CmoCh20G011030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G011030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionterpene synthase 10-like
LocationCmo_Chr20: 10498882 .. 10499468 (-)
RNA-Seq ExpressionCmoCh20G011030
SyntenyCmoCh20G011030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAAGGTTTTCCATAACTTCAAGGATGAGAGAAAAAGCTTTAGCACAAGACACTGTCAAGATTTAAATGTTATGTTATCATTGTATGAAGCTTTGTTCTTATGTATAGAAGGTGAAAATATTCTAGAGACAACAAAACATTTTACAATAAACTATTTGGAAAAGTACATGAGATCATGCTATGATGAATATGAAACATCCACTATTAGACATGCATTGTGTTGGATGATGGAAGTCCCACATCGGCTAATTTAGGGAATGATCATGAGTTTATAAGTGAGGAATACTAACTCCATTGGTGTGAGGCCTTTTGGGGAAAGCCCAAAGCAAATCCATGAGAGCTTATGCTCAAAGTGAACAATATCATACCATTGTGGAGAGTAGTGTTCGTCTAACACATTGGAGCTTCCGTTACATTGGCAAATATCAAGATTGGATATAAGATGGTTCATTGATATCTATTAGAGAAAAGTGGATATGAACTCTATTCTTTTCGAAATTGCTAAATTAGATTTCAATCGAGTTCAATCTATCCACAAAAAAGATCTGAAATATGTATTAAGGACTATTATAAACCTATTTAG

mRNA sequence

ATGACAAAGGTTTTCCATAACTTCAAGGATGAGAGAAAAAGCTTTAGCACAAGACACTGTCAAGATTTAAATGTTATGTTATCATTGTATGAAGCTTTGTTCTTATGTATAGAAGGTGAAAATATTCTAGAGACAACAAAACATTTTACAATAAACTATTTGGAAAAATTTCAATCGAGTTCAATCTATCCACAAAAAAGATCTGAAATATGTATTAAGGACTATTATAAACCTATTTAG

Coding sequence (CDS)

ATGACAAAGGTTTTCCATAACTTCAAGGATGAGAGAAAAAGCTTTAGCACAAGACACTGTCAAGATTTAAATGTTATGTTATCATTGTATGAAGCTTTGTTCTTATGTATAGAAGGTGAAAATATTCTAGAGACAACAAAACATTTTACAATAAACTATTTGGAAAAATTTCAATCGAGTTCAATCTATCCACAAAAAAGATCTGAAATATGTATTAAGGACTATTATAAACCTATTTAG

Protein sequence

MTKVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSSSIYPQKRSEICIKDYYKPI
Homology
BLAST of CmoCh20G011030 vs. ExPASy Swiss-Prot
Match: Q93X23 (Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.7e-09
Identity = 30/55 (54.55%), Postives = 39/55 (70.91%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKF 58
           +VF++FKDER SF    C+D   MLSLYEA F  IEGENILE  + F+  +LE++
Sbjct: 170 EVFNSFKDERGSFKACLCEDTKGMLSLYEASFFLIEGENILEEARDFSTKHLEEY 224

BLAST of CmoCh20G011030 vs. ExPASy Swiss-Prot
Match: Q50L36 (Isoprene synthase, chloroplastic OS=Populus alba OX=43335 GN=ISPS PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSSSI 62
           + F  FKD+  +F     +D+  +LSLYEA FL +EGENIL+  K F I++L++     I
Sbjct: 168 EAFSGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKI 227

BLAST of CmoCh20G011030 vs. ExPASy Swiss-Prot
Match: Q7XAS7 (Isoprene synthase, chloroplastic OS=Populus tremuloides OX=3693 GN=ISPS PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSSSI 62
           + F  FKD+  +F     +D+  +LSLYEA FL +EGENIL+  K F I++L++     I
Sbjct: 168 EAFSGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKI 227

BLAST of CmoCh20G011030 vs. ExPASy Swiss-Prot
Match: Q6PWU2 ((-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.7e-06
Identity = 23/53 (43.40%), Postives = 33/53 (62.26%), Query Frame = 0

Query: 4   VFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEK 57
           VF  FKD+  SF    C+D+  ML LYEA +LC++GE+ +E  + F   +L K
Sbjct: 163 VFSRFKDDTGSFKACLCEDMKGMLCLYEASYLCVQGESTMEQARDFAHRHLGK 215

BLAST of CmoCh20G011030 vs. ExPASy Swiss-Prot
Match: B3TPQ7 (Alpha-terpineol synthase, chloroplastic OS=Magnolia grandiflora OX=3406 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.2e-06
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 0

Query: 4   VFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYL 55
           VF+ F D+  SF    C D+  MLSLYEA +L +EGE  L+  K FT  +L
Sbjct: 167 VFNRFMDDSSSFKASLCNDVKGMLSLYEASYLALEGETTLDEAKAFTYRHL 217

BLAST of CmoCh20G011030 vs. ExPASy TrEMBL
Match: A0A6J1I6L4 (terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470417 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.9e-16
Identity = 45/58 (77.59%), Postives = 48/58 (82.76%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VFHNFKDE K+FSTR  QDLN MLSLYEA FLCIE ENILE  KHFTINYLEK+  S
Sbjct: 128 EVFHNFKDETKNFSTRLYQDLNGMLSLYEASFLCIESENILEAAKHFTINYLEKYMRS 185

BLAST of CmoCh20G011030 vs. ExPASy TrEMBL
Match: A0A6J1I2T8 (terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-14
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+NFKDE K+FSTR  +DLN MLSLYEA FLCIEGENILE  KHFTIN+LEK+  S
Sbjct: 160 EVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRS 217

BLAST of CmoCh20G011030 vs. ExPASy TrEMBL
Match: A0A6J1EJW9 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-13
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+NFKDE K+FS +  +DLN +LSLYEA FLCIEGENILET KHFTINYLE +  S
Sbjct: 161 EVFNNFKDETKNFSKQLYEDLNGILSLYEASFLCIEGENILETAKHFTINYLENYMRS 218

BLAST of CmoCh20G011030 vs. ExPASy TrEMBL
Match: A0A6J1EJ26 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434910 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-13
Identity = 41/58 (70.69%), Postives = 47/58 (81.03%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+NF DE K+F+T   QDLN MLSLYEA FLCIEGENILE  K+FTINYLEK+  S
Sbjct: 161 EVFNNFMDETKNFNTGLYQDLNGMLSLYEASFLCIEGENILEAAKYFTINYLEKYMRS 218

BLAST of CmoCh20G011030 vs. ExPASy TrEMBL
Match: A0A6J1EMY1 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-12
Identity = 40/58 (68.97%), Postives = 47/58 (81.03%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+N+KDE K+FSTR  +DLN MLSLYE  FL IEGENILE  KHFTINYL+K+  S
Sbjct: 161 EVFNNYKDEMKNFSTRLYEDLNGMLSLYEVSFLRIEGENILEAAKHFTINYLKKYMRS 218

BLAST of CmoCh20G011030 vs. NCBI nr
Match: XP_023512338.1 (terpene synthase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 94.0 bits (232), Expect = 6.3e-16
Identity = 45/58 (77.59%), Postives = 49/58 (84.48%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           ++FHNFKDE K+FSTR  QDLN MLSLYEA FLCIEGENILE  KHFTINYLEK+  S
Sbjct: 161 EIFHNFKDETKNFSTRLYQDLNGMLSLYEASFLCIEGENILEEAKHFTINYLEKYMRS 218

BLAST of CmoCh20G011030 vs. NCBI nr
Match: XP_022971750.1 (terpene synthase 10-like [Cucurbita maxima])

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-15
Identity = 45/58 (77.59%), Postives = 48/58 (82.76%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VFHNFKDE K+FSTR  QDLN MLSLYEA FLCIE ENILE  KHFTINYLEK+  S
Sbjct: 128 EVFHNFKDETKNFSTRLYQDLNGMLSLYEASFLCIESENILEAAKHFTINYLEKYMRS 185

BLAST of CmoCh20G011030 vs. NCBI nr
Match: XP_022971747.1 (terpene synthase 10-like [Cucurbita maxima])

HSP 1 Score: 88.6 bits (218), Expect = 2.7e-14
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+NFKDE K+FSTR  +DLN MLSLYEA FLCIEGENILE  KHFTIN+LEK+  S
Sbjct: 160 EVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRS 217

BLAST of CmoCh20G011030 vs. NCBI nr
Match: XP_023512342.1 (terpene synthase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 88.2 bits (217), Expect = 3.5e-14
Identity = 42/58 (72.41%), Postives = 49/58 (84.48%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+N+ DE K+FSTR  +DLN MLSLYEA FLCIEGENILET KHFTINYL+K+  S
Sbjct: 161 EVFNNYNDEMKNFSTRLYEDLNGMLSLYEASFLCIEGENILETAKHFTINYLKKYMRS 218

BLAST of CmoCh20G011030 vs. NCBI nr
Match: XP_022928104.1 (terpene synthase 10-like [Cucurbita moschata])

HSP 1 Score: 85.5 bits (210), Expect = 2.2e-13
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0

Query: 3   KVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKFQSS 61
           +VF+NFKDE K+FS +  +DLN +LSLYEA FLCIEGENILET KHFTINYLE +  S
Sbjct: 161 EVFNNFKDETKNFSKQLYEDLNGILSLYEASFLCIEGENILETAKHFTINYLENYMRS 218

BLAST of CmoCh20G011030 vs. TAIR 10
Match: AT4G15870.1 (terpene synthase 1 )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 28/80 (35.00%), Postives = 37/80 (46.25%), Query Frame = 0

Query: 2   TKVFHNFKDERKSFSTRHCQDLNVMLSLYEALFLCIEGENILETTKHFTINYLEKF---- 61
           T VF  FK E + F      D+  M+SLYEA  L    E+ILE    FT   LE      
Sbjct: 182 TDVFKRFKGENEKFMESITSDVKGMVSLYEAAHLRTTREDILEEALSFTTRNLESLARAG 241

Query: 62  -QSSSIYPQKRSEICIKDYY 77
             S  I  + R+ +C+  +Y
Sbjct: 242 ASSPHILMRIRNALCMPQHY 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93X232.7e-0954.55Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1[more]
Q50L362.1e-0641.67Isoprene synthase, chloroplastic OS=Populus alba OX=43335 GN=ISPS PE=1 SV=1[more]
Q7XAS72.1e-0641.67Isoprene synthase, chloroplastic OS=Populus tremuloides OX=3693 GN=ISPS PE=3 SV=... [more]
Q6PWU24.7e-0643.40(-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
B3TPQ76.2e-0647.06Alpha-terpineol synthase, chloroplastic OS=Magnolia grandiflora OX=3406 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1I6L48.9e-1677.59terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470417 PE=4 SV=1[more]
A0A6J1I2T81.3e-1474.14terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1[more]
A0A6J1EJW91.1e-1370.69terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1[more]
A0A6J1EJ264.1e-1370.69terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434910 PE=4 SV=1[more]
A0A6J1EMY11.2e-1268.97terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023512338.16.3e-1677.59terpene synthase 10-like [Cucurbita pepo subsp. pepo][more]
XP_022971750.11.8e-1577.59terpene synthase 10-like [Cucurbita maxima][more]
XP_022971747.12.7e-1474.14terpene synthase 10-like [Cucurbita maxima][more]
XP_023512342.13.5e-1472.41terpene synthase 10-like [Cucurbita pepo subsp. pepo][more]
XP_022928104.12.2e-1370.69terpene synthase 10-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G15870.12.0e-0435.00terpene synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 3..60
e-value: 5.6E-14
score: 52.3
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 1..75
e-value: 3.0E-15
score: 58.4
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 3..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G011030.1CmoCh20G011030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006368 transcription elongation from RNA polymerase II promoter
cellular_component GO:0032044 DSIF complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0003729 mRNA binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity