Cp4.1LG01g00860 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g00860
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCaffeoyl-CoA O-methyltransferase
LocationCp4.1LG01: 3351653 .. 3355070 (-)
RNA-Seq ExpressionCp4.1LG01g00860
SyntenyCp4.1LG01g00860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTGGCCTGAAAGGGGATGAATTGGGGGGTCCTAGATATCGGGCAATGTGCCAGCGAGAAGGTTTAGCTCCGAATGGGATGGAGAACGAAATATTCTCTATAAAGCGTAGAAACTTCTCCTAGCAGATGGGTTCTAAAAATTTTGAGGGAGAGTTCGAACGGGAAAGTCGGACAATATCTACTCAGGGTGGAAACGGCTTTTTGGCTTGTTCTATCCTATTGTAATTAGTTGATTGAGGGAAACCTTGTGATTCTTCGTTTTTTTTTGTAGATCAGAACCAGAGCAAGATGCAACAAACGAGCTCCGAATCTTACCGGAGCTGGAACAGATAAGGAGACTAATGGCGATGAATCTAATGACTTGCCAATGTTTAATCAAACGACGCCGTGTGAACCTCTAGTGGCTATATCTGTAGCTGCGTGCCGTCTTCCCACCGGGCCGTAGTTCAGTGCGAGTCTTCAACTGGTTTCTTCTCAACAGGTACGTTTTCTTTCTGCGTTTTGATGTCTTCCCTCTTCGTTTAATCTGCAAAGCTTTAACATTCTCTCGCATGGCTTCGCGTCATCCCCGCATGCCCACCAGCTGTTTGTAGAAATGCCCAAGAGAGTATCTAATTTGTCGAGCTATAAAGTTAGTATACTATTTGGTTTTCTGTTTCCTACTTTTCTTCATGGCTTTCTGCTCCACCCTGCAGTGCATATCGTTCTTTGATGATGTCAAGCAGCAATATGGGGCTGCGTTTTTTGTCATACCCACAAAGGAATCTGCTTATGAAGCTCTCATCTATGCCCCCAGTGGCTGCAATTGCCAGCTTCGGGACTATCAAAAGGGCTATGGCTTGCAGGCGTGTTCGTTGTCATTTGAACTTGGATGATTCCAGCAGCAAGAAGAGGCAGCTGGAAAGGGTTTGTTCCGCCAATTCAAATGGGAAATTTAGCAATAAAGAGTTCATTAGTGTTGACTCACGGCTTTATGAGTATATCCTCTCCAATGTTCGAGAGCCTGAGGTACAATGTTGTTGCTCATCTATATGTTAAGTACTTTATGATCTGTCTGATTGTGATTGAATTCGTTGATTTAGATCTTGGGGCAACTTCGTGAGGAGACCGCCTCATTGCGTGGTAGCAAAATGCAGGTATCGCCTGATCAAGCACAACTTCTTGCAATGATTGTTCAAATTCTCGGTGCAAAGCGGTGCATCGAAGTGGGTGTTTACACTGTAAGCCCCTTCCTCCCATTCTTACTGCTTAATAACAAGGTTTTGTTTTGAAGAGTGCTATAACTTTAGGGCTAACCATAGTTTTCTATTCTCATTTAGGGATATTCATCTCTGGCCGTAGCGTTAGTCCTTCCAGAATCAGGGCGCTTAGTTGCCTGTGAAAGGGATGCCATATATCTAGATGTTGCTAGAAAATATTATGAACTTGCCGGTGTTTCTCACAAGGTAAGTACTTCTGGTTCTTCTTTCATCAAATGATTATGAACAGTGACATGAATCTTTTGAGAAATACTATCTTCCTATTTTTCTGCTGATAATCTGCAACTTTCATATTGTAATAGCTCCTACATGATTGCAACCAATGTATTCTGTTTGAGCTGTAAACTGTTTGTAGCAAATTTTTTGTTAGTTCTTGCTAGTATATTTCCTTTTACCCACATGATTGCAACCACGTTGATTGGAGAGGGAAACAAACGAAGCATTATTTATAAGGGTGGGGAAACCTCTCCCTAGCATATGCGTTTTAAAAACCTTGAGGGGAAAGCCCAAAGAGGACAATATCTGGTAACGGTGGGCTTGGGCTGTTACAAATGATATTAGAGCTAGACACCGGGCGGTGCGCCAGCGAGGACACTAAGCCCTGAAAGGGGGTGGACACCGGGCGGTGTGCTAGCGATGACGCTGAGCCCTGAAGGGGGGTGGATTGTGAGATCCCATATCAATTGAAAAGTGGAATAAAACATTCTTATAAGGGTGTGGAAACCTCTCCCTATCTGATGCATTTTAAAAACCTTGAGGGGAAACGTGAAAGGGAATGTCCAAGGAGGACAATATCGACTAGCGGTGGGCTTGGGATGATACTCTAACTAATTAACCGGGAGATTTGAGAAAGGCCATTCATTTAATCAGATTCCTCAAATTTTAGACCAGCCATACAGTATACCCTCTTATGGGTATACAAAGATCGATCAATTTCGTGTATTTATGTTCATAATTTTAAGCTATAACATGTTTCTGATGATCAAAACATGGTTTTGGACAATTTCTGGAAGTAGGTGGATGTGAGACATGGGCTTGCAGCAGATACACTCAAATCACTTATTGCAAATGGTGAAGCTGGCAGGTGAAGTGTTTTATGTTATATCATGTTTTGATTCAAAAACTTGTTTCGTTAGTTGATGGAATGATCTTATAATGCTTCTATTAGATTTTGTTATAACTATCCCATTATGTTCTCTCTTTTACCTTCTTGGCGATTTAACATGATATCAAAGTTAAAAGGGTGAGCTTTAAAACTTCTGTGATTTTACTTCCTCCGATGGCCTTTTTACAAAGATTGAATAGATAACATATTTCTGTGCACGTATAGTTATGATTTTGCGTTCATCGATGCCGAGAAGAGGATGAACGAGGAGTACTTCGAACTGCTACTGCAACTGGTAAGACGAAAAACAATTAGCTTCTGCTAAAAAATCTGTTTATGTGTAATGGTTTTGTCAGATAGCCTATCTTTTCAGTCCATGATGTTGAAGTAAGTTCTTCATCGCCTTCATTCAGGTGAGAGTTGGAGGTATAATTGTGATTGACAATGTCCTTTGGTATGGAAGAGTAGCTGACCCAACGGTGCGTTAGCTTCGACATCTCGTGACATTGTGCCTCCATTTTAGTGTAGTTAACTGATGATCATGGTCTTTGAGCAGGTAAATGACAAGCAGACTGATAGCATTAGGAGATTGAATAAAAAGATAATGGAAGACGATCGAGTCGGGATTAGTATAGTGAGCATTTGATCTATGTTCCCTCTGCTAGCCTCCTTGAACTATGATGATGGTACTAAACTTGGTTTATTTAGGTACCCATTGGCGATGGCATGACAATTTGCCAGAAGAGATGAATGGTGCCTGCTGAACTTGAGGGCGTAGCATTAGCAAAACAGTGCATAAATGAATTTGAGTTTACTTTTGTGGATAAATATTTTATTTTTCTTTTGTGAATGTATTTCTATAGGATGTTGGAATATAATGCCATGTGGTATGATCATATCTACTTAGGAAAATCCAAAAAAAAAAAAAAAAGAAAAAAAAAAAGAAAACACTTCAATCATTGATGCATAATCATCAAGCTATATAATACAATATGGTTAGTAGTAATTTTAATTTTATTTGATAACGTGAATTTAACTCAATATTAAATCTCT

mRNA sequence

CACTGGCCTGAAAGGGGATGAATTGGGGGGTCCTAGATATCGGGCAATGTGCCAGCGAGAAGGTTTAGCTCCGAATGGGATGGAGAACGAAATATTCTCTATAAAGCGTAGAAACTTCTCCTAGCAGATGGGTTCTAAAAATTTTGAGGGAGAGTTCGAACGGGAAAGTCGGACAATATCTACTCAGGGTGGAAACGGCTTTTTGGCTTGTTCTATCCTATTGTAATTAGTTGATTGAGGGAAACCTTGTGATTCTTCGTTTTTTTTTGTAGATCAGAACCAGAGCAAGATGCAACAAACGAGCTCCGAATCTTACCGGAGCTGGAACAGATAAGGAGACTAATGGCGATGAATCTAATGACTTGCCAATGTTTAATCAAACGACGCCGTGTGAACCTCTAGTGGCTATATCTGTAGCTGCGTGCCGTCTTCCCACCGGGCCGTAGTTCAGTGCGAGTCTTCAACTGGTTTCTTCTCAACAGTGCATATCGTTCTTTGATGATGTCAAGCAGCAATATGGGGCTGCGTTTTTTGTCATACCCACAAAGGAATCTGCTTATGAAGCTCTCATCTATGCCCCCAGTGGCTGCAATTGCCAGCTTCGGGACTATCAAAAGGGCTATGGCTTGCAGGCGTGTTCGTTGTCATTTGAACTTGGATGATTCCAGCAGCAAGAAGAGGCAGCTGGAAAGGGTTTGTTCCGCCAATTCAAATGGGAAATTTAGCAATAAAGAGTTCATTAGTGTTGACTCACGGCTTTATGAGTATATCCTCTCCAATGTTCGAGAGCCTGAGATCTTGGGGCAACTTCGTGAGGAGACCGCCTCATTGCGTGGTAGCAAAATGCAGGTATCGCCTGATCAAGCACAACTTCTTGCAATGATTGTTCAAATTCTCGGTGCAAAGCGGTGCATCGAAGTGGGTGTTTACACTGGATATTCATCTCTGGCCGTAGCGTTAGTCCTTCCAGAATCAGGGCGCTTAGTTGCCTGTGAAAGGGATGCCATATATCTAGATGTTGCTAGAAAATATTATGAACTTGCCGGTGTTTCTCACAAGGTGGATGTGAGACATGGGCTTGCAGCAGATACACTCAAATCACTTATTGCAAATGGTGAAGCTGGCAGTTATGATTTTGCGTTCATCGATGCCGAGAAGAGGATGAACGAGGAGTACTTCGAACTGCTACTGCAACTGGTGAGAGTTGGAGGTATAATTGTGATTGACAATGTCCTTTGGTATGGAAGAGTAGCTGACCCAACGGTAAATGACAAGCAGACTGATAGCATTAGGAGATTGAATAAAAAGATAATGGAAGACGATCGAGTCGGGATTAGTATAGTACCCATTGGCGATGGCATGACAATTTGCCAGAAGAGATGAATGGTGCCTGCTGAACTTGAGGGCGTAGCATTAGCAAAACAGTGCATAAATGAATTTGAGTTTACTTTTGTGGATAAATATTTTATTTTTCTTTTGTGAATGTATTTCTATAGGATGTTGGAATATAATGCCATGTGGTATGATCATATCTACTTAGGAAAATCCAAAAAAAAAAAAAAAAGAAAAAAAAAAAGAAAACACTTCAATCATTGATGCATAATCATCAAGCTATATAATACAATATGGTTAGTAGTAATTTTAATTTTATTTGATAACGTGAATTTAACTCAATATTAAATCTCT

Coding sequence (CDS)

ATGATGTCAAGCAGCAATATGGGGCTGCGTTTTTTGTCATACCCACAAAGGAATCTGCTTATGAAGCTCTCATCTATGCCCCCAGTGGCTGCAATTGCCAGCTTCGGGACTATCAAAAGGGCTATGGCTTGCAGGCGTGTTCGTTGTCATTTGAACTTGGATGATTCCAGCAGCAAGAAGAGGCAGCTGGAAAGGGTTTGTTCCGCCAATTCAAATGGGAAATTTAGCAATAAAGAGTTCATTAGTGTTGACTCACGGCTTTATGAGTATATCCTCTCCAATGTTCGAGAGCCTGAGATCTTGGGGCAACTTCGTGAGGAGACCGCCTCATTGCGTGGTAGCAAAATGCAGGTATCGCCTGATCAAGCACAACTTCTTGCAATGATTGTTCAAATTCTCGGTGCAAAGCGGTGCATCGAAGTGGGTGTTTACACTGGATATTCATCTCTGGCCGTAGCGTTAGTCCTTCCAGAATCAGGGCGCTTAGTTGCCTGTGAAAGGGATGCCATATATCTAGATGTTGCTAGAAAATATTATGAACTTGCCGGTGTTTCTCACAAGGTGGATGTGAGACATGGGCTTGCAGCAGATACACTCAAATCACTTATTGCAAATGGTGAAGCTGGCAGTTATGATTTTGCGTTCATCGATGCCGAGAAGAGGATGAACGAGGAGTACTTCGAACTGCTACTGCAACTGGTGAGAGTTGGAGGTATAATTGTGATTGACAATGTCCTTTGGTATGGAAGAGTAGCTGACCCAACGGTAAATGACAAGCAGACTGATAGCATTAGGAGATTGAATAAAAAGATAATGGAAGACGATCGAGTCGGGATTAGTATAGTACCCATTGGCGATGGCATGACAATTTGCCAGAAGAGATGA

Protein sequence

MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKKRQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR
Homology
BLAST of Cp4.1LG01g00860 vs. ExPASy Swiss-Prot
Match: Q9XGP7 (Tricin synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ROMT-15 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 8.0e-43
Identity = 104/239 (43.51%), Postives = 147/239 (61.51%), Query Frame = 0

Query: 68  SANSNGKFSNKEFISVDSRLYEYILSNV---REPEILGQLREETASLRGSKMQVSPDQAQ 127
           +A+S+   +NK  +  D+ LY+Y+L      REPE +  LR  T   +   MQ S D+AQ
Sbjct: 13  NAHSDIDSTNKTLLKSDA-LYKYVLDTTVLPREPECMRDLRLITDKHQWGFMQSSADEAQ 72

Query: 128 LLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGV 187
           LL M++++ GAKR IEVGV+TGYS LA AL LPE G++VA + D    ++ R + E AGV
Sbjct: 73  LLGMLLKMAGAKRTIEVGVFTGYSLLATALALPEDGKVVAIDPDRESYEIGRPFLEKAGV 132

Query: 188 SHKVDVRHGLAADTLKSLIAN----GEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 247
           +HKVD R G   + L  L+A     G   ++DFAF+DA+K    +Y E LLQLVRVGG I
Sbjct: 133 AHKVDFREGKGLEKLDELLAEEAAAGREAAFDFAFVDADKPNYVKYHEQLLQLVRVGGHI 192

Query: 248 VIDNVLWYGRVADP------TVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQK 294
           V DN LW G VA P       ++ + + +IR LN ++  D R+ +  + I DG+TIC++
Sbjct: 193 VYDNTLWAGTVALPPDTPLSDLDRRFSVAIRDLNSRLAADPRIDVCQLAIADGITICRR 250

BLAST of Cp4.1LG01g00860 vs. ExPASy Swiss-Prot
Match: Q86VU5 (Catechol O-methyltransferase domain-containing protein 1 OS=Homo sapiens OX=9606 GN=COMTD1 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-41
Identity = 90/211 (42.65%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 84  DSRLYEYILS-NVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQILGAKRCIEVG 143
           DSRL++Y+LS ++RE   L  LR  T         ++ +QAQLLA + +++ AK+ +++G
Sbjct: 51  DSRLWQYLLSRSMREHPALRSLRLLTLEQPQGDSMMTCEQAQLLANLARLIQAKKALDLG 110

Query: 144 VYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKSL 203
            +TGYS+LA+AL LP  GR+V CE DA   ++ R  +  A   HK+D+R   A +TL  L
Sbjct: 111 TFTGYSALALALALPADGRVVTCEVDAQPPELGRPLWRQAEAEHKIDLRLKPALETLDEL 170

Query: 204 IANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQTD 263
           +A GEAG++D A +DA+K     Y+E  LQL+R GGI+ +  VLW G+V  P   D   +
Sbjct: 171 LAAGEAGTFDVAVVDADKENCSAYYERCLQLLRPGGILAVLRVLWRGKVLQPPKGDVAAE 230

Query: 264 SIRRLNKKIMEDDRVGISIVPIGDGMTICQK 294
            +R LN++I  D RV IS++P+GDG+T+  K
Sbjct: 231 CVRNLNERIRRDVRVYISLLPLGDGLTLAFK 261

BLAST of Cp4.1LG01g00860 vs. ExPASy Swiss-Prot
Match: Q9XGD5 (Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays OX=4577 GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-40
Identity = 104/254 (40.94%), Postives = 144/254 (56.69%), Query Frame = 0

Query: 64  ERVCSANSNG-------KFSNKEFISVDSRLYEYILSN---VREPEILGQLREETASLRG 123
           E+  + N NG       +  +K  +  D  LY+YIL      REPE + +LRE TA    
Sbjct: 17  EQQANGNGNGEQKTRHSEVGHKSLLKSDD-LYQYILDTSVYPREPESMKELREITAKHPW 76

Query: 124 SKMQVSPDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLD 183
           + M  S D+ Q L M+++++GAK+ +E+GVYTGYS LA AL LPE G ++A       +D
Sbjct: 77  NLMTTSADEGQFLNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILA-------MD 136

Query: 184 VARKYYEL-------AGVSHKVDVRHGLAADTLKSLIANGEA-GSYDFAFIDAEKRMNEE 243
           + R+ YEL       AGV HK+D R G A   L  L+A+ E  GS+DFAF+DA+K     
Sbjct: 137 INRENYELGLPCINKAGVGHKIDFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLN 196

Query: 244 YFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQ------TDSIRRLNKKIMEDDRVGI 294
           Y E LL+LVR GG+I  DN LW G V  P     +       D +  LN  +  DDRV I
Sbjct: 197 YHERLLKLVRPGGLIGYDNTLWNGSVVLPDDAPMRKYIRFYRDFVLALNSALAADDRVEI 256

BLAST of Cp4.1LG01g00860 vs. ExPASy Swiss-Prot
Match: Q00719 (O-methyltransferase MdmC OS=Streptomyces mycarofaciens OX=1949 GN=mdmC PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-40
Identity = 79/201 (39.30%), Postives = 131/201 (65.17%), Query Frame = 0

Query: 95  VREPEILGQLREETASLRGSK-MQVSPDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVA 154
           +RE  +L +L + TA L G + MQ+ P++AQ L ++++++GA+R +E+G +TGYS+L +A
Sbjct: 20  LREDGLLRELHDMTAQLPGGRAMQIMPEEAQFLGLLIRLVGARRVLEIGTFTGYSTLCMA 79

Query: 155 LVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKSLIANGEAGSYDF 214
             LP  GR+V C+    +  +   +++ AGV   +D+R G AA TL  L      G++D 
Sbjct: 80  RALPAGGRIVTCDISDKWPGIGAPFWQRAGVDGLIDLRIGDAARTLAELRERDGDGAFDL 139

Query: 215 AFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQTDSIRRLNKKIME 274
            F+DA+K     Y+E  L LVR GG++ IDN L++GRVADP  +D  T ++R LN  + +
Sbjct: 140 VFVDADKAGYLHYYEQALALVRPGGLVAIDNTLFFGRVADPAADDPDTVAVRTLNDLLRD 199

Query: 275 DDRVGISIVPIGDGMTICQKR 295
           D+RV I+++ + DG+T+ ++R
Sbjct: 200 DERVDIALLTVADGITLARRR 220

BLAST of Cp4.1LG01g00860 vs. ExPASy Swiss-Prot
Match: Q9XGD6 (Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays OX=4577 GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.2e-39
Identity = 103/249 (41.37%), Postives = 143/249 (57.43%), Query Frame = 0

Query: 69  ANSNG-------KFSNKEFISVDSRLYEYILSN---VREPEILGQLREETASLRGSKMQV 128
           AN NG       +  +K  +  D  LY+YIL      REPE + +LRE TA    + M  
Sbjct: 16  ANGNGEQKTRHSEVGHKSLLKSDD-LYQYILDTSVYPREPESMKELREVTAKHPWNLMTT 75

Query: 129 SPDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKY 188
           S D+ Q L M+++++GAK+ +E+GVYTGYS LA AL LPE G ++A       +D+ R+ 
Sbjct: 76  SADEGQFLNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILA-------MDINREN 135

Query: 189 YEL-------AGVSHKVDVRHGLAADTLKSLIANGE-AGSYDFAFIDAEKRMNEEYFELL 248
           YEL       AGV+HK+D R G A   L  LIA  +  GS+DF F+DA+K     Y E L
Sbjct: 136 YELGLPCIEKAGVAHKIDFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERL 195

Query: 249 LQLVRVGGIIVIDNVLWYGRVADPTVNDKQ------TDSIRRLNKKIMEDDRVGISIVPI 294
           L+LV++GG+I  DN LW G V  P     +       D +  LNK +  DDRV I  +P+
Sbjct: 196 LKLVKLGGLIGYDNTLWNGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADDRVEICQLPV 255

BLAST of Cp4.1LG01g00860 vs. NCBI nr
Match: XP_023528625.1 (uncharacterized protein LOC111791484 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569 bits (1466), Expect = 3.03e-204
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE
Sbjct: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. NCBI nr
Match: XP_022982193.1 (uncharacterized protein LOC111481091 [Cucurbita maxima])

HSP 1 Score: 564 bits (1453), Expect = 2.90e-202
Identity = 291/294 (98.98%), Postives = 293/294 (99.66%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMP VAAIASFGTIKRAMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPSVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. NCBI nr
Match: KAG7031120.1 (Tricin synthase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 559 bits (1440), Expect = 2.78e-200
Identity = 289/294 (98.30%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIK AMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKSAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERV SANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVRSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADPTVNDKQTDSIRRLNK+IMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPTVNDKQTDSIRRLNKRIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. NCBI nr
Match: XP_022942615.1 (uncharacterized protein LOC111447599 [Cucurbita moschata])

HSP 1 Score: 557 bits (1436), Expect = 1.13e-199
Identity = 288/294 (97.96%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIK AMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKSAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERV SANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVRSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKY+E
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYFE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADPTVNDKQTDSIRRLNK+IMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPTVNDKQTDSIRRLNKRIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. NCBI nr
Match: KAG6600478.1 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521 bits (1341), Expect = 4.61e-176
Identity = 268/273 (98.17%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 22  KLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKKRQLERVCSANSNGKFSNKEFI 81
           KLSSMPPVAAIASFGTIK AMACRRVRCHLNLDDSSSKKRQLERV SANSNGKFSNKEFI
Sbjct: 667 KLSSMPPVAAIASFGTIKSAMACRRVRCHLNLDDSSSKKRQLERVRSANSNGKFSNKEFI 726

Query: 82  SVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQILGAKRCIEV 141
           SVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAM+VQILGAKRCIEV
Sbjct: 727 SVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMLVQILGAKRCIEV 786

Query: 142 GVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKS 201
           GVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKS
Sbjct: 787 GVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKS 846

Query: 202 LIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQT 261
           LIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYG+VADPTVNDKQT
Sbjct: 847 LIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGKVADPTVNDKQT 906

Query: 262 DSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           DSIRRLNK+IMEDDRVGISIVPIGDGMTICQKR
Sbjct: 907 DSIRRLNKRIMEDDRVGISIVPIGDGMTICQKR 939

BLAST of Cp4.1LG01g00860 vs. ExPASy TrEMBL
Match: A0A6J1J482 (uncharacterized protein LOC111481091 OS=Cucurbita maxima OX=3661 GN=LOC111481091 PE=3 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 1.41e-202
Identity = 291/294 (98.98%), Postives = 293/294 (99.66%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMP VAAIASFGTIKRAMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPSVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. ExPASy TrEMBL
Match: A0A6J1FWM2 (uncharacterized protein LOC111447599 OS=Cucurbita moschata OX=3662 GN=LOC111447599 PE=3 SV=1)

HSP 1 Score: 557 bits (1436), Expect = 5.49e-200
Identity = 288/294 (97.96%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRRVRCHLNLDDSSSKK 60
           MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIK AMACRRVRCHLNLDDSSSKK
Sbjct: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKSAMACRRVRCHLNLDDSSSKK 60

Query: 61  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120
           RQLERV SANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP
Sbjct: 61  RQLERVRSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 120

Query: 121 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 180
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKY+E
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYFE 180

Query: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240
           LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 240

Query: 241 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADPTVNDKQTDSIRRLNK+IMEDDRVGISIVPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPTVNDKQTDSIRRLNKRIMEDDRVGISIVPIGDGMTICQKR 294

BLAST of Cp4.1LG01g00860 vs. ExPASy TrEMBL
Match: A0A1S3BSA9 (O-methyltransferase MdmC-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492978 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 1.87e-170
Identity = 250/295 (84.75%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRR-VRCHLNLDDSSSK 60
           +MSSSNMGLRF+SYPQ+NLL   SS+ PV A AS  T+K A +C   VRC  NLDD SSK
Sbjct: 37  LMSSSNMGLRFMSYPQKNLLTVFSSLSPVTAFASIKTLKGAFSCSYCVRCQFNLDDFSSK 96

Query: 61  KRQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVS 120
           KR LERV + NSNGKFSNK+FISVD +LYEYILSNVREPEILGQLREETAS+RGSKMQVS
Sbjct: 97  KRHLERVYTTNSNGKFSNKQFISVDPQLYEYILSNVREPEILGQLREETASMRGSKMQVS 156

Query: 121 PDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYY 180
           PDQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERD  YL+VA++YY
Sbjct: 157 PDQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDLEYLEVAKRYY 216

Query: 181 ELAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGI 240
           ELAGVSHKVDVRHGLAA TLKSLIANGEA SYDFAFIDAEKRMNEEYFELLLQLVRVGG+
Sbjct: 217 ELAGVSHKVDVRHGLAAYTLKSLIANGEASSYDFAFIDAEKRMNEEYFELLLQLVRVGGL 276

Query: 241 IVIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           IVIDNVLWYG+VADP V+DK+TDSIRRLNKKI+EDDRVGIS+VPIGDGMTICQKR
Sbjct: 277 IVIDNVLWYGKVADPMVDDKKTDSIRRLNKKILEDDRVGISMVPIGDGMTICQKR 331

BLAST of Cp4.1LG01g00860 vs. ExPASy TrEMBL
Match: A0A0A0L0A0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G064680 PE=3 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 8.30e-165
Identity = 246/294 (83.67%), Postives = 263/294 (89.46%), Query Frame = 0

Query: 2   MSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRR-VRCHLNLDDSSSKK 61
           M +SNMGLRF+SYPQ NLL K SS+ PV A  S  T+K A +C   V C  NLDD SSKK
Sbjct: 1   MLTSNMGLRFMSYPQ-NLLTKFSSLSPVTAFPSIKTVKGAFSCSCCVVCQFNLDDFSSKK 60

Query: 62  RQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSP 121
           R LERV S NSNGKFSNK+FISVDS LY+YILSNVREPEILGQLREETAS+R SKMQVSP
Sbjct: 61  RHLERVYSTNSNGKFSNKQFISVDSELYDYILSNVREPEILGQLREETASMRASKMQVSP 120

Query: 122 DQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYE 181
           DQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVAC+RD   L+VA++YYE
Sbjct: 121 DQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACDRDLECLEVAKRYYE 180

Query: 182 LAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGII 241
           LAGVSHKVDVRHGLAADTLKSLIANGEA SYDFAFIDAEKRMNEEYFELLLQLVRV G+I
Sbjct: 181 LAGVSHKVDVRHGLAADTLKSLIANGEASSYDFAFIDAEKRMNEEYFELLLQLVRVRGLI 240

Query: 242 VIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 294
           VIDNVLWYG+VADP V+DK TDSIRRLNKKIMEDDRVGIS+VPIGDGMTICQKR
Sbjct: 241 VIDNVLWYGKVADPMVDDKMTDSIRRLNKKIMEDDRVGISMVPIGDGMTICQKR 293

BLAST of Cp4.1LG01g00860 vs. ExPASy TrEMBL
Match: A0A1S3BT49 (O-methyltransferase MdmC-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103492978 PE=3 SV=1)

HSP 1 Score: 456 bits (1172), Expect = 1.30e-159
Identity = 237/281 (84.34%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 1   MMSSSNMGLRFLSYPQRNLLMKLSSMPPVAAIASFGTIKRAMACRR-VRCHLNLDDSSSK 60
           +MSSSNMGLRF+SYPQ+NLL   SS+ PV A AS  T+K A +C   VRC  NLDD SSK
Sbjct: 6   LMSSSNMGLRFMSYPQKNLLTVFSSLSPVTAFASIKTLKGAFSCSYCVRCQFNLDDFSSK 65

Query: 61  KRQLERVCSANSNGKFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVS 120
           KR LERV + NSNGKFSNK+FISVD +LYEYILSNVREPEILGQLREETAS+RGSKMQVS
Sbjct: 66  KRHLERVYTTNSNGKFSNKQFISVDPQLYEYILSNVREPEILGQLREETASMRGSKMQVS 125

Query: 121 PDQAQLLAMIVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYY 180
           PDQAQLLAM+VQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERD  YL+VA++YY
Sbjct: 126 PDQAQLLAMLVQILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDLEYLEVAKRYY 185

Query: 181 ELAGVSHKVDVRHGLAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGI 240
           ELAGVSHKVDVRHGLAA TLKSLIANGEA SYDFAFIDAEKRMNEEYFELLLQLVRVGG+
Sbjct: 186 ELAGVSHKVDVRHGLAAYTLKSLIANGEASSYDFAFIDAEKRMNEEYFELLLQLVRVGGL 245

Query: 241 IVIDNVLWYGRVADPTVNDKQTDSIRRLNKKIMEDDRVGIS 280
           IVIDNVLWYG+VADP V+DK+TDSIRRLNKKI+EDDRVGIS
Sbjct: 246 IVIDNVLWYGKVADPMVDDKKTDSIRRLNKKILEDDRVGIS 286

BLAST of Cp4.1LG01g00860 vs. TAIR 10
Match: AT3G62000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 337.8 bits (865), Expect = 8.7e-93
Identity = 165/221 (74.66%), Postives = 199/221 (90.05%), Query Frame = 0

Query: 74  KFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQIL 133
           K+ NK+ IS+  RLY+Y+LSNVREP+IL QLREET+ +RGS+MQVSPDQAQLLAM+VQ+L
Sbjct: 58  KYGNKQVISLTPRLYDYVLSNVREPKILRQLREETSKMRGSQMQVSPDQAQLLAMLVQML 117

Query: 134 GAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHG 193
            A+RCIEVGVYTGYSSLAVALVLPESG LVACERD+  L+VA++YYELAGVSHKV+V+ G
Sbjct: 118 AAERCIEVGVYTGYSSLAVALVLPESGCLVACERDSNSLEVAKRYYELAGVSHKVNVKQG 177

Query: 194 LAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVAD 253
           LAA++LKS+I NGE  SYDFAF+DA+KRM ++YFELLLQLVRVGG+IV+DNVLW+GRV+D
Sbjct: 178 LAAESLKSMIQNGEGASYDFAFVDADKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSD 237

Query: 254 PTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 295
           P VND +T SIR  NKK+M+D RV IS+VPIGDGMTIC+KR
Sbjct: 238 PMVNDAKTISIRNFNKKLMDDKRVSISMVPIGDGMTICRKR 278

BLAST of Cp4.1LG01g00860 vs. TAIR 10
Match: AT3G61990.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.2e-86
Identity = 161/221 (72.85%), Postives = 192/221 (86.88%), Query Frame = 0

Query: 74  KFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQIL 133
           K+ NK  IS+  RLY+Y+L+NVRE EIL QLREETA    S++QVSPDQAQLLAM+V+IL
Sbjct: 73  KYGNKMVISLTPRLYDYVLNNVREHEILKQLREETAI---SQIQVSPDQAQLLAMLVEIL 132

Query: 134 GAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHG 193
           GAKRCIEVGVYTGYSSLAVALVLPESGRLVAC++DA  L+VA++YYELAGVSHKV V+HG
Sbjct: 133 GAKRCIEVGVYTGYSSLAVALVLPESGRLVACDKDANALEVAKRYYELAGVSHKVTVKHG 192

Query: 194 LAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVAD 253
           LAA++L S+I NGE  SYDFAF+DA+K M +EYFE LL+LVRVGG+IVIDNVLW+G VAD
Sbjct: 193 LAAESLMSMIQNGEESSYDFAFLDADKAMYQEYFESLLRLVRVGGVIVIDNVLWHGWVAD 252

Query: 254 PTVNDKQTDSIRRLNKKIMEDDRVGISIVPIGDGMTICQKR 295
            TVND++T S+R  NKK+M+D RV IS+V IGDGMTIC+KR
Sbjct: 253 STVNDERTISLRNFNKKLMDDQRVSISMVSIGDGMTICRKR 290

BLAST of Cp4.1LG01g00860 vs. TAIR 10
Match: AT3G62000.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.0e-85
Identity = 154/209 (73.68%), Postives = 187/209 (89.47%), Query Frame = 0

Query: 74  KFSNKEFISVDSRLYEYILSNVREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQIL 133
           K+ NK+ IS+  RLY+Y+LSNVREP+IL QLREET+ +RGS+MQVSPDQAQLLAM+VQ+L
Sbjct: 58  KYGNKQVISLTPRLYDYVLSNVREPKILRQLREETSKMRGSQMQVSPDQAQLLAMLVQML 117

Query: 134 GAKRCIEVGVYTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHG 193
            A+RCIEVGVYTGYSSLAVALVLPESG LVACERD+  L+VA++YYELAGVSHKV+V+ G
Sbjct: 118 AAERCIEVGVYTGYSSLAVALVLPESGCLVACERDSNSLEVAKRYYELAGVSHKVNVKQG 177

Query: 194 LAADTLKSLIANGEAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVAD 253
           LAA++LKS+I NGE  SYDFAF+DA+KRM ++YFELLLQLVRVGG+IV+DNVLW+GRV+D
Sbjct: 178 LAAESLKSMIQNGEGASYDFAFVDADKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSD 237

Query: 254 PTVNDKQTDSIRRLNKKIMEDDRVGISIV 283
           P VND +T SIR  NKK+M+D RV IS+V
Sbjct: 238 PMVNDAKTISIRNFNKKLMDDKRVSISMV 266

BLAST of Cp4.1LG01g00860 vs. TAIR 10
Match: AT4G26220.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 3.2e-39
Identity = 91/219 (41.55%), Postives = 129/219 (58.90%), Query Frame = 0

Query: 87  LYEYILSN---VREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQILGAKRCIEVGV 146
           LY+YIL      REPE+L +LR  T +   + M  +PD  QL+ M++ ++ A++ IEVGV
Sbjct: 15  LYKYILETSVYPREPEVLRELRNITHNHPQAGMATAPDAGQLMGMLLNLVNARKTIEVGV 74

Query: 147 YTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYELAGVSHKVDVRHGLAADTLKSLI 206
           +TGYS L  AL LPE G+++A + +    ++     + AGV HK+D +   A   L  L+
Sbjct: 75  FTGYSLLLTALTLPEDGKVIAIDMNRDSYEIGLPVIKKAGVEHKIDFKESEALPALDELL 134

Query: 207 ANG-EAGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPTVNDKQTD 266
            N    G +DFAF+DA+K     Y E L++L++VGGIIV DN LW G VA+P   D  T 
Sbjct: 135 NNKVNEGGFDFAFVDADKLNYWNYHERLIRLIKVGGIIVYDNTLWGGSVAEP---DSSTP 194

Query: 267 SIR--------RLNKKIMEDDRVGISIVPIGDGMTICQK 294
             R         LNKK+  D RV IS   +GDG+TIC++
Sbjct: 195 EWRIEVKKATLELNKKLSADQRVQISQAALGDGITICRR 230

BLAST of Cp4.1LG01g00860 vs. TAIR 10
Match: AT4G34050.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.2e-39
Identity = 92/224 (41.07%), Postives = 133/224 (59.38%), Query Frame = 0

Query: 87  LYEYILSN---VREPEILGQLREETASLRGSKMQVSPDQAQLLAMIVQILGAKRCIEVGV 146
           LY+YIL      REPE + +LRE TA    + M  S D+ Q L M+++++ AK  +E+GV
Sbjct: 41  LYQYILETSVYPREPESMKELREVTAKHPWNIMTTSADEGQFLNMLIKLVNAKNTMEIGV 100

Query: 147 YTGYSSLAVALVLPESGRLVACERDAIYLDVARKYYEL-------AGVSHKVDVRHGLAA 206
           YTGYS LA AL LPE G+++A       +DV R+ YEL       AGV+HK+D R G A 
Sbjct: 101 YTGYSLLATALALPEDGKILA-------MDVNRENYELGLPIIEKAGVAHKIDFREGPAL 160

Query: 207 DTLKSLIANGE-AGSYDFAFIDAEKRMNEEYFELLLQLVRVGGIIVIDNVLWYGRVADPT 266
             L  ++A+ +  G+YDF F+DA+K     Y + L+ LV++GG+I  DN LW G V  P 
Sbjct: 161 PVLDEIVADEKNHGTYDFIFVDADKDNYINYHKRLIDLVKIGGVIGYDNTLWNGSVVAPP 220

Query: 267 VNDKQ------TDSIRRLNKKIMEDDRVGISIVPIGDGMTICQK 294
               +       D +  LNK +  D R+ I ++P+GDG+TIC++
Sbjct: 221 DAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITICRR 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XGP78.0e-4343.51Tricin synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ROMT-15 PE=1 SV=1[more]
Q86VU52.6e-4142.65Catechol O-methyltransferase domain-containing protein 1 OS=Homo sapiens OX=9606... [more]
Q9XGD58.3e-4040.94Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays OX=4577 GN=CCOAOMT2 PE=2 SV=1[more]
Q007198.3e-4039.30O-methyltransferase MdmC OS=Streptomyces mycarofaciens OX=1949 GN=mdmC PE=3 SV=1[more]
Q9XGD63.2e-3941.37Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays OX=4577 GN=CCOAOMT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023528625.13.03e-204100.00uncharacterized protein LOC111791484 [Cucurbita pepo subsp. pepo][more]
XP_022982193.12.90e-20298.98uncharacterized protein LOC111481091 [Cucurbita maxima][more]
KAG7031120.12.78e-20098.30Tricin synthase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022942615.11.13e-19997.96uncharacterized protein LOC111447599 [Cucurbita moschata][more]
KAG6600478.14.61e-17698.179-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
A0A6J1J4821.41e-20298.98uncharacterized protein LOC111481091 OS=Cucurbita maxima OX=3661 GN=LOC111481091... [more]
A0A6J1FWM25.49e-20097.96uncharacterized protein LOC111447599 OS=Cucurbita moschata OX=3662 GN=LOC1114475... [more]
A0A1S3BSA91.87e-17084.75O-methyltransferase MdmC-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492978... [more]
A0A0A0L0A08.30e-16583.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G064680 PE=3 SV=1[more]
A0A1S3BT491.30e-15984.34O-methyltransferase MdmC-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103492978... [more]
Match NameE-valueIdentityDescription
AT3G62000.18.7e-9374.66S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G61990.11.2e-8672.85S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G62000.23.0e-8573.68S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G26220.13.2e-3941.55S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G34050.14.2e-3941.07S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002935Class I-like SAM-dependent O-methyltransferasePFAMPF01596Methyltransf_3coord: 95..293
e-value: 3.1E-68
score: 229.1
IPR002935Class I-like SAM-dependent O-methyltransferasePROSITEPS51682SAM_OMT_Icoord: 77..294
score: 63.028542
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 68..294
e-value: 1.4E-73
score: 249.0
NoneNo IPR availablePANTHERPTHR10509:SF14CATECHOL O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1coord: 69..294
NoneNo IPR availablePANTHERPTHR10509O-METHYLTRANSFERASE-RELATEDcoord: 69..294
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 137..242
e-value: 6.52712E-5
score: 39.3355
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 88..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g00860.1Cp4.1LG01g00860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity