Cp4.1LG00g02810 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG00g02810 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 6.1e-296
Identity = 581/942 (61.68%), Postives = 683/942 (72.51%), Query Frame = 0

Query: 1   MAIETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS++A P KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
                  ++  K E +        DSEDD+PLS  L GN  P     V +    K  K++
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180

Query: 181 GD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSG 240
           GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG T  
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240

Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
           +++   G +  KRPL    + S+ Q S KK K+S S T +              G  LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300

Query: 301 --VKAKQ-------EVDDDDDHIPISQRMK-----------KVVAVSANKSLSSKKTVSK 360
             +KAKQ         DDDDD +PIS+R K           K  AV  N + S+ K  ++
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360

Query: 361 -VVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
            VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420

Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
           ++YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480

Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
           FTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540

Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
           RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600

Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
           NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660

Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
           YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE 
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720

Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
            V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ 
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780

Query: 781 SKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
           +KDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840

Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 886
           RAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 900

BLAST of Cp4.1LG00g02810 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 3.7e-293
Identity = 561/935 (60.00%), Postives = 681/935 (72.83%), Query Frame = 0

Query: 1   MAIETFGKAPM------FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDV 60
           MA E F K  +      ++++DED++P+VFKR         +S      N+ S    +  
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKR---------NSNTAATTNRPSPINNAMR 60

Query: 61  CSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQS 120
            SA G +       +S P  SP+ S       ++ SS   P + S  +  + +  +  + 
Sbjct: 61  NSAIGSTK------SSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120

Query: 121 KHLVKAAVKEESDRHPT---NESSDSEDDKPLSFRLKGNS-------NPPGKGLVPTVLK 180
              V A  +    + P+   ++  DSEDDKPLS RLK +S       +  G+G     ++
Sbjct: 121 DRSVVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQAVQ 180

Query: 181 NKVEKSSGDS--------DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL- 240
               +  G S        D+  P+SS    K++   S       ++K+PL++ + +NGL 
Sbjct: 181 KSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNGLK 240

Query: 241 --TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------- 300
             T G   Q P    +KRPL      SS Q S K+PKLS+ + P+               
Sbjct: 241 PKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQDAK 300

Query: 301 GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKKTVSKVVSSSM 360
           G  L + K         K++  D DDH+PI+ RMK     S NKS S+K + SK+++SS 
Sbjct: 301 GKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKS--DSSNNKSSSAKPSSSKMIASSS 360

Query: 361 KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKL 420
           +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++++GK 
Sbjct: 361 RTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGKP 420

Query: 421 VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDW 480
           VDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+W
Sbjct: 421 VDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYEW 480

Query: 481 YQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHP 540
           Y  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEHP
Sbjct: 481 YMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHP 540

Query: 541 KMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKY 600
           KMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFKY
Sbjct: 541 KMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKY 600

Query: 601 VFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLA 660
           VFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKLA
Sbjct: 601 VFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLA 660

Query: 661 LRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAIT 720
           LRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI 
Sbjct: 661 LRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIG 720

Query: 721 QFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGSKDGDVA 780
           QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ ++DGDV 
Sbjct: 721 QFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVP 780

Query: 781 EKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKP 840
           EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+ 
Sbjct: 781 EKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRT 840

Query: 841 PLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRIT 886
           PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+DPRIT
Sbjct: 841 PLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRIT 900

BLAST of Cp4.1LG00g02810 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 3.1e-239
Identity = 482/826 (58.35%), Postives = 580/826 (70.22%), Query Frame = 0

Query: 99  SPGTTLKVSDSTDSQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFR-----LKGNSNP 158
           +PG T+   ++++   K L     KEE      + S DS+DDKPLS +     L GNS+ 
Sbjct: 4   NPGITVIKQNASNVMEKTL-----KEEGQSR--SNSEDSDDDKPLSHKLSSGALNGNSHH 63

Query: 159 PGKG---LVPTVLKNKVEKSSGDSDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKV 218
              G     P+   +   +     +DE+ LSS+  + + +  S       DE KPL S  
Sbjct: 64  IRMGSNLSCPSPYTSPKPRIIKGPEDEMLLSSELQLNAGA--SNAESSDSDESKPLAS-- 123

Query: 219 HQNGLTSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQE 278
                    +L+ P    +KRPL      +S    SKK KLS+ S P   KL + + ++ 
Sbjct: 124 ---------ELEDPSSSLNKRPL--IQPKNSDPSPSKKAKLSEPSAPTNRKLKTAEQEEA 183

Query: 279 VDDDDDHIPISQRMKKVVAVSANKSLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKST 338
             DDD  I             +N++  S    SKV     +  +  S   V  SK +K  
Sbjct: 184 AADDDPSI-------------SNRNKKSTTPASKVSDKKKRPDVSASVNKVDFSKSLKVP 243

Query: 339 KLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDT 398
            +L    +GQ     LVHNGVIFPP Y+ HGVKM+Y+   VDLTPEQEEVATMFAVM +T
Sbjct: 244 LVLVKGRNGQ----PLVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLET 303

Query: 399 DYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALK 458
           +YM KPKF+ENF +DWRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+K
Sbjct: 304 EYMTKPKFRENFMSDWRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIK 363

Query: 459 EEKMK-QEEKYMWAILDGV-KEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPK-DITIN 518
           E K + ++EKYMWA++DG+ +EKVGNFRV    + +      K  ++K+ ++ + DITIN
Sbjct: 364 EGKDETRKEKYMWAVVDGMSREKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITIN 423

Query: 519 IGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEK 578
           IGKDAPIPE PIPGERWKE+RHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+  +EK
Sbjct: 424 IGKDAPIPEPPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREK 483

Query: 579 YEKARKLKDYIKNIR-NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVG 638
           YEK+RKLKDYI+ IR  AYTKDF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVG
Sbjct: 484 YEKSRKLKDYIEGIRAAAYTKDFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVG 543

Query: 639 CCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKL 698
           CCTLKVENV+   PN+L+F+FLGKDSI+Y+N VEVE  VF AI QF+SGK G DDLFD+L
Sbjct: 544 CCTLKVENVETKRPNILKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQL 603

Query: 699 DTSKLNAHLKDLMPGLTAKVFRTYNASITLDDM-----LNRGSKDGDVAEKIVIYQHANK 758
           DTSKLNAHLK+LMPGLTAKVFRTYNASITLD+M     L+R +K GDVAEK+V+YQHANK
Sbjct: 604 DTSKLNAHLKELMPGLTAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANK 663

Query: 759 EVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKDADGKQKR 818
           EVAIICNHQRTVSKSH AQM RLNEKI EL+ +LKEL+ DL R  KGKPPLK++DGK KR
Sbjct: 664 EVAIICNHQRTVSKSHSAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKR 723

Query: 819 NLTPE----------------------VLEKKISQTNAKIEKIERDMQTKEDLKTVALGT 878
           NL  +                       + KKI+QTN KIEK+ERD +TKEDLKTVALGT
Sbjct: 724 NLNLKRYKGKLLKLIQKLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGT 783

Query: 879 SKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 886
           SKINYLDPRITVAWCKRH+VPIEKIFNKSLLAKFAWAMDV   FRF
Sbjct: 784 SKINYLDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVVLIFRF 790

BLAST of Cp4.1LG00g02810 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 525.8 bits (1353), Expect = 9.5e-148
Identity = 344/859 (40.05%), Postives = 480/859 (55.88%), Query Frame = 0

Query: 107 SDSTDSQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNK 166
           SDS     +   +    +      ++E SDS ++ PLS  L                 NK
Sbjct: 6   SDSVSLSIRRRQRRGSSKRISMKESDEESDSSENHPLSESL-----------------NK 65

Query: 167 VEKSSGDSDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGV 226
             KS  D DD IP+  +   +++S+ +  N      KK   +KV  NG          G+
Sbjct: 66  KSKSESDEDD-IPIRKR---RASSKKNMSNS---SSKKR--AKVMGNG----------GL 125

Query: 227 ISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKA-KQEVDDDDDHIPISQRMK 286
            + K+     +     + +   PK    S   GSK  +  A K+E  D DD +P+     
Sbjct: 126 KNGKKTAVVKEEEDFNEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTDDSVPLR---- 185

Query: 287 KVVAVSANKSLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTS 346
              AVS       K  +    S++  ++    +   +  K+  S  +     D  +KWT+
Sbjct: 186 ---AVSTVSLTPYKSELPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTT 245

Query: 347 LVHNGVIFPPPYQ--THGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFW 406
           L HNGVIF PPY+     VK+IY G  V+L PE EEVA  +A M +TD+ + P F++NF+
Sbjct: 246 LEHNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFF 305

Query: 407 NDWRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKY 466
            D+ K+  +   NH I+    CDFT ++  ++ ++E+KK M  E+KKA+K++K ++EEKY
Sbjct: 306 RDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKY 365

Query: 467 MWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIP 526
            W ILDG KEKVGNFR+EPPGLFRGRG HPK G LKRR++P+ ITINIG+  P+PE P+P
Sbjct: 366 KWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLP 425

Query: 527 GERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKN 586
           G +W EV+HDNTVTWLA W++ IN    KYVFLAA SSLKGQSD +KYEK+RKLKDYI +
Sbjct: 426 GHQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDD 485

Query: 587 IRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPP 646
           IR  Y KD  S+    RQ   A YLID  ALRAGNEK +DEADTVGCC+L+ E+V   PP
Sbjct: 486 IRKGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPP 545

Query: 647 NLLEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLM 706
             + F+FLGKDSI+Y N VEV+  VFK +  F +  K   D +FD+L T+ LN +L  LM
Sbjct: 546 RTVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLM 605

Query: 707 PGLTAKVFRTYNASITLDDMLNRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGA 766
            GL+AKVFRTYNAS T+ + L +  K+  +A+KI+ Y  AN+ VAI+CNHQR+V+K+H  
Sbjct: 606 DGLSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDV 665

Query: 767 QMSRLNEKISEL---RDILKELKIDLD-RAKKGKPPL----------------------- 826
           QM R  E+I  L   R  L+++ ++L+ +  K KP L                       
Sbjct: 666 QMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELE 725

Query: 827 ------------------------------------------KDADGKQKR----NLTPE 886
                                                      DA+ K K+      + E
Sbjct: 726 KEKIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSME 785

BLAST of Cp4.1LG00g02810 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.4e-146
Identity = 348/880 (39.55%), Postives = 496/880 (56.36%), Query Frame = 0

Query: 67  RTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKAAVKEES 126
           + T  PK +P+         A+ +  +  P K P  +   SDS+DS S          +S
Sbjct: 60  KLTPTPKPAPVTPKPT----AAVNGKKPIPKKKPVDSSSDSDSSDSSS----------DS 119

Query: 127 DRHPTNESSDSEDDKPLSFRLKGNSNPPGK---GLVPTVLKNKVEKSSGDSDDEIPLSSK 186
           D    ++SSDS+ D   S     +S+ P K     +P      V K+S +S    P ++ 
Sbjct: 120 D----SDSSDSDSDSSSSDSSSSDSDVPKKKPISKIPNKPSGTVSKTSKNS----PKTTT 179

Query: 187 FHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHSSPQ 246
               +T+ V         + KP   KV     +S D        SS       DS S   
Sbjct: 180 TTTTTTTTV---------KSKP-APKVKPEQSSSDDSSSDSSDDSSD------DSSSDSS 239

Query: 247 FSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRM-----------KKVVAVSA 306
              +KPK   S  P   K   +  K+E        P+ + +           KKV    +
Sbjct: 240 SEEEKPKKKPSKKPAAVK-KEITVKKETTTTTKKTPVKKEVEVKKETKTTTTKKVKKEES 299

Query: 307 NKSLSSKKTVSKVVSSSMKKTLKKSKK-PVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGV 366
           +     +KT +K  +++ K+ LK+ ++   ++ + V          D   KWT+L+HNG 
Sbjct: 300 DDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGP 359

Query: 367 IFPPPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLL- 426
           +FPP Y  HG+K +Y GK+V LTP+QEEVAT +A   +TD+++K  FK+NF+ +++ LL 
Sbjct: 360 VFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLT 419

Query: 427 -GKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVK 486
             +  +++ +D CDF+ I+   +T+KE++K  + EEK A   EK K +EK+ +A +DG K
Sbjct: 420 SEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFK 479

Query: 487 EKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRH 546
           EKVGN+ +EPPGLF GRG+HPK G LK+RI P D+T+NIG+   IP  PIPGE+WKE+ H
Sbjct: 480 EKVGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVH 539

Query: 547 DNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDF 606
           +NTVTWLAFW + +N   FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR  Y K F
Sbjct: 540 NNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSF 599

Query: 607 TSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLG 666
             +   +RQ+AVA YLID LALR GNEK +D+ADTVGCC+L+VE++K    N +  +FLG
Sbjct: 600 NDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLG 659

Query: 667 KDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRT 726
           KDS++Y NTV++    +K +  F  GK  TD LFD L  ++LN HLK  M GL+AKVFRT
Sbjct: 660 KDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRT 719

Query: 727 YNASITLDDMLNR--GSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEK 786
           YNASITL   L++    +   + EKI+ Y   N+EVAI+CNHQ+   K+    MS+++EK
Sbjct: 720 YNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEK 779

Query: 787 ISELRDILKELKIDLDRAKKGKPP-------------------------LKDADGK---- 846
           I ELRD+      DL R K GK P                          KD D +    
Sbjct: 780 IQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDK 839

Query: 847 ----------QKRNLTPEV---LEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYL 886
                     +K    P+    +++KI++   +I+K E     K++LKTVALGTSKINYL
Sbjct: 840 EISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYL 893

BLAST of Cp4.1LG00g02810 vs. NCBI nr
Match: XP_023520897.1 (DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo] >XP_023520898.1 DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1723 bits (4462), Expect = 0.0
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL
Sbjct: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885

BLAST of Cp4.1LG00g02810 vs. NCBI nr
Match: XP_022940477.1 (DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940487.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940496.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata])

HSP 1 Score: 1701 bits (4404), Expect = 0.0
Identity = 875/885 (98.87%), Postives = 879/885 (99.32%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSS QAP VKSPGTTLKVSDSTDSQSKHLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           AVKEESDRHPTNE+SDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG TSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV A+SANKSLSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV-ALSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Cp4.1LG00g02810 vs. NCBI nr
Match: KAG6596930.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028410.1 DNA topoisomerase 1 alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1698 bits (4397), Expect = 0.0
Identity = 874/885 (98.76%), Postives = 878/885 (99.21%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSS QAP VKSPGTTL VSDSTDSQSKHLV A
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL
Sbjct: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV AVSANK LSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV-AVSANKPLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Cp4.1LG00g02810 vs. NCBI nr
Match: XP_023005283.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005284.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005285.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005286.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])

HSP 1 Score: 1676 bits (4340), Expect = 0.0
Identity = 864/885 (97.63%), Postives = 873/885 (98.64%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSS QA  VKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           AVKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKV AVSANKSLSSKK
Sbjct: 241 -PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKV-AVSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of Cp4.1LG00g02810 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1536 bits (3976), Expect = 0.0
Identity = 795/890 (89.33%), Postives = 830/890 (93.26%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MA+ETFGKAPMFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKH 120
           ANGQSSNAQ R TSLPKTSPM+  E+G+QKAS SS Q P VKS GTTLKVSDS DS+ K 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRP-EIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           L  AAVKEE+D  HPT ++SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGFDEKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKS 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK   AV  NKS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAV--NKS 300

Query: 301 LSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
             SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPP
Sbjct: 301 SGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+++KGK VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTW 540

Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
           LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPT
Sbjct: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
           LDDMLNRG+KDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG00g02810 vs. ExPASy TrEMBL
Match: A0A6J1FIM6 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111446061 PE=3 SV=1)

HSP 1 Score: 1701 bits (4404), Expect = 0.0
Identity = 875/885 (98.87%), Postives = 879/885 (99.32%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSS QAP VKSPGTTLKVSDSTDSQSKHLVKA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           AVKEESDRHPTNE+SDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG TSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV A+SANKSLSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV-ALSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Cp4.1LG00g02810 vs. ExPASy TrEMBL
Match: A0A6J1L1R3 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111498332 PE=3 SV=1)

HSP 1 Score: 1676 bits (4340), Expect = 0.0
Identity = 864/885 (97.63%), Postives = 873/885 (98.64%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60
           MA+ETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSS QA  VKSPGTTLKVSDSTDSQS+HLVKA
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           AVKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKV AVSANKSLSSKK
Sbjct: 241 -PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKV-AVSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of Cp4.1LG00g02810 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1533 bits (3969), Expect = 0.0
Identity = 795/890 (89.33%), Postives = 828/890 (93.03%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MA+ETFGKA MFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKH 120
            NGQSSNAQ R TSLPKTSPM+  E+G+QKAS SS Q P VKS GTTLKVSDS DS+ K 
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRP-EIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           LV AAVKEE+D  HP  E+SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGFDEKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKS 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK   AV  NKS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAV--NKS 300

Query: 301 LSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
             SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPP
Sbjct: 301 SGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+++KGK VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTW 540

Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
           LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPT
Sbjct: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
           LDDMLNRG+KDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDIL
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG00g02810 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1531 bits (3963), Expect = 0.0
Identity = 794/890 (89.21%), Postives = 828/890 (93.03%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MA+ETFGKAPMFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKH 120
           ANGQSSNAQ R TSLPKTSPM+  E+G+QKA+ SS Q P VKS GTTLKVSDS DS+ K 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRP-EIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVKAAVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           L  AAVKEE+D  HPT E+SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGF EKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKS 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK   AV  NKS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAV--NKS 300

Query: 301 LSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
             SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPP
Sbjct: 301 SGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+++KGK VDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTW 540

Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
           LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPT
Sbjct: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
           LDDMLNRG+KDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDIL
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG00g02810 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1506 bits (3899), Expect = 0.0
Identity = 785/891 (88.10%), Postives = 821/891 (92.14%), Query Frame = 0

Query: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MA+ETFGKAPMFD+DDEDNMPIVFKRSSASRQNQLDSE K    QRLNKL GRQGSD+CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSST-QAPPVKSPGTTLKVSDSTDSQSK 120
            NGQSSN Q R TSLPKTSPM+S E+G+QKA  SS  Q P  KS G +LK SDS DS+ K
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRS-EIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVKAAVKEES-DRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDS 180
           +LV AAVKEE+   +P+ E+SDSEDDKPLS RLKGNSN P KGLV T+ KNKV+KSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLP 240
           DDE+PLSSKF MK  S   G  Q GFDEKKPL+ KVHQNG TS DKLQKP V+SSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANK 300
           N DSHS  QFS+KKPKLSDSS+PL SKLVS+KAKQEVDDDDD IPISQR+KK  A   NK
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPAT-VNK 300

Query: 301 SLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFP 360
           S SSK TVSKVVSSS+KKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWT+LVHNGVIFP
Sbjct: 301 SSSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFP 360

Query: 361 PPYQTHGVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNH 420
           PPYQTHGVKM+YKGK VDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNH
Sbjct: 361 PPYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNH 420

Query: 421 VIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGN 480
           VIQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGN
Sbjct: 421 VIQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGN 480

Query: 481 FRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVT 540
           FRVEPPGLFRGRGEHPKMGKLKRRI P DITINIGK APIPECPIPGERWKEVRHDNTVT
Sbjct: 481 FRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVT 540

Query: 541 WLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDP 600
           WLAFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRNAYTKDFTSKDP
Sbjct: 541 WLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDP 600

Query: 601 TRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIK 660
           TRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+
Sbjct: 601 TRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIR 660

Query: 661 YENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASI 720
           YENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASI
Sbjct: 661 YENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASI 720

Query: 721 TLDDMLNRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDI 780
           TLDDMLNRG+KDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDI
Sbjct: 721 TLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI 780

Query: 781 LKELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKT 840
           LKELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKT
Sbjct: 781 LKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKT 840

Query: 841 VALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           VALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 VALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Cp4.1LG00g02810 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1018.1 bits (2631), Expect = 4.4e-297
Identity = 581/942 (61.68%), Postives = 683/942 (72.51%), Query Frame = 0

Query: 1   MAIETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS++A P KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
                  ++  K E +        DSEDD+PLS  L GN  P     V +    K  K++
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180

Query: 181 GD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSG 240
           GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG T  
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240

Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
           +++   G +  KRPL    + S+ Q S KK K+S S T +              G  LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300

Query: 301 --VKAKQ-------EVDDDDDHIPISQRMK-----------KVVAVSANKSLSSKKTVSK 360
             +KAKQ         DDDDD +PIS+R K           K  AV  N + S+ K  ++
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360

Query: 361 -VVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
            VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420

Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
           ++YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480

Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
           FTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540

Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
           RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600

Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
           NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660

Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
           YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE 
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720

Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
            V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ 
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780

Query: 781 SKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
           +KDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840

Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 886
           RAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 900

BLAST of Cp4.1LG00g02810 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1013.1 bits (2618), Expect = 1.4e-295
Identity = 579/939 (61.66%), Postives = 680/939 (72.42%), Query Frame = 0

Query: 1   MAIETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS++A P KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
                  ++  K E +        DSEDD+PLS  L GN  P     V +    K  K++
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180

Query: 181 GD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSG 240
           GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG T  
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240

Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSKLVS 300
           +++   G +  KRPL    + S+ Q S KK K+S S T +              G  LVS
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300

Query: 301 --VKAKQ-------EVDDDDDHIPISQRMK-----------KVVAVSANKSLSSKKTVSK 360
             +KAKQ         DDDDD +PIS+R K           K  AV  N + S+ K  ++
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360

Query: 361 -VVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVK 420
            VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420

Query: 421 MIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
           ++YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480

Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
           FTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPPGLF
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLF 540

Query: 541 RGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
           RGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW DPI
Sbjct: 541 RGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPI 600

Query: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVAT 660
           NPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVAT
Sbjct: 601 NPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVAT 660

Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVEL 720
           YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE 
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEP 720

Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
            V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ 
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQE 780

Query: 781 SKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLD 840
           +KDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LD
Sbjct: 781 TKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLD 840

Query: 841 RAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKIN 883
           RAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKIN
Sbjct: 841 RAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKIN 900

BLAST of Cp4.1LG00g02810 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1008.8 bits (2607), Expect = 2.6e-294
Identity = 561/935 (60.00%), Postives = 681/935 (72.83%), Query Frame = 0

Query: 1   MAIETFGKAPM------FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDV 60
           MA E F K  +      ++++DED++P+VFKR         +S      N+ S    +  
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKR---------NSNTAATTNRPSPINNAMR 60

Query: 61  CSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQS 120
            SA G +       +S P  SP+ S       ++ SS   P + S  +  + +  +  + 
Sbjct: 61  NSAIGSTK------SSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120

Query: 121 KHLVKAAVKEESDRHPT---NESSDSEDDKPLSFRLKGNS-------NPPGKGLVPTVLK 180
              V A  +    + P+   ++  DSEDDKPLS RLK +S       +  G+G     ++
Sbjct: 121 DRSVVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQAVQ 180

Query: 181 NKVEKSSGDS--------DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL- 240
               +  G S        D+  P+SS    K++   S       ++K+PL++ + +NGL 
Sbjct: 181 KSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNGLK 240

Query: 241 --TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------- 300
             T G   Q P    +KRPL      SS Q S K+PKLS+ + P+               
Sbjct: 241 PKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQDAK 300

Query: 301 GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKKTVSKVVSSSM 360
           G  L + K         K++  D DDH+PI+ RMK     S NKS S+K + SK+++SS 
Sbjct: 301 GKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKS--DSSNNKSSSAKPSSSKMIASSS 360

Query: 361 KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKMIYKGKL 420
           +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVK++++GK 
Sbjct: 361 RTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGKP 420

Query: 421 VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDW 480
           VDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+W
Sbjct: 421 VDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYEW 480

Query: 481 YQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHP 540
           Y  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEHP
Sbjct: 481 YMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHP 540

Query: 541 KMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKY 600
           KMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFKY
Sbjct: 541 KMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKY 600

Query: 601 VFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLA 660
           VFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKLA
Sbjct: 601 VFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLA 660

Query: 661 LRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAIT 720
           LRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI 
Sbjct: 661 LRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIG 720

Query: 721 QFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGSKDGDVA 780
           QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ ++DGDV 
Sbjct: 721 QFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVP 780

Query: 781 EKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKP 840
           EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+ 
Sbjct: 781 EKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRT 840

Query: 841 PLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRIT 886
           PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+DPRIT
Sbjct: 841 PLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRIT 900

BLAST of Cp4.1LG00g02810 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 425.6 bits (1093), Expect = 9.5e-119
Identity = 207/271 (76.38%), Postives = 244/271 (90.04%), Query Frame = 0

Query: 615 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 674
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 675 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGSKDGDVAEKIV 734
           GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ +KDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 735 IYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKD 794
           +YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ 
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 795 ADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWC 854
           +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 855 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 886
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 93.2 bits (230), Expect = 1.1e-18
Identity = 111/307 (36.16%), Postives = 150/307 (48.86%), Query Frame = 0

Query: 1   MAIETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSANGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTD 120
           S V S NG        TT   KTS +KSS        +SS++A P KSP      S   D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 SQSKHLVKAAVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSS 180
                  ++  K E +        DSEDD+PLS  L GN  P     V +    K  K++
Sbjct: 121 RSQLQKDQSECKIEHE--------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK--KNN 180

Query: 181 GD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSG 240
           GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG T  
Sbjct: 181 GDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240

Query: 241 DKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHI 286
           +++   G +  KRPL    + S+ Q S KK K+S S T +  K  SV  K+E+ DD   +
Sbjct: 241 NEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSV--KKEI-DDKGRV 275

BLAST of Cp4.1LG00g02810 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 131.0 bits (328), Expect = 4.9e-30
Identity = 61/68 (89.71%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 818 KIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 877
           KIEK+ERDMQTKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 878 DVDPDFRF 886
           DV+P F F
Sbjct: 62  DVEPHFTF 69