Cp4.1LG01g04170 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g04170
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWAT1-related protein
LocationCp4.1LG01: 1462456 .. 1464733 (+)
RNA-Seq ExpressionCp4.1LG01g04170
SyntenyCp4.1LG01g04170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAAGGTTGCCTTTTCGTTTTCCATCGTTTATGAACTAATAAAAACTCCATTTTGGGAGCTTAAATAAGAGCCACCCACTTTGTGCGTTTCCCAATTTGAATGAGGGAGAGGGAGAGACAGCCATGGAGCTTAGGAGTGGAGCTTTGCTATGGGGAGTGGTTCCTTTTTTGGCGATGGTCACCTTGGAAGGCTGCACCATTGGACTAACTATTTTGGCTAAGACAGCCATTACTTATGGAACCAACACCTTTGTTTTTGTTGTCTACACAAACGCTGTTGCTTCCTTCCTTCTTCTTCCTTATTCCTTCCTTTTTCACTTCAACGACAGGTTCTAACTCCACTCCGACCAATTCCTCTTCCATCTTCTTCTTCTAATTTTCTTCATTAACCCTTTTTTTTTTGTTAATATTATGCAGAACGGATTATCAACAATCTTTGTTTTCCTTTCCTCTTCTCCTCCGAATCTTCCTCCTCGGCTTCACTGGGTTTGTCTTCTCGCTCCCTCTAATACATGAATAAACTAAGAACACGTCTAGATGAGAACGATTCTCGATAGATTCCTTCAGAAGGGTTAAAAAGAATTAAAAGTTACTATGCCTATAAGCAACAACGTGTACTTTTCGTTTCGGTGATTCAATTGTATCGTAAGAATTCTAAAGTTAAAAGCTTTTTTTTTTTTTTTCGAGTAATTCTACGTTGGGTGAAAAGTAACGTAAATTAACTGATCAAATCTAGATTTTGAATTTTGATCTGAATTCTAACGACGACCCATTTTAAGAAATCACTCGAGGTAGAATAATATAACTGATCAGGCCTTGTCATGTTACTGTGAGAATATAAAGTTTTGATTTTTATTTTTTATTATTATTGTGGTGTAGGATATGTGTCTCTCAGAACCTTGCGTTTTTGGGCCTAAGCTACAGCTCTCCAATTGTTGTATGCGTAATGGGTCTTATGCTTCCAGCCATTTCTTTCATTCTCTCAATCCTTCTCAGGTGCTCGTTTTATCTTTCTCGATCTCGATCTCGATTACGATCACGATCAGATAAACAAGAAGAAACATTTCCAGGAAAATGGGAGATTAGTAGTTCTTCTAACACTAGGTTTAAATTTCAGGAGAACAAAAATTAACTGGAAAAGTCCAAGTTTCATTACAAAAGTAGTTGGCACAGTTATATCAGTTATAGGAGCTACATCGGAGGAGCTTTACCTAGGCCCCGTTGTTCGTCAGCATCCATCTTCTTCTTTACATTTACAATTCAAACAAAAGCTCTTGGTCTTCACTTCAACCACAGATCGTTGGATCATGGGTGGCCTTTTATTAGCAGCTGCCACTCTATGTGTCTCAATATGGAACATCATTCAGGTTTATATCAATGTTTTATGACAAACCCATGATGTTTTTTTTGCATTTCTTGACACCCTTTTGTGTGTTTATGATTTTGGTCAGTTGGGTGTGGTGAAACAATACTCACAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCAGTTTAATGTCCGCAGCCATGGCTTTCTTTATTGTCGATGACTTGAATGCATGGATAATCAAATCAAAATTCGACATCTTTCTAATCATTGCAACGGTAAGAAAAACGAATTTGAATCATCACCTCGTATTATACATTGTTTCAAAGCGTTGAATACGATGAAATTTGCAGGGTGTTTTTAGCGGTTTGATTAGGAATCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCGTTTGGTATTCTTTTTGCTACTTTCTTTGGCTCCACCTTTTTTGGAGATACTTTCCACTACGGAAGGTAAAATTATTGTTTGTCTTTTGAATTAATCTCTTTTGGGTTTGTTGGATTGTGTTCAAGGAGTCGTTTTTTGTGGTAAAGTTTGAATTTTTTTGTGGTATGATGGCAGTGTGATGGCGGCGTTCATAGCTGGAATGGGATATTTGACTGTAATGTGGGGGCAAATGAATGAAGATCAAGGAAGAGATGATGAAAGCAACGATGATTTGTCTTCTTCTAGAGTTCCACTTCTGGATGAAGAATCAAAAGTGTAAAATACAATATCTAATATGATCAGAAGAAGTTATTTTGGAATGAATTCTGATCATATGAAGCTTTTTGTTTTCATGGAATCTCAATCTTTGGCGTTGAGAGGAATTGTATATATATTGTTCTAGTGTTGAGTGGGATCCTTTGTGTTTTTGTATAAATGAAATGATGATTGTTTTTTTTCTTATCTC

mRNA sequence

TGAAAGGTTGCCTTTTCGTTTTCCATCGTTTATGAACTAATAAAAACTCCATTTTGGGAGCTTAAATAAGAGCCACCCACTTTGTGCGTTTCCCAATTTGAATGAGGGAGAGGGAGAGACAGCCATGGAGCTTAGGAGTGGAGCTTTGCTATGGGGAGTGGTTCCTTTTTTGGCGATGGTCACCTTGGAAGGCTGCACCATTGGACTAACTATTTTGGCTAAGACAGCCATTACTTATGGAACCAACACCTTTGTTTTTGTTGTCTACACAAACGCTGTTGCTTCCTTCCTTCTTCTTCCTTATTCCTTCCTTTTTCACTTCAACGACAGGATATGTGTCTCTCAGAACCTTGCGTTTTTGGGCCTAAGCTACAGCTCTCCAATTGTTGTATGCGTAATGGGTCTTATGCTTCCAGCCATTTCTTTCATTCTCTCAATCCTTCTCAGGAGAACAAAAATTAACTGGAAAAGTCCAAGTTTCATTACAAAAGTAGTTGGCACAGTTATATCAGTTATAGGAGCTACATCGGAGGAGCTTTACCTAGGCCCCGTTGTTCGTCAGCATCCATCTTCTTCTTTACATTTACAATTCAAACAAAAGCTCTTGGTCTTCACTTCAACCACAGATCGTTGGATCATGGGTGGCCTTTTATTAGCAGCTGCCACTCTATGTGTCTCAATATGGAACATCATTCAGTTGGGTGTGGTGAAACAATACTCACAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCAGTTTAATGTCCGCAGCCATGGCTTTCTTTATTGTCGATGACTTGAATGCATGGATAATCAAATCAAAATTCGACATCTTTCTAATCATTGCAACGGGTGTTTTTAGCGGTTTGATTAGGAATCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCGTTTGGTATTCTTTTTGCTACTTTCTTTGGCTCCACCTTTTTTGGAGATACTTTCCACTACGGAAGTGTGATGGCGGCGTTCATAGCTGGAATGGGATATTTGACTGTAATGTGGGGGCAAATGAATGAAGATCAAGGAAGAGATGATGAAAGCAACGATGATTTGTCTTCTTCTAGAGTTCCACTTCTGGATGAAGAATCAAAAGTGTAAAATACAATATCTAATATGATCAGAAGAAGTTATTTTGGAATGAATTCTGATCATATGAAGCTTTTTGTTTTCATGGAATCTCAATCTTTGGCGTTGAGAGGAATTGTATATATATTGTTCTAGTGTTGAGTGGGATCCTTTGTGTTTTTGTATAAATGAAATGATGATTGTTTTTTTTCTTATCTC

Coding sequence (CDS)

ATGGAGCTTAGGAGTGGAGCTTTGCTATGGGGAGTGGTTCCTTTTTTGGCGATGGTCACCTTGGAAGGCTGCACCATTGGACTAACTATTTTGGCTAAGACAGCCATTACTTATGGAACCAACACCTTTGTTTTTGTTGTCTACACAAACGCTGTTGCTTCCTTCCTTCTTCTTCCTTATTCCTTCCTTTTTCACTTCAACGACAGGATATGTGTCTCTCAGAACCTTGCGTTTTTGGGCCTAAGCTACAGCTCTCCAATTGTTGTATGCGTAATGGGTCTTATGCTTCCAGCCATTTCTTTCATTCTCTCAATCCTTCTCAGGAGAACAAAAATTAACTGGAAAAGTCCAAGTTTCATTACAAAAGTAGTTGGCACAGTTATATCAGTTATAGGAGCTACATCGGAGGAGCTTTACCTAGGCCCCGTTGTTCGTCAGCATCCATCTTCTTCTTTACATTTACAATTCAAACAAAAGCTCTTGGTCTTCACTTCAACCACAGATCGTTGGATCATGGGTGGCCTTTTATTAGCAGCTGCCACTCTATGTGTCTCAATATGGAACATCATTCAGTTGGGTGTGGTGAAACAATACTCACAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCAGTTTAATGTCCGCAGCCATGGCTTTCTTTATTGTCGATGACTTGAATGCATGGATAATCAAATCAAAATTCGACATCTTTCTAATCATTGCAACGGGTGTTTTTAGCGGTTTGATTAGGAATCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCGTTTGGTATTCTTTTTGCTACTTTCTTTGGCTCCACCTTTTTTGGAGATACTTTCCACTACGGAAGTGTGATGGCGGCGTTCATAGCTGGAATGGGATATTTGACTGTAATGTGGGGGCAAATGAATGAAGATCAAGGAAGAGATGATGAAAGCAACGATGATTTGTCTTCTTCTAGAGTTCCACTTCTGGATGAAGAATCAAAAGTGTAA

Protein sequence

MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLDEESKV
Homology
BLAST of Cp4.1LG01g04170 vs. ExPASy Swiss-Prot
Match: F4I5D5 (WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 4.0e-86
Identity = 173/351 (49.29%), Postives = 237/351 (67.52%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDR-- 71
           +VPF+AM  +E CTI LTI+AKTA+T G + FVFVVYTNA  S LLLP+SFLFH N+R  
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 72  -------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILLRRT 131
                              I + QNLAF+GL +SSPIVVC MGL +P+ SF+LSI+L R+
Sbjct: 70  QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILGRS 129

Query: 132 KINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLH--LQFKQKLLVFTSTTD 191
           K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   KLLV+ +  D
Sbjct: 130 KLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNLPD 189

Query: 192 RWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLN 251
            W +G + LA A   VS++N++Q G VK+Y  VMKVASFYS+VG++     + F+  DL+
Sbjct: 190 NWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERDLS 249

Query: 252 AWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFG 311
           AW I+  FD++LIIATG F  +IR  V + C QMKGPYYVP+FKPFGI +AT FG++FF 
Sbjct: 250 AWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSFFV 309

Query: 312 DTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLDEE 340
           ++ HYGSV+ A IAG+GY TV WGQ+ E + +   + +  S   +   DE+
Sbjct: 310 NSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDED 360

BLAST of Cp4.1LG01g04170 vs. ExPASy Swiss-Prot
Match: Q9ZUI8 (WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-82
Identity = 176/355 (49.58%), Postives = 226/355 (63.66%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDR-- 71
           +VPF+ M  +E CTI LTILAKTA+T G + FVF+VYTNA+ S LLLPYSF FH ++   
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 72  -------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILL-RR 131
                              + + QN+AFLGLSYSSPIVVC MGL  PA SF+LS+ L + 
Sbjct: 71  EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALGKE 130

Query: 132 TKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL---LVFTST 191
             + W S     +V+GT+I   GA  E +YLGP +R  P SS    F   +   L F   
Sbjct: 131 GGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFFKN 190

Query: 192 TDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDD 251
           +D W +G LLLA ATL +SIWNIIQL  V++Y QVMKV S YSL G+L  A  + F+  D
Sbjct: 191 SDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFMEPD 250

Query: 252 LNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTF 311
           L+AW +K   D++LIIATG+F  +IR  VQ+ C +MKGPYYVP+FKPFGIL+A+ FG++F
Sbjct: 251 LSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGTSF 310

Query: 312 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDD-ESND----DLSSSRVPLL 337
           F ++ HYGSV+ A IAG GYL +MW Q+ +D   +  E ND    D      PLL
Sbjct: 311 FVNSLHYGSVLGAAIAGTGYLLIMWSQVQKDDPNETVEKNDNHQLDSDEQTTPLL 365

BLAST of Cp4.1LG01g04170 vs. ExPASy Swiss-Prot
Match: Q9FL08 (WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.7e-42
Identity = 106/324 (32.72%), Postives = 169/324 (52.16%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRIC 71
           VVPF AM  +E  T+G   L K A   G + +VFV Y+  V++ LLLP S +F  + R+ 
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  VSQNLAFL------------------GLSYSSPIVVCVMGLMLPAISFILSILLRRTKIN 131
            +++  F                   G++YSSP +   +  + PA +F L+++ R  ++ 
Sbjct: 78  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 137

Query: 132 WKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTSTTDRWIMG 191
            +S +   K++G ++S+ GA    LY GP V    S +  L         TS    WI+G
Sbjct: 138 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 197

Query: 192 GLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAWIIK 251
           GLLLA+    +S+W I+Q  V++ Y + + V  FY+L  +L+S  +  F   +L +W++K
Sbjct: 198 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 257

Query: 252 SKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDTFHY 311
               +  II +GVF  L       W + +KGP Y+ +F+P  I  A   G+ F GD  H 
Sbjct: 258 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 317

Query: 312 GSVMAAFIAGMGYLTVMWGQMNED 318
           GSV+ + I  +G+ TV+WG+  ED
Sbjct: 318 GSVIGSMILCIGFYTVIWGKARED 341

BLAST of Cp4.1LG01g04170 vs. ExPASy Swiss-Prot
Match: Q94JU2 (WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 118/360 (32.78%), Postives = 183/360 (50.83%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRI- 71
           V+P  A+V +E   +GL  L K A   G +  VF+VY+  +A+ LLLP S    F  R  
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLP-SLFCSFRSRTL 69

Query: 72  ------------------CVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILLRRTKI 131
                             C S  + + G++YSSP +   +  + PA +F+L+++ R   +
Sbjct: 70  PPMNFSILYKIVLLGIIGCCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFRMESV 129

Query: 132 NWKSPSFITKVVGTVISVIGATSEELYLGPVV--RQHPSSSLHLQFKQKLLVFTSTTDRW 191
           ++K  S + K++GTV+S+ GA    LY GPVV  +  PS SL  Q         ST   W
Sbjct: 130 SFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------STNPNW 189

Query: 192 IMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIV-DDLNA 251
           I+G   LA    CV +W I+Q  ++++Y     V  FYS+  S  +A +  F   +DL A
Sbjct: 190 ILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGNDLGA 249

Query: 252 WIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGD 311
           W IK    +  I+ +G+F   I N +  W +++KGP +V MFKP  I  A   G  F  D
Sbjct: 250 WKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVIFLRD 309

Query: 312 TFHYGSVMAAFIAGMGYLTVMWGQMNE-------DQGRDDESND-DL----SSSRVPLLD 338
           + + GS++ A +  +G+ TVMWG+  E       ++   +E+N+ DL     S + PLL+
Sbjct: 310 SLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKAPLLE 359

BLAST of Cp4.1LG01g04170 vs. ExPASy Swiss-Prot
Match: F4KHA8 (WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-40
Identity = 111/327 (33.94%), Postives = 172/327 (52.60%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRIC 71
           VVPF AMV +E  T+G   L K A   G + +VFV YT  VA+ +LLP S +F  + R+ 
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATLVLLPLSLIFGRSKRLP 78

Query: 72  VSQNLAFL------------------GLSYSSPIVVCVMGLMLPAISFILSILLRRTKIN 131
            ++   F                   G+ YSSP +   +  + PA +F L+++ R  +I 
Sbjct: 79  SAKTPVFFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLTPAFTFTLAVIFRMEQIV 138

Query: 132 WKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPS---SSLHLQFKQKLLVFTSTTDRW 191
            +S +   K++GT++S+ GA    LY GP V    S    S  +   Q L  F S+   W
Sbjct: 139 LRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQHLTSFDSS---W 198

Query: 192 IMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAW 251
           I+GGLLLA   L VS+W I+Q  V++ Y + + V   Y+L  +L+SA +  F   DLN++
Sbjct: 199 IIGGLLLATQYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSF 258

Query: 252 IIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDT 311
           I+K    +  ++ +G       + +  W + +KGP Y+ +FKP  I+ A   G  F GD 
Sbjct: 259 ILKPGVSLASVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLGDA 318

Query: 312 FHYGSVMAAFIAGMGYLTVMWGQMNED 318
            + GSV+ + I  +G+ TV+WG+  ED
Sbjct: 319 LYLGSVIGSLILSLGFYTVIWGKARED 342

BLAST of Cp4.1LG01g04170 vs. NCBI nr
Match: XP_023529184.1 (WAT1-related protein At1g70260-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 636 bits (1641), Expect = 6.24e-229
Identity = 342/365 (93.70%), Postives = 342/365 (93.70%), Query Frame = 0

Query: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60
           MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY
Sbjct: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60

Query: 61  SFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLP 120
           SFLFHFNDR                       ICVSQNLAFLGLSYSSPIVVCVMGLMLP
Sbjct: 61  SFLFHFNDRTDYQQSLFSFPLLLRIFLLGFTGICVSQNLAFLGLSYSSPIVVCVMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK
Sbjct: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180

Query: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240
           QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA
Sbjct: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240

Query: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
           AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL
Sbjct: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300

Query: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 342
           FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD
Sbjct: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 360

BLAST of Cp4.1LG01g04170 vs. NCBI nr
Match: XP_022941741.1 (WAT1-related protein At1g70260-like [Cucurbita moschata])

HSP 1 Score: 632 bits (1631), Expect = 2.08e-227
Identity = 340/365 (93.15%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60
           MELRSGALLWGVVPFLAMV+LEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY
Sbjct: 1   MELRSGALLWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60

Query: 61  SFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLP 120
           SFLFH NDR                       ICVSQNLAFLGLSYSSPIVVCVMGLMLP
Sbjct: 61  SFLFHLNDRTDYQQSLFSLPLLLRIFLLGFIGICVSQNLAFLGLSYSSPIVVCVMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK
Sbjct: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180

Query: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240
           QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA
Sbjct: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240

Query: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
           AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL
Sbjct: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300

Query: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 342
           FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD
Sbjct: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 360

BLAST of Cp4.1LG01g04170 vs. NCBI nr
Match: KAG6600128.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630 bits (1624), Expect = 2.43e-226
Identity = 338/365 (92.60%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60
           MELRSGALLWGVVPFLAMV+LEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY
Sbjct: 1   MELRSGALLWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60

Query: 61  SFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLP 120
           SFLFH NDR                       ICVSQNLAFLGLSYSSPIVVCVMGLMLP
Sbjct: 61  SFLFHLNDRTDYQQSLFSLPLLLRIFLLGFIGICVSQNLAFLGLSYSSPIVVCVMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK
Sbjct: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180

Query: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240
           QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA
Sbjct: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240

Query: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
           AM+FFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL
Sbjct: 241 AMSFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300

Query: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 342
           FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDD+SNDDLSSSRVPLLD
Sbjct: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDKSNDDLSSSRVPLLD 360

BLAST of Cp4.1LG01g04170 vs. NCBI nr
Match: XP_022985587.1 (WAT1-related protein At1g70260-like [Cucurbita maxima])

HSP 1 Score: 615 bits (1585), Expect = 2.20e-220
Identity = 333/366 (90.98%), Postives = 337/366 (92.08%), Query Frame = 0

Query: 1   MELRSGALLWGVV-PFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP 60
           MELRSGALLWGVV PFLAMV+LEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP
Sbjct: 1   MELRSGALLWGVVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP 60

Query: 61  YSFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLML 120
           YSFLFH N R                       ICVSQNLAFLGLSYSSPIVVCVMGLML
Sbjct: 61  YSFLFHLNHRTDYQQSLFSFPLLLRIFLLGFTGICVSQNLAFLGLSYSSPIVVCVMGLML 120

Query: 121 PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQF 180
           PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHL F
Sbjct: 121 PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLHF 180

Query: 181 KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMS 240
           KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQ MKVASFYSLVG+LMS
Sbjct: 181 KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQGMKVASFYSLVGTLMS 240

Query: 241 AAMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI 300
           AAMAFF VDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI
Sbjct: 241 AAMAFFFVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI 300

Query: 301 LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLL 342
           LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDQGRDD+SNDDLSSSRVPLL
Sbjct: 301 LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDQGRDDKSNDDLSSSRVPLL 360

BLAST of Cp4.1LG01g04170 vs. NCBI nr
Match: XP_022957168.1 (WAT1-related protein At1g70260-like [Cucurbita moschata])

HSP 1 Score: 547 bits (1410), Expect = 1.02e-193
Identity = 288/361 (79.78%), Postives = 316/361 (87.53%), Query Frame = 0

Query: 9   LWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFND 68
           +WGVVPFLAMV+LEGCTI LTI+AKTAITYG  TFVFVVYTNAVAS +LLPYSF+FH+N+
Sbjct: 7   IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSFIFHYNE 66

Query: 69  R-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSI 128
           R                       ICVSQNLAFLGLSYSSPIVVC MGLMLPAISF+LSI
Sbjct: 67  RTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPAISFVLSI 126

Query: 129 LLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTS 188
           LL RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK KLLVFTS
Sbjct: 127 LLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTS 186

Query: 189 TTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVD 248
           TTDRWIMGGLLLAAA+LCVSIWNI+QLGV+KQY QVMKVASFYSLVG+L+SAA+ FF+V+
Sbjct: 187 TTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVN 246

Query: 249 DLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGST 308
           D +AW IKS FD+FLIIATG+FS LIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFG T
Sbjct: 247 DSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVT 306

Query: 309 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQG----RDDESNDDLSSSRVPLLDEESK 342
           FFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED G    +DD++ND LSS+++PLLDEESK
Sbjct: 307 FFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDKNNDHLSSAKLPLLDEESK 366

BLAST of Cp4.1LG01g04170 vs. ExPASy TrEMBL
Match: A0A6J1FLY1 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447017 PE=3 SV=1)

HSP 1 Score: 632 bits (1631), Expect = 1.01e-227
Identity = 340/365 (93.15%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60
           MELRSGALLWGVVPFLAMV+LEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY
Sbjct: 1   MELRSGALLWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60

Query: 61  SFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLP 120
           SFLFH NDR                       ICVSQNLAFLGLSYSSPIVVCVMGLMLP
Sbjct: 61  SFLFHLNDRTDYQQSLFSLPLLLRIFLLGFIGICVSQNLAFLGLSYSSPIVVCVMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK
Sbjct: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180

Query: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240
           QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA
Sbjct: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240

Query: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
           AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL
Sbjct: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300

Query: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 342
           FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD
Sbjct: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLD 360

BLAST of Cp4.1LG01g04170 vs. ExPASy TrEMBL
Match: A0A6J1J595 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111483609 PE=3 SV=1)

HSP 1 Score: 615 bits (1585), Expect = 1.07e-220
Identity = 333/366 (90.98%), Postives = 337/366 (92.08%), Query Frame = 0

Query: 1   MELRSGALLWGVV-PFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP 60
           MELRSGALLWGVV PFLAMV+LEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP
Sbjct: 1   MELRSGALLWGVVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLP 60

Query: 61  YSFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLML 120
           YSFLFH N R                       ICVSQNLAFLGLSYSSPIVVCVMGLML
Sbjct: 61  YSFLFHLNHRTDYQQSLFSFPLLLRIFLLGFTGICVSQNLAFLGLSYSSPIVVCVMGLML 120

Query: 121 PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQF 180
           PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHL F
Sbjct: 121 PAISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLHF 180

Query: 181 KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMS 240
           KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQ MKVASFYSLVG+LMS
Sbjct: 181 KQKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQGMKVASFYSLVGTLMS 240

Query: 241 AAMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI 300
           AAMAFF VDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI
Sbjct: 241 AAMAFFFVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGI 300

Query: 301 LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLL 342
           LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDQGRDD+SNDDLSSSRVPLL
Sbjct: 301 LFATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDQGRDDKSNDDLSSSRVPLL 360

BLAST of Cp4.1LG01g04170 vs. ExPASy TrEMBL
Match: A0A6J1GZG2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 4.95e-194
Identity = 288/361 (79.78%), Postives = 316/361 (87.53%), Query Frame = 0

Query: 9   LWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFND 68
           +WGVVPFLAMV+LEGCTI LTI+AKTAITYG  TFVFVVYTNAVAS +LLPYSF+FH+N+
Sbjct: 7   IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSFIFHYNE 66

Query: 69  R-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSI 128
           R                       ICVSQNLAFLGLSYSSPIVVC MGLMLPAISF+LSI
Sbjct: 67  RTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPAISFVLSI 126

Query: 129 LLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTS 188
           LL RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK KLLVFTS
Sbjct: 127 LLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTS 186

Query: 189 TTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVD 248
           TTDRWIMGGLLLAAA+LCVSIWNI+QLGV+KQY QVMKVASFYSLVG+L+SAA+ FF+V+
Sbjct: 187 TTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVN 246

Query: 249 DLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGST 308
           D +AW IKS FD+FLIIATG+FS LIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFG T
Sbjct: 247 DSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVT 306

Query: 309 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQG----RDDESNDDLSSSRVPLLDEESK 342
           FFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED G    +DD++ND LSS+++PLLDEESK
Sbjct: 307 FFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDKNNDHLSSAKLPLLDEESK 366

BLAST of Cp4.1LG01g04170 vs. ExPASy TrEMBL
Match: A0A6J1IKD8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1)

HSP 1 Score: 543 bits (1398), Expect = 3.32e-192
Identity = 286/361 (79.22%), Postives = 314/361 (86.98%), Query Frame = 0

Query: 9   LWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFND 68
           +WGVVPFLAMV+LEGCTI LTI+AKTAITYG  TFVFVVYTNAVAS +LLPYS +FH+N+
Sbjct: 7   IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSLIFHYNE 66

Query: 69  R-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSI 128
           R                       ICVSQNLAFLGLSYSSPIVVC MGLMLPAISF+LSI
Sbjct: 67  RTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPAISFVLSI 126

Query: 129 LLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTS 188
           +L RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK KLLVFTS
Sbjct: 127 ILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTS 186

Query: 189 TTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVD 248
           TTDRWIMGGLLLAAA+LCVSIWNIIQLGV+KQY QVMKVASFYSLVG+L+SAA+ FF+V+
Sbjct: 187 TTDRWIMGGLLLAAASLCVSIWNIIQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVN 246

Query: 249 DLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGST 308
           D +AW IKS FD+FLIIATG+FS LIRNRVQIWCMQMKGPYYVPMFKPFGILFAT FG T
Sbjct: 247 DSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATLFGVT 306

Query: 309 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQG----RDDESNDDLSSSRVPLLDEESK 342
           FFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED G    +DD++ND LSS+++PLLDEESK
Sbjct: 307 FFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDKNNDHLSSAKLPLLDEESK 366

BLAST of Cp4.1LG01g04170 vs. ExPASy TrEMBL
Match: A0A1S3BEK3 (WAT1-related protein At1g70260 OS=Cucumis melo OX=3656 GN=LOC103489028 PE=4 SV=1)

HSP 1 Score: 527 bits (1357), Expect = 7.71e-186
Identity = 282/369 (76.42%), Postives = 315/369 (85.37%), Query Frame = 0

Query: 1   MELRSGALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPY 60
           ME++ G +L  VVPFLAMVT+EGCTI LTILAKTAITYG +TFVFVVYTNAVAS +LLPY
Sbjct: 3   MEVKGGRILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPY 62

Query: 61  SFLFHFNDR-----------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLP 120
           SF+FH+N R                       IC+SQNLAFLGLSYSSPIVVC MGLMLP
Sbjct: 63  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 122

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           +ISF+LSILL +TKI W++P+FITKVVGTVISVIGAT EELYLGP VRQHPSSS HLQFK
Sbjct: 123 SISFLLSILLGKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHPSSSTHLQFK 182

Query: 181 QKLLVFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSA 240
           QKLLVFTSTTDRWI GGLLLAAATLCVSIWNIIQLGVVKQYSQVMKV SFYS+VG+ MSA
Sbjct: 183 QKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMSA 242

Query: 241 AMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
            +A+F+V+D +AW IKS FD++LIIATG FSGLIRNRVQIWCMQ KGP+YVPMFKPFGIL
Sbjct: 243 IVAYFVVNDSSAWTIKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGIL 302

Query: 301 FATFFGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGR------DDESNDD-LSS 339
           FATFFG+TFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED+G       DD++NDD LSS
Sbjct: 303 FATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQVNEDRGVGKDKDVDDKNNDDSLSS 362

BLAST of Cp4.1LG01g04170 vs. TAIR 10
Match: AT1G70260.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 319.7 bits (818), Expect = 2.9e-87
Identity = 173/351 (49.29%), Postives = 237/351 (67.52%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDR-- 71
           +VPF+AM  +E CTI LTI+AKTA+T G + FVFVVYTNA  S LLLP+SFLFH N+R  
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 72  -------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILLRRT 131
                              I + QNLAF+GL +SSPIVVC MGL +P+ SF+LSI+L R+
Sbjct: 70  QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILGRS 129

Query: 132 KINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLH--LQFKQKLLVFTSTTD 191
           K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   KLLV+ +  D
Sbjct: 130 KLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNLPD 189

Query: 192 RWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLN 251
            W +G + LA A   VS++N++Q G VK+Y  VMKVASFYS+VG++     + F+  DL+
Sbjct: 190 NWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERDLS 249

Query: 252 AWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFG 311
           AW I+  FD++LIIATG F  +IR  V + C QMKGPYYVP+FKPFGI +AT FG++FF 
Sbjct: 250 AWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSFFV 309

Query: 312 DTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESNDDLSSSRVPLLDEE 340
           ++ HYGSV+ A IAG+GY TV WGQ+ E + +   + +  S   +   DE+
Sbjct: 310 NSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDED 360

BLAST of Cp4.1LG01g04170 vs. TAIR 10
Match: AT1G60050.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 176/355 (49.58%), Postives = 226/355 (63.66%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDR-- 71
           +VPF+ M  +E CTI LTILAKTA+T G + FVF+VYTNA+ S LLLPYSF FH ++   
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 72  -------------------ICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILL-RR 131
                              + + QN+AFLGLSYSSPIVVC MGL  PA SF+LS+ L + 
Sbjct: 71  EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALGKE 130

Query: 132 TKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL---LVFTST 191
             + W S     +V+GT+I   GA  E +YLGP +R  P SS    F   +   L F   
Sbjct: 131 GGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFFKN 190

Query: 192 TDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDD 251
           +D W +G LLLA ATL +SIWNIIQL  V++Y QVMKV S YSL G+L  A  + F+  D
Sbjct: 191 SDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFMEPD 250

Query: 252 LNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTF 311
           L+AW +K   D++LIIATG+F  +IR  VQ+ C +MKGPYYVP+FKPFGIL+A+ FG++F
Sbjct: 251 LSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGTSF 310

Query: 312 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDD-ESND----DLSSSRVPLL 337
           F ++ HYGSV+ A IAG GYL +MW Q+ +D   +  E ND    D      PLL
Sbjct: 311 FVNSLHYGSVLGAAIAGTGYLLIMWSQVQKDDPNETVEKNDNHQLDSDEQTTPLL 365

BLAST of Cp4.1LG01g04170 vs. TAIR 10
Match: AT5G40240.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-43
Identity = 106/324 (32.72%), Postives = 169/324 (52.16%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRIC 71
           VVPF AM  +E  T+G   L K A   G + +VFV Y+  V++ LLLP S +F  + R+ 
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  VSQNLAFL------------------GLSYSSPIVVCVMGLMLPAISFILSILLRRTKIN 131
            +++  F                   G++YSSP +   +  + PA +F L+++ R  ++ 
Sbjct: 78  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 137

Query: 132 WKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTSTTDRWIMG 191
            +S +   K++G ++S+ GA    LY GP V    S +  L         TS    WI+G
Sbjct: 138 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 197

Query: 192 GLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAWIIK 251
           GLLLA+    +S+W I+Q  V++ Y + + V  FY+L  +L+S  +  F   +L +W++K
Sbjct: 198 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 257

Query: 252 SKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDTFHY 311
               +  II +GVF  L       W + +KGP Y+ +F+P  I  A   G+ F GD  H 
Sbjct: 258 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 317

Query: 312 GSVMAAFIAGMGYLTVMWGQMNED 318
           GSV+ + I  +G+ TV+WG+  ED
Sbjct: 318 GSVIGSMILCIGFYTVIWGKARED 341

BLAST of Cp4.1LG01g04170 vs. TAIR 10
Match: AT5G40240.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-43
Identity = 106/324 (32.72%), Postives = 169/324 (52.16%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRIC 71
           VVPF AM  +E  T+G   L K A   G + +VFV Y+  V++ LLLP S +F  + R+ 
Sbjct: 32  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 91

Query: 72  VSQNLAFL------------------GLSYSSPIVVCVMGLMLPAISFILSILLRRTKIN 131
            +++  F                   G++YSSP +   +  + PA +F L+++ R  ++ 
Sbjct: 92  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 151

Query: 132 WKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLVFTSTTDRWIMG 191
            +S +   K++G ++S+ GA    LY GP V    S +  L         TS    WI+G
Sbjct: 152 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 211

Query: 192 GLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAWIIK 251
           GLLLA+    +S+W I+Q  V++ Y + + V  FY+L  +L+S  +  F   +L +W++K
Sbjct: 212 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 271

Query: 252 SKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDTFHY 311
               +  II +GVF  L       W + +KGP Y+ +F+P  I  A   G+ F GD  H 
Sbjct: 272 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 331

Query: 312 GSVMAAFIAGMGYLTVMWGQMNED 318
           GSV+ + I  +G+ TV+WG+  ED
Sbjct: 332 GSVIGSMILCIGFYTVIWGKARED 355

BLAST of Cp4.1LG01g04170 vs. TAIR 10
Match: AT3G28050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-42
Identity = 118/360 (32.78%), Postives = 183/360 (50.83%), Query Frame = 0

Query: 12  VVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHFNDRI- 71
           V+P  A+V +E   +GL  L K A   G +  VF+VY+  +A+ LLLP S    F  R  
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLP-SLFCSFRSRTL 69

Query: 72  ------------------CVSQNLAFLGLSYSSPIVVCVMGLMLPAISFILSILLRRTKI 131
                             C S  + + G++YSSP +   +  + PA +F+L+++ R   +
Sbjct: 70  PPMNFSILYKIVLLGIIGCCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFRMESV 129

Query: 132 NWKSPSFITKVVGTVISVIGATSEELYLGPVV--RQHPSSSLHLQFKQKLLVFTSTTDRW 191
           ++K  S + K++GTV+S+ GA    LY GPVV  +  PS SL  Q         ST   W
Sbjct: 130 SFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------STNPNW 189

Query: 192 IMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFFIV-DDLNA 251
           I+G   LA    CV +W I+Q  ++++Y     V  FYS+  S  +A +  F   +DL A
Sbjct: 190 ILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGNDLGA 249

Query: 252 WIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGD 311
           W IK    +  I+ +G+F   I N +  W +++KGP +V MFKP  I  A   G  F  D
Sbjct: 250 WKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVIFLRD 309

Query: 312 TFHYGSVMAAFIAGMGYLTVMWGQMNE-------DQGRDDESND-DL----SSSRVPLLD 338
           + + GS++ A +  +G+ TVMWG+  E       ++   +E+N+ DL     S + PLL+
Sbjct: 310 SLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKAPLLE 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I5D54.0e-8649.29WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2... [more]
Q9ZUI82.7e-8249.58WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3... [more]
Q9FL082.7e-4232.72WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2... [more]
Q94JU22.3e-4132.78WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2... [more]
F4KHA81.1e-4033.94WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3... [more]
Match NameE-valueIdentityDescription
XP_023529184.16.24e-22993.70WAT1-related protein At1g70260-like [Cucurbita pepo subsp. pepo][more]
XP_022941741.12.08e-22793.15WAT1-related protein At1g70260-like [Cucurbita moschata][more]
KAG6600128.12.43e-22692.60WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022985587.12.20e-22090.98WAT1-related protein At1g70260-like [Cucurbita maxima][more]
XP_022957168.11.02e-19379.78WAT1-related protein At1g70260-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1FLY11.01e-22793.15WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447017 PE=3 SV=1[more]
A0A6J1J5951.07e-22090.98WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111483609 PE=3 SV=1[more]
A0A6J1GZG24.95e-19479.78WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1[more]
A0A6J1IKD83.32e-19279.22WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1[more]
A0A1S3BEK37.71e-18676.42WAT1-related protein At1g70260 OS=Cucumis melo OX=3656 GN=LOC103489028 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70260.12.9e-8749.29nodulin MtN21 /EamA-like transporter family protein [more]
AT1G60050.11.9e-8349.58Nodulin MtN21 /EamA-like transporter family protein [more]
AT5G40240.11.9e-4332.72nodulin MtN21 /EamA-like transporter family protein [more]
AT5G40240.21.9e-4332.72nodulin MtN21 /EamA-like transporter family protein [more]
AT3G28050.11.6e-4232.78nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 318..342
NoneNo IPR availablePANTHERPTHR31218:SF61WAT1-RELATED PROTEINcoord: 10..339
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 10..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g04170.1Cp4.1LG01g04170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity