Cp4.1LG01g01120 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g01120
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	nine-cis-epoxycarotenoid dioxygenase 4
Location	Cp4.1LG01: 3150152 .. 3151912 (-)
RNA-Seq Expression	Cp4.1LG01g01120
Synteny	Cp4.1LG01g01120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGCCATTTTTTCCCCTTTCCTCACCGGCGGAAACCTCCTCCTCTCTCCGCCAATCTCCACCGCCCGACCGCCCTTTTCTGTGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCCGATTCTCCATCTCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGCTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGCCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCATTTGTTCGACGGCGACGGGATGCTTCACTCGCTTCGAATCTCCAACGGCCGAGCCGTTCTGTGCAGTCGTTATGTCAAGACTTACAAATACACTCTGGAGCGTGATGCTGGTCATCCTGTTTTCCCCAATGTTTTCTCCGGCTTCAATGGACTCACAGCCTCCGCCGCTCGGGGCGCCGTCGCAGCCGGCCGGGTGTTAACCGGCCAGTACAATCCGGCAAACGGCATTGGCCTTGCCAACACTAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATCCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTGCCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAACCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGAAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACGCTGGAGAGAATGGATCTGGTTCACGCCTTAATCGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACCTAGACTTCGGAGTGATAAATCCGTCGTACATCGGGAAGAAGAACAGGTTCATATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAGCAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACGAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGATGAAAACTCCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGGCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAACTGATCTCAATAAGCTATGA

mRNA sequence

ATGGACGCCATTTTTTCCCCTTTCCTCACCGGCGGAAACCTCCTCCTCTCTCCGCCAATCTCCACCGCCCGACCGCCCTTTTCTGTGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCCGATTCTCCATCTCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGCTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGCCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCATTTGTTCGACGGCGACGGGATGCTTCACTCGCTTCGAATCTCCAACGGCCGAGCCGTTCTGTGCAGTCGTTATGTCAAGACTTACAAATACACTCTGGAGCGTGATGCTGGTCATCCTGTTTTCCCCAATGTTTTCTCCGGCTTCAATGGACTCACAGCCTCCGCCGCTCGGGGCGCCGTCGCAGCCGGCCGGGTGTTAACCGGCCAGTACAATCCGGCAAACGGCATTGGCCTTGCCAACACTAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATCCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTGCCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAACCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGAAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACGCTGGAGAGAATGGATCTGGTTCACGCCTTAATCGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACCTAGACTTCGGAGTGATAAATCCGTCGTACATCGGGAAGAAGAACAGGTTCATATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAGCAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACGAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGATGAAAACTCCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGGCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAACTGATCTCAATAAGCTATGA

Coding sequence (CDS)

ATGGACGCCATTTTTTCCCCTTTCCTCACCGGCGGAAACCTCCTCCTCTCTCCGCCAATCTCCACCGCCCGACCGCCCTTTTCTGTGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCCGATTCTCCATCTCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGCTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGCCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCATTTGTTCGACGGCGACGGGATGCTTCACTCGCTTCGAATCTCCAACGGCCGAGCCGTTCTGTGCAGTCGTTATGTCAAGACTTACAAATACACTCTGGAGCGTGATGCTGGTCATCCTGTTTTCCCCAATGTTTTCTCCGGCTTCAATGGACTCACAGCCTCCGCCGCTCGGGGCGCCGTCGCAGCCGGCCGGGTGTTAACCGGCCAGTACAATCCGGCAAACGGCATTGGCCTTGCCAACACTAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATCCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTGCCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAACCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGAAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACGCTGGAGAGAATGGATCTGGTTCACGCCTTAATCGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACCTAGACTTCGGAGTGATAAATCCGTCGTACATCGGGAAGAAGAACAGGTTCATATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAGCAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACGAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGATGAAAACTCCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGGCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAACTGATCTCAATAAGCTATGA

Protein sequence

MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL

Homology

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BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 7.3e-229
Identity = 384/574 (66.90%), Postives = 461/574 (80.31%), Query Frame = 0


Query: 26  PFSVAITSVFTEETPKTVKKTNADSPSPRRPPPP--------AMAKGPSTTRLEPSLPAR 85
           P  + I S   EE       TN    + RR  P          +   P   R E +L   
Sbjct: 28  PTLLRINSAVVEERSPI---TNPSDNNDRRNKPKTLHNRTNHTLVSSPPKLRPEMTLATA 87

Query: 86  FFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTECEVIQGSLPSSLNGAYIR 145
            F   +D+IN FI+PP  PSVDP++VL+DNFAPV DELPPT+CE+I G+LP SLNGAYIR
Sbjct: 88  LFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGAYIR 147

Query: 146 NGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFS 205
           NGPNPQ+LPRGPYHLFDGDGMLH+++I NG+A LCSRYVKTYKY +E+  G PV PNVFS
Sbjct: 148 NGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPNVFS 207

Query: 206 GFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAP 265
           GFNG+TAS ARGA+ A RVLTGQYNP NGIGLANTS+AFF +RL+ALGESDLPY++RL  
Sbjct: 208 GFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVRLTE 267

Query: 266 NGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQSDV 325
           +G+IET+GR DFDG L MSMTAHPK+DP TGETFAFRYGP+PPFLT+FRFD  G KQ DV
Sbjct: 268 SGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQRDV 327

Query: 326 PIFSMNRPSFLHDFAITKKYAVFTEIQIG--FNPMLMIMEGRSPVGTDPSTVSRVGLIPR 385
           PIFSM  PSFLHDFAITK++A+F EIQ+G   N + +++EG SPVGTD     R+G+IP+
Sbjct: 328 PIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGVIPK 387

Query: 386 YANDESKMKWFDVPGFNLIHAINAWDEDD--AVVLLAPNILSVEHTLERMDLVHALIEEV 445
           YA DES+MKWF+VPGFN+IHAINAWDEDD  +VVL+APNI+S+EHTLERMDLVHAL+E+V
Sbjct: 388 YAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLERMDLVHALVEKV 447

Query: 446 RIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQER 505
           +IDL TGIV R P+S RNLDF VINP+++G+ +R++YA +GDPMPKISGVVKLDVS+ +R
Sbjct: 448 KIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSKGDR 507

Query: 506 RDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDA 565
            DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE +GESKF+VMDA
Sbjct: 508 DDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDEVTGESKFLVMDA 567

Query: 566 KSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 587
           KSP+LEI+A V+LPRRVPYGFHGLFVKE+DLNKL
Sbjct: 568 KSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-197
Identity = 350/588 (59.52%), Postives = 436/588 (74.15%), Query Frame = 0


Query: 14  LLLSPPISTARPPFSVAITS---VFTEETPKTVKKTN----ADSPS-PRRPPPPAMAKGP 73
           +L SP ++T R P          V   +T +TV  T     A S S PRR P PA A   
Sbjct: 57  VLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAPAAAASA 116

Query: 74  STTRLEPSLPARFFNAFDDLINNFINPP-VSPSVDPRYVLADNFAPVDELPPTECEVIQG 133
           +      SLP  F NA +++IN FI+PP + P+VDPR VL  NF PVDELPPT C V++G
Sbjct: 117 A------SLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCPVVRG 176

Query: 134 SLPSSL-NGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI-----SNGRAVLCSRYVKTY 193
           ++P  L  GAYIRNGPNPQ+LPRGP+HLFDGDGMLHSL +     S    VLCSRYV+TY
Sbjct: 177 AIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRYVQTY 236

Query: 194 KYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGD 253
           KY +ERDAG PV PNVFSGF+G+ A  ARGAV A RVLTGQ NP  G+GLANTS+A+F  
Sbjct: 237 KYLVERDAGAPVLPNVFSGFHGV-AGMARGAVVAARVLTGQMNPLEGVGLANTSLAYFAG 296

Query: 254 RLYALGESDLPYSIRLAPN-GEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPL 313
           RLYALGESDLPY++R+ P+ GE+ T GR DF G L M MTAHPK DP TGE FAFRYGP+
Sbjct: 297 RLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRYGPV 356

Query: 314 PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRS 373
           PPF+T+FRFD  G K +DVPIFS+ +PSFLHDFAIT++YA+F EIQI   PM M++ G S
Sbjct: 357 PPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVGGGS 416

Query: 374 PVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDE--DDAVVLLAPNILSV 433
           PVG+DP  V R+G+IPRYA DES+M+WF+VPGFN++H++NAW+E   + +VL+APN+LS+
Sbjct: 417 PVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNVLSI 476

Query: 434 EHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDP 493
           EH LE M+LVH+ +E+VRI+L+TG+VTR PL+  N DF VINP+++G++NR+ Y GVGDP
Sbjct: 477 EHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFGVGDP 536

Query: 494 MPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVS 553
            PKI GV KLD  +    DC VA R FGPGC+ GEPFFV  + E +      EDDGY+V 
Sbjct: 537 APKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGNG----NEDDGYLVC 596

Query: 554 YVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDL 584
           YVHDE +GE++F+VMDA+SP LEI+A V+LP RVPYGFHGLFV + +L
Sbjct: 597 YVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAEL 633

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.1e-165
Identity = 295/523 (56.41%), Postives = 368/523 (70.36%), Query Frame = 0


Query: 73  SLPARFFNAFDDLINNFIN-PPVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLPSSL- 132
           SLP    N  D +IN FI+ P   P+VDPR VL+ NFAPVDELPPT C VI+GS+PS L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 133 NGAYIRNGPNPQY-LPRGPYHLFDGDGMLHSLRISNGRA------VLCSRYVKTYKYTLE 192
            G YIRNGPNPQ+ LP+  +HLFDGDGMLHSL I +  +      VLCSRYV TYKY LE
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 193 RDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYAL 252
           R+ G PV PN F+GF+G+ A  AR  V   RVL GQ N   G GLANTS+  F D LYAL
Sbjct: 175 RETGGPVLPNFFAGFHGV-AGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYAL 234

Query: 253 GESDLPYSIRLAP-NGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPFLT 312
            ESDLPYS+ + P NGE+ TLGR DF G+L   MTAHPK DP T E FAFRY    PF+T
Sbjct: 235 CESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFIT 294

Query: 313 FFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGTD 372
           +F FD+ G+K +DVPI S+ +PS +HDFAIT++YA+F E Q+  NPM M+M G S VG D
Sbjct: 295 YFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLD 354

Query: 373 PSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDA--VVLLAPNILSVEHTLE 432
            + V R+G++PRYA DES M+WF+VP FN++H  N W+E D   +VL+APNILS+EH L 
Sbjct: 355 RTMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLG 414

Query: 433 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 492
            M+L+ A ++ VRI+L TG V+   LS  +L+FGVI+  Y+G+KNR+ Y GV  P+PKI 
Sbjct: 415 NMELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIK 474

Query: 493 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 552
           G+ KLD       DC V  R FG GC+ GEPFFVP   +        ED GYVV Y H+E
Sbjct: 475 GIRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVPDNIDGYG----NEDSGYVVCYTHEE 534

Query: 553 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDL 584
           ++GES F+VMDAKSP+L+I+A V+LP R+PYGFHG+FVK+ +L
Sbjct: 535 DTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVKQAEL 572

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 7.3e-120
Identity = 212/370 (57.30%), Postives = 274/370 (74.05%), Query Frame = 0


Query: 219 QYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAP-NGEIETLGRQDFDGNL-FMSM 278
           Q +P  GIGLANTS+ F   RL+AL E DLPY +RL+P +G+I T+GR   + N+   S 
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGR--IENNVSTKST 61

Query: 279 TAHPKSDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQS-DVPIFSMNRPSFLHDFAITKK 338
           TAHPK+DP TGETF+F YGP+ P++T+ R+D +G K   DVPIFS   PSF+HDFAIT+ 
Sbjct: 62  TAHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEH 121

Query: 339 YAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHA 398
           YAVF +IQI   P   I+ GR  +G D   V R+GL+PRYA  +S+M+WFDVPGFN++H 
Sbjct: 122 YAVFPDIQIVMKP-AEIVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHV 181

Query: 399 INAWDED--DAVVLLAPNILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDF 458
           +NAW+E+  + VV++APN+  +E+ ++R DL+H  +E  RI+LK+G V+R  LS  NLDF
Sbjct: 182 VNAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDF 241

Query: 459 GVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFF 518
           GVI+  Y G+K+R+ Y GVGDPMPKI GVVK+D     R +C+VA R FG GC+GGEPFF
Sbjct: 242 GVIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFF 301

Query: 519 VPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGF 578
           VP    SS ++  EEDDGYVVSY+HDE  GES F+VMDA+SP+LEI+A V LPRRVPYGF
Sbjct: 302 VP---ASSKKSGGEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGF 361

Query: 579 HGLFVKETDL 584
           HGLFV E +L
Sbjct: 362 HGLFVTEAEL 365

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 7.8e-106
Identity = 200/502 (39.84%), Postives = 297/502 (59.16%), Query Frame = 0


Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 152
           P+  + DP   +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H F
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFF 171

Query: 153 DGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAA 212
           DGDGM+H+++  +G A    R+ +T ++  ER  G PVFP      +G T   AR  +  
Sbjct: 172 DGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFY 231

Query: 213 GRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNL 272
            R   G  +PA+G G+AN  + +F  RL A+ E DLPY +++ PNG+++T+GR DFDG L
Sbjct: 232 ARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQL 291

Query: 273 FMSMTAHPKSDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFA 332
             +M AHPK DP++GE FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFA
Sbjct: 292 ESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFA 351

Query: 333 ITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFN 392
           IT+ + V  + Q+ F    MI  G SPV  D + V+R G++ +YA D S +KW D P   
Sbjct: 352 ITENFVVVPDQQVVFKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCF 411

Query: 393 LIHAINAWD--EDDAVVLLAPNILSVEHTLERMD-LVHALIEEVRIDLKTGIVTRRPLST 452
             H  NAW+  E D VV++   +   +      D  + +++ E+R++LKTG  TRRP+ +
Sbjct: 412 CFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 471

Query: 453 R-----NLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIFG 512
                 NL+ G++N + +G+K +F Y  + +P PK+SG  K+D++  E     V   ++G
Sbjct: 472 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYG 531

Query: 513 PGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVV 572
              YGGEP F+P E         EED+GY++ +VHDE + +S+  +++A S  LE+ A V
Sbjct: 532 DNRYGGEPLFLPGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATV 591

Query: 573 KLPRRVPYGFHGLFVKETDLNK 586
           KLP RVPYGFHG F+   DL K
Sbjct: 592 KLPSRVPYGFHGTFIGADDLAK 596

[Close]

BLAST of Cp4.1LG01g01120 vs. NCBI nr
Match: XP_023531023.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1177 bits (3046), Expect = 0.0
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
           TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL
Sbjct: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586

[Close]

BLAST of Cp4.1LG01g01120 vs. NCBI nr
Match: KAG6600436.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1163 bits (3009), Expect = 0.0
Identity = 576/586 (98.29%), Postives = 584/586 (99.66%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGG LLLSPPIST+RPPFS+AITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGKLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
           TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAIT+KYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITQKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRF+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKE+DLNKL
Sbjct: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNKL 586

[Close]

BLAST of Cp4.1LG01g01120 vs. NCBI nr
Match: XP_022943214.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 573/586 (97.78%), Postives = 581/586 (99.15%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGGNLLLSPPIST+RPPFS+AITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
           TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGD L
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDSL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYA+DESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRF+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           NSGESKFIVMDAKSPKLEIIAVVKLPRRV YGFHGLFVKE+DL KL
Sbjct: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVSYGFHGLFVKESDLRKL 586

[Close]

BLAST of Cp4.1LG01g01120 vs. NCBI nr
Match: XP_022979981.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 568/586 (96.93%), Postives = 579/586 (98.81%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGGNLLLSPPIS +RPPFS+AITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSL SSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG+AVLCSRYVKTYKY
Sbjct: 121 VIQGSLASSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGQAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
            LERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 ILERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+F+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           N GESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKE+DLN L
Sbjct: 541 NFGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNML 586

[Close]

BLAST of Cp4.1LG01g01120 vs. NCBI nr
Match: KAG7031088.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 544/586 (92.83%), Postives = 552/586 (94.20%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGG LLLSPPIST+RPPFS+AITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGKLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
           TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAIT+KYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITQKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHALIEE                                KNRF+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEE--------------------------------KNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKE+DLNKL
Sbjct: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNKL 554

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL
Match: A0A6J1FTM6 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448011 PE=3 SV=1)

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 573/586 (97.78%), Postives = 581/586 (99.15%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGGNLLLSPPIST+RPPFS+AITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
           TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGD L
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDSL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYA+DESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRF+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           NSGESKFIVMDAKSPKLEIIAVVKLPRRV YGFHGLFVKE+DL KL
Sbjct: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVSYGFHGLFVKESDLRKL 586

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL
Match: A0A6J1ISA3 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111479509 PE=3 SV=1)

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 568/586 (96.93%), Postives = 579/586 (98.81%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGGNLLLSPPIS +RPPFS+AITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180
           VIQGSL SSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG+AVLCSRYVKTYKY
Sbjct: 121 VIQGSLASSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGQAVLCSRYVKTYKY 180

Query: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
            LERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 ILERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+F+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 540

Query: 541 NSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           N GESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKE+DLN L
Sbjct: 541 NFGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNML 586

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1051 bits (2718), Expect = 0.0
Identity = 520/594 (87.54%), Postives = 551/594 (92.76%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKT----NADSPSP--- 60
           MDAI SPFL+GGNLLLS PIS +R   S AI SV  EETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R  PPPA+AKG ST R+EPS+PA  FNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240
           RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAF 300
           VAFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 VAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKMKWFDVPG NL+HAINAWDEDDAV+L+APNI
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNI 420

Query: 421 LSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKNRF+YAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGY 540
           GDPMPKISGVVKLDVSQ+ERRDCIVA+R+FGP CYGGEPFFVPRERE+SDETA EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           VVSYVHDENSG+S+F+VMDAKSP+LEIIA VKLPRRVPYGFHGLFVKE+DL+KL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL
Match: A0A346DA10 (Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE=2 SV=1)

HSP 1 Score: 1030 bits (2663), Expect = 0.0
Identity = 506/594 (85.19%), Postives = 543/594 (91.41%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKT----NADSPSP--- 60
           MDAI SPFL+GGNLLLSP IS +R   S AI SV  EETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R  PPPA+AKG ST R+EPS+PA  FNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240
           RYVKTYKYTLERDAGHPV PNVFSGFNGL ASAARGAV AGR+ TGQ+NP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAF 300
           +AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKMKWFDVPG NL+HAINAWDEDDAV+L+APN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420

Query: 421 LSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKNRF+YAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGY 540
           GDPMPKISGVVKLDVSQ+ERRDCIVA+R+FGP CYGGEPFFVPRERE+SDE A EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAAEEDDGY 540

Query: 541 VVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           VVSYVHDENSG+S+F+VMDAKSP+LEIIA VKLPRRVPYGFHGLFVKE+DL+KL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL
Match: A0A346DA08 (Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2 SV=1)

HSP 1 Score: 1029 bits (2661), Expect = 0.0
Identity = 509/594 (85.69%), Postives = 543/594 (91.41%), Query Frame = 0


Query: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKT----NADSPSP--- 60
           MDAI SPFL+GGNLLLSPP S  R   S AI SV TEETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSPPTSKYRTAVSTAICSVLTEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R   PPA+AKG ST R+EPS+PA  FNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATHPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240
           RYVKTYKYTLERDAGHPV PNVFSGFNGL ASAARGAV AGR+ TGQ+NPANGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPANGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAF 300
           +AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKMKWFDVPG NL+HAINAWDE+DAV+L+APNI
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEEDAVILVAPNI 420

Query: 421 LSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKNRF+YAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPVYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGY 540
           GDPMPKISGVVKLDVSQ+ERRDCIVA+R+FGP  YGGEPFFVPRERESSDETA EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNYYGGEPFFVPRERESSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586
           VVSYVHDENSG+S+F+VMDAKSP+LEIIA VKLPRRVPYGFHGLFVKE+DL+KL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of Cp4.1LG01g01120 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 794.7 bits (2051), Expect = 5.2e-230
Identity = 384/574 (66.90%), Postives = 461/574 (80.31%), Query Frame = 0


Query: 26  PFSVAITSVFTEETPKTVKKTNADSPSPRRPPPP--------AMAKGPSTTRLEPSLPAR 85
           P  + I S   EE       TN    + RR  P          +   P   R E +L   
Sbjct: 28  PTLLRINSAVVEERSPI---TNPSDNNDRRNKPKTLHNRTNHTLVSSPPKLRPEMTLATA 87

Query: 86  FFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTECEVIQGSLPSSLNGAYIR 145
            F   +D+IN FI+PP  PSVDP++VL+DNFAPV DELPPT+CE+I G+LP SLNGAYIR
Sbjct: 88  LFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGAYIR 147

Query: 146 NGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFS 205
           NGPNPQ+LPRGPYHLFDGDGMLH+++I NG+A LCSRYVKTYKY +E+  G PV PNVFS
Sbjct: 148 NGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPNVFS 207

Query: 206 GFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAP 265
           GFNG+TAS ARGA+ A RVLTGQYNP NGIGLANTS+AFF +RL+ALGESDLPY++RL  
Sbjct: 208 GFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVRLTE 267

Query: 266 NGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQSDV 325
           +G+IET+GR DFDG L MSMTAHPK+DP TGETFAFRYGP+PPFLT+FRFD  G KQ DV
Sbjct: 268 SGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQRDV 327

Query: 326 PIFSMNRPSFLHDFAITKKYAVFTEIQIG--FNPMLMIMEGRSPVGTDPSTVSRVGLIPR 385
           PIFSM  PSFLHDFAITK++A+F EIQ+G   N + +++EG SPVGTD     R+G+IP+
Sbjct: 328 PIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGVIPK 387

Query: 386 YANDESKMKWFDVPGFNLIHAINAWDEDD--AVVLLAPNILSVEHTLERMDLVHALIEEV 445
           YA DES+MKWF+VPGFN+IHAINAWDEDD  +VVL+APNI+S+EHTLERMDLVHAL+E+V
Sbjct: 388 YAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLERMDLVHALVEKV 447

Query: 446 RIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQER 505
           +IDL TGIV R P+S RNLDF VINP+++G+ +R++YA +GDPMPKISGVVKLDVS+ +R
Sbjct: 448 KIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSKGDR 507

Query: 506 RDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDA 565
            DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE +GESKF+VMDA
Sbjct: 508 DDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDEVTGESKFLVMDA 567

Query: 566 KSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 587
           KSP+LEI+A V+LPRRVPYGFHGLFVKE+DLNKL
Sbjct: 568 KSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

[Close]

BLAST of Cp4.1LG01g01120 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 386.0 bits (990), Expect = 5.6e-107
Identity = 200/502 (39.84%), Postives = 297/502 (59.16%), Query Frame = 0


Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 152
           P+  + DP   +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H F
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFF 171

Query: 153 DGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAA 212
           DGDGM+H+++  +G A    R+ +T ++  ER  G PVFP      +G T   AR  +  
Sbjct: 172 DGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFY 231

Query: 213 GRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNL 272
            R   G  +PA+G G+AN  + +F  RL A+ E DLPY +++ PNG+++T+GR DFDG L
Sbjct: 232 ARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQL 291

Query: 273 FMSMTAHPKSDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFA 332
             +M AHPK DP++GE FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFA
Sbjct: 292 ESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFA 351

Query: 333 ITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFN 392
           IT+ + V  + Q+ F    MI  G SPV  D + V+R G++ +YA D S +KW D P   
Sbjct: 352 ITENFVVVPDQQVVFKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCF 411

Query: 393 LIHAINAWD--EDDAVVLLAPNILSVEHTLERMD-LVHALIEEVRIDLKTGIVTRRPLST 452
             H  NAW+  E D VV++   +   +      D  + +++ E+R++LKTG  TRRP+ +
Sbjct: 412 CFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 471

Query: 453 R-----NLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIFG 512
                 NL+ G++N + +G+K +F Y  + +P PK+SG  K+D++  E     V   ++G
Sbjct: 472 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYG 531

Query: 513 PGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVV 572
              YGGEP F+P E         EED+GY++ +VHDE + +S+  +++A S  LE+ A V
Sbjct: 532 DNRYGGEPLFLPGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATV 591

Query: 573 KLPRRVPYGFHGLFVKETDLNK 586
           KLP RVPYGFHG F+   DL K
Sbjct: 592 KLPSRVPYGFHGTFIGADDLAK 596

[Close]

BLAST of Cp4.1LG01g01120 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 382.1 bits (980), Expect = 8.0e-106
Identity = 224/599 (37.40%), Postives = 329/599 (54.92%), Query Frame = 0


Query: 4   IFSPFLTGGNLLLSP-PISTARPPFSVAITSV----------FTEETPKTVKKTNADSPS 63
           I +P  T  NL  +P  +    P  SV+ T+              +TP  +   N  SP+
Sbjct: 6   ILTPNPTKLNLSFAPSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPN 65

Query: 64  PRRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 123
           P  P        P    L+ +  A    A   L+    + P+  +VDPR+ ++ N+APV 
Sbjct: 66  PIIPEKDTSRWNP----LQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVP 125

Query: 124 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 183
           E        + G +P  ++G Y+RNG NP + P   +HLFDGDGM+H+++I+NG A    
Sbjct: 126 EQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSC 185

Query: 184 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 243
           R+ +T +   E+  G P+FP      +G  +  AR  +   R L G  N  NG G+AN  
Sbjct: 186 RFTETERLVQEKQLGSPIFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAG 245

Query: 244 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAF 303
           + +F DRL A+ E DLPY +R+  NG++ET+GR DFDG L  +M AHPK DP T E FA 
Sbjct: 246 LVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFAL 305

Query: 304 RYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLM 363
            Y  +  P+L +F+F   G K  DV I  +  P+ +HDFAIT+ + V  + Q+ F    M
Sbjct: 306 SYDVVKKPYLKYFKFSPEGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDM 365

Query: 364 IMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWD--EDDAVVLLA 423
            + G+SPV  D   +SR G++PR A D S+M W + P     H  NAW+  E D VV++ 
Sbjct: 366 FL-GKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIG 425

Query: 424 PNILSVEHTLERMD-LVHALIEEVRIDLKTGIVTRRPL----STRNLDFGVINPSYIGKK 483
             +   +      D  +++++ E+R++LKTG  TRR +       NL+ G++N + +G+K
Sbjct: 426 SCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRK 485

Query: 484 NRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDET 543
            R+ Y  + +P PK+SG  K+D+S  E     V +  +G   YGGEPFF+PR  ES    
Sbjct: 486 TRYAYLAIAEPWPKVSGFAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-- 545

Query: 544 ALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDL 584
              EDDGY++S+VHDE S ES+  +++A +  LE+ A VKLP RVPYGFHG FV   D+
Sbjct: 546 ---EDDGYIMSFVHDEESWESELHIVNAVT--LELEATVKLPSRVPYGFHGTFVNSADM 585

[Close]

BLAST of Cp4.1LG01g01120 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-103
Identity = 206/542 (38.01%), Postives = 307/542 (56.64%), Query Frame = 0


Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTEC 120
           ++ G S   + P     F +   DL+   +   +  +  P + L+ NFAP+ DE PP + 
Sbjct: 5   LSDGSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKD 64

Query: 121 EVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYK 180
             + G LP  LNG ++R GPNP++     YH FDGDGM+H +RI +G+A   SRYVKT +
Sbjct: 65  LPVHGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSR 124

Query: 181 YTLERDAGHPVFPNV--FSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFG 240
              E   G   F  +    GF GL     +      ++L   Y    G G ANT++ +  
Sbjct: 125 LKQEEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTY----GNGTANTALVYHH 184

Query: 241 DRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPL 300
            +L AL E+D PY I++  +G+++TLG  D+D  L  S TAHPK DP TGE F F Y   
Sbjct: 185 GKLLALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHT 244

Query: 301 PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRS 360
           PP+LT+    K+G     VPI +++ P  +HDFAIT+ YA+F ++ + F P  M+ E + 
Sbjct: 245 PPYLTYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKM 304

Query: 361 PVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLA-----PNI 420
               DP+  +R G++PRYA DE  ++WF++P   + H  NAW+E+D VVL+      P++
Sbjct: 305 IYSFDPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDL 364

Query: 421 LSVEHTL-ERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAG 480
             V   + E+++     + E+R ++KTG  +++ LS   +DF  IN  Y GKK R++Y  
Sbjct: 365 DMVSGKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGT 424

Query: 481 VGDPMPKISGVVKLDV---SQQERRDCIVASRI-----FGPGCYGGEPFFVPRERESSDE 540
           + D + K++G++K D+   ++  +R   V   I      G G YG E  +VPRE      
Sbjct: 425 ILDSIAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE------ 484

Query: 541 TALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDL 586
              EEDDGY++ +VHDEN+G+S   V+DAK+   E +AVV+LP RVPYGFH LFV E  L
Sbjct: 485 -TAEEDDGYLIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQL 534

[Close]

BLAST of Cp4.1LG01g01120 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 362.1 bits (928), Expect = 8.6e-100
Identity = 196/503 (38.97%), Postives = 290/503 (57.65%), Query Frame = 0


Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 152
           P   + DP   +A NF PV E P      + G++P  + G Y+RNG NP + P   +HLF
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 153 DGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAA 212
           DGDGM+H++R  NG      R+ +T +   ER+ G PVFP      +G     A+  +  
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHG-HLGIAKLMLFN 291

Query: 213 GRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNL 272
            R L G  +P  G+G+AN  + +F   L A+ E DLPY +++   G++ET GR DFDG L
Sbjct: 292 TRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQL 351

Query: 273 FMSMTAHPKSDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFA 332
             +M AHPK DP+T E FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFA
Sbjct: 352 KSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFA 411

Query: 333 ITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFN 392
           IT+ + V  + Q+ F    MI  G SPV  D    SR G++ + A D S ++W +VP   
Sbjct: 412 ITENFVVIPDQQVVFRLPEMI-RGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCF 471

Query: 393 LIHAINAWDE---DDAVV----LLAPNILSVEHTLERMDLVHALIEEVRIDLKTGIVTRR 452
             H  N+W+E   D+ VV    +  P+ +  EH     + + +++ E+R++LKTG  TRR
Sbjct: 472 CFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHD----ETLQSVLSEIRLNLKTGESTRR 531

Query: 453 PLSTR--NLDFGVINPSYIGKKNRFIYAGVGDPMPKISGVVKLDVSQQERRDCIVASRIF 512
           P+ +   NL+ G++N + +G+K R+ Y  + +P PK+SG  K+D+S  E     +   I+
Sbjct: 532 PVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGE-----IRKYIY 591

Query: 513 GPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDENSGESKFIVMDAKSPKLEIIAV 572
           G G YGGEP F+P    S D    EED GY++ +VHDE   +S+  +++A + KLE  A 
Sbjct: 592 GEGKYGGEPLFLP----SGDG---EEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--AT 651

Query: 573 VKLPRRVPYGFHGLFVKETDLNK 586
           V LP RVPYGFHG F+ + DL+K
Sbjct: 652 VTLPSRVPYGFHGTFISKEDLSK 653

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g01120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49675	7.3e-229	66.90	Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thalian...	[more]
Q6YVJ0	1.9e-197	59.52	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q5MBR6	1.1e-165	56.41	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q84K96	7.3e-120	57.30	Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=8...	[more]
Q9LRR7	7.8e-106	39.84	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of Cp4.1LG01g01120 vs. NCBI nr


Match Name	Identity	Description
XP_023531023.1	100.00	probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp....	[more]
KAG6600436.1	98.29	putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar...	[more]
XP_022943214.1	97.78	probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata]	[more]
XP_022979981.1	96.93	probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima]	[more]
KAG7031088.1	92.83	putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar...	[more]

BLAST of Cp4.1LG01g01120 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1FTM6	97.78	probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata ...	[more]
A0A6J1ISA3	96.93	probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita maxima OX...	[more]
A0A346DA05	87.54	Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A346DA10	85.19	Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE...	[more]
A0A346DA08	85.69	Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2...	[more]

BLAST of Cp4.1LG01g01120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19170.1	5.2e-230	66.90	nine-cis-epoxycarotenoid dioxygenase 4	[more]
AT3G14440.1	5.6e-107	39.84	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT1G30100.1	8.0e-106	37.40	nine-cis-epoxycarotenoid dioxygenase 5	[more]
AT3G63520.1	1.3e-103	38.01	carotenoid cleavage dioxygenase 1	[more]
AT1G78390.1	8.6e-100	38.97	nine-cis-epoxycarotenoid dioxygenase 9	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 109..578 e-value: 6.9E-109 score: 365.1
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 30..585
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..70
None	No IPR available	PANTHER	PTHR10543:SF46	CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATED	coord: 30..585

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g01120.1	Cp4.1LG01g01120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen