Cp4.1LG01g04470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g04470 vs. ExPASy Swiss-Prot
Match: Q9Z1A9 (TBC1 domain family member 8 OS=Mus musculus OX=10090 GN=Tbc1d8 PE=1 SV=2)

HSP 1 Score: 157.1 bits (396), Expect = 9.2e-37
Identity = 105/330 (31.82%), Postives = 165/330 (50.00%), Query Frame = 0

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSC 357
           TE++  LV  G+P ++RG LW  F         +  TDL +      N VE         
Sbjct: 494 TEKIRKLVAMGIPESLRGRLWLLF--------SDAVTDLASHPGYYGNLVE--------- 553

Query: 358 SNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVD-GRNALRRLLTAYARHNPSVGYCQ 417
               S    C+  +   +IE+DL R+ P HPA   + G  ALRR+LTAYA  NP +GYCQ
Sbjct: 554 ---QSLGRCCLVTE---EIERDLHRSLPEHPAFQNETGIAALRRVLTAYAHRNPKIGYCQ 613

Query: 418 AMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMV 477
           +MN    +LLL   EE AFW L+ + +     Y++  +I +QVDQ VFEEL++E+ P++ 
Sbjct: 614 SMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKEQLPELA 673

Query: 478 NHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGP 537
            H+  L   +A ++  WFL++F++++P ES + V D   ++G +  +F+  LA++E    
Sbjct: 674 EHMSDLSA-LASISLSWFLTLFLSIMPLESAVHVVDCFFYDGIKA-IFQLGLAVLEANAE 733

Query: 538 ALVTTKDAGDAVTLLQSL-------------AGS-------------TFDSSQLVLTACM 597
            L ++KD G A+ +L                 GS               D + L+  +  
Sbjct: 734 ELCSSKDDGQALMVLSRFLDHIKNEDSPGPPIGSHHAFFSDDQEPYPVTDIADLIRDSYE 793

Query: 598 GFQNVNETRLRELRTKHRPAVVTAIEERSK 601
            F N +  ++  LR KHR  V+   E+ +K
Sbjct: 794 KFGNQSVEQIEHLRCKHRIRVLQGHEDTTK 798

BLAST of Cp4.1LG01g04470 vs. ExPASy Swiss-Prot
Match: O95759 (TBC1 domain family member 8 OS=Homo sapiens OX=9606 GN=TBC1D8 PE=1 SV=3)

HSP 1 Score: 154.5 bits (389), Expect = 6.0e-36
Identity = 105/330 (31.82%), Postives = 164/330 (49.70%), Query Frame = 0

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSC 357
           TE++  LV  G+P ++RG LW  F         +  TDL +      N VE         
Sbjct: 495 TEKIRKLVAMGIPESLRGRLWLLF--------SDAVTDLASHPGYYGNLVE--------- 554

Query: 358 SNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVD-GRNALRRLLTAYARHNPSVGYCQ 417
               S    C+  +   +IE+DL R+ P HPA   + G  ALRR+LTAYA  NP +GYCQ
Sbjct: 555 ---ESLGKCCLVTE---EIERDLHRSLPEHPAFQNETGIAALRRVLTAYAHRNPKIGYCQ 614

Query: 418 AMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMV 477
           +MN    +LLL   EE AFW L+ + +     Y++  +I +QVDQ VFEEL++   P++ 
Sbjct: 615 SMNILTSVLLLYTKEEEAFWLLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKGHLPELA 674

Query: 478 NHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGP 537
            H++ L   +A V+  WFL++F++++P ES + V D   ++G +  +F+  LA++E    
Sbjct: 675 EHMNDLSA-LASVSLSWFLTLFLSIMPLESAVNVVDCFFYDGIKA-IFQLGLAVLEANAE 734

Query: 538 ALVTTKDAGDAVTLLQSL-------------AGS-------------TFDSSQLVLTACM 597
            L ++KD G A+ +L                 GS               D S L+  +  
Sbjct: 735 DLCSSKDDGQALMILSRFLDHIKNEDSPGPPVGSHHAFFSDDQEPYPVTDISDLIRDSYE 794

Query: 598 GFQNVNETRLRELRTKHRPAVVTAIEERSK 601
            F + +  ++  LR KHR  V+   E+ +K
Sbjct: 795 KFGDQSVEQIEHLRYKHRIRVLQGHEDTTK 799

BLAST of Cp4.1LG01g04470 vs. ExPASy Swiss-Prot
Match: Q6ZT07 (TBC1 domain family member 9 OS=Homo sapiens OX=9606 GN=TBC1D9 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 7.8e-36
Identity = 104/332 (31.33%), Postives = 170/332 (51.20%), Query Frame = 0

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVEN--YYTDLLASETISENNVENPSLQSD 357
           TE+   LV  G+P ++RGELW    G    +  +  YY DL+      E ++   +L ++
Sbjct: 505 TEKTRELVLKGIPESMRGELWLLLSGAINEKATHPGYYEDLV------EKSMGKYNLATE 564

Query: 358 SCSNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVD-GRNALRRLLTAYARHNPSVGY 417
                              +IE+DL R+ P HPA   + G  ALRR+LTAYA  NP++GY
Sbjct: 565 -------------------EIERDLHRSLPEHPAFQNEMGIAALRRVLTAYAFRNPNIGY 624

Query: 418 CQAMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPK 477
           CQAMN    +LLL   EE AFW L+ + +     YY+  ++ + VDQ VFEEL R+  P+
Sbjct: 625 CQAMNIVTSVLLLYAKEEEAFWLLVALCERMLPDYYNTRVVGALVDQGVFEELARDYVPQ 684

Query: 478 MVNHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELY 537
           + + +  LGV ++ ++  WFL++F++++P+ES + V D   +EG +V +F+ ALA+++  
Sbjct: 685 LYDCMQDLGV-ISTISLSWFLTLFLSVMPFESAVVVVDCFFYEGIKV-IFQLALAVLDAN 744

Query: 538 GPALVTTKDAGDAVTLLQSLAGST--------------------------FDSSQLVLTA 597
              L+  KD G+A+T+L     S                            D  +L+ T+
Sbjct: 745 VDKLLNCKDDGEAMTVLGRYLDSVTNKDSTLPPIPHLHSLLSDDVEPYPEVDIFRLIRTS 804

Query: 598 CMGFQNVNETRLRELRTKHRPAVVTAIEERSK 601
              F  +    + ++R K R  V+  +E+ +K
Sbjct: 805 YEKFGTIRADLIEQMRFKQRLKVIQTLEDTTK 809

BLAST of Cp4.1LG01g04470 vs. ExPASy Swiss-Prot
Match: Q3UYK3 (TBC1 domain family member 9 OS=Mus musculus OX=10090 GN=Tbc1d9 PE=2 SV=2)

HSP 1 Score: 152.5 bits (384), Expect = 2.3e-35
Identity = 103/331 (31.12%), Postives = 169/331 (51.06%), Query Frame = 0

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVG-VRARRVENYYTDLLASETISENNVENPSLQSDS 357
           TE+   LV  G+P ++RGELW    G +  +     Y + L  +++ + N+         
Sbjct: 505 TEKTRELVLKGIPESMRGELWLLLSGAINEKATHPGYYEGLVEKSMGKYNL--------- 564

Query: 358 CSNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVD-GRNALRRLLTAYARHNPSVGYC 417
                       TE    +IE+DL R+ P HPA   + G  ALRR+LTAYA  NP++GYC
Sbjct: 565 -----------ATE----EIERDLHRSLPEHPAFQNEMGIAALRRVLTAYAFRNPNIGYC 624

Query: 418 QAMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKM 477
           QAMN    +LLL   EE AFW L+ + +     YY+  ++ + VDQ VFEEL R+  P++
Sbjct: 625 QAMNIVTSVLLLYAKEEEAFWLLVALCERMLPDYYNTRVVGALVDQGVFEELARDYVPQL 684

Query: 478 VNHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYG 537
            + +  LGV ++ ++  WFL++F++++P+ES + V D   +EG +V +F+ ALA+++   
Sbjct: 685 YDCMQDLGV-ISTISLSWFLTLFLSVMPFESAVVVVDCFFYEGIKV-IFQLALAVLDANV 744

Query: 538 PALVTTKDAGDAVTLLQSLAGS--------------------------TFDSSQLVLTAC 597
             L+  KD G+A+T+L     S                            D  +L+ T+ 
Sbjct: 745 DKLLNCKDDGEAMTVLGRYLDSVTNKDSTLPPIPHLHSLLSDDVGPYPAVDIFRLIGTSY 804

Query: 598 MGFQNVNETRLRELRTKHRPAVVTAIEERSK 601
             F  +    + ++R K R  V+  +E+ +K
Sbjct: 805 EKFGTIRADLIEQMRFKQRLKVIQTLEDTTK 809

BLAST of Cp4.1LG01g04470 vs. ExPASy Swiss-Prot
Match: Q0IIM8 (TBC1 domain family member 8B OS=Homo sapiens OX=9606 GN=TBC1D8B PE=1 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 5.0e-35
Identity = 101/332 (30.42%), Postives = 166/332 (50.00%), Query Frame = 0

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVEN--YYTDLLASETISENNVENPSLQSD 357
           T++   LV  G+P  +RGELW  F G       N  YYT+++      E ++   +L ++
Sbjct: 477 TKKTRDLVVRGIPETLRGELWMLFSGAVNDMATNPDYYTEVV------EQSLGTCNLATE 536

Query: 358 SCSNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVD-GRNALRRLLTAYARHNPSVGY 417
                              +IE+DL R+ P HPA   D G +ALRR+LTAYA  NP +GY
Sbjct: 537 -------------------EIERDLRRSLPEHPAFQSDTGISALRRVLTAYAYRNPKIGY 596

Query: 418 CQAMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPK 477
           CQAMN    +LLL   EE AFW L+ + +     Y++  +I + VDQ VFEEL+R+  P+
Sbjct: 597 CQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIRDHLPQ 656

Query: 478 MVNHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELY 537
           +  H+  +    + V+  WFL++F+++LP ES + V D   ++G + +L +  LA+++  
Sbjct: 657 LTEHMTDM-TFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAIL-QLGLAILDYN 716

Query: 538 GPALVTTKDAGDAVTLL--------------------------QSLAGSTFDSSQLVLTA 597
              L+T KD  +AVT L                          +  + +  D + L+  +
Sbjct: 717 LDKLLTCKDDAEAVTALNRFFDNVTNKDSPLPSNVQQGSNVSDEKTSHTRVDITDLIRES 776

Query: 598 CMGFQNVNETRLRELRTKHRPAVVTAIEERSK 601
              + N+    +  +R ++R  V+  +EE +K
Sbjct: 777 NEKYGNIRYEDIHSMRCRNRLYVIQTLEETTK 781

BLAST of Cp4.1LG01g04470 vs. NCBI nr
Match: XP_023530055.1 (TBC1 domain family member 8B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1607 bits (4162), Expect = 0.0
Identity = 849/849 (100.00%), Postives = 849/849 (100.00%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD
Sbjct: 541 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK
Sbjct: 781 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 840

Query: 904 LTNVLHDKE 912
           LTNVLHDKE
Sbjct: 841 LTNVLHDKE 849

BLAST of Cp4.1LG01g04470 vs. NCBI nr
Match: XP_022984209.1 (TBC1 domain family member 8B-like [Cucurbita maxima])

HSP 1 Score: 1584 bits (4102), Expect = 0.0
Identity = 837/849 (98.59%), Postives = 841/849 (99.06%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQSLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW EELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWIEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETIS NNVENPSLQSDS SNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISGNNVENPSLQSDSSSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNF AG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFCAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDE+D
Sbjct: 541 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEID 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMV +DNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVEEDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEA 
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           P RK+GLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTT+EEAQNPGVEEK
Sbjct: 781 PTRKIGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTDEEAQNPGVEEK 840

Query: 904 LTNVLHDKE 912
           LTNVLHDKE
Sbjct: 841 LTNVLHDKE 849

BLAST of Cp4.1LG01g04470 vs. NCBI nr
Match: XP_022942547.1 (TBC1 domain family member 8B-like [Cucurbita moschata])

HSP 1 Score: 1583 bits (4098), Expect = 0.0
Identity = 838/849 (98.70%), Postives = 840/849 (98.94%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVNDLSVENDKKAS VEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQSLVNDLSVENDKKASRVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW EELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWIEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDS SNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSSSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLS SESGSTNADEILISLAGEDEVD
Sbjct: 541 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSCSESGSTNADEILISLAGEDEVD 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMV +DNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVKEDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEA 
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           P RK+GLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK
Sbjct: 781 PTRKIGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 840

Query: 904 LTNVLHDKE 912
           LTNV HDKE
Sbjct: 841 LTNVHHDKE 849

BLAST of Cp4.1LG01g04470 vs. NCBI nr
Match: KAG6600091.1 (TBC1 domain family member 8B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1508 bits (3905), Expect = 0.0
Identity = 804/849 (94.70%), Postives = 808/849 (95.17%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVNDLSVENDKKASHVEVVKEEL+SSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQSLVNDLSVENDKKASHVEVVKEELNSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NED+LSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDILSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW EELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWIEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDS SNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSSSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQ                                 ESGSTN DEILISLAGEDEVD
Sbjct: 541 AWKDSQ---------------------------------ESGSTNTDEILISLAGEDEVD 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMV +DNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVKEDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEA 
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           P RK+GLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK
Sbjct: 781 PTRKIGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 816

Query: 904 LTNVLHDKE 912
           LTNV HDKE
Sbjct: 841 LTNVHHDKE 816

BLAST of Cp4.1LG01g04470 vs. NCBI nr
Match: XP_023518916.1 (ecotropic viral integration site 5 ortholog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1436 bits (3718), Expect = 0.0
Identity = 759/856 (88.67%), Postives = 801/856 (93.57%), Query Frame = 0

Query: 58  LVESSPMKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSF 117
           +V+SS MKAAS+AANH++AFDHKRD YGFAVRPQHVQRYREYANIYKEEEEERSERW SF
Sbjct: 1   MVQSSSMKAASKAANHLLAFDHKRDAYGFAVRPQHVQRYREYANIYKEEEEERSERWNSF 60

Query: 118 LDRQSESAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQ 177
           LDRQ+ESAQ LVN+LSVE+D+KASHVE VKEE+DS+IEE T+ ED NS +S  DS+N+  
Sbjct: 61  LDRQAESAQSLVNELSVEDDRKASHVEAVKEEIDSTIEEDTEREDSNSQNSALDSNNVLH 120

Query: 178 NANGLKNEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKL 237
           NANGLKNEDV S K TK+HKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSN+NHDTGTRKL
Sbjct: 121 NANGLKNEDVSSEKDTKTHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNHNHDTGTRKL 180

Query: 238 FSAIEESKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW 297
            SAIEE+KSPRG SEEDSEDEFYDVEKSDPAQEAPSSD+ N  VVGIPAFLLPVESSFPW
Sbjct: 181 LSAIEEAKSPRGASEEDSEDEFYDVEKSDPAQEAPSSDNANGLVVGIPAFLLPVESSFPW 240

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSC 357
            EELEVLVRGGVPMA+RGELWQAFVGVRARRVE YYTDLLAS+T SENN EN SLQSDS 
Sbjct: 241 REELEVLVRGGVPMALRGELWQAFVGVRARRVEKYYTDLLASDTNSENNAENHSLQSDSS 300

Query: 358 SNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 417
           S GSS DSVC TEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA
Sbjct: 301 SKGSSTDSVCTTEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 360

Query: 418 MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVN 477
           MNFFAGLLLLLMPEENAFW LMGIIDDYFDGYYSEEMIESQVDQ VFEELVRERFPKMVN
Sbjct: 361 MNFFAGLLLLLMPEENAFWALMGIIDDYFDGYYSEEMIESQVDQFVFEELVRERFPKMVN 420

Query: 478 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 537
           HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA
Sbjct: 421 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 480

Query: 538 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 597
           LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE
Sbjct: 481 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 540

Query: 598 RSKGLRAWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLA 657
           RSKGLRAWKDSQGLASKLY FKHDSKS++ ET NSSQ+NG LS  ESGSTNA+EILI+L 
Sbjct: 541 RSKGLRAWKDSQGLASKLYSFKHDSKSSMVETMNSSQVNGGLSGPESGSTNAEEILITLP 600

Query: 658 GEDEVDAVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVE 717
           GEDE+D+  +LQDQVVWLKVELCKLLEEKRS+ILRAEELETALMEMV QDNRRQLSARVE
Sbjct: 601 GEDEIDSAPDLQDQVVWLKVELCKLLEEKRSSILRAEELETALMEMVKQDNRRQLSARVE 660

Query: 718 QLEQEVAELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQE 777
           QLEQEVAEL+Q LADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDS AQ+YAAQM QE
Sbjct: 661 QLEQEVAELRQGLADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSAAQKYAAQMFQE 720

Query: 778 KYEEAIVALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQ 837
           KYE+A  ALAEMEKRAVMAESMLEATLQYQSGQL+AQPSPRSVHS PRSLPSDSSLRSSQ
Sbjct: 721 KYEQATTALAEMEKRAVMAESMLEATLQYQSGQLKAQPSPRSVHS-PRSLPSDSSLRSSQ 780

Query: 838 DSAQDFPARKVGLLGRPFGFGWRDKNKGKPSNLEDPN--DGSKTTEEETTIQKKTTEEEA 897
           +SAQDFPARK+GLLGRPFGFGWRDKNKGKPSN+EDPN  DGSKTTEEET+IQKKTTEEE+
Sbjct: 781 ESAQDFPARKIGLLGRPFGFGWRDKNKGKPSNVEDPNPNDGSKTTEEETSIQKKTTEEES 840

Query: 898 QNPGVEEKLTNVLHDK 911
            N GV++K  N LHD+
Sbjct: 841 PNSGVDQKHINGLHDE 855

BLAST of Cp4.1LG01g04470 vs. ExPASy TrEMBL
Match: A0A6J1J9T7 (TBC1 domain family member 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111482595 PE=4 SV=1)

HSP 1 Score: 1584 bits (4102), Expect = 0.0
Identity = 837/849 (98.59%), Postives = 841/849 (99.06%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQSLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW EELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWIEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETIS NNVENPSLQSDS SNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISGNNVENPSLQSDSSSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNF AG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFCAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDE+D
Sbjct: 541 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEID 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMV +DNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVEEDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEA 
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           P RK+GLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTT+EEAQNPGVEEK
Sbjct: 781 PTRKIGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTDEEAQNPGVEEK 840

Query: 904 LTNVLHDKE 912
           LTNVLHDKE
Sbjct: 841 LTNVLHDKE 849

BLAST of Cp4.1LG01g04470 vs. ExPASy TrEMBL
Match: A0A6J1FQK1 (TBC1 domain family member 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447548 PE=4 SV=1)

HSP 1 Score: 1583 bits (4098), Expect = 0.0
Identity = 838/849 (98.70%), Postives = 840/849 (98.94%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE
Sbjct: 1   MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVNDLSVENDKKAS VEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK
Sbjct: 61  SAQSLVNDLSVENDKKASRVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE
Sbjct: 121 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW EELEV
Sbjct: 181 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWIEELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDS SNGSSA
Sbjct: 241 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSSSNGSSA 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG
Sbjct: 301 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLS SESGSTNADEILISLAGEDEVD
Sbjct: 541 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSCSESGSTNADEILISLAGEDEVD 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMV +DNRRQLSARVEQLEQEV
Sbjct: 601 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVKEDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEA 
Sbjct: 661 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
           VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF
Sbjct: 721 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 903
           P RK+GLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK
Sbjct: 781 PTRKIGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEEK 840

Query: 904 LTNVLHDKE 912
           LTNV HDKE
Sbjct: 841 LTNVHHDKE 849

BLAST of Cp4.1LG01g04470 vs. ExPASy TrEMBL
Match: A0A6J1CB22 (TBC1 domain family member 8B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010037 PE=4 SV=1)

HSP 1 Score: 1425 bits (3688), Expect = 0.0
Identity = 758/853 (88.86%), Postives = 795/853 (93.20%), Query Frame = 0

Query: 64  MKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSE 123
           MKAAS+AANH V FDHKRD YGFAVRPQHVQRYREYANIYKEEEEERSE+WKSFLDRQ+E
Sbjct: 1   MKAASKAANHFVTFDHKRDAYGFAVRPQHVQRYREYANIYKEEEEERSEKWKSFLDRQAE 60

Query: 124 SAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLK 183
           SAQ LVN+LSVE++KK SHVEVVKEE+DSSI+E TK EDPN  +S FD SNI QNANGLK
Sbjct: 61  SAQSLVNELSVEDNKKTSHVEVVKEEIDSSIDEDTKREDPNRQNSGFDDSNIPQNANGLK 120

Query: 184 NEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEE 243
           ++DV S K TK HKIQIWTEIRPSLR IE MMSVRVKKKKDLSNNN+DTGTRKL + IEE
Sbjct: 121 SDDVPSEKDTKIHKIQIWTEIRPSLREIEAMMSVRVKKKKDLSNNNNDTGTRKLLTTIEE 180

Query: 244 SKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEV 303
           ++SPRG SEEDSEDEFYDVEKSDPAQEAPS D+VN  VVGIPA LLPVESSFPW EELEV
Sbjct: 181 ARSPRGASEEDSEDEFYDVEKSDPAQEAPSGDNVNGPVVGIPAILLPVESSFPWREELEV 240

Query: 304 LVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSA 363
           LVRGGVPMA+RGELWQ FVGVRARRVE YYTDLLAS+T SENNVEN SLQSDS S GSS 
Sbjct: 241 LVRGGVPMALRGELWQTFVGVRARRVEKYYTDLLASDTNSENNVENHSLQSDSNSKGSST 300

Query: 364 DSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 423
           DSVC TEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG
Sbjct: 301 DSVCTTEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAG 360

Query: 424 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 483
           LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG
Sbjct: 361 LLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLG 420

Query: 484 VQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 543
           VQVAWVTGPWFL+IFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD
Sbjct: 421 VQVAWVTGPWFLTIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKD 480

Query: 544 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLR 603
           AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNE RLRELRTKHRPAVVT IEERSKGLR
Sbjct: 481 AGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNEARLRELRTKHRPAVVTTIEERSKGLR 540

Query: 604 AWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVD 663
           AWK+SQGLASKLY FKHDSKS + ETKN SQINGDLSRSESGSTNAD+ILISL GEDEVD
Sbjct: 541 AWKNSQGLASKLYSFKHDSKSMMIETKNGSQINGDLSRSESGSTNADDILISLTGEDEVD 600

Query: 664 AVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEV 723
           +V +LQ+QV+WLKVELCKLLEEKRSAILRAEELETALMEMV QDNRRQLSARVEQLEQEV
Sbjct: 601 SVPDLQEQVIWLKVELCKLLEEKRSAILRAEELETALMEMVKQDNRRQLSARVEQLEQEV 660

Query: 724 AELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAI 783
           AELQQALADKQEQE AMLQVLMRVEQEQ+LTEDARRFAEQDS AQRYAAQMLQEKYE+A 
Sbjct: 661 AELQQALADKQEQENAMLQVLMRVEQEQRLTEDARRFAEQDSAAQRYAAQMLQEKYEQAT 720

Query: 784 VALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQDSAQDF 843
            ALAEMEKRAVMAESMLEATLQYQSGQL+AQPSPRSVHS PRSLPSDSSLRSSQ+SAQDF
Sbjct: 721 TALAEMEKRAVMAESMLEATLQYQSGQLKAQPSPRSVHS-PRSLPSDSSLRSSQESAQDF 780

Query: 844 PARKVGLLGRPFGFGWRDKNKGKPSNLEDPNDGSKTTEEETTIQKKTTEEEAQNPGVEE- 903
           P RK+GLLGRPFGFGWRDKNKGKPSN+EDPN+GSKT EEE ++QKKTTEEEAQNPGV++ 
Sbjct: 781 PMRKIGLLGRPFGFGWRDKNKGKPSNVEDPNEGSKTVEEEASVQKKTTEEEAQNPGVDQI 840

Query: 904 -KLTNV--LHDKE 912
            K TN   L DKE
Sbjct: 841 QKHTNGNGLLDKE 852

BLAST of Cp4.1LG01g04470 vs. ExPASy TrEMBL
Match: A0A6J1GZ56 (ecotropic viral integration site 5 protein homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458481 PE=4 SV=1)

HSP 1 Score: 1423 bits (3684), Expect = 0.0
Identity = 757/856 (88.43%), Postives = 797/856 (93.11%), Query Frame = 0

Query: 58  LVESSPMKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSF 117
           +V+SS MKAAS+AANH++AFDHKRD YGFAVRPQHVQRYREYANIYKEEEEERSERW SF
Sbjct: 1   MVQSSSMKAASKAANHLLAFDHKRDAYGFAVRPQHVQRYREYANIYKEEEEERSERWNSF 60

Query: 118 LDRQSESAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQ 177
           LDRQ+ESAQ LVN+LSVE+DKKASHVE VKEE DS+IEE T+ ED NS +S  DS+N+  
Sbjct: 61  LDRQAESAQSLVNELSVEDDKKASHVEAVKEERDSTIEEDTEREDSNSQNSALDSNNVLH 120

Query: 178 NANGLKNEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKL 237
           NANGLKNEDV S K TK+HKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSN+NHDTGTRKL
Sbjct: 121 NANGLKNEDVSSEKDTKTHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNHNHDTGTRKL 180

Query: 238 FSAIEESKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW 297
            SAIEE+KSPRG SEEDSEDEFYDVEKSDPAQEAPSSD+ N  VVGIPAFLLPVESSFPW
Sbjct: 181 LSAIEEAKSPRGASEEDSEDEFYDVEKSDPAQEAPSSDNANGLVVGIPAFLLPVESSFPW 240

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSC 357
            EELEVLVRGGVPMA+RGELWQAFVGVRARRVE YYTDLLAS+T SENN EN SLQSDS 
Sbjct: 241 REELEVLVRGGVPMALRGELWQAFVGVRARRVEKYYTDLLASDTNSENNAENHSLQSDSN 300

Query: 358 SNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 417
           S GSS DSVC TEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA
Sbjct: 301 SKGSSTDSVCTTEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 360

Query: 418 MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVN 477
           MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQ VFEELVRERFPKMVN
Sbjct: 361 MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQFVFEELVRERFPKMVN 420

Query: 478 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 537
           HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA
Sbjct: 421 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 480

Query: 538 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 597
           LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE
Sbjct: 481 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 540

Query: 598 RSKGLRAWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLA 657
           RSKGLRA KDSQGLASKLY FK D KS++ ET NSSQ+NG LS  ESGSTNA+EILI+L 
Sbjct: 541 RSKGLRALKDSQGLASKLYSFKLDLKSSMVETMNSSQVNGGLSGPESGSTNAEEILITLP 600

Query: 658 GEDEVDAVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVE 717
           GEDE+D+  +LQDQVVWLKVELCKLLEEKRS+ILRAEELETALMEMV QDNRRQLSARVE
Sbjct: 601 GEDEIDSAPDLQDQVVWLKVELCKLLEEKRSSILRAEELETALMEMVKQDNRRQLSARVE 660

Query: 718 QLEQEVAELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQE 777
           QLEQEVAEL+Q LADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDS AQ+YAAQM QE
Sbjct: 661 QLEQEVAELRQGLADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSAAQKYAAQMFQE 720

Query: 778 KYEEAIVALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQ 837
           KYE+A  ALAEMEKRAVMAESMLEATLQYQSGQL+AQPSPRSVHS PRSLPSDSSLRSSQ
Sbjct: 721 KYEQATTALAEMEKRAVMAESMLEATLQYQSGQLKAQPSPRSVHS-PRSLPSDSSLRSSQ 780

Query: 838 DSAQDFPARKVGLLGRPFGFGWRDKNKGKPSNLEDPN--DGSKTTEEETTIQKKTTEEEA 897
           +SAQDFPARK+GLLGRPFGFGWRDKNKGKPSN+EDPN  DGSKTTEEET+IQKKT EEE+
Sbjct: 781 ESAQDFPARKIGLLGRPFGFGWRDKNKGKPSNVEDPNPNDGSKTTEEETSIQKKTPEEES 840

Query: 898 QNPGVEEKLTNVLHDK 911
            N GV++K  N LHD+
Sbjct: 841 PNSGVDQKHINGLHDE 855

BLAST of Cp4.1LG01g04470 vs. ExPASy TrEMBL
Match: A0A6J1II82 (TBC1 domain family member 2A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477705 PE=4 SV=1)

HSP 1 Score: 1419 bits (3673), Expect = 0.0
Identity = 753/847 (88.90%), Postives = 792/847 (93.51%), Query Frame = 0

Query: 58  LVESSPMKAASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSF 117
           +V+SS MKAAS+AANH++AFDHKRD YGFAVRPQHVQRYREYANIYKEEEEERSERW SF
Sbjct: 1   MVQSSSMKAASKAANHLLAFDHKRDAYGFAVRPQHVQRYREYANIYKEEEEERSERWNSF 60

Query: 118 LDRQSESAQLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQ 177
           LDRQ+ESAQ LVN+LSVE+DKKASHVE VKEE+DS+IEE T+ ED NS +S  DS+N   
Sbjct: 61  LDRQAESAQSLVNELSVEDDKKASHVEAVKEEIDSTIEEDTEREDSNSQNSALDSNNDLH 120

Query: 178 NANGLKNEDVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKL 237
           NANGLKNE V S K TK+HKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSN+NHDTGTRKL
Sbjct: 121 NANGLKNEGVSSEKDTKTHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNHNHDTGTRKL 180

Query: 238 FSAIEESKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPW 297
            SAIEE+KSPRG SEEDSEDEFYDVEKSDPAQEAPSSD+ N  VVGIPAFLLPVESSFPW
Sbjct: 181 LSAIEEAKSPRGASEEDSEDEFYDVEKSDPAQEAPSSDNANGLVVGIPAFLLPVESSFPW 240

Query: 298 TEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSC 357
            EELEVLVRGGVPMA+RGELWQAFVGVRARRVE YYTDLLAS+T SENN EN SLQSDS 
Sbjct: 241 REELEVLVRGGVPMALRGELWQAFVGVRARRVEKYYTDLLASDTNSENNAENHSLQSDSN 300

Query: 358 SNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 417
           S GSS DSVC TEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA
Sbjct: 301 SKGSSTDSVCTTEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQA 360

Query: 418 MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVN 477
           MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQ VFEELVRERFPKMVN
Sbjct: 361 MNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQFVFEELVRERFPKMVN 420

Query: 478 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 537
           HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA
Sbjct: 421 HLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPA 480

Query: 538 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 597
           LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE
Sbjct: 481 LVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEE 540

Query: 598 RSKGLRAWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLA 657
           RSKGLRAWKDSQGLASKLY FKHDSKS++ ET NSSQ+NG L   ESGSTNA+EILI+L 
Sbjct: 541 RSKGLRAWKDSQGLASKLYSFKHDSKSSMVETMNSSQVNGGLLGPESGSTNAEEILITLP 600

Query: 658 GEDEVDAVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVE 717
           GEDE+D+  +LQDQVVWLKVELCKLLEEKRS+ILRAEELETALMEMV QDNRRQLSARVE
Sbjct: 601 GEDEIDSAPDLQDQVVWLKVELCKLLEEKRSSILRAEELETALMEMVKQDNRRQLSARVE 660

Query: 718 QLEQEVAELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQE 777
           QLEQEVAEL+Q LADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDS AQ+YAAQM QE
Sbjct: 661 QLEQEVAELRQGLADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSAAQKYAAQMFQE 720

Query: 778 KYEEAIVALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLPSDSSLRSSQ 837
           KYE+A  ALAEMEKRAVMAESMLEATLQYQSGQL+AQPSPRSVHS PRSLPSDSSLRSSQ
Sbjct: 721 KYEQATSALAEMEKRAVMAESMLEATLQYQSGQLKAQPSPRSVHS-PRSLPSDSSLRSSQ 780

Query: 838 DSAQDFPARKVGLLGRPFGFGWRDKNKGKPSNLEDPN--DGSKTTEEETTIQKKTTEEEA 897
           +SAQDFPARK+GLLGRPFGFGWRDKNKGKPSN+EDPN  DGSKTTEEET+IQKKT EEE+
Sbjct: 781 ESAQDFPARKIGLLGRPFGFGWRDKNKGKPSNVEDPNPNDGSKTTEEETSIQKKTPEEES 840

Query: 898 QNPGVEE 902
            N GV++
Sbjct: 841 PNSGVDQ 846

BLAST of Cp4.1LG01g04470 vs. TAIR 10
Match: AT3G55020.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 969.1 bits (2504), Expect = 2.4e-282
Identity = 531/754 (70.42%), Postives = 615/754 (81.56%), Query Frame = 0

Query: 66  AASQAANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSESA 125
           AAS+ +N +VAF+HKRD YGF VRPQHVQRYREYA+IYKEEEEERS+RW SFL+   ES 
Sbjct: 13  AASKPSNPLVAFEHKRDAYGFPVRPQHVQRYREYADIYKEEEEERSDRWSSFLEDHVEST 72

Query: 126 QLLVNDLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLKNE 185
           +L  N  S       S  E  KE      +E  KG   + H     S     NA+    E
Sbjct: 73  ELPTNGSSENIHAPFSESEKEKE------KELNKGPGEDLHTDKLGSDVTPDNAS---EE 132

Query: 186 DVLSAKGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEESK 245
           +         H++Q+WTEIRPSLR+IED+MS+RVKKK DLS +  +    K+  + +++K
Sbjct: 133 EGHPDAEKNVHRVQLWTEIRPSLRSIEDLMSIRVKKKGDLSKSEQEAPKVKISPSFDDAK 192

Query: 246 SPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEVLV 305
           S +G S+ DSEDEFYDVE+SD  Q+  SSD      + + A   P+ S+ PW EELEVL+
Sbjct: 193 SSKGASDIDSEDEFYDVERSD-VQDGSSSDGTGVSGIPVAADASPL-STCPWKEELEVLI 252

Query: 306 RGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSADS 365
           RGGVPMA+RGELWQAFVGVR RR ++YY +LLA++  S N +E   +Q      GSS +S
Sbjct: 253 RGGVPMALRGELWQAFVGVRKRRCKDYYQNLLAADG-SVNTIEQEDMQHVD-DKGSSTES 312

Query: 366 VCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAGLL 425
           + + EKWKGQIEKDLPRTFPGHPALD DGRNALRRLLTAYARHNPSVGYCQAMNFFA LL
Sbjct: 313 IAVVEKWKGQIEKDLPRTFPGHPALDDDGRNALRRLLTAYARHNPSVGYCQAMNFFAALL 372

Query: 426 LLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLGVQ 485
           LLLMPEENAFW L+G+IDDYF+GYYSEEMIESQVDQLV EELVRERFPK+V+HLDYLGVQ
Sbjct: 373 LLLMPEENAFWALIGLIDDYFNGYYSEEMIESQVDQLVLEELVRERFPKLVHHLDYLGVQ 432

Query: 486 VAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKDAG 545
           VAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEG RVMLFRTALALMELYGPALVTTKDAG
Sbjct: 433 VAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGTRVMLFRTALALMELYGPALVTTKDAG 492

Query: 546 DAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLRAW 605
           DAVTLLQSL GSTFDSSQLVLTACMG+QNV+E RL+ELR+KHRPAV+ A+EERSKGL+AW
Sbjct: 493 DAVTLLQSLTGSTFDSSQLVLTACMGYQNVHEIRLQELRSKHRPAVIAALEERSKGLQAW 552

Query: 606 KDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVDAV 665
           +DS+GLASKLY FK D KS + ++K +S  NG LSRSESGS+NADE+L+SL G+ EVD+V
Sbjct: 553 RDSKGLASKLYNFKQDPKSVLVDSK-ASLSNGSLSRSESGSSNADEVLVSLTGDGEVDSV 612

Query: 666 SNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEVAE 725
            +LQ QV+WLK ELCKLLEEKRSA+LRAEELE ALME+V +DNRRQLSA+VEQLEQE+AE
Sbjct: 613 QDLQAQVLWLKAELCKLLEEKRSALLRAEELEIALMEIVKEDNRRQLSAKVEQLEQEMAE 672

Query: 726 LQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAIVA 785
           +Q+ L+DKQEQE AMLQVLMRVEQEQK+TEDAR FAEQD+ AQRYAAQ+LQEKYEEA+ A
Sbjct: 673 VQRLLSDKQEQEGAMLQVLMRVEQEQKVTEDARIFAEQDAEAQRYAAQVLQEKYEEAVAA 732

Query: 786 LAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRS 820
           LAEMEKRAVMAESMLEATLQYQSGQL+AQPSPR+
Sbjct: 733 LAEMEKRAVMAESMLEATLQYQSGQLKAQPSPRT 752

BLAST of Cp4.1LG01g04470 vs. TAIR 10
Match: AT2G39280.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 881.3 bits (2276), Expect = 6.6e-256
Identity = 482/750 (64.27%), Postives = 579/750 (77.20%), Query Frame = 0

Query: 71  ANHVVAFDHKRDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSESAQLLVN 130
           A+ V   DHKRD YGF+VRPQHVQRYREY NIYKEEE ERS RW +FL+  +ES     N
Sbjct: 3   ADAVSKSDHKRDAYGFSVRPQHVQRYREYVNIYKEEEVERSARWSNFLEFHAESGVSPTN 62

Query: 131 DLSVENDKKASHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLKNEDVLSA 190
             S       S  +  KE      +E  KG +    ++D   S++       + ED +  
Sbjct: 63  GSSENTHVNPSESDKKKE------KELNKGAERKDLNADKPGSDL--TPGNAREEDEVPN 122

Query: 191 KGTKSHKIQIWTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEESKSPRGV 250
           +    HK+Q+W EIRPSL+AIED+MSVRVK K D +N   +       ++ +E++S +GV
Sbjct: 123 REKNVHKVQLWAEIRPSLQAIEDLMSVRVKMKGDSTNGEQEAQKLNSLASTDETESSKGV 182

Query: 251 SEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEVLVRGGVP 310
            E DSEDEFYD E+SDP Q+  SSD  +   +   A    + S+ PW +ELEVL+ GG P
Sbjct: 183 CENDSEDEFYDAERSDPIQDG-SSDGTSVSSMSAAADAASLVSACPWKDELEVLIHGGAP 242

Query: 311 MAIRGELWQAFVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSADSVCITE 370
           MA+RGELWQAF GV+ RRV+NYY +LLA++++  N++E   +Q      GSS D + + E
Sbjct: 243 MALRGELWQAFAGVKKRRVKNYYQNLLAADSLG-NDIEQELMQHTD-EKGSSTDPLSVVE 302

Query: 371 KWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAGLLLLLMP 430
           KWKGQIEKDLPRTFPGHPALD D RNALRRLLTAYARHNPSVGYCQAMNFFA LLLLLMP
Sbjct: 303 KWKGQIEKDLPRTFPGHPALDDDFRNALRRLLTAYARHNPSVGYCQAMNFFAALLLLLMP 362

Query: 431 EENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLGVQVAWVT 490
           EENAFW+L GIIDDYF  YYSEEM+ESQVDQ V EEL+RERFPK+V+HLDYLGVQVA VT
Sbjct: 363 EENAFWSLTGIIDDYFHDYYSEEMLESQVDQRVLEELLRERFPKLVHHLDYLGVQVACVT 422

Query: 491 GPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKDAGDAVTL 550
           GPWFL+IF+NMLPWESVLRVWDVLLFEGNRVMLFRTALALME YGPALVTTKD GDAVTL
Sbjct: 423 GPWFLTIFINMLPWESVLRVWDVLLFEGNRVMLFRTALALMEFYGPALVTTKDIGDAVTL 482

Query: 551 LQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLRAWKDSQG 610
           LQS+ GSTFDSSQLV TACMG+QNV+E++L+ELR+KHRPAV+ A EER KGL+AW+DS+ 
Sbjct: 483 LQSMTGSTFDSSQLVFTACMGYQNVHESKLQELRSKHRPAVIAAFEERLKGLQAWRDSKD 542

Query: 611 LASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVDAVSNLQD 670
            A+KL+  K D  S +     +S  NG LSRSESGS+ AD+I ISL G+ E+D   +LQ 
Sbjct: 543 TATKLHNSKQDPNSVL--ASKASLSNGSLSRSESGSSYADDIFISLTGDCEIDIFQDLQG 602

Query: 671 QVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEVAELQQAL 730
           QV+WLK EL KLL+EKRSA+LRAEELE ALMEMV QDNRRQL A++EQLEQ V EL++ +
Sbjct: 603 QVLWLKGELHKLLQEKRSALLRAEELEVALMEMVKQDNRRQLKAKIEQLEQGVTELRRLV 662

Query: 731 ADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAIVALAEME 790
           +DK+EQE+AM+QVLMR+EQE K+TEDARR AEQD+  QRYAA++LQEKYEEA+ ALAEME
Sbjct: 663 SDKREQESAMIQVLMRMEQEHKVTEDARRLAEQDAATQRYAAEVLQEKYEEAVAALAEME 722

Query: 791 KRAVMAESMLEATLQYQSGQLRAQPSPRSV 821
           +RAVMAESMLEATLQYQSGQ++AQPSPR +
Sbjct: 723 ERAVMAESMLEATLQYQSGQVKAQPSPRQL 739

BLAST of Cp4.1LG01g04470 vs. TAIR 10
Match: AT2G39280.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 857.8 bits (2215), Expect = 7.8e-249
Identity = 473/740 (63.92%), Postives = 568/740 (76.76%), Query Frame = 0

Query: 81  RDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQSESAQLLVNDLSVENDKKA 140
           RD YGF+VRPQHVQRYREY NIYKEEE ERS RW +FL+  +ES     N  S       
Sbjct: 41  RDAYGFSVRPQHVQRYREYVNIYKEEEVERSARWSNFLEFHAESGVSPTNGSSENTHVNP 100

Query: 141 SHVEVVKEELDSSIEEGTKGEDPNSHDSDFDSSNIAQNANGLKNEDVLSAKGTKSHKIQI 200
           S  +  KE      +E  KG +    ++D   S++       + ED +  +    HK+Q+
Sbjct: 101 SESDKKKE------KELNKGAERKDLNADKPGSDL--TPGNAREEDEVPNREKNVHKVQL 160

Query: 201 WTEIRPSLRAIEDMMSVRVKKKKDLSNNNHDTGTRKLFSAIEESKSPRGVSEEDSEDEFY 260
           W EIRPSL+AIED+MSVRVK K D +N   +       ++ +E++S +GV E DSEDEFY
Sbjct: 161 WAEIRPSLQAIEDLMSVRVKMKGDSTNGEQEAQKLNSLASTDETESSKGVCENDSEDEFY 220

Query: 261 DVEKSDPAQEAPSSDSVNSHVVGIPAFLLPVESSFPWTEELEVLVRGGVPMAIRGELWQA 320
           D E+SDP Q+  SSD  +   +   A    + S+ PW +ELEVL+ GG PMA+RGELWQA
Sbjct: 221 DAERSDPIQDG-SSDGTSVSSMSAAADAASLVSACPWKDELEVLIHGGAPMALRGELWQA 280

Query: 321 FVGVRARRVENYYTDLLASETISENNVENPSLQSDSCSNGSSADSVCITEKWKGQIEKDL 380
           F GV+ RRV+NYY +LLA++++  N++E   +Q      GSS D + + EKWKGQIEKDL
Sbjct: 281 FAGVKKRRVKNYYQNLLAADSLG-NDIEQELMQHTD-EKGSSTDPLSVVEKWKGQIEKDL 340

Query: 381 PRTFPGHPALDVDGRNALRRLLTAYARHNPSVGYCQAMNFFAGLLLLLMPEENAFWTLMG 440
           PRTFPGHPALD D RNALRRLLTAYARHNPSVGYCQAMNFFA LLLLLMPEENAFW+L G
Sbjct: 341 PRTFPGHPALDDDFRNALRRLLTAYARHNPSVGYCQAMNFFAALLLLLMPEENAFWSLTG 400

Query: 441 IIDDYFDGYYSEEMIESQVDQLVFEELVRERFPKMVNHLDYLGVQVAWVTGPWFLSIFMN 500
           IIDDYF  YYSEEM+ESQVDQ V EEL+RERFPK+V+HLDYLGVQVA VTGPWFL+IF+N
Sbjct: 401 IIDDYFHDYYSEEMLESQVDQRVLEELLRERFPKLVHHLDYLGVQVACVTGPWFLTIFIN 460

Query: 501 MLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKDAGDAVTLLQSLAGSTFD 560
           MLPWESVLRVWDVLLFEGNRVMLFRTALALME YGPALVTTKD GDAVTLLQS+ GSTFD
Sbjct: 461 MLPWESVLRVWDVLLFEGNRVMLFRTALALMEFYGPALVTTKDIGDAVTLLQSMTGSTFD 520

Query: 561 SSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLRAWKDSQGLASKLYIFKH 620
           SSQLV TACMG+QNV+E++L+ELR+KHRPAV+ A EER KGL+AW+DS+  A+KL+  K 
Sbjct: 521 SSQLVFTACMGYQNVHESKLQELRSKHRPAVIAAFEERLKGLQAWRDSKDTATKLHNSKQ 580

Query: 621 DSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVDAVSNLQDQVVWLKVELC 680
           D  S +     +S  NG LSRSESGS+ AD+I ISL G+ E+D   +LQ Q      EL 
Sbjct: 581 DPNSVL--ASKASLSNGSLSRSESGSSYADDIFISLTGDCEIDIFQDLQGQ-----GELH 640

Query: 681 KLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEVAELQQALADKQEQETAM 740
           KLL+EKRSA+LRAEELE ALMEMV QDNRRQL A++EQLEQ V EL++ ++DK+EQE+AM
Sbjct: 641 KLLQEKRSALLRAEELEVALMEMVKQDNRRQLKAKIEQLEQGVTELRRLVSDKREQESAM 700

Query: 741 LQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAIVALAEMEKRAVMAESML 800
           +QVLMR+EQE K+TEDARR AEQD+  QRYAA++LQEKYEEA+ ALAEME+RAVMAESML
Sbjct: 701 IQVLMRMEQEHKVTEDARRLAEQDAATQRYAAEVLQEKYEEAVAALAEMEERAVMAESML 760

Query: 801 EATLQYQSGQLRAQPSPRSV 821
           EATLQYQSGQ++AQPSPR +
Sbjct: 761 EATLQYQSGQVKAQPSPRQL 762

BLAST of Cp4.1LG01g04470 vs. TAIR 10
Match: AT2G37290.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 734.2 bits (1894), Expect = 1.3e-211
Identity = 463/909 (50.94%), Postives = 574/909 (63.15%), Query Frame = 0

Query: 81  RDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQ------------------- 140
           RD YGFA+RPQHVQRY+EY +IY EEE ER+E+WK+FLDRQ                   
Sbjct: 16  RDAYGFALRPQHVQRYQEYLSIYTEEETERAEKWKNFLDRQEDRTAEPCSSDEEFQDTFQ 75

Query: 141 -----------SESAQLLVN------------DLSVENDKKASHVEVVKEE--------L 200
                      SES +   N            +  +E D+  +  +V KE         L
Sbjct: 76  ADGLESGEDSDSESEEGSRNGKHEDCELSGTSEQQLEQDRTETVGDVSKETEPAEEALVL 135

Query: 201 DSSIE---EGTKGEDPNSHDSDFDSS-------------------------NIAQNANGL 260
           D S+    E  K E+    +SD D                           +I Q    +
Sbjct: 136 DKSLRSDTESVKDEEEEKFESDKDKESSVGSESESDEEQQSQAVKEPVDHVHIQQEEKLV 195

Query: 261 KNEDVLSA-------KGTKSHKIQI-WTEIRPSLRAIEDMMSVRVKKKKDLSN------N 320
             ED   +       K TK+ +  I W  IRP L +IEDMM  RVK  K   N      +
Sbjct: 196 AEEDKCESGHEEKAQKETKAPRSVIEWAHIRPCLASIEDMMCSRVKNVKSTKNGQKNIVD 255

Query: 321 NHDTGTRKLFSAIEESKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFL 380
           +H +  ++  S+IEES    G ++ DSE             EA  S S            
Sbjct: 256 DHASSIKESLSSIEES----GENDRDSETSTSRSHSIKEENEAQGSVS------------ 315

Query: 381 LPVESSFPWTEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVE 440
              E  FPW EELEVLVR GVP  +RGE+WQAFVGV+ARRVE YY DLLA  T S+ N  
Sbjct: 316 --PEPFFPWYEELEVLVRLGVPKDLRGEVWQAFVGVKARRVERYYQDLLAQITNSDEN-- 375

Query: 441 NPSLQSDSCSNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARH 500
                        S+D   +  KWK QIEKD+PRTFPGHPAL+ +GR++LRR+L AYA H
Sbjct: 376 -------------SSD---VQRKWKKQIEKDIPRTFPGHPALNENGRDSLRRILLAYACH 435

Query: 501 NPSVGYCQAMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELV 560
           NPSVGYCQAMNFFAGLLLLLMPEENAFWTL+GIIDDYFDGYY+EEMIESQVDQLVFEEL+
Sbjct: 436 NPSVGYCQAMNFFAGLLLLLMPEENAFWTLVGIIDDYFDGYYTEEMIESQVDQLVFEELM 495

Query: 561 RERFPKMVNHLDYLGVQVAWVTGPWFLSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTAL 620
           RERFPK+VNHLDYLGVQVAW++GPWFLSIF+N++PWE VLR+WDVLLFEGNRV+LFRTA 
Sbjct: 496 RERFPKLVNHLDYLGVQVAWISGPWFLSIFVNIIPWECVLRMWDVLLFEGNRVVLFRTAF 555

Query: 621 ALMELYGPALVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGFQNVNETRLRELRTKHR 680
           A+MELYGPA+V TKDAGDA+T LQSLA STFDSSQLVLTACMG+ + NE RL ELR  HR
Sbjct: 556 AIMELYGPAIVATKDAGDAITSLQSLASSTFDSSQLVLTACMGYISTNEARLEELRKIHR 615

Query: 681 PAVVTAIEERSKGLRAWKDSQGLASKLYIFKHDSKSTITETKNSSQINGDLSRSESGSTN 740
           PAV+  +EER +  R WKD +GLASKLY FKH+      E K++ + +G+    +  S +
Sbjct: 616 PAVLEIVEERIQKGRVWKDKKGLASKLYSFKHEGSILDHEQKSTQRNDGENQDDDDESCS 675

Query: 741 ADEILISLAGEDEVDAVSNLQDQVVWLKVELCKLLEEKRSAILRAEELETALMEMVTQDN 800
               L     + EVD++ +LQ+QVVW+KVELC+LLEEKRSA++RAEELE ALMEMV +DN
Sbjct: 676 PFLNLDGANVDSEVDSLPDLQEQVVWMKVELCRLLEEKRSAVMRAEELEIALMEMVKEDN 735

Query: 801 RRQLSARVEQLEQEVAELQQALADKQEQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQ 860
           R +LSAR+EQLE++V EL+Q L+DK+EQETAMLQVLM+VEQ+QKLTEDAR  AEQD+ AQ
Sbjct: 736 RLELSARIEQLERDVRELKQVLSDKKEQETAMLQVLMKVEQDQKLTEDARINAEQDAAAQ 795

Query: 861 RYAAQMLQEKYEEAIVALAEMEKRAVMAESMLEATLQYQSGQLRAQPSPRSVHSSPRSLP 898
           RYA  +LQEK E+ +  LA+MEK+ V AE+ LEATLQY+SGQ +A      + SSPR   
Sbjct: 796 RYAVHVLQEKNEKLVTQLAQMEKKLVTAETTLEATLQYESGQNKA------LSSSPR--- 855

BLAST of Cp4.1LG01g04470 vs. TAIR 10
Match: AT2G37290.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 716.8 bits (1849), Expect = 2.1e-206
Identity = 463/943 (49.10%), Postives = 574/943 (60.87%), Query Frame = 0

Query: 81  RDTYGFAVRPQHVQRYREYANIYKEEEEERSERWKSFLDRQ------------------- 140
           RD YGFA+RPQHVQRY+EY +IY EEE ER+E+WK+FLDRQ                   
Sbjct: 16  RDAYGFALRPQHVQRYQEYLSIYTEEETERAEKWKNFLDRQEDRTAEPCSSDEEFQDTFQ 75

Query: 141 -----------SESAQLLVN------------DLSVENDKKASHVEVVKEE--------L 200
                      SES +   N            +  +E D+  +  +V KE         L
Sbjct: 76  ADGLESGEDSDSESEEGSRNGKHEDCELSGTSEQQLEQDRTETVGDVSKETEPAEEALVL 135

Query: 201 DSSIE---EGTKGEDPNSHDSDFDSS-------------------------NIAQNANGL 260
           D S+    E  K E+    +SD D                           +I Q    +
Sbjct: 136 DKSLRSDTESVKDEEEEKFESDKDKESSVGSESESDEEQQSQAVKEPVDHVHIQQEEKLV 195

Query: 261 KNEDVLSA-------KGTKSHKIQI-WTEIRPSLRAIEDMMSVRVKKKKDLSN------N 320
             ED   +       K TK+ +  I W  IRP L +IEDMM  RVK  K   N      +
Sbjct: 196 AEEDKCESGHEEKAQKETKAPRSVIEWAHIRPCLASIEDMMCSRVKNVKSTKNGQKNIVD 255

Query: 321 NHDTGTRKLFSAIEESKSPRGVSEEDSEDEFYDVEKSDPAQEAPSSDSVNSHVVGIPAFL 380
           +H +  ++  S+IEES    G ++ DSE             EA  S S            
Sbjct: 256 DHASSIKESLSSIEES----GENDRDSETSTSRSHSIKEENEAQGSVS------------ 315

Query: 381 LPVESSFPWTEELEVLVRGGVPMAIRGELWQAFVGVRARRVENYYTDLLASETISENNVE 440
              E  FPW EELEVLVR GVP  +RGE+WQAFVGV+ARRVE YY DLLA  T S+ N  
Sbjct: 316 --PEPFFPWYEELEVLVRLGVPKDLRGEVWQAFVGVKARRVERYYQDLLAQITNSDEN-- 375

Query: 441 NPSLQSDSCSNGSSADSVCITEKWKGQIEKDLPRTFPGHPALDVDGRNALRRLLTAYARH 500
                        S+D   +  KWK QIEKD+PRTFPGHPAL+ +GR++LRR+L AYA H
Sbjct: 376 -------------SSD---VQRKWKKQIEKDIPRTFPGHPALNENGRDSLRRILLAYACH 435

Query: 501 NPSVGYCQAMNFFAGLLLLLMPEENAFWTLMGIIDDYFDGYYSEEMIESQVDQLVFEELV 560
           NPSVGYCQAMNFFAGLLLLLMPEENAFWTL+GIIDDYFDGYY+EEMIESQVDQLVFEEL+
Sbjct: 436 NPSVGYCQAMNFFAGLLLLLMPEENAFWTLVGIIDDYFDGYYTEEMIESQVDQLVFEELM 495

Query: 561 RERFPKM----------------------------------VNHLDYLGVQVAWVTGPWF 620
           RERFPK+                                  VNHLDYLGVQVAW++GPWF
Sbjct: 496 RERFPKLGSLFSSDIQVSLHIFLPYTEQCDRFFYSNNPPDAVNHLDYLGVQVAWISGPWF 555

Query: 621 LSIFMNMLPWESVLRVWDVLLFEGNRVMLFRTALALMELYGPALVTTKDAGDAVTLLQSL 680
           LSIF+N++PWE VLR+WDVLLFEGNRV+LFRTA A+MELYGPA+V TKDAGDA+T LQSL
Sbjct: 556 LSIFVNIIPWECVLRMWDVLLFEGNRVVLFRTAFAIMELYGPAIVATKDAGDAITSLQSL 615

Query: 681 AGSTFDSSQLVLTACMGFQNVNETRLRELRTKHRPAVVTAIEERSKGLRAWKDSQGLASK 740
           A STFDSSQLVLTACMG+ + NE RL ELR  HRPAV+  +EER +  R WKD +GLASK
Sbjct: 616 ASSTFDSSQLVLTACMGYISTNEARLEELRKIHRPAVLEIVEERIQKGRVWKDKKGLASK 675

Query: 741 LYIFKHDSKSTITETKNSSQINGDLSRSESGSTNADEILISLAGEDEVDAVSNLQDQVVW 800
           LY FKH+      E K++ + +G+    +  S +    L     + EVD++ +LQ+QVVW
Sbjct: 676 LYSFKHEGSILDHEQKSTQRNDGENQDDDDESCSPFLNLDGANVDSEVDSLPDLQEQVVW 735

Query: 801 LKVELCKLLEEKRSAILRAEELETALMEMVTQDNRRQLSARVEQLEQEVAELQQALADKQ 860
           +KVELC+LLEEKRSA++RAEELE ALMEMV +DNR +LSAR+EQLE++V EL+Q L+DK+
Sbjct: 736 MKVELCRLLEEKRSAVMRAEELEIALMEMVKEDNRLELSARIEQLERDVRELKQVLSDKK 795

Query: 861 EQETAMLQVLMRVEQEQKLTEDARRFAEQDSTAQRYAAQMLQEKYEEAIVALAEMEKRAV 898
           EQETAMLQVLM+VEQ+QKLTEDAR  AEQD+ AQRYA  +LQEK E+ +  LA+MEK+ V
Sbjct: 796 EQETAMLQVLMKVEQDQKLTEDARINAEQDAAAQRYAVHVLQEKNEKLVTQLAQMEKKLV 855