Cp4.1LG01g03300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g03300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPentatricopeptide repeat-containing protein
LocationCp4.1LG01: 1926715 .. 1928629 (+)
RNA-Seq ExpressionCp4.1LG01g03300
SyntenyCp4.1LG01g03300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGATGTGGACGGGCAGGATTGTGATTAAGCCCTAACAAAACCTCCGACGTATCACGCACCCAGGACAAGTGATTATCAAGAGAAATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAAACGCCGAGTCGTCGCGCGGTCCTGTGAATGATCAGCAGCAGCGGCTCTACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATCATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGGTTTTTTCCTTAAACACATAGGTTCGTTCACTGTAGGAGAAATATAAATGCACAAAATTACTGCTGCTTTTCATTTGTTTTTCAAATTCTATTGCCAATTGTTGATTGTATTCTTCCTTTCGCTGCTAGATCATAAATTTTTTGTAAACGAAACATTCATGAAATATAAAGGAAATGACAAAAACCTTATGAAGAATTTACGATAAAAAATCATTGGATCTGGGGTAGCTATAAAAAAGCAGTCTGGAGGGAGAACCAGCCAAAAAGTTGCTGTAATTGTAGGAGACCATAAGATCTTAGCTCATCCATTGCATTTGCTTCGGTTGTTGCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGTCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCGGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCCGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTTTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCCATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCTGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGAAAGAAGACAACATTGAGGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGA

mRNA sequence

CACGATGTGGACGGGCAGGATTGTGATTAAGCCCTAACAAAACCTCCGACGTATCACGCACCCAGGACAAGTGATTATCAAGAGAAATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAAACGCCGAGTCGTCGCGCGGTCCTGTGAATGATCAGCAGCAGCGGCTCTACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATCATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGTCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCGGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCCGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTTTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCCATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCTGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGAAAGAAGACAACATTGAGGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGA

Coding sequence (CDS)

ATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAAACGCCGAGTCGTCGCGCGGTCCTGTGAATGATCAGCAGCAGCGGCTCTACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATCATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGTCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCGGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCCGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTTTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCCATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCTGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGAAAGAAGACAACATTGAGGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGA

Protein sequence

MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELLSRQH
Homology
BLAST of Cp4.1LG01g03300 vs. ExPASy Swiss-Prot
Match: Q9FZ24 (Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g02370 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.2e-136
Identity = 248/470 (52.77%), Postives = 339/470 (72.13%), Query Frame = 0

Query: 29  VNDQQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQI 88
           V  +Q+ LY +LS L  TGG+VA+TLNQ+IMEG  V+K +L RC K LRK+RR  HA +I
Sbjct: 66  VASRQRELYKKLSMLSVTGGTVAETLNQFIMEGITVRKDDLFRCAKTLRKFRRPQHAFEI 125

Query: 89  MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKN-RFTYGALLNCYCKE 148
            +WME RK+ +S +D+A+ LDLI K KG+ AAENYF +L  SAKN + TYGAL+NCYC E
Sbjct: 126 FDWMEKRKMTFSVSDHAICLDLIGKTKGLEAAENYFNNLDPSAKNHQSTYGALMNCYCVE 185

Query: 149 LMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTY 208
           L EEKA A  + +DEL F +N L FNN+M+MYMR+ QPEKVP L+D MK+RGI     TY
Sbjct: 186 LEEEKAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPEKVPVLVDAMKQRGISPCGVTY 245

Query: 209 NVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVE 268
           ++WM SC SLN +  +E+I++EM  +   K  W TFSNLAAIY KAG  EKA+ ALK +E
Sbjct: 246 SIWMQSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNLAAIYTKAGLYEKADSALKSME 305

Query: 269 NEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLK 328
            ++  N   +R ++HFL+SLYA  S   EVYR+W +LK   P  NN+SYLVMLQA+SKL 
Sbjct: 306 EKMNPN---NRDSHHFLMSLYAGISKGPEVYRVWESLKKARPEVNNLSYLVMLQAMSKLG 365

Query: 329 DFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRARE 388
           D +G+K  + EWES C ++D+RLA++ I  YL+ +MY++A  + + A+K+SKGPF +AR+
Sbjct: 366 DLDGIKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEKILDGAMKKSKGPFSKARQ 425

Query: 389 MFMIYFLKFKQVDLALSHLESAISESMD--DEWHPSPAMANAFLMYFEEEKDVEGAEDFA 448
           + MI+ L+  + DLA+ HLE+A+S+S +  DEW  S  + + F ++FE+ KDV+GAEDF 
Sbjct: 426 LLMIHLLENDKADLAMKHLEAAVSDSAENKDEWGWSSELVSLFFLHFEKAKDVDGAEDFC 485

Query: 449 RILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELL 495
           +IL  +K LD+     L+KTYAAA K +PDMR+RL +  IEVS E+++LL
Sbjct: 486 KILSNWKPLDSETMTFLIKTYAAAEKTSPDMRERLSQQQIEVSEEIQDLL 532

BLAST of Cp4.1LG01g03300 vs. ExPASy Swiss-Prot
Match: Q93WC5 (Pentatricopeptide repeat-containing protein At4g01990, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g01990 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 3.3e-121
Identity = 229/467 (49.04%), Postives = 318/467 (68.09%), Query Frame = 0

Query: 32  QQQRLYPRLSKLGAT-GGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIME 91
           + + +Y +LS LG   GG + +TLNQ++MEG  VKK++L R  K+LRK+R+   AL+I E
Sbjct: 37  KHRSIYKKLSSLGTRGGGKMEETLNQFVMEGVPVKKHDLIRYAKDLRKFRQPQRALEIFE 96

Query: 92  WMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELME 151
           WME ++I ++ +D+A+RL+LI+K KG+ AAE YF  L  S KN+ TYG+LLNCYC E  E
Sbjct: 97  WMERKEIAFTGSDHAIRLNLIAKSKGLEAAETYFNSLDDSIKNQSTYGSLLNCYCVEKEE 156

Query: 152 EKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNVW 211
            KA A  + + +L   SN L FNNLM MYM + QPEKVP L+  MK + I     TY++W
Sbjct: 157 VKAKAHFENMVDLNHVSNSLPFNNLMAMYMGLGQPEKVPALVVAMKEKSITPCDITYSMW 216

Query: 212 MNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEI 271
           + SC SL  +  VE++L+EMK E    F W TF+NLAAIY+K G   KAE ALK +EN +
Sbjct: 217 IQSCGSLKDLDGVEKVLDEMKAEGEGIFSWNTFANLAAIYIKVGLYGKAEEALKSLENNM 276

Query: 272 KSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFE 331
             +    R  YHFLI+LY   +N SEVYR+W+ LK  YP  NN SYL ML+ALSKL D +
Sbjct: 277 NPDV---RDCYHFLINLYTGIANASEVYRVWDLLKKRYPNVNNSSYLTMLRALSKLDDID 336

Query: 332 GLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFM 391
           G+K  + EWES+C ++D+R+A+V I +YL+Q+MY++A  VF  A+K+ KG F +AR++ M
Sbjct: 337 GVKKVFAEWESTCWTYDMRMANVAISSYLKQNMYEEAEAVFNGAMKKCKGQFSKARQLLM 396

Query: 392 IYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKR 451
           ++ LK  Q DLAL H E+A+ +  D  W  S  + ++F ++FEE KDV+GAE+F + L +
Sbjct: 397 MHLLKNDQADLALKHFEAAVLD-QDKNWTWSSELISSFFLHFEEAKDVDGAEEFCKTLTK 456

Query: 452 FKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELLSR 497
           +  L +  Y LL+KTY AAGK  PDM++RL+E  I V  E E LLS+
Sbjct: 457 WSPLSSETYTLLMKTYLAAGKACPDMKKRLEEQGILVDEEQECLLSK 499

BLAST of Cp4.1LG01g03300 vs. ExPASy Swiss-Prot
Match: O22714 (Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX=3702 GN=At1g60770 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.9e-101
Identity = 206/485 (42.47%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 14  LRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCI 73
           +RHL  S   + R      ++ LY RL K G T   V Q LNQ++   K V K+E+   I
Sbjct: 3   MRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFKWEVGDTI 62

Query: 74  KELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKN 133
           K+LR    Y+ AL++ E ME R +N + +D A+ LDL++K + I A ENYF DL  ++K 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERGMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSKT 122

Query: 134 RFTYGALLNCYCKELMEEKALALSKKIDELKFA-SNLSFNNLMTMYMRMDQPEKVPPLID 193
             TYG+LLNCYCKEL+ EKA  L  K+ EL    S++S+N+LMT+Y +  + EKVP +I 
Sbjct: 123 ELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMIQ 182

Query: 194 EMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKA 253
           E+K   +   +YTYNVWM + A+ N +  VE ++EEM  + R   DWTT+SN+A+IYV A
Sbjct: 183 ELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVDA 242

Query: 254 GQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNN 313
           G  +KAE AL+++E     N Q+D  AY FLI+LY      +EVYRIW +L+   P T+N
Sbjct: 243 GLSQKAEKALQELE---MKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSN 302

Query: 314 MSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFED 373
           ++YL M+Q L KL D  G ++ +KEW+++CS++D+R+ +V IGAY ++ + + A  + E 
Sbjct: 303 VAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEK 362

Query: 374 AIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDD--EWHPSPAMANAFLMY 433
           A +R      +  E+FM Y++K   +  AL  +  A+S    D  +W PSP    A + Y
Sbjct: 363 APRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMSY 422

Query: 434 FEEEKDVEGAEDFARILKR-FKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSE 493
           FE++KDV GAE+   ILK     + A  +  L++TYAAAGK  P MR+RLK +N+EV+  
Sbjct: 423 FEQKKDVNGAENLLEILKNGTDNIGAEIFEPLIRTYAAAGKSHPAMRRRLKMENVEVNEA 482

Query: 494 LEELL 495
            ++LL
Sbjct: 483 TKKLL 484

BLAST of Cp4.1LG01g03300 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 5.1e-74
Identity = 155/434 (35.71%), Postives = 251/434 (57.83%), Query Frame = 0

Query: 32  QQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEW 91
           Q   +Y ++S +       A  LNQ+   G+ + K+EL R +KELRKY+R + AL++ +W
Sbjct: 65  QWNAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDW 124

Query: 92  MEMR--KINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELM 151
           M  R  +   S +D A++LDLI KV+GI  AE +F  L  + K+R  YG+LLN Y +   
Sbjct: 125 MNNRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKS 184

Query: 152 EEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 211
            EKA AL   + +  +A + L FN +MT+YM + + +KV  ++ EMK++ I L  Y+YN+
Sbjct: 185 REKAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNI 244

Query: 212 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 271
           W++SC SL  V K+E + ++MK++     +WTTFS +A +Y+K G+ EKAE AL+KVE  
Sbjct: 245 WLSSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEAR 304

Query: 272 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 331
           I     ++R+ YH+L+SLY S  N+ E+YR+W+  KSV P   N+ Y  ++ +L ++ D 
Sbjct: 305 ITG---RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDI 364

Query: 332 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMF 391
           EG +  Y+EW    SS+D R+ ++ + AY++ D  + A  +F+  ++    P     E+ 
Sbjct: 365 EGAEKVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEIL 424

Query: 392 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 451
            +   + + +  AL+ L +A S      W P   M + F    EEE DV   E    +L+
Sbjct: 425 AVGHTRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLR 484

Query: 452 RFKCLDASAYHLLL 463
           +   L+  +Y  L+
Sbjct: 485 QSGDLEDKSYLALI 495

BLAST of Cp4.1LG01g03300 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-65
Identity = 159/458 (34.72%), Postives = 255/458 (55.68%), Query Frame = 0

Query: 39  RLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRK-I 98
           RL  L  T  S   T+ ++  EG  V+KYEL R ++ELRK +RY HAL+I EWM +++ I
Sbjct: 66  RLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMVVQEDI 125

Query: 99  NYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALS 158
                DYA+ LDLISK++G+ +AE +F D+    +      +LL+ Y +  + +KA AL 
Sbjct: 126 KLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDKAEALF 185

Query: 159 KKIDELKF-ASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASL 218
           +K+ E  F  S L +N++++MY+   Q EKVP LI E+K R       TYN+W+ + AS 
Sbjct: 186 EKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELKIR-TSPDIVTYNLWLTAFASG 245

Query: 219 NGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQD 278
           N V   E++  + K E  N  DW T+S L  +Y K   +EKA LALK++E   K   +++
Sbjct: 246 NDVEGAEKVYLKAKEEKLNP-DWVTYSVLTNLYAKTDNVEKARLALKEME---KLVSKKN 305

Query: 279 RLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYK 338
           R+AY  LISL+A+  ++  V   W  +KS +   N+  YL M+ A+ KL +FE  K  Y 
Sbjct: 306 RVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKGLYD 365

Query: 339 EWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFK 398
           EWES   + D R+ ++ +  Y+ +D        +E  +++   P +   E+    +LK K
Sbjct: 366 EWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYLKRK 425

Query: 399 QVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDAS 458
            ++  L     AI      +W  +  +        EE+ +V+GAE    +L++   ++  
Sbjct: 426 DMEKVLDCFGKAIDSV--KKWTVNVRLVKGACKELEEQGNVKGAEKLMTLLQKAGYVNTQ 485

Query: 459 AYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELL 495
            Y+ LL+TYA AG+ A  + +R+ +DN+E+  E +EL+
Sbjct: 486 LYNSLLRTYAKAGEMALIVEERMAKDNVELDEETKELI 516

BLAST of Cp4.1LG01g03300 vs. NCBI nr
Match: KAG7030878.1 (Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 492/498 (98.80%), Postives = 493/498 (99.00%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AES RGPVNDQQ RLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQ-RLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480

Query: 481 EDNIEVSSELEELLSRQH 498
           EDNIEVSSELEELLSRQH
Sbjct: 481 EDNIEVSSELEELLSRQH 497

BLAST of Cp4.1LG01g03300 vs. NCBI nr
Match: XP_023512291.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Cp4.1LG01g03300 vs. NCBI nr
Match: XP_022942939.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 479/485 (98.76%), Postives = 481/485 (99.18%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AES RGPVNDQQ RLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQ-RLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMY+DAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYEDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKD+EGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDIEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 484

BLAST of Cp4.1LG01g03300 vs. NCBI nr
Match: KAG6600219.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 479/485 (98.76%), Postives = 481/485 (99.18%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRR+LLSRASAGLRHLCTS AES RGPVNDQQ RLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRNLLSRASAGLRHLCTSTAESKRGPVNDQQ-RLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 484

BLAST of Cp4.1LG01g03300 vs. NCBI nr
Match: XP_023005130.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 475/485 (97.94%), Postives = 479/485 (98.76%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AE +RGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSAAELTRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMK+EDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKDEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVK GQLEKAELALKKVENEIKS++QQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKVGQLEKAELALKKVENEIKSSRQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLK TYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKRTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDA LVFEDA KRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAVLVFEDANKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Cp4.1LG01g03300 vs. ExPASy TrEMBL
Match: A0A6J1FST4 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447825 PE=4 SV=1)

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 479/485 (98.76%), Postives = 481/485 (99.18%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AES RGPVNDQQ RLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQ-RLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMY+DAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYEDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKD+EGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDIEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 484

BLAST of Cp4.1LG01g03300 vs. ExPASy TrEMBL
Match: A0A6J1KWK9 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498213 PE=4 SV=1)

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 475/485 (97.94%), Postives = 479/485 (98.76%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AE +RGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSAAELTRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMK+EDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKDEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVK GQLEKAELALKKVENEIKS++QQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKVGQLEKAELALKKVENEIKSSRQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLK TYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKRTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDA LVFEDA KRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAVLVFEDANKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Cp4.1LG01g03300 vs. ExPASy TrEMBL
Match: A0A6J1CK87 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011748 PE=4 SV=1)

HSP 1 Score: 842 bits (2176), Expect = 6.01e-306
Identity = 434/494 (87.85%), Postives = 459/494 (92.91%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSL+S   AGLR LCTS AE +RGP+NDQ+ RLYPRLS LGA+GGSVA+TLNQ+IME
Sbjct: 1   MNRRSLIS--PAGLRQLCTSAAELTRGPLNDQR-RLYPRLSALGASGGSVARTLNQFIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMR IN SFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRNINSSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           E+YFCDLS SAKNRFTYGALLNCYCKE+M+EKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ESYFCDLSPSAKNRFTYGALLNCYCKEMMQEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           M+QPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASL+ VGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MNQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLDDVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVK GQL+KAELALKKVE EIKSNKQ+  LAYHFLISLYASTSN SEV RIW
Sbjct: 241 TFSNLAAIYVKVGQLDKAELALKKVEEEIKSNKQKC-LAYHFLISLYASTSNLSEVNRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
            ALKSVYPMTNN+SYLV+LQALSKLKDF+GLK TY EWESSCS FD RLA VTIGAYLRQ
Sbjct: 301 KALKSVYPMTNNISYLVILQALSKLKDFDGLKKTYNEWESSCSIFDFRLAAVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDAAL+FEDAIKRSKGPF RAREMFMIYFLK KQVD ALSHLESAISES D+EWHPS
Sbjct: 361 DMYKDAALIFEDAIKRSKGPFLRAREMFMIYFLKLKQVDSALSHLESAISESKDNEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480
           PAM NAFL YFEEEKDV+GAEDFA+ILK F CLD+SAYHLLLKTY AAGK +PDMRQRLK
Sbjct: 421 PAMVNAFLKYFEEEKDVKGAEDFAKILKSFNCLDSSAYHLLLKTYVAAGKSSPDMRQRLK 480

Query: 481 EDNIEVSSELEELL 494
           ED IE+SSELEELL
Sbjct: 481 EDKIEISSELEELL 490

BLAST of Cp4.1LG01g03300 vs. ExPASy TrEMBL
Match: A0A6J1FX64 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447825 PE=4 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 3.51e-283
Identity = 394/397 (99.24%), Postives = 396/397 (99.75%), Query Frame = 0

Query: 89  MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL 148
           MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL
Sbjct: 1   MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL 60

Query: 149 MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 208
           MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV
Sbjct: 61  MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 120

Query: 209 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 268
           WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE
Sbjct: 121 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 180

Query: 269 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 328
           IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF
Sbjct: 181 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 240

Query: 329 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMF 388
           EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMY+DAALVFEDAIKRSKGPFFRAREMF
Sbjct: 241 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYEDAALVFEDAIKRSKGPFFRAREMF 300

Query: 389 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 448
           MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKD+EGAEDFARILK
Sbjct: 301 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDIEGAEDFARILK 360

Query: 449 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIE 485
           RFKCLDASAYHLLLKTYAAAGKRAPDMRQRL EDNIE
Sbjct: 361 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIE 397

BLAST of Cp4.1LG01g03300 vs. ExPASy TrEMBL
Match: A0A6J1KSI4 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498213 PE=4 SV=1)

HSP 1 Score: 774 bits (1999), Expect = 3.90e-280
Identity = 390/397 (98.24%), Postives = 393/397 (98.99%), Query Frame = 0

Query: 89  MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL 148
           MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL
Sbjct: 1   MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL 60

Query: 149 MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 208
           MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV
Sbjct: 61  MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 120

Query: 209 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 268
           WMNSCASLNGVGKVEEILEEMK+EDRNKFDWTTFSNLAAIYVK GQLEKAELALKKVENE
Sbjct: 121 WMNSCASLNGVGKVEEILEEMKDEDRNKFDWTTFSNLAAIYVKVGQLEKAELALKKVENE 180

Query: 269 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 328
           IKS++QQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF
Sbjct: 181 IKSSRQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 240

Query: 329 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMF 388
           EGLK TYKEWESSCSSFDLRLADVTIGAYLRQDMYKDA LVFEDA KRSKGPFFRAREMF
Sbjct: 241 EGLKRTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAVLVFEDANKRSKGPFFRAREMF 300

Query: 389 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 448
           MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK
Sbjct: 301 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 360

Query: 449 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIE 485
           RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIE
Sbjct: 361 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIE 397

BLAST of Cp4.1LG01g03300 vs. TAIR 10
Match: AT1G02370.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 485.7 bits (1249), Expect = 4.4e-137
Identity = 248/470 (52.77%), Postives = 339/470 (72.13%), Query Frame = 0

Query: 29  VNDQQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQI 88
           V  +Q+ LY +LS L  TGG+VA+TLNQ+IMEG  V+K +L RC K LRK+RR  HA +I
Sbjct: 66  VASRQRELYKKLSMLSVTGGTVAETLNQFIMEGITVRKDDLFRCAKTLRKFRRPQHAFEI 125

Query: 89  MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKN-RFTYGALLNCYCKE 148
            +WME RK+ +S +D+A+ LDLI K KG+ AAENYF +L  SAKN + TYGAL+NCYC E
Sbjct: 126 FDWMEKRKMTFSVSDHAICLDLIGKTKGLEAAENYFNNLDPSAKNHQSTYGALMNCYCVE 185

Query: 149 LMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTY 208
           L EEKA A  + +DEL F +N L FNN+M+MYMR+ QPEKVP L+D MK+RGI     TY
Sbjct: 186 LEEEKAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPEKVPVLVDAMKQRGISPCGVTY 245

Query: 209 NVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVE 268
           ++WM SC SLN +  +E+I++EM  +   K  W TFSNLAAIY KAG  EKA+ ALK +E
Sbjct: 246 SIWMQSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNLAAIYTKAGLYEKADSALKSME 305

Query: 269 NEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLK 328
            ++  N   +R ++HFL+SLYA  S   EVYR+W +LK   P  NN+SYLVMLQA+SKL 
Sbjct: 306 EKMNPN---NRDSHHFLMSLYAGISKGPEVYRVWESLKKARPEVNNLSYLVMLQAMSKLG 365

Query: 329 DFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRARE 388
           D +G+K  + EWES C ++D+RLA++ I  YL+ +MY++A  + + A+K+SKGPF +AR+
Sbjct: 366 DLDGIKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEKILDGAMKKSKGPFSKARQ 425

Query: 389 MFMIYFLKFKQVDLALSHLESAISESMD--DEWHPSPAMANAFLMYFEEEKDVEGAEDFA 448
           + MI+ L+  + DLA+ HLE+A+S+S +  DEW  S  + + F ++FE+ KDV+GAEDF 
Sbjct: 426 LLMIHLLENDKADLAMKHLEAAVSDSAENKDEWGWSSELVSLFFLHFEKAKDVDGAEDFC 485

Query: 449 RILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELL 495
           +IL  +K LD+     L+KTYAAA K +PDMR+RL +  IEVS E+++LL
Sbjct: 486 KILSNWKPLDSETMTFLIKTYAAAEKTSPDMRERLSQQQIEVSEEIQDLL 532

BLAST of Cp4.1LG01g03300 vs. TAIR 10
Match: AT4G01990.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 436.8 bits (1122), Expect = 2.3e-122
Identity = 229/467 (49.04%), Postives = 318/467 (68.09%), Query Frame = 0

Query: 32  QQQRLYPRLSKLGAT-GGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIME 91
           + + +Y +LS LG   GG + +TLNQ++MEG  VKK++L R  K+LRK+R+   AL+I E
Sbjct: 37  KHRSIYKKLSSLGTRGGGKMEETLNQFVMEGVPVKKHDLIRYAKDLRKFRQPQRALEIFE 96

Query: 92  WMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELME 151
           WME ++I ++ +D+A+RL+LI+K KG+ AAE YF  L  S KN+ TYG+LLNCYC E  E
Sbjct: 97  WMERKEIAFTGSDHAIRLNLIAKSKGLEAAETYFNSLDDSIKNQSTYGSLLNCYCVEKEE 156

Query: 152 EKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNVW 211
            KA A  + + +L   SN L FNNLM MYM + QPEKVP L+  MK + I     TY++W
Sbjct: 157 VKAKAHFENMVDLNHVSNSLPFNNLMAMYMGLGQPEKVPALVVAMKEKSITPCDITYSMW 216

Query: 212 MNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEI 271
           + SC SL  +  VE++L+EMK E    F W TF+NLAAIY+K G   KAE ALK +EN +
Sbjct: 217 IQSCGSLKDLDGVEKVLDEMKAEGEGIFSWNTFANLAAIYIKVGLYGKAEEALKSLENNM 276

Query: 272 KSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFE 331
             +    R  YHFLI+LY   +N SEVYR+W+ LK  YP  NN SYL ML+ALSKL D +
Sbjct: 277 NPDV---RDCYHFLINLYTGIANASEVYRVWDLLKKRYPNVNNSSYLTMLRALSKLDDID 336

Query: 332 GLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFM 391
           G+K  + EWES+C ++D+R+A+V I +YL+Q+MY++A  VF  A+K+ KG F +AR++ M
Sbjct: 337 GVKKVFAEWESTCWTYDMRMANVAISSYLKQNMYEEAEAVFNGAMKKCKGQFSKARQLLM 396

Query: 392 IYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKR 451
           ++ LK  Q DLAL H E+A+ +  D  W  S  + ++F ++FEE KDV+GAE+F + L +
Sbjct: 397 MHLLKNDQADLALKHFEAAVLD-QDKNWTWSSELISSFFLHFEEAKDVDGAEEFCKTLTK 456

Query: 452 FKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELLSR 497
           +  L +  Y LL+KTY AAGK  PDM++RL+E  I V  E E LLS+
Sbjct: 457 WSPLSSETYTLLMKTYLAAGKACPDMKKRLEEQGILVDEEQECLLSK 499

BLAST of Cp4.1LG01g03300 vs. TAIR 10
Match: AT1G60770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-102
Identity = 206/485 (42.47%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 14  LRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCI 73
           +RHL  S   + R      ++ LY RL K G T   V Q LNQ++   K V K+E+   I
Sbjct: 3   MRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFKWEVGDTI 62

Query: 74  KELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKN 133
           K+LR    Y+ AL++ E ME R +N + +D A+ LDL++K + I A ENYF DL  ++K 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERGMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSKT 122

Query: 134 RFTYGALLNCYCKELMEEKALALSKKIDELKFA-SNLSFNNLMTMYMRMDQPEKVPPLID 193
             TYG+LLNCYCKEL+ EKA  L  K+ EL    S++S+N+LMT+Y +  + EKVP +I 
Sbjct: 123 ELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMIQ 182

Query: 194 EMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKA 253
           E+K   +   +YTYNVWM + A+ N +  VE ++EEM  + R   DWTT+SN+A+IYV A
Sbjct: 183 ELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVDA 242

Query: 254 GQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNN 313
           G  +KAE AL+++E     N Q+D  AY FLI+LY      +EVYRIW +L+   P T+N
Sbjct: 243 GLSQKAEKALQELE---MKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSN 302

Query: 314 MSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFED 373
           ++YL M+Q L KL D  G ++ +KEW+++CS++D+R+ +V IGAY ++ + + A  + E 
Sbjct: 303 VAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEK 362

Query: 374 AIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDD--EWHPSPAMANAFLMY 433
           A +R      +  E+FM Y++K   +  AL  +  A+S    D  +W PSP    A + Y
Sbjct: 363 APRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMSY 422

Query: 434 FEEEKDVEGAEDFARILKR-FKCLDASAYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSE 493
           FE++KDV GAE+   ILK     + A  +  L++TYAAAGK  P MR+RLK +N+EV+  
Sbjct: 423 FEQKKDVNGAENLLEILKNGTDNIGAEIFEPLIRTYAAAGKSHPAMRRRLKMENVEVNEA 482

Query: 494 LEELL 495
            ++LL
Sbjct: 483 TKKLL 484

BLAST of Cp4.1LG01g03300 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.6e-75
Identity = 155/434 (35.71%), Postives = 251/434 (57.83%), Query Frame = 0

Query: 32  QQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEW 91
           Q   +Y ++S +       A  LNQ+   G+ + K+EL R +KELRKY+R + AL++ +W
Sbjct: 65  QWNAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDW 124

Query: 92  MEMR--KINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELM 151
           M  R  +   S +D A++LDLI KV+GI  AE +F  L  + K+R  YG+LLN Y +   
Sbjct: 125 MNNRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKS 184

Query: 152 EEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 211
            EKA AL   + +  +A + L FN +MT+YM + + +KV  ++ EMK++ I L  Y+YN+
Sbjct: 185 REKAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNI 244

Query: 212 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 271
           W++SC SL  V K+E + ++MK++     +WTTFS +A +Y+K G+ EKAE AL+KVE  
Sbjct: 245 WLSSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEAR 304

Query: 272 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 331
           I     ++R+ YH+L+SLY S  N+ E+YR+W+  KSV P   N+ Y  ++ +L ++ D 
Sbjct: 305 ITG---RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDI 364

Query: 332 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMF 391
           EG +  Y+EW    SS+D R+ ++ + AY++ D  + A  +F+  ++    P     E+ 
Sbjct: 365 EGAEKVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEIL 424

Query: 392 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 451
            +   + + +  AL+ L +A S      W P   M + F    EEE DV   E    +L+
Sbjct: 425 AVGHTRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLR 484

Query: 452 RFKCLDASAYHLLL 463
           +   L+  +Y  L+
Sbjct: 485 QSGDLEDKSYLALI 495

BLAST of Cp4.1LG01g03300 vs. TAIR 10
Match: AT4G02820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 8.1e-67
Identity = 159/458 (34.72%), Postives = 255/458 (55.68%), Query Frame = 0

Query: 39  RLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRK-I 98
           RL  L  T  S   T+ ++  EG  V+KYEL R ++ELRK +RY HAL+I EWM +++ I
Sbjct: 66  RLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMVVQEDI 125

Query: 99  NYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALS 158
                DYA+ LDLISK++G+ +AE +F D+    +      +LL+ Y +  + +KA AL 
Sbjct: 126 KLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDKAEALF 185

Query: 159 KKIDELKF-ASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASL 218
           +K+ E  F  S L +N++++MY+   Q EKVP LI E+K R       TYN+W+ + AS 
Sbjct: 186 EKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELKIR-TSPDIVTYNLWLTAFASG 245

Query: 219 NGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQD 278
           N V   E++  + K E  N  DW T+S L  +Y K   +EKA LALK++E   K   +++
Sbjct: 246 NDVEGAEKVYLKAKEEKLNP-DWVTYSVLTNLYAKTDNVEKARLALKEME---KLVSKKN 305

Query: 279 RLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYK 338
           R+AY  LISL+A+  ++  V   W  +KS +   N+  YL M+ A+ KL +FE  K  Y 
Sbjct: 306 RVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKGLYD 365

Query: 339 EWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFK 398
           EWES   + D R+ ++ +  Y+ +D        +E  +++   P +   E+    +LK K
Sbjct: 366 EWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYLKRK 425

Query: 399 QVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDAS 458
            ++  L     AI      +W  +  +        EE+ +V+GAE    +L++   ++  
Sbjct: 426 DMEKVLDCFGKAIDSV--KKWTVNVRLVKGACKELEEQGNVKGAEKLMTLLQKAGYVNTQ 485

Query: 459 AYHLLLKTYAAAGKRAPDMRQRLKEDNIEVSSELEELL 495
            Y+ LL+TYA AG+ A  + +R+ +DN+E+  E +EL+
Sbjct: 486 LYNSLLRTYAKAGEMALIVEERMAKDNVELDEETKELI 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ246.2e-13652.77Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidop... [more]
Q93WC53.3e-12149.04Pentatricopeptide repeat-containing protein At4g01990, mitochondrial OS=Arabidop... [more]
O227142.9e-10142.47Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX... [more]
Q8LPS65.1e-7435.71Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
Q9SY071.1e-6534.72Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
KAG7030878.10.098.80Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosper... [more]
XP_023512291.10.0100.00pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
XP_022942939.10.098.76pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
KAG6600219.10.098.76Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_023005130.10.097.94pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
Match NameE-valueIdentityDescription
A0A6J1FST40.098.76pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A6J1KWK90.097.94pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A6J1CK876.01e-30687.85pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Momordic... [more]
A0A6J1FX643.51e-28399.24pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A6J1KSI43.90e-28098.24pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
Match NameE-valueIdentityDescription
AT1G02370.14.4e-13752.77Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G01990.12.3e-12249.04Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G60770.12.1e-10242.47Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.13.6e-7535.71Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G02820.18.1e-6734.72Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 255..275
NoneNo IPR availableCOILSCoilCoilcoord: 476..496
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 4..496
NoneNo IPR availablePANTHERPTHR45717:SF8OS01G0301500 PROTEINcoord: 4..496
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 170..214
e-value: 5.8E-9
score: 36.0
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 135..156
e-value: 0.034
score: 14.4
coord: 241..264
e-value: 1.4
score: 9.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 167..201
score: 8.506026
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 269..442
e-value: 1.1E-8
score: 36.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 49..167
e-value: 3.9E-6
score: 28.3
coord: 168..268
e-value: 5.9E-12
score: 47.3
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 134..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g03300.1Cp4.1LG01g03300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding