Cp4.1LG01g02260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g02260
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionVID27 domain-containing protein
LocationCp4.1LG01: 2578032 .. 2581143 (+)
RNA-Seq ExpressionCp4.1LG01g02260
SyntenyCp4.1LG01g02260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTGAATCTCAGATTAATTAACTACCCTAAAATTTGTCTCCCAAACCTTCTGGGTCTCAAAAGAGTTCCAATTATCGCGATCAAATTTCTTGAAATTCCTCCTGATTTTCTCTTCCTAGTTCCTCCAATCCATCCCGGAATCGGAATCGGAATCGTGTTTTCCCCATTTGCAGATCACACGGTCCGAAATGGGTACCGCGCATAGCCGCGACTGCGAGGATGTTGAACTTTCAGACTCCGATGAATCCAAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAATGCGACGAGGCTGGGACGGAATTGAAGCCAGCTAAATCGGCTGGTACTTCATCTGCAATTGATGATGTCGACGCCAAACTCAAAGCTCTTAACCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAATGCTGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTTTCTGAGAAACTCACTTCCTACGATTTTCTCAAAACGGCGAACGTCGACGGAAATAATTACGACGACGAGGAGGAAGATGACGTTAGGGGTTACAAAGCCAAATCGTCGGGAGGGAGAGATCAGTTGCGTTGGGTACTCCAAGTAGGGGCGAAGATTAGAGCTCTGGTTTCGACAGAAATGCAAATGAAGATGTTCGGCGATCAGCGGCGTGTCGATTTTGTTGACAAAGGCGTTTGGGCTTTGAAGTTCCCGAGCGATGAACAGTATCGGAATTTCGTAACTGAATTCCAAGATTGCTTGTTTGAGAATGTCTATGGTCTTCAAGCGACTGATGAGAACAGGGTCAAGATTTACGGGAAGGAATTCATTGGGTGGTTGAAGCCGGAAGTAGCCGATGATTCGATTTGGGAAAATGCTGATATTGAATTCGGGAAGAGCCCTAGGTCGAAGACTCCGTCGGTGAGAACGAAGCACGACTTGATAGAGGAGTTCGAGGAAGCTGCTAATGGAGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAATGATTCAGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCACGGGAAGGGGGTCTCGTTGAAAGTTGGTTCAGGAAATTCCCCAAACATTGGTCGATCAACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAGATAGTCACCGAATGGAAGTTTGAAAAGGATGGTAATGATATTACAATGAGGGACATCACGAACAACACCAAAGGATCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGCTGGATGATAACAAGCTTTGTCAATGGGACATGAGAGACCGTAAAGGAAAGGTTCAGAACATTGGAGGTGCTGATAATTCACCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTGTAGAGGGACAAACTTCCAGTGCTTTGCTACGACAGGGGATGGGTCGATTGTTGTTGGGTCAGCTGATGGAAAGATTAGGCTGTATTCGAAGGCGTCGATGAGACAGGCGAAGACAGCTTTTCCAGGGCTCGGTTCGTCGATTAATCATGTGGATGTTACTTATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATTTGCACATTATTCACTGATAAAGATGGGAACATGAAAACTGGGTTCAGTGGTCGGATGGGCAACAGGATTCCAGCTCCGAGGTTGCTGAAATTGACTCCCCTTGATTCCCATTTGGCTGGAGCAGACAACAAATTCCATGGTGGCCACTTTTCATGGGTAAGAAAAACTAGTTCTGAATTTGATCGCTTGAATCTTGCTCTGTTTTGTAGCTGGCTTGGTTGGTTGGTTGCTGTTGCTTAAGGTTTTATTAGTCTTTTGGCTAAATATATAATTTCGATGCTAATGAAATGGACATTGAATTGAAAATTTATCTGCTTCAACTCACCTGCAACTTTTATGGCCCAATCCCACCGTTAGTAGATATTGTCTTCTTTGGGCTTTCCCTTTTAGACTTTCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGGGGTTTCCACACCCTTCGTTCCCCTCTCCAACCGATGTGGGATCTCACAGTTTACCCCCTTCAGAGCCCAGTGTTCTCGTTGACACACCGCTCGGTGTCTGGCTCTAGTTCCATCTGTCCCACCGTTAGCAGATATTGTCCTCTTTGAGCTTTCCCTTTCAGGCTTCCCCTCAAGGTTTTTAAAACGTGTCTACTAGGGAGAGATTTCCACACCCTTACAAAGAATGTTTCATTCCCCTATTACCCAACAAAACTTGATGGGCCGTTGAGGTAGAAAATGGGAACCCTCGAGTAGTGAAGAAGATGATGGCGATTCTCGTCCTTTTCAACTATGATGTGATAGCAATCTCTTACTAACTTATTAGAGACGGGAGTAGAAAAGTGGGTGTCTTTGAAGATATATCAGTAGTTTTTTTGGTTTATGTTCACGTCATGTTGGTTGCTCTATCATAGCTTCTGTTATTCCTACAAGGAATTTCCTTCATTACTTCAATTCATACAAAACTGGCATTTGAGTTTCTTTTCTTTTGCCTTCAGTCCGCAAGTTCCATACATGACCTTACAATCTTATATCGACAACGATTTTCAGGTCACCGAGAGCGGGAAACAAGAGCGATACCTCGTTGCAACAGTGGGGAAGTTCAGTGTAATATGGGACTTCCATCAAGTAAAGAACAGTGCACACGAATGCTACCGAAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGTTGAAGGATGAATCCATCGTCGAAAGTCGGTTCATGCACGACAAGTTTGCTGTCTTAGATTCTCCAGAAGCTCCACTGGTTGTGGCTACGCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGGAAGAGATGAGGCGCCATTGTTAACATGTCGACGATGTGCTTTCTCGTCTCCATTGATTGATGTGTTCCTGTAGCTATACTGCTGCTGCTGCTGCTGCTTTCTTTGTTAATCCTCCCTTCATAT

mRNA sequence

ACCTGAATCTCAGATTAATTAACTACCCTAAAATTTGTCTCCCAAACCTTCTGGGTCTCAAAAGAGTTCCAATTATCGCGATCAAATTTCTTGAAATTCCTCCTGATTTTCTCTTCCTAGTTCCTCCAATCCATCCCGGAATCGGAATCGGAATCGTGTTTTCCCCATTTGCAGATCACACGGTCCGAAATGGGTACCGCGCATAGCCGCGACTGCGAGGATGTTGAACTTTCAGACTCCGATGAATCCAAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAATGCGACGAGGCTGGGACGGAATTGAAGCCAGCTAAATCGGCTGGTACTTCATCTGCAATTGATGATGTCGACGCCAAACTCAAAGCTCTTAACCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAATGCTGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTTTCTGAGAAACTCACTTCCTACGATTTTCTCAAAACGGCGAACGTCGACGGAAATAATTACGACGACGAGGAGGAAGATGACGTTAGGGGTTACAAAGCCAAATCGTCGGGAGGGAGAGATCAGTTGCGTTGGGTACTCCAAGTAGGGGCGAAGATTAGAGCTCTGGTTTCGACAGAAATGCAAATGAAGATGTTCGGCGATCAGCGGCGTGTCGATTTTGTTGACAAAGGCGTTTGGGCTTTGAAGTTCCCGAGCGATGAACAGTATCGGAATTTCGTAACTGAATTCCAAGATTGCTTGTTTGAGAATGTCTATGGTCTTCAAGCGACTGATGAGAACAGGGTCAAGATTTACGGGAAGGAATTCATTGGGTGGTTGAAGCCGGAAGTAGCCGATGATTCGATTTGGGAAAATGCTGATATTGAATTCGGGAAGAGCCCTAGGTCGAAGACTCCGTCGGTGAGAACGAAGCACGACTTGATAGAGGAGTTCGAGGAAGCTGCTAATGGAGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAATGATTCAGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCACGGGAAGGGGGTCTCGTTGAAAGTTGGTTCAGGAAATTCCCCAAACATTGGTCGATCAACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAGATAGTCACCGAATGGAAGTTTGAAAAGGATGGTAATGATATTACAATGAGGGACATCACGAACAACACCAAAGGATCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGCTGGATGATAACAAGCTTTGTCAATGGGACATGAGAGACCGTAAAGGAAAGGTTCAGAACATTGGAGGTGCTGATAATTCACCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTGTAGAGGGACAAACTTCCAGTGCTTTGCTACGACAGGGGATGGGTCGATTGTTGTTGGGTCAGCTGATGGAAAGATTAGGCTGTATTCGAAGGCGTCGATGAGACAGGCGAAGACAGCTTTTCCAGGGCTCGGTTCGTCGATTAATCATGTGGATGTTACTTATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATTTGCACATTATTCACTGATAAAGATGGGAACATGAAAACTGGGTTCAGTGGTCGGATGGGCAACAGGATTCCAGCTCCGAGGTTGCTGAAATTGACTCCCCTTGATTCCCATTTGGCTGGAGCAGACAACAAATTCCATGGTGGCCACTTTTCATGGGTCACCGAGAGCGGGAAACAAGAGCGATACCTCGTTGCAACAGTGGGGAAGTTCAGTGTAATATGGGACTTCCATCAAGTAAAGAACAGTGCACACGAATGCTACCGAAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGTTGAAGGATGAATCCATCGTCGAAAGTCGGTTCATGCACGACAAGTTTGCTGTCTTAGATTCTCCAGAAGCTCCACTGGTTGTGGCTACGCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGGAAGAGATGAGGCGCCATTGTTAACATGTCGACGATGTGCTTTCTCGTCTCCATTGATTGATGTGTTCCTGTAGCTATACTGCTGCTGCTGCTGCTGCTTTCTTTGTTAATCCTCCCTTCATAT

Coding sequence (CDS)

ATGGGTACCGCGCATAGCCGCGACTGCGAGGATGTTGAACTTTCAGACTCCGATGAATCCAAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAATGCGACGAGGCTGGGACGGAATTGAAGCCAGCTAAATCGGCTGGTACTTCATCTGCAATTGATGATGTCGACGCCAAACTCAAAGCTCTTAACCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAATGCTGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTTTCTGAGAAACTCACTTCCTACGATTTTCTCAAAACGGCGAACGTCGACGGAAATAATTACGACGACGAGGAGGAAGATGACGTTAGGGGTTACAAAGCCAAATCGTCGGGAGGGAGAGATCAGTTGCGTTGGGTACTCCAAGTAGGGGCGAAGATTAGAGCTCTGGTTTCGACAGAAATGCAAATGAAGATGTTCGGCGATCAGCGGCGTGTCGATTTTGTTGACAAAGGCGTTTGGGCTTTGAAGTTCCCGAGCGATGAACAGTATCGGAATTTCGTAACTGAATTCCAAGATTGCTTGTTTGAGAATGTCTATGGTCTTCAAGCGACTGATGAGAACAGGGTCAAGATTTACGGGAAGGAATTCATTGGGTGGTTGAAGCCGGAAGTAGCCGATGATTCGATTTGGGAAAATGCTGATATTGAATTCGGGAAGAGCCCTAGGTCGAAGACTCCGTCGGTGAGAACGAAGCACGACTTGATAGAGGAGTTCGAGGAAGCTGCTAATGGAGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAATGATTCAGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCACGGGAAGGGGGTCTCGTTGAAAGTTGGTTCAGGAAATTCCCCAAACATTGGTCGATCAACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAGATAGTCACCGAATGGAAGTTTGAAAAGGATGGTAATGATATTACAATGAGGGACATCACGAACAACACCAAAGGATCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGCTGGATGATAACAAGCTTTGTCAATGGGACATGAGAGACCGTAAAGGAAAGGTTCAGAACATTGGAGGTGCTGATAATTCACCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTGTAGAGGGACAAACTTCCAGTGCTTTGCTACGACAGGGGATGGGTCGATTGTTGTTGGGTCAGCTGATGGAAAGATTAGGCTGTATTCGAAGGCGTCGATGAGACAGGCGAAGACAGCTTTTCCAGGGCTCGGTTCGTCGATTAATCATGTGGATGTTACTTATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATTTGCACATTATTCACTGATAAAGATGGGAACATGAAAACTGGGTTCAGTGGTCGGATGGGCAACAGGATTCCAGCTCCGAGGTTGCTGAAATTGACTCCCCTTGATTCCCATTTGGCTGGAGCAGACAACAAATTCCATGGTGGCCACTTTTCATGGGTCACCGAGAGCGGGAAACAAGAGCGATACCTCGTTGCAACAGTGGGGAAGTTCAGTGTAATATGGGACTTCCATCAAGTAAAGAACAGTGCACACGAATGCTACCGAAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGTTGAAGGATGAATCCATCGTCGAAAGTCGGTTCATGCACGACAAGTTTGCTGTCTTAGATTCTCCAGAAGCTCCACTGGTTGTGGCTACGCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGGAAGAGATGA

Protein sequence

MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR
Homology
BLAST of Cp4.1LG01g02260 vs. ExPASy Swiss-Prot
Match: P40781 (Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.1e-224
Identity = 379/494 (76.72%), Postives = 423/494 (85.63%), Query Frame = 0

Query: 161 MFGDQRRVDFVDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFI 220
           MFGDQRRVDFVD GVWALKF  DE YR FVT FQ CLFENVYGL+A+DEN+VK+YGK+FI
Sbjct: 1   MFGDQRRVDFVDSGVWALKFMRDEDYREFVTRFQSCLFENVYGLKASDENKVKVYGKDFI 60

Query: 221 GWLKPEVADDSIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAA-NGGVQSLTLGALDN 280
           GW+KP+VADDS+WE  D E  +SP   TP VR  +DL+EEFEEAA +GG+QS+ LGALDN
Sbjct: 61  GWVKPDVADDSMWEKDDDELWRSPSKMTP-VRGSNDLLEEFEEAASDGGIQSVALGALDN 120

Query: 281 SFLVNDSGVQVYKNLSHGIHGKGVSLK-------VGSGNSPNIGRSTPKKGLLMKAETNM 340
           SFLVNDSGVQV KN SHGIHGKGV +K       VG  +S      TP+K LLM+ ETNM
Sbjct: 121 SFLVNDSGVQVVKNFSHGIHGKGVYVKFDNGGKRVGGSSSGGYSNLTPQKALLMRGETNM 180

Query: 341 LLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATF 400
           LLMSP K GKPH++G+ QLDIETGKIVTEWKFEKDG DITMRDITN+TKGSQLDPSE+TF
Sbjct: 181 LLMSPAKAGKPHSTGVNQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTF 240

Query: 401 LGLDDNKLCQWDMRDRKGKVQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVG 460
           LGLDDN+L QWDMRDR+G VQNI  +D SPVL+W QGHQF RGTNFQ FATTGDGSIVVG
Sbjct: 241 LGLDDNRLSQWDMRDRRGMVQNIAHSD-SPVLHWTQGHQFSRGTNFQSFATTGDGSIVVG 300

Query: 461 SADGKIRLYSKASMRQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDG 520
           S DGKIRLYS  SMR AKTAFPGLGS I HVDVTYDGKWILGTTDTYLILIC+LFTDKDG
Sbjct: 301 SLDGKIRLYSTTSMRMAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICSLFTDKDG 360

Query: 521 NMKTGFSGRMGNRIPAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGK 580
             KTGFSGRMGN+IPAPRLLKLTP+DSH AG +NKFHGG FSWVTESGKQER+LVATVGK
Sbjct: 361 KTKTGFSGRMGNKIPAPRLLKLTPVDSHTAGVNNKFHGGRFSWVTESGKQERHLVATVGK 420

Query: 581 FSVIWDFHQVKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVL-DSPEAPL 640
           FSVIWDF +VKNS HECYRNQ+GLKSCYCYK++ KDESI+ES FM+DK+A + DSPEAPL
Sbjct: 421 FSVIWDFQRVKNSGHECYRNQEGLKSCYCYKLMTKDESIIESLFMNDKYAAVGDSPEAPL 480

Query: 641 VVATPMKVSSISLS 646
           V+ATP K++S S+S
Sbjct: 481 VMATPKKITSFSMS 492

BLAST of Cp4.1LG01g02260 vs. ExPASy Swiss-Prot
Match: Q555V7 (VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 5.0e-29
Identity = 100/387 (25.84%), Postives = 173/387 (44.70%), Query Frame = 0

Query: 226 EVADDSIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVND 285
           E  +D   E +D +  +   S+      K  + ++F ++ N    SL +G  D S++V  
Sbjct: 551 ESDEDEDEEESDEDEDEESDSEENLSEDKKQISKDFSKSKN---SSLIVGYKDKSYVVRG 610

Query: 286 SGVQVYKNLSHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHA 345
           S + V+     GI        +   +  N    +PKK +L + +  +L+++P K+     
Sbjct: 611 STIGVFNTDDDGIKFNTAITNIKQKDGKNF---SPKKMMLQQQDQTLLMLNPEKK----- 670

Query: 346 SGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDM 405
           S + ++D+    IV EW             + +  K    + +   F+G + N +   D 
Sbjct: 671 SNVYKMDLNRPDIVEEWDMNWRNQPTLTNSVVSQYKNDD-ETNNQLFVGYNGNNMFLVDP 730

Query: 406 RDRKGK--VQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK 465
           R+ K K  V+  GG++   V                C ATTG G I  G++ G+I+L+SK
Sbjct: 731 RESKNKVTVKFHGGSNPKSVTT--------------CAATTGSGQIAFGTSKGEIKLFSK 790

Query: 466 ASMRQ----------------AKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLF 525
               Q                ++T  PG+G  I  +DVT DGKWI+ T   Y+++I    
Sbjct: 791 TQFDQNKRSVTSSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPASL 850

Query: 526 TDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLV 585
             KDG+  +GF  R+G R P+P+ L L P D    G    F    F+ V +  + E  ++
Sbjct: 851 --KDGS--SGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDE-QSETSIL 906

Query: 586 ATVGKFSVIWDFHQVKNSAHECYRNQQ 595
            + G F + W+F ++K +  + Y+ +Q
Sbjct: 911 TSTGSFLITWNFRKIKQNILDVYQIKQ 906

BLAST of Cp4.1LG01g02260 vs. ExPASy Swiss-Prot
Match: Q1MTR3 (Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vid27 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 8.8e-26
Identity = 97/328 (29.57%), Postives = 160/328 (48.78%), Query Frame = 0

Query: 278 DNSFLVNDSGVQVYKNLSHGIHGKGVSLKVGSGN--SPNIGRSTPKKGLLMKAETNMLLM 337
           D S++V ++ + V+K++      KG+  +    N  +P      P K +L   ++++L  
Sbjct: 446 DRSYVVRNNKIGVFKHVDE----KGLKFQTALNNLSTPKGKSLRPSKLMLHNQDSSILFQ 505

Query: 338 SPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGL 397
           +   E  PH+  L  +DIE GKIV EWK     +D+ +   T + K +Q+  +E T +GL
Sbjct: 506 T---ENAPHS--LYHMDIEYGKIVDEWKVH---DDVPLVTFTPDNKFAQM-TAEQTLIGL 565

Query: 398 DDNKLCQWDMRDRKGKVQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSAD 457
            +N + + D R    K            L   Q  Q+    +F   ATT +G I V S  
Sbjct: 566 SNNSIFRIDPRVEGNK------------LVAEQFKQYATKNDFSSAATTENGYIAVASNK 625

Query: 458 GKIRLYSKASMRQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMK 517
           G IRL+ +  +  AKTA P LG +I  VDVT  G ++L T  TY++LI       D  +K
Sbjct: 626 GDIRLFDRIGV-NAKTALPALGEAIIGVDVTASGDFVLATCKTYILLI-------DTRIK 685

Query: 518 TG-FSGRMG-------NRIPAPRLLKLTPLDSHLAGADNKFHGG----HFSWVTESGKQE 577
            G ++GR+G       ++ P P+ L+L+P   H+A    +  GG       + T    +E
Sbjct: 686 EGRYAGRLGFERNFAKDKKPKPKRLQLSP--QHIAMMQRELKGGASFTPAKFNTGIDAKE 738

Query: 578 RYLVATVGKFSVIWDFHQVKNSAHECYR 592
             +V+++G F + W+  +VK    + Y+
Sbjct: 746 TTIVSSIGPFLISWNLDRVKRGFTDSYK 738

BLAST of Cp4.1LG01g02260 vs. ExPASy Swiss-Prot
Match: P40157 (Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VID27 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.0e-18
Identity = 98/373 (26.27%), Postives = 155/373 (41.55%), Query Frame = 0

Query: 256 DLIEEFEEAANGGVQSLTLGALDN-SFLVNDSGVQVYKNLSHGIHGKGVSLKVGSGNSPN 315
           +  EE   A + G  SLT+   +N S++  D+ + V+K          +       N  N
Sbjct: 407 EAFEEPRRATSKGNSSLTVAFRNNRSYVTRDNRIGVFKT---DDEDDSLEFVAAIKNISN 466

Query: 316 IGRST--PKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGNDIT 375
           +G  +  P K +L   + N++L     E K     L ++DIE GK++ EW       D  
Sbjct: 467 LGGKSIDPHKPMLYMEDRNLILTDGENENK-----LYKMDIERGKVIEEW----STGDKN 526

Query: 376 MRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADNSPVLNWAQGHQF 435
           +       K  Q+ P E T +G+    + + D R        I G +   V    +   +
Sbjct: 527 VVQYGPTKKFDQMTP-EQTIVGVSQKGVFKIDPR--------INGKNKIAV---DESKDY 586

Query: 436 CRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSINHVDVTYDGKWI 495
               NF    TT  G I +GS  G I+LY +  +R AKTA P LG +I  +  + DGKW+
Sbjct: 587 VGKYNFSSIGTTESGYIAIGSEKGDIKLYDRLGIR-AKTAIPSLGQAIKFITTSADGKWL 646

Query: 496 LGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPR-----LLKLTPLDSHLAGADNK 555
           L T ++ L+L+     D       GF        PA       +LK+ P  S       K
Sbjct: 647 LATCESTLLLMDLKIKDGKNAGNIGFL----KSFPASENVKTYVLKIRPEHSASILTYTK 706

Query: 556 ----FHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCYCYK 615
               F   +F+  T  G+QE+ +V + G +++ W    +         NQ G  + Y Y+
Sbjct: 707 KPIRFTKAYFN--TGIGQQEQTIVTSTGPYAISWSLKGI--------LNQDGSNN-YPYR 739

Query: 616 IVLKDESIVESRF 617
           I   +  +V   F
Sbjct: 767 IRRYNADVVADNF 739

BLAST of Cp4.1LG01g02260 vs. NCBI nr
Match: XP_023529490.1 (protein CYPRO4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1303 bits (3371), Expect = 0.0
Identity = 648/648 (100.00%), Postives = 648/648 (100.00%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA 60
           MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA
Sbjct: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA 60

Query: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE 120
           LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE
Sbjct: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE 120

Query: 121 EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180
           EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF
Sbjct: 121 EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180

Query: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240
           PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF
Sbjct: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240

Query: 241 GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300
           GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG
Sbjct: 241 GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300

Query: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360
           KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT
Sbjct: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360

Query: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420
           EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN
Sbjct: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420

Query: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI 480
           SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI
Sbjct: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI 480

Query: 481 NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540
           NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH
Sbjct: 481 NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540

Query: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600
           LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY
Sbjct: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600

Query: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648

BLAST of Cp4.1LG01g02260 vs. NCBI nr
Match: XP_022979546.1 (protein CYPRO4 [Cucurbita maxima])

HSP 1 Score: 1273 bits (3295), Expect = 0.0
Identity = 635/650 (97.69%), Postives = 640/650 (98.46%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEE--CDEAGTELKPAKSAGTSSAIDDVDAKL 60
           MGTAHSRDCEDVELSDSDE KEEEEEEEEEE  CDEAGTELK  KS GTSSAIDDVDAKL
Sbjct: 1   MGTAHSRDCEDVELSDSDEFKEEEEEEEEEEEECDEAGTELKLDKSTGTSSAIDDVDAKL 60

Query: 61  KALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD 120
           KALNLKYGSSSP QIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD
Sbjct: 61  KALNLKYGSSSPYQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD 120

Query: 121 DEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL 180
           DEEEDDVRGYKAKSSGGRDQLRWVL+VGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL
Sbjct: 121 DEEEDDVRGYKAKSSGGRDQLRWVLKVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL 180

Query: 181 KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI 240
           KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI
Sbjct: 181 KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI 240

Query: 241 EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI 300
           EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI
Sbjct: 241 EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI 300

Query: 301 HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKI 360
           HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLD+ETGKI
Sbjct: 301 HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDLETGKI 360

Query: 361 VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGA 420
           VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATF+GLDDNKLCQWDMRDRKGKVQNIGGA
Sbjct: 361 VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFMGLDDNKLCQWDMRDRKGKVQNIGGA 420

Query: 421 DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGS 480
           DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK SMRQAKTAFPGLGS
Sbjct: 421 DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKTSMRQAKTAFPGLGS 480

Query: 481 SINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLD 540
           SI HVDVTYDGKWILGTTDTYLILICTLFTDKDGN+KTGFSGRMGNRIPAPRLLKLTPLD
Sbjct: 481 SITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNIKTGFSGRMGNRIPAPRLLKLTPLD 540

Query: 541 SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKS 600
           SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDF QVKNSAHECYRNQQGLKS
Sbjct: 541 SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFQQVKNSAHECYRNQQGLKS 600

Query: 601 CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMK+SSISLSGKR
Sbjct: 601 CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKISSISLSGKR 650

BLAST of Cp4.1LG01g02260 vs. NCBI nr
Match: XP_022942384.1 (protein CYPRO4 [Cucurbita moschata])

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 635/648 (97.99%), Postives = 638/648 (98.46%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA 60
           MGTAHSRDCEDVELSDSDE KEEEEEEEEE CDEAGTELKPAKS GTSSAIDDVDAKLKA
Sbjct: 1   MGTAHSRDCEDVELSDSDEFKEEEEEEEEE-CDEAGTELKPAKSTGTSSAIDDVDAKLKA 60

Query: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE 120
           LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLT YDFLKTA VDGNNYD+E
Sbjct: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYDFLKTAKVDGNNYDEE 120

Query: 121 EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180
           EEDDVRGYKAKSSGGRDQLRWVL+VG KIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF
Sbjct: 121 EEDDVRGYKAKSSGGRDQLRWVLKVGTKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180

Query: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240
            SDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF
Sbjct: 181 RSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240

Query: 241 GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300
           GKSP SKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG
Sbjct: 241 GKSPGSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300

Query: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360
           KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT
Sbjct: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360

Query: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420
           EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMR+RKGKVQNIGGADN
Sbjct: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRERKGKVQNIGGADN 420

Query: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI 480
           SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK SMRQAKTAFPGLGSSI
Sbjct: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKTSMRQAKTAFPGLGSSI 480

Query: 481 NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540
            HVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH
Sbjct: 481 THVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540

Query: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600
           LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY
Sbjct: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600

Query: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 647

BLAST of Cp4.1LG01g02260 vs. NCBI nr
Match: KAG7030988.1 (Protein CYPRO4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1261 bits (3262), Expect = 0.0
Identity = 637/676 (94.23%), Postives = 639/676 (94.53%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA 60
           MGTAHSRDCEDVELSDSDE KEEEEEEEEE CDEAGTELKPAKS GTSSAIDDVDAKLKA
Sbjct: 1   MGTAHSRDCEDVELSDSDEFKEEEEEEEEE-CDEAGTELKPAKSTGTSSAIDDVDAKLKA 60

Query: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE 120
           LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLT YDFLKTA VDGNNYD+E
Sbjct: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYDFLKTAKVDGNNYDEE 120

Query: 121 EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180
           EEDDVRGYKAKSSGGRDQLRWVL+VGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF
Sbjct: 121 EEDDVRGYKAKSSGGRDQLRWVLKVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180

Query: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240
           PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF
Sbjct: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240

Query: 241 GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300
           GKSP SKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG
Sbjct: 241 GKSPGSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300

Query: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360
           KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT
Sbjct: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360

Query: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420
           EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN
Sbjct: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420

Query: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI 480
           SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK SMRQAKTAFPGLGSSI
Sbjct: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKTSMRQAKTAFPGLGSSI 480

Query: 481 NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540
            HVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH
Sbjct: 481 THVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540

Query: 541 LAGADNKFHGGHFSW----------------------------VTESGKQERYLVATVGK 600
           LAGADNKFHGGHFSW                            VTESGKQERYLVATVGK
Sbjct: 541 LAGADNKFHGGHFSWLAWLVGFCCLSPQVPYMTLQSYIDNDFQVTESGKQERYLVATVGK 600

Query: 601 FSVIWDFHQVKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLV 648
           FSVIWDFHQVKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEA LV
Sbjct: 601 FSVIWDFHQVKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEATLV 660

BLAST of Cp4.1LG01g02260 vs. NCBI nr
Match: XP_038895945.1 (protein CYPRO4 [Benincasa hispida])

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 585/659 (88.77%), Postives = 607/659 (92.11%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEE----EEEECDEAGTELKP------AKSAGTSSA 60
           MGTA SR  ED +LSDSDE +EEEEEE    EEEE  +A  ELKP      AKS GTSSA
Sbjct: 1   MGTAQSR--EDAKLSDSDEYEEEEEEEVEEEEEEEYKDADKELKPQSISTTAKSTGTSSA 60

Query: 61  IDDVDAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTA 120
           IDDVDAKLKAL LKYGSSSPSQ PN KNAVKLYLHIGGNTPRAKWIVSEKLTSY FLKTA
Sbjct: 61  IDDVDAKLKALKLKYGSSSPSQTPNFKNAVKLYLHIGGNTPRAKWIVSEKLTSYAFLKTA 120

Query: 121 NVDGNNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDF 180
           NVDG N DDEEED+  GYKA SS GR QLRWVL+VGAK+RALVSTEMQ+KMFGDQRRVDF
Sbjct: 121 NVDGGNDDDEEEDNDEGYKANSSAGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDF 180

Query: 181 VDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADD 240
           V+KGVWALKFP+DE+YRNFVTEFQDCLFENVYGLQATDEN+VKIYGKEFIGWLKPEVADD
Sbjct: 181 VNKGVWALKFPTDERYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPEVADD 240

Query: 241 SIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQV 300
           SIWENADIEFGKSP S   SVRTK DLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQV
Sbjct: 241 SIWENADIEFGKSPSS---SVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQV 300

Query: 301 YKNLSHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQ 360
           Y+NLSHGIHG GVSLK G+G+SPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGL+Q
Sbjct: 301 YRNLSHGIHGTGVSLKFGAGHSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLEQ 360

Query: 361 LDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKG 420
           LDIETGKIVTEWKFEKDG DITMRDITN+TKGSQLDPSE+TFLGLDDN+LCQWDMRDR+G
Sbjct: 361 LDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRG 420

Query: 421 KVQNIGG-ADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQA 480
            VQNIGG ADNS VLNWAQGHQF RGTNFQCFATTGDGSIVVGS DGKIRLYSK SMRQA
Sbjct: 421 MVQNIGGPADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQA 480

Query: 481 KTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAP 540
           KTAFPGLGS I HVDVTYDGKWILGT D+YLILICTLFTDKDGN KTGFSGRMGNRIPAP
Sbjct: 481 KTAFPGLGSPITHVDVTYDGKWILGTADSYLILICTLFTDKDGNTKTGFSGRMGNRIPAP 540

Query: 541 RLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHEC 600
           RLLKLTPLDSHLAG DNKFHGGHFSWVTESGKQER+LVATVGKFSVIWDFH VKNSAH+C
Sbjct: 541 RLLKLTPLDSHLAGTDNKFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHHVKNSAHDC 600

Query: 601 YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAV DSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 654

BLAST of Cp4.1LG01g02260 vs. ExPASy TrEMBL
Match: A0A6J1IR30 (protein CYPRO4 OS=Cucurbita maxima OX=3661 GN=LOC111479222 PE=4 SV=1)

HSP 1 Score: 1273 bits (3295), Expect = 0.0
Identity = 635/650 (97.69%), Postives = 640/650 (98.46%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEE--CDEAGTELKPAKSAGTSSAIDDVDAKL 60
           MGTAHSRDCEDVELSDSDE KEEEEEEEEEE  CDEAGTELK  KS GTSSAIDDVDAKL
Sbjct: 1   MGTAHSRDCEDVELSDSDEFKEEEEEEEEEEEECDEAGTELKLDKSTGTSSAIDDVDAKL 60

Query: 61  KALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD 120
           KALNLKYGSSSP QIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD
Sbjct: 61  KALNLKYGSSSPYQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYD 120

Query: 121 DEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL 180
           DEEEDDVRGYKAKSSGGRDQLRWVL+VGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL
Sbjct: 121 DEEEDDVRGYKAKSSGGRDQLRWVLKVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWAL 180

Query: 181 KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI 240
           KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI
Sbjct: 181 KFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADI 240

Query: 241 EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI 300
           EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI
Sbjct: 241 EFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGI 300

Query: 301 HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKI 360
           HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLD+ETGKI
Sbjct: 301 HGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDLETGKI 360

Query: 361 VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGA 420
           VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATF+GLDDNKLCQWDMRDRKGKVQNIGGA
Sbjct: 361 VTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFMGLDDNKLCQWDMRDRKGKVQNIGGA 420

Query: 421 DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGS 480
           DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK SMRQAKTAFPGLGS
Sbjct: 421 DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKTSMRQAKTAFPGLGS 480

Query: 481 SINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLD 540
           SI HVDVTYDGKWILGTTDTYLILICTLFTDKDGN+KTGFSGRMGNRIPAPRLLKLTPLD
Sbjct: 481 SITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNIKTGFSGRMGNRIPAPRLLKLTPLD 540

Query: 541 SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKS 600
           SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDF QVKNSAHECYRNQQGLKS
Sbjct: 541 SHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFQQVKNSAHECYRNQQGLKS 600

Query: 601 CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMK+SSISLSGKR
Sbjct: 601 CYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKISSISLSGKR 650

BLAST of Cp4.1LG01g02260 vs. ExPASy TrEMBL
Match: A0A6J1FR52 (protein CYPRO4 OS=Cucurbita moschata OX=3662 GN=LOC111447445 PE=4 SV=1)

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 635/648 (97.99%), Postives = 638/648 (98.46%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTELKPAKSAGTSSAIDDVDAKLKA 60
           MGTAHSRDCEDVELSDSDE KEEEEEEEEE CDEAGTELKPAKS GTSSAIDDVDAKLKA
Sbjct: 1   MGTAHSRDCEDVELSDSDEFKEEEEEEEEE-CDEAGTELKPAKSTGTSSAIDDVDAKLKA 60

Query: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDGNNYDDE 120
           LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLT YDFLKTA VDGNNYD+E
Sbjct: 61  LNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYDFLKTAKVDGNNYDEE 120

Query: 121 EEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180
           EEDDVRGYKAKSSGGRDQLRWVL+VG KIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF
Sbjct: 121 EEDDVRGYKAKSSGGRDQLRWVLKVGTKIRALVSTEMQMKMFGDQRRVDFVDKGVWALKF 180

Query: 181 PSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240
            SDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF
Sbjct: 181 RSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWENADIEF 240

Query: 241 GKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300
           GKSP SKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG
Sbjct: 241 GKSPGSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHG 300

Query: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360
           KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT
Sbjct: 301 KGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVT 360

Query: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQNIGGADN 420
           EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMR+RKGKVQNIGGADN
Sbjct: 361 EWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRERKGKVQNIGGADN 420

Query: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAFPGLGSSI 480
           SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK SMRQAKTAFPGLGSSI
Sbjct: 421 SPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKTSMRQAKTAFPGLGSSI 480

Query: 481 NHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540
            HVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH
Sbjct: 481 THVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLKLTPLDSH 540

Query: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600
           LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY
Sbjct: 541 LAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQQGLKSCY 600

Query: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 CYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 647

BLAST of Cp4.1LG01g02260 vs. ExPASy TrEMBL
Match: A0A6J1JB96 (protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1)

HSP 1 Score: 1129 bits (2920), Expect = 0.0
Identity = 572/655 (87.33%), Postives = 606/655 (92.52%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEE-EEEECDEAGTELKP-----AKSAGTSSAIDDV 60
           MGTA SR  ED ELSDSDE KEEEEE+ EEEE ++A   LKP     AKS GT+SA+DDV
Sbjct: 1   MGTAQSR--EDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDV 60

Query: 61  DAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDG 120
           DAKLKAL LKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLT Y FLKTANVDG
Sbjct: 61  DAKLKALKLKYGSSSPSQIPNASNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDG 120

Query: 121 NNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKG 180
           ++ DD++EDD   YKA SSGGR QLRW+L+VGAK+RALVSTEMQ+KMFGDQRRVDFV+KG
Sbjct: 121 DS-DDDQEDDYDAYKANSSGGRGQLRWILKVGAKVRALVSTEMQLKMFGDQRRVDFVNKG 180

Query: 181 VWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWE 240
           VWALKFPSDEQYR+FVT+FQD LFENVYGL+AT+EN+VKIYGKEFIGWLKPEVADDSIWE
Sbjct: 181 VWALKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWE 240

Query: 241 NADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNL 300
           NADIEFGKSP S   SVRTK DLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVY+NL
Sbjct: 241 NADIEFGKSPSS---SVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNL 300

Query: 301 SHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIE 360
           SHGIHGKG+SLK G+GNSPN  RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIE
Sbjct: 301 SHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIE 360

Query: 361 TGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQN 420
           TGK+VTEWKFEKDG DITMRDITN+TKGSQLDPSE+TFLGLDDN+LCQWDMRDR+G VQN
Sbjct: 361 TGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQN 420

Query: 421 IGGA-DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAF 480
           IGGA DNSPVLNWAQGHQF RGTNFQCFATTGDGSIVVGS DGKIRLYSK SMRQAKTAF
Sbjct: 421 IGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAF 480

Query: 481 PGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLK 540
           PGLGS I HVDVT+DGKWILGTTDTYLILIC LF DKDGN KTGFSGRMGN+IPAPRLLK
Sbjct: 481 PGLGSPITHVDVTHDGKWILGTTDTYLILICVLFIDKDGNTKTGFSGRMGNKIPAPRLLK 540

Query: 541 LTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQ 600
           LTPLDSHLAG DN+FHGGHFSWVTESGKQE +LVATVGKFSVIWDFHQVKNSAH+CYRNQ
Sbjct: 541 LTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQ 600

Query: 601 QGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           QGLKSCYCYKIVLKDESIVESRFMH+KFAV DSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 QGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Cp4.1LG01g02260 vs. ExPASy TrEMBL
Match: A0A6J1E9T2 (protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1)

HSP 1 Score: 1123 bits (2905), Expect = 0.0
Identity = 568/655 (86.72%), Postives = 605/655 (92.37%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEE-EEEECDEAGTELKP-----AKSAGTSSAIDDV 60
           MGTA SR  ED ELSDSDE KEEEEE+ EEEE ++A   LKP     AKS GT+SA+DDV
Sbjct: 1   MGTAQSR--EDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDV 60

Query: 61  DAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKTANVDG 120
           DAKLKAL LKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLT Y FLKT+NVDG
Sbjct: 61  DAKLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDG 120

Query: 121 NNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVDFVDKG 180
           ++ DD++ED+  GYKA SSGGR QLRW+L+VG K+RALVSTEMQ+KMFGDQRRVDFV+KG
Sbjct: 121 DS-DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKG 180

Query: 181 VWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVADDSIWE 240
           VWALKFP+DEQYR+FVT+FQD LFENVYGL+AT+EN+VKIYGKEFIGWLKPEVADDSIWE
Sbjct: 181 VWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWE 240

Query: 241 NADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYKNL 300
           NADIEFGKSP S   SVRTK DLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVY+NL
Sbjct: 241 NADIEFGKSPGS---SVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNL 300

Query: 301 SHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIE 360
           SHGIHGKG+SLK G+GNSPN  RSTPKKGLL KAETNMLLMSPLKEGKPHASGLQQLDIE
Sbjct: 301 SHGIHGKGISLKFGAGNSPNSSRSTPKKGLLTKAETNMLLMSPLKEGKPHASGLQQLDIE 360

Query: 361 TGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMRDRKGKVQN 420
           TGK+VTEWKFEKDG DITMRDITN+TKGSQLDPSE+TFLGLDDN+LCQWDMRDR+G VQN
Sbjct: 361 TGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQN 420

Query: 421 IGGA-DNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASMRQAKTAF 480
           IGGA DNSPVLNWAQGHQF RGTNFQCFATTGDGSIVVGS DGKIRLYSK SMRQAKTAF
Sbjct: 421 IGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAF 480

Query: 481 PGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRIPAPRLLK 540
           PGLGS I HVDVT+DGKWILGTTDTYLILIC LF DKDGN KTGFSGRMGN+IPAPRLLK
Sbjct: 481 PGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLK 540

Query: 541 LTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSAHECYRNQ 600
           LTPLDSHLAG DN+FHGGHFSWVTESGKQE +LVATVGKFSVIWDFHQVKNSAH+CYRNQ
Sbjct: 541 LTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQ 600

Query: 601 QGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSISLSGKR 648
           QGLKSCYCYKIVLKDESIVESRFMH+KFAV DSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 QGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Cp4.1LG01g02260 vs. ExPASy TrEMBL
Match: A0A5D3DQK8 (Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G00020 PE=4 SV=1)

HSP 1 Score: 1118 bits (2893), Expect = 0.0
Identity = 574/665 (86.32%), Postives = 604/665 (90.83%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEE----EEECDEAGTELKP------AKSAGTSSA 60
           MG+  SR  EDVELSDSD+ KEEEE+++    EEE  +A  ELKP      AKS GTSSA
Sbjct: 1   MGSGQSR--EDVELSDSDDYKEEEEDDDDDDDEEEYQDADKELKPHSVSTTAKSTGTSSA 60

Query: 61  IDDVDAKLKALNLKYGSSS------PSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSY 120
           IDDVDAKLKAL LKYGSSS      PS+ PNSKNAVKLYLHIGGNTPRAKWIVSEKL+ Y
Sbjct: 61  IDDVDAKLKALKLKYGSSSSSPSPSPSKTPNSKNAVKLYLHIGGNTPRAKWIVSEKLSFY 120

Query: 121 DFLKTANVDGNNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGD 180
            FLKTANVDG+N DDEEEDD +GY A SSGGR   RWVL+VGAK+RALVSTEMQ+KMFGD
Sbjct: 121 AFLKTANVDGHNDDDEEEDDDQGYNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGD 180

Query: 181 QRRVDFVDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLK 240
           QRRVDFV+KGVWALKFPS EQYRNFVTEFQDCLFENVYGLQATDEN+VKIYGKEFIGWLK
Sbjct: 181 QRRVDFVNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLK 240

Query: 241 PEVADDSIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVN 300
           PEVADDSIWENADIEF KSP S   SVRTK DLIEEFEEAANGGVQSLTLGALDNSFLVN
Sbjct: 241 PEVADDSIWENADIEFEKSPSS---SVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVN 300

Query: 301 DSGVQVYKNLSHGIHGKGVSLKVGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPH 360
           DSGVQVY+NLSHGIHGKGVSLK G+G+SPN+ RSTPKKGLLMKAETNMLLMSPLKEGKPH
Sbjct: 301 DSGVQVYRNLSHGIHGKGVSLKFGAGHSPNVWRSTPKKGLLMKAETNMLLMSPLKEGKPH 360

Query: 361 ASGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWD 420
            +GL+QLDIETGKIVTEWKFEKDG DITMRDITN+TKGSQLDPSE+TFLGLDDN+LCQWD
Sbjct: 361 TTGLEQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWD 420

Query: 421 MRDRKGKVQNIGG-ADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSK 480
           MRDR+G VQNI G ADNS VLNWAQGHQF RGTNFQCFATTGDGSIVVGS DGKIRLYSK
Sbjct: 421 MRDRRGMVQNISGSADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSK 480

Query: 481 ASMRQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMG 540
            SMRQAKTAFPGLGS I HVDVTYDGKWILGTTD+YLILICTLFTDKDGN KTGFSGRMG
Sbjct: 481 TSMRQAKTAFPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMG 540

Query: 541 NRIPAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVK 600
           NRIPAPRLLKLTPLDSHLAG DN FHGGHFSWVTESGKQER+LVATVGKFSVIWDFH+VK
Sbjct: 541 NRIPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVK 600

Query: 601 NSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSSIS 648
           NS+H+CYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAV DSPEAPLVVATPMKVSSIS
Sbjct: 601 NSSHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSIS 657

BLAST of Cp4.1LG01g02260 vs. TAIR 10
Match: AT3G19240.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 896.0 bits (2314), Expect = 1.8e-260
Identity = 457/664 (68.83%), Postives = 534/664 (80.42%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEEEEEEEEECDEAGTE-------LKPAKSAGTSSAID- 60
           MGT+ SR+ + +  SD++   +  EEE+E++ D+A  +        +P+ S+ +S   D 
Sbjct: 1   MGTSQSRE-DRISESDTESDSDYYEEEDEDQYDDAKKQGSSSVSGTRPSDSSSSSGPTDS 60

Query: 61  ---DVDAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDFLKT 120
              D++ KLKAL LKY SSS S  P  KNAVKLY HIGGNTP+AKWIVS+K+TSY F+KT
Sbjct: 61  SSLDIEQKLKALKLKYPSSS-SVTPKMKNAVKLYRHIGGNTPKAKWIVSDKMTSYKFVKT 120

Query: 121 ANVDGNNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQRRVD 180
           ++VDG + DD ++        + SG   +  W L VG K++A VST+MQ+KMFGDQRRVD
Sbjct: 121 SSVDGEDIDDYDD-------CEESGEGGESFWFLGVGTKVKARVSTDMQLKMFGDQRRVD 180

Query: 181 FVDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPEVAD 240
           FV  GVWALKF +DE YR FVT FQD LFENVY ++A++ENRVK+YGK+FIGW  PE AD
Sbjct: 181 FVSNGVWALKFLTDEDYRKFVTRFQDYLFENVYKIRASEENRVKVYGKDFIGWANPEAAD 240

Query: 241 DSIWENADIEFGKSPRSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ 300
           DS+WE+A+      P  +    +   DL EEFEE ANGGVQSLTLGALDNSFLVND GVQ
Sbjct: 241 DSMWEDAE----APPEEEETQGKRNTDLTEEFEEVANGGVQSLTLGALDNSFLVNDYGVQ 300

Query: 301 VYKNLSHGIHGKGVSLKVGSGNS---PNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHAS 360
           VY+N+  GIHGKGV ++  SGNS       ++TP K LLM+AETNM+LMSP K+GKP+++
Sbjct: 301 VYRNMERGIHGKGVCVRFDSGNSKFGSGSSQTTPNKALLMRAETNMMLMSPAKQGKPNST 360

Query: 361 GLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLCQWDMR 420
           G++QLDIE+GKIVTEWKFEKDG +ITMRDITN+TKGSQLDPSE+TFLGLDDN+LCQWDMR
Sbjct: 361 GVKQLDIESGKIVTEWKFEKDGTEITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMR 420

Query: 421 DRKGKVQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLYSKASM 480
           DR+G VQNI    +SP+L W QGHQF RGTNFQCFATTGDGSIVVGS DGKIRLYSK SM
Sbjct: 421 DRRGIVQNI----DSPILEWTQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLYSKTSM 480

Query: 481 RQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGRMGNRI 540
           R AKTAFPGLGS I HVDV+YDGKWILGTTDTYL+LICTLFTDK+G  KTGFSGRMGN+I
Sbjct: 481 RMAKTAFPGLGSPITHVDVSYDGKWILGTTDTYLVLICTLFTDKNGLTKTGFSGRMGNKI 540

Query: 541 PAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQVKNSA 600
           PAPRLLKLTPLDSHLAG DNKFHGGHFSWVTESGKQER++VATVGKFSVIWD  +VKNSA
Sbjct: 541 PAPRLLKLTPLDSHLAGKDNKFHGGHFSWVTESGKQERHIVATVGKFSVIWDLERVKNSA 600

Query: 601 HECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVL--DSPEAPLVVATPMKVSSISL 649
           HECYRNQQGLKSCYCYKI+LKDESIVESRFMHD F+     SPEAPLVVATP+KVSSISL
Sbjct: 601 HECYRNQQGLKSCYCYKILLKDESIVESRFMHDNFSFSGNKSPEAPLVVATPLKVSSISL 647

BLAST of Cp4.1LG01g02260 vs. TAIR 10
Match: AT4G33400.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 785.4 bits (2027), Expect = 3.5e-227
Identity = 408/667 (61.17%), Postives = 499/667 (74.81%), Query Frame = 0

Query: 1   MGTAHSRDCEDVELSDSDESKEEE-------EEEEEEECDEAGTELKPAKSAG------- 60
           MG +HS   ED+E+  SDE + EE       EEEEE   D     L  + S G       
Sbjct: 1   MGASHSH--EDLEICSSDEDEYEEYEEGREGEEEEETFQDSRDDTLGISSSGGRRLRPKS 60

Query: 61  TSSAIDDVDAKLKALNLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTSYDF 120
            SS++DDV+AKL+AL LKY  +  +  P+++N+ +L+ +I GNTP+AKW+ +EKLT+Y F
Sbjct: 61  PSSSLDDVEAKLQALKLKYPLTQSA--PSTQNSARLFRYINGNTPKAKWVTAEKLTAYCF 120

Query: 121 LKTANVDGNNYDDEEEDDVRGYKAKSSGGRDQLRWVLQVGAKIRALVSTEMQMKMFGDQR 180
           +KT   D ++ DD+E  DV           +   W+L+VG+KIR  VS EMQ+K + DQR
Sbjct: 121 VKTNKGDEDDEDDDENGDV-----------ENEWWILKVGSKIREKVSDEMQLKAYKDQR 180

Query: 181 RVDFVDKGVWALKFPSDEQYRNFVTEFQDCLFENVYGLQATDENRVKIYGKEFIGWLKPE 240
           RVDFV K VWA+KF S E +  FV+ + +CLFEN +G++  + N+ KIYGK+FIGW  PE
Sbjct: 181 RVDFVAKAVWAMKFASSEDFSVFVSSYNNCLFENNHGVEFNEANKAKIYGKDFIGWANPE 240

Query: 241 VADDSIWENADIEFGKSP--RSKTPSVRTKHDLIEEFEEAANGGVQSLTLGALDNSFLVN 300
            ADDS+WE+AD    +SP   S TP+ R   DL E FEEA + G+ SL LGALDNSFLV 
Sbjct: 241 AADDSMWEDADDILLQSPGGGSATPA-RDTQDLTEAFEEATSEGIHSLALGALDNSFLVG 300

Query: 301 DSGVQVYKNLSHGIHGKGVSLKVGSGNSPNIGR---STPKKGLLMKAETNMLLMSPLKEG 360
           DSG+QV+KN+  GI GKGV +       P  GR   S PKK LLM+AETNMLLMSP+ + 
Sbjct: 301 DSGIQVFKNMRQGIQGKGVCVNF----EPGYGRTHSSAPKKALLMRAETNMLLMSPMSQ- 360

Query: 361 KPHASGLQQLDIETGKIVTEWKFEKDGNDITMRDITNNTKGSQLDPSEATFLGLDDNKLC 420
            PH+ G+ QLDIETGKI++EWKFEKDG DI+M DITN+ KG+QLDPS +TFLGLD+N+LC
Sbjct: 361 TPHSRGIHQLDIETGKIISEWKFEKDGVDISMSDITNDGKGAQLDPSASTFLGLDNNRLC 420

Query: 421 QWDMRDRKGKVQNIGGADNSPVLNWAQGHQFCRGTNFQCFATTGDGSIVVGSADGKIRLY 480
           +WDMRDR G VQ++  A N+PVLNWAQGHQF RGTNFQCFATTGDGSIVVGS DGKIRLY
Sbjct: 421 RWDMRDRYGMVQDLATA-NTPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLY 480

Query: 481 SKASMRQAKTAFPGLGSSINHVDVTYDGKWILGTTDTYLILICTLFTDKDGNMKTGFSGR 540
           S  +MRQAKTAFPGLG+ + HVD T+DGKWI+GTTDTYLI+ICTLFTDK G  KTGF GR
Sbjct: 481 SSNTMRQAKTAFPGLGAPVTHVDATFDGKWIVGTTDTYLIVICTLFTDKSGKTKTGFEGR 540

Query: 541 MGNRIPAPRLLKLTPLDSHLAGADNKFHGGHFSWVTESGKQERYLVATVGKFSVIWDFHQ 600
           MGN+I APRLLKL PLD+HLAG+DNKF    FSWVTE GKQER++VATVGKFSVIW+F Q
Sbjct: 541 MGNKIAAPRLLKLRPLDAHLAGSDNKFRNAQFSWVTEDGKQERHVVATVGKFSVIWNFQQ 600

Query: 601 VKNSAHECYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVLDSPEAPLVVATPMKVSS 649
           VKN +HECY +Q+GLK CYCYKIVL++ESIV+SRFM+D FA+  SPEAPLV+ATPMKVSS
Sbjct: 601 VKNGSHECYHDQEGLKKCYCYKIVLRNESIVDSRFMNDNFAISGSPEAPLVIATPMKVSS 645

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P407812.1e-22476.72Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1[more]
Q555V75.0e-2925.84VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=... [more]
Q1MTR38.8e-2629.57Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain ... [more]
P401571.0e-1826.27Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain A... [more]
Match NameE-valueIdentityDescription
XP_023529490.10.0100.00protein CYPRO4-like [Cucurbita pepo subsp. pepo][more]
XP_022979546.10.097.69protein CYPRO4 [Cucurbita maxima][more]
XP_022942384.10.097.99protein CYPRO4 [Cucurbita moschata][more]
KAG7030988.10.094.23Protein CYPRO4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038895945.10.088.77protein CYPRO4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1IR300.097.69protein CYPRO4 OS=Cucurbita maxima OX=3661 GN=LOC111479222 PE=4 SV=1[more]
A0A6J1FR520.097.99protein CYPRO4 OS=Cucurbita moschata OX=3662 GN=LOC111447445 PE=4 SV=1[more]
A0A6J1JB960.087.33protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1[more]
A0A6J1E9T20.086.72protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1[more]
A0A5D3DQK80.086.32Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G0002... [more]
Match NameE-valueIdentityDescription
AT3G19240.11.8e-26068.83Vacuolar import/degradation, Vid27-related protein [more]
AT4G33400.13.5e-22761.17Vacuolar import/degradation, Vid27-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 18..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..35
NoneNo IPR availablePANTHERPTHR31913:SF7DEM PROTEINcoord: 1..648
NoneNo IPR availableSUPERFAMILY101908Putative isomerase YbhEcoord: 348..547
IPR013863Vacuolar import/degradation Vid27, C-terminalPFAMPF08553VID27coord: 277..591
e-value: 3.5E-42
score: 144.7
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 333..558
e-value: 1.4E-8
score: 36.4
IPR040458Vacuolar import/degradation Vid27PANTHERPTHR31913VACUOLAR IMPORT AND DEGRADATION PROTEIN 27coord: 1..648

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g02260.1Cp4.1LG01g02260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding