Cp4.1LG01g02050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g02050
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	6-phosphogluconate dehydrogenase family protein
Location	Cp4.1LG01: 2691616 .. 2693030 (-)
RNA-Seq Expression	Cp4.1LG01g02050
Synteny	Cp4.1LG01g02050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAATCAAGAACTTGTGGGGCGAACTTGTGAAGAAAAGATGGATCGCTATGTCTGCTAATTATCTGTAAGTTTGGTTTTGAAAATTCCTTATTTTCCACCATGGAAACTCCTTACCCAATCCCCATCTCTCCCACGAAAACTCGCATTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGTGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTGAATCCTGGTGGAGTCATCGTTGACACCACCAGCAGCCACCCAGCTCTTGCCCGCGAGATATTTGCTGCTGCTCGTGCTAAGGGTCGTGTTAAGTTCCACTAAATTACTAGATCAAATCGAACCACCTTGAACTAAGAAGTTTAAGCTTGTTTACTCGTATTCTTGGCAGGTTGCTGGGCCGTGGATGCGCCTGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGCTTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCCAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCCGTTGTTCAGAGGCTTAATGGCAAGTAAGTGAATCTGTAGGAAACTTGTTTGTTTATTGCTTGTTTGTGCACTTGGGATTGGAAGCCAATTCTCTTGTACTGATAGTTTATAGAAGTTGTTTTCGAGTAAGTCCCATTTTAAGATTTAGAATTTGAATTCGACACTTAATGACTCTTGTACTCTCCTTTCTTTCCTGTGACATGACTACGTTATCTATTTTAGAGTTAAGAAAATCCTCGTAGACCAACTAGACTTGATTTATTTCCTGTGACATGACTACGTTATCTATTTTAGAGTTAAGAAAATCCTCATAGACCAACCAGACTTGA

mRNA sequence

AACAAATCAAGAACTTGTGGGGCGAACTTGTGAAGAAAAGATGGATCGCTATGTCTGCTAATTATCTGTAAGTTTGGTTTTGAAAATTCCTTATTTTCCACCATGGAAACTCCTTACCCAATCCCCATCTCTCCCACGAAAACTCGCATTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGTGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTGAATCCTGGTGGAGTCATCGTTGACACCACCAGCAGCCACCCAGCTCTTGCCCGCGAGATATTTGCTGCTGCTCGTGCTAAGGGTTGCTGGGCCGTGGATGCGCCTGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGCTTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCCAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCCGTTGTTCAGAGGCTTAATGGCAAGTAAGTGAATCTGTAGGAAACTTGTTTGTTTATTGCTTGTTTGTGCACTTGGGATTGGAAGCCAATTCTCTTGTACTGATAGTTTATAGAAGTTGTTTTCGAGTAAGTCCCATTTTAAGATTTAGAATTTGAATTCGACACTTAATGACTCTTGTACTCTCCTTTCTTTCCTGTGACATGACTACGTTATCTATTTTAGAGTTAAGAAAATCCTCGTAGACCAACTAGACTTGATTTATTTCCTGTGACATGACTACGTTATCTATTTTAGAGTTAAGAAAATCCTCATAGACCAACCAGACTTGA

Coding sequence (CDS)

ATGGAAACTCCTTACCCAATCCCCATCTCTCCCACGAAAACTCGCATTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGTGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTGAATCCTGGTGGAGTCATCGTTGACACCACCAGCAGCCACCCAGCTCTTGCCCGCGAGATATTTGCTGCTGCTCGTGCTAAGGGTTGCTGGGCCGTGGATGCGCCTGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGCTTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCCAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCCGTTGTTCAGAGGCTTAATGGCAAGTAA

Protein sequence

METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLNGK

Homology

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.1e-103
Identity = 189/306 (61.76%), Postives = 236/306 (77.12%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I P+KTRIGWIGIG+MG+AM + ++AAGYSV +YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS  ELA++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+ +V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQIA   NL+GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM  +             +PGAAL+KQLF+ MVANGDGK G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 322 IRRLNG 316

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 9.8e-100
Identity = 182/306 (59.48%), Postives = 229/306 (74.84%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           MET YP  I P+KTRIGWIGIG+MG+AM + +LAAGYSV +YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS  EL ++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD+ +V+WL+P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQI +G N++GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM           E  + +PG AL KQLF+ MVANGDGK G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMA---------AEAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 301 IRRLNG 297

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.3e-79
Identity = 150/299 (50.17%), Postives = 220/299 (73.58%), Query Frame = 0


Query: 9   ISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQ 68
           I+P+ T+IGWIG GVMG +M   L+ AGY+V ++ R+ SKA  L   GA++ +S + +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  VSDIVFTMVGHPSDVRQNVLE-KNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCW 128
            SD+VFT+VG+PSDVR  +L+ K+G L GL  GGV+VD T+S P+LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++GKL+IF GGD   VK L PLF  +GKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEY 248
            I   ++GL EGL++A +AGLDVK+F+EA+  GAAGS +++L+G+R+++RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGEDVERL-VVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLN 306
            VKDLG+ ++      + +R+ + LPG ALA+QL+ ++ A+G+G  GTQ L+  ++RLN
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLN 325

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.6e-54
Identity = 109/287 (37.98%), Postives = 184/287 (64.11%), Query Frame = 0


Query: 13  KTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDI 72
           K  IG+IG+GVMG +MA+ +L  G+ V +Y R+  KA  +  KGA   ++  +L++ +D+
Sbjct: 2   KKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADV 61

Query: 73  VFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCWAVDAP 132
           + TMVG+PSDV +     NG+++    G  ++D T+S P+LA++I  AA+ K  +A+DAP
Sbjct: 62  IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAP 121

Query: 133 VSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGK-VTYVGEAGCGQSCKIANQIAIGG 192
           VSGGDIGA++G LAI  GG+    +   P+F  +G+ + Y G AG GQ  K+ NQIAI  
Sbjct: 122 VSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAA 181

Query: 193 NLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKD 252
            ++G++E + +A+++GL+ +  ++++  GAAGS ++     RM++ +F PG + ++ +KD
Sbjct: 182 GMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKD 241

Query: 253 LGMGVDVVE-EGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGL 298
           +G+ ++  E  GE+      +PG +LAK L+  + A G+   GTQ +
Sbjct: 242 MGIALEEAELMGEE------MPGLSLAKSLYDKLAAQGEENSGTQSI 282

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot
Match: P77161 (2-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN=glxR PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.9e-42
Identity = 109/290 (37.59%), Postives = 161/290 (55.52%), Query Frame = 0


Query: 15  RIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVF 74
           ++G+IG+G+MG  MA  L  AG+ + +    P  A +L S GA  V +A ++ + SDI+F
Sbjct: 2   KLGFIGLGIMGTPMAINLARAGHQLHVTTIGP-VADELLSLGAVSVETARQVTEASDIIF 61

Query: 75  TMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCWAVDAPVS 134
            MV     V + +  +NG  +    G  IVD +S  P   +         G   +DAPVS
Sbjct: 62  IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS 121

Query: 135 GGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGK-VTYVGEAGCGQSCKIANQIAIGGNL 194
           GG+IGAR+G L+I  GGD  V + + PLFE LGK +T VG  G GQ+CK+ANQI +  N+
Sbjct: 122 GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI 181

Query: 195 MGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLG 254
             +SE L+FA +AG D  +  +A+ GG A S  +E+ GERMI+R F PG       KDL 
Sbjct: 182 EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN 241

Query: 255 MGVDVVEEGEDVERLVV-LPGAALAKQLFSAMVANGDGKFGTQGLISVVQ 303
           + +      +  + L + LP  A  ++LF+   ANG  +     L+  ++
Sbjct: 242 LAL------QSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALE 284

[Close]

BLAST of Cp4.1LG01g02050 vs. NCBI nr
Match: XP_023533858.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1526), Expect = 1.03e-212
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. NCBI nr
Match: XP_023533851.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1526), Expect = 1.55e-212
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. NCBI nr
Match: KAG6600356.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590 bits (1520), Expect = 4.87e-212
Identity = 306/307 (99.67%), Postives = 306/307 (99.67%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHP LAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. NCBI nr
Match: KAG7031015.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587 bits (1514), Expect = 4.00e-211
Identity = 304/307 (99.02%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGA LV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGACLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQ SDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSASELAQTSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNG+
Sbjct: 301 VQRLNGR 307

[Close]

BLAST of Cp4.1LG01g02050 vs. NCBI nr
Match: XP_022942972.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 587 bits (1514), Expect = 6.98e-211
Identity = 304/307 (99.02%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKV YVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVAYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGL EGLVFAERAGLDVKQFVEA+RGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLGEGLVFAERAGLDVKQFVEALRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL
Match: A0A6J1FRQ4 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447848 PE=4 SV=1)

HSP 1 Score: 587 bits (1514), Expect = 3.38e-211
Identity = 304/307 (99.02%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKV YVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVAYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGL EGLVFAERAGLDVKQFVEA+RGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLGEGLVFAERAGLDVKQFVEALRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL
Match: A0A6J1J121 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111480910 PE=4 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 1.13e-209
Identity = 303/307 (98.70%), Postives = 304/307 (99.02%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPI I PTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHP LAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGEDVER+VVLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           VQRLNGK
Sbjct: 301 VQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL
Match: A0A6J1D789 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017673 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 6.55e-196
Identity = 279/307 (90.88%), Postives = 295/307 (96.09%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIP++PTKTRIGWIGIGVMGAAMATRLL+AGYSVAIYAR+PSKALDLQSKGA L+
Sbjct: 1   METPYPIPVTPTKTRIGWIGIGVMGAAMATRLLSAGYSVAIYARTPSKALDLQSKGARLL 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NSA E+A+ SD+VFTMVGHPSDVRQNVLE NGLLQGLNPGGV+VDTTSSHPALAREIFAA
Sbjct: 61  NSAVEVARTSDVVFTMVGHPSDVRQNVLENNGLLQGLNPGGVVVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAK CWAVDAPVSGGDIGARDGKLAIF GGDAGVVKWL+PLFE LGKVT+VGE+GCGQS
Sbjct: 121 ARAKDCWAVDAPVSGGDIGARDGKLAIFAGGDAGVVKWLAPLFEILGKVTHVGESGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI IG NLMGLSEGLVFAERAGLD+KQFVEAVRGGAAGSMAMELFGERMI RDFR
Sbjct: 181 CKIANQITIGANLMGLSEGLVFAERAGLDLKQFVEAVRGGAAGSMAMELFGERMIRRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGED ER+ VLPGAALAKQLFSAMVANGDGKFGTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGED-ERIAVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 VQRLNGK 307
           ++RLNGK
Sbjct: 301 IERLNGK 306

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL
Match: A0A6J1FSW9 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447849 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 1.86e-193
Identity = 276/307 (89.90%), Postives = 293/307 (95.44%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISP+KTR+GWIGIGVMGAAMATRLLAAGYS+ IYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS SELAQ  D+VFTMVG+PSDVRQNVL+KNGLLQGLNPGGVIVDTTSSHPALAREIFAA
Sbjct: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLQKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKL+IFGGGDA VVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI + GN++G+SEGLVFAE+AGLDVKQFVEAVRGGA GSMAMELFG RMIERDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAEQAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGG+AEYMVKDL M VDVVEEGEDVER+ VLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 VQRLNGK 307
           +QRLNGK
Sbjct: 301 IQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL
Match: A0A6J1J1I1 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111481012 PE=4 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.03e-190
Identity = 273/307 (88.93%), Postives = 290/307 (94.46%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYPIPISP+KTR+GWIGIGVMGAAMATRLLAAGYS+ IYARSPSKALDLQSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS SELA   D+VFTMVG+PSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREI AA
Sbjct: 61  NSPSELAHTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREISAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKL+IFGGGDA VVKWLSPLFEALGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI + GN++G+SEGLVFAERAGLDVKQFVEAVR GA GSMAMELFG RM++RDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAERAGLDVKQFVEAVRDGAGGSMAMELFGGRMVKRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGG+AEYMVKDL M VDVVEEGEDVER+ VLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 VQRLNGK 307
           +QRLNGK
Sbjct: 301 IQRLNGK 307

[Close]

BLAST of Cp4.1LG01g02050 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 377.9 bits (969), Expect = 7.9e-105
Identity = 189/306 (61.76%), Postives = 236/306 (77.12%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I P+KTRIGWIGIG+MG+AM + ++AAGYSV +YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS  ELA++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+ +V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQIA   NL+GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM  +             +PGAAL+KQLF+ MVANGDGK G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 322 IRRLNG 316

[Close]

BLAST of Cp4.1LG01g02050 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 364.8 bits (935), Expect = 6.9e-101
Identity = 182/306 (59.48%), Postives = 229/306 (74.84%), Query Frame = 0


Query: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           MET YP  I P+KTRIGWIGIG+MG+AM + +LAAGYSV +YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120
           NS  EL ++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD+ +V+WL+P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQI +G N++GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM           E  + +PG AL KQLF+ MVANGDGK G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMA---------AEAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 301 IRRLNG 297

[Close]

BLAST of Cp4.1LG01g02050 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.2e-80
Identity = 150/299 (50.17%), Postives = 220/299 (73.58%), Query Frame = 0


Query: 9   ISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQ 68
           I+P+ T+IGWIG GVMG +M   L+ AGY+V ++ R+ SKA  L   GA++ +S + +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  VSDIVFTMVGHPSDVRQNVLE-KNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCW 128
            SD+VFT+VG+PSDVR  +L+ K+G L GL  GGV+VD T+S P+LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++GKL+IF GGD   VK L PLF  +GKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEY 248
            I   ++GL EGL++A +AGLDVK+F+EA+  GAAGS +++L+G+R+++RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGEDVERL-VVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLN 306
            VKDLG+ ++      + +R+ + LPG ALA+QL+ ++ A+G+G  GTQ L+  ++RLN
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLN 325

[Close]

BLAST of Cp4.1LG01g02050 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-25
Identity = 81/288 (28.12%), Postives = 140/288 (48.61%), Query Frame = 0


Query: 16  IGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFT 75
           +G++G+G+MG AM+  LL  G+ V ++ R+ SK  +L   GAS+  S +E+ +       
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 76  MVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCWAVDAPVSG 135
           M+  P      V +K G+L+ +  G   +D ++     + +I  A   KG   V+ PVSG
Sbjct: 63  MLSDPCAALSVVFDKGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVSG 122

Query: 136 GDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVT-YVGEAGCGQSCKIANQIAIGGNLM 195
               A DG+L I   GD  + +   P F+ LGK + Y+G+ G G   K+   + +G  + 
Sbjct: 123 SKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMMN 182

Query: 196 GLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLGM 255
             SEGLV A+++GL     ++ +  GA  +   +  G  M +  + P    ++  KD+ +
Sbjct: 183 AFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMRL 242

Query: 256 GVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQ 303
            + + +     E  V +P AA A + F    + G G      +I  V+
Sbjct: 243 ALALGD-----ENAVSMPVAAAANEAFKKARSLGLGDLDFSAVIEAVK 285

[Close]

BLAST of Cp4.1LG01g02050 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 114.0 bits (284), Expect = 2.1e-25
Identity = 78/274 (28.47%), Postives = 130/274 (47.45%), Query Frame = 0


Query: 16  IGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFT 75
           IG++G+G+MG+ MA  L+ AG  V ++ R+ SK   L   GA   +S  E+    D+ F 
Sbjct: 69  IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFA 128

Query: 76  MVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAAARAKGCWAVDAPVSG 135
           M+  P         KNG + G++ G   VD ++   A +  I    +  G   ++APVSG
Sbjct: 129 MLADPESAIDVACGKNGAIFGISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAPVSG 188

Query: 136 GDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVT-YVGEAGCGQSCKIANQIAIGGNLM 195
               A DG+L     GD  + +  +P  + +GK   Y+GE G G + K+   + +G  + 
Sbjct: 189 SKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMA 248

Query: 196 GLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLGM 255
             +EG++ +++ GLD    VE V  GA  +    L G  MI+  +      ++  KD+ +
Sbjct: 249 SFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRL 308

Query: 256 GVDVVEEGEDVERLVVLPGAALAKQLFSAMVANG 289
            + + E           P AA A +L+    + G
Sbjct: 309 ALGLAESVSQ-----STPIAAAANELYKVAKSYG 337

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g02050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949M8	1.1e-103	61.76	Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis...	[more]
Q9C991	9.8e-100	59.48	Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis...	[more]
Q9SZE1	7.3e-79	50.17	Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis...	[more]
O34948	1.6e-54	37.98	Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ...	[more]
P77161	1.9e-42	37.59	2-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN...	[more]

BLAST of Cp4.1LG01g02050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023533858.1	1.03e-212	100.00	probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X2 [Cu...	[more]
XP_023533851.1	1.55e-212	100.00	probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X1 [Cu...	[more]
KAG6600356.1	4.87e-212	99.67	putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucu...	[more]
KAG7031015.1	4.00e-211	99.02	putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucu...	[more]
XP_022942972.1	6.98e-211	99.02	probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Cucurbita mos...	[more]

BLAST of Cp4.1LG01g02050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRQ4	3.38e-211	99.02	probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita m...	[more]
A0A6J1J121	1.13e-209	98.70	probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita m...	[more]
A0A6J1D789	6.55e-196	90.88	probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Momordica c...	[more]
A0A6J1FSW9	1.86e-193	89.90	probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita m...	[more]
A0A6J1J1I1	1.03e-190	88.93	probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita m...	[more]

BLAST of Cp4.1LG01g02050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71180.1	7.9e-105	61.76	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71170.1	6.9e-101	59.48	6-phosphogluconate dehydrogenase family protein	[more]
AT4G29120.1	5.2e-80	50.17	6-phosphogluconate dehydrogenase family protein	[more]
AT3G25530.1	1.3e-25	28.13	glyoxylate reductase 1	[more]
AT1G17650.1	2.1e-25	28.47	glyoxylate reductase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029154	3-hydroxyisobutyrate dehydrogenase, NAD-binding domain	PFAM	PF14833	NAD_binding_11	coord: 176..298 e-value: 9.1E-25 score: 87.2
None	No IPR available	GENE3D	3.40.50.720		coord: 9..173 e-value: 1.0E-46 score: 160.8
None	No IPR available	PANTHER	PTHR43060	3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATED	coord: 1..307
None	No IPR available	PANTHER	PTHR43060:SF13	3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATED	coord: 1..307
IPR013328	6-phosphogluconate dehydrogenase, domain 2	GENE3D	1.10.1040.10		coord: 174..307 e-value: 6.2E-36 score: 125.5
IPR015815	3-hydroxyisobutyrate dehydrogenase-related	PIRSF	PIRSF000103	HIBADH	coord: 10..307 e-value: 2.9E-59 score: 198.5
IPR006115	6-phosphogluconate dehydrogenase, NADP-binding	PFAM	PF03446	NAD_binding_2	coord: 15..173 e-value: 1.7E-42 score: 145.3
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 14..172
IPR008927	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily	SUPERFAMILY	48179	6-phosphogluconate dehydrogenase C-terminal domain-like	coord: 174..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g02050.1	Cp4.1LG01g02050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0050661	NADP binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016491	oxidoreductase activity