CmoCh20G011300 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G011300
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionUbiquitin carboxyl-terminal hydrolase
LocationCmo_Chr20: 10928567 .. 10935286 (-)
RNA-Seq ExpressionCmoCh20G011300
SyntenyCmoCh20G011300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAAGCATCTATGAAGAACTTCCTCCGGCGATTACAACCAGCGGGGTTTTACGTGCTTGTTGCTGGACTTCTCAGCGCCGCCGCTCTGTTTTCGGTCATTAGAGATGGTAAACTCAACAATTTAGGCCTTTTATCCTGGACGGCGGGAGCAAGTTCGTCCCAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTCGGGAACAATTGCTTTTTCAATGTGATTTTACAGGTATTATTACTTTCGGCTCATGGATTTTCTTTTTCTACGAGTTTTATCTATGAATTGTACTCATATTCATCTGAATGTTGAAATTTCGTCAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTTCTTCAAAAGCTTATGGAGGAATTTGAACTTCACGAGGAAATGAGTGATGCCTTACCACTTACGATCTCTTTAGCTGCACTATTAGAAGGTACCATCACTTAATTCGCTATTCATTTGAAGTAGTTTTTCGCAAATTAGAAGGGAATGGTGCTCAATTTGACCTGTTTAGATGCAATTCTGTTTCCGCGAATCGTTTTTCGGAATTGATGTTATGGAAATCTTTGATGCAACGTTTATCTTTGAGAAGCTACTGTAATTCCATGTAGTTTTCTAATCATTAAGTATGCTTCTACATATGTAAATGATAAAGATTTAGAAGTAATGAATGACTTTGGGAACATTTAGTATCGTACTAGTTTTCTTTTCAACAAAATGTAGTAGGGTAGGAGAGTTACAAAGTGAGAATAGTTGAGCTGTGCTGGATGTCACAATTGCACTTTTCTTGTCACAATTGCACTTTTCTTGGCCATGGACTTTGCGATTGTGCGGCAGTCACTCTCAACATCAAGTGAGTCAAACCAATTTGTATTAGAGTTGCCTATGGCCGGGCATGCTCAAGCCTTTGGCCTCGTTTGAGAAGAAGGTTTTAGAAAACGGGGCAGAGATATTTTTTAAAGTGAGTAAGTAAGAATGAGAGACGATTTTAAAAGCAATGTTATATAACTATAAGCAAAAGGCAACACAACGGCTTAAACAACATGTAGCTTTCCTGGTAGGGAGAATTTGATACCTAGTCACATCCTCCTATAGTACATTTTGAGGCGATTCACATTTGACAGGCTTAGACATGATTTCTCCGAACAGTCATACAAAATCAGGGGGTGAAGTTGACACTAGTCACACTCCCCTATAGTATATTTTGAGGTGATTCAGGTCTGGCAGGTCTAAATATAATTTCACCGAGTAGTCGTACAAATAGGAAATACAATAGTAAGACAATACAACATAAAATTAAGTAAAGGGTTCGGCGTGACATGGGTATGCGGCCATAGGCAACACGCCCTGGACGAGCATGACCGTGACACTGGACACACGAATATAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAATCACTTATGCTTGCTTTATAAAGTATTTCTAACAAGAAATAATCATTTCTTTGATAAATGAAAGGACCTGTAAGAAGTATTAATAAGTATTCTTTGTTCTCTTCATTCGGTTATGCTATGAAAATCATTTCTGCAGAGCTAAGTGCAATCTCTTCGGAGAGAGTCGTGTTGAGCCCGAGGAAAATAATGCTTGCAATGGCTTATTACGCTCCGAATTTCAATCTAACAAGCCAGCAGGTGTGGCACAGTTGCAGTTATTTCTTTTAATTGTTTTCAGAATCCAACTATGTGTTTCATGTCGTATTGTGTTACTGTCGGTTTGTAGGATGCAGCAGAAGCATTCCTTCATCTTCTTTCTTCATTAAAAGAAGAGATTTCGGATTATAATCATCCGCCGCAGTGTTCGTTAGTCGATGTGTTGGCTTCTAATGGTAGGATTATTACTTCCAGGAGTAAGAGCCCCAGTGAGCTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCCCAGGTTTATTCTGACTTCATAACATTAAATTCATAGGTCTTCTGGGTCATACTTTCTTGTTATTTCTTTATTCTTGTATAAACTTTCGTGTGATTCCCTTGTCGTGAATTTTTCTGCATGTTACTGGTAATGAAACATTTTATTAAGTTGGAAAACTTTAACTCATGGTGGATTTATCTTACCTTTTACATTCCCAATTGTAATGGCCCAAGCCCACCGCTAGTAGATATTGTCCTCTTCAGGCTTTCCATTAAGGTTTTTAAAACGCGTATGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAATCGATGTGGGATCTCACATTCCACCCTCCCTTCAGGGCTCAACGTCCTCTGGCACTTGTTCTCTTCTCTAATCGATTTGGGACCCCCAATCCACCCCTTTAGGACCCGGTGTTCTTGCTGGCACACCTGCCTCGTGTCCAACCCCCTTCGGGGCTCAGCCTCCTCGTTCTGATACCATTTGTAACTACCCAAGCCTACCGCTAGCAGATATTGTCATTTTTGGGGTTTCACTCAAGGTTTTTAAAACGCGTATGTTAGAGAGAGATTTCCACACCCTTATAAACAATGCTTCGTTCTCCTCCCCCGCCGATGTGGGATCTCACAATCTATCCCCCTTCGGGGCGCAGCGTCCTCGCTGGCACTCGTTCCCTTCTCCGATCGATGTGGGATCTCACATTTTCTATTCTAGTAGTTCATTTCTCTATATTTTTTATTTGGGCATTTGATTTCTTTGTTCATTAACTGGCTTTTTTTTTTTTTTTTGCAGATCTCACTGAGTTTTGAATCCTTTCACAATTTGCCCCTTTCACCAGTGCTTAGGGGTGATTCTACTATTGTATGTCTGTTCACCATTTTACTCTTTGGTATTGGAGACCATTTCTTAAAATATCATGCCCCATTATTCTTGTATAACACAGCTACATAATGTTTTTCCTGTACTCATAGGCCTTTTCAACTGCAATAGTAGGTTTTCGGCTGTACGTTGCTGGATTGCTTAAAGCAGTTCTTTGCTGCTGAAAAGGTCGAGAATTATCGTTGCAGCTCTTGTTGGCATGATGCTGGCTTGAAGTATCTGTCCTCAATGGGAGCATCTAAAGTAATTCTTTTACTTGGAATTTCATTTCTCTTTCTATATGGATTTCTACCTCCCTTCCCCTGTTTTAAGCTATGGTGTTATTTTTTTCAATTGTTTTGACTTATAAACTGCTGCGGACTCCTCATTGTACAATAGCCTCGTTTTTAGTCTACGTGAGTTTAATTGCTTAATAACTGGTGTTATTCTTTTGAAATTCTTAGATGGAGATTGAAGAACTTATGAAGTGTGATGGGCAAGATTCTTGTGGTTGTGACAGACTTTTTGATTTATATAAAGTTCCATGGTCAAATAAGTTTTCATTCACCTTAAAGCAACTCAGTATTGCTCACTGTCCAAAGGTATGTCGGCTCAAATTTTGTTCATTTATTTTTATTTTCAAGTAAGAAACGAATCATTGATATTCGAGATTGTAAGATCTCACATCGGTTGGGGAGGAGAACAAAACATTCTTTATAAGATTGTAGAGACCTCTCCCTAGTAGACACATTTTAAAAACCTTGAGGGGAAACCCAAAAGAGAAACCCAAAGAAGACAATATTTGCTAGCGGTGGGCTTGGATAGTTACAAATGGTATCAGAGCTAGACGCCGGACGATGTGCCAGTGAGGAGGTTGAGCCCCGAAGGGGGTGGACATGAGGCAATGTGTCAGCAAGGACGTTGGGTCCCGAAGTGGGGTGGATTTGGGGGTCCCACATCGATTAGAGAAGAGAACGNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGTGGATGGTGAGATCCCCCATTAGCAAGAGGTCATCATTTTCCAGCCACAAACAATCAAGGTTCTCCTACTAAACAATACGAAAGTGAGTTGTTCTAGTTGACATTCATTAGTCCGCTTGGATAGGAGCACCTAGTTGATCGCCCAACACAACAACGTAGTTGTTTACGACGTCTATGAATTAATCCCTTGAGGGTGATAAGGAATTTCTTGTGGTAAAGAGTTGATGAGGGGAGGGTCTCATTTGGTTAAGTGAGAGTTAGTGGTGAAGCTTAGAATTAGGAGGTTTAGGGATTGGGAGTCAGCACTCCTGGCTAAGTGGTTGTGGCTTCCTTCATCTAGTGTTCAATCTAGTGTTCCATATAAACACATTGAATTAGATTGTCGTTTATTTGTGAGAAAATACAAAAAGGGTCCGTGCCACAGGATATGTGAAGACTCGAGAACAATTGGGGCTTTACGGATCTAGAATAAACTATCTTCGCAAGAAGTTGAGTGATTGACGTATTTGCTCAGCTTGAGGAAGAGTGTCATAATATATGTTATTATGTATAGTTGTCCTTCATTGTAATGGTGCATTCATTTAGTTTAAGATTCTCTGGTACATCTATGTGTATGGCCATAGTCTAAAATACAACCATTTTCCTAATACTGAGCTTGGTCGAACCAGAGCAGATGCTCGAGAACGTTTGGGTTACATTTGACATGAAGGAAAGATTGCTTCTCTATGATTGAGGATGTTTACTTTATTTGGGGTATATGGATTGACACGAATAATAAGGATTTTAGAGAGGTGGAGAAATCGGGCGAGGAGGTTCGGGAGGGATTGGATTCGGTTAATGGGCTCTTTTGTAATTATGGTCTTGGTAAAACTGAAGTTCCTCTTGCTTCTTTCTTGTGACGGCCGAAACCAACCGTTAACAGATATTGTGCTCTTTGGGCTTTCCCTTTCGAGCTTCCCCTCAAGATTTTTAAAATGCATCTCCTAGGGAGAGATTTCCACACCCTTGTAAAATATGTTTTGTTCTCCTCCCCAATCGATGTGGGATCTCACAATCCACCCCCTTTGGGGCCCAGCGTCTTTGCTGGCACACCGCCTCGTGTCCAACCCTTTCAGAGCTCAGCTTTCTCGCTGACACATCGCCCAATGTTTGGATTTGATACTATTTACAATGGCCCAAGCCCACCGCTAACAGATATTGTCCTCTTTGGGTTTTCCCTTTCGGGCTTCCCCTCAAGGTTCTTAAAACGCGTTTCCTAGGGAGAGGTTTTCACACCCTTATAAAGAATGTTTCGTTCTCCTCCTCAACCAATGTGGGATCTCACAATCTTTCTTTTTTTTTTTTTTTCTGTTTTTTTTGTATGCCCTTATTTTCTTTCAACTTTCTGATTGGAAGCTCGATTTTTTTCTTTAAAAAACGTATAGGATGGATTTAGCATGATACGATCACAATTACCCACAGCTAAGTGTTCTCATCAGTATTTATTTTACTTGCAGATTCTATGCATCCATCTAAAACGTGTTTCAACGAACATGTTCGGTGACGTACTCAAACTTCAGGTATCAAATTTTTCATTAGTACTGACTTTTGACCCCTAAATTAACAAATATGTTGTAGAATGGGAAATGTCTTCATCTTCTAAACATTCTTTTCCTTGTATGATTTTTCTGGTGTGAGCTCCATGGTGAAAGGTTGCATAGCCTTTTATTAACGTTTTGCAATCTCCTTCCTTAGGGCCACATTTCTTTCCCGCTGATTTTGGACATGTCGCCGTTCATGTTGACGGGAGTAGAAATAAAAGACATAGAAAAACAACTCGGAAAGCAACATTCATCAAATAAAAAACCGAGCCACGGTCTCGATGTCTTCAGAAGACAGTTCGATACCACAATGCTAAATTACATAAACGGACTAACAGGAGAGGACGACTACCCTACGAATCTCGCCTCAGGTGTGCCCTTTTGCTCAAAACAATTAAAACCTTCTGCCGTAGAAACCAGTTCTTCTGAAACTCGATCGAGCTCGGAAACCTGGGATTCAAACTTGTGCTCCAGTTCCCTGGTACGTCTAAACTTCTAATTGCTTTCACAGCATGGATTCTTGAAGTCTGATTTAGTTCACTTTTGAGCCTCTGTGGTGCTTGTGTATTCTCTTATCATACAGGACACTAGGACTAGCCAAATCGAACCGTCAGAAACTTATTTGTATCGAGTCGTTTCAGTTGTTCAGCATTTCGGTAGAGCCGGGGGTGGCCATTACACCGTTTACCGAAGAGTTCAAGATAGCGACGCACGTTTGCAGGATGTCTCTACAGAATGGTTTCATGTATCAGATTCAGAAGTTCATCTTGTTTCTGAAGAAGAAGTTCTTGCAGCTGAAGCTACCTTGCTGTTCTATGAGAAAATCTAGAATGCTGGGTGCAACGTGTTCGGTTCGGAGTCTTAAAAGACGAAATTTGTGAAATCTAGCTCACAATTGTTGATCAAATGCCAGCAAGGGATCATCTGCAAACCGGAGAGCCCATATAGAATCAAAAGACAATACTTAGATTTGAATTAGTTATGTTACAGGAAAGAGTTTGATTGTCTTTGGCGATCTTATAAGCATAAGATTATAGGTTCTTTTCAGTAGTTTATGTATGCCGCCATTGGGCTGGAGGTTTACTTCGTAGCCTTCCAGACGCAAACACTAACACTTTTGAATTATAAACTTGATTCAACTTAAAAAAAGAGCTAAAAATTGGATTACAA

mRNA sequence

AAAAGAAGCATCTATGAAGAACTTCCTCCGGCGATTACAACCAGCGGGGTTTTACGTGCTTGTTGCTGGACTTCTCAGCGCCGCCGCTCTGTTTTCGGTCATTAGAGATGGTAAACTCAACAATTTAGGCCTTTTATCCTGGACGGCGGGAGCAAGTTCGTCCCAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTCGGGAACAATTGCTTTTTCAATGTGATTTTACAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTTCTTCAAAAGCTTATGGAGGAATTTGAACTTCACGAGGAAATGAGTGATGCCTTACCACTTACGATCTCTTTAGCTGCACTATTAGAAGAGCTAAGTGCAATCTCTTCGGAGAGAGTCGTGTTGAGCCCGAGGAAAATAATGCTTGCAATGGCTTATTACGCTCCGAATTTCAATCTAACAAGCCAGCAGGATGCAGCAGAAGCATTCCTTCATCTTCTTTCTTCATTAAAAGAAGAGATTTCGGATTATAATCATCCGCCGCAGTGTTCGTTAGTCGATGTGTTGGCTTCTAATGGTAGGATTATTACTTCCAGGAGTAAGAGCCCCAGTGAGCTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCCCAGATCTCACTGAGTTTTGAATCCTTTCACAATTTGCCCCTTTCACCAGTGCTTAGGGGTGATTCTACTATTGTTTTCGGCTGTACGTTGCTGGATTGCTTAAAGCAGTTCTTTGCTGCTGAAAAGGTCGAGAATTATCGTTGCAGCTCTTGTTGGCATGATGCTGGCTTGAAGTATCTGTCCTCAATGGGAGCATCTAAAATGGAGATTGAAGAACTTATGAAGTGTGATGGGCAAGATTCTTGTGGTTGTGACAGACTTTTTGATTTATATAAAGTTCCATGGTCAAATAAGTTTTCATTCACCTTAAAGCAACTCAGTATTGCTCACTGTCCAAAGATTCTATGCATCCATCTAAAACGTGTTTCAACGAACATGTTCGGTGACGTACTCAAACTTCAGGGCCACATTTCTTTCCCGCTGATTTTGGACATGTCGCCGTTCATGTTGACGGGAGTAGAAATAAAAGACATAGAAAAACAACTCGGAAAGCAACATTCATCAAATAAAAAACCGAGCCACGGTCTCGATGTCTTCAGAAGACAGTTCGATACCACAATGCTAAATTACATAAACGGACTAACAGGAGAGGACGACTACCCTACGAATCTCGCCTCAGGTGTGCCCTTTTGCTCAAAACAATTAAAACCTTCTGCCGTAGAAACCAGTTCTTCTGAAACTCGATCGAGCTCGGAAACCTGGGATTCAAACTTGTGCTCCAGTTCCCTGGACACTAGGACTAGCCAAATCGAACCGTCAGAAACTTATTTGTATCGAGTCGTTTCAGTTGTTCAGCATTTCGGTAGAGCCGGGGGTGGCCATTACACCGTTTACCGAAGAGTTCAAGATAGCGACGCACGTTTGCAGGATGTCTCTACAGAATGGTTTCATGTATCAGATTCAGAAGTTCATCTTGTTTCTGAAGAAGAAGTTCTTGCAGCTGAAGCTACCTTGCTGTTCTATGAGAAAATCTAGAATGCTGGGTGCAACGTGTTCGGTTCGGAGTCTTAAAAGACGAAATTTGTGAAATCTAGCTCACAATTGTTGATCAAATGCCAGCAAGGGATCATCTGCAAACCGGAGAGCCCATATAGAATCAAAAGACAATACTTAGATTTGAATTAGTTATGTTACAGGAAAGAGTTTGATTGTCTTTGGCGATCTTATAAGCATAAGATTATAGGTTCTTTTCAGTAGTTTATGTATGCCGCCATTGGGCTGGAGGTTTACTTCGTAGCCTTCCAGACGCAAACACTAACACTTTTGAATTATAAACTTGATTCAACTTAAAAAAAGAGCTAAAAATTGGATTACAA

Coding sequence (CDS)

ATGAAGAACTTCCTCCGGCGATTACAACCAGCGGGGTTTTACGTGCTTGTTGCTGGACTTCTCAGCGCCGCCGCTCTGTTTTCGGTCATTAGAGATGGTAAACTCAACAATTTAGGCCTTTTATCCTGGACGGCGGGAGCAAGTTCGTCCCAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTCGGGAACAATTGCTTTTTCAATGTGATTTTACAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTTCTTCAAAAGCTTATGGAGGAATTTGAACTTCACGAGGAAATGAGTGATGCCTTACCACTTACGATCTCTTTAGCTGCACTATTAGAAGAGCTAAGTGCAATCTCTTCGGAGAGAGTCGTGTTGAGCCCGAGGAAAATAATGCTTGCAATGGCTTATTACGCTCCGAATTTCAATCTAACAAGCCAGCAGGATGCAGCAGAAGCATTCCTTCATCTTCTTTCTTCATTAAAAGAAGAGATTTCGGATTATAATCATCCGCCGCAGTGTTCGTTAGTCGATGTGTTGGCTTCTAATGGTAGGATTATTACTTCCAGGAGTAAGAGCCCCAGTGAGCTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCCCAGATCTCACTGAGTTTTGAATCCTTTCACAATTTGCCCCTTTCACCAGTGCTTAGGGGTGATTCTACTATTGTTTTCGGCTGTACGTTGCTGGATTGCTTAAAGCAGTTCTTTGCTGCTGAAAAGGTCGAGAATTATCGTTGCAGCTCTTGTTGGCATGATGCTGGCTTGAAGTATCTGTCCTCAATGGGAGCATCTAAAATGGAGATTGAAGAACTTATGAAGTGTGATGGGCAAGATTCTTGTGGTTGTGACAGACTTTTTGATTTATATAAAGTTCCATGGTCAAATAAGTTTTCATTCACCTTAAAGCAACTCAGTATTGCTCACTGTCCAAAGATTCTATGCATCCATCTAAAACGTGTTTCAACGAACATGTTCGGTGACGTACTCAAACTTCAGGGCCACATTTCTTTCCCGCTGATTTTGGACATGTCGCCGTTCATGTTGACGGGAGTAGAAATAAAAGACATAGAAAAACAACTCGGAAAGCAACATTCATCAAATAAAAAACCGAGCCACGGTCTCGATGTCTTCAGAAGACAGTTCGATACCACAATGCTAAATTACATAAACGGACTAACAGGAGAGGACGACTACCCTACGAATCTCGCCTCAGGTGTGCCCTTTTGCTCAAAACAATTAAAACCTTCTGCCGTAGAAACCAGTTCTTCTGAAACTCGATCGAGCTCGGAAACCTGGGATTCAAACTTGTGCTCCAGTTCCCTGGACACTAGGACTAGCCAAATCGAACCGTCAGAAACTTATTTGTATCGAGTCGTTTCAGTTGTTCAGCATTTCGGTAGAGCCGGGGGTGGCCATTACACCGTTTACCGAAGAGTTCAAGATAGCGACGCACGTTTGCAGGATGTCTCTACAGAATGGTTTCATGTATCAGATTCAGAAGTTCATCTTGTTTCTGAAGAAGAAGTTCTTGCAGCTGAAGCTACCTTGCTGTTCTATGAGAAAATCTAG

Protein sequence

MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLASNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHISFPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATLLFYEKI
Homology
BLAST of CmoCh20G011300 vs. ExPASy Swiss-Prot
Match: Q9FPS0 (Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana OX=3702 GN=UBP27 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 7.3e-106
Identity = 235/542 (43.36%), Postives = 299/542 (55.17%), Query Frame = 0

Query: 18  AGLLSAAALFSVIRDGKLNNLGLLSWTAG--ASSSQNLRLLPGLQNLGNNCFFNVILQAL 77
           AGLL  A      + G   NL  L   +G    S  +  L+PGLQNLGNNCF NVILQAL
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSFLVPGLQNLGNNCFLNVILQAL 92

Query: 78  ASCVFFQPFLQKLMEEF--ELHEEMSDALPLTISLAALLEELSAISSERVVLSPRKIMLA 137
           ASC  F+ FLQ ++E+    L  E  + LPLT +L+ALL+EL  + S R V +PRK+M+ 
Sbjct: 93  ASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMVT 152

Query: 138 MAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCS-LVDVLASNGRIITSRSK- 197
           +  YA NFNLTSQQDAAEA LHL+SSL+EEI     P Q S L D+L S    + + S+ 
Sbjct: 153 LTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSEG 212

Query: 198 --SPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL-RGDSTIVF 257
                EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LPLSP+L  G   I+ 
Sbjct: 213 LHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIMS 272

Query: 258 GCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDGQDSCGCDR 317
           GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA++ EIE+L  C G+D C C  
Sbjct: 273 GCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAETEIEKLRSCGGEDQCDCKT 332

Query: 318 LFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHISFPLILDMS 377
              L ++PWSN +S  LKQL IA  PK+LCI ++R S NMF +  KL GHI+FPL+L++S
Sbjct: 333 SLHLQRMPWSNSYSHILKQLIIARFPKLLCIQVQRASFNMF-EEFKLSGHIAFPLVLNLS 392

Query: 378 PFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGEDDYPTNLAS 437
            F  + + +                                               N+  
Sbjct: 393 LFTPSSIGV-----------------------------------------------NIEE 452

Query: 438 GVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLYRVVSVVQH 497
            +   S+  KP A +                                   +YR+V+VV+H
Sbjct: 453 RIEMSSEYQKPEASKNHG--------------------------------MYRLVTVVEH 494

Query: 498 FGRAGGGHYTVYRRV----QDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATLLFYE 547
           FGR G GHYTVYR V    Q+ +    D    WF +SDSEV  VSE +VL AEA+LLFYE
Sbjct: 513 FGRTGSGHYTVYRSVRVFSQEEEEEDCDEDLSWFSISDSEVCRVSESDVLGAEASLLFYE 494

BLAST of CmoCh20G011300 vs. ExPASy Swiss-Prot
Match: A4QNN3 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis OX=8364 GN=usp30 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.0e-30
Identity = 132/499 (26.45%), Postives = 216/499 (43.29%), Query Frame = 0

Query: 55  LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEE 114
           L+PGL NLGN CF N +LQ LASC  F  +L     ++      ++   L+++L  LL+ 
Sbjct: 83  LVPGLLNLGNTCFMNSLLQGLASCPSFIRWLADFTSKYRQENNTTEHQHLSVTLLHLLKA 142

Query: 115 L-SAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQC 174
           L +   +E  VL    ++  +  +    +   +QDA E F  L SSL++E     H    
Sbjct: 143 LCNQEGTEDEVLDASPLLEVLRAHRWQISSFEEQDAHELFHVLTSSLEDERDRRPHVTHL 202

Query: 175 SLVDVL---ASNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFES 234
             +D L       R I  R++ P      Q     PF G L S + C+ C  Q  + +++
Sbjct: 203 FDLDSLEFPLEPQRQIHCRTQVPIYPIPSQWKSQHPFHGRLTSNMVCKHCQHQSPMRYDT 262

Query: 235 FHNLPLS-PVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASK 294
           F +L LS PV      I    TL  CL+ F + E V++  C +C              +K
Sbjct: 263 FDSLSLSIPVATWGHPI----TLDQCLQHFISTESVKDVVCENC--------------TK 322

Query: 295 MEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMF 354
           +   ++      +                N+ +  +KQL +   P+ LCIHL+R+S +  
Sbjct: 323 IHAAQIPNSQSVE----------------NRKTTFVKQLKLGKLPQCLCIHLQRLSWSNQ 382

Query: 355 GDVLKLQGHISFP--LILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDT 414
           G  LK   H+ F   L +D   + ++G              S++K+P++ L    +  +T
Sbjct: 383 GSPLKRNEHVQFSEFLAMDRFKYRISGC-------------STSKQPANHLSAAEQ--ET 442

Query: 415 TMLNYINGLTGEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDT 474
           T     +G  G    PT     +PF +     S +    +    ++  W S+        
Sbjct: 443 T-----DGKEGGAQNPT-----MPFLNGACSTSYISPPFTSPLPTNPEWTSS-------- 502

Query: 475 RTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHL 534
                    +YL+R+++VV H G    GH+  YRR   +    +  S +W  +SD  V  
Sbjct: 503 ---------SYLFRLMAVVVHHGDMHSGHFVTYRR-SPAAKNQKLTSQQWLWISDDTVRR 504

Query: 535 VSEEEVLAAEATLLFYEKI 547
            + +EVL++ A LLFYE+I
Sbjct: 563 TNFQEVLSSSAYLLFYERI 504

BLAST of CmoCh20G011300 vs. ExPASy Swiss-Prot
Match: Q70CQ3 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens OX=9606 GN=USP30 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.9e-30
Identity = 138/508 (27.17%), Postives = 215/508 (42.32%), Query Frame = 0

Query: 55  LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFEL-HEEMSDALPLTISLAALLE 114
           L+PGL NLGN CF N +LQ L++C  F  +L++   ++    +E      L+++L  LL+
Sbjct: 66  LVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLK 125

Query: 115 ELSAIS-SERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQ 174
            LS    ++  VL    ++  +  Y    +   +QDA E F  + SSL++E      P  
Sbjct: 126 ALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERD--RQPRV 185

Query: 175 CSLVDV--LASNGRI----ITSRSK-SPSEL-ERWQGHFLVPFDGILGSILTCQSCSSQI 234
             L DV  L     I    IT R++ SP      W+     PF G L S + C+ C  Q 
Sbjct: 186 THLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQH--PFHGRLTSNMVCKHCEHQS 245

Query: 235 SLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSS 294
            + F++F +L LS      +T     TL  CL  F ++E V +  C +C     ++   +
Sbjct: 246 PVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCDNC---TKIEAKGT 305

Query: 295 MGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRV 354
           +   K+E                           ++ +  +KQL +   P+ LCIHL+R+
Sbjct: 306 LNGEKVE---------------------------HQRTTFVKQLKLGKLPQCLCIHLQRL 365

Query: 355 STNMFGDVLKLQGHISFP--LILDMSPFMLTGVEIKDIEKQLGKQHSSNKK----PSHGL 414
           S +  G  LK   H+ F   L++D+  + L G +      +L K      +    P    
Sbjct: 366 SWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDGPGAPT 425

Query: 415 DVFRRQFDTTMLNYINGLTGEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDS 474
            V  +        ++NG       PT L++ +PF      P  V    S           
Sbjct: 426 PVLNQPGAPKTQIFMNGACSPSLLPT-LSAPMPF------PLPVVPDYS----------- 485

Query: 475 NLCSSSLDTRTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWF 534
                           S TYL+R+++VV H G    GH+  YRR   S       S +W 
Sbjct: 486 ----------------SSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWL 502

Query: 535 HVSDSEVHLVSEEEVLAAEATLLFYEKI 547
            VSD  V   S +EVL++ A LLFYE++
Sbjct: 546 WVSDDTVRKASLQEVLSSSAYLLFYERV 502

BLAST of CmoCh20G011300 vs. ExPASy Swiss-Prot
Match: D3ZPG5 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus OX=10116 GN=Usp30 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.3e-27
Identity = 131/514 (25.49%), Postives = 207/514 (40.27%), Query Frame = 0

Query: 55  LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALP-LTISLAALLE 114
           L+PGL NLGN CF N +LQ L++C  F  +L++   ++   ++       L+++L +LL+
Sbjct: 66  LVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLSLLK 125

Query: 115 ELSAIS-SERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQ 174
            LS    +E  VL    ++  +  Y    +   +QDA E F  + SSL++E      P  
Sbjct: 126 ALSCQEVTEEEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERD--RQPRV 185

Query: 175 CSLVDV--------LASNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQI 234
             L DV        +A       +R         W+     PF G L S + C+ C  Q 
Sbjct: 186 THLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNPWKSQH--PFHGRLSSNMVCKHCEHQS 245

Query: 235 SLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSS 294
            + F++F +L LS      +T     TL  CL  F ++E V +  C +C     ++   +
Sbjct: 246 PVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCDNC---TKIEAKGT 305

Query: 295 MGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRV 354
               K+E                           ++ +  +KQL +   P+ LCIHL+R+
Sbjct: 306 QNGEKVE---------------------------HQRTTFVKQLKLGKLPQCLCIHLQRL 365

Query: 355 STNMFGDVLKLQGHISFP--LILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFR 414
           S +  G  LK   H+ F   L++D   + L G            QH      S G     
Sbjct: 366 SWSSQGTPLKRHEHVQFNEFLMMDFYKYRLLG--------HKPSQHGPKATESPG----- 425

Query: 415 RQFDTTMLNYINGLTGEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCS 474
                                   A GV       KP   + ++ +T    + + +  CS
Sbjct: 426 -----------------------SALGVQDTQAAPKPGLSQPAAPKT----QFFMNGACS 485

Query: 475 SSL----------DTRTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQD 534
            SL              +    S  YL+R+++VV H G    GH+  YRR   S      
Sbjct: 486 PSLLPALPSPMAFPLPVAPDYSSSMYLFRLMAVVVHHGDMHSGHFVTYRRSPPSAKNPLS 502

Query: 535 VSTEWFHVSDSEVHLVSEEEVLAAEATLLFYEKI 547
            S +W  +SD  V   S +EVL++ A LLFYE++
Sbjct: 546 TSNQWLWISDDTVRKASLQEVLSSSAYLLFYERV 502

BLAST of CmoCh20G011300 vs. ExPASy Swiss-Prot
Match: Q3UN04 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus OX=10090 GN=Usp30 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 5.2e-27
Identity = 132/514 (25.68%), Postives = 207/514 (40.27%), Query Frame = 0

Query: 55  LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALP-LTISLAALLE 114
           L+PGL NLGN CF N +LQ L++C  F  +L++   ++   ++       L+++L  LL+
Sbjct: 66  LVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLNLLK 125

Query: 115 ELSAIS-SERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQ 174
            LS    +E  VL    ++  +  Y    +   +QDA E F  + SSL++E      P  
Sbjct: 126 ALSCQEVTEDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERD--RQPRV 185

Query: 175 CSLVDV--------LASNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQI 234
             L DV        +A       +R         W+     PF G L S + C+ C  Q 
Sbjct: 186 THLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNHWKSQH--PFHGRLTSNMVCKHCEHQS 245

Query: 235 SLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSS 294
            + F++F +L LS      +T     TL  CL  F ++E V +  C +C     ++   +
Sbjct: 246 PVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCDNC---TKIEARGT 305

Query: 295 MGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRV 354
           +   K+E                           ++ S  +KQL +   P+ LCIHL+R+
Sbjct: 306 LTGEKVE---------------------------HQRSTFVKQLKLGKLPQCLCIHLQRL 365

Query: 355 STNMFGDVLKLQGHISFP--LILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFR 414
           S +  G  LK   H+ F   L++D   + L G                +K   HG     
Sbjct: 366 SWSSHGTPLKRHEHVQFNEFLMMDFYKYRLLG----------------HKPSQHGPKATE 425

Query: 415 RQFDTTMLNYINGLTGEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCS 474
                                   A  V       KP   +  + +T    + + +  CS
Sbjct: 426 N--------------------PGSAPEVQDAQAAPKPGLSQPGAPKT----QIFLNGACS 485

Query: 475 SS-LDTRTSQIE---------PSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQD 534
            S L    S +           S TYL+R+++VV H G    GH+  YRR   S      
Sbjct: 486 PSLLPALPSPVAFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSAKNPLS 502

Query: 535 VSTEWFHVSDSEVHLVSEEEVLAAEATLLFYEKI 547
            S +W  +SD  V   S +EVL++ A LLFYE++
Sbjct: 546 TSNQWLWISDDTVRKASLQEVLSSSAYLLFYERV 502

BLAST of CmoCh20G011300 vs. ExPASy TrEMBL
Match: A0A6J1EIY6 (ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita moschata OX=3662 GN=LOC111434991 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ
Sbjct: 9   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 68

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS
Sbjct: 69  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 128

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA
Sbjct: 129 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 188

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 189 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 248

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG
Sbjct: 249 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 308

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS
Sbjct: 309 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 368

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED
Sbjct: 369 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 428

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY
Sbjct: 429 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 488

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL
Sbjct: 489 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 548

Query: 541 LFYEKI 547
           LFYEKI
Sbjct: 549 LFYEKI 554

BLAST of CmoCh20G011300 vs. ExPASy TrEMBL
Match: A0A6J1I9D4 (ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita maxima OX=3661 GN=LOC111470393 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 1.9e-303
Identity = 526/546 (96.34%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQPAGFYV VAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRL+PGLQ
Sbjct: 9   MKNFLRRLQPAGFYVFVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLVPGLQ 68

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEE+SDALPLTISLAALLEELSAISS
Sbjct: 69  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEISDALPLTISLAALLEELSAISS 128

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRK+MLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPP CSLVD LA
Sbjct: 129 ERVVLSPRKVMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPHCSLVDALA 188

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 189 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 248

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWH AGLKYLSSMGASKMEIEELMKCDG
Sbjct: 249 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHHAGLKYLSSMGASKMEIEELMKCDG 308

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSCGCD LFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD LKLQGHIS
Sbjct: 309 QDSCGCDNLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDALKLQGHIS 368

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLD FRRQFDTTMLNYINGLTGED
Sbjct: 369 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDFFRRQFDTTMLNYINGLTGED 428

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASG PFCSKQLK SA ETSSS+TR+SSET D+NLCSSSLDTRTSQIEPSETY+Y
Sbjct: 429 DYPTNLASGEPFCSKQLKASAEETSSSQTRASSETCDTNLCSSSLDTRTSQIEPSETYMY 488

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSD RLQDVSTEWFHVSDSEVHLVSEEEV+AAEATL
Sbjct: 489 RVVSVVQHFGRAGGGHYTVYRRVQDSDTRLQDVSTEWFHVSDSEVHLVSEEEVIAAEATL 548

Query: 541 LFYEKI 547
           LFYEKI
Sbjct: 549 LFYEKI 554

BLAST of CmoCh20G011300 vs. ExPASy TrEMBL
Match: A0A0A0LJP9 (USP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G298270 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 7.0e-261
Identity = 461/554 (83.21%), Postives = 490/554 (88.45%), Query Frame = 0

Query: 2   KNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQN 61
           +NF+ R QPA F V  AGL+SAAAL S IR GKLN LGLLSWT G SSS+NLRLLPGLQN
Sbjct: 6   ENFITRFQPAEFCVYAAGLVSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLLPGLQN 65

Query: 62  LGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEM-SDALPLTISLAALLEELSAISS 121
           LGNNCFFNVILQALASCVFFQPFLQK +EEFELHE M S+ALPLT+SLAALLEEL  IS 
Sbjct: 66  LGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEELGEISL 125

Query: 122 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 181
           ERVVLSPRK+MLAMAYY PNF+LTSQQDAAEAFLHLLSSLKEEISD+N+P QCSL DV+ 
Sbjct: 126 ERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSLTDVMG 185

Query: 182 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 241
           SNGRIITS ++SP+ELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP+SP L
Sbjct: 186 SNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPAL 245

Query: 242 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 301
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCS CWH+AGLKYLSSMGAS+MEIEELMKCD 
Sbjct: 246 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDR 305

Query: 302 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 361
           QDSCGC RL  LYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD  KLQGHIS
Sbjct: 306 QDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHIS 365

Query: 362 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 421
           FPLILDMSPFMLTGVEIKD+E QLG QHSSNKKPSHGLD FR QFD  ML YINGLTGED
Sbjct: 366 FPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTGED 425

Query: 422 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNL--CSSSLDTRTSQIEPSETY 481
           ++P NL SG P C  + K S  ETSSS+TR SSETWD+N   CSSS +TRT QIEPSET+
Sbjct: 426 EFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIEPSETF 485

Query: 482 LYRVVSVVQHFGRAGGGHYTVYRRV------QDSDARLQDVSTEWFHVSDSEVHLVSEEE 541
           LYRVVSVVQHFG+ GGGHYTVYRRV      QD D + QDVSTEWFHVSDSEVHLVSEEE
Sbjct: 486 LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEE 545

Query: 542 VLAAEATLLFYEKI 547
           VLAAEAT+LFYEKI
Sbjct: 546 VLAAEATMLFYEKI 559

BLAST of CmoCh20G011300 vs. ExPASy TrEMBL
Match: A0A5A7TSR6 (Ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002010 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.8e-254
Identity = 456/554 (82.31%), Postives = 486/554 (87.73%), Query Frame = 0

Query: 2   KNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQN 61
           +N + R QPA F V VAGLLSAAAL S IRDGKLN LGLLSW    SSS+NLRLLPGLQN
Sbjct: 6   ENLITRFQPAEFCVYVAGLLSAAALVSAIRDGKLNILGLLSWAKEPSSSENLRLLPGLQN 65

Query: 62  LGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEM-SDALPLTISLAALLEELSAISS 121
           LGNNCFFNVILQALASCVFFQPFLQK +EEFELHE M  +ALPLT+SLAALLEEL A+S 
Sbjct: 66  LGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTVSLAALLEELGAVSL 125

Query: 122 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 181
           ERVVLSPRK+MLAMAYY PNF+LTSQQDAAEAFLHLLSSLKEEISD+N+P QCSL DV+ 
Sbjct: 126 ERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSLTDVMG 185

Query: 182 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 241
           SNGRIITS ++SP+ELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP+SPVL
Sbjct: 186 SNGRIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPVL 245

Query: 242 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 301
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCS CWH+AGLKYLSSMGAS+MEIEELMKCD 
Sbjct: 246 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDR 305

Query: 302 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 361
           QDSCGC RL  LYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD  KLQGHIS
Sbjct: 306 QDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDTFKLQGHIS 365

Query: 362 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 421
           FPLILDMSPFMLTGVEIKD+  QLG QHSSNKK SHGLD FR QFD  ML YINGLTGED
Sbjct: 366 FPLILDMSPFMLTGVEIKDL-GQLGGQHSSNKKSSHGLDFFRTQFDARMLKYINGLTGED 425

Query: 422 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNL--CSSSLDTRTSQIEPSETY 481
           ++P  L SG   C  ++K S  ETSSS+TR SSETWD+N   CSSS +TRT QIEPSET 
Sbjct: 426 EFPI-LTSGESICYDRVKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETC 485

Query: 482 LYRVVSVVQHFGRAGGGHYTVYRRV------QDSDARLQDVSTEWFHVSDSEVHLVSEEE 541
           LYRVVSVVQHFG+ GGGHYTVYRRV      QD D + Q+VSTEWFHVSDSEVHLVSEEE
Sbjct: 486 LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQEVSTEWFHVSDSEVHLVSEEE 545

Query: 542 VLAAEATLLFYEKI 547
           VLAAEAT+LFYEKI
Sbjct: 546 VLAAEATMLFYEKI 557

BLAST of CmoCh20G011300 vs. ExPASy TrEMBL
Match: A0A1S3C667 (ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo OX=3656 GN=LOC103497488 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.8e-254
Identity = 456/554 (82.31%), Postives = 486/554 (87.73%), Query Frame = 0

Query: 2   KNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQN 61
           +N + R QPA F V VAGLLSAAAL S IRDGKLN LGLLSW    SSS+NLRLLPGLQN
Sbjct: 6   ENLITRFQPAEFCVYVAGLLSAAALVSAIRDGKLNILGLLSWAKEPSSSENLRLLPGLQN 65

Query: 62  LGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEM-SDALPLTISLAALLEELSAISS 121
           LGNNCFFNVILQALASCVFFQPFLQK +EEFELHE M  +ALPLT+SLAALLEEL A+S 
Sbjct: 66  LGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTVSLAALLEELGAVSL 125

Query: 122 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 181
           ERVVLSPRK+MLAMAYY PNF+LTSQQDAAEAFLHLLSSLKEEISD+N+P QCSL DV+ 
Sbjct: 126 ERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSLTDVMG 185

Query: 182 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 241
           SNGRIITS ++SP+ELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP+SPVL
Sbjct: 186 SNGRIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPVL 245

Query: 242 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 301
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCS CWH+AGLKYLSSMGAS+MEIEELMKCD 
Sbjct: 246 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDR 305

Query: 302 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 361
           QDSCGC RL  LYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD  KLQGHIS
Sbjct: 306 QDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDTFKLQGHIS 365

Query: 362 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 421
           FPLILDMSPFMLTGVEIKD+  QLG QHSSNKK SHGLD FR QFD  ML YINGLTGED
Sbjct: 366 FPLILDMSPFMLTGVEIKDL-GQLGGQHSSNKKSSHGLDFFRTQFDARMLKYINGLTGED 425

Query: 422 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNL--CSSSLDTRTSQIEPSETY 481
           ++P  L SG   C  ++K S  ETSSS+TR SSETWD+N   CSSS +TRT QIEPSET 
Sbjct: 426 EFPI-LTSGESICYDRVKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETC 485

Query: 482 LYRVVSVVQHFGRAGGGHYTVYRRV------QDSDARLQDVSTEWFHVSDSEVHLVSEEE 541
           LYRVVSVVQHFG+ GGGHYTVYRRV      QD D + Q+VSTEWFHVSDSEVHLVSEEE
Sbjct: 486 LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQEVSTEWFHVSDSEVHLVSEEE 545

Query: 542 VLAAEATLLFYEKI 547
           VLAAEAT+LFYEKI
Sbjct: 546 VLAAEATMLFYEKI 557

BLAST of CmoCh20G011300 vs. NCBI nr
Match: XP_022928102.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita moschata])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ
Sbjct: 9   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 68

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS
Sbjct: 69  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 128

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA
Sbjct: 129 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 188

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 189 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 248

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG
Sbjct: 249 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 308

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS
Sbjct: 309 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 368

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED
Sbjct: 369 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 428

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY
Sbjct: 429 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 488

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL
Sbjct: 489 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 548

Query: 541 LFYEKI 547
           LFYEKI
Sbjct: 549 LFYEKI 554

BLAST of CmoCh20G011300 vs. NCBI nr
Match: KAG6571374.1 (Ubiquitin carboxyl-terminal hydrolase 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.1e-304
Identity = 530/546 (97.07%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQPAGFYV VAGL+SAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ
Sbjct: 1   MKNFLRRLQPAGFYVFVAGLVSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLA+LLEELSAISS
Sbjct: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLASLLEELSAISS 120

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRK+MLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA
Sbjct: 121 ERVVLSPRKVMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG
Sbjct: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD LKLQGHIS
Sbjct: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDALKLQGHIS 360

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDM PFMLTGVEIKDIEKQLGKQHSSNKKPSHGLD FRRQFDTTMLNYINGLTGED
Sbjct: 361 FPLILDMLPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDFFRRQFDTTMLNYINGLTGED 420

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASG PFCSKQLKPSA ETSSSETR+SSETWD      SLDTRTSQIEPSETYLY
Sbjct: 421 DYPTNLASGEPFCSKQLKPSAEETSSSETRASSETWD------SLDTRTSQIEPSETYLY 480

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL
Sbjct: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540

Query: 541 LFYEKI 547
           LFYEKI
Sbjct: 541 LFYEKI 540

BLAST of CmoCh20G011300 vs. NCBI nr
Match: XP_022971724.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita maxima])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.0e-303
Identity = 526/546 (96.34%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQPAGFYV VAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRL+PGLQ
Sbjct: 9   MKNFLRRLQPAGFYVFVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLVPGLQ 68

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEE+SDALPLTISLAALLEELSAISS
Sbjct: 69  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEISDALPLTISLAALLEELSAISS 128

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRK+MLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPP CSLVD LA
Sbjct: 129 ERVVLSPRKVMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPHCSLVDALA 188

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 189 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 248

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWH AGLKYLSSMGASKMEIEELMKCDG
Sbjct: 249 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHHAGLKYLSSMGASKMEIEELMKCDG 308

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSCGCD LFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD LKLQGHIS
Sbjct: 309 QDSCGCDNLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDALKLQGHIS 368

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLD FRRQFDTTMLNYINGLTGED
Sbjct: 369 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDFFRRQFDTTMLNYINGLTGED 428

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASG PFCSKQLK SA ETSSS+TR+SSET D+NLCSSSLDTRTSQIEPSETY+Y
Sbjct: 429 DYPTNLASGEPFCSKQLKASAEETSSSQTRASSETCDTNLCSSSLDTRTSQIEPSETYMY 488

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSD RLQDVSTEWFHVSDSEVHLVSEEEV+AAEATL
Sbjct: 489 RVVSVVQHFGRAGGGHYTVYRRVQDSDTRLQDVSTEWFHVSDSEVHLVSEEEVIAAEATL 548

Query: 541 LFYEKI 547
           LFYEKI
Sbjct: 549 LFYEKI 554

BLAST of CmoCh20G011300 vs. NCBI nr
Match: XP_023513383.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 523/546 (95.79%), Postives = 530/546 (97.07%), Query Frame = 0

Query: 1   MKNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQ 60
           MKNFLRRLQP GFYV VAGLLSAAALFSVIRDGKLNN+GLLSWTAGASSSQNLRL+PGLQ
Sbjct: 9   MKNFLRRLQPVGFYVFVAGLLSAAALFSVIRDGKLNNIGLLSWTAGASSSQNLRLVPGLQ 68

Query: 61  NLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEELSAISS 120
           NLGNNCFFNVILQALASCVFFQPFL KLMEEFELHEEMSDALPLTISLAALLEELSAISS
Sbjct: 69  NLGNNCFFNVILQALASCVFFQPFLHKLMEEFELHEEMSDALPLTISLAALLEELSAISS 128

Query: 121 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 180
           ERVVLSPRK+MLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA
Sbjct: 129 ERVVLSPRKVMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 188

Query: 181 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 240
           SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL
Sbjct: 189 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 248

Query: 241 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 300
           RGDSTIVFGCTL+DCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG
Sbjct: 249 RGDSTIVFGCTLMDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 308

Query: 301 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 360
           QDSC CDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD LKLQGHIS
Sbjct: 309 QDSCVCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDALKLQGHIS 368

Query: 361 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 420
           FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSH LD FRRQFDTTMLNYINGLTGED
Sbjct: 369 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHRLDFFRRQFDTTMLNYINGLTGED 428

Query: 421 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLY 480
           DYPTNLASG PFCS QLKPSA ETSSSETR+SSETWD      SLDTRTSQIEPSET+LY
Sbjct: 429 DYPTNLASGEPFCSNQLKPSAEETSSSETRASSETWD------SLDTRTSQIEPSETFLY 488

Query: 481 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 540
           RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL
Sbjct: 489 RVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATL 548

Query: 541 LFYEKI 547
           LFYE+I
Sbjct: 549 LFYERI 548

BLAST of CmoCh20G011300 vs. NCBI nr
Match: XP_004148458.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus])

HSP 1 Score: 909.4 bits (2349), Expect = 1.4e-260
Identity = 461/554 (83.21%), Postives = 490/554 (88.45%), Query Frame = 0

Query: 2   KNFLRRLQPAGFYVLVAGLLSAAALFSVIRDGKLNNLGLLSWTAGASSSQNLRLLPGLQN 61
           +NF+ R QPA F V  AGL+SAAAL S IR GKLN LGLLSWT G SSS+NLRLLPGLQN
Sbjct: 6   ENFITRFQPAEFCVYAAGLVSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLLPGLQN 65

Query: 62  LGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEM-SDALPLTISLAALLEELSAISS 121
           LGNNCFFNVILQALASCVFFQPFLQK +EEFELHE M S+ALPLT+SLAALLEEL  IS 
Sbjct: 66  LGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEELGEISL 125

Query: 122 ERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCSLVDVLA 181
           ERVVLSPRK+MLAMAYY PNF+LTSQQDAAEAFLHLLSSLKEEISD+N+P QCSL DV+ 
Sbjct: 126 ERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSLTDVMG 185

Query: 182 SNGRIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL 241
           SNGRIITS ++SP+ELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP+SP L
Sbjct: 186 SNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPAL 245

Query: 242 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDG 301
           RGDSTIVFGCTLLDCLKQFFAAEKVENYRCS CWH+AGLKYLSSMGAS+MEIEELMKCD 
Sbjct: 246 RGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDR 305

Query: 302 QDSCGCDRLFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHIS 361
           QDSCGC RL  LYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD  KLQGHIS
Sbjct: 306 QDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHIS 365

Query: 362 FPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGED 421
           FPLILDMSPFMLTGVEIKD+E QLG QHSSNKKPSHGLD FR QFD  ML YINGLTGED
Sbjct: 366 FPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTGED 425

Query: 422 DYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNL--CSSSLDTRTSQIEPSETY 481
           ++P NL SG P C  + K S  ETSSS+TR SSETWD+N   CSSS +TRT QIEPSET+
Sbjct: 426 EFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIEPSETF 485

Query: 482 LYRVVSVVQHFGRAGGGHYTVYRRV------QDSDARLQDVSTEWFHVSDSEVHLVSEEE 541
           LYRVVSVVQHFG+ GGGHYTVYRRV      QD D + QDVSTEWFHVSDSEVHLVSEEE
Sbjct: 486 LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEE 545

Query: 542 VLAAEATLLFYEKI 547
           VLAAEAT+LFYEKI
Sbjct: 546 VLAAEATMLFYEKI 559

BLAST of CmoCh20G011300 vs. TAIR 10
Match: AT4G39370.1 (ubiquitin-specific protease 27 )

HSP 1 Score: 386.0 bits (990), Expect = 5.2e-107
Identity = 235/542 (43.36%), Postives = 299/542 (55.17%), Query Frame = 0

Query: 18  AGLLSAAALFSVIRDGKLNNLGLLSWTAG--ASSSQNLRLLPGLQNLGNNCFFNVILQAL 77
           AGLL  A      + G   NL  L   +G    S  +  L+PGLQNLGNNCF NVILQAL
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSFLVPGLQNLGNNCFLNVILQAL 92

Query: 78  ASCVFFQPFLQKLMEEF--ELHEEMSDALPLTISLAALLEELSAISSERVVLSPRKIMLA 137
           ASC  F+ FLQ ++E+    L  E  + LPLT +L+ALL+EL  + S R V +PRK+M+ 
Sbjct: 93  ASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMVT 152

Query: 138 MAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCS-LVDVLASNGRIITSRSK- 197
           +  YA NFNLTSQQDAAEA LHL+SSL+EEI     P Q S L D+L S    + + S+ 
Sbjct: 153 LTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSEG 212

Query: 198 --SPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL-RGDSTIVF 257
                EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LPLSP+L  G   I+ 
Sbjct: 213 LHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIMS 272

Query: 258 GCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDGQDSCGCDR 317
           GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA++ EIE+L  C G+D C C  
Sbjct: 273 GCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAETEIEKLRSCGGEDQCDCKT 332

Query: 318 LFDLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHISFPLILDMS 377
              L ++PWSN +S  LKQL IA  PK+LCI ++R S NMF +  KL GHI+FPL+L++S
Sbjct: 333 SLHLQRMPWSNSYSHILKQLIIARFPKLLCIQVQRASFNMF-EEFKLSGHIAFPLVLNLS 392

Query: 378 PFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLTGEDDYPTNLAS 437
            F  + + +                                               N+  
Sbjct: 393 LFTPSSIGV-----------------------------------------------NIEE 452

Query: 438 GVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLYRVVSVVQH 497
            +   S+  KP A +                                   +YR+V+VV+H
Sbjct: 453 RIEMSSEYQKPEASKNHG--------------------------------MYRLVTVVEH 494

Query: 498 FGRAGGGHYTVYRRV----QDSDARLQDVSTEWFHVSDSEVHLVSEEEVLAAEATLLFYE 547
           FGR G GHYTVYR V    Q+ +    D    WF +SDSEV  VSE +VL AEA+LLFYE
Sbjct: 513 FGRTGSGHYTVYRSVRVFSQEEEEEDCDEDLSWFSISDSEVCRVSESDVLGAEASLLFYE 494

BLAST of CmoCh20G011300 vs. TAIR 10
Match: AT4G39370.3 (ubiquitin-specific protease 27 )

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-104
Identity = 235/553 (42.50%), Postives = 299/553 (54.07%), Query Frame = 0

Query: 18  AGLLSAAALFSVIRDGKLNNLGLLSWTAG--ASSSQNLRLLPGLQNLGNNCFFNVILQAL 77
           AGLL  A      + G   NL  L   +G    S  +  L+PGLQNLGNNCF NVILQAL
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSFLVPGLQNLGNNCFLNVILQAL 92

Query: 78  ASCVFFQPFLQKLMEEF--ELHEEMSDALPLTISLAALLEELSAISSERVVLSPRKIMLA 137
           ASC  F+ FLQ ++E+    L  E  + LPLT +L+ALL+EL  + S R V +PRK+M+ 
Sbjct: 93  ASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMVT 152

Query: 138 MAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCS-LVDVLASNGRIITSRSK- 197
           +  YA NFNLTSQQDAAEA LHL+SSL+EEI     P Q S L D+L S    + + S+ 
Sbjct: 153 LTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSEG 212

Query: 198 --SPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL-RGDSTIVF 257
                EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LPLSP+L  G   I+ 
Sbjct: 213 LHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIMS 272

Query: 258 GCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKMEIEELMKCDGQDSCGCDR 317
           GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA++ EIE+L  C G+D C C  
Sbjct: 273 GCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAETEIEKLRSCGGEDQCDCKT 332

Query: 318 LFDLYKVPWSNKFSFTLKQLSIAHCPKI-----------LCIHLKRVSTNMFGDVLKLQG 377
              L ++PWSN +S  LKQL IA  PK+           LCI ++R S NMF +  KL G
Sbjct: 333 SLHLQRMPWSNSYSHILKQLIIARFPKVFAYSLKLDVKLLCIQVQRASFNMF-EEFKLSG 392

Query: 378 HISFPLILDMSPFMLTGVEIKDIEKQLGKQHSSNKKPSHGLDVFRRQFDTTMLNYINGLT 437
           HI+FPL+L++S F  + + +                                        
Sbjct: 393 HIAFPLVLNLSLFTPSSIGV---------------------------------------- 452

Query: 438 GEDDYPTNLASGVPFCSKQLKPSAVETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSET 497
                  N+   +   S+  KP A +                                  
Sbjct: 453 -------NIEERIEMSSEYQKPEASKNHG------------------------------- 505

Query: 498 YLYRVVSVVQHFGRAGGGHYTVYRRV----QDSDARLQDVSTEWFHVSDSEVHLVSEEEV 547
            +YR+V+VV+HFGR G GHYTVYR V    Q+ +    D    WF +SDSEV  VSE +V
Sbjct: 513 -MYRLVTVVEHFGRTGSGHYTVYRSVRVFSQEEEEEDCDEDLSWFSISDSEVCRVSESDV 505

BLAST of CmoCh20G011300 vs. TAIR 10
Match: AT4G39370.2 (ubiquitin-specific protease 27 )

HSP 1 Score: 256.1 bits (653), Expect = 6.2e-68
Identity = 148/282 (52.48%), Postives = 182/282 (64.54%), Query Frame = 0

Query: 18  AGLLSAAALFSVIRDGKLNNLGLLSWTAG--ASSSQNLRLLPGLQNLGNNCFFNVILQAL 77
           AGLL  A      + G   NL  L   +G    S  +  L+PGLQNLGNNCF NVILQAL
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSFLVPGLQNLGNNCFLNVILQAL 92

Query: 78  ASCVFFQPFLQKLMEEF--ELHEEMSDALPLTISLAALLEELSAISSERVVLSPRKIMLA 137
           ASC  F+ FLQ ++E+    L  E  + LPLT +L+ALL+EL  + S R V +PRK+M+ 
Sbjct: 93  ASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMVT 152

Query: 138 MAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCS-LVDVLASNGRIITSRSK- 197
           +  YA NFNLTSQQDAAEA LHL+SSL+EEI     P Q S L D+L S    + + S+ 
Sbjct: 153 LTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSEG 212

Query: 198 --SPSELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPLSPVL-RGDSTIVF 257
                EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LPLSP+L  G   I+ 
Sbjct: 213 LHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIMS 272

Query: 258 GCTLLDCLKQFFAAEKVENYRCSSCWHDAGLKYLSSMGASKM 291
           GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA+++
Sbjct: 273 GCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAEV 314

BLAST of CmoCh20G011300 vs. TAIR 10
Match: AT1G32850.1 (ubiquitin-specific protease 11 )

HSP 1 Score: 66.2 bits (160), Expect = 9.1e-11
Identity = 59/191 (30.89%), Postives = 86/191 (45.03%), Query Frame = 0

Query: 54  RLLPGLQNLGNNCFFNVILQALA-SCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALL 113
           R L GLQNLGN CF N  LQ LA +    + FLQ    +      +     L I+   LL
Sbjct: 298 RGLGGLQNLGNTCFMNSTLQCLAHTPPIVEYFLQDYRSDINAKNPLGMRGELAIAFGELL 357

Query: 114 EELSAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQ 173
            +L   SS +  ++PR     +A +AP F+  +Q D+ E    LL  L E+++     P 
Sbjct: 358 RKL--WSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQEMLAFLLDGLHEDLNKVKRKPY 417

Query: 174 CSLVDVLASNGRIITSRSKSPSELERWQGH-------FLVPFDGILGSILTCQSCSSQIS 233
               D   S+GR     ++     E+W+ H        +  F G   S L C  C  +IS
Sbjct: 418 IEAKD---SDGRPDDEVAE-----EKWKYHKARNDSVIVDVFQGQYKSTLVCPDC-GKIS 477

Query: 234 LSFESFHNLPL 237
           ++F+ F  L L
Sbjct: 478 ITFDPFMYLSL 477


HSP 2 Score: 60.8 bits (146), Expect = 3.8e-09
Identity = 26/74 (35.14%), Postives = 44/74 (59.46%), Query Frame = 0

Query: 473 EPSETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEE 532
           E  ++YLY + ++  H+G  GGGHYT Y ++ D         T+W++  DS V  V+E E
Sbjct: 815 EDGQSYLYELYAISNHYGGLGGGHYTAYAKLMD--------ETKWYNFDDSRVSAVNESE 874

Query: 533 VLAAEATLLFYEKI 547
           +  + A +LFY+++
Sbjct: 875 IKTSAAYVLFYQRV 880

BLAST of CmoCh20G011300 vs. TAIR 10
Match: AT4G10590.2 (ubiquitin-specific protease 10 )

HSP 1 Score: 65.9 bits (159), Expect = 1.2e-10
Identity = 62/206 (30.10%), Postives = 89/206 (43.20%), Query Frame = 0

Query: 56  LPGLQNLGNNCFFNVILQALA-SCVFFQPFLQKLMEEFELHEEMSDALPLTISLAALLEE 115
           L GL NLGN CF N  LQ LA +    + FLQ   ++      +     L I+   LL++
Sbjct: 302 LAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGMCGELAIAFGDLLKK 361

Query: 116 LSAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEISDYNHPPQCS 175
           L   SS R  ++PR     +A +AP F+  +Q D+ E    LL  L E+++     P   
Sbjct: 362 L--WSSGRNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDGLHEDLNKVKRKPYIE 421

Query: 176 LVDVLASNGRIITSRSKSPSELERWQGH-------FLVPFDGILGSILTCQSCSSQISLS 235
           L D          SR       E W  H        +    G   S L C +C  +IS++
Sbjct: 422 LKD--------SDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPAC-GKISIT 481

Query: 236 FESFH--NLPLSPVLRGDSTI-VFGC 251
           F+ F   ++PL   L    T+ VF C
Sbjct: 482 FDPFMYLSVPLPSTLTRSMTVTVFYC 496


HSP 2 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 476 ETYLYRVVSVVQHFGRAGGGHYTVYRRVQDSDARLQDVSTEWFHVSDSEVHLVSEEEVLA 535
           ++YLY + +V  H+G  GGGHYT Y ++ D +        EW+H  DS V  V+E E+  
Sbjct: 832 QSYLYELYAVSNHYGGLGGGHYTAYAKLIDDN--------EWYHFDDSHVSSVNESEIKN 891

Query: 536 AEATLLFYEKI 547
           + A +LFY ++
Sbjct: 892 SAAYVLFYRRV 894

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FPS07.3e-10643.36Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana OX=3702 GN=UBP2... [more]
A4QNN31.0e-3026.45Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis OX=8364 GN=usp30 ... [more]
Q70CQ32.9e-3027.17Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens OX=9606 GN=USP30 PE=1 S... [more]
D3ZPG52.3e-2725.49Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus OX=10116 GN=Usp30 ... [more]
Q3UN045.2e-2725.68Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus OX=10090 GN=Usp30 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EIY60.0e+00100.00ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1I9D41.9e-30396.34ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A0A0LJP97.0e-26183.21USP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G298270 PE=3 SV... [more]
A0A5A7TSR62.8e-25482.31Ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3C6672.8e-25482.31ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo OX=3656 GN=LOC103497488... [more]
Match NameE-valueIdentityDescription
XP_022928102.10.0e+00100.00ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita moschata][more]
KAG6571374.18.1e-30497.07Ubiquitin carboxyl-terminal hydrolase 27, partial [Cucurbita argyrosperma subsp.... [more]
XP_022971724.14.0e-30396.34ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita maxima][more]
XP_023513383.15.4e-30095.79ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita pepo subsp. pepo][more]
XP_004148458.11.4e-26083.21ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G39370.15.2e-10743.36ubiquitin-specific protease 27 [more]
AT4G39370.31.4e-10442.50ubiquitin-specific protease 27 [more]
AT4G39370.26.2e-6852.48ubiquitin-specific protease 27 [more]
AT1G32850.19.1e-1130.89ubiquitin-specific protease 11 [more]
AT4G10590.21.2e-1030.10ubiquitin-specific protease 10 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001394Peptidase C19, ubiquitin carboxyl-terminal hydrolasePFAMPF00443UCHcoord: 58..543
e-value: 1.9E-41
score: 142.4
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 49..546
e-value: 1.5E-72
score: 246.5
NoneNo IPR availablePANTHERPTHR21646UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 49..545
NoneNo IPR availablePANTHERPTHR21646:SF24UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 49..545
IPR018200Ubiquitin specific protease, conserved sitePROSITEPS00972USP_1coord: 58..73
IPR028889Ubiquitin specific protease domainPROSITEPS50235USP_3coord: 57..546
score: 39.159111
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 56..545

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G011300.1CmoCh20G011300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0004843 thiol-dependent deubiquitinase