Cp4.1LG01g01250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g01250 vs. ExPASy Swiss-Prot
Match: O48921 (Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 4.8e-265
Identity = 445/543 (81.95%), Postives = 489/543 (90.06%), Query Frame = 0

Query: 38  LKSNRRGRVLRCQSTQVDEPKTKRNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRP 97
           L   +R   +RCQS   D+ K+ RNLL NASNLLT+LL+GG++GSMPIAEGAVSDL GRP
Sbjct: 34  LSQPKRISSIRCQSINTDKKKSSRNLLGNASNLLTDLLSGGSIGSMPIAEGAVSDLLGRP 93

Query: 98  LFFALYDWFLEHGSVYKLAFGPKAFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGK 157
           LFF+LYDWFLEHG+VYKLAFGPKAFVVVSDPIVAR+ILRENAFSYDKGVLADILEPIMGK
Sbjct: 94  LFFSLYDWFLEHGAVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLADILEPIMGK 153

Query: 158 GLIPADLDTWKQRRRVIAPGFHALYLEAMAKVFADCSERSIRKLEKLLGEGEGQE-DKTI 217
           GLIPADLDTWKQRRRVIAP FH  YLEAM K+F  CSER+I K  KLL EGEG +   +I
Sbjct: 154 GLIPADLDTWKQRRRVIAPAFHNSYLEAMVKIFTTCSERTILKFNKLL-EGEGYDGPDSI 213

Query: 218 ELDMEAEFSNLALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPL 277
           ELD+EAEFS+LALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWK+PL
Sbjct: 214 ELDLEAEFSSLALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKIPL 273

Query: 278 ARWIVPRQRKFHSDLKVINDCLDGLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVD 337
           ARWIVPRQRKF  DLKVIN CLDGLIRNA+E+R+E DVEKLQQRDYLNLKD SLLRFLVD
Sbjct: 274 ARWIVPRQRKFQDDLKVINTCLDGLIRNAKESRQETDVEKLQQRDYLNLKDASLLRFLVD 333

Query: 338 MRGADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRP 397
           MRGADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMK+AQ E+DLVLG GRP
Sbjct: 334 MRGADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKKAQAEVDLVLGTGRP 393

Query: 398 NFELLKELKYIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFIS 457
            FE LKEL+YIRLI+ E LRL+PQPPLLIRR+LK D LPGG+ G+K+GY IPAGTD+FIS
Sbjct: 394 TFESLKELQYIRLIVVEALRLYPQPPLLIRRSLKSDVLPGGHKGEKDGYAIPAGTDVFIS 453

Query: 458 VYNLHRSPYFWDNPQDFEPERFQVNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPF 517
           VYNLHRSPYFWD P DFEPERF V   ++ IEGW G DPSRSPGALYPNE+ISDF+FLPF
Sbjct: 454 VYNLHRSPYFWDRPDDFEPERFLVQNKNEEIEGWAGLDPSRSPGALYPNEVISDFAFLPF 513

Query: 518 GGGPRKCVGDQFALMESTIALAMLLQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRR 577
           GGGPRKCVGDQFALMEST+AL MLLQ FDV+LKG+PESVELVTGATIHTK+G+WC+L++R
Sbjct: 514 GGGPRKCVGDQFALMESTVALTMLLQNFDVELKGTPESVELVTGATIHTKNGMWCRLKKR 573

Query: 578 SQV 580
           S +
Sbjct: 574 SNL 575

BLAST of Cp4.1LG01g01250 vs. ExPASy Swiss-Prot
Match: O23365 (Cytochrome P450 97B3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97B3 PE=1 SV=2)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 431/579 (74.44%), Postives = 493/579 (85.15%), Query Frame = 0

Query: 3   MAASSLSNLPSAVFNG--NLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 62
           MA  + +  P+    G  +L + +    G   +    + S R    ++CQST   EPKT 
Sbjct: 5   MAFPAAATYPTHFQGGALHLGRTDHCLFGFYPQTISSVNSRRASVSIKCQST---EPKTN 64

Query: 63  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 122
            N+LDNASNLLTN L+GG+LGSMP AEG+VSDLFG+PLF +LYDWFLEHG +YKLAFGPK
Sbjct: 65  GNILDNASNLLTNFLSGGSLGSMPTAEGSVSDLFGKPLFLSLYDWFLEHGGIYKLAFGPK 124

Query: 123 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 182
           AFVV+SDPI+AR++LRENAFSYDKGVLA+ILEPIMGKGLIPADLDTWK RRR I P FH 
Sbjct: 125 AFVVISDPIIARHVLRENAFSYDKGVLAEILEPIMGKGLIPADLDTWKLRRRAITPAFHK 184

Query: 183 LYLEAMAKVFADCSERSIRKLEKLLGEGE-GQEDKTIELDMEAEFSNLALDIIGLGVFNY 242
           LYLEAM KVF+DCSE+ I K EKL+ E E    + TIELD+EAEFS+LALDIIGL VFNY
Sbjct: 185 LYLEAMVKVFSDCSEKMILKSEKLIREKETSSGEDTIELDLEAEFSSLALDIIGLSVFNY 244

Query: 243 DFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLD 302
           DFGSVTKESPVIKAVYGTLFEAEHRSTFY PYW  P ARWIVPRQRKF SDLK+INDCLD
Sbjct: 245 DFGSVTKESPVIKAVYGTLFEAEHRSTFYFPYWNFPPARWIVPRQRKFQSDLKIINDCLD 304

Query: 303 GLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGH 362
           GLI+NA+ETR+E DVEKLQ+RDY NLKD SLLRFLVDMRG D+DDRQLRDDLMTMLIAGH
Sbjct: 305 GLIQNAKETRQETDVEKLQERDYTNLKDASLLRFLVDMRGVDIDDRQLRDDLMTMLIAGH 364

Query: 363 ETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFP 422
           ETTAAVLTWAVFLL+QNP K+++AQ EID VLG+G P +E +K+L+YIRLI+ E LRLFP
Sbjct: 365 ETTAAVLTWAVFLLSQNPEKIRKAQAEIDAVLGQGPPTYESMKKLEYIRLIVVEVLRLFP 424

Query: 423 QPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQ 482
           QPPLLIRR LKP+TLPGG+ G+K G+ +P GTDIFISVYNLHRSPYFWDNP DFEPERF 
Sbjct: 425 QPPLLIRRTLKPETLPGGHKGEKEGHKVPKGTDIFISVYNLHRSPYFWDNPHDFEPERFL 484

Query: 483 VNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAM 542
             K S+GIEGW GFDPSRSPGALYPNEII+DF+FLPFGGGPRKC+GDQFALMEST+ALAM
Sbjct: 485 RTKESNGIEGWAGFDPSRSPGALYPNEIIADFAFLPFGGGPRKCIGDQFALMESTVALAM 544

Query: 543 LLQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQ 579
           L QKFDV+L+G+PESVELV+GATIH K+G+WCKL+RRS+
Sbjct: 545 LFQKFDVELRGTPESVELVSGATIHAKNGMWCKLKRRSK 580

BLAST of Cp4.1LG01g01250 vs. ExPASy Swiss-Prot
Match: Q43078 (Cytochrome P450 97B1, chloroplastic OS=Pisum sativum OX=3888 GN=CYP97B1 PE=2 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 2.6e-242
Identity = 408/499 (81.76%), Postives = 452/499 (90.58%), Query Frame = 0

Query: 41  NRRGRVLRCQSTQVDEPK-TKRNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLF 100
           ++R   +RCQS   ++ K + RN+ DNASNLLT+LL+G NLGSMPIAEGAV+DLF RPLF
Sbjct: 45  SKRFSSIRCQSVNGEKRKQSSRNVFDNASNLLTSLLSGANLGSMPIAEGAVTDLFDRPLF 104

Query: 101 FALYDWFLEHGSVYKLAFGPKAFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGL 160
           F+LYDWFLEHGSVYKLAFGPKAFVVVSDPIVAR+ILRENAFSYDKGVLADILEPIMGKGL
Sbjct: 105 FSLYDWFLEHGSVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLADILEPIMGKGL 164

Query: 161 IPADLDTWKQRRRVIAPGFHALYLEAMAKVFADCSERSIRKLEKLLGEGEGQE-DKTIEL 220
           IPADL+TWKQRRRVIAPGFH  YLEAM ++F  CSER++ K+ +LL EGEG++  K++EL
Sbjct: 165 IPADLETWKQRRRVIAPGFHTSYLEAMVQLFTSCSERTVLKVNELL-EGEGRDGQKSVEL 224

Query: 221 DMEAEFSNLALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLAR 280
           D+EAEFSNLAL+IIGLGVFNYDFGSVT ESPVIKAVYGTLFEAEHRSTFYIPYWK PLAR
Sbjct: 225 DLEAEFSNLALEIIGLGVFNYDFGSVTNESPVIKAVYGTLFEAEHRSTFYIPYWKFPLAR 284

Query: 281 WIVPRQRKFHSDLKVINDCLDGLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMR 340
           WIVPRQRKF  DLKVIN CLDGLIRNA+E+R+E DVEKLQQRDY NLKD SLLRFLVDMR
Sbjct: 285 WIVPRQRKFQDDLKVINTCLDGLIRNAKESRQETDVEKLQQRDYSNLKDASLLRFLVDMR 344

Query: 341 GADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNF 400
           G DVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNP KMK+AQ E+DLVLG G+P F
Sbjct: 345 GVDVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPDKMKKAQAEVDLVLGMGKPTF 404

Query: 401 ELLKELKYIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVY 460
           ELLK+L+YIRLI+ ETLRL+PQPPLLIRR+LKPD LPGG+ GDK+GY IPAGTD+FISVY
Sbjct: 405 ELLKKLEYIRLIVVETLRLYPQPPLLIRRSLKPDVLPGGHKGDKDGYTIPAGTDVFISVY 464

Query: 461 NLHRSPYFWDNPQDFEPERFQVNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGG 520
           NLHRSPYFWD P DFEPERF V   ++ +EGW GFDPSRSPGALYPNEIISDF+FLPFGG
Sbjct: 465 NLHRSPYFWDRPNDFEPERFLVQNNNEEVEGWAGFDPSRSPGALYPNEIISDFAFLPFGG 524

Query: 521 GPRKCVGDQFALMESTIAL 538
           GPRKCVGDQFALMEST+AL
Sbjct: 525 GPRKCVGDQFALMESTVAL 542

BLAST of Cp4.1LG01g01250 vs. ExPASy Swiss-Prot
Match: Q93VK5 (Protein LUTEIN DEFICIENT 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97A3 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.1e-123
Identity = 233/497 (46.88%), Postives = 323/497 (64.99%), Query Frame = 0

Query: 83  MPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPKAFVVVSDPIVARYILRENAFSY 142
           +P A+G++  +     F  LY+ FL +G +++L FGPK+F++VSDP +A++IL++NA +Y
Sbjct: 113 VPEAKGSIQAVRNEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAY 172

Query: 143 DKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHALYLEAMAKVFADCSERSIRKLE 202
            KG+LA+IL+ +MGKGLIPAD + W++RRR I P  H  Y+ AM  +F + S+R  +KL+
Sbjct: 173 SKGILAEILDFVMGKGLIPADGEIWRRRRRAIVPALHQKYVAAMISLFGEASDRLCQKLD 232

Query: 203 KLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAE 262
               +GE       E++ME+ FS L LDIIG  VFNYDF S+T ++ VI+AVY  L EAE
Sbjct: 233 AAALKGE-------EVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGVIEAVYTVLREAE 292

Query: 263 HRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDGLIRNARETREEADVEKLQ-QRD 322
            RS   IP W +P+ + I PRQRK  + LK+IND LD LI   +   EE   E+LQ   +
Sbjct: 293 DRSVSPIPVWDIPIWKDISPRQRKVATSLKLINDTLDDLIATCKRMVEE---EELQFHEE 352

Query: 323 YLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMK 382
           Y+N +D S+L FL+   G DV  +QLRDDLMTMLIAGHET+AAVLTW  +LL   PS + 
Sbjct: 353 YMNERDPSILHFLL-ASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLTTEPSVVA 412

Query: 383 RAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGD 442
           + QEE+D V+G   P  + +K+LKY   ++ E+LRL+PQPP+LIRR++  D L       
Sbjct: 413 KLQEEVDSVIGDRFPTIQDMKKLKYTTRVMNESLRLYPQPPVLIRRSIDNDIL------- 472

Query: 443 KNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQVNKVSDGIEGWDGFDPSRSPGA 502
              YPI  G DIFISV+NLHRSP  WD+ + F PER+                P   P  
Sbjct: 473 -GEYPIKRGEDIFISVWNLHRSPLHWDDAEKFNPERW----------------PLDGPN- 532

Query: 503 LYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAMLLQKFDVQLKGSPESVELVTGA 562
             PNE   +FS+LPFGGGPRKC+GD FA  E+ +A+AML+++F+ Q+      V++ TGA
Sbjct: 533 --PNETNQNFSYLPFGGGPRKCIGDMFASFENVVAIAMLIRRFNFQIAPGAPPVKMTTGA 571

Query: 563 TIHTKSGLWCKLRRRSQ 579
           TIHT  GL   + +R++
Sbjct: 593 TIHTTEGLKLTVTKRTK 571

BLAST of Cp4.1LG01g01250 vs. ExPASy Swiss-Prot
Match: Q6TBX7 (Carotene epsilon-monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97C1 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 8.2e-116
Identity = 229/512 (44.73%), Postives = 322/512 (62.89%), Query Frame = 0

Query: 71  LTNLLNGG--NLGSMPIAEGA---VSDLFGRPLFFALYDWFLEHGSVYKLAFGPKAFVVV 130
           LT  L+ G  +   +PIA      V+DL G  LF  LY W  E+G +Y+LA GP+ FV+V
Sbjct: 65  LTRTLSSGKNDESGIPIANAKLDDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIV 124

Query: 131 SDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHALYLEA 190
           SDP +A+++LR N   Y KG++A++ E + G G   A+   W  RRR + P  H  YL  
Sbjct: 125 SDPAIAKHVLR-NYPKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHRRYLSV 184

Query: 191 MA-KVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYDFGSV 250
           +  +VF  C+ER + KL+    +G         ++MEA+FS + LD+IGL +FNY+F S+
Sbjct: 185 IVERVFCKCAERLVEKLQPYAEDGSA-------VNMEAKFSQMTLDVIGLSLFNYNFDSL 244

Query: 251 TKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDGLIRN 310
           T +SPVI+AVY  L EAE RST  +PYWK+     IVPRQ K    + +I + ++ LI  
Sbjct: 245 TTDSPVIEAVYTALKEAELRSTDLLPYWKIDALCKIVPRQVKAEKAVTLIRETVEDLIAK 304

Query: 311 ARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHETTAA 370
            +E   E + E++   +Y+N  D S+LRFL+  R  +V   QLRDDL++ML+AGHETT +
Sbjct: 305 CKEI-VEREGERINDEEYVNDADPSILRFLLASR-EEVSSVQLRDDLLSMLVAGHETTGS 364

Query: 371 VLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQPPLL 430
           VLTW ++LL++N S +++AQEE+D VL    P FE +KELKYI   I E++RL+P PP+L
Sbjct: 365 VLTWTLYLLSKNSSALRKAQEEVDRVLEGRNPAFEDIKELKYITRCINESMRLYPHPPVL 424

Query: 431 IRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQVNKVS 490
           IRRA  PD LPG Y        +  G DI ISVYN+HRS   W+  ++F PERF ++   
Sbjct: 425 IRRAQVPDILPGNYK-------VNTGQDIMISVYNIHRSSEVWEKAEEFLPERFDID--- 484

Query: 491 DGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAMLLQKF 550
                          GA+ PNE  +DF F+PF GGPRKCVGDQFALME+ +ALA+ LQ+ 
Sbjct: 485 ---------------GAI-PNETNTDFKFIPFSGGPRKCVGDQFALMEAIVALAVFLQRL 539

Query: 551 DVQLKGSPESVELVTGATIHTKSGLWCKLRRR 577
           +V+L    +++ + TGATIHT +GL+ K+ +R
Sbjct: 545 NVELV-PDQTISMTTGATIHTTNGLYMKVSQR 539

BLAST of Cp4.1LG01g01250 vs. NCBI nr
Match: XP_023529541.1 (cytochrome P450 97B2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. NCBI nr
Match: XP_022980002.1 (cytochrome P450 97B2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 573/580 (98.79%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGE  EDKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEVHEDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKF+SDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFNSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQEE+DLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQEEVDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NK SDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKASDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. NCBI nr
Match: XP_022942292.1 (cytochrome P450 97B2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 571/580 (98.45%), Postives = 575/580 (99.14%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRR RVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRARVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGE  EDKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEVHEDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQEE+DLVLGKGRPNFELLKELKYIRLI+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQEEVDLVLGKGRPNFELLKELKYIRLIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNP+DFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPRDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NK SDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKASDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. NCBI nr
Match: KAG6600440.1 (Cytochrome P450 97B2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1154 bits (2984), Expect = 0.0
Identity = 570/580 (98.28%), Postives = 574/580 (98.97%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRP KYLKSNRRGRVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPTKYLKSNRRGRVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGE  +DKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEVHKDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQEE+DLVLGKGRPNFELLKELKYIRLI+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQEEVDLVLGKGRPNFELLKELKYIRLIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNP DFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPPDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NK SDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKASDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. NCBI nr
Match: XP_038897993.1 (cytochrome P450 97B2, chloroplastic [Benincasa hispida] >XP_038898000.1 cytochrome P450 97B2, chloroplastic [Benincasa hispida] >XP_038898005.1 cytochrome P450 97B2, chloroplastic [Benincasa hispida] >XP_038898013.1 cytochrome P450 97B2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 535/580 (92.24%), Postives = 556/580 (95.86%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           M MAA+SLSNLPSA F+GNLQ+NEFG++G+PR+P K+LKSN R  + RCQST VDEPKTK
Sbjct: 1   MAMAATSLSNLPSAFFHGNLQRNEFGYLGLPRQPTKFLKSNLRAPLFRCQSTGVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLL+GGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLSGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAF YDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFGYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAM KVF DCSERSI KLEKLLGEGE QE+KTIELDMEAEFS+LALDIIGLGVFNYD
Sbjct: 181 LYLEAMTKVFTDCSERSILKLEKLLGEGELQEEKTIELDMEAEFSSLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRG DVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGVDVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQ EIDLVLG GRP FELLK LKYIRLI+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQAEIDLVLGSGRPTFELLKGLKYIRLIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGY IPAGTDIFISVYNLHRSPYFW NPQ+FEPERF+V
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYAIPAGTDIFISVYNLHRSPYFWANPQEFEPERFKV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
            + S+GIEGWDGFDPSRSPGALYPNEI+SDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 KRTSEGIEGWDGFDPSRSPGALYPNEIVSDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDV+L+GSPESVELVTGATIHTKSGLWCKLRRR QVN
Sbjct: 541 LQKFDVELRGSPESVELVTGATIHTKSGLWCKLRRRLQVN 580

BLAST of Cp4.1LG01g01250 vs. ExPASy TrEMBL
Match: A0A6J1IQ92 (cytochrome P450 97B2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111479533 PE=3 SV=1)

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 573/580 (98.79%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGE  EDKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEVHEDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKF+SDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFNSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQEE+DLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQEEVDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NK SDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKASDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. ExPASy TrEMBL
Match: A0A6J1FPU1 (cytochrome P450 97B2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447380 PE=3 SV=1)

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 571/580 (98.45%), Postives = 575/580 (99.14%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRR RVLRCQSTQVDEPKTK
Sbjct: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRARVLRCQSTQVDEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA
Sbjct: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
           LYLEAMAKVFADCSERSIRKLEKLLGEGE  EDKTIELDMEAEFSNLALDIIGLGVFNYD
Sbjct: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEVHEDKTIELDMEAEFSNLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQEE+DLVLGKGRPNFELLKELKYIRLI+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQEEVDLVLGKGRPNFELLKELKYIRLIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNP+DFEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPRDFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
           NK SDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 NKASDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. ExPASy TrEMBL
Match: A0A0A0KVB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056760 PE=3 SV=1)

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 532/580 (91.72%), Postives = 554/580 (95.52%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           M MAA++L NLPSA+F+GN  +NE GF+GM R+P KYLK N R  V+RCQST V EPKTK
Sbjct: 1   MAMAATALWNLPSALFHGNPHRNEIGFLGMSRQPIKYLKYNLRAPVVRCQSTDVGEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLL+GGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLSGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVA+YILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFH 
Sbjct: 121 AFVVVSDPIVAKYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHT 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
            YLEAM KVFADCSERSI KLEKLLGEGE Q+DKTIELDMEAEFS+LALDIIGLGVFNYD
Sbjct: 181 YYLEAMTKVFADCSERSILKLEKLLGEGELQKDKTIELDMEAEFSSLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRG DVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGVDVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQ EIDLVLGKG+P FEL KELKYIRLI+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQAEIDLVLGKGKPTFELFKELKYIRLIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGY IPAGTDIFISVYNLHRSPYFW+NPQ+FEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYAIPAGTDIFISVYNLHRSPYFWENPQEFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
            + S+GIEGWDGFDPSRSPGALYPNEI+SDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 KRASEGIEGWDGFDPSRSPGALYPNEIVSDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580
           LQKFDV+L+GSPESVELVTGATIHTKSGLWCKLRRRSQVN
Sbjct: 541 LQKFDVELRGSPESVELVTGATIHTKSGLWCKLRRRSQVN 580

BLAST of Cp4.1LG01g01250 vs. ExPASy TrEMBL
Match: A0A5D3CYA2 (Cytochrome P450 97B2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002780 PE=3 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 530/579 (91.54%), Postives = 551/579 (95.16%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           M M A++LSNLPSA+F+GN   NEFGF+GM ++P KYLKSN R  V+RCQST V EPKTK
Sbjct: 1   MAMVATALSNLPSALFHGNPHGNEFGFLGMSKQPIKYLKSNLRAPVVRCQSTDVGEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLL+GGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLSGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAF+YDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFH 
Sbjct: 121 AFVVVSDPIVARYILRENAFAYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHT 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
            YLEAM KVF DCSERSI KLEKLLGEGE QE KTIELDMEAEFS+LALDIIGLGVFNYD
Sbjct: 181 YYLEAMTKVFTDCSERSILKLEKLLGEGELQEAKTIELDMEAEFSSLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRG DVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGVDVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQ EID VLGKG+P FELLKELKYIR I+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQAEIDSVLGKGKPTFELLKELKYIRFIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGY IPAGTDIFISVYNLHRSPYFW+NPQ+FEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYAIPAGTDIFISVYNLHRSPYFWENPQEFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
            + S+GIEGWDGFDPSRSPGALYPNEI+SDFSFLPFGGGPRKCVGDQFALMESTIALAML
Sbjct: 481 KRASEGIEGWDGFDPSRSPGALYPNEIVSDFSFLPFGGGPRKCVGDQFALMESTIALAML 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQV 579
           LQKFDV+L+GSPESVELVTGATIHTKSGLWCKLRRRSQV
Sbjct: 541 LQKFDVELRGSPESVELVTGATIHTKSGLWCKLRRRSQV 579

BLAST of Cp4.1LG01g01250 vs. ExPASy TrEMBL
Match: A0A1S4DYV6 (LOW QUALITY PROTEIN: cytochrome P450 97B2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493048 PE=3 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 529/579 (91.36%), Postives = 550/579 (94.99%), Query Frame = 0

Query: 1   MVMAASSLSNLPSAVFNGNLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 60
           M M A++LSNLPSA+F+GN   NEFGF+GM ++P KYLKSN R  V+RCQST V EPKTK
Sbjct: 1   MAMVATALSNLPSALFHGNPHGNEFGFLGMSKQPIKYLKSNLRAPVVRCQSTDVGEPKTK 60

Query: 61  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120
           RNLLDNASNLLTNLL+GGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK
Sbjct: 61  RNLLDNASNLLTNLLSGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 120

Query: 121 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 180
           AFVVVSDPIVARYILRENAF+YDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFH 
Sbjct: 121 AFVVVSDPIVARYILRENAFAYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHT 180

Query: 181 LYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYD 240
            YLEAM KVF DCSERSI KLEKLLGEGE QE KTIELDMEAEFS+LALDIIGLGVFNYD
Sbjct: 181 YYLEAMTKVFTDCSERSILKLEKLLGEGELQEAKTIELDMEAEFSSLALDIIGLGVFNYD 240

Query: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300
           FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG
Sbjct: 241 FGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDG 300

Query: 301 LIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHE 360
           LIRNARETREEADVEKLQQRDYLNLKD SLLRFLVDMRG DVDDRQLRDDLMTMLIAGHE
Sbjct: 301 LIRNARETREEADVEKLQQRDYLNLKDASLLRFLVDMRGVDVDDRQLRDDLMTMLIAGHE 360

Query: 361 TTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQ 420
           TTAAVLTWAVFLLAQNPSKMK+AQ EID VLGKG+P FELLKELKYIR I+AETLRLFPQ
Sbjct: 361 TTAAVLTWAVFLLAQNPSKMKKAQAEIDSVLGKGKPTFELLKELKYIRFIVAETLRLFPQ 420

Query: 421 PPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQV 480
           PPLLIRRALKPDTLPGGYNGDKNGY IPAGTDIFISVYNLHRSPYFW+NPQ+FEPERFQV
Sbjct: 421 PPLLIRRALKPDTLPGGYNGDKNGYAIPAGTDIFISVYNLHRSPYFWENPQEFEPERFQV 480

Query: 481 NKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAML 540
            + S+GIEGWDGFDPSRSPGALYPNEI+SDFSFLPFGGGPRKCVGDQFALMESTIALA L
Sbjct: 481 KRASEGIEGWDGFDPSRSPGALYPNEIVSDFSFLPFGGGPRKCVGDQFALMESTIALAXL 540

Query: 541 LQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQV 579
           LQKFDV+L+GSPESVELVTGATIHTKSGLWCKLRRRSQV
Sbjct: 541 LQKFDVELRGSPESVELVTGATIHTKSGLWCKLRRRSQV 579

BLAST of Cp4.1LG01g01250 vs. TAIR 10
Match: AT4G15110.1 (cytochrome P450, family 97, subfamily B, polypeptide 3 )

HSP 1 Score: 889.4 bits (2297), Expect = 1.5e-258
Identity = 431/579 (74.44%), Postives = 493/579 (85.15%), Query Frame = 0

Query: 3   MAASSLSNLPSAVFNG--NLQKNEFGFVGMPRRPAKYLKSNRRGRVLRCQSTQVDEPKTK 62
           MA  + +  P+    G  +L + +    G   +    + S R    ++CQST   EPKT 
Sbjct: 5   MAFPAAATYPTHFQGGALHLGRTDHCLFGFYPQTISSVNSRRASVSIKCQST---EPKTN 64

Query: 63  RNLLDNASNLLTNLLNGGNLGSMPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPK 122
            N+LDNASNLLTN L+GG+LGSMP AEG+VSDLFG+PLF +LYDWFLEHG +YKLAFGPK
Sbjct: 65  GNILDNASNLLTNFLSGGSLGSMPTAEGSVSDLFGKPLFLSLYDWFLEHGGIYKLAFGPK 124

Query: 123 AFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHA 182
           AFVV+SDPI+AR++LRENAFSYDKGVLA+ILEPIMGKGLIPADLDTWK RRR I P FH 
Sbjct: 125 AFVVISDPIIARHVLRENAFSYDKGVLAEILEPIMGKGLIPADLDTWKLRRRAITPAFHK 184

Query: 183 LYLEAMAKVFADCSERSIRKLEKLLGEGE-GQEDKTIELDMEAEFSNLALDIIGLGVFNY 242
           LYLEAM KVF+DCSE+ I K EKL+ E E    + TIELD+EAEFS+LALDIIGL VFNY
Sbjct: 185 LYLEAMVKVFSDCSEKMILKSEKLIREKETSSGEDTIELDLEAEFSSLALDIIGLSVFNY 244

Query: 243 DFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLD 302
           DFGSVTKESPVIKAVYGTLFEAEHRSTFY PYW  P ARWIVPRQRKF SDLK+INDCLD
Sbjct: 245 DFGSVTKESPVIKAVYGTLFEAEHRSTFYFPYWNFPPARWIVPRQRKFQSDLKIINDCLD 304

Query: 303 GLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGH 362
           GLI+NA+ETR+E DVEKLQ+RDY NLKD SLLRFLVDMRG D+DDRQLRDDLMTMLIAGH
Sbjct: 305 GLIQNAKETRQETDVEKLQERDYTNLKDASLLRFLVDMRGVDIDDRQLRDDLMTMLIAGH 364

Query: 363 ETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFP 422
           ETTAAVLTWAVFLL+QNP K+++AQ EID VLG+G P +E +K+L+YIRLI+ E LRLFP
Sbjct: 365 ETTAAVLTWAVFLLSQNPEKIRKAQAEIDAVLGQGPPTYESMKKLEYIRLIVVEVLRLFP 424

Query: 423 QPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQ 482
           QPPLLIRR LKP+TLPGG+ G+K G+ +P GTDIFISVYNLHRSPYFWDNP DFEPERF 
Sbjct: 425 QPPLLIRRTLKPETLPGGHKGEKEGHKVPKGTDIFISVYNLHRSPYFWDNPHDFEPERFL 484

Query: 483 VNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAM 542
             K S+GIEGW GFDPSRSPGALYPNEII+DF+FLPFGGGPRKC+GDQFALMEST+ALAM
Sbjct: 485 RTKESNGIEGWAGFDPSRSPGALYPNEIIADFAFLPFGGGPRKCIGDQFALMESTVALAM 544

Query: 543 LLQKFDVQLKGSPESVELVTGATIHTKSGLWCKLRRRSQ 579
           L QKFDV+L+G+PESVELV+GATIH K+G+WCKL+RRS+
Sbjct: 545 LFQKFDVELRGTPESVELVSGATIHAKNGMWCKLKRRSK 580

BLAST of Cp4.1LG01g01250 vs. TAIR 10
Match: AT1G31800.1 (cytochrome P450, family 97, subfamily A, polypeptide 3 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.2e-124
Identity = 233/497 (46.88%), Postives = 323/497 (64.99%), Query Frame = 0

Query: 83  MPIAEGAVSDLFGRPLFFALYDWFLEHGSVYKLAFGPKAFVVVSDPIVARYILRENAFSY 142
           +P A+G++  +     F  LY+ FL +G +++L FGPK+F++VSDP +A++IL++NA +Y
Sbjct: 113 VPEAKGSIQAVRNEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAY 172

Query: 143 DKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHALYLEAMAKVFADCSERSIRKLE 202
            KG+LA+IL+ +MGKGLIPAD + W++RRR I P  H  Y+ AM  +F + S+R  +KL+
Sbjct: 173 SKGILAEILDFVMGKGLIPADGEIWRRRRRAIVPALHQKYVAAMISLFGEASDRLCQKLD 232

Query: 203 KLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAE 262
               +GE       E++ME+ FS L LDIIG  VFNYDF S+T ++ VI+AVY  L EAE
Sbjct: 233 AAALKGE-------EVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGVIEAVYTVLREAE 292

Query: 263 HRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDGLIRNARETREEADVEKLQ-QRD 322
            RS   IP W +P+ + I PRQRK  + LK+IND LD LI   +   EE   E+LQ   +
Sbjct: 293 DRSVSPIPVWDIPIWKDISPRQRKVATSLKLINDTLDDLIATCKRMVEE---EELQFHEE 352

Query: 323 YLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMK 382
           Y+N +D S+L FL+   G DV  +QLRDDLMTMLIAGHET+AAVLTW  +LL   PS + 
Sbjct: 353 YMNERDPSILHFLL-ASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLTTEPSVVA 412

Query: 383 RAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGD 442
           + QEE+D V+G   P  + +K+LKY   ++ E+LRL+PQPP+LIRR++  D L       
Sbjct: 413 KLQEEVDSVIGDRFPTIQDMKKLKYTTRVMNESLRLYPQPPVLIRRSIDNDIL------- 472

Query: 443 KNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQVNKVSDGIEGWDGFDPSRSPGA 502
              YPI  G DIFISV+NLHRSP  WD+ + F PER+                P   P  
Sbjct: 473 -GEYPIKRGEDIFISVWNLHRSPLHWDDAEKFNPERW----------------PLDGPN- 532

Query: 503 LYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAMLLQKFDVQLKGSPESVELVTGA 562
             PNE   +FS+LPFGGGPRKC+GD FA  E+ +A+AML+++F+ Q+      V++ TGA
Sbjct: 533 --PNETNQNFSYLPFGGGPRKCIGDMFASFENVVAIAMLIRRFNFQIAPGAPPVKMTTGA 571

Query: 563 TIHTKSGLWCKLRRRSQ 579
           TIHT  GL   + +R++
Sbjct: 593 TIHTTEGLKLTVTKRTK 571

BLAST of Cp4.1LG01g01250 vs. TAIR 10
Match: AT3G53130.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 5.9e-117
Identity = 229/512 (44.73%), Postives = 322/512 (62.89%), Query Frame = 0

Query: 71  LTNLLNGG--NLGSMPIAEGA---VSDLFGRPLFFALYDWFLEHGSVYKLAFGPKAFVVV 130
           LT  L+ G  +   +PIA      V+DL G  LF  LY W  E+G +Y+LA GP+ FV+V
Sbjct: 65  LTRTLSSGKNDESGIPIANAKLDDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIV 124

Query: 131 SDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWKQRRRVIAPGFHALYLEA 190
           SDP +A+++LR N   Y KG++A++ E + G G   A+   W  RRR + P  H  YL  
Sbjct: 125 SDPAIAKHVLR-NYPKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHRRYLSV 184

Query: 191 MA-KVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLALDIIGLGVFNYDFGSV 250
           +  +VF  C+ER + KL+    +G         ++MEA+FS + LD+IGL +FNY+F S+
Sbjct: 185 IVERVFCKCAERLVEKLQPYAEDGSA-------VNMEAKFSQMTLDVIGLSLFNYNFDSL 244

Query: 251 TKESPVIKAVYGTLFEAEHRSTFYIPYWKVPLARWIVPRQRKFHSDLKVINDCLDGLIRN 310
           T +SPVI+AVY  L EAE RST  +PYWK+     IVPRQ K    + +I + ++ LI  
Sbjct: 245 TTDSPVIEAVYTALKEAELRSTDLLPYWKIDALCKIVPRQVKAEKAVTLIRETVEDLIAK 304

Query: 311 ARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDRQLRDDLMTMLIAGHETTAA 370
            +E   E + E++   +Y+N  D S+LRFL+  R  +V   QLRDDL++ML+AGHETT +
Sbjct: 305 CKEI-VEREGERINDEEYVNDADPSILRFLLASR-EEVSSVQLRDDLLSMLVAGHETTGS 364

Query: 371 VLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELKYIRLIIAETLRLFPQPPLL 430
           VLTW ++LL++N S +++AQEE+D VL    P FE +KELKYI   I E++RL+P PP+L
Sbjct: 365 VLTWTLYLLSKNSSALRKAQEEVDRVLEGRNPAFEDIKELKYITRCINESMRLYPHPPVL 424

Query: 431 IRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPYFWDNPQDFEPERFQVNKVS 490
           IRRA  PD LPG Y        +  G DI ISVYN+HRS   W+  ++F PERF ++   
Sbjct: 425 IRRAQVPDILPGNYK-------VNTGQDIMISVYNIHRSSEVWEKAEEFLPERFDID--- 484

Query: 491 DGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCVGDQFALMESTIALAMLLQKF 550
                          GA+ PNE  +DF F+PF GGPRKCVGDQFALME+ +ALA+ LQ+ 
Sbjct: 485 ---------------GAI-PNETNTDFKFIPFSGGPRKCVGDQFALMEAIVALAVFLQRL 539

Query: 551 DVQLKGSPESVELVTGATIHTKSGLWCKLRRR 577
           +V+L    +++ + TGATIHT +GL+ K+ +R
Sbjct: 545 NVELV-PDQTISMTTGATIHTTNGLYMKVSQR 539

BLAST of Cp4.1LG01g01250 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 156.8 bits (395), Expect = 5.4e-38
Identity = 119/447 (26.62%), Postives = 200/447 (44.74%), Query Frame = 0

Query: 109 HGSVYKLAFGPKAFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWK 168
           HG      FGP   + + DP   + +  +  + + K      L  I+G GL+  D D W 
Sbjct: 92  HGRTNLTWFGPIPTITIMDPEQIKEVFNK-VYDFQKAHTFP-LSKILGTGLVSYDGDKWA 151

Query: 169 QRRRVIAPGFHALYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLA 228
           Q RR+I P FH   ++ M  VF +     + + +KL+ +    +  + E+D+    +++ 
Sbjct: 152 QHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSD----KGSSCEVDVWPGLTSMT 211

Query: 229 LDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRS--TFYIP-YWKVPLARWIVPRQR 288
            D+I        FGS  +E   I  +   L +   ++   F+IP Y  +P         R
Sbjct: 212 ADVIS----RTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLP-----TKGNR 271

Query: 289 KFHSDLKVINDCLDGLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDR 348
           +  +  + I D L G+I      RE  +          +L  + L   L    G  +   
Sbjct: 272 RMKTAAREIQDILRGIINKRERARESGEAPS------EDLLGILLESNLGQTEGNGMSTE 331

Query: 349 QLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELK 408
            + ++     +AG ETT+ +L W + LL+Q+     RA+EE+  V G  +P+ E L +LK
Sbjct: 332 DMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLK 391

Query: 409 YIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPY 468
            + +I+ E LRL+P P + + RA+  +   G          +P G  I + V  +HR   
Sbjct: 392 VMTMILYEVLRLYP-PVVQLTRAIHKEMKLGDLT-------LPGGVQISLPVLLVHRDTE 451

Query: 469 FWDNPQ-DFEPERFQVNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCV 528
            W N   +F+PERF+           DG   +            +  SF PF  GPR C+
Sbjct: 452 LWGNDAGEFKPERFK-----------DGLSKATK----------NQVSFFPFAWGPRICI 488

Query: 529 GDQFALMESTIALAMLLQKFDVQLKGS 552
           G  F L+E+ +A++++LQ+F  +L  S
Sbjct: 512 GQNFTLLEAKMAMSLILQRFSFELSPS 488

BLAST of Cp4.1LG01g01250 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 151.8 bits (382), Expect = 1.7e-36
Identity = 128/472 (27.12%), Postives = 209/472 (44.28%), Query Frame = 0

Query: 109 HGSVYKLAFGPKAFVVVSDPIVARYILRENAFSYDKGVLADILEPIMGKGLIPADLDTWK 168
           HG  +    GPK  + + DP + + +  +  + Y K     +L  ++  G+I  D D W 
Sbjct: 92  HGRTFFTWLGPKPTITIMDPELIKEVFNK-VYDYPK-AQTFLLGRLIATGIINYDGDKWA 151

Query: 169 QRRRVIAPGFHALYLEAMAKVFADCSERSIRKLEKLLGEGEGQEDKTIELDMEAEFSNLA 228
           + RR+I P FH   ++ M   F       + +  KL+ +   +   + E+D+     ++ 
Sbjct: 152 KHRRIINPAFHIEKIKNMVPAFHQSCSDVVGEWSKLVSD---KGSSSCEVDVWPWLVSMT 211

Query: 229 LDIIGLGVFNYDFGSVTKESPVIKAVYGTLFEAEHRSTFYIPYWKV--PLARWIVPR-QR 288
            D+I        FGS  KE   I  +     +AE        +WKV  P  R++  +  R
Sbjct: 212 GDVIS----RTAFGSSYKEGQRIFEL-----QAELVHLILQAFWKVYIPGYRYLPTKSNR 271

Query: 289 KFHSDLKVINDCLDGLIRNARETREEADVEKLQQRDYLNLKDVSLLRFLVDMRGADVDDR 348
           +  +  + I   L G++      RE     K    D  +L  + L   L   +G  +   
Sbjct: 272 RMKAAAREIQVILKGIVNKRLRAREAG---KAAPND--DLLGILLESNLGQAKGNGMSTE 331

Query: 349 QLRDDLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKRAQEEIDLVLGKGRPNFELLKELK 408
            + ++      AG ETT+ +L WA+ LL+ +     RA+EE+  V G   P+ E L +LK
Sbjct: 332 DVMEECKLFYFAGQETTSVLLVWAMVLLSHHQDWQARAREEVKQVFGDKEPDTECLSQLK 391

Query: 409 YIRLIIAETLRLFPQPPLLIRRALKPDTLPGGYNGDKNGYPIPAGTDIFISVYNLHRSPY 468
            + +I+ E LRL+P P   + RA+  +   G          +PAG  I + +  + R P 
Sbjct: 392 VMTMILYEVLRLYP-PVTHLTRAIDKEMKLGDLT-------LPAGVHISLPIMLVQRDPM 451

Query: 469 FW-DNPQDFEPERFQVNKVSDGIEGWDGFDPSRSPGALYPNEIISDFSFLPFGGGPRKCV 528
            W  +  +F+PERF+           DG   +            S  SF PF  GPR C+
Sbjct: 452 LWGTDAAEFKPERFK-----------DGLSKATK----------SQVSFFPFAWGPRICI 511

Query: 529 GDQFALMESTIALAMLLQKFDVQLKGSPESVEL-VTGATIHTKSGLWCKLRR 576
           G  FA++E+ +A+A++LQ F  +L  SP  V    T  TIH + G    LR+
Sbjct: 512 GQNFAMLEAKMAMALILQTFTFEL--SPSYVHAPQTVVTIHPQFGAHLILRK 513