Cp4.1LG00g00920 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG00g00920
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWAT1-related protein
LocationCp4.1LG00: 2081950 .. 2086388 (+)
RNA-Seq ExpressionCp4.1LG00g00920
SyntenyCp4.1LG00g00920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTCTAATCGGCAAACACTGCAACCACTGCAAGCACCTTCGGCGTGTCAATGGCGAAGCTGTATGCGTCGTGTGCAGCAATGGTGGCGGTGCAAATTGGGTATGGCGGATCCAACATTCTTATGAAAGTGGCTAGCGAGAAGGATCTTGATCCTTTCGTGTTCTTCGTGTATCGCCATATCATCGCCTTCCTCGTCTTGCTTCCTTTCGCTTATGTGATCGATAGGTGCAATAATAAAAGTCGTTTGTGTTCTTCCTTCGATTCATGGTTGATTATATGGTGATGATTGTTTGAATCATGCAGAAACAAACGGACTTCTTTGACGATTTCCATGACCATTAAGATCTTCTTGCTCGCCATTTTAGGCTCAACTATTTACTTGAATCTTTTCTACCTTGGAATTAAGTATACTTCAGCCACCGTCGCTTCCGCTTTCAGTAATGTCACTCCCAGCTTCACCTTCGTCATGGCTGTTTTGTTCAGGTACTCAAATAGAATTCATAACTCAACTCAATCCAATTCAACCGTCTATTGAGTGTTCCAGTTGTTTCCATTGTCAACTTCTCCCTGCCAATAGTTATATAGTGTGAGCAGTTGTTGTTACTCTCTTCCTTGCATATATATAACGTCTCTTTCAAAATCTCTCTTTCAAAAATCTCTCTCTGCCCTTATTTTCTAAAATCGAAATTAGAGGCTCGAGCATACGTCGTTTGGCCGTAGGCGACGTAAGAACGAATTGGCTTAGCTCACTTGTCGTTGGAAAGTGAGTACCGCACAATCGCAAGTTCGGTGCTATCAAGAGTGCGATGGTGACAATTACTCTCCATATTACATTAATAACTAAAAAAATTTAAAATTTAAATATCAAATATTCATTTTTATTGTTTAGGTTAGTTAGTTTAAGTTATCGGGTCATTTAAACACTCCTATGATCAGGTCGGTTGTTTTACATTATTTTTTTTTTTTAATTTTAATTTTGGGAAACTTTTTCCCAACTTTTAAAATGTGGGTGTAGTTGTACTAAAATTCCATTTTTACACACATTTTTGTCTCATTTTTTTCATTTTGTCGATAAATATGAATCATTCAAAAGTACTTCTTTACTAGTTTTGTAATTTATTATTATTATTATTATTTTAAAATTCACAACCATAGAAATTGGAACTTTTCAACTTTACAAAAGCTAAAATATCTCCCATCACTAAACAATATTAACATTGATCTAAAGGTGATGTATTTTCTTGAGGGAATCCTTACTTTTAATTTGGATCGAATACATTTTCAAAAGTAATGCTTAAAGATATATATTCGAAGTCGAGGGCATAGATTTTTTATGCCTAAAATTACCATACATTTATGTCAGTTGTGGATGTGACATACTTTTACCGAATAATATTTATTTCTTCATTTATGTTTATAATTCTCGATAATATTTAAATAATATTTTTATAAGAGTTCAAGTTAATAAATTATTAATTTAAGTATTTTTTAATTTTTTTATTTACTTATGTATAAAGATTGGAGAAAGTGAATATCAAAACTGCACGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCACTCATAAACATATACAAGAATGAGGACTCTGGGAATAGTAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGGTAACTTATGTTGAGGATTATTGGGATTGGGAGTGTTCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGACGAATACTATCTCCATTGGTATGAGGCCTTTTGGGAAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACAACTTAGTCTTATTTTATGCAACCAAATGAAATAGGCTCATCAAATCTCAATGCCTTGCAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGTGAGTGTATTGTCTCGTATAGGATACAAGCTATCAACTAACTTCTAAACAAACTATAACGGATGTGTGTAGGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTAATAACTCAACTCAATCCAATTCAACCGTCTATTGAGTGTTCCAGTTGTTTCCATTGTCAACTTCTCCCTGCCAATAGTTATATAGTGTGAGCAGTTGTTGTTACTCTCTTCCTTGCATATATATAACGTCTCTTTCAAAATCTCTCTTTCAAAAATCTCTCTCTGCCCTTATTTTCTAAAATCGAAATTAGAGGCTCGAGCATACGTCGTTTGGCCGTAGGCGACGTAAGAACGAATTGGCTTAGCTCACTTGTCGTTGGAAAGTGAGTACCGCACAATCGCAAGTTCGGTGCTATCAAGAGTGCGATGGTGACAATTACTCTCCATATTACATTAATAACTAAAAAAATTTAAAATTTAAATATCAAATATTCATTTTTATTGTTTAGGTTAGTTAGTTTAAGTTATCGGGTCATTTAAACACTCCTATGATCAGGTCGGTTGTTTTACATTATTTTTTTTTTTTAATTTTAATTTTGGGAAACTTTTTCCCAACTTTTAAAATGTGGGTGTAGTTGTACTAAAATTCCATTTTTACACACATTTTTGTCTCATTTTTTTCATTTTGTCGATAAATATGAATCATTCAAAAGTACTTCTTTACTAGTTTTGTAATTTATTATTATTATTATTATTTTAAAATTCACAACCATAGAAATTGGAACTTTTCAACTTTACAAAAGCTAAAATATCTCCCATCACTAAACAATATTAACATTGATCTAAAGGTGATGTATTTTCTTGAGGGAATCCTTACTTTTAATTTGGATCGAATACATTTTCAAAAGTAATGCTTAAAGATATATATTCGAAGTCGAGGGCATAGATTTTTTATGCCTAAAATTACCATACATTTATGTCAGTTGTGGATGTGACATACTTTTACCGAATAATATTTATTTCTTCATTTATGTTTATAATTCTCGATAATATTTAAATAATATTTTTATAAGAGTTCAAGTTAATAAATTATTAATTTAAGTATTTTTTAATTTTTTTATTTACTTATGTATAAAGATTGGAGAAAGTGAATATCAAAACTGCAAGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATCAGGACTCTGGGAATAGTAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGGTAACTTAGTCTTATTTTATGGAACCAAATGAAATAGGCTCATCAAATCTCAATGCCTTGCAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGTGAGTGTATTGTCTCGTATAGGATACAAGCTATCAACTAACTTCTAAACAAACTATAACGGATGTGTGTAGGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGAGGCTTCATGTGGGCAGGTAAGCCACTCCCTTCCTCTACCATGTTTGTATTGAATTGAAGGTGTTAACTGAAACTGTTGTGCTATGAAGCTTGGTGGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGACAATGAAAAGGGGTTGGTTGACACTGAAATGCAGAATACATCTGTAAATGATAATGAAGTGAAAGATAGTGTAAGAGAAACCAGAATTTCTCCGATTTGAGCTGCAAATGTTGCAAGTTAACTACGGAACACACCATGTGGCAGCACTTTGTTCTTTCACTGTTCTTGTATTGTATCTATACTTATATAAGTCTATATTTAGAATGGAGTTAGTTACTTGTAGAATTAA

mRNA sequence

GGTCTAATCGGCAAACACTGCAACCACTGCAAGCACCTTCGGCGTGTCAATGGCGAAGCTGTATGCGTCGTGTGCAGCAATGGTGGCGGTGCAAATTGGGATCTTGATCCTTTCGTGTTCTTCGTGTATCGCCATATCATCGCCTTCCTCGTCTTGCTTCCTTTCGCTTATGTGATCGATAGAAACAAACGGACTTCTTTGACGATTTCCATGACCATTAAGATCTTCTTGCTCGCCATTTTAGGCTCAACTATTTACTTGAATCTTTTCTACCTTGGAATTAAGTATACTTCAGCCACCGTCGCTTCCGCTTTCAGTAATGTCACTCCCAGCTTCACCTTCGTCATGGCTGTTTTGTTCAGATTGGAGAAAGTGAATATCAAAACTGCACGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCACTCATAAACATATACAAGAATGAGGACTCTGGGAATACTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGATACAAGCTATCAACTAACTTCTAAACAAACTATAACGGATGTGTGTAGGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAATTGGAGAAAGTGAATATCAAAACTGCAAGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATCAGGACTCTGGGAATAGTAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGAGGCTTCATGTGGGCAGCTTGGTGGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGACAATGAAAAGGGGTTGGTTGACACTGAAATGCAGAATACATCTGTAAATGATAATGAAGTGAAAGATAGTAATTAA

Coding sequence (CDS)

GGTCTAATCGGCAAACACTGCAACCACTGCAAGCACCTTCGGCGTGTCAATGGCGAAGCTGTATGCGTCGTGTGCAGCAATGGTGGCGGTGCAAATTGGGATCTTGATCCTTTCGTGTTCTTCGTGTATCGCCATATCATCGCCTTCCTCGTCTTGCTTCCTTTCGCTTATGTGATCGATAGAAACAAACGGACTTCTTTGACGATTTCCATGACCATTAAGATCTTCTTGCTCGCCATTTTAGGCTCAACTATTTACTTGAATCTTTTCTACCTTGGAATTAAGTATACTTCAGCCACCGTCGCTTCCGCTTTCAGTAATGTCACTCCCAGCTTCACCTTCGTCATGGCTGTTTTGTTCAGATTGGAGAAAGTGAATATCAAAACTGCACGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCACTCATAAACATATACAAGAATGAGGACTCTGGGAATACTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGATACAAGCTATCAACTAACTTCTAAACAAACTATAACGGATGTGTGTAGGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAATTGGAGAAAGTGAATATCAAAACTGCAAGAGGTGGTGCTAAAGTGTTGGGGACGGTTATCTGCATTGGTGGTTCGCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATCAGGACTCTGGGAATAGTAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTCGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATATAGTGGCATTATTATTTCGGCGGTCGGGTATTTCGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGAGGCTTCATGTGGGCAGCTTGGTGGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGACAATGAAAAGGGGTTGGTTGACACTGAAATGCAGAATACATCTGTAAATGATAATGAAGTGAAAGATAGTAATTAA

Protein sequence

GLIGKHCNHCKHLRRVNGEAVCVVCSNGGGANWDLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDSN
Homology
BLAST of Cp4.1LG00g00920 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.0e-46
Identity = 124/457 (27.13%), Postives = 203/457 (44.42%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           ++ ++   YRH++A +V+ PFA +++R  R  +T  + ++I  L  L   +  NL+Y+G+
Sbjct: 37  MNHWILATYRHVVATIVIAPFALILERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGM 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
           K TSAT +SAF N  P+ TF+MAV+FR+E VN+K  R  AKV+GT I +GG+++ T +KG
Sbjct: 97  KATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P +                                                         
Sbjct: 157 PAIE-------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                                      LF+ +  S   GSS T                 
Sbjct: 217 ---------------------------LFKTAHSSLHGGSSGT----------------- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                  S  ++ ++W+ G+  ++ S  
Sbjct: 277 ---------------------------------------SSETTDQNWVTGTLAVMGSIT 336

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
           +W+ + ILQ+F  +KYPA+L++ +  C   T  +   +L   RD   WK+  D+  L  V
Sbjct: 337 TWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDSGTLAAV 351

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A     V +E++H+GS++GA FI
Sbjct: 397 YSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSIIGAIFI 351

Query: 455 VLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTS 492
           V GLY V+WGK  D V++ E+   + G+ +  + NTS
Sbjct: 457 VFGLYSVVWGKAKDEVISVEE---KIGMQELPITNTS 351

BLAST of Cp4.1LG00g00920 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.3e-45
Identity = 128/439 (29.16%), Postives = 188/439 (42.82%), Query Frame = 0

Query: 32  NWDLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFY 91
           N  + P V   YRHI+A + + PFAY +DR  R  +T+S+  KI LL +L  TI  NL+Y
Sbjct: 31  NQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYY 90

Query: 92  LGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTF 151
            G+KYTSAT  +A +NV P+F F+MA +FRLEKVN+K     AK+LGT++ +GG+++ T 
Sbjct: 91  TGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTV 150

Query: 152 WKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQ 211
            KGP                                                        
Sbjct: 151 VKGP-------------------------------------------------------- 210

Query: 212 WDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARG 271
                                                                       
Sbjct: 211 ------------------------------------------------------------ 270

Query: 272 GAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGK-DWIKGSAFIV 331
                         LI   W  P+               +QDS N+  K D  KG++ I 
Sbjct: 271 --------------LIPLPWANPHDI-------------HQDSSNTGVKQDLTKGASLIA 326

Query: 332 LSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFAR-DPEKWKLQWDAR 391
           +  + W+ ++ LQ    + YP +L++T   C   + +S  +ALF  R +P  W +  D++
Sbjct: 331 IGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSK 326

Query: 392 LLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLV 451
           LL  VY G+I S +GY+VQ  ++   GPVF + FNPL ++ VAI  + + +E + +G ++
Sbjct: 391 LLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRIL 326

Query: 452 GAFFIVLGLYLVLWGKKAD 469
           GA  IVLGLY VLWGK  D
Sbjct: 451 GAIVIVLGLYSVLWGKSKD 326

BLAST of Cp4.1LG00g00920 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.3e-45
Identity = 121/436 (27.75%), Postives = 193/436 (44.27%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           +D +V   YRH++A +V+ PFA + +R  R  +T+++  ++  L IL   +  NL+Y+G+
Sbjct: 37  MDHWVLATYRHVVATVVMAPFALMFERKIRPKMTLAIFWRLLALGILEPLMDQNLYYIGL 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
           K TSA+  SAF+N  P+ TF++A++FRLE VN +     AKV+GTVI +GG++I T +KG
Sbjct: 97  KNTSASYTSAFTNALPAVTFILALIFRLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P +                                                         
Sbjct: 157 PAIE-------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                                      + + +  S+  GSS+T                 
Sbjct: 217 ---------------------------IVKAAHNSFHGGSSST----------------- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                      +G+ W+ G+  I+ S  
Sbjct: 277 ------------------------------------------PTGQHWVLGTIAIMGSIS 330

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
           +W+A+ ILQ++  + YPA+L++  L C   T  +   +L   RDP  WK+  D+  L  V
Sbjct: 337 TWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMDSGTLAAV 330

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A   A V +E++H+GS++GA FI
Sbjct: 397 YSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGSIIGAVFI 330

Query: 455 VLGLYLVLWGKKADHV 471
           VLGLY V+WGK  D V
Sbjct: 457 VLGLYSVVWGKSKDEV 330

BLAST of Cp4.1LG00g00920 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.1e-44
Identity = 130/443 (29.35%), Postives = 190/443 (42.89%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           ++ +V  VYRH IA  V+ PFA   +R  R  +T  + ++I LL  +   +  NL+Y+G+
Sbjct: 37  MNHYVLAVYRHAIATAVIAPFALFHERKIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGM 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
            YTSAT ASA +NV P+ TFV+A++FRLE VN K  R  AKV+GTVI + G+L+ T +KG
Sbjct: 97  TYTSATFASATANVLPAITFVLAIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P                                                           
Sbjct: 157 P----------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                              I D      F R G     GS    G         + GGA 
Sbjct: 217 -------------------IVD------FIRFGGGGGGGSDGAGG---------SHGGA- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                     +  K WI G+  ++    
Sbjct: 277 ---------------------------------------GAAAMDKHWIPGTLMLLGRTF 336

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
            W+ + ILQ+F  ++YPA+L++T L C+  T +   ++L   RD   WK+ +D+ L    
Sbjct: 337 GWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLSAWKIGFDSNLFAAA 346

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG+I S V Y+VQ  V+   GPVF + FNPL ++  A     V SE +H+GS++G  FI
Sbjct: 397 YSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALGVVVLSESIHLGSVIGTLFI 346

Query: 455 VLGLYLVLWGKKADHVVADEQQD 478
           ++GLY V+WGK  D  + D+ +D
Sbjct: 457 IVGLYTVVWGKGKDKRMTDDDED 346

BLAST of Cp4.1LG00g00920 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.7e-43
Identity = 126/450 (28.00%), Postives = 200/450 (44.44%), Query Frame = 0

Query: 36  DPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGIK 95
           +PFVF  YR   A L L PFA+ ++ +K + L+  + +KIF +++ G T+ LNL+Y+ I+
Sbjct: 34  NPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFILLLKIFFISLCGLTLSLNLYYVAIE 93

Query: 96  YTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGP 155
            T+AT A+A +N  PS TFV+A+LFRLE V +K + G AKV G+++ + G+L+F F KGP
Sbjct: 94  NTTATFAAATTNAIPSITFVLALLFRLETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP 153

Query: 156 YLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDAR 215
                     LIN Y +    N                                      
Sbjct: 154 ---------SLINHYNSSTIPN-------------------------------------- 213

Query: 216 LLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAKV 275
                                                                       
Sbjct: 214 ------------------------------------------------------------ 273

Query: 276 LGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEMS 335
            GTV                                      S K+ +KGS  ++ +   
Sbjct: 274 -GTV-------------------------------------PSTKNSVKGSITMLAANTC 333

Query: 336 WSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIVY 395
           W  W+I+Q+ V ++YPAKL +  L C+F+  QS   A+   R+P  WK+++   LL++ Y
Sbjct: 334 WCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFGLPLLSMAY 338

Query: 396 SGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFIV 455
            GI+++ + Y++Q W I   GPVFT+++ PL LI   I S+F+F E  ++GS+ GA  +V
Sbjct: 394 CGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGSVGGAVLLV 338

Query: 456 LGLYLVLWGK-KADHVVADEQQDNEKGLVD 485
            GLYL LWGK K + +    ++ ++K +++
Sbjct: 454 CGLYLGLWGKTKEEEIQRYGEKQSQKEIIE 338

BLAST of Cp4.1LG00g00920 vs. NCBI nr
Match: XP_023520558.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580 bits (1495), Expect = 5.54e-204
Identity = 328/467 (70.24%), Postives = 328/467 (70.24%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK
Sbjct: 92  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
           GPYLTKAIFKEPLINIYKN                                         
Sbjct: 152 GPYLTKAIFKEPLINIYKN----------------------------------------- 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                                       
Sbjct: 212 ------------------------------------------------------------ 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
                                                 QDSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 --------------------------------------QDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 359

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 359

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 359

BLAST of Cp4.1LG00g00920 vs. NCBI nr
Match: XP_022928955.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 563 bits (1452), Expect = 1.90e-197
Identity = 320/467 (68.52%), Postives = 322/467 (68.95%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKR SLTISMTIKIFLLAILGST YLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRASLTISMTIKIFLLAILGSTTYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK
Sbjct: 92  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
           GPYLTKAIFKEPLINIYKN                                         
Sbjct: 152 GPYLTKAIFKEPLINIYKN----------------------------------------- 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                                       
Sbjct: 212 ------------------------------------------------------------ 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
                                                 QDSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 --------------------------------------QDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 359

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 359

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQ+ EK   DTEMQ+TSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKDS 359

BLAST of Cp4.1LG00g00920 vs. NCBI nr
Match: XP_022970057.1 (WAT1-related protein At1g43650-like [Cucurbita maxima])

HSP 1 Score: 559 bits (1441), Expect = 1.15e-195
Identity = 318/467 (68.09%), Postives = 321/467 (68.74%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNK+TSLTIS TIKIFLLAILGST YLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKQTSLTISTTIKIFLLAILGSTTYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARG AKVLGTVICIGGSLIFTFWK
Sbjct: 92  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGAAKVLGTVICIGGSLIFTFWK 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
           GPYLTKAIFKEPLINIYKN                                         
Sbjct: 152 GPYLTKAIFKEPLINIYKN----------------------------------------- 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                                       
Sbjct: 212 ------------------------------------------------------------ 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
                                                 QDSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 --------------------------------------QDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKL WDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLHWDARLLTI 359

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 359

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQ+ EK  VDTEMQ+TSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTSVNDNEVKDS 359

BLAST of Cp4.1LG00g00920 vs. NCBI nr
Match: KAG6579609.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504 bits (1299), Expect = 1.28e-174
Identity = 293/467 (62.74%), Postives = 296/467 (63.38%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKR SLTISMTIKIFLLAILGST YLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRASLTISMTIKIFLLAILGSTTYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IK                                                          
Sbjct: 92  IK---------------------------------------------------------- 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
                                                                       
Sbjct: 152 ------------------------------------------------------------ 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                          LEKVNIKTARGGA
Sbjct: 212 -----------------------------------------------LEKVNIKTARGGA 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
           KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 333

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 333

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQ+ EK   DTEMQ+TSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKDS 333

BLAST of Cp4.1LG00g00920 vs. NCBI nr
Match: KAG6579613.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488 bits (1255), Expect = 1.21e-167
Identity = 281/489 (57.46%), Postives = 309/489 (63.19%), Query Frame = 0

Query: 20  AVCVVCSNGGGANW--------DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISM 79
           A+ VV    GG+N         DL+PFVF VYRH IAFL+L PFAYVIDRNK  SLTIS 
Sbjct: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRNKPASLTIST 69

Query: 80  TIKIFLLAILGSTIYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTAR 139
            IKIFLLAILGSTI+LNLFY+GI YTS TVASAFSNV PS TFVMAVLFRLEKVNI+TAR
Sbjct: 70  AIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRLEKVNIRTAR 129

Query: 140 GGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFAT 199
           G AKV+GT++CIGGSLIFT WKGPY+TKAIFKEPLI+IYKN DSGN              
Sbjct: 130 GAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGN-------------- 189

Query: 200 AQSGFLALFFARDPEKWKLQWDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYK 259
                                                                       
Sbjct: 190 ------------------------------------------------------------ 249

Query: 260 PGSSATMGQLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN 319
                                                                       
Sbjct: 250 ------------------------------------------------------------ 309

Query: 320 QDSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFL 379
                SSGKDWIKGSAFI++S+++WS WLILQ FVTRKYPA+LT+TVL C+FATAQSGFL
Sbjct: 310 -----SSGKDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFL 359

Query: 380 ALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFV 439
           AL FARDPEKWKLQWDARLLTIVYSGI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFV
Sbjct: 370 ALIFARDPEKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFV 359

Query: 440 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTS 499
           AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVA E Q+ +K  VDTEMQNTS
Sbjct: 430 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 359

BLAST of Cp4.1LG00g00920 vs. ExPASy TrEMBL
Match: A0A6J1ESW6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1)

HSP 1 Score: 563 bits (1452), Expect = 9.19e-198
Identity = 320/467 (68.52%), Postives = 322/467 (68.95%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKR SLTISMTIKIFLLAILGST YLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRASLTISMTIKIFLLAILGSTTYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK
Sbjct: 92  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
           GPYLTKAIFKEPLINIYKN                                         
Sbjct: 152 GPYLTKAIFKEPLINIYKN----------------------------------------- 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                                       
Sbjct: 212 ------------------------------------------------------------ 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
                                                 QDSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 --------------------------------------QDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 359

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 359

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQ+ EK   DTEMQ+TSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKDS 359

BLAST of Cp4.1LG00g00920 vs. ExPASy TrEMBL
Match: A0A6J1HY24 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1)

HSP 1 Score: 559 bits (1441), Expect = 5.57e-196
Identity = 318/467 (68.09%), Postives = 321/467 (68.74%), Query Frame = 0

Query: 34  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLG 93
           DLDPFVFFVYRHIIAFLVLLPFAYVIDRNK+TSLTIS TIKIFLLAILGST YLNLFYLG
Sbjct: 32  DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKQTSLTISTTIKIFLLAILGSTTYLNLFYLG 91

Query: 94  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWK 153
           IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARG AKVLGTVICIGGSLIFTFWK
Sbjct: 92  IKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGAAKVLGTVICIGGSLIFTFWK 151

Query: 154 GPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWD 213
           GPYLTKAIFKEPLINIYKN                                         
Sbjct: 152 GPYLTKAIFKEPLINIYKN----------------------------------------- 211

Query: 214 ARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGA 273
                                                                       
Sbjct: 212 ------------------------------------------------------------ 271

Query: 274 KVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSE 333
                                                 QDSGNSSGKDWIKGSAFIVLSE
Sbjct: 272 --------------------------------------QDSGNSSGKDWIKGSAFIVLSE 331

Query: 334 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTI 393
           MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKL WDARLLTI
Sbjct: 332 MSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLHWDARLLTI 359

Query: 394 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 453
           VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF
Sbjct: 392 VYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFF 359

Query: 454 IVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVNDNEVKDS 500
           IVLGLYLVLWGKKADHVVADEQQ+ EK  VDTEMQ+TSVNDNEVKDS
Sbjct: 452 IVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTSVNDNEVKDS 359

BLAST of Cp4.1LG00g00920 vs. ExPASy TrEMBL
Match: A0A6J1EQL7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 3.35e-167
Identity = 280/489 (57.26%), Postives = 309/489 (63.19%), Query Frame = 0

Query: 20  AVCVVCSNGGGANW--------DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISM 79
           A+ VV    GG+N         DL+PFVF VYRH IAFL+L PFAYVIDRNK  SLTIS 
Sbjct: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRNKPASLTIST 69

Query: 80  TIKIFLLAILGSTIYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTAR 139
            IKIFLLAILGSTI+LNLFY+GI YTS TVASAFSNV PS TFVMAVLFRLEKVNI+TAR
Sbjct: 70  AIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRLEKVNIRTAR 129

Query: 140 GGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFAT 199
           G AKV+GT++CIGGSLIFT WKGPY+TKAIFKEPLI+IYKN DSGN              
Sbjct: 130 GAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGN-------------- 189

Query: 200 AQSGFLALFFARDPEKWKLQWDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYK 259
                                                                       
Sbjct: 190 ------------------------------------------------------------ 249

Query: 260 PGSSATMGQLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN 319
                                                                       
Sbjct: 250 ------------------------------------------------------------ 309

Query: 320 QDSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFL 379
                SSGKDWIKGSAFI++S+++WS WLILQ FVTRKYPA+LT+TVL C+FATAQSGFL
Sbjct: 310 -----SSGKDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFL 359

Query: 380 ALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFV 439
           AL FARDPEKWKLQWDARLLTIVYSGI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFV
Sbjct: 370 ALIFARDPEKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFV 359

Query: 440 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTS 499
           AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVA E Q+ +K  VDTEMQ+TS
Sbjct: 430 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTS 359

BLAST of Cp4.1LG00g00920 vs. ExPASy TrEMBL
Match: A0A6J1I2R9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1)

HSP 1 Score: 485 bits (1248), Expect = 6.74e-167
Identity = 279/487 (57.29%), Postives = 307/487 (63.04%), Query Frame = 0

Query: 20  AVCVVCSNGGGANW--------DLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISM 79
           A+ VV    GG+N         DL+PFVF VYRH IAFL+L PFAYVIDRNK  SLTIS 
Sbjct: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRNKPASLTIST 69

Query: 80  TIKIFLLAILGSTIYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTAR 139
            IKIFLLAILGSTI+LNLFY+GI YTS TVASAFSNV PS TFVMAVLFRLEKVNI+TAR
Sbjct: 70  AIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRLEKVNIRTAR 129

Query: 140 GGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFAT 199
           G AKV GT++CIGGSLIFT WKGPY+TKAIFKEPLI+IY+N DSGN              
Sbjct: 130 GAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGN-------------- 189

Query: 200 AQSGFLALFFARDPEKWKLQWDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYK 259
                                                                       
Sbjct: 190 ------------------------------------------------------------ 249

Query: 260 PGSSATMGQLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN 319
                                                                       
Sbjct: 250 ------------------------------------------------------------ 309

Query: 320 QDSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFL 379
                SSGKDWIKGSAFI++S+++WS WLILQ FVTRKYPA+LT+TVL C+FATAQSGFL
Sbjct: 310 -----SSGKDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFL 357

Query: 380 ALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFV 439
           AL FARDPEKWKLQWDARLLTIVYSGI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFV
Sbjct: 370 ALIFARDPEKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFV 357

Query: 440 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTS 498
           AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVA E Q+ +K  VDTEMQNTS
Sbjct: 430 AIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 357

BLAST of Cp4.1LG00g00920 vs. ExPASy TrEMBL
Match: A0A6J1EQM5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 1.81e-144
Identity = 242/444 (54.50%), Postives = 267/444 (60.14%), Query Frame = 0

Query: 31  ANWDLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLF 90
           +N  L+P VF VYRH IAFLVL PFAY+IDRNK TSLTISMTI IFLLAILGSTI+LNLF
Sbjct: 36  SNKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLF 95

Query: 91  YLGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFT 150
           Y GI YTS  V S F+N+ PS TFVMA LFRLE+VNI+TARGGAKVLGT+ CIGGS +FT
Sbjct: 96  YAGISYTSPIVTSTFNNIIPSLTFVMAFLFRLERVNIRTARGGAKVLGTITCIGGSFVFT 155

Query: 151 FWKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKL 210
           FWKGPY+TK IFKEPLI++YKN                                      
Sbjct: 156 FWKGPYVTKGIFKEPLIDVYKN-------------------------------------- 215

Query: 211 QWDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTAR 270
                                                                       
Sbjct: 216 ------------------------------------------------------------ 275

Query: 271 GGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIV 330
                                                    Q  G+ SG+DWIKGSA IV
Sbjct: 276 -----------------------------------------QGCGHRSGEDWIKGSALIV 335

Query: 331 LSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARL 390
           +SE++WSAWLIL T VTRKYPA+LTITVL C+FATAQSGFLAL FARD EKWKL WDARL
Sbjct: 336 VSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWDARL 340

Query: 391 LTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVG 450
           LTIVY G++IS  GYF+QTW IS NGPVFTS+FNPLMLIFVAIFSAFVFSERLHVGSLVG
Sbjct: 396 LTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGSLVG 340

Query: 451 AFFIVLGLYLVLWGKKADHVVADE 474
           AF I+LGLYLVLWGKKADH VA E
Sbjct: 456 AFLIILGLYLVLWGKKADHEVAPE 340

BLAST of Cp4.1LG00g00920 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-47
Identity = 124/457 (27.13%), Postives = 203/457 (44.42%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           ++ ++   YRH++A +V+ PFA +++R  R  +T  + ++I  L  L   +  NL+Y+G+
Sbjct: 37  MNHWILATYRHVVATIVIAPFALILERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGM 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
           K TSAT +SAF N  P+ TF+MAV+FR+E VN+K  R  AKV+GT I +GG+++ T +KG
Sbjct: 97  KATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P +                                                         
Sbjct: 157 PAIE-------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                                      LF+ +  S   GSS T                 
Sbjct: 217 ---------------------------LFKTAHSSLHGGSSGT----------------- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                  S  ++ ++W+ G+  ++ S  
Sbjct: 277 ---------------------------------------SSETTDQNWVTGTLAVMGSIT 336

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
           +W+ + ILQ+F  +KYPA+L++ +  C   T  +   +L   RD   WK+  D+  L  V
Sbjct: 337 TWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDSGTLAAV 351

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A     V +E++H+GS++GA FI
Sbjct: 397 YSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSIIGAIFI 351

Query: 455 VLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTS 492
           V GLY V+WGK  D V++ E+   + G+ +  + NTS
Sbjct: 457 VFGLYSVVWGKAKDEVISVEE---KIGMQELPITNTS 351

BLAST of Cp4.1LG00g00920 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 185.7 bits (470), Expect = 9.4e-47
Identity = 121/436 (27.75%), Postives = 193/436 (44.27%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           +D +V   YRH++A +V+ PFA + +R  R  +T+++  ++  L IL   +  NL+Y+G+
Sbjct: 37  MDHWVLATYRHVVATVVMAPFALMFERKIRPKMTLAIFWRLLALGILEPLMDQNLYYIGL 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
           K TSA+  SAF+N  P+ TF++A++FRLE VN +     AKV+GTVI +GG++I T +KG
Sbjct: 97  KNTSASYTSAFTNALPAVTFILALIFRLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P +                                                         
Sbjct: 157 PAIE-------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                                      + + +  S+  GSS+T                 
Sbjct: 217 ---------------------------IVKAAHNSFHGGSSST----------------- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                      +G+ W+ G+  I+ S  
Sbjct: 277 ------------------------------------------PTGQHWVLGTIAIMGSIS 330

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
           +W+A+ ILQ++  + YPA+L++  L C   T  +   +L   RDP  WK+  D+  L  V
Sbjct: 337 TWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMDSGTLAAV 330

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A   A V +E++H+GS++GA FI
Sbjct: 397 YSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGSIIGAVFI 330

Query: 455 VLGLYLVLWGKKADHV 471
           VLGLY V+WGK  D V
Sbjct: 457 VLGLYSVVWGKSKDEV 330

BLAST of Cp4.1LG00g00920 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 185.7 bits (470), Expect = 9.4e-47
Identity = 128/439 (29.16%), Postives = 188/439 (42.82%), Query Frame = 0

Query: 32  NWDLDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFY 91
           N  + P V   YRHI+A + + PFAY +DR  R  +T+S+  KI LL +L  TI  NL+Y
Sbjct: 31  NQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYY 90

Query: 92  LGIKYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTF 151
            G+KYTSAT  +A +NV P+F F+MA +FRLEKVN+K     AK+LGT++ +GG+++ T 
Sbjct: 91  TGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTV 150

Query: 152 WKGPYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQ 211
            KGP                                                        
Sbjct: 151 VKGP-------------------------------------------------------- 210

Query: 212 WDARLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARG 271
                                                                       
Sbjct: 211 ------------------------------------------------------------ 270

Query: 272 GAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGK-DWIKGSAFIV 331
                         LI   W  P+               +QDS N+  K D  KG++ I 
Sbjct: 271 --------------LIPLPWANPHDI-------------HQDSSNTGVKQDLTKGASLIA 326

Query: 332 LSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFAR-DPEKWKLQWDAR 391
           +  + W+ ++ LQ    + YP +L++T   C   + +S  +ALF  R +P  W +  D++
Sbjct: 331 IGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSK 326

Query: 392 LLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLV 451
           LL  VY G+I S +GY+VQ  ++   GPVF + FNPL ++ VAI  + + +E + +G ++
Sbjct: 391 LLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRIL 326

Query: 452 GAFFIVLGLYLVLWGKKAD 469
           GA  IVLGLY VLWGK  D
Sbjct: 451 GAIVIVLGLYSVLWGKSKD 326

BLAST of Cp4.1LG00g00920 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 182.6 bits (462), Expect = 8.0e-46
Identity = 130/443 (29.35%), Postives = 190/443 (42.89%), Query Frame = 0

Query: 35  LDPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGI 94
           ++ +V  VYRH IA  V+ PFA   +R  R  +T  + ++I LL  +   +  NL+Y+G+
Sbjct: 37  MNHYVLAVYRHAIATAVIAPFALFHERKIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGM 96

Query: 95  KYTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKG 154
            YTSAT ASA +NV P+ TFV+A++FRLE VN K  R  AKV+GTVI + G+L+ T +KG
Sbjct: 97  TYTSATFASATANVLPAITFVLAIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKG 156

Query: 155 PYLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDA 214
           P                                                           
Sbjct: 157 P----------------------------------------------------------- 216

Query: 215 RLLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAK 274
                              I D      F R G     GS    G         + GGA 
Sbjct: 217 -------------------IVD------FIRFGGGGGGGSDGAGG---------SHGGA- 276

Query: 275 VLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEM 334
                                                     +  K WI G+  ++    
Sbjct: 277 ---------------------------------------GAAAMDKHWIPGTLMLLGRTF 336

Query: 335 SWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIV 394
            W+ + ILQ+F  ++YPA+L++T L C+  T +   ++L   RD   WK+ +D+ L    
Sbjct: 337 GWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLSAWKIGFDSNLFAAA 346

Query: 395 YSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFI 454
           YSG+I S V Y+VQ  V+   GPVF + FNPL ++  A     V SE +H+GS++G  FI
Sbjct: 397 YSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALGVVVLSESIHLGSVIGTLFI 346

Query: 455 VLGLYLVLWGKKADHVVADEQQD 478
           ++GLY V+WGK  D  + D+ +D
Sbjct: 457 IVGLYTVVWGKGKDKRMTDDDED 346

BLAST of Cp4.1LG00g00920 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.3e-44
Identity = 126/450 (28.00%), Postives = 200/450 (44.44%), Query Frame = 0

Query: 36  DPFVFFVYRHIIAFLVLLPFAYVIDRNKRTSLTISMTIKIFLLAILGSTIYLNLFYLGIK 95
           +PFVF  YR   A L L PFA+ ++ +K + L+  + +KIF +++ G T+ LNL+Y+ I+
Sbjct: 34  NPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFILLLKIFFISLCGLTLSLNLYYVAIE 93

Query: 96  YTSATVASAFSNVTPSFTFVMAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGP 155
            T+AT A+A +N  PS TFV+A+LFRLE V +K + G AKV G+++ + G+L+F F KGP
Sbjct: 94  NTTATFAAATTNAIPSITFVLALLFRLETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP 153

Query: 156 YLTKAIFKEPLINIYKNEDSGNTKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDAR 215
                     LIN Y +    N                                      
Sbjct: 154 ---------SLINHYNSSTIPN-------------------------------------- 213

Query: 216 LLTIVYSDTSYQLTSKQTITDVCRALLFRRSGISYKPGSSATMGQLEKVNIKTARGGAKV 275
                                                                       
Sbjct: 214 ------------------------------------------------------------ 273

Query: 276 LGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKDWIKGSAFIVLSEMS 335
            GTV                                      S K+ +KGS  ++ +   
Sbjct: 274 -GTV-------------------------------------PSTKNSVKGSITMLAANTC 333

Query: 336 WSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIVY 395
           W  W+I+Q+ V ++YPAKL +  L C+F+  QS   A+   R+P  WK+++   LL++ Y
Sbjct: 334 WCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFGLPLLSMAY 338

Query: 396 SGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFIV 455
            GI+++ + Y++Q W I   GPVFT+++ PL LI   I S+F+F E  ++GS+ GA  +V
Sbjct: 394 CGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGSVGGAVLLV 338

Query: 456 LGLYLVLWGK-KADHVVADEQQDNEKGLVD 485
            GLYL LWGK K + +    ++ ++K +++
Sbjct: 454 CGLYLGLWGKTKEEEIQRYGEKQSQKEIIE 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q501F82.0e-4627.13WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2... [more]
O806381.3e-4529.16WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Q9LPF11.3e-4527.75WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1... [more]
F4HZQ71.1e-4429.35WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Q6NMB74.7e-4328.00WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2... [more]
Match NameE-valueIdentityDescription
XP_023520558.15.54e-20470.24WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo][more]
XP_022928955.11.90e-19768.52WAT1-related protein At1g43650-like [Cucurbita moschata][more]
XP_022970057.11.15e-19568.09WAT1-related protein At1g43650-like [Cucurbita maxima][more]
KAG6579609.11.28e-17462.74WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6579613.11.21e-16757.46WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1ESW69.19e-19868.52WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1[more]
A0A6J1HY245.57e-19668.09WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1[more]
A0A6J1EQL73.35e-16757.26WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1[more]
A0A6J1I2R96.74e-16757.29WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1[more]
A0A6J1EQM51.81e-14454.50WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G08300.11.5e-4727.13nodulin MtN21 /EamA-like transporter family protein [more]
AT1G44800.19.4e-4727.75nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.19.4e-4729.16nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.18.0e-4629.35nodulin MtN21 /EamA-like transporter family protein [more]
AT1G43650.13.3e-4428.00nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 324..462
e-value: 1.0E-15
score: 58.1
coord: 35..144
e-value: 7.0E-16
score: 58.6
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 259..480
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 179..223
coord: 34..175
NoneNo IPR availablePANTHERPTHR31218:SF265WAT1-RELATED PROTEINcoord: 259..480
NoneNo IPR availablePANTHERPTHR31218:SF265WAT1-RELATED PROTEINcoord: 179..223
coord: 34..175
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 370..466
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 43..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g00920.1Cp4.1LG00g00920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity