTGGCAATTAGCGCCCGTAATTTCCTGCGTGTCACCAACATTGAAGCAGGAAAAGTTCTCGACATTAGTATTGTTATGTACATGATTAGTTGAATATAATCTCTCCTCCCTGATAAATATCTGTCCTGTGATTTCTCTAGCATTGCATGATCTAATAGTAACTTCATGCATTAATTAAATTCCTTCAAGTCTTCCTTATACTTTATTTTACAGGTAAGTGCTGCGGGAGACCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGCTGTAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTCAGTATGATGACTTCACATCTCTTGTGTTTTCTGAATACTACCAGCATTGCATTACTATATTCATATCAGACCTCCATAGGACCAATATATAGATTATAACAGGGAGACAAGCATTCTAATTTGAACTCTTAGGTACTGTAGTAATTATCTACCTTGTTATGAGCCAAAAAGTCATGCTCTTGGCTGAGAAAAACTGAATTTGTTCATCAGGTATCGAGGAATTAGTTCAGTTTATTTTTAAATTCTTTTACTGGATGGCCTATCATATAAAGTAGAGGTATATAATTTTTAGAGTTTTTTTGCAAATCCAAACACATCACGTTATCATTCCATCCTCATATGTATCTTGTGAATCCTTAGGACTTAACCATGCTGCAGTCATTAATATTAGAAATGATCTCTATGGTCATATCATTCTTATTATTAGTGCAGGGGATTGTCTATGTCTGACTAGTATGGCTACAGCATTTATTTGACTGAGACTATGCTGTGTTGCCCACAGTTTTTTTCATTTACTCCTGCGCACGCACACGACAATGGACCTACTTGTGCGCACAGAGATGAAGGAACCAAAAGAAAAAAGAAAATGAAGAAAGGACAGAACCCCTTGAATCTTGATTAATAGTGGCACGATTCTTTCGACTGGGCATAAGTCTAGTGCTTAGGAATTAGTTTTAGCTCTAACAAATGTCATTGCATTTCCATTTACTGGTTCAAGTACTTGTGTTTTCAGGTTTGAAAGAGAAAGAACTTGCCCTCTCTGCCGGGCGCTGGTAAAGCCAGCAGAACTCCGAACATTTGGCGACGGGTCGACCAGCTTACTCTTCCAGTTGTTTTAGACAACCATTTGACATTTCTGGACGTAACAGCTCTATCAGCTTGACAAAGAAACATATTGTTTATTGGATGGACACAGGGGGGAAGAAAAAGAACAAAGAGAAGGGGAAAGAAGAGAAAAAGAAAAAGGGAAACATAACATAACATCCAGATGAGTTTAAAGATGCTGTCAAATTCAAAACTGGATTAGTATTATATTAATGCTTTGTAATCATTGTGACGAGTTGAGTTAGATGTCCAAACAGAAGATGGATTTAATAAAATCCCATTTCATCATTAAATTCAATGAAATCTAATTCTTAACGAAAAGAATCCCACTGTGAAAACTGCATGTTACTTGAAGACTGATGAGTATTCCCCGTAGAGTTTAGGCCTGGAAACAGCTGCCATGTTGAATGTGTTCAGTCTGCAGTGATTATGTAAACACACAAATAATTGTTGTTCATATACTGTTCTACAGGTTTCAAGTGTGAGGCGTCACCATCTTATGACCTGTTCATACTGTTTCTGAGGCCTTTTTTCTGTGCTCTGAACAGTGAAGTTATGAAATCATCAAAATTGAACTATTTGTTGTAATGATCAAAGCCAAGTAGTATATATGCAACCATTTGTT

mRNA sequence

CCTCCACTATTTGCCTCTCTCTACGTTTCGCATTCAATTCTCCATTTTTTTTTTCCCAACCTATTTCTTCGTTTCCTTTTTCCTTCCCCAAATTCCCCCGGCGACCCACCTCCCTCCGCCCCCTTCCGACCGGATCACCGCCATTTCCGGCTCCAAACGCCACATTTATTCTTGCCCATCATCCTCTGTTTTTTTTCCTTTCAGGTGGTGGGATTTCGAAGGGGTTGAATCGGTACAGGTTTTGCAAGGAGGGGGAAGGTCGAACATCATTTTGGGCTTTTGCCTTTTGCCTTTTGGTCACTACCAATGGAGACCTCGGCTTCCAATTCTGATCCTTATTCCAATTCTTCTTCACCCACAACTTCTCCTACCTCCTCCAGATTTGGGGTCTCGATCCCTTCGCTCAATTTCTTCCGTTCGCCTCTGTCCGTCATTTTGGATTACTCTGGAATTCTTCCCACTAGGGCTGTTCCTCATGAATCGGAACCTATAATCAACGGCTCTGCCACCCCTGATTTGCGGGTTTATTCTCAAAATCAGAATCACTTGATGACTACCACTTCTTCTTCTTCTTCTTCTTCTAGTAATAGTCCCACCTCCTCCTCCTCCGGTAATACCAGCCCCTCTGGGGAGGTTTCGATTAGGATTATTGGGTCTGGTGAGCAAGATCCTTCTCCGGCTCTGGTTGGACAAGCTGGCTATGAGCAAAATGGTGGACTTCCTGTTTCTGGGGATCAGCCGGAATCCCCTGATGAGGAACGAGTTCCTTTGGTGTCCACTGCTTCTTCGTCTATTCAATCTGGAAGCAGTCGTGTTTCAGAATCTGGAAATGGCGTGGAAGAGAGTGTTAATAGAGATTCGTCTTATCAGAGATACGATATCCAGCAGGTTGCCAGGTGGATTGAACAGATTCTTCCTTTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTGGAAGGTTTTTTCATCACAATTTGTATATCAGCTGTTATGTATAAGTCGAACGATATTGTCAAGAAACAGACTGCTCTAAAGGGGGAGAGGAAAATTTCTGTCCTTGCTGGGACATCTATTGCATTTTTGGTTCATGTTATTGGAATTTACTGGTGGTGCCATAGTGATGATCTTCTACACCCGTTAGTCATGCTTCCACCAAAGACAGTACCACCATTTTGGCATGCCTTGTTCATAATCTTAGTTAACGATACGATGGCGAGGCAGGTTGCAATGGCTCTCAAATGTCTTCTTCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGCCGGCAGGGTCAAATTTTAACTCTCATGGAATACACTCTTCTGTTGTATCGTGCATTATTACCGGCACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGGAGCCTTTTTTCATCACTGACAACAGGATTGTATTTAACTTTCAAGCTAACGTCTGTTGTTGAGAAGATTCAATCATTCGTGGCTGCATTTAAGGCTCTATCACAAAAAGAAGTCCATTATGGATCATATGCCACATCAGAACAGGCAAGTATATACATACCTGTAAGTGCTGCGGGAGACCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGCTGTAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTTTGAAAGAGAAAGAACTTGCCCTCTCTGCCGGGCGCTGGTAAAGCCAGCAGAACTCCGAACATTTGGCGACGGGTCGACCAGCTTACTCTTCCAGTTGTTTTAGACAACCATTTGACATTTCTGGACGTAACAGCTCTATCAGCTTGACAAAGAAACATATTGTTTATTGGATGGACACAGGGGGGAAGAAAAAGAACAAAGAGAAGGGGAAAGAAGAGAAAAAGAAAAAGGGAAACATAACATAACATCCAGATGAGTTTAAAGATGCTGTCAAATTCAAAACTGGATTAGTATTATATTAATGCTTTGTAATCATTGTGACGAGTTGAGTTAGATGTCCAAACAGAAGATGGATTTAATAAAATCCCATTTCATCATTAAATTCAATGAAATCTAATTCTTAACGAAAAGAATCCCACTGTGAAAACTGCATGTTACTTGAAGACTGATGAGTATTCCCCGTAGAGTTTAGGCCTGGAAACAGCTGCCATGTTGAATGTGTTCAGTCTGCAGTGATTATGTAAACACACAAATAATTGTTGTTCATATACTGTTCTACAGGTTTCAAGTGTGAGGCGTCACCATCTTATGACCTGTTCATACTGTTTCTGAGGCCTTTTTTCTGTGCTCTGAACAGTGAAGTTATGAAATCATCAAAATTGAACTATTTGTTGTAATGATCAAAGCCAAGTAGTATATATGCAACCATTTGTT

Coding sequence (CDS)

ATGGAGACCTCGGCTTCCAATTCTGATCCTTATTCCAATTCTTCTTCACCCACAACTTCTCCTACCTCCTCCAGATTTGGGGTCTCGATCCCTTCGCTCAATTTCTTCCGTTCGCCTCTGTCCGTCATTTTGGATTACTCTGGAATTCTTCCCACTAGGGCTGTTCCTCATGAATCGGAACCTATAATCAACGGCTCTGCCACCCCTGATTTGCGGGTTTATTCTCAAAATCAGAATCACTTGATGACTACCACTTCTTCTTCTTCTTCTTCTTCTAGTAATAGTCCCACCTCCTCCTCCTCCGGTAATACCAGCCCCTCTGGGGAGGTTTCGATTAGGATTATTGGGTCTGGTGAGCAAGATCCTTCTCCGGCTCTGGTTGGACAAGCTGGCTATGAGCAAAATGGTGGACTTCCTGTTTCTGGGGATCAGCCGGAATCCCCTGATGAGGAACGAGTTCCTTTGGTGTCCACTGCTTCTTCGTCTATTCAATCTGGAAGCAGTCGTGTTTCAGAATCTGGAAATGGCGTGGAAGAGAGTGTTAATAGAGATTCGTCTTATCAGAGATACGATATCCAGCAGGTTGCCAGGTGGATTGAACAGATTCTTCCTTTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTGGAAGGTTTTTTCATCACAATTTGTATATCAGCTGTTATGTATAAGTCGAACGATATTGTCAAGAAACAGACTGCTCTAAAGGGGGAGAGGAAAATTTCTGTCCTTGCTGGGACATCTATTGCATTTTTGGTTCATGTTATTGGAATTTACTGGTGGTGCCATAGTGATGATCTTCTACACCCGTTAGTCATGCTTCCACCAAAGACAGTACCACCATTTTGGCATGCCTTGTTCATAATCTTAGTTAACGATACGATGGCGAGGCAGGTTGCAATGGCTCTCAAATGTCTTCTTCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGCCGGCAGGGTCAAATTTTAACTCTCATGGAATACACTCTTCTGTTGTATCGTGCATTATTACCGGCACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGGAGCCTTTTTTCATCACTGACAACAGGATTGTATTTAACTTTCAAGCTAACGTCTGTTGTTGAGAAGATTCAATCATTCGTGGCTGCATTTAAGGCTCTATCACAAAAAGAAGTCCATTATGGATCATATGCCACATCAGAACAGGCAAGTATATACATACCTGTAAGTGCTGCGGGAGACCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGCTGTAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTTTGAAAGAGAAAGAACTTGCCCTCTCTGCCGGGCGCTGGTAAAGCCAGCAGAACTCCGAACATTTGGCGACGGGTCGACCAGCTTACTCTTCCAGTTGTTTTAG

Protein sequence

METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESEPIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQLF
Homology
BLAST of Cp4.1LG01g05880 vs. ExPASy Swiss-Prot
Match: Q96EX2 (RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RNFT2 PE=2 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 2.5e-33
Identity = 91/301 (30.23%), Postives = 164/301 (54.49%), Query Frame = 0

Query: 191 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKKQTALKGERKI 250
           +++ V  W+++ LPF L+LL     QH  G  + I +++    +N  +++Q +LK +R +
Sbjct: 155 ELKAVICWLQKGLPFILILLAKLCFQHKLGIAVCIGMASTFAYANSTLREQVSLKEKRSV 214

Query: 251 SVLAGTSIAFLV-HVIGIYWWCHSDDLLHPLVMLPPK-TVPPFWHALFIILVNDTMARQV 310
            V+    +AFL  + + + +   S  L + L+ L P   +  F+  L+I+ + D + + +
Sbjct: 215 LVILWI-LAFLAGNTLYVLYTFSSQQLYNSLIFLKPNLEMLDFFDLLWIVGIADFVLKYI 274

Query: 311 AMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLNKDQGSLF- 370
            +ALKCL++   K       + +G+   ++E    L+R+L+P  +WY++ +  D  + + 
Sbjct: 275 TIALKCLIVALPKIILA--VKSKGKFYLVIEELSQLFRSLVPIQLWYKYIMGDDSSNSYF 334

Query: 371 -SSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVSAAGDLC 430
              +   LY   K   +  ++     A K L   + +YG  AT +Q       + AGD+C
Sbjct: 335 LGGVLIVLYSLCKSFDICGRVGGVRKALKLLCTSQ-NYGVRATGQQC------TEAGDIC 394

Query: 431 AICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQL 488
           AICQ +   P++L C+H+FCE+C+  W +RERTCPLCR+ V    LR + DG+TS  FQ+
Sbjct: 395 AICQAEFREPLILLCQHVFCEECLCLWLDRERTCPLCRS-VAVDTLRCWKDGATSAHFQV 444

BLAST of Cp4.1LG01g05880 vs. ExPASy Swiss-Prot
Match: Q5RAG4 (RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=9601 GN=RNFT2 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.8e-32
Identity = 90/301 (29.90%), Postives = 162/301 (53.82%), Query Frame = 0

Query: 191 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKKQTALKGERKI 250
           +++ V  W+++ LPF L+LL     QH  G  + I +++    +N  +++Q +LK +R +
Sbjct: 155 ELKAVICWLQKGLPFILILLAKLCFQHKLGIAVCIGMASTFAYANSTLREQVSLKEKRSV 214

Query: 251 SVLAGTSIAFLV-HVIGIYWWCHSDDLLHPLVMLPPK-TVPPFWHALFIILVNDTMARQV 310
            V+    +AFL  + + + +   S  L + L+ L P      F+  L+I+ + D + + +
Sbjct: 215 LVILWI-LAFLAGNTLYVLYTFSSQQLYNSLIFLKPNLETLDFFDLLWIVGIADFVLKYI 274

Query: 311 AMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLNKDQGSLF- 370
            +ALKCL++   K       + +G+   ++E    L+R+L+P  +WY++ +  D  + + 
Sbjct: 275 TIALKCLIVALPKIILA--VKSKGKFYLVIEELSQLFRSLVPIQLWYKYIMGDDSSNSYF 334

Query: 371 -SSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVSAAGDLC 430
              +   LY   K   +  ++     A K L   + +YG  AT +Q       + AGD+C
Sbjct: 335 LGGVLIVLYSLCKSFDICGRVGGVRKALKLLCTSQ-NYGVRATGQQC------TEAGDIC 394

Query: 431 AICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQL 488
           AICQ +   P++L C+H+FCE+C+  W +RERTCPL R+ V    LR + DG+TS  FQ+
Sbjct: 395 AICQAEFREPLILLCQHVFCEECLCLWLDRERTCPLSRS-VAVDTLRCWKDGATSAHFQV 444

BLAST of Cp4.1LG01g05880 vs. ExPASy Swiss-Prot
Match: Q9DCN7 (E3 ubiquitin-protein ligase RNFT1 OS=Mus musculus OX=10090 GN=Rnft1 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.5e-30
Identity = 87/304 (28.62%), Postives = 150/304 (49.34%), Query Frame = 0

Query: 191 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKKQTALKGERKI 250
           + + + +W+++ LP+ L+L I  + QH+ G  + I +      +N  +  Q  L+ ER  
Sbjct: 103 EFRYLFKWLQKSLPYILILGIKLVMQHITGISLGIGLLTTFMYANKSIVNQVFLR-ERSS 162

Query: 251 SVLAGTSIAFLV-HVIGIYWWCHSDDLLHPLVMLPPKTVP-PFWHALFIILVNDTMARQV 310
            +     + FL    + +Y+  HS  L + L+ L P      FW  L+I+ + D + +  
Sbjct: 163 KLRCAWLLVFLAGSSVLLYYTFHSQSLHYSLIFLNPTLEQLSFWEVLWIVGITDFILKFF 222

Query: 311 AMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLNKDQGSLFS 370
            M LKCL+L+         F+ +G    L+E     YR  +P PVW+R+ ++  +    +
Sbjct: 223 FMGLKCLILLV--PSFIMPFKSKGYWYMLLEELCQYYRIFVPIPVWFRYLISYGEFGNVT 282

Query: 371 SLTTG-----LYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVSAAG 430
           + + G     LYL  KL      +++F    +    +   YG  A+  Q       S   
Sbjct: 283 TWSLGILLALLYLILKLLDFFGHLRTFRQVLRVFFTRP-SYGVPASKRQC------SDMD 342

Query: 431 DLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLL 488
            +C ICQ +   P+LL C+HIFCE+C++ WF RE+TCPLCR ++    +  + DG+TS  
Sbjct: 343 GICTICQAEFQKPVLLFCQHIFCEECITLWFNREKTCPLCRTVISEC-INKWKDGATSSH 395

BLAST of Cp4.1LG01g05880 vs. ExPASy Swiss-Prot
Match: Q3UF64 (RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rnft2 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 2.6e-30
Identity = 94/326 (28.83%), Postives = 167/326 (51.23%), Query Frame = 0

Query: 166 GSSRVSESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI 225
           G      S  GV+E     +     +++ V  W+++ LPF L+LL     QH  G  + I
Sbjct: 132 GGDHRGHSEEGVDEQPGTPAP-ALSELKAVISWLQKGLPFILILLAKLCFQHKLGIAVCI 191

Query: 226 CISAVMYKSNDIVKKQTALKGERKISVLAGTSIAFLV-HVIGIYWWCHSDDLLHPLVMLP 285
            +++    +N  +++Q +LK +R + V+    +AFL  + + + +   S  L   L+ L 
Sbjct: 192 GMASTFAYANSTLREQVSLKEKRSVLVILWI-LAFLAGNTMYVLYTFSSQQLYSSLIFLK 251

Query: 286 PK-TVPPFWHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLL 345
           P      F+  L+I+ + D + + + +ALKCL++   K       + +G+   ++E    
Sbjct: 252 PNLETLDFFDLLWIVGIADFVLKYITIALKCLIVALPKIILA--VKSKGKFYLVIEELSQ 311

Query: 346 LYRALLPAPVWYRFFLNKDQGSLF--SSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKE 405
           L+R+L+P  +WY++ +  D  + +    +   LY   K   +  ++     A K L   +
Sbjct: 312 LFRSLVPIQLWYKYIMGDDSSNSYFLGGVLIVLYSLCKSFDICGRVGGLRKALKLLCTSQ 371

Query: 406 VHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCP 465
            +YG  AT +Q       + AG +CAICQ +   P++L C+H+FCE+C+  W +RERTCP
Sbjct: 372 -NYGVRATGQQC------TEAGAVCAICQAEFRDPMILLCQHVFCEECLCLWLDRERTCP 431

Query: 466 LCRALVKPAELRTFGDGSTSLLFQLF 488
           LCR+ V    LR + DG+TS   Q++
Sbjct: 432 LCRS-VAVDTLRCWKDGATSAHLQVY 445

BLAST of Cp4.1LG01g05880 vs. ExPASy Swiss-Prot
Match: Q6NZ21 (E3 ubiquitin-protein ligase RNFT1 OS=Danio rerio OX=7955 GN=rnft1 PE=2 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 5.8e-30
Identity = 90/329 (27.36%), Postives = 162/329 (49.24%), Query Frame = 0

Query: 166 GSSRVSESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI 225
           G S   ES +G  +  + +SS    +++ + RW+++ LPF ++L    + QH  G  + +
Sbjct: 102 GHSHEHESDSGESDLESGESSSSISELRYLLRWLKKSLPFIVILCAKLVIQHALGLAVAV 161

Query: 226 CISAVMYKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPP 285
            +       N  ++ Q  L  +R+ ++     + FL     + ++      L+  +    
Sbjct: 162 GLFTTFMYVNKSIQTQVFLH-DRRTNLHCAWLLLFLTSSSLLVFYTFHTQSLYRCLFFAN 221

Query: 286 KTVP--PFWHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLL 345
            T+    FW  L+ + V + + + + M  KCL+L+         +RR+GQ   L+E    
Sbjct: 222 ATIDYHNFWEVLWSVGVTNFILKFIFMGFKCLILLV--PCPLMTYRRRGQWYMLIEEVGQ 281

Query: 346 LYRALLPAPVWYRFFLNKDQGSLFSSLTTG-----LYLTFKLTSVVEKIQSFVAAFKALS 405
           LY+ + P P+W+R+ ++ D+      LT G     LYL  KL ++     S     +   
Sbjct: 282 LYQVIAPVPLWFRYLVSYDEMDTSVGLTLGILLALLYLIMKLLALYGLSGSLQKTLRTFF 341

Query: 406 QKEVHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERER 465
             EV+ G+ A+  Q      +  AGD+C ICQ     P +L C+HIFCE+C+++W  +ER
Sbjct: 342 SPEVN-GAPASPAQ------IREAGDICPICQADFKQPRVLVCQHIFCEECIAQWLNQER 401

Query: 466 TCPLCRALVKPAELRTFGDGSTSLLFQLF 488
           TCPLCR ++   ++  + DG+TS   Q++
Sbjct: 402 TCPLCRTVITD-KVHKWKDGATSAHLQIY 419

BLAST of Cp4.1LG01g05880 vs. NCBI nr
Match: XP_023536798.1 (E3 ubiquitin-protein ligase RNFT1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 481/487 (98.77%), Postives = 481/487 (98.77%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120
           PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120

Query: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180
           DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES
Sbjct: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180

Query: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240
           VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK
Sbjct: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240

Query: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILV 300
           QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILV
Sbjct: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILV 300

Query: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLN 360
           NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLN
Sbjct: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLN 360

Query: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVS 420
           KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      VS
Sbjct: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------VS 420

Query: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480
           AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST
Sbjct: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480

Query: 481 SLLFQLF 487
           SLLFQLF
Sbjct: 481 SLLFQLF 481

BLAST of Cp4.1LG01g05880 vs. NCBI nr
Match: XP_022942268.1 (E3 ubiquitin-protein ligase RNFT1-like [Cucurbita moschata])

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 477/487 (97.95%), Postives = 478/487 (98.15%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120
           PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSS SPTSSSSGNTSPSGEVSIRIIGSGEQ
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSTSPTSSSSGNTSPSGEVSIRIIGSGEQ 120

Query: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180
           DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES
Sbjct: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180

Query: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240
           VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK
Sbjct: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240

Query: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILV 300
           QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLL+PLVMLPPKTVPPFWHALFIILV
Sbjct: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHALFIILV 300

Query: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLN 360
           NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFLN
Sbjct: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFLN 360

Query: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVS 420
           KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      VS
Sbjct: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------VS 420

Query: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480
           AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST
Sbjct: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480

Query: 481 SLLFQLF 487
           SLLFQLF
Sbjct: 481 SLLFQLF 481

BLAST of Cp4.1LG01g05880 vs. NCBI nr
Match: KAG6599935.1 (RING finger and transmembrane domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 477/488 (97.75%), Postives = 478/488 (97.95%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTS-SSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGE 120
           PIINGSATPDLRVYSQNQNHLMTTTS SSSSSSS SPTSSSSGNTSPSGEVSIRIIGSGE
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTSPSSSSSSSTSPTSSSSGNTSPSGEVSIRIIGSGE 120

Query: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180
           QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE
Sbjct: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180

Query: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240
           SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK
Sbjct: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240

Query: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIIL 300
           KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLL+PLVMLPPKTVPPFWHALFIIL
Sbjct: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHALFIIL 300

Query: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFL 360
           VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFL
Sbjct: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFL 360

Query: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPV 420
           NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      V
Sbjct: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------V 420

Query: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480
           SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS
Sbjct: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480

Query: 481 TSLLFQLF 487
           TSLLFQLF
Sbjct: 481 TSLLFQLF 482

BLAST of Cp4.1LG01g05880 vs. NCBI nr
Match: XP_022995594.1 (E3 ubiquitin-protein ligase RNFT1-like [Cucurbita maxima])

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 474/488 (97.13%), Postives = 476/488 (97.54%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSS-GNTSPSGEVSIRIIGSGE 120
           PIINGSATPDLRVYSQNQNHLMTTT+SSSSSSS SPTSSSS GNTSPSGEVSIRIIGSGE
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTTSSSSSSSTSPTSSSSSGNTSPSGEVSIRIIGSGE 120

Query: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180
           QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE
Sbjct: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180

Query: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240
           SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK
Sbjct: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240

Query: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIIL 300
           KQTALKGERKISVLAGTSIAFLVHVIGIYWWCH DDLL+PLVMLPPKTVPPFWHALFIIL
Sbjct: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHRDDLLYPLVMLPPKTVPPFWHALFIIL 300

Query: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFL 360
           VNDTM RQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFL
Sbjct: 301 VNDTMVRQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFL 360

Query: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPV 420
           NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      V
Sbjct: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------V 420

Query: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480
           SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS
Sbjct: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480

Query: 481 TSLLFQLF 487
           TSLLFQLF
Sbjct: 481 TSLLFQLF 482

BLAST of Cp4.1LG01g05880 vs. NCBI nr
Match: KAG7030613.1 (RING finger and transmembrane domain-containing protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846 bits (2186), Expect = 5.73e-308
Identity = 451/488 (92.42%), Postives = 452/488 (92.62%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTS-SSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGE 120
           PIINGSATPDLRVYSQNQNHLMTTTS SSSSSSS SPTSSSSGNTSPSGEVSIRIIGSGE
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTSPSSSSSSSTSPTSSSSGNTSPSGEVSIRIIGSGE 120

Query: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180
           QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE
Sbjct: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180

Query: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240
           SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK
Sbjct: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240

Query: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIIL 300
           KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLL+PLVMLPPKTVPPFWHALFIIL
Sbjct: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHALFIIL 300

Query: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFL 360
           VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFL
Sbjct: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFL 360

Query: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPV 420
           NKDQGSLFSSLTTGLYLTFKLTSVVEK                                V
Sbjct: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEK--------------------------------V 420

Query: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480
           SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS
Sbjct: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 456

Query: 481 TSLLFQLF 487
           TSLLFQLF
Sbjct: 481 TSLLFQLF 456

BLAST of Cp4.1LG01g05880 vs. ExPASy TrEMBL
Match: A0A6J1FUD5 (E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC111447364 PE=4 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 477/487 (97.95%), Postives = 478/487 (98.15%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120
           PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSS SPTSSSSGNTSPSGEVSIRIIGSGEQ
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSTSPTSSSSGNTSPSGEVSIRIIGSGEQ 120

Query: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180
           DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES
Sbjct: 121 DPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEES 180

Query: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240
           VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK
Sbjct: 181 VNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVKK 240

Query: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIILV 300
           QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLL+PLVMLPPKTVPPFWHALFIILV
Sbjct: 241 QTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHALFIILV 300

Query: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFLN 360
           NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFLN
Sbjct: 301 NDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFLN 360

Query: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPVS 420
           KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      VS
Sbjct: 361 KDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------VS 420

Query: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480
           AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST
Sbjct: 421 AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGST 480

Query: 481 SLLFQLF 487
           SLLFQLF
Sbjct: 481 SLLFQLF 481

BLAST of Cp4.1LG01g05880 vs. ExPASy TrEMBL
Match: A0A6J1K6D2 (E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita maxima OX=3661 GN=LOC111491080 PE=4 SV=1)

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 474/488 (97.13%), Postives = 476/488 (97.54%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPY NSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSS-GNTSPSGEVSIRIIGSGE 120
           PIINGSATPDLRVYSQNQNHLMTTT+SSSSSSS SPTSSSS GNTSPSGEVSIRIIGSGE
Sbjct: 61  PIINGSATPDLRVYSQNQNHLMTTTTSSSSSSSTSPTSSSSSGNTSPSGEVSIRIIGSGE 120

Query: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180
           QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE
Sbjct: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180

Query: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240
           SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK
Sbjct: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240

Query: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIIL 300
           KQTALKGERKISVLAGTSIAFLVHVIGIYWWCH DDLL+PLVMLPPKTVPPFWHALFIIL
Sbjct: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHRDDLLYPLVMLPPKTVPPFWHALFIIL 300

Query: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFL 360
           VNDTM RQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLP PVWYRFFL
Sbjct: 301 VNDTMVRQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPTPVWYRFFL 360

Query: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPV 420
           NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ      V
Sbjct: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQ------V 420

Query: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480
           SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS
Sbjct: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480

Query: 481 TSLLFQLF 487
           TSLLFQLF
Sbjct: 481 TSLLFQLF 482

BLAST of Cp4.1LG01g05880 vs. ExPASy TrEMBL
Match: A0A5A7VG27 (RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold38G00890 PE=4 SV=1)

HSP 1 Score: 828 bits (2140), Expect = 8.42e-301
Identity = 439/491 (89.41%), Postives = 458/491 (93.28%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METS+SNSDPYSNSSSPTT+ TSSRFG+S+PSLNFFRSPLSVILDYSGILPTRAV  ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120
           P+IN +ATPDLR +SQNQNHL+ T+SSSSS       ++SS NT PSGEVSIRIIGSGEQ
Sbjct: 61  PVINAAATPDLRGFSQNQNHLIPTSSSSSS------VTTSSANTIPSGEVSIRIIGSGEQ 120

Query: 121 DPSPA--LVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVS-ESGNGV 180
           DPSP+  L  Q GYEQNG LPVSGDQPESPDEERVPLVSTASSSIQSG SRV  ESGNG+
Sbjct: 121 DPSPSALLPNQVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGNGM 180

Query: 181 EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDI 240
           EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI ISAVMYKSNDI
Sbjct: 181 EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSNDI 240

Query: 241 VKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFI 300
           VKKQTALKGERKISVLAGTSI FLVHVIGIYWWCHSDDLL+PLVMLPPKTVPPFWHALFI
Sbjct: 241 VKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHALFI 300

Query: 301 ILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRF 360
           ILVNDTM RQ AMALKC LLIYYKNGRGHNFRRQGQILTL+EYTLLLYRALLP PVWYRF
Sbjct: 301 ILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWYRF 360

Query: 361 FLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYI 420
           FLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALS+KEVHYGSYATSEQAS +I
Sbjct: 361 FLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQASKFI 420

Query: 421 -PVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFG 480
             V+AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFG
Sbjct: 421 LSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFG 480

Query: 481 DGSTSLLFQLF 487
           DGSTSLLFQLF
Sbjct: 481 DGSTSLLFQLF 485

BLAST of Cp4.1LG01g05880 vs. ExPASy TrEMBL
Match: A0A6J1FP25 (RING finger and transmembrane domain-containing protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111446950 PE=4 SV=1)

HSP 1 Score: 827 bits (2136), Expect = 3.18e-300
Identity = 436/496 (87.90%), Postives = 460/496 (92.74%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDPYSNSSSPT++ TSSR+GVS+PSLNFFRSPLSVILDYSGILPTRAV HESE
Sbjct: 1   METSASNSDPYSNSSSPTSARTSSRYGVSLPSLNFFRSPLSVILDYSGILPTRAVRHESE 60

Query: 61  PIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGEQ 120
           P+IN ++TPDLRVYSQNQNHLMTT+SSS++S+S       SGNT  SGEVSIRIIGSGEQ
Sbjct: 61  PVINAASTPDLRVYSQNQNHLMTTSSSSTASTS-------SGNTVSSGEVSIRIIGSGEQ 120

Query: 121 DPSPAL--------VGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRV-S 180
           DPSP++        VGQAGYEQN  LP+SGDQPESPDEERVPLVST+SSSIQSGSSRV +
Sbjct: 121 DPSPSVLLPPNQAPVGQAGYEQNARLPISGDQPESPDEERVPLVSTSSSSIQSGSSRVHA 180

Query: 181 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVM 240
           ESGNG EE+ NRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI +SAV+
Sbjct: 181 ESGNGTEETANRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWVSAVL 240

Query: 241 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPF 300
           YKSNDIVKKQTALKGERKI VLAGTSIAFLVHVIGIYWWCHSDDLL PLVMLPPKTVPPF
Sbjct: 241 YKSNDIVKKQTALKGERKIFVLAGTSIAFLVHVIGIYWWCHSDDLLSPLVMLPPKTVPPF 300

Query: 301 WHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPA 360
           WHAL+IILVNDTM RQ+AMA KCLLLIYYKNGRGHNFRRQGQILTL+EY+LLLYRALLPA
Sbjct: 301 WHALYIILVNDTMVRQLAMAFKCLLLIYYKNGRGHNFRRQGQILTLVEYSLLLYRALLPA 360

Query: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSE 420
           PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSE
Sbjct: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSE 420

Query: 421 QASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE 480
           Q      V+AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE
Sbjct: 421 Q------VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE 480

Query: 481 LRTFGDGSTSLLFQLF 487
           LRTFGDGSTSLLFQLF
Sbjct: 481 LRTFGDGSTSLLFQLF 483

BLAST of Cp4.1LG01g05880 vs. ExPASy TrEMBL
Match: A0A6J1CZ45 (RING finger and transmembrane domain-containing protein 1-like OS=Momordica charantia OX=3673 GN=LOC111015921 PE=4 SV=1)

HSP 1 Score: 826 bits (2133), Expect = 1.06e-299
Identity = 441/496 (88.91%), Postives = 463/496 (93.35%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60
           METSASNSDP+SNSSSPTT+ +SSRFG+S+PS+NFFRSPLSVILDYSGILPTRAV HESE
Sbjct: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60

Query: 61  PI-INGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGE 120
           P+ +NG+ATPDLRV+SQN NHL+T +SSSSSSS+    ++SSGN+SPSGEVSIRIIGSGE
Sbjct: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSST---ATTSSGNSSPSGEVSIRIIGSGE 120

Query: 121 QDPSP-------ALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVS- 180
           QDPS        ALVGQAGYEQN G  VSGDQPESPDEERVPLV TASSSIQSGSSRV  
Sbjct: 121 QDPSSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDG 180

Query: 181 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVM 240
           ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI ISAVM
Sbjct: 181 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVM 240

Query: 241 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPF 300
           YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWW H+DDLL+PLVMLPPKTVPPF
Sbjct: 241 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPF 300

Query: 301 WHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPA 360
           WHALFIILVNDTMARQVAMALKC LLIYYKNGRGHNFRRQGQILTL+EYTLLLYRALLP 
Sbjct: 301 WHALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPT 360

Query: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSE 420
           PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALS+KEVHYGSYATSE
Sbjct: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSE 420

Query: 421 QASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE 480
           Q      V+AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE
Sbjct: 421 Q------VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAE 480

Query: 481 LRTFGDGSTSLLFQLF 487
           LRTFGDGSTSLLFQLF
Sbjct: 481 LRTFGDGSTSLLFQLF 487

BLAST of Cp4.1LG01g05880 vs. TAIR 10
Match: AT5G01960.1 (RING/U-box superfamily protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.0e-142
Identity = 277/488 (56.76%), Postives = 334/488 (68.44%), Query Frame = 0

Query: 1   METSASNSDPYSNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVP-HES 60
           MET  ++ D +  S    +  ++S         N  ++P+S +L+YSG+   R  P HE+
Sbjct: 1   METPGASHDSFRGSPRRNSILSAS---------NIIQAPISTLLEYSGLFRARPSPSHEA 60

Query: 61  EPIINGSATPDLRVYSQNQNHLMTTTSSSSSSSSNSPTSSSSGNTSPSGEVSIRIIGSGE 120
           E                      T  S  SS  SN             GEV+IRIIG+ E
Sbjct: 61  E----------------------TLVSDDSSGLSN-------------GEVAIRIIGNTE 120

Query: 121 QDPSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRVSESGNGVEE 180
           QD   A        + G   + G      D                G+S+ +       +
Sbjct: 121 QD---AETDTNALREPGHSELLGSSATQVD---------PMGGASEGASQAAPGDPAAGD 180

Query: 181 SVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVMYKSNDIVK 240
           + +RDS YQRYDIQQ ARWIEQILPFSLLLL+VFIRQHL+GFF+ I I+AVM+KSNDI+K
Sbjct: 181 AASRDSPYQRYDIQQAARWIEQILPFSLLLLVVFIRQHLQGFFVAIWIAAVMFKSNDILK 240

Query: 241 KQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLHPLVMLPPKTVPPFWHALFIIL 300
           KQTALKGER IS L G S+AF  HV+G+YWW   DDLL+PL+MLPPK++PPFWHA+FII+
Sbjct: 241 KQTALKGERHISALIGISVAFTAHVVGVYWWFRKDDLLYPLIMLPPKSIPPFWHAIFIIV 300

Query: 301 VNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPAPVWYRFFL 360
           VNDT+ RQ +M  KC LL+YYKN RG N+R+QGQ+LTL+EY +LLYR+LLP PVWYRFFL
Sbjct: 301 VNDTLVRQASMIFKCFLLMYYKNSRGRNYRKQGQLLTLVEYFMLLYRSLLPTPVWYRFFL 360

Query: 361 NKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSEQASIYIPV 420
           NKD GSLFSSL TGLYLTFKLTSVVEK+QSF  A KALS+KEVHYGSYAT+EQ      V
Sbjct: 361 NKDYGSLFSSLMTGLYLTFKLTSVVEKVQSFFTALKALSRKEVHYGSYATTEQ------V 420

Query: 421 SAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGS 480
           +AAGDLCAICQEKM+ PILLRCKH+FCEDCVSEWFERERTCPLCRALVKPA+L++FGDGS
Sbjct: 421 NAAGDLCAICQEKMHTPILLRCKHMFCEDCVSEWFERERTCPLCRALVKPADLKSFGDGS 426

Query: 481 TSLLFQLF 488
           TSL FQ+F
Sbjct: 481 TSLFFQIF 426

BLAST of Cp4.1LG01g05880 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.9e-08
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 0

Query: 419 VSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 475
           ++A+   C IC+E+M     L C H+F   C+  W ER++TCP CRALV P E  T
Sbjct: 285 LTASDATCIICREEMTNAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENAT 340

BLAST of Cp4.1LG01g05880 vs. TAIR 10
Match: AT1G65040.2 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.8e-08
Identity = 45/136 (33.09%), Postives = 64/136 (47.06%), Query Frame = 0

Query: 340 YTLLLYRALLPAPVWYRFFLNKDQG-SLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALS 399
           + L L R LL   ++  FFL       L   L   LY TF+   +  ++  ++   K  S
Sbjct: 214 FYLELVRDLLHLSMYLCFFLMIFMNYGLPLHLIRELYETFRNFKI--RVTDYLRYRKITS 273

Query: 400 QKEVHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERER 459
                +   AT E+      +S+    C IC+E+M +   L C H+F   C+  W ER+ 
Sbjct: 274 NMNDRFPD-ATPEE------LSSNDATCIICREEMTSAKKLVCGHLFHVHCLRSWLERQN 333

Query: 460 TCPLCRALVKPAELRT 475
           TCP CRALV PAE  T
Sbjct: 334 TCPTCRALVVPAENAT 340

BLAST of Cp4.1LG01g05880 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.8e-08
Identity = 45/136 (33.09%), Postives = 64/136 (47.06%), Query Frame = 0

Query: 340 YTLLLYRALLPAPVWYRFFLNKDQG-SLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALS 399
           + L L R LL   ++  FFL       L   L   LY TF+   +  ++  ++   K  S
Sbjct: 35  FYLELVRDLLHLSMYLCFFLMIFMNYGLPLHLIRELYETFRNFKI--RVTDYLRYRKITS 94

Query: 400 QKEVHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERER 459
                +   AT E+      +S+    C IC+E+M +   L C H+F   C+  W ER+ 
Sbjct: 95  NMNDRFPD-ATPEE------LSSNDATCIICREEMTSAKKLVCGHLFHVHCLRSWLERQN 154

Query: 460 TCPLCRALVKPAELRT 475
           TCP CRALV PAE  T
Sbjct: 155 TCPTCRALVVPAENAT 161

BLAST of Cp4.1LG01g05880 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.8e-08
Identity = 45/136 (33.09%), Postives = 64/136 (47.06%), Query Frame = 0

Query: 340 YTLLLYRALLPAPVWYRFFLNKDQG-SLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALS 399
           + L L R LL   ++  FFL       L   L   LY TF+   +  ++  ++   K  S
Sbjct: 143 FYLELVRDLLHLSMYLCFFLMIFMNYGLPLHLIRELYETFRNFKI--RVTDYLRYRKITS 202

Query: 400 QKEVHYGSYATSEQASIYIPVSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERER 459
                +   AT E+      +S+    C IC+E+M +   L C H+F   C+  W ER+ 
Sbjct: 203 NMNDRFPD-ATPEE------LSSNDATCIICREEMTSAKKLVCGHLFHVHCLRSWLERQN 262

Query: 460 TCPLCRALVKPAELRT 475
           TCP CRALV PAE  T
Sbjct: 263 TCPTCRALVVPAENAT 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q96EX22.5e-3330.23RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=960... [more]
Q5RAG44.8e-3229.90RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=960... [more]
Q9DCN71.5e-3028.62E3 ubiquitin-protein ligase RNFT1 OS=Mus musculus OX=10090 GN=Rnft1 PE=2 SV=1[more]
Q3UF642.6e-3028.83RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=100... [more]
Q6NZ215.8e-3027.36E3 ubiquitin-protein ligase RNFT1 OS=Danio rerio OX=7955 GN=rnft1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_023536798.10.098.77E3 ubiquitin-protein ligase RNFT1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022942268.10.097.95E3 ubiquitin-protein ligase RNFT1-like [Cucurbita moschata][more]
KAG6599935.10.097.75RING finger and transmembrane domain-containing protein 2, partial [Cucurbita ar... [more]
XP_022995594.10.097.13E3 ubiquitin-protein ligase RNFT1-like [Cucurbita maxima][more]
KAG7030613.15.73e-30892.42RING finger and transmembrane domain-containing protein 1, partial [Cucurbita ar... [more]
Match NameE-valueIdentityDescription
A0A6J1FUD50.097.95E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1K6D20.097.13E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita maxima OX=3661 GN=LOC1114910... [more]
A0A5A7VG278.42e-30189.41RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo v... [more]
A0A6J1FP253.18e-30087.90RING finger and transmembrane domain-containing protein 2-like OS=Cucurbita mosc... [more]
A0A6J1CZ451.06e-29988.91RING finger and transmembrane domain-containing protein 1-like OS=Momordica char... [more]
Match NameE-valueIdentityDescription
AT5G01960.12.0e-14256.76RING/U-box superfamily protein [more]
AT3G16090.12.9e-0844.64RING/U-box superfamily protein [more]
AT1G65040.23.8e-0833.09RING/U-box superfamily protein [more]
AT1G65040.13.8e-0833.09RING/U-box superfamily protein [more]
AT1G65040.33.8e-0833.09RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 426..463
e-value: 5.1E-8
score: 42.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 426..464
score: 13.684271
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 424..467
e-value: 2.1E-10
score: 40.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..183
NoneNo IPR availablePANTHERPTHR15860:SF18TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 5..487
NoneNo IPR availableCDDcd16532RING-HC_RNFT1_likecoord: 425..464
e-value: 9.92767E-27
score: 99.6657
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 420..469
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 402..473
e-value: 4.4E-17
score: 63.2
IPR044235RING finger and transmembrane domain-containing protein 1/2PANTHERPTHR15860UNCHARACTERIZED RING FINGER-CONTAINING PROTEINcoord: 5..487
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 441..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g05880.1Cp4.1LG01g05880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1904294 positive regulation of ERAD pathway
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity