Cp4.1LG01g00340 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g00340
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionethylene-responsive transcription factor CRF2-like
LocationCp4.1LG01: 3664021 .. 3664905 (-)
RNA-Seq ExpressionCp4.1LG01g00340
SyntenyCp4.1LG01g00340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAATGGATTTGTTCTCCAGTCTTCTTGTACCTTCGGTTAAGTACTCTGAACATCGAAACCACACCAAGCACTTTTCTCCGCCATTAATGGCGCCTAAGCTCGTCAGGATCTCTGTAACTGACGAAGATGCCACTGATTCTTCTAGTGATGAAAAGGAAGAACAACACGTCTCCCGACGAGTCAAGAAGTTTATTACCGAGATTACGATCGAGGCCTTGGGGAGAAGAGACCGGAAGAAGTCCACCGCCGGGAAATCGAATTTACGGAAGCCGCTACAGCCGGGAACTAAGAAATTCCGCGGCGTGAGGCAACGGCCCTGGGGAAAATGGGCGGCTGAGATTAGAGATCCTTTGCGACGTGTACGCATCTGGTTAGGTACCTACAATACGGCTGAAGAAGCCGCTATGGTGTACGACAACGCTGCCATTCAACTCCGTGGACCCACCGCCCAAACTAACTTCAGTCCTCCGCCGGCAGTGAGCTCCAGCAATGTCTCCGTTGAGGAGACCAACGACAATCTCTCTTCTCCGACCTCCGTTCTTCGTTGCCCCTCGCCGTCGACGAATGAAAAATCCCCTGAAACTGCTTCAACCGGTAAAGAAACCCGCCGTGCCGACTCCTTCTGTTCGAACTGCGATTCGTTTTACCCCAACGATATCTTCGACTTCCAACCGCCGATTCCGTCACTATTCGGCGATACAGCTCTCAACGACGCCGTTTTGAGGGGCGACTACGGCAGTAGCATGTTTATTAACCCGGATGATGATTTCGGGTTCGAGTTAGGATTCGGGTTCAGACCCTCCACGTGGCATATGAGTGAAAATTCTTTCCAAGACATCAGCGACATATTCGGGTCGGATCCTCTCGTTGCGCTTTAA

mRNA sequence

ATGGAAGGAATGGATTTGTTCTCCAGTCTTCTTGTACCTTCGGTTAAGTACTCTGAACATCGAAACCACACCAAGCACTTTTCTCCGCCATTAATGGCGCCTAAGCTCGTCAGGATCTCTGTAACTGACGAAGATGCCACTGATTCTTCTAAAACCCGCCGTGCCGACTCCTTCTGTTCGAACTGCGATTCGTTTTACCCCAACGATATCTTCGACTTCCAACCGCCGATTCCGTCACTATTCGGCGATACAGCTCTCAACGACGCCGTTTTGAGGGGCGACTACGGCAGTAGCATGTTTATTAACCCGGATGATGATTTCGGGTTCGAGTTAGGATTCGGGTTCAGACCCTCCACGTGGCATATGAGTGAAAATTCTTTCCAAGACATCAGCGACATATTCGGGTCGGATCCTCTCGTTGCGCTTTAA

Coding sequence (CDS)

ATGGAAGGAATGGATTTGTTCTCCAGTCTTCTTGTACCTTCGGTTAAGTACTCTGAACATCGAAACCACACCAAGCACTTTTCTCCGCCATTAATGGCGCCTAAGCTCGTCAGGATCTCTGTAACTGACGAAGATGCCACTGATTCTTCTAAAACCCGCCGTGCCGACTCCTTCTGTTCGAACTGCGATTCGTTTTACCCCAACGATATCTTCGACTTCCAACCGCCGATTCCGTCACTATTCGGCGATACAGCTCTCAACGACGCCGTTTTGAGGGGCGACTACGGCAGTAGCATGTTTATTAACCCGGATGATGATTTCGGGTTCGAGTTAGGATTCGGGTTCAGACCCTCCACGTGGCATATGAGTGAAAATTCTTTCCAAGACATCAGCGACATATTCGGGTCGGATCCTCTCGTTGCGCTTTAA

Protein sequence

MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSKTRRADSFCSNCDSFYPNDIFDFQPPIPSLFGDTALNDAVLRGDYGSSMFINPDDDFGFELGFGFRPSTWHMSENSFQDISDIFGSDPLVAL
Homology
BLAST of Cp4.1LG01g00340 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 7.7e-07
Identity = 31/79 (39.24%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 65  FYPNDIFDFQPP-IPSLFGDTALNDAVLRGDYGSSMFINPDDDFGFELGFGFRPSTWHMS 124
           F  +D+FDF+   +P   G     + +   D  + M      +FGF+ G G   S+WHM 
Sbjct: 274 FSDDDVFDFRSSVVPDYLGGDLFGEDLFTADMCTDM------NFGFDFGSGL--SSWHM- 333

Query: 125 ENSFQDISDIFGSDPLVAL 143
           E+ FQDI D+FGSDPL+A+
Sbjct: 334 EDHFQDIGDLFGSDPLLAV 343

BLAST of Cp4.1LG01g00340 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 9.4e-05
Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 65  FYPNDIFDFQPPIPSLFGDTALNDAVLRGD-YGSSMFINPDDDFGFELGFGFRPSTWHMS 124
           F  +D+F F   +   FG          GD +G ++F +     GF  G G   S+WH+ 
Sbjct: 220 FSDDDVFGFSTSMSESFG----------GDLFGDNLFADMSFGSGFGFGSGSGFSSWHV- 279

Query: 125 ENSFQDISDIFGSDPLVAL 143
           E+ FQDI D+FGSDP++ +
Sbjct: 280 EDHFQDIGDLFGSDPVLTV 287

BLAST of Cp4.1LG01g00340 vs. NCBI nr
Match: XP_023512254.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235 bits (599), Expect = 5.93e-75
Identity = 141/294 (47.96%), Postives = 142/294 (48.30%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS---------- 60
           MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS          
Sbjct: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEQHVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RRVKKFITEITIEALGRRDRKKSTAGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDPL 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 RRVRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSPT 180

BLAST of Cp4.1LG01g00340 vs. NCBI nr
Match: XP_022942751.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita moschata] >KAG7031174.1 Ethylene-responsive transcription factor CRF2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 226 bits (575), Expect = 3.28e-71
Identity = 139/302 (46.03%), Postives = 142/302 (47.02%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS---------- 60
           MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS          
Sbjct: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEEQHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SRRVKKFITEITIEALGRRDRKKSTAGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDP 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 LRRVRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSP 180

BLAST of Cp4.1LG01g00340 vs. NCBI nr
Match: XP_022982125.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita maxima])

HSP 1 Score: 223 bits (569), Expect = 2.58e-70
Identity = 139/301 (46.18%), Postives = 140/301 (46.51%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSK--------- 60
           MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS          
Sbjct: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEQHVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RRVKKFITEITIEALGRRDRKKSTTGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDPL 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 RRVRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSPT 180

BLAST of Cp4.1LG01g00340 vs. NCBI nr
Match: KAG6600534.1 (Ethylene-responsive transcription factor CRF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219 bits (558), Expect = 1.13e-68
Identity = 136/299 (45.48%), Postives = 139/299 (46.49%), Query Frame = 0

Query: 4   MDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS------------- 63
           MDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS             
Sbjct: 1   MDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEEQHVSRR 60

Query: 64  ------------------------------------------------------------ 123
                                                                       
Sbjct: 61  VKKFITEITIEALGRRDRKKSTAGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDPLRR 120

Query: 124 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 VRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSPTSV 180

BLAST of Cp4.1LG01g00340 vs. NCBI nr
Match: XP_038904776.1 (ethylene-responsive transcription factor CRF1-like [Benincasa hispida])

HSP 1 Score: 144 bits (364), Expect = 3.10e-39
Identity = 106/321 (33.02%), Postives = 119/321 (37.07%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS---------- 60
           ME MDLFS+L+VP +KY+EHRN TK  SPPLM PK+VRISVTD DATDSS          
Sbjct: 1   MEQMDLFSNLVVPPIKYTEHRNQTKLVSPPLMGPKVVRISVTDADATDSSSDEEKEEYPC 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RRVKKFVNEITIEASSTGRSSRKKSTGGKSKFPTVNRGSLKQMPAGSRKFRGVRQRPWGK 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 WAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNFTPPPVKSSPETATAV 180

BLAST of Cp4.1LG01g00340 vs. ExPASy TrEMBL
Match: A0A6J1FPS6 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662 GN=LOC111447693 PE=4 SV=1)

HSP 1 Score: 226 bits (575), Expect = 1.59e-71
Identity = 139/302 (46.03%), Postives = 142/302 (47.02%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS---------- 60
           MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS          
Sbjct: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEEQHV 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SRRVKKFITEITIEALGRRDRKKSTAGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDP 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 LRRVRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSP 180

BLAST of Cp4.1LG01g00340 vs. ExPASy TrEMBL
Match: A0A6J1J1R4 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita maxima OX=3661 GN=LOC111481052 PE=4 SV=1)

HSP 1 Score: 223 bits (569), Expect = 1.25e-70
Identity = 139/301 (46.18%), Postives = 140/301 (46.51%), Query Frame = 0

Query: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSK--------- 60
           MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSS          
Sbjct: 1   MEGMDLFSSLLVPSVKYSEHRNHTKHFSPPLMAPKLVRISVTDEDATDSSSDEKEEQHVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RRVKKFITEITIEALGRRDRKKSTTGKSNLRKPLQPGTKKFRGVRQRPWGKWAAEIRDPL 120

Query: 121 ------------------------------------------------------------ 142
                                                                       
Sbjct: 121 RRVRIWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFSPPPAVSSSNVSVEETNDNLSSPT 180

BLAST of Cp4.1LG01g00340 vs. ExPASy TrEMBL
Match: A0A6J1ETA3 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662 GN=LOC111437533 PE=4 SV=1)

HSP 1 Score: 139 bits (349), Expect = 2.47e-37
Identity = 65/86 (75.58%), Postives = 74/86 (86.05%), Query Frame = 0

Query: 57  SFCSNCDSFYPNDIFDFQPPIPSLFGDTALNDAVLRGDYGSSMFINPDDDFGFELGFGFR 116
           SF SN D+F+PNDIFDF+PP+PSLFG+T LND  L+GDYGSSMFINP DDFGF  GFGF 
Sbjct: 234 SFHSNYDTFFPNDIFDFRPPVPSLFGETTLNDGFLKGDYGSSMFINPADDFGF--GFGFG 293

Query: 117 PSTWHMSENSFQDISDIFGSDPLVAL 142
            STWH  ++SFQDISDIFGSDPL+AL
Sbjct: 294 LSTWHTVKDSFQDISDIFGSDPLLAL 317

BLAST of Cp4.1LG01g00340 vs. ExPASy TrEMBL
Match: A0A5D3D045 (Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004170 PE=4 SV=1)

HSP 1 Score: 136 bits (343), Expect = 1.85e-36
Identity = 68/106 (64.15%), Postives = 77/106 (72.64%), Query Frame = 0

Query: 37  VRISVTDEDATDSSKTRRADSFCSNCDSFYPNDIFDFQPPIPSLFGDTALNDAVLRGDYG 96
           V  +   E   D S+     SF SNCD+F+PNDIFDFQPP+PS F D  LNDA+L+GDYG
Sbjct: 212 VSATTGKEIRGDESEKFSDFSFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYG 271

Query: 97  SSMFINPDDDFGFELGFGFRPSTWHMSENSFQDISDIFGSDPLVAL 142
           SSMFINP DDF  E GFGF  STWH  E+SF D SDIFGSDPL+AL
Sbjct: 272 SSMFINPGDDF--EFGFGFGLSTWHTVEDSFLDFSDIFGSDPLLAL 315

BLAST of Cp4.1LG01g00340 vs. ExPASy TrEMBL
Match: A0A5A7TRV9 (Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001690 PE=4 SV=1)

HSP 1 Score: 136 bits (343), Expect = 1.85e-36
Identity = 68/106 (64.15%), Postives = 77/106 (72.64%), Query Frame = 0

Query: 37  VRISVTDEDATDSSKTRRADSFCSNCDSFYPNDIFDFQPPIPSLFGDTALNDAVLRGDYG 96
           V  +   E   D S+     SF SNCD+F+PNDIFDFQPP+PS F D  LNDA+L+GDYG
Sbjct: 212 VSATTGKEIRGDESEKFSDFSFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYG 271

Query: 97  SSMFINPDDDFGFELGFGFRPSTWHMSENSFQDISDIFGSDPLVAL 142
           SSMFINP DDF  E GFGF  STWH  E+SF D SDIFGSDPL+AL
Sbjct: 272 SSMFINPGDDF--EFGFGFGLSTWHTVEDSFLDFSDIFGSDPLLAL 315

BLAST of Cp4.1LG01g00340 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 55.1 bits (131), Expect = 5.4e-08
Identity = 31/79 (39.24%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 65  FYPNDIFDFQPP-IPSLFGDTALNDAVLRGDYGSSMFINPDDDFGFELGFGFRPSTWHMS 124
           F  +D+FDF+   +P   G     + +   D  + M      +FGF+ G G   S+WHM 
Sbjct: 274 FSDDDVFDFRSSVVPDYLGGDLFGEDLFTADMCTDM------NFGFDFGSGL--SSWHM- 333

Query: 125 ENSFQDISDIFGSDPLVAL 143
           E+ FQDI D+FGSDPL+A+
Sbjct: 334 EDHFQDIGDLFGSDPLLAV 343

BLAST of Cp4.1LG01g00340 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 55.1 bits (131), Expect = 5.4e-08
Identity = 31/79 (39.24%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 65  FYPNDIFDFQPP-IPSLFGDTALNDAVLRGDYGSSMFINPDDDFGFELGFGFRPSTWHMS 124
           F  +D+FDF+   +P   G     + +   D  + M      +FGF+ G G   S+WHM 
Sbjct: 274 FSDDDVFDFRSSVVPDYLGGDLFGEDLFTADMCTDM------NFGFDFGSGL--SSWHM- 333

Query: 125 ENSFQDISDIFGSDPLVAL 143
           E+ FQDI D+FGSDPL+A+
Sbjct: 334 EDHFQDIGDLFGSDPLLAV 343

BLAST of Cp4.1LG01g00340 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 48.1 bits (113), Expect = 6.7e-06
Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 65  FYPNDIFDFQPPIPSLFGDTALNDAVLRGD-YGSSMFINPDDDFGFELGFGFRPSTWHMS 124
           F  +D+F F   +   FG          GD +G ++F +     GF  G G   S+WH+ 
Sbjct: 220 FSDDDVFGFSTSMSESFG----------GDLFGDNLFADMSFGSGFGFGSGSGFSSWHV- 279

Query: 125 ENSFQDISDIFGSDPLVAL 143
           E+ FQDI D+FGSDP++ +
Sbjct: 280 EDHFQDIGDLFGSDPVLTV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUQ27.7e-0739.24Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825039.4e-0535.44Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_023512254.15.93e-7547.96ethylene-responsive transcription factor CRF2-like [Cucurbita pepo subsp. pepo][more]
XP_022942751.13.28e-7146.03ethylene-responsive transcription factor CRF2-like [Cucurbita moschata] >KAG7031... [more]
XP_022982125.12.58e-7046.18ethylene-responsive transcription factor CRF2-like [Cucurbita maxima][more]
KAG6600534.11.13e-6845.48Ethylene-responsive transcription factor CRF2, partial [Cucurbita argyrosperma s... [more]
XP_038904776.13.10e-3933.02ethylene-responsive transcription factor CRF1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FPS61.59e-7146.03ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1J1R41.25e-7046.18ethylene-responsive transcription factor CRF2-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1ETA32.47e-3775.58ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662... [more]
A0A5D3D0451.85e-3664.15Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa O... [more]
A0A5A7TRV91.85e-3664.15Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT4G23750.15.4e-0839.24cytokinin response factor 2 [more]
AT4G23750.25.4e-0839.24cytokinin response factor 2 [more]
AT4G11140.16.7e-0635.44cytokinin response factor 1 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g00340.1Cp4.1LG01g00340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity