Cp4.1LG01g05000 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g05000 vs. ExPASy Swiss-Prot
Match: Q940Z2 (Actin-related protein 5 OS=Arabidopsis thaliana OX=3702 GN=ARP5 PE=1 SV=2)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 626/735 (85.17%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           M FVS I+RQSD+N +  + PIVIDNGAS FRIGWAGE++PRV+FRNIVQRPRHKATGET
Sbjct: 1   MAFVSRIRRQSDYNTYPSSIPIVIDNGASYFRIGWAGETEPRVVFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGD +PS++KYFDCTRS PRS FD NVVYQFEIMEY+LD+ FDRLGANG        
Sbjct: 61  VTIVGDLDPSMMKYFDCTRSGPRSPFDSNVVYQFEIMEYILDYAFDRLGANG-------- 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITEC CNPVQSRSKMAELLFETYGVP++A+GVDAAFSY YNQ  GIC K
Sbjct: 121 ---SGIDHPILITECACNPVQSRSKMAELLFETYGVPAVAFGVDAAFSYKYNQLHGICKK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DG+ LCPGFT +H IPF+DGEP+YKG  RTNIGG+HVTD LKQLLSLKYP+H +RFT EK
Sbjct: 181 DGIVLCPGFTTTHSIPFVDGEPIYKGSSRTNIGGYHVTDYLKQLLSLKYPFHSSRFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
            EDLK+EHCYIA DYASE +LFQ+G KEAE KT  WQLPWIPPPTE PPSEEEIARKAAI
Sbjct: 241 AEDLKLEHCYIAPDYASEIRLFQEGRKEAEEKTSYWQLPWIPPPTEVPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           +EKQGQRLREMAEAKR S+IN++EN++  L FLL Q+ QV E DIP+FLS+TGY S+QE+
Sbjct: 301 REKQGQRLREMAEAKRVSKINDMENQLISLRFLLKQVDQVEEDDIPTFLSDTGYASRQEL 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDA-TNEKFHLVNVPDDVLSPEQLKEKRRQLF 420
           ES +TK TQSLRKA+GEPK++ AE E++ D+  NEK+ L+NVPDD+L+PEQLK+K+RQ+F
Sbjct: 361 ESTITKVTQSLRKARGEPKNEPAEYEENPDSLNNEKYPLMNVPDDILTPEQLKDKKRQMF 420

Query: 421 LKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKR 480
           LKTT+EGR RA+QKR EEELE+E+RNQL+EERR ENPE YLE+L A+YKEV E+VEQ+KR
Sbjct: 421 LKTTAEGRLRARQKRNEEELEKEKRNQLEEERRRENPESYLEELQAQYKEVLERVEQKKR 480

Query: 481 LKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKL 540
           LKTNG  +NGNN SGG+GRGERL+AAQRERMRLLTTAAFDRGKGEDTFG+RDEDWQLYKL
Sbjct: 481 LKTNGS-SNGNNKSGGIGRGERLSAAQRERMRLLTTAAFDRGKGEDTFGSRDEDWQLYKL 540

Query: 541 MSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPK--SDVAQPT-EVPKSRPLTKEDF 600
           MSKD NDDDD+ PD DE EL RLSSRLQ+IDPTFV K   +++Q + EVP+ RPLT+ED+
Sbjct: 541 MSKD-NDDDDEQPDSDEAELARLSSRLQEIDPTFVQKVEGELSQTSGEVPRVRPLTEEDY 600

Query: 601 QIVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGS 660
           +IV+G+ERFRCPEILFHPNL G+DQ GLDEM G S+RRLP    ++ ERLT+SIL+TGG 
Sbjct: 601 KIVIGIERFRCPEILFHPNLIGIDQVGLDEMAGTSIRRLPHDEKELEERLTSSILMTGGC 660

Query: 661 CLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYL 720
            L PGM ERLE GIRMIRPCGSPI VVRA+DPVLDAWRGAS +AA   F    F++MDY 
Sbjct: 661 SLLPGMNERLECGIRMIRPCGSPINVVRAMDPVLDAWRGASAFAANLNFLGNAFTKMDYD 720

Query: 721 EKGEDWLRRYQLRYS 732
           EKGEDWLR YQ+RY+
Sbjct: 721 EKGEDWLRNYQIRYN 722

BLAST of Cp4.1LG01g05000 vs. ExPASy Swiss-Prot
Match: A2WKK5 (Actin-related protein 5 OS=Oryza sativa subsp. indica OX=39946 GN=ARP5 PE=2 SV=2)

HSP 1 Score: 646.0 bits (1665), Expect = 5.2e-184
Identity = 355/591 (60.07%), Postives = 440/591 (74.45%), Query Frame = 0

Query: 146 MAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTASHVIP-FIDGEPVY 205
           M+ELLFETYGVPSI  G D   +Y++   L I    G        A  V   F+ GEPV 
Sbjct: 1   MSELLFETYGVPSI--GND---TYLFVPLLSIV--HGSFEVRIVDAKDVSSYFLKGEPVL 60

Query: 206 KGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYIASDYASEAQLFQK 265
             CCRTN+GGFH+TD L+QLLSLKYPYH A  T EK E+LK EHCY+A DY SE Q+F+ 
Sbjct: 61  GACCRTNVGGFHITDFLRQLLSLKYPYHSASITWEKAEELKKEHCYVALDYMSELQIFKN 120

Query: 266 GTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAIKEKQGQRLREMAEAKRSSRINELE 325
             +EAE KTR WQLPW+PPP EEPPSEEE+ARKAA+KEK GQRLR+MA AKRS +I ELE
Sbjct: 121 NKEEAEEKTRYWQLPWVPPPVEEPPSEEELARKAALKEKAGQRLRDMAAAKRSQKIAELE 180

Query: 326 NEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEIESALTKATQSLRKAKGEPKDDQAE 385
            ++  LE L+ QL    E +  + L  +GY+S+QEI+SA+ KATQSLRKAKGE   +   
Sbjct: 181 KQLSYLEELMEQLDGAEEEEATAILGRSGYLSQQEIKSAILKATQSLRKAKGESNGN--- 240

Query: 386 AEDHTDATN-EKFHLVNVPDDVLSPEQLKEKRRQLFLKTTSEGRQRAKQKRFEEELERER 445
            E+  DA+  +K+ LV+VPD+ L+PEQLKEK++Q+ LKTT+EGR RAKQ+R EEE  RE+
Sbjct: 241 -EEKADASGVDKYPLVSVPDETLTPEQLKEKKKQILLKTTTEGRMRAKQRRAEEEALREK 300

Query: 446 RNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRLKTNGGHANGN-NLSGGVGRGERL 505
           +   +EERRLENPE+YLE+L A+Y E+S++V+QRKR K NGG  NGN N SGGVGRGERL
Sbjct: 301 Q---EEERRLENPELYLEELRARYSELSDRVDQRKRQKLNGGKTNGNHNSSGGVGRGERL 360

Query: 506 NAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLMSKDNNDDDDDGPDEDEVELIRL 565
           NAAQ+ERMRLLT+AAFDRGKGEDTFG RDEDW +YK MSKD NDDDDDG D+DE EL R+
Sbjct: 361 NAAQKERMRLLTSAAFDRGKGEDTFGTRDEDWLVYKKMSKD-NDDDDDGNDDDESELARI 420

Query: 566 SSRLQDIDPTFVPKSDVAQPT-EVPKSRPLTKEDFQIVMGVERFRCPEILFHPNLTGVDQ 625
           +S++QD+DPTFV K++  Q T E PK R LT ED++I +G+ERFRCPEILF P + G+DQ
Sbjct: 421 ASKIQDMDPTFVNKAEAVQQTPEPPKVRTLTAEDYRISIGIERFRCPEILFQPGMIGIDQ 480

Query: 626 TGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIR 685
            G+DEM  +SLRRL    + + ERL  SIL+TGG  L PGM  RLE+GIR  RP  SP++
Sbjct: 481 AGIDEMVSISLRRLME-DEAVKERLCQSILVTGGCSLIPGMIPRLESGIRQFRPYLSPLK 540

Query: 686 VVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGEDWLRRYQLRYSL 733
           +VRA DP++DAWRGA+ +AA ++F   TFS  DY E GE+   RY + YSL
Sbjct: 541 LVRAADPLIDAWRGAAAFAASSKFGRHTFSLADYREHGENLFHRYNIVYSL 575

BLAST of Cp4.1LG01g05000 vs. ExPASy Swiss-Prot
Match: A2ZP58 (Actin-related protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ARP5 PE=3 SV=2)

HSP 1 Score: 646.0 bits (1665), Expect = 5.2e-184
Identity = 355/591 (60.07%), Postives = 440/591 (74.45%), Query Frame = 0

Query: 146 MAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTASHVIP-FIDGEPVY 205
           M+ELLFETYGVPSI  G D   +Y++   L I    G        A  V   F+ GEPV 
Sbjct: 1   MSELLFETYGVPSI--GND---TYLFVPLLSIV--HGSFEVRIVDAKDVSSYFLKGEPVL 60

Query: 206 KGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYIASDYASEAQLFQK 265
             CCRTN+GGFH+TD L+QLLSLKYPYH A  T EK E+LK EHCY+A DY SE Q+F+ 
Sbjct: 61  GACCRTNVGGFHITDFLRQLLSLKYPYHSASITWEKAEELKKEHCYVALDYMSELQIFKN 120

Query: 266 GTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAIKEKQGQRLREMAEAKRSSRINELE 325
             +EAE KTR WQLPW+PPP EEPPSEEE+ARKAA+KEK GQRLR+MA AKRS +I ELE
Sbjct: 121 NKEEAEEKTRYWQLPWVPPPVEEPPSEEELARKAALKEKAGQRLRDMAAAKRSQKIAELE 180

Query: 326 NEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEIESALTKATQSLRKAKGEPKDDQAE 385
            ++  LE L+ QL    E +  + L  +GY+S+QEI+SA+ KATQSLRKAKGE   +   
Sbjct: 181 KQLSYLEELMEQLDGAEEEEATAILGRSGYLSQQEIKSAILKATQSLRKAKGESNGN--- 240

Query: 386 AEDHTDATN-EKFHLVNVPDDVLSPEQLKEKRRQLFLKTTSEGRQRAKQKRFEEELERER 445
            E+  DA+  +K+ LV+VPD+ L+PEQLKEK++Q+ LKTT+EGR RAKQ+R EEE  RE+
Sbjct: 241 -EEKADASGVDKYPLVSVPDETLTPEQLKEKKKQILLKTTTEGRMRAKQRRAEEEALREK 300

Query: 446 RNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRLKTNGGHANGN-NLSGGVGRGERL 505
           +   +EERRLENPE+YLE+L A+Y E+S++V+QRKR K NGG  NGN N SGGVGRGERL
Sbjct: 301 Q---EEERRLENPELYLEELRARYSELSDRVDQRKRQKLNGGKTNGNHNSSGGVGRGERL 360

Query: 506 NAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLMSKDNNDDDDDGPDEDEVELIRL 565
           NAAQ+ERMRLLT+AAFDRGKGEDTFG RDEDW +YK MSKD NDDDDDG D+DE EL R+
Sbjct: 361 NAAQKERMRLLTSAAFDRGKGEDTFGTRDEDWLVYKKMSKD-NDDDDDGNDDDESELARI 420

Query: 566 SSRLQDIDPTFVPKSDVAQPT-EVPKSRPLTKEDFQIVMGVERFRCPEILFHPNLTGVDQ 625
           +S++QD+DPTFV K++  Q T E PK R LT ED++I +G+ERFRCPEILF P + G+DQ
Sbjct: 421 ASKIQDMDPTFVNKAEAVQQTPEPPKVRTLTAEDYRISIGIERFRCPEILFQPGMIGIDQ 480

Query: 626 TGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIR 685
            G+DEM  +SLRRL    + + ERL  SIL+TGG  L PGM  RLE+GIR  RP  SP++
Sbjct: 481 AGIDEMVSISLRRLME-DEAVKERLCQSILVTGGCSLIPGMIPRLESGIRQFRPYLSPLK 540

Query: 686 VVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGEDWLRRYQLRYSL 733
           +VRA DP++DAWRGA+ +AA ++F   TFS  DY E GE+   RY + YSL
Sbjct: 541 LVRAADPLIDAWRGAAAFAASSKFGRHTFSLADYREHGENLFHRYNIVYSL 575

BLAST of Cp4.1LG01g05000 vs. ExPASy Swiss-Prot
Match: Q9Y7X8 (Actin-like protein arp5 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=arp5 PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.4e-88
Identity = 231/743 (31.09%), Postives = 378/743 (50.87%), Query Frame = 0

Query: 13  FNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGETVTIVGDHEPSLL 72
           F + S   P+VIDNG+   R GW GE DP+++F N+V R R +    T T+VG+   +L+
Sbjct: 18  FQNVSNDIPLVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRKLSRTSTLVGN--DTLI 77

Query: 73  KYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLLWQIEQIDHPILI 132
           +    +RS  RS F+ NV+  +++ME VLD+ F +LG             I++++HPI +
Sbjct: 78  EV--GSRSIARSPFERNVISNWDLMEQVLDYTFLKLG-------------IDRMEHPICM 137

Query: 133 TECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTAS 192
           TE + NP   RS M ELLFE Y  PS+AYG+D  FS+ +N +       G+ L  G  AS
Sbjct: 138 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTK---PSSSGIVLNLGNAAS 197

Query: 193 HVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYIA 252
           HVIP ++GE +     R + GG   +  L +L  +KYP    +    + E L  +HC+++
Sbjct: 198 HVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVS 257

Query: 253 SDYASE-AQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAIKEKQGQRLREM 312
           SDY  + A    +   E +      Q P+     +E  S+EE+   A  K + G+RL+  
Sbjct: 258 SDYTHDIAHALDRDILERDEIV--LQFPYTEAAAQE-KSQEELELIAERKRESGRRLQAQ 317

Query: 313 AEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEIESALTKATQSL 372
           A  KR  +  E + E+  L  L  Q + ++       L E G+  + ++ + +      +
Sbjct: 318 AAIKRKEKAAERDRELATLTELQQQSLVLSRRAFQRALEEAGFEDESQLNAQVKNVQAKI 377

Query: 373 RKAKGEPKDDQAEAEDHTDAT----NEKFHLVNVPDDVLSPEQLKEKRRQLFLKTTSEGR 432
           R+A+ + +  Q E+E   D T     + F L+NVPD  L    L++KR Q  +K   + R
Sbjct: 378 RRAQRD-QQRQEESEGSLDVTEIDVEQAFPLLNVPDAELDEAGLRQKRHQRLMKANYDAR 437

Query: 433 QRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRLKTNGGHA 492
            RAK ++  EE     R + DE  RLEN   ++ +    +K + EK+ + KRLK      
Sbjct: 438 VRAKAEKAIEEAAEAERAEADERLRLENFSTWVNEKRETHKILLEKISKNKRLKFE---- 497

Query: 493 NGNNLSGGVGRGERLNAAQRERMRLLTTAAFD--------RGKGEDTFGARDEDWQLYKL 552
                       +R + A + RM+ L T A +        + + ED FGARDEDW++Y  
Sbjct: 498 ----------LNDRKSHASQMRMKSLATLASEQPIQKRKRKDQSEDNFGARDEDWKVYHD 557

Query: 553 MSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPL-------- 612
           +      +++     D++    L  +L + D  F  +++       P++  L        
Sbjct: 558 VLTAEQLEEERKKLLDQI--YSLEKQLLEYDSQFT-QANTYDTLNDPRATLLYAFTRGVS 617

Query: 613 ------TKEDFQIVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAER 672
                   + FQ+ + VE+ R PE++F P++ G+DQ G+ E+    L+R   +S +  ++
Sbjct: 618 DFDVNDVAQAFQLHLNVEQIRVPEVIFSPSIVGIDQAGILEIMRSILQR---HSLEEQQK 677

Query: 673 LTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQF 729
           L +++LITGG    PGM  R++  +  I P GS I V RA +P+LDAW+GAS ++   +F
Sbjct: 678 LVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKF 716

BLAST of Cp4.1LG01g05000 vs. ExPASy Swiss-Prot
Match: Q54E71 (Actin-related protein 5 OS=Dictyostelium discoideum OX=44689 GN=arpE PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.7e-73
Identity = 224/736 (30.43%), Postives = 362/736 (49.18%), Query Frame = 0

Query: 2   PFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGETV 61
           P   +I +     + +P+ PI+IDNG+   R G+A +  P++IFR++V     K    + 
Sbjct: 13  PIDDQICKDYKTKYANPSIPIIIDNGSYQCRAGFANDISPKLIFRSLV----GKVKSTSS 72

Query: 62  TIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLLW 121
            IVG+     LK  D +R + +S FD N++      E + D+ F + G            
Sbjct: 73  PIVGNS----LKEGDISRLTIKSPFDSNLLVHPPSQESIFDYIFHKFGIE---------- 132

Query: 122 QIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICD-- 181
              +I++P+LITE   NP   R  M+ELLFE Y + S+ YG+D+ FS+ Y Q+    D  
Sbjct: 133 --NEIENPVLITEPTSNPTFCRKYMSELLFECYNIKSVVYGIDSLFSF-YGQRDQFKDGG 192

Query: 182 KDGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLE 241
           K+ L +   F  +H+    +    ++   R NIGG   TD L++L+ LKYP H + FT  
Sbjct: 193 KNSLIIGSSFNTTHIYNVQNYNVSHQQTKRINIGGGASTDYLRKLIHLKYPKHKSYFTQN 252

Query: 242 KVEDLKMEHCYIA-SDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKA 301
               +K EHCY++   Y  E + F+   + A+ K+   QLP+     E+   EEE  RK 
Sbjct: 253 YTNKIKEEHCYVSQGQYIEELKEFE-NDQLAKEKSVIIQLPYQEIDFEK--LEEERQRKI 312

Query: 302 AIKEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQ 361
             ++  G +LRE+A+ KR  +  ELE+++  LE +L       E +    L    Y +++
Sbjct: 313 QNRKDLGAKLRELADKKRLEKKTELEDKLASLESILALKTTNVE-EFQQTLKSKSYATEK 372

Query: 362 EIESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQL 421
           +    L +    L+      K +  + ED      E+F L+ + D  L+ +QLKEK++Q 
Sbjct: 373 D----LIRDIDDLKDKLFGKKKESEQVED-----TEEFPLLFIADSELNADQLKEKKKQR 432

Query: 422 FLKTTSEGRQRAKQKRFEE-ELERER---RNQLDEERRLENPEIYLEQLHAKYKEVSEKV 481
            LK+  +GR   K+KR EE E E+E+   R++ +EE  L++PE YL+ LH++  ++ EK 
Sbjct: 433 QLKSMKDGRLAQKRKRDEEKEKEKEKEEERDRQEEESFLKDPEHYLKDLHSRKSKILEKR 492

Query: 482 EQRKRLKTNGGHANGNNLSGGVGRGERLNAAQR-ERMRLLTTAAFDRGKGEDTFGARDED 541
           E R++ K                  ++ N  QR  R+R +         GE         
Sbjct: 493 EARQKQK------------------QKANIVQRNSRLRTIVNPTNHGNYGE--------- 552

Query: 542 WQLYKLMSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTK 601
                       + +D    E+  E+  L   L   DPT +  + V+   + P     T 
Sbjct: 553 ---------KGEEVEDPEEAEESREMAILDKLLNKFDPTSISSAIVSHDDQFPIGEYHTA 612

Query: 602 EDFQIVMGVERFRCPEILFHPN-LTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILI 661
           EDFQ+ +GVER +CPE LF P  + GVDQ GL E    S+  L     D  + +T +I +
Sbjct: 613 EDFQVSLGVERIKCPETLFQPKAIIGVDQMGLVEAIISSI--LSQLPVDTRKLVTENIFL 672

Query: 662 TGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQ--FTTQTF 721
           TGG+      ++R+   I+ IR   SP+ ++++ D  LDAW GA  +    Q  ++  + 
Sbjct: 673 TGGNVNTKHFKDRIHYEIQQIREPYSPLTILKSKDSQLDAWLGARKWCLDNQDNWSNVSI 676

Query: 722 SRMDYLEKGEDWLRRY 727
           S+ DY EKG D+++ +
Sbjct: 733 SKQDYQEKGYDYIKSH 676

BLAST of Cp4.1LG01g05000 vs. NCBI nr
Match: XP_023515177.1 (actin-related protein 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1421 bits (3678), Expect = 0.0
Identity = 721/732 (98.50%), Postives = 721/732 (98.50%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE      
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK
Sbjct: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE
Sbjct: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 721

BLAST of Cp4.1LG01g05000 vs. NCBI nr
Match: KAG7030713.1 (Actin-related protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1419 bits (3674), Expect = 0.0
Identity = 724/748 (96.79%), Postives = 725/748 (96.93%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE    LL
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPESDFGLL 120

Query: 121 ---------------WQI-EQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVD 180
                          W I EQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVD
Sbjct: 121 HSIDFLHNPTILIDSWHISEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVD 180

Query: 181 AAFSYMYNQQLGICDKDGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQL 240
           AAFSYMYNQQLGICDKDGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQL
Sbjct: 181 AAFSYMYNQQLGICDKDGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQL 240

Query: 241 LSLKYPYHMARFTLEKVEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPP 300
           LSLKYPYHMARFTLEKVEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPP
Sbjct: 241 LSLKYPYHMARFTLEKVEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPP 300

Query: 301 TEEPPSEEEIARKAAIKEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVD 360
           TEEPPSEEEIARKAAIKEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVD
Sbjct: 301 TEEPPSEEEIARKAAIKEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVD 360

Query: 361 IPSFLSETGYVSKQEIESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDD 420
           IPSFLSETGYVSKQEIESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDD
Sbjct: 361 IPSFLSETGYVSKQEIESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDD 420

Query: 421 VLSPEQLKEKRRQLFLKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLH 480
           VLSPEQLKEKRRQLFLKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLH
Sbjct: 421 VLSPEQLKEKRRQLFLKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLH 480

Query: 481 AKYKEVSEKVEQRKRLKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGE 540
           AKYKEVSEKVEQRKRLKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGE
Sbjct: 481 AKYKEVSEKVEQRKRLKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGE 540

Query: 541 DTFGARDEDWQLYKLMSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTE 600
           DTFGARDEDWQLYKLMSKDNNDDDD+GPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTE
Sbjct: 541 DTFGARDEDWQLYKLMSKDNNDDDDEGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTE 600

Query: 601 VPKSRPLTKEDFQIVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAE 660
           VPK RPLTKEDFQIVMGVERFRCPEILFHPNL GVDQTGLDEMTGVSLRRLPSYSDDIAE
Sbjct: 601 VPKFRPLTKEDFQIVMGVERFRCPEILFHPNLIGVDQTGLDEMTGVSLRRLPSYSDDIAE 660

Query: 661 RLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQ 720
           RLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQ
Sbjct: 661 RLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQ 720

Query: 721 FTTQTFSRMDYLEKGEDWLRRYQLRYSL 732
           FTTQTFSRMDYLEKGEDWLRRYQLRYSL
Sbjct: 721 FTTQTFSRMDYLEKGEDWLRRYQLRYSL 748

BLAST of Cp4.1LG01g05000 vs. NCBI nr
Match: XP_022943004.1 (actin-related protein 5-like isoform X1 [Cucurbita moschata] >KAG6600043.1 Actin-related protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1415 bits (3662), Expect = 0.0
Identity = 718/732 (98.09%), Postives = 719/732 (98.22%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE      
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK
Sbjct: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPNL GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNLIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE
Sbjct: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 721

BLAST of Cp4.1LG01g05000 vs. NCBI nr
Match: XP_022995168.1 (actin-related protein 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1412 bits (3654), Expect = 0.0
Identity = 716/732 (97.81%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE      
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK
Sbjct: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDEVE+IRLSSRLQDIDPTFVPKSDVAQPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEVEMIRLSSRLQDIDPTFVPKSDVAQPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPNL GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNLIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           G RERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE
Sbjct: 661 GTRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 721

BLAST of Cp4.1LG01g05000 vs. NCBI nr
Match: XP_004147683.1 (actin-related protein 5 isoform X1 [Cucumis sativus] >KGN50081.2 hypothetical protein Csa_021423 [Cucumis sativus])

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 673/732 (91.94%), Postives = 702/732 (95.90%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHF  TTPIVIDNGAS+FRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFPSTTPIVIDNGASAFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDH+PSL+KYFDCTRS PRSAFDGNVVYQFEIMEYVLDFGFDRLGA+GPE      
Sbjct: 61  VTIVGDHDPSLMKYFDCTRSGPRSAFDGNVVYQFEIMEYVLDFGFDRLGASGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHP+LITECVCNPVQSRSKMAELLFETYGVPSIA+GVDAAFSY+YNQQLGICDK
Sbjct: 121 -----IDHPVLITECVCNPVQSRSKMAELLFETYGVPSIAFGVDAAFSYLYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLA+CPGFTASHVIPFIDGEPVYKGCCRTNIGG+HVTDSLKQLLSLKYP+HMARFT EK
Sbjct: 181 DGLAICPGFTASHVIPFIDGEPVYKGCCRTNIGGYHVTDSLKQLLSLKYPHHMARFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAE KTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAEEKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAE DIPSFLSETGY+SKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEDDIPSFLSETGYISKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKD+QAEAEDH DA NEKFHL+NVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDEQAEAEDHADAINEKFHLINVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKR+E ELERERRNQLDEERR+ENPE+YLE+LH KYKE+SEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRYEIELERERRNQLDEERRMENPELYLEELHVKYKELSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGH NG+N+SGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHTNGHNVSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNND+DD+GPDEDE EL+RLSSRLQDIDP+FVPKSDV Q TE+PK RPLTKEDFQIVM
Sbjct: 541 SKDNNDEDDEGPDEDEAELVRLSSRLQDIDPSFVPKSDV-QTTELPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPN  GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNWIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           G+RERLEAGIRMIRPCGSPIRVVRALDP+LDAWRGASVYAA +QFTTQTFSR+DY EKGE
Sbjct: 661 GIRERLEAGIRMIRPCGSPIRVVRALDPILDAWRGASVYAAASQFTTQTFSRLDYYEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 720

BLAST of Cp4.1LG01g05000 vs. ExPASy TrEMBL
Match: A0A6J1FSZ8 (actin-related protein 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447871 PE=3 SV=1)

HSP 1 Score: 1415 bits (3662), Expect = 0.0
Identity = 718/732 (98.09%), Postives = 719/732 (98.22%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE      
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK
Sbjct: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPNL GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNLIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE
Sbjct: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 721

BLAST of Cp4.1LG01g05000 vs. ExPASy TrEMBL
Match: A0A6J1K751 (actin-related protein 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490786 PE=3 SV=1)

HSP 1 Score: 1412 bits (3654), Expect = 0.0
Identity = 716/732 (97.81%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE      
Sbjct: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK
Sbjct: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDEVE+IRLSSRLQDIDPTFVPKSDVAQPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEVEMIRLSSRLQDIDPTFVPKSDVAQPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPNL GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNLIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           G RERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE
Sbjct: 661 GTRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 721

BLAST of Cp4.1LG01g05000 vs. ExPASy TrEMBL
Match: A0A6J1I811 (actin-related protein 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471675 PE=3 SV=1)

HSP 1 Score: 1339 bits (3466), Expect = 0.0
Identity = 674/732 (92.08%), Postives = 698/732 (95.36%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHF  TTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFPSTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDH+PSL+KYFDCTRS  RSAFDGNVVYQFEIMEYVLDFGFDRLGA GPE      
Sbjct: 61  VTIVGDHDPSLMKYFDCTRSGHRSAFDGNVVYQFEIMEYVLDFGFDRLGATGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHP+LITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPVLITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLA+CPGFTASHVIPFIDGEP+YKGCCRTNIGG+HVTDSLKQLLSLKYP+HM +FT EK
Sbjct: 181 DGLAICPGFTASHVIPFIDGEPLYKGCCRTNIGGYHVTDSLKQLLSLKYPHHMTQFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQ+GTKE E KTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQRGTKEVEEKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQV+EVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVSEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTK TQSLRKAKGE KDDQAE E HTDATNEKFHL+NVPDDVLSPEQ+KEKRRQLFL
Sbjct: 361 ESALTKTTQSLRKAKGERKDDQAETEYHTDATNEKFHLINVPDDVLSPEQIKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKR+EEELERERR+QLDEERR+ENPE+YLE+L+ KYKE+SEKVEQRKR+
Sbjct: 421 KTTSEGRQRAKQKRYEEELERERRSQLDEERRMENPELYLEELYVKYKELSEKVEQRKRI 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGH NGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHTNGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDE EL+RLSSRLQDIDPTFV KSDV QPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEAELVRLSSRLQDIDPTFVLKSDV-QPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPN  GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNS+LITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNWIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSVLITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAA +QFTTQTFSRMDY EKGE
Sbjct: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAASQFTTQTFSRMDYYEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 720

BLAST of Cp4.1LG01g05000 vs. ExPASy TrEMBL
Match: A0A6J1GYL6 (actin-related protein 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458360 PE=3 SV=1)

HSP 1 Score: 1336 bits (3458), Expect = 0.0
Identity = 674/732 (92.08%), Postives = 698/732 (95.36%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHF  TTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFPSTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDH+PSL+KYFDCTRS PRSAFDGNVVYQFE MEYVLDFGFDRLGA GPE      
Sbjct: 61  VTIVGDHDPSLMKYFDCTRSGPRSAFDGNVVYQFENMEYVLDFGFDRLGATGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK
Sbjct: 121 -----IDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLA+CPGFTA+HVIPFIDGEP+YKGCCRTNIGG+HVTDSLKQLLSLKYP+HM +FT EK
Sbjct: 181 DGLAICPGFTATHVIPFIDGEPLYKGCCRTNIGGYHVTDSLKQLLSLKYPHHMTQFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYASEAQLFQKGTKE E KTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEVEEKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQV+EVDIPSFLSETGYVSKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVSEVDIPSFLSETGYVSKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTK TQSLRKAKGE KDDQAE EDHTDATNEKFHL+NVPDD+LSPEQ+KEKRRQLFL
Sbjct: 361 ESALTKTTQSLRKAKGERKDDQAETEDHTDATNEKFHLINVPDDMLSPEQIKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKR+EEELERERR+QLDEERR+ENPE+YLE+L+ KYKE+SEKVEQRKR+
Sbjct: 421 KTTSEGRQRAKQKRYEEELERERRSQLDEERRMENPELYLEELYVKYKELSEKVEQRKRI 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGH NGNNLSG VGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHTNGNNLSG-VGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNNDDDD+GPDEDE EL+RLSSRLQDIDPTFV KSDV QPTEVPK RPLTKEDFQIVM
Sbjct: 541 SKDNNDDDDEGPDEDEAELVRLSSRLQDIDPTFVLKSDV-QPTEVPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPN  GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNS+LITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNWIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSVLITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAA +QFTTQTFSRMDY EKGE
Sbjct: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAASQFTTQTFSRMDYYEKGE 719

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 719

BLAST of Cp4.1LG01g05000 vs. ExPASy TrEMBL
Match: A0A5D3BV14 (Actin-related protein 5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00350 PE=3 SV=1)

HSP 1 Score: 1336 bits (3457), Expect = 0.0
Identity = 671/732 (91.67%), Postives = 699/732 (95.49%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           MPFVSEIQRQSDFNHF  TTPIVIDNGAS+FRIGWAGESDPRVIFRNIVQRPRHKATGET
Sbjct: 1   MPFVSEIQRQSDFNHFPSTTPIVIDNGASAFRIGWAGESDPRVIFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGDH+PSL+KYFDCTRS PRSAFDGNVVYQFEIMEYVLDFGFDRLGA+GPE      
Sbjct: 61  VTIVGDHDPSLMKYFDCTRSGPRSAFDGNVVYQFEIMEYVLDFGFDRLGASGPE------ 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHP+LITECVCNPVQSRSKMAELLFETYGVPSIA+GVDAAFSY+YNQQLGICDK
Sbjct: 121 -----IDHPVLITECVCNPVQSRSKMAELLFETYGVPSIAFGVDAAFSYLYNQQLGICDK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DGLA+CPGFTASHVIPFIDGEPVYKGCCRTNIGG+HVTDSLKQLLSLKYP+HMARFT EK
Sbjct: 181 DGLAICPGFTASHVIPFIDGEPVYKGCCRTNIGGYHVTDSLKQLLSLKYPHHMARFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
           VEDLKMEHCYIASDYA EAQLFQKGTKEAE KTRCWQLPWIPPPTEEPPSEEEIARKAAI
Sbjct: 241 VEDLKMEHCYIASDYALEAQLFQKGTKEAEEKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAE DIPSFLSETGY+SKQEI
Sbjct: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEDDIPSFLSETGYISKQEI 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDATNEKFHLVNVPDDVLSPEQLKEKRRQLFL 420
           ESALTKATQSLRKAKGEPKD+QAE EDH DA NEKFHL+NVPDDVLSPEQLKEKRRQLFL
Sbjct: 361 ESALTKATQSLRKAKGEPKDEQAENEDHADAINEKFHLINVPDDVLSPEQLKEKRRQLFL 420

Query: 421 KTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKRL 480
           KTTSEGRQRAKQKR+E ELERERRNQLDEERR+ENPE+YLE+L  KYKE+SEKVEQRKRL
Sbjct: 421 KTTSEGRQRAKQKRYEIELERERRNQLDEERRMENPELYLEELRVKYKELSEKVEQRKRL 480

Query: 481 KTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540
           KTNGGH NG+N+SGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM
Sbjct: 481 KTNGGHTNGHNVSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKLM 540

Query: 541 SKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPKSDVAQPTEVPKSRPLTKEDFQIVM 600
           SKDNND+DD+GPDEDE EL+RLSSRLQDIDPTFVPKSDV Q TE+PK RPLTKEDFQIVM
Sbjct: 541 SKDNNDEDDEGPDEDEAELVRLSSRLQDIDPTFVPKSDV-QTTEMPKFRPLTKEDFQIVM 600

Query: 601 GVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660
           GVERFRCPEILFHPN  GVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP
Sbjct: 601 GVERFRCPEILFHPNWIGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGSCLFP 660

Query: 661 GMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYLEKGE 720
           G+RERLEAGIRMIRPCGSPIRVVRALDP+LDAWRGASVYAA +QFTTQTFSR+DY EKGE
Sbjct: 661 GIRERLEAGIRMIRPCGSPIRVVRALDPILDAWRGASVYAAASQFTTQTFSRLDYYEKGE 720

Query: 721 DWLRRYQLRYSL 732
           DWLRRYQLRYSL
Sbjct: 721 DWLRRYQLRYSL 720

BLAST of Cp4.1LG01g05000 vs. TAIR 10
Match: AT3G12380.1 (actin-related protein 5 )

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 626/735 (85.17%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKATGET 60
           M FVS I+RQSD+N +  + PIVIDNGAS FRIGWAGE++PRV+FRNIVQRPRHKATGET
Sbjct: 1   MAFVSRIRRQSDYNTYPSSIPIVIDNGASYFRIGWAGETEPRVVFRNIVQRPRHKATGET 60

Query: 61  VTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLL 120
           VTIVGD +PS++KYFDCTRS PRS FD NVVYQFEIMEY+LD+ FDRLGANG        
Sbjct: 61  VTIVGDLDPSMMKYFDCTRSGPRSPFDSNVVYQFEIMEYILDYAFDRLGANG-------- 120

Query: 121 WQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDK 180
                IDHPILITEC CNPVQSRSKMAELLFETYGVP++A+GVDAAFSY YNQ  GIC K
Sbjct: 121 ---SGIDHPILITECACNPVQSRSKMAELLFETYGVPAVAFGVDAAFSYKYNQLHGICKK 180

Query: 181 DGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEK 240
           DG+ LCPGFT +H IPF+DGEP+YKG  RTNIGG+HVTD LKQLLSLKYP+H +RFT EK
Sbjct: 181 DGIVLCPGFTTTHSIPFVDGEPIYKGSSRTNIGGYHVTDYLKQLLSLKYPFHSSRFTWEK 240

Query: 241 VEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEIARKAAI 300
            EDLK+EHCYIA DYASE +LFQ+G KEAE KT  WQLPWIPPPTE PPSEEEIARKAAI
Sbjct: 241 AEDLKLEHCYIAPDYASEIRLFQEGRKEAEEKTSYWQLPWIPPPTEVPPSEEEIARKAAI 300

Query: 301 KEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGYVSKQEI 360
           +EKQGQRLREMAEAKR S+IN++EN++  L FLL Q+ QV E DIP+FLS+TGY S+QE+
Sbjct: 301 REKQGQRLREMAEAKRVSKINDMENQLISLRFLLKQVDQVEEDDIPTFLSDTGYASRQEL 360

Query: 361 ESALTKATQSLRKAKGEPKDDQAEAEDHTDA-TNEKFHLVNVPDDVLSPEQLKEKRRQLF 420
           ES +TK TQSLRKA+GEPK++ AE E++ D+  NEK+ L+NVPDD+L+PEQLK+K+RQ+F
Sbjct: 361 ESTITKVTQSLRKARGEPKNEPAEYEENPDSLNNEKYPLMNVPDDILTPEQLKDKKRQMF 420

Query: 421 LKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEKVEQRKR 480
           LKTT+EGR RA+QKR EEELE+E+RNQL+EERR ENPE YLE+L A+YKEV E+VEQ+KR
Sbjct: 421 LKTTAEGRLRARQKRNEEELEKEKRNQLEEERRRENPESYLEELQAQYKEVLERVEQKKR 480

Query: 481 LKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDEDWQLYKL 540
           LKTNG  +NGNN SGG+GRGERL+AAQRERMRLLTTAAFDRGKGEDTFG+RDEDWQLYKL
Sbjct: 481 LKTNGS-SNGNNKSGGIGRGERLSAAQRERMRLLTTAAFDRGKGEDTFGSRDEDWQLYKL 540

Query: 541 MSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPK--SDVAQPT-EVPKSRPLTKEDF 600
           MSKD NDDDD+ PD DE EL RLSSRLQ+IDPTFV K   +++Q + EVP+ RPLT+ED+
Sbjct: 541 MSKD-NDDDDEQPDSDEAELARLSSRLQEIDPTFVQKVEGELSQTSGEVPRVRPLTEEDY 600

Query: 601 QIVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSILITGGS 660
           +IV+G+ERFRCPEILFHPNL G+DQ GLDEM G S+RRLP    ++ ERLT+SIL+TGG 
Sbjct: 601 KIVIGIERFRCPEILFHPNLIGIDQVGLDEMAGTSIRRLPHDEKELEERLTSSILMTGGC 660

Query: 661 CLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTFSRMDYL 720
            L PGM ERLE GIRMIRPCGSPI VVRA+DPVLDAWRGAS +AA   F    F++MDY 
Sbjct: 661 SLLPGMNERLECGIRMIRPCGSPINVVRAMDPVLDAWRGASAFAANLNFLGNAFTKMDYD 720

Query: 721 EKGEDWLRRYQLRYS 732
           EKGEDWLR YQ+RY+
Sbjct: 721 EKGEDWLRNYQIRYN 722

BLAST of Cp4.1LG01g05000 vs. TAIR 10
Match: AT3G12380.2 (actin-related protein 5 )

HSP 1 Score: 1070.8 bits (2768), Expect = 4.7e-313
Identity = 539/741 (72.74%), Postives = 626/741 (84.48%), Query Frame = 0

Query: 1   MPFVSEIQRQSDFNHFSPTTPIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKA---- 60
           M FVS I+RQSD+N +  + PIVIDNGAS FRIGWAGE++PRV+FRNIVQRPRHKA    
Sbjct: 1   MAFVSRIRRQSDYNTYPSSIPIVIDNGASYFRIGWAGETEPRVVFRNIVQRPRHKATVLL 60

Query: 61  --TGETVTIVGDHEPSLLKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPE 120
             TGETVTIVGD +PS++KYFDCTRS PRS FD NVVYQFEIMEY+LD+ FDRLGANG  
Sbjct: 61  SSTGETVTIVGDLDPSMMKYFDCTRSGPRSPFDSNVVYQFEIMEYILDYAFDRLGANG-- 120

Query: 121 DKGRLLWQIEQIDHPILITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQ 180
                      IDHPILITEC CNPVQSRSKMAELLFETYGVP++A+GVDAAFSY YNQ 
Sbjct: 121 ---------SGIDHPILITECACNPVQSRSKMAELLFETYGVPAVAFGVDAAFSYKYNQL 180

Query: 181 LGICDKDGLALCPGFTASHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMA 240
            GIC KDG+ LCPGFT +H IPF+DGEP+YKG  RTNIGG+HVTD LKQLLSLKYP+H +
Sbjct: 181 HGICKKDGIVLCPGFTTTHSIPFVDGEPIYKGSSRTNIGGYHVTDYLKQLLSLKYPFHSS 240

Query: 241 RFTLEKVEDLKMEHCYIASDYASEAQLFQKGTKEAENKTRCWQLPWIPPPTEEPPSEEEI 300
           RFT EK EDLK+EHCYIA DYASE +LFQ+G KEAE KT  WQLPWIPPPTE PPSEEEI
Sbjct: 241 RFTWEKAEDLKLEHCYIAPDYASEIRLFQEGRKEAEEKTSYWQLPWIPPPTEVPPSEEEI 300

Query: 301 ARKAAIKEKQGQRLREMAEAKRSSRINELENEVRGLEFLLHQLVQVAEVDIPSFLSETGY 360
           ARKAAI+EKQGQRLREMAEAKR S+IN++EN++  L FLL Q+ QV E DIP+FLS+TGY
Sbjct: 301 ARKAAIREKQGQRLREMAEAKRVSKINDMENQLISLRFLLKQVDQVEEDDIPTFLSDTGY 360

Query: 361 VSKQEIESALTKATQSLRKAKGEPKDDQAEAEDHTDA-TNEKFHLVNVPDDVLSPEQLKE 420
            S+QE+ES +TK TQSLRKA+GEPK++ AE E++ D+  NEK+ L+NVPDD+L+PEQLK+
Sbjct: 361 ASRQELESTITKVTQSLRKARGEPKNEPAEYEENPDSLNNEKYPLMNVPDDILTPEQLKD 420

Query: 421 KRRQLFLKTTSEGRQRAKQKRFEEELERERRNQLDEERRLENPEIYLEQLHAKYKEVSEK 480
           K+RQ+FLKTT+EGR RA+QKR EEELE+E+RNQL+EERR ENPE YLE+L A+YKEV E+
Sbjct: 421 KKRQMFLKTTAEGRLRARQKRNEEELEKEKRNQLEEERRRENPESYLEELQAQYKEVLER 480

Query: 481 VEQRKRLKTNGGHANGNNLSGGVGRGERLNAAQRERMRLLTTAAFDRGKGEDTFGARDED 540
           VEQ+KRLKTNG  +NGNN SGG+GRGERL+AAQRERMRLLTTAAFDRGKGEDTFG+RDED
Sbjct: 481 VEQKKRLKTNGS-SNGNNKSGGIGRGERLSAAQRERMRLLTTAAFDRGKGEDTFGSRDED 540

Query: 541 WQLYKLMSKDNNDDDDDGPDEDEVELIRLSSRLQDIDPTFVPK--SDVAQPT-EVPKSRP 600
           WQLYKLMSKD NDDDD+ PD DE EL RLSSRLQ+IDPTFV K   +++Q + EVP+ RP
Sbjct: 541 WQLYKLMSKD-NDDDDEQPDSDEAELARLSSRLQEIDPTFVQKVEGELSQTSGEVPRVRP 600

Query: 601 LTKEDFQIVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVSLRRLPSYSDDIAERLTNSI 660
           LT+ED++IV+G+ERFRCPEILFHPNL G+DQ GLDEM G S+RRLP    ++ ERLT+SI
Sbjct: 601 LTEEDYKIVIGIERFRCPEILFHPNLIGIDQVGLDEMAGTSIRRLPHDEKELEERLTSSI 660

Query: 661 LITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLDAWRGASVYAAGAQFTTQTF 720
           L+TGG  L PGM ERLE GIRMIRPCGSPI VVRA+DPVLDAWRGAS +AA   F    F
Sbjct: 661 LMTGGCSLLPGMNERLECGIRMIRPCGSPINVVRAMDPVLDAWRGASAFAANLNFLGNAF 720

Query: 721 SRMDYLEKGEDWLRRYQLRYS 732
           ++MDY EKGEDWLR YQ+RY+
Sbjct: 721 TKMDYDEKGEDWLRNYQIRYN 728

BLAST of Cp4.1LG01g05000 vs. TAIR 10
Match: AT2G37620.1 (actin 1 )

HSP 1 Score: 110.9 bits (276), Expect = 4.3e-24
Identity = 73/260 (28.08%), Postives = 115/260 (44.23%), Query Frame = 0

Query: 21  PIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKAT----GETVTIVGDHEPS-----L 80
           P+V DNG    + G+AG+  PR +F +IV RPRH       G+    VGD   S      
Sbjct: 9   PLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILT 68

Query: 81  LKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLLWQIEQIDHPIL 140
           LKY            +  +V  ++ ME +    F       PE            +HPIL
Sbjct: 69  LKY----------PIEHGIVNNWDDMEKIWHHTFYNELRVAPE------------EHPIL 128

Query: 141 ITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTA 200
           +TE   NP  +R KM +++FET+  P++   + A  S   + +       G+ L  G   
Sbjct: 129 LTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGR-----TTGIVLDSGDGV 188

Query: 201 SHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYI 260
           SH +P  +G  +     R ++ G  +TD+L ++L+ +          E V D+K + CYI
Sbjct: 189 SHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDIKEKLCYI 241

Query: 261 ASDYASEAQLFQKGTKEAEN 272
           A DY  E +  +  +   +N
Sbjct: 249 ALDYEQELETAKTSSSVEKN 241

HSP 2 Score: 82.4 bits (202), Expect = 1.6e-15
Identity = 44/154 (28.57%), Postives = 80/154 (51.95%), Query Frame = 0

Query: 578 DVAQPTEVPKSRPLTKEDFQ------IVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVS 637
           D  Q  E  K+    +++++      I +G ERFRCPE+L+ P++ G++  G+ E T  S
Sbjct: 224 DYEQELETAKTSSSVEKNYELPDGQVITIGSERFRCPEVLYQPSMIGMENAGIHETTYNS 283

Query: 638 LRRLPSYSDDIAERLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLD 697
           + +      DI + L  +I+++GG+ +FPG+ +R+   I  + P    I+VV   +    
Sbjct: 284 IMKC---DVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYS 343

Query: 698 AWRGASVYAAGAQFTTQTFSRMDYLEKGEDWLRR 726
            W G S+ A+ + F     ++ +Y E G   + R
Sbjct: 344 VWIGGSILASLSTFQQMWIAKAEYDESGPSIVHR 374

BLAST of Cp4.1LG01g05000 vs. TAIR 10
Match: AT2G37620.2 (actin 1 )

HSP 1 Score: 110.9 bits (276), Expect = 4.3e-24
Identity = 73/260 (28.08%), Postives = 115/260 (44.23%), Query Frame = 0

Query: 21  PIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKAT----GETVTIVGDHEPS-----L 80
           P+V DNG    + G+AG+  PR +F +IV RPRH       G+    VGD   S      
Sbjct: 9   PLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILT 68

Query: 81  LKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLLWQIEQIDHPIL 140
           LKY            +  +V  ++ ME +    F       PE            +HPIL
Sbjct: 69  LKY----------PIEHGIVNNWDDMEKIWHHTFYNELRVAPE------------EHPIL 128

Query: 141 ITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTA 200
           +TE   NP  +R KM +++FET+  P++   + A  S   + +       G+ L  G   
Sbjct: 129 LTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGR-----TTGIVLDSGDGV 188

Query: 201 SHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYI 260
           SH +P  +G  +     R ++ G  +TD+L ++L+ +          E V D+K + CYI
Sbjct: 189 SHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDIKEKLCYI 241

Query: 261 ASDYASEAQLFQKGTKEAEN 272
           A DY  E +  +  +   +N
Sbjct: 249 ALDYEQELETAKTSSSVEKN 241

HSP 2 Score: 82.4 bits (202), Expect = 1.6e-15
Identity = 44/154 (28.57%), Postives = 80/154 (51.95%), Query Frame = 0

Query: 578 DVAQPTEVPKSRPLTKEDFQ------IVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVS 637
           D  Q  E  K+    +++++      I +G ERFRCPE+L+ P++ G++  G+ E T  S
Sbjct: 224 DYEQELETAKTSSSVEKNYELPDGQVITIGSERFRCPEVLYQPSMIGMENAGIHETTYNS 283

Query: 638 LRRLPSYSDDIAERLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLD 697
           + +      DI + L  +I+++GG+ +FPG+ +R+   I  + P    I+VV   +    
Sbjct: 284 IMKC---DVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYS 343

Query: 698 AWRGASVYAAGAQFTTQTFSRMDYLEKGEDWLRR 726
            W G S+ A+ + F     ++ +Y E G   + R
Sbjct: 344 VWIGGSILASLSTFQQMWIAKAEYDESGPSIVHR 374

BLAST of Cp4.1LG01g05000 vs. TAIR 10
Match: AT3G53750.1 (actin 3 )

HSP 1 Score: 110.9 bits (276), Expect = 4.3e-24
Identity = 73/260 (28.08%), Postives = 115/260 (44.23%), Query Frame = 0

Query: 21  PIVIDNGASSFRIGWAGESDPRVIFRNIVQRPRHKAT----GETVTIVGDHEPS-----L 80
           P+V DNG    + G+AG+  PR +F +IV RPRH       G+    VGD   S      
Sbjct: 9   PLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILT 68

Query: 81  LKYFDCTRSSPRSAFDGNVVYQFEIMEYVLDFGFDRLGANGPEDKGRLLWQIEQIDHPIL 140
           LKY            +  +V  ++ ME +    F       PE            +HPIL
Sbjct: 69  LKY----------PIEHGIVNNWDDMEKIWHHTFYNELRVAPE------------EHPIL 128

Query: 141 ITECVCNPVQSRSKMAELLFETYGVPSIAYGVDAAFSYMYNQQLGICDKDGLALCPGFTA 200
           +TE   NP  +R KM +++FET+  P++   + A  S   + +       G+ L  G   
Sbjct: 129 LTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGR-----TTGIVLDSGDGV 188

Query: 201 SHVIPFIDGEPVYKGCCRTNIGGFHVTDSLKQLLSLKYPYHMARFTLEKVEDLKMEHCYI 260
           SH +P  +G  +     R ++ G  +TD+L ++L+ +          E V D+K + CYI
Sbjct: 189 SHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDIKEKLCYI 241

Query: 261 ASDYASEAQLFQKGTKEAEN 272
           A DY  E +  +  +   +N
Sbjct: 249 ALDYEQELETAKTSSSVEKN 241

HSP 2 Score: 82.4 bits (202), Expect = 1.6e-15
Identity = 44/154 (28.57%), Postives = 80/154 (51.95%), Query Frame = 0

Query: 578 DVAQPTEVPKSRPLTKEDFQ------IVMGVERFRCPEILFHPNLTGVDQTGLDEMTGVS 637
           D  Q  E  K+    +++++      I +G ERFRCPE+L+ P++ G++  G+ E T  S
Sbjct: 224 DYEQELETAKTSSSVEKNYELPDGQVITIGSERFRCPEVLYQPSMIGMENAGIHETTYNS 283

Query: 638 LRRLPSYSDDIAERLTNSILITGGSCLFPGMRERLEAGIRMIRPCGSPIRVVRALDPVLD 697
           + +      DI + L  +I+++GG+ +FPG+ +R+   I  + P    I+VV   +    
Sbjct: 284 IMKC---DVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYS 343

Query: 698 AWRGASVYAAGAQFTTQTFSRMDYLEKGEDWLRR 726
            W G S+ A+ + F     ++ +Y E G   + R
Sbjct: 344 VWIGGSILASLSTFQQMWIAKAEYDESGPSIVHR 374