Cp4.1LG01g04440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g04440 vs. ExPASy Swiss-Prot
Match: P25386 (Intracellular protein transport protein USO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=USO1 PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 8.5e-05
Identity = 132/641 (20.59%), Postives = 268/641 (41.81%), Query Frame = 0

Query: 222  RSYSSRETRSSLSRFLSEDGEYGFGNDSPRSAMTVVDRLSQQHERLVQ-------YSKFD 281
            + + S+E     SRF S D       +  +S       +  ++E L++        S   
Sbjct: 936  KEHISKELVEYKSRFQSHDNLVAKLTEKLKSLANNYKDMQAENESLIKAVEESKNESSIQ 995

Query: 282  VSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTE 341
            +S+L   I +++ E+    +E  S ++  I        +L Q  EE+ S++    K++ E
Sbjct: 996  LSNLQNKIDSMSQEKENFQIERGS-IEKNIEQLKKTISDLEQTKEEIISKSDS-SKDEYE 1055

Query: 342  LQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKS 401
             Q+ L KE          KLE      +    ++ EL +    L+ E+ +        K+
Sbjct: 1056 SQISLLKE----------KLETATTANDENVNKISELTKTREELEAELAAY-------KN 1115

Query: 402  MTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRE 461
            +   LE  +      + E  E  ++L+    +LE++   + + ++ +R N E  EKE  +
Sbjct: 1116 LKNELETKLETSEKALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHED 1175

Query: 462  LHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELAL 521
            L   + +       +E+  N    +L+++  + Q      +E E +K +   L G     
Sbjct: 1176 LAAQLKKYEEQIANKERQYNEEISQLNDEITSTQ------QENESIKKKNDELEGEV--- 1235

Query: 522  RKALESCRVEVDSLRRENINILT----HLKDNGNERGAT---TFKLVNEMSTRVYHLQNQ 581
             KA++S   E  +L++  I+ L      LK       A+   + K V   + ++  LQ++
Sbjct: 1236 -KAMKSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDE 1295

Query: 582  GMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDA-----HFFLESEAKIQGF 641
                  E ++     L+  ++K ++    +  +E I+  LDA        LE    +   
Sbjct: 1296 CNFKEKEVSEL-EDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKA 1355

Query: 642  KYGIESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLL 701
            K   ES    L+K S   +   N+      + N +Q+ +Q  E   +   +  +  +   
Sbjct: 1356 KEKSESELSRLKKTSS--EERKNAEEQLEKLKNEIQIKNQAFEKERKLLNEGSSTITQEY 1415

Query: 702  KEKLFSKELEVEQLQAELATAVRGNDMLKCEVQ----NGMEGLSCLSHKIKDLELQLLKR 761
             EK+ + E E+ +LQ E     +  D  + E++    +  E L    + IK L+ ++L  
Sbjct: 1416 SEKINTLEDELIRLQNENELKAKEIDNTRSELEKVSLSNDELLEEKQNTIKSLQDEILSY 1475

Query: 762  NEDINKLQNEL----EESRRELEILRDVLQKISKEKDMLWEEVNKHREKNMLLISKVDEL 821
             + I +   +L     +++R+LE L++ L+   + K  + E + K  E++    +++++ 
Sbjct: 1476 KDKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKS 1535

Query: 822  KSKIETLEEDILLKEGQITILKDTLTNKSIDLLASPKSSWE 836
            K  ++ LE  I   E ++    +T+      L  S KS+ E
Sbjct: 1536 KEMMKKLESTIESNETELKSSMETIRKSDEKLEQSKKSAEE 1544

BLAST of Cp4.1LG01g04440 vs. ExPASy Swiss-Prot
Match: A3KNA5 (Filamin A-interacting protein 1-like OS=Danio rerio OX=7955 GN=filip1l PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.1e-04
Identity = 130/586 (22.18%), Postives = 239/586 (40.78%), Query Frame = 0

Query: 311 REELRQANEELESRTQKLEKEKTELQVGLEKELDRRSSDWSFKL----EKYKLEEE--GQ 370
           RE L+Q  ++ ++  ++ E+E  +    L+ EL +     SF L    E+ +L E+   Q
Sbjct: 192 RERLKQLIDQEKTYQERKEEESNKQVTNLKDELTKLK---SFALLVVDEQQRLTEQLSQQ 251

Query: 371 RGRVRELAEQNVSLQREVVSLNKR-----------ETENKSMTTNLEQNILDLTARIDEK 430
             +V+EL       Q E+ S   R           E E +  T +  Q    +TA++  +
Sbjct: 252 ITKVQELQNSAGQAQEELNSAQTRLQDEENKVLRLEEELRKQTCHFHQEQDAMTAKLTNE 311

Query: 431 NEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCNEQEKTI 490
           + QN+ L+  LS L       ++ ++ I K     E+E +EL   I+R     +     +
Sbjct: 312 DAQNRQLRQKLSSLSR----QLDELEEINKTLHRAEEELQELRDKISRGECGNSSLVSEV 371

Query: 491 NGLRERLSEQFGNIQPMEKLD---------------------KEFEKLKMEQMRLTGVEL 550
             LR+R+ E  G  + + K++                      E +KL    M L  +E 
Sbjct: 372 EELRKRVLEMEGKDEELIKMEDMCRELNRKLEKEAKQSCSLKAEVDKLNHRIMELEKLED 431

Query: 551 ALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLL 610
           A RK+ + C     +L +E     T  K   NE      + + E+ T   HL+   + L 
Sbjct: 432 AFRKSKQECNSLKSNLEKER----TMTKHMSNELDVLRVR-IKELETTEVHLEKTELTLK 491

Query: 611 NESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMS 670
            + T+  +  +  + E+ A     K   + ++N         S  K+Q  +  + ++T  
Sbjct: 492 EDLTKLKTLTVMLVDERKAMAEKLKMMEDKVQN---------STGKLQAEQDKVNTVTEK 551

Query: 671 LQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEK--LFSKE 730
           L +       ES  T  S          +    D L+++LK E   SS L+ K  +  K 
Sbjct: 552 LIE-------ESKKTLRSKAELEEKMCVATRERDELKAKLKTEEEKSSDLQSKVSMMKKR 611

Query: 731 LE-VEQLQAELATAVRGNDMLKC-----------EVQNGMEGLSCLSHKIKDLEL---QL 790
           L+ +E ++ EL       +  K            +V++  + +  L  K+K++++    L
Sbjct: 612 LQSLETVERELLRNKSKEEHTKSPGPYRYQQEDNKVKDLTQEVERLRRKLKEMKVVEGDL 671

Query: 791 LKRNEDINKLQ--------------NELEESRRELEILRDVLQKISKEKDMLWEEVNKHR 825
           LK  ++   L+               ELE SR+EL   +   ++ S ++ +L++ + +  
Sbjct: 672 LKTEDEFESLEKRYSNEQERAKALMEELETSRKELSKYQLAEKEESNQEQILYKRLKEEE 731

BLAST of Cp4.1LG01g04440 vs. ExPASy Swiss-Prot
Match: D3ZZL9 (GRIP and coiled-coil domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Gcc2 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 7.2e-04
Identity = 125/604 (20.70%), Postives = 256/604 (42.38%), Query Frame = 0

Query: 259  RLSQQHERLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQAN 318
            ++SQ+ E + Q    DV +L Q ++    E+ AL LE  + LQ    ++  ++E +    
Sbjct: 495  KISQEFETMKQQQASDVHELQQKLRTAFNEKDAL-LETINRLQGE-NEKLLSQELV---- 554

Query: 319  EELESRTQKLEKEKTELQVGLE------KELDRRSSDWSFKLEKYKLEEEGQRGRVRELA 378
             ELES  + L+ + +     L       +EL+ + S  + + + +  + +  R  + +L 
Sbjct: 555  SELESTMKNLKADNSMYLASLGQKDTLLQELEAKISSLAEEKDDFISKIKTSREEIDDLH 614

Query: 379  EQNVSLQREVVSLNKRETENKSMTTNLEQNILDLTARID----EKNEQNKYLQLNLSKLE 438
            ++    Q+  V L +   +     + L Q + +LT ++D    EK++ ++ + + +  + 
Sbjct: 615  QKWEREQKLSVELREAAEQAAQHNSELRQRVSELTGKLDEILREKSQNDQNIMVQMKTMT 674

Query: 439  EDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCNE-QEKTINGLRERLSEQFGNI 498
            ED        + +    +   +E   LH    +L+R     Q +     +E ++E    +
Sbjct: 675  ED-------QEALSSKIKSLYEENNRLHSEKVQLSRDLEALQSQQDFAYKEHVAEFEKKL 734

Query: 499  QPM-EKLDKEFEKLKMEQMRLTGVELALRKALESCRVEV--DSLRRENINILTHLKDNGN 558
            Q M E+ D   + L+ EQ++ + V+  L + L+  R  +  D+   + + +L  + ++  
Sbjct: 735  QLMVEERDDLNKLLENEQLQKSFVKTQLYEFLKQMRPSILEDNEEEDVVTVLKAVGESLV 794

Query: 559  ERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIE 618
                    LV E   RV  L+ +   L  ES   C +L   +++   +    +   + + 
Sbjct: 795  TVKEEKHNLVFEYDARVLELERRIKCLQEESVVQCEELRALVRDSEQEKILLRKELDEVT 854

Query: 619  NGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYS 678
            +  +A      +  I   K   E  ++  Q +S  ++  S S    + V N   L  ++ 
Sbjct: 855  STKEA-----LQCDILEMKNTNEKTSLENQTLSTRVEELSRSLHSKNEVHNEKDLVIEHE 914

Query: 679  EDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCL 738
                             L+  L  +E E++ ++AEL       +       + +  +  L
Sbjct: 915  N----------------LRLSLEQRESELQDVRAELMLLKDSLEKSPSVKNDQLSLVKEL 974

Query: 739  SHKIKDLELQLLKRNEDINKL-------QNELEESRRELEILRDVLQKISKEKDMLWEEV 798
              KI+ LE +   ++E I+K+       + EL+ +R+E + LRD L+ +  EKD L   +
Sbjct: 975  EEKIESLEKESKDKDEKISKIKLVAVRAKKELDSNRKEAQTLRDELESVQSEKDRLSASM 1034

Query: 799  NKHRE-----KNMLL-----ISKVDELKSKIETLE---EDILLKEGQITILKDTLTNKSI 829
             +  +     KN+LL       ++D  K +    E   ED+  +    T   + LT+ + 
Sbjct: 1035 KEFIQGAESYKNLLLEYDKQSEQLDVEKERANNFEHHIEDLTKQLRDSTCQYEKLTSDNE 1064

BLAST of Cp4.1LG01g04440 vs. NCBI nr
Match: XP_023528803.1 (protein Daple-like [Cucurbita pepo subsp. pepo] >XP_023528811.1 protein Daple-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1545 bits (4000), Expect = 0.0
Identity = 839/912 (92.00%), Postives = 839/912 (92.00%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS
Sbjct: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG 180
           SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG 180

Query: 181 EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240
           EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED
Sbjct: 181 EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240

Query: 241 GEYGFGNDSPRSAMTVVDRLSQQH------------------------------------ 300
           GEYGFGNDSPRSAMTVVDRLSQQH                                    
Sbjct: 241 GEYGFGNDSPRSAMTVVDRLSQQHVVPRGSYKELGENIPITVADTYSRSLNGCFDPNADL 300

Query: 301 -------------------------------------ERLVQYSKFDVSDLIQIIKNLTG 360
                                                ERLVQYSKFDVSDLIQIIKNLTG
Sbjct: 301 TKPCFPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLTG 360

Query: 361 ERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420
           ERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS
Sbjct: 361 ERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420

Query: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLT 480
           SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLT
Sbjct: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLT 480

Query: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCN 540
           ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCN
Sbjct: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCN 540

Query: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600
           EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS
Sbjct: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600

Query: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660
           LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK
Sbjct: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660

Query: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNST 720
           EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNST
Sbjct: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNST 720

Query: 721 SPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGN 780
           SPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGN
Sbjct: 721 SPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGN 780

Query: 781 DMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKIS 839
           DMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKIS
Sbjct: 781 DMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKIS 840

BLAST of Cp4.1LG01g04440 vs. NCBI nr
Match: XP_022941780.1 (golgin subfamily B member 1-like [Cucurbita moschata] >XP_022941781.1 golgin subfamily B member 1-like [Cucurbita moschata])

HSP 1 Score: 1504 bits (3895), Expect = 0.0
Identity = 823/913 (90.14%), Postives = 827/913 (90.58%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGT IGDKAGSSPQRSKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTPIGDKAGSSPQRSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           F+RQIDDNERS SRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRS GNASSRSKRQHEQS
Sbjct: 61  FNRQIDDNERSRSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           SRCQSPSREMQFKVKQTELPNDY TSGS RPCSRT YDSSGNSTT+SSIVSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYYTSGSFRPCSRTWYDSSGNSTTTSSIVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240
           GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE
Sbjct: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240

Query: 241 DGEYGFGNDSPRSAMTVVDRLSQQH----------------------------------- 300
           DGEYGFGNDSPRSA TVVDRLSQQH                                   
Sbjct: 241 DGEYGFGNDSPRSAKTVVDRLSQQHVVPRGSYKELGENIPITVADTYSRSLNGCFDPNAD 300

Query: 301 --------------------------------------ERLVQYSKFDVSDLIQIIKNLT 360
                                                 ERLVQYSKFDVSDLIQIIKNLT
Sbjct: 301 LTKPCIPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLT 360

Query: 361 GERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420
           GERF LALEVSSLLQSRI DRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR
Sbjct: 361 GERFTLALEVSSLLQSRIADRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420

Query: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDL 480
           SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREV SLNKRETENKSMTTNLEQNILDL
Sbjct: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASLNKRETENKSMTTNLEQNILDL 480

Query: 481 TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540
           TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC
Sbjct: 481 TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540

Query: 541 NEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD 600
           NEQEKTINGLRERLSEQFGN QPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD
Sbjct: 541 NEQEKTINGLRERLSEQFGNSQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD 600

Query: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660
           SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI
Sbjct: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660

Query: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720
           KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS
Sbjct: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720

Query: 721 TSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRG 780
           TS SSGVDNALQLNSQYSEDGLRSELKAETLFSSLL+EKLFSKELEVEQLQAELATAVRG
Sbjct: 721 TSQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREKLFSKELEVEQLQAELATAVRG 780

Query: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKI 839
           NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQ ELEESRRELEILRDVLQKI
Sbjct: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQTELEESRRELEILRDVLQKI 840

BLAST of Cp4.1LG01g04440 vs. NCBI nr
Match: XP_022991729.1 (protein Daple-like [Cucurbita maxima] >XP_022991791.1 protein Daple-like [Cucurbita maxima] >XP_022991861.1 protein Daple-like [Cucurbita maxima])

HSP 1 Score: 1500 bits (3884), Expect = 0.0
Identity = 818/912 (89.69%), Postives = 824/912 (90.35%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQ SKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQSSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           FDRQIDDNERS SRPKLRRTQSLSSAAFRDQG+INFDGLIDPSRS GNASSRSKRQHEQS
Sbjct: 61  FDRQIDDNERSRSRPKLRRTQSLSSAAFRDQGRINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG 180
           SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGN TTSSSIVSN VLDRYIDG
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNCTTSSSIVSNMVLDRYIDG 180

Query: 181 EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240
           EQHQEINGSKNKY QRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED
Sbjct: 181 EQHQEINGSKNKYYQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240

Query: 241 GEYGFGNDSPRSAMTVVDRLSQQH------------------------------------ 300
           GEYGFGN+SPRSA TVVDRLSQQ                                     
Sbjct: 241 GEYGFGNESPRSAKTVVDRLSQQRVVPRGSYKELGENIPITVADTYSRSLNGCFDPNADL 300

Query: 301 -------------------------------------ERLVQYSKFDVSDLIQIIKNLTG 360
                                                ERLVQYSKFDVSDLIQIIKNLTG
Sbjct: 301 TKPCFPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLTG 360

Query: 361 ERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420
           ERF LALEVSSLLQSRI DRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS
Sbjct: 361 ERFTLALEVSSLLQSRIADRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420

Query: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLT 480
           SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREV SLNKRETENKSMTTNLE NILDLT
Sbjct: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASLNKRETENKSMTTNLETNILDLT 480

Query: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCN 540
           ARIDEKNEQNKYLQLNLSKLEEDYRGSIEG+DCIRKNFEEKEKECRELHKSITRLTRTCN
Sbjct: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGIDCIRKNFEEKEKECRELHKSITRLTRTCN 540

Query: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600
           EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS
Sbjct: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600

Query: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660
           LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK
Sbjct: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660

Query: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNST 720
           EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 
Sbjct: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNSN 720

Query: 721 SPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGN 780
           S SSGVDNALQLNSQYSEDGLRSELKAETLFSSLL+EKLFSKELEVEQLQAELATAVRGN
Sbjct: 721 SQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREKLFSKELEVEQLQAELATAVRGN 780

Query: 781 DMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKIS 839
           D+LKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEES RELEILRDVLQKIS
Sbjct: 781 DVLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESTRELEILRDVLQKIS 840

BLAST of Cp4.1LG01g04440 vs. NCBI nr
Match: KAG7030758.1 (hypothetical protein SDJN02_04795, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1494 bits (3867), Expect = 0.0
Identity = 817/913 (89.49%), Postives = 824/913 (90.25%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGT IGDKAGSSPQ SKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTPIGDKAGSSPQSSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           F+RQIDDNERS SRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRS GNASSRSKRQHEQS
Sbjct: 61  FNRQIDDNERSRSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           SRCQSPSREMQFKVKQTELPNDY TSGSVRP SRTCYDSSGNSTT+SSIVSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYYTSGSVRPSSRTCYDSSGNSTTTSSIVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240
           GEQHQEINGSKNKYSQRNNGWRPPRAQ LPPSSTTASIKDNPRSYSSRETRSSLSRFLSE
Sbjct: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQYLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240

Query: 241 DGEYGFGNDSPRSAMTVVDRLSQQH----------------------------------- 300
           DGEYGFGNDSPRSA T VDRLSQQH                                   
Sbjct: 241 DGEYGFGNDSPRSAKTAVDRLSQQHVVPRGSYKELGENIPITVADTYSRSLNGCFDHNAD 300

Query: 301 --------------------------------------ERLVQYSKFDVSDLIQIIKNLT 360
                                                 ERLVQYSKFDVSDLIQIIKNLT
Sbjct: 301 LTKPCIPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLT 360

Query: 361 GERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420
           GERF LALEVSSLLQSRI DRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR
Sbjct: 361 GERFTLALEVSSLLQSRIADRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420

Query: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDL 480
           SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREV SLNKRETENKSMTTNLEQN+LDL
Sbjct: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASLNKRETENKSMTTNLEQNVLDL 480

Query: 481 TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540
           TARIDEKNEQNKYLQLNLSKLEED+RGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC
Sbjct: 481 TARIDEKNEQNKYLQLNLSKLEEDFRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540

Query: 541 NEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD 600
           NEQEKTINGLRERLSEQFGN QPMEKLDKEFEKLKMEQMRLTGVELALRKALESCR EVD
Sbjct: 541 NEQEKTINGLRERLSEQFGNSQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRAEVD 600

Query: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660
           SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI
Sbjct: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660

Query: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720
           KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS
Sbjct: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720

Query: 721 TSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRG 780
           TS SSGVDNALQLNSQYSEDGLRSELKAETLFSSLL+EKLFSKELEVEQLQAELATAVRG
Sbjct: 721 TSQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREKLFSKELEVEQLQAELATAVRG 780

Query: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKI 839
           NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQ ELEESRRELEILRDVLQKI
Sbjct: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQTELEESRRELEILRDVLQKI 840

BLAST of Cp4.1LG01g04440 vs. NCBI nr
Match: XP_038893371.1 (rho-associated protein kinase 1 isoform X1 [Benincasa hispida] >XP_038893372.1 rho-associated protein kinase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1192 bits (3083), Expect = 0.0
Identity = 681/934 (72.91%), Postives = 741/934 (79.34%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKK F RSFG G GK++   PS T++SE + EHPL  R  +SI DKAGSSPQ ++     
Sbjct: 1   MKKLFFRSFGTGNGKHNLALPS-TNESETHLEHPLEGRKSSSISDKAGSSPQSTRS---- 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
             +QIDD+ERS + PKLRRT+SLSSAAFRDQGQI+F G  DPSRS GNASS  KRQHEQS
Sbjct: 61  -GKQIDDSERSSTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNASSGFKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           SRCQSPSREMQFK KQ E+P+DY TSG VRPCSRTCYDSSGNS+ S S VSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFKAKQLEMPHDYYTSGPVRPCSRTCYDSSGNSSNSVSSVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLS- 240
           GEQHQEINGS NK  QRNNGWRPPRAQCL  +ST+ASIKD PRSYSSRE +SS SR LS 
Sbjct: 181 GEQHQEINGSMNKCFQRNNGWRPPRAQCLLHASTSASIKDKPRSYSSREAKSSHSRLLSG 240

Query: 241 EDGEYGFGNDSPRS-AMTVVDRLSQQH--------------------------------- 300
           E  EYGFGNDSPRS A  VVDRLSQ H                                 
Sbjct: 241 EVVEYGFGNDSPRSIAKNVVDRLSQHHVVPKATSKELDENIPITVTDIHSRSSNGCFDPN 300

Query: 301 -----------------------------------------------------------E 360
                                                                      E
Sbjct: 301 SDLATQPCFPTDEPWETVSGHIYESCKPGETNEDFDGELQKRAKEAEERVVFLSEELEQE 360

Query: 361 RLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRT 420
           R  QY KFDVSDLIQIIKNL GERF LALE+S+LLQSRI DRTCAR+ELRQAN ELESRT
Sbjct: 361 RFNQYRKFDVSDLIQIIKNLNGERFTLALEISNLLQSRIADRTCARKELRQANAELESRT 420

Query: 421 QKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSL 480
            KLEKEK ELQVGLEKELDRRSSDWSFKLEKY+LEEEG RGRVRELAEQNVSLQREV SL
Sbjct: 421 LKLEKEKIELQVGLEKELDRRSSDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSL 480

Query: 481 NKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNF 540
           NK ETEN+S+TTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRG+IEGMDCIRKNF
Sbjct: 481 NKMETENRSITTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGAIEGMDCIRKNF 540

Query: 541 EEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQM 600
           EEKEKEC ELHKSITRL RTCNEQEKTI+GLRERLSEQFGNIQP+EKLDK+FE+LKMEQM
Sbjct: 541 EEKEKECGELHKSITRLLRTCNEQEKTIDGLRERLSEQFGNIQPVEKLDKQFERLKMEQM 600

Query: 601 RLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQ 660
           RLTGVELALRK LES RVEVDSLR ENI ILT LK+NGNE GA TFKL NEMS RVYHLQ
Sbjct: 601 RLTGVELALRKELESYRVEVDSLRHENIKILTRLKENGNESGAITFKLDNEMSARVYHLQ 660

Query: 661 NQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYG 720
           NQG+VLLNESTQFCS+LLEFIKEK  Q HP KHR E+I+NGL   FFLESE KI+ FK+G
Sbjct: 661 NQGLVLLNESTQFCSKLLEFIKEKVGQFHPTKHRIEYIKNGLYGQFFLESEMKIRSFKHG 720

Query: 721 IESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEK 780
           IESLTMSLQKISMLLQA+SNSTS SSGVDNALQL+ QY+EDGLRSELKAETLFSSLL+EK
Sbjct: 721 IESLTMSLQKISMLLQAKSNSTSQSSGVDNALQLSCQYAEDGLRSELKAETLFSSLLREK 780

Query: 781 LFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKL 839
           L+SKELE+EQLQAEL TAVRGND+LKCEVQNGM+ LSCL+HK+KDLELQLLK+NEDINKL
Sbjct: 781 LYSKELEMEQLQAELVTAVRGNDILKCEVQNGMDSLSCLTHKMKDLELQLLKKNEDINKL 840

BLAST of Cp4.1LG01g04440 vs. ExPASy TrEMBL
Match: A0A6J1FM18 (golgin subfamily B member 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447046 PE=4 SV=1)

HSP 1 Score: 1504 bits (3895), Expect = 0.0
Identity = 823/913 (90.14%), Postives = 827/913 (90.58%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGT IGDKAGSSPQRSKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTPIGDKAGSSPQRSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           F+RQIDDNERS SRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRS GNASSRSKRQHEQS
Sbjct: 61  FNRQIDDNERSRSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           SRCQSPSREMQFKVKQTELPNDY TSGS RPCSRT YDSSGNSTT+SSIVSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYYTSGSFRPCSRTWYDSSGNSTTTSSIVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240
           GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE
Sbjct: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240

Query: 241 DGEYGFGNDSPRSAMTVVDRLSQQH----------------------------------- 300
           DGEYGFGNDSPRSA TVVDRLSQQH                                   
Sbjct: 241 DGEYGFGNDSPRSAKTVVDRLSQQHVVPRGSYKELGENIPITVADTYSRSLNGCFDPNAD 300

Query: 301 --------------------------------------ERLVQYSKFDVSDLIQIIKNLT 360
                                                 ERLVQYSKFDVSDLIQIIKNLT
Sbjct: 301 LTKPCIPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLT 360

Query: 361 GERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420
           GERF LALEVSSLLQSRI DRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR
Sbjct: 361 GERFTLALEVSSLLQSRIADRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRR 420

Query: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDL 480
           SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREV SLNKRETENKSMTTNLEQNILDL
Sbjct: 421 SSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASLNKRETENKSMTTNLEQNILDL 480

Query: 481 TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540
           TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC
Sbjct: 481 TARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTC 540

Query: 541 NEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD 600
           NEQEKTINGLRERLSEQFGN QPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD
Sbjct: 541 NEQEKTINGLRERLSEQFGNSQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVD 600

Query: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660
           SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI
Sbjct: 601 SLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFI 660

Query: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720
           KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS
Sbjct: 661 KEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 720

Query: 721 TSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRG 780
           TS SSGVDNALQLNSQYSEDGLRSELKAETLFSSLL+EKLFSKELEVEQLQAELATAVRG
Sbjct: 721 TSQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREKLFSKELEVEQLQAELATAVRG 780

Query: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKI 839
           NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQ ELEESRRELEILRDVLQKI
Sbjct: 781 NDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQTELEESRRELEILRDVLQKI 840

BLAST of Cp4.1LG01g04440 vs. ExPASy TrEMBL
Match: A0A6J1JRR9 (protein Daple-like OS=Cucurbita maxima OX=3661 GN=LOC111488230 PE=4 SV=1)

HSP 1 Score: 1500 bits (3884), Expect = 0.0
Identity = 818/912 (89.69%), Postives = 824/912 (90.35%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQ SKDLPSK
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQSSKDLPSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
           FDRQIDDNERS SRPKLRRTQSLSSAAFRDQG+INFDGLIDPSRS GNASSRSKRQHEQS
Sbjct: 61  FDRQIDDNERSRSRPKLRRTQSLSSAAFRDQGRINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG 180
           SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGN TTSSSIVSN VLDRYIDG
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNCTTSSSIVSNMVLDRYIDG 180

Query: 181 EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240
           EQHQEINGSKNKY QRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED
Sbjct: 181 EQHQEINGSKNKYYQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240

Query: 241 GEYGFGNDSPRSAMTVVDRLSQQH------------------------------------ 300
           GEYGFGN+SPRSA TVVDRLSQQ                                     
Sbjct: 241 GEYGFGNESPRSAKTVVDRLSQQRVVPRGSYKELGENIPITVADTYSRSLNGCFDPNADL 300

Query: 301 -------------------------------------ERLVQYSKFDVSDLIQIIKNLTG 360
                                                ERLVQYSKFDVSDLIQIIKNLTG
Sbjct: 301 TKPCFPTDEPGETDGELQKKAKEAEERIMFLSEELEQERLVQYSKFDVSDLIQIIKNLTG 360

Query: 361 ERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420
           ERF LALEVSSLLQSRI DRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS
Sbjct: 361 ERFTLALEVSSLLQSRIADRTCAREELRQANEELESRTQKLEKEKTELQVGLEKELDRRS 420

Query: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLT 480
           SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREV SLNKRETENKSMTTNLE NILDLT
Sbjct: 421 SDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASLNKRETENKSMTTNLETNILDLT 480

Query: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCN 540
           ARIDEKNEQNKYLQLNLSKLEEDYRGSIEG+DCIRKNFEEKEKECRELHKSITRLTRTCN
Sbjct: 481 ARIDEKNEQNKYLQLNLSKLEEDYRGSIEGIDCIRKNFEEKEKECRELHKSITRLTRTCN 540

Query: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600
           EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS
Sbjct: 541 EQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDS 600

Query: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660
           LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK
Sbjct: 601 LRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIK 660

Query: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNST 720
           EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNS 
Sbjct: 661 EKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNSN 720

Query: 721 SPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGN 780
           S SSGVDNALQLNSQYSEDGLRSELKAETLFSSLL+EKLFSKELEVEQLQAELATAVRGN
Sbjct: 721 SQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREKLFSKELEVEQLQAELATAVRGN 780

Query: 781 DMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESRRELEILRDVLQKIS 839
           D+LKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEES RELEILRDVLQKIS
Sbjct: 781 DVLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEESTRELEILRDVLQKIS 840

BLAST of Cp4.1LG01g04440 vs. ExPASy TrEMBL
Match: A0A0A0KNP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177090 PE=4 SV=1)

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 661/934 (70.77%), Postives = 739/934 (79.12%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKK FLRSFG G+GKN+S  PS T++SE +WE+PL SR  +S   KAGSSPQ ++     
Sbjct: 1   MKKLFLRSFGTGHGKNNSAIPS-TNESETHWENPLESRTSSS---KAGSSPQSTRS---- 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
             + IDD+ER  + PKLRRT+SLSSAAFRDQGQI+F G  DPSRS GN+SS  KRQHE S
Sbjct: 61  -GKHIDDSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           SRCQSPSREMQF  KQ E+PNDY  SGS+RP SRTCYDSSGNS+TS S VSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240
           GEQHQEINGS +K SQR+NGWRPPRAQCLP +STTASIKD PRSYSSRE + S+SR LSE
Sbjct: 181 GEQHQEINGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSE 240

Query: 241 D-GEYGFGNDSPRS-AMTVVDRLSQQH--------------------------------- 300
           + GEYGFGNDSPRS A TVVD+LSQ H                                 
Sbjct: 241 EVGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPN 300

Query: 301 -----------------------------------------------------------E 360
                                                                      E
Sbjct: 301 SDLGNQPCFPTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQE 360

Query: 361 RLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRT 420
           R  QY KFDVSDLIQII+ LTGERF LALE+S+LLQSRI DRTCAREELRQAN ELESRT
Sbjct: 361 RFNQYRKFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRT 420

Query: 421 QKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSL 480
           QKLEKEK ELQVGLEKELDRRS+DWSFKLEKY+LEEEG RGRVRELAEQNVSLQREV SL
Sbjct: 421 QKLEKEKIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSL 480

Query: 481 NKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNF 540
           NK ETEN+++TTNLEQNI+DLTA+IDEKNE+NKYLQLNLSKLEEDYRG+IEGMDCIRKN+
Sbjct: 481 NKMETENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNY 540

Query: 541 EEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQM 600
           EEKEKEC++LHKSITRL+RTCNEQEKTI+GLRERLSEQF NIQP+EK DK+ E+LKMEQM
Sbjct: 541 EEKEKECKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQM 600

Query: 601 RLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQ 660
           RLTGVELALRK LESCRVEVDSLR ENI ILT LKDNGNE GA TFKL NEMS RVYHLQ
Sbjct: 601 RLTGVELALRKELESCRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQ 660

Query: 661 NQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYG 720
           NQG+VLLNESTQFCS+LLEFIKEK  Q HP +HR EHI+NGLD  FFLESE KI+  K+G
Sbjct: 661 NQGLVLLNESTQFCSKLLEFIKEKIGQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHG 720

Query: 721 IESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEK 780
           IESLTMSLQKISMLLQA+SN TS +S VDNALQLN QY EDGLRSELKAETLFSSLL+EK
Sbjct: 721 IESLTMSLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREK 780

Query: 781 LFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKL 839
           L+SKELEVEQLQ EL TAVRGND+LKCEVQNGM+GLSCL+HK+KDLELQL  +NE+I+KL
Sbjct: 781 LYSKELEVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL 840

BLAST of Cp4.1LG01g04440 vs. ExPASy TrEMBL
Match: A0A6J1CAU5 (myosin-13 OS=Momordica charantia OX=3673 GN=LOC111009977 PE=4 SV=1)

HSP 1 Score: 1155 bits (2987), Expect = 0.0
Identity = 658/946 (69.56%), Postives = 722/946 (76.32%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKKFF RS  N       + P ST+D +AYWEHPL SRM  SIGDKAGSSPQ +K   SK
Sbjct: 1   MKKFFFRSKHN-------IAPPSTNDCKAYWEHPLESRMNNSIGDKAGSSPQSTKHFSSK 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
              Q +D ERS + PKLRRT+SLSSAAF DQGQ+NF G  DPSRS G     S+RQHEQS
Sbjct: 61  SGMQTNDIERSSTSPKLRRTRSLSSAAFGDQGQMNFYGPSDPSRSPG-----SQRQHEQS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYIDG 180
           SRCQ P+ EMQFKVKQ E+PNDY +G VRPCS+TCYDSSGNS+TSSS VSNRVLDRYIDG
Sbjct: 121 SRCQGPTWEMQFKVKQMEVPNDYYTGPVRPCSKTCYDSSGNSSTSSSNVSNRVLDRYIDG 180

Query: 181 EQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSED 240
           EQHQEI+GSKNKYSQ+NNGWRPPRAQCL PSS TASIKD PRSYSSRE +SS SRF S +
Sbjct: 181 EQHQEISGSKNKYSQKNNGWRPPRAQCLTPSSPTASIKDKPRSYSSREVKSSHSRFSSRE 240

Query: 241 -GEYGFGNDSPRS-AMTVVDRLSQQH---------------------------------- 300
            GEYGFGN+SPRS A  VVDRLSQ H                                  
Sbjct: 241 LGEYGFGNESPRSIAKNVVDRLSQYHVLPKATSKELGENVPIRTTDIQNQSLNGCYDPNL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 DVVTRPCFPTDEPCETVSGPHTEELSGFHKQKISPGRMYEGCKSGETDGDLDGELQRSVK 360

Query: 361 ---------------ERLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTC 420
                          ER VQY KFDVSDLIQ+IKNL+ +RF LALE+SSLLQSRI DR  
Sbjct: 361 EADERIMFLSEELEQERFVQYRKFDVSDLIQVIKNLSEDRFTLALEISSLLQSRIADRES 420

Query: 421 AREELRQANEELESRTQKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVR 480
           A+EELRQAN EL+SRT+KLEKEKTELQ+GLEKELDRRSSDWSFKLEKYKLEE+G RGRVR
Sbjct: 421 AKEELRQANAELDSRTRKLEKEKTELQLGLEKELDRRSSDWSFKLEKYKLEEQGLRGRVR 480

Query: 481 ELAEQNVSLQREVVSLNKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEE 540
           ELAEQNVSLQREV SLNK ETENKS  TNLEQN+LDLT RIDEKNEQN YLQLNLSKLEE
Sbjct: 481 ELAEQNVSLQREVSSLNKTETENKSTITNLEQNVLDLTTRIDEKNEQNNYLQLNLSKLEE 540

Query: 541 DYRGSIEGMDCIRKNFEEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQP 600
           DYRG+ EGMDCIRKNFEEKEKECRELHKSITRL+RTC+EQEKTI+GLRERLSEQF NIQP
Sbjct: 541 DYRGATEGMDCIRKNFEEKEKECRELHKSITRLSRTCSEQEKTIDGLRERLSEQFVNIQP 600

Query: 601 MEKLDKEFEKLKMEQMRLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGAT 660
           +EK DK FEKLKMEQMRLTGVE+ALRK LES RVEVDSLR ENINILT LKDNGNE GA 
Sbjct: 601 VEKFDKHFEKLKMEQMRLTGVEMALRKELESYRVEVDSLRHENINILTRLKDNGNESGAI 660

Query: 661 TFKLVNEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDA 720
            FKL NEMS+RVYHLQNQG+VLL ESTQFCS+LLEFIKEK  QL   KHR EH++NGLD 
Sbjct: 661 NFKLDNEMSSRVYHLQNQGLVLLKESTQFCSKLLEFIKEKVGQLQQTKHRMEHMKNGLDG 720

Query: 721 HFFLESEAKIQGFKYGIESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLR 780
            FF+ESE KIQGFK+GIESLTMSL + SM+LQA+SN TS SSGVDNALQ+NSQY ED LR
Sbjct: 721 QFFVESETKIQGFKHGIESLTMSLHRTSMVLQAKSNPTSQSSGVDNALQINSQYPEDVLR 780

Query: 781 SELKAETLFSSLLKEKLFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIK 835
           SELKAETL +SLL+EKL+SKELEVEQLQAEL TAVRGND+LKCEVQN M+ LSCL+HK+K
Sbjct: 781 SELKAETLLTSLLREKLYSKELEVEQLQAELVTAVRGNDILKCEVQNQMDSLSCLTHKMK 840

BLAST of Cp4.1LG01g04440 vs. ExPASy TrEMBL
Match: A0A1S3BDK7 (rho-associated protein kinase 1 OS=Cucumis melo OX=3656 GN=LOC103488729 PE=4 SV=1)

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 654/934 (70.02%), Postives = 732/934 (78.37%), Query Frame = 0

Query: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSK 60
           MKK FLRSFG G+GKN+S  PS T++S+ +WEHP  SR  +S   KAGSSPQ ++     
Sbjct: 1   MKKLFLRSFGTGHGKNNSALPS-TNESQTHWEHPFESRTSSS---KAGSSPQSTRS---- 60

Query: 61  FDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHEQS 120
             + IDD+ER    PKLRRT+SLSSAAFRDQGQ++F G  DPSR+ GN+SS  K+Q E S
Sbjct: 61  -GKHIDDSERFGMGPKLRRTRSLSSAAFRDQGQVDFYGSSDPSRTPGNSSSGFKQQCEPS 120

Query: 121 SRCQSPSREMQFKVKQTELPNDY-TSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 180
           S CQSPSREMQFK KQ E+PNDY TSG VRP SR CYDSSGNS+TS S VSNRVLDRYID
Sbjct: 121 SCCQSPSREMQFKTKQMEMPNDYYTSGFVRPSSRACYDSSGNSSTSVSSVSNRVLDRYID 180

Query: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240
           GEQHQEINGS NK SQRNNGWRPPRAQCLP +STTASIKD PRSYSSRE + S+S  LSE
Sbjct: 181 GEQHQEINGSMNKCSQRNNGWRPPRAQCLPHTSTTASIKDKPRSYSSREAKGSISHLLSE 240

Query: 241 D-GEYGFGNDSPRS-AMTVVDRLSQQH--------------------------------- 300
           + GEYGFGNDSP+S A TVVDRLSQ H                                 
Sbjct: 241 EVGEYGFGNDSPQSIAKTVVDRLSQHHVVPKANSRELGENVPITVTDIHTRSSNGCFDPN 300

Query: 301 -----------------------------------------------------------E 360
                                                                      E
Sbjct: 301 SDLANQPCFPTDAPWKTVSEHMYETCKPSETNEDFDGELQKRAKEAEERVMYLSEELEQE 360

Query: 361 RLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRT 420
           R  QY KFDVSDLIQIIK LTGERF  ALE+S+LLQSRI DRTCAREELRQAN ELESRT
Sbjct: 361 RFNQYRKFDVSDLIQIIKILTGERFTFALEISNLLQSRIADRTCAREELRQANAELESRT 420

Query: 421 QKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSL 480
           QKLEKEK ELQVGLEKELDRRS+DWSFKLEKY+LEEEG RGRVRELAEQNVSLQREV SL
Sbjct: 421 QKLEKEKIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSL 480

Query: 481 NKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNF 540
           NK  TEN+++TTNLEQNI+DLTA+IDEKNE+NKYLQ+NLSKLEEDYRG+IEGMDCIRKN+
Sbjct: 481 NKMVTENRTITTNLEQNIVDLTAKIDEKNEENKYLQINLSKLEEDYRGAIEGMDCIRKNY 540

Query: 541 EEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQM 600
           EEKEKEC++LHKSITRL+RTCNEQEKTI+GLRERLSEQF NIQP+EK DK+FE+LK+EQM
Sbjct: 541 EEKEKECKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQFERLKIEQM 600

Query: 601 RLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQ 660
           RLTGVELALRK LESCRVEVDSLRRENI ILT LKDNGNE GA TFKL NEMS RVYHLQ
Sbjct: 601 RLTGVELALRKELESCRVEVDSLRRENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQ 660

Query: 661 NQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYG 720
           NQG+VLLNESTQFCS+LLEFIKEK  Q  P +HR EHI+NGLD  FF+ESE KIQ  K+G
Sbjct: 661 NQGLVLLNESTQFCSKLLEFIKEKVGQFCPTEHRMEHIKNGLDGQFFIESEIKIQSLKHG 720

Query: 721 IESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEK 780
           IESLTMSLQKISMLLQA+SN TS +SGVD  LQLN QY EDGLRSELKAETLFSSLL+EK
Sbjct: 721 IESLTMSLQKISMLLQAKSNPTSQTSGVDT-LQLNCQYPEDGLRSELKAETLFSSLLREK 780

Query: 781 LFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKL 839
           L+SKELEVEQLQAEL TAVRGND+LKCEVQNGM+GLSCL+HK+KDLELQL  +NE+I+KL
Sbjct: 781 LYSKELEVEQLQAELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL 840

BLAST of Cp4.1LG01g04440 vs. TAIR 10
Match: AT3G55060.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39300.2); Has 61765 Blast hits to 33720 proteins in 2065 species: Archae - 846; Bacteria - 6964; Metazoa - 31967; Fungi - 5247; Plants - 3104; Viruses - 205; Other Eukaryotes - 13432 (source: NCBI BLink). )

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-152
Identity = 375/936 (40.06%), Postives = 535/936 (57.16%), Query Frame = 0

Query: 2   KKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLPSKF 61
           K FF RS GNG            +D +   E    S+M T    +A S  ++  D P K 
Sbjct: 3   KLFFFRSSGNG------------NDKQVNCEKEADSKMRT----QASSQAEQEFDSP-KS 62

Query: 62  DRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRS--TGNASSRSKRQHEQ 121
             Q+           LRR+ S SSA F       FD   + S++  T +A+    R+   
Sbjct: 63  HGQVSGG------LALRRSLSWSSAGFL------FDKFGETSKNELTTSATKSKDRRRNH 122

Query: 122 SSRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYID 181
           SSRC +P R+    V++ +   D          +  +DSSG+S++ SS VS++VLDRYID
Sbjct: 123 SSRCFTPERQ----VRERQCKAD----------KFQHDSSGSSSSCSSNVSSKVLDRYID 182

Query: 182 GEQH-----QEINGSKNKYSQRNNGWR-PPRAQCLPPSSTTASIKDNPRSYSSRETRSSL 241
           GE+H     Q+ N S +  S+  N  R PPR Q   P+S + +  +  +S S RE + + 
Sbjct: 183 GEEHLEPCKQKSNSSHSGVSESINRRRLPPRVQWTVPTSPSDTFDEKRKSQSFREAKGTH 242

Query: 242 SRFLSED-GEYGFGNDSPRS-AMTVVDRLSQQH--------------------------- 301
            R+ S D  + G  + SPRS A  V++RLSQ H                           
Sbjct: 243 LRYSSADCVDNGLRHGSPRSVARNVIERLSQTHGKSKGSNHEPITIQDVYGGSLNRTFDS 302

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 303 SSDIAANVSLAEHYEPVNEYYTQDYGGHQQNCIRSRNVYKCMEDDLDSELEMKIKEAEKR 362

Query: 362 ----------ERLVQYSKFDVSDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREEL 421
                     +R +    FDVS L+  I+ L  ER  LA E  +LL+S+I +R  AREE+
Sbjct: 363 AKLFSAELEQQRCLSDCDFDVSSLVGAIRKLEDERLHLAFENVNLLRSQIVERASAREEI 422

Query: 422 RQANEELESRTQKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQ 481
           R    + +   Q+LEKEK+ELQ GLEKELDRRS +W+ KLEK++LEE+  R RVRELAE 
Sbjct: 423 RWLKSDWDLHIQRLEKEKSELQAGLEKELDRRSGEWTSKLEKFQLEEKKLRERVRELAEH 482

Query: 482 NVSLQREVVSLNKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGS 541
           NVSLQRE+ + ++ ETENK M T+LE+ + +LT   D+ +E+N Y++  LSKL+E Y G+
Sbjct: 483 NVSLQRELSAFHENETENKDMITHLERRVAELTTTADKLHEENNYVKQTLSKLQESYAGA 542

Query: 542 IEGMDCIRKNFEEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLD 601
            E +D +R+NFEEK++ECRELHKS+T+  RTC EQ KTI GLR+ +SE+    QP EKLD
Sbjct: 543 TEDLDFLRRNFEEKDQECRELHKSVTKFFRTCKEQGKTIEGLRDGVSEEVKK-QPSEKLD 602

Query: 602 KEFEKLKMEQMRLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLV 661
           +  +KL++EQ+RLTG+EL+LR+ +ES ++E DSLR ENI +L  LK NG E   TT KL 
Sbjct: 603 QLVKKLQVEQVRLTGIELSLRREVESMKLETDSLRHENICLLNRLKGNGQEIDITTLKLE 662

Query: 662 NEMSTRVYHLQNQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLE 721
           NE+  RV +LQ QG+ +LNES+Q C +LL+FIK K  QL         +++GL   F +E
Sbjct: 663 NELKMRVCYLQEQGLSMLNESSQLCYKLLKFIKGKLTQLPETYQDKNSVKDGLSEQFMIE 722

Query: 722 SEAKIQGFKYGIESLTMSLQKISMLLQAESNSTSPSSGVDNALQLNSQYSEDGLRSELKA 781
           SE K+ G + G E+L  SLQ ++ ++   SNS S SS      +  +Q  E+ LR+EL A
Sbjct: 723 SEMKVHGIRRGTENLKRSLQTVTSVV--ASNSESSSSNTGRPREQRNQSVEENLRAELSA 782

Query: 782 ETLFSSLLKEKLFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQ 831
           ETL +SL++EKL+SKE E+EQLQAELA AVRGN++L+CEVQ+ ++ LS  +H++KDL+ Q
Sbjct: 783 ETLITSLVREKLYSKEKEIEQLQAELAAAVRGNEILRCEVQSSLDNLSVTTHELKDLKHQ 842

BLAST of Cp4.1LG01g04440 vs. TAIR 10
Match: AT2G39300.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: vacuole; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55060.1); Has 46416 Blast hits to 28308 proteins in 1743 species: Archae - 734; Bacteria - 4822; Metazoa - 24446; Fungi - 3539; Plants - 2267; Viruses - 163; Other Eukaryotes - 10445 (source: NCBI BLink). )

HSP 1 Score: 490.0 bits (1260), Expect = 3.9e-138
Identity = 361/923 (39.11%), Postives = 516/923 (55.90%), Query Frame = 0

Query: 1   MKK--FFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLP 60
           MKK  FF  S GNG   N  +H    D  + Y   P G                      
Sbjct: 1   MKKLFFFKSSSGNGTDHNKQLHKQKDDHFQRYLNSPKG---------------------- 60

Query: 61  SKFDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHE 120
                 ++ ++   S   LRR++SLSSAAF          +ID        SS   R   
Sbjct: 61  ------LNKSQSEVSGAALRRSRSLSSAAF----------VID------GTSSNQHRLRN 120

Query: 121 QSSRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYI 180
            SSRC +P R  QFK                          G+ +T SS VS++VLDRYI
Sbjct: 121 HSSRCLTPER--QFK------------------------EYGSMSTCSSNVSSQVLDRYI 180

Query: 181 DGEQHQEINGSK--NKYSQRNNGWR---PPRAQCLPPSSTTASIKDNPRSYSSRETRS-S 240
           DGE+H E +  K  + +S   +G R   PPRAQ   PS  + S KD  +S   R+  + S
Sbjct: 181 DGEEHLERSKQKSGSLHSSSLSGSRRRLPPRAQ--SPSPLSESGKDKRKSKGLRDASARS 240

Query: 241 LSRFLSEDGEYGFGNDSPRSA---------------------MTVVDRLSQQHERLVQY- 300
           L+R + E   +     S   +                       VV  L++++E + +Y 
Sbjct: 241 LARSVIERLSHNTQGKSKALSYEPIRIQDVCGKILDSNSDVLANVVVPLTEEYEPVNEYY 300

Query: 301 -------------------------------------------------------SKFDV 360
                                                                    FD+
Sbjct: 301 PDDQTELQYQQFFLHGKDMCKEDDVSSELEKRYKEAEKRVKLLSEEMEEKKFLSDCDFDI 360

Query: 361 SDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTEL 420
           S L+  I+ +  ER  LA EV SLL+S++ +R   RE++R+   + +   ++LEKEKTEL
Sbjct: 361 SSLVGDIRQMEEERVGLAFEVLSLLRSQMDERASTREDIRRVKNDWDLLLKRLEKEKTEL 420

Query: 421 QVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSM 480
           QV LE ELDRRSS+W+ K+E +K+EE+  R RVRELAE NVSLQRE+ + +++ETE   M
Sbjct: 421 QVQLETELDRRSSEWTSKVESFKVEEKRLRERVRELAEHNVSLQREISTFHEKETERIDM 480

Query: 481 TTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECREL 540
             +L++ + +L+A  +E  E+N +L  NLSKL+E Y GS + +D +R+NFEEK+ EC+EL
Sbjct: 481 IRHLDETVTELSATAEEMREENLFLMQNLSKLQESYTGSTDDLDYVRRNFEEKDMECKEL 540

Query: 541 HKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALR 600
           HKS+TRL RTC EQEKTI GLR+  SE+    QP E +DK   KL+MEQ+RL GVEL+LR
Sbjct: 541 HKSVTRLLRTCKEQEKTIQGLRDGFSEEIKK-QPSEHVDK---KLQMEQLRLVGVELSLR 600

Query: 601 KALESCRVEVDSLRRENINILTHLKDNGNERG-ATTFKLVNEMSTRVYHLQNQGMVLLNE 660
           K +ES ++E +SLRREN  +L  +K NG E    TTFKL NEM  RV HLQ+QG+ +LNE
Sbjct: 601 KEVESMKLEAESLRRENNCLLNRVKGNGEEADIMTTFKLDNEMKMRVCHLQDQGISMLNE 660

Query: 661 STQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQ 720
           STQ C + L+ IKEK+            + +G    F +ESE ++ G + G ESL  SLQ
Sbjct: 661 STQLCYKFLKIIKEKS------------VNSGWSEQFLIESEMRVHGIRRGTESLKRSLQ 720

Query: 721 KISMLLQAESN--STSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELE 780
            ++ LL  +SN  +++  S   +A + +S+  E  LR+EL+AETL +SLL+EKL+SKE E
Sbjct: 721 TVTSLLLEKSNEMASNSESSCSSAARPSSRSVEKSLRAELRAETLVTSLLREKLYSKEQE 780

Query: 781 VEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEES 835
           +EQL AE+A  VRGN++L+CE+QN ++ LS  +H++KDL+LQ++K++E+IN+++  L+E+
Sbjct: 781 IEQLHAEVAAGVRGNEVLQCEIQNVLDNLSLNNHQLKDLKLQMVKKDENINRMEINLQEA 831

BLAST of Cp4.1LG01g04440 vs. TAIR 10
Match: AT2G39300.2 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55060.1). )

HSP 1 Score: 490.0 bits (1260), Expect = 3.9e-138
Identity = 361/923 (39.11%), Postives = 516/923 (55.90%), Query Frame = 0

Query: 1   MKK--FFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTSIGDKAGSSPQRSKDLP 60
           MKK  FF  S GNG   N  +H    D  + Y   P G                      
Sbjct: 1   MKKLFFFKSSSGNGTDHNKQLHKQKDDHFQRYLNSPKG---------------------- 60

Query: 61  SKFDRQIDDNERSCSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSTGNASSRSKRQHE 120
                 ++ ++   S   LRR++SLSSAAF          +ID        SS   R   
Sbjct: 61  ------LNKSQSEVSGAALRRSRSLSSAAF----------VID------GTSSNQHRLRN 120

Query: 121 QSSRCQSPSREMQFKVKQTELPNDYTSGSVRPCSRTCYDSSGNSTTSSSIVSNRVLDRYI 180
            SSRC +P R  QFK                          G+ +T SS VS++VLDRYI
Sbjct: 121 HSSRCLTPER--QFK------------------------EYGSMSTCSSNVSSQVLDRYI 180

Query: 181 DGEQHQEINGSK--NKYSQRNNGWR---PPRAQCLPPSSTTASIKDNPRSYSSRETRS-S 240
           DGE+H E +  K  + +S   +G R   PPRAQ   PS  + S KD  +S   R+  + S
Sbjct: 181 DGEEHLERSKQKSGSLHSSSLSGSRRRLPPRAQ--SPSPLSESGKDKRKSKGLRDASARS 240

Query: 241 LSRFLSEDGEYGFGNDSPRSA---------------------MTVVDRLSQQHERLVQY- 300
           L+R + E   +     S   +                       VV  L++++E + +Y 
Sbjct: 241 LARSVIERLSHNTQGKSKALSYEPIRIQDVCGKILDSNSDVLANVVVPLTEEYEPVNEYY 300

Query: 301 -------------------------------------------------------SKFDV 360
                                                                    FD+
Sbjct: 301 PDDQTELQYQQFFLHGKDMCKEDDVSSELEKRYKEAEKRVKLLSEEMEEKKFLSDCDFDI 360

Query: 361 SDLIQIIKNLTGERFALALEVSSLLQSRIGDRTCAREELRQANEELESRTQKLEKEKTEL 420
           S L+  I+ +  ER  LA EV SLL+S++ +R   RE++R+   + +   ++LEKEKTEL
Sbjct: 361 SSLVGDIRQMEEERVGLAFEVLSLLRSQMDERASTREDIRRVKNDWDLLLKRLEKEKTEL 420

Query: 421 QVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVVSLNKRETENKSM 480
           QV LE ELDRRSS+W+ K+E +K+EE+  R RVRELAE NVSLQRE+ + +++ETE   M
Sbjct: 421 QVQLETELDRRSSEWTSKVESFKVEEKRLRERVRELAEHNVSLQREISTFHEKETERIDM 480

Query: 481 TTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNFEEKEKECREL 540
             +L++ + +L+A  +E  E+N +L  NLSKL+E Y GS + +D +R+NFEEK+ EC+EL
Sbjct: 481 IRHLDETVTELSATAEEMREENLFLMQNLSKLQESYTGSTDDLDYVRRNFEEKDMECKEL 540

Query: 541 HKSITRLTRTCNEQEKTINGLRERLSEQFGNIQPMEKLDKEFEKLKMEQMRLTGVELALR 600
           HKS+TRL RTC EQEKTI GLR+  SE+    QP E +DK   KL+MEQ+RL GVEL+LR
Sbjct: 541 HKSVTRLLRTCKEQEKTIQGLRDGFSEEIKK-QPSEHVDK---KLQMEQLRLVGVELSLR 600

Query: 601 KALESCRVEVDSLRRENINILTHLKDNGNERG-ATTFKLVNEMSTRVYHLQNQGMVLLNE 660
           K +ES ++E +SLRREN  +L  +K NG E    TTFKL NEM  RV HLQ+QG+ +LNE
Sbjct: 601 KEVESMKLEAESLRRENNCLLNRVKGNGEEADIMTTFKLDNEMKMRVCHLQDQGISMLNE 660

Query: 661 STQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYGIESLTMSLQ 720
           STQ C + L+ IKEK+            + +G    F +ESE ++ G + G ESL  SLQ
Sbjct: 661 STQLCYKFLKIIKEKS------------VNSGWSEQFLIESEMRVHGIRRGTESLKRSLQ 720

Query: 721 KISMLLQAESN--STSPSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLKEKLFSKELE 780
            ++ LL  +SN  +++  S   +A + +S+  E  LR+EL+AETL +SLL+EKL+SKE E
Sbjct: 721 TVTSLLLEKSNEMASNSESSCSSAARPSSRSVEKSLRAELRAETLVTSLLREKLYSKEQE 780

Query: 781 VEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKLQNELEES 835
           +EQL AE+A  VRGN++L+CE+QN ++ LS  +H++KDL+LQ++K++E+IN+++  L+E+
Sbjct: 781 IEQLHAEVAAGVRGNEVLQCEIQNVLDNLSLNNHQLKDLKLQMVKKDENINRMEINLQEA 831