CmoCh20G009900 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G009900
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	cytochrome P450 81D1-like
Location	Cmo_Chr20: 5647937 .. 5650206 (-)
RNA-Seq Expression	CmoCh20G009900
Synteny	CmoCh20G009900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTCCAGAAAACTCCATGGATATTCTCTTGCTTTCTATTTCTCTCTTTATATTTCCTCTTCTCTTTGCCTTCAATTTTGTGTTGAAACCACGTCGCTTGAAACTCCCACCAGGCCCTCTCTTTAGTCTTCCACTACTTGGCCATCTCCATCTCCTAAACCATCCCATCCATCGAACACTCACCAACCTTTCTCAAAAATACGGTCATGTCTTCTCCCTCCGATTTGGTTCTCGTCTCGTTGTTGTTATCTCTTCTCCTTCGGCCGTCCAAGAATGCTTCACAAAAAACGATATTGTTCTTGCAAACCGCCCATTTTTGAACTCTACAAAGTACCTAAACTACAACCATACCGTCATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCGGATAAGCTCCCTTGAGATCTTCTCAACAAATCGCCTCAATTTGTTCTTGGGAATTCGGGAAGAGGAAATCAAATGTTTGCTATGTAAATTATGTGGGGATTCATTGGAAGATCGATTCAGGGTCGTGGAATTGGAGCCCATGTTGCTTGATCTTATGTTCAATATTGTTACGAGAATGGTGGCTGGGAAGAGGTTTTATGAGCGACATGACAAGTTTAGAGAGATGGTGACACAAATTATGGCTCACAGTGGAGCAACCAATCCAGGAGATTTCATACCCTTATGGAGATTCATTGATCCAAGTGGTTTTGAGAAGAGGGTAAAGAAGCTTGGGAAGACATCTGATGAACTCCTTCAAGAATTAGTGGATGAAATTCGAGATAAAAATGATGGAGGGAACACTATGATTCACCGTTTACTTAGCTTGCAGAATACCGAGCCTGAATATTACAGCGATCAAATTATCAAAGGATTGATTCAAGTATGATTAACTCCTACCCTTTTGTTAATGATTTCTTCTTAATTTGTTGTGTTCTATGAATGACTCTCTACATGTTATAATGTTGTCACTAAATTAGCTAACAAATTAGCCAACATGGGACTCCCTCCCAACCATATTCCCCTTGAACAAAGTACACCATAGAGCCTCCCCTGAGGCCTACGGAGCCCTCGAACAGCCTTTCCTTAATTGAGACTCGAATCTTTACGGCCTCCCCTTAATCGAGGCTCGAATCCTTACAGCCTCCCCTGAAGCCTACGGAGCCCTAAAAAAGTTTCCCCTTAAATCAAGGCTCGACTCCTTTCTCTGAAGCCATCAAACAAAATACACCATAGAGCCTCCCGTGAGGTTTACGGGGCCCCTCAAACAGCCTCTCCCCTTAATCGAGGCTCGACTCCTTTCTCCGGAGCCCTCGAACAAAGTACACTATTTGTTTGACACTTGAGTAACTTTTGACTACACTTTTGAGGCTCACAACTTCTTTGTTCGACATTTGAGAATTCTATTGACATGGCTAAGTTAAGGGCATGACTTTAATACCATGTTAGAAATAACAACACGCCATAATGGTATGATGTTAACCACTTTGAGCATAAGCTCTCATGGCTTTGTTTTGTGCTTCCCCAAGAGGCCTTAGACCAATGAAGATGTATTCTTTACTAACTCATGATTATCCCCTAAATTAGTCAATGTGGAACTCCTTCCAACCATCTTCAATATTATGAAAGTGTTTGGTTGTTGCAGGATATATTATTAGCCGGGATTGACACGTTAGCTGTGACATTGGAGTGGACAGTATCACAATTGCTCAACCATCCAGAGGTGTTAGAGAAAGCAAGAGCTGAGATAGACAGTTCGATTGGGTACGAGCGTTTAGTTAACGAATCTGATCTGCCGAGTTTGAGTTATGTTCAAGGAATAATCTCTGAGACTTTGCGGCTTCACCCAGCTGCTCCTCTTCTTTTGCCGCATTGTGCATTTGAAGAATGCAAAATAGAAGGATATGATGTTCCTCGTGATACGATAATATTCATCAATGCTTGGGCCATGCATAGAGACCCGAATCTATGGGAAGATGCTACCAGCTTTAAACCTGAAAGACATGCAAATGGGATGAAGAATGGCGCAGAGTCATACAAACTGATACCATTTGGGTTGGGGAGGAGAGCTTGTCCTGGTTTGGGAATGGCGCAACGAGTGGCTGGCTTGACTTTAGCATCGTTGATACAAGGCTTTGAATGGGAAAGATTGAACAACTCTTTAGTTGACATGAGTGAAGGTAAAGGGATTACAATGCCCAAAGCTCAACCCTTGGTAGCCAAGTGTAAGCCACGCCCCATCATGAAACTCGTTTATGCATGTACCAATTGA

mRNA sequence

AAATTCCAGAAAACTCCATGGATATTCTCTTGCTTTCTATTTCTCTCTTTATATTTCCTCTTCTCTTTGCCTTCAATTTTGTGTTGAAACCACGTCGCTTGAAACTCCCACCAGGCCCTCTCTTTAGTCTTCCACTACTTGGCCATCTCCATCTCCTAAACCATCCCATCCATCGAACACTCACCAACCTTTCTCAAAAATACGGTCATGTCTTCTCCCTCCGATTTGGTTCTCGTCTCGTTGTTGTTATCTCTTCTCCTTCGGCCGTCCAAGAATGCTTCACAAAAAACGATATTGTTCTTGCAAACCGCCCATTTTTGAACTCTACAAAGTACCTAAACTACAACCATACCGTCATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCGGATAAGCTCCCTTGAGATCTTCTCAACAAATCGCCTCAATTTGTTCTTGGGAATTCGGGAAGAGGAAATCAAATGTTTGCTATGTAAATTATGTGGGGATTCATTGGAAGATCGATTCAGGGTCGTGGAATTGGAGCCCATGTTGCTTGATCTTATGTTCAATATTGTTACGAGAATGGTGGCTGGGAAGAGGTTTTATGAGCGACATGACAAGTTTAGAGAGATGGTGACACAAATTATGGCTCACAGTGGAGCAACCAATCCAGGAGATTTCATACCCTTATGGAGATTCATTGATCCAAGTGGTTTTGAGAAGAGGGTAAAGAAGCTTGGGAAGACATCTGATGAACTCCTTCAAGAATTAGTGGATGAAATTCGAGATAAAAATGATGGAGGGAACACTATGATTCACCGTTTACTTAGCTTGCAGAATACCGAGCCTGAATATTACAGCGATCAAATTATCAAAGGATTGATTCAATGGACAGTATCACAATTGCTCAACCATCCAGAGGTGTTAGAGAAAGCAAGAGCTGAGATAGACAGTTCGATTGGGTACGAGCGTTTAGTTAACGAATCTGATCTGCCGAGTTTGAGTTATGTTCAAGGAATAATCTCTGAGACTTTGCGGCTTCACCCAGCTGCTCCTCTTCTTTTGCCGCATTGTGCATTTGAAGAATGCAAAATAGAAGGATATGATGTTCCTCGTGATACGATAATATTCATCAATGCTTGGGCCATGCATAGAGACCCGAATCTATGGGAAGATGCTACCAGCTTTAAACCTGAAAGACATGCAAATGGGATGAAGAATGGCGCAGAGTCATACAAACTGATACCATTTGGGTTGGGGAGGAGAGCTTGTCCTGGTTTGGGAATGGCGCAACGAGTGGCTGGCTTGACTTTAGCATCGTTGATACAAGGCTTTGAATGGGAAAGATTGAACAACTCTTTAGTTGACATGAGTGAAGGTAAAGGGATTACAATGCCCAAAGCTCAACCCTTGGTAGCCAAGTGTAAGCCACGCCCCATCATGAAACTCGTTTATGCATGTACCAATTGA

Coding sequence (CDS)

ATGGATATTCTCTTGCTTTCTATTTCTCTCTTTATATTTCCTCTTCTCTTTGCCTTCAATTTTGTGTTGAAACCACGTCGCTTGAAACTCCCACCAGGCCCTCTCTTTAGTCTTCCACTACTTGGCCATCTCCATCTCCTAAACCATCCCATCCATCGAACACTCACCAACCTTTCTCAAAAATACGGTCATGTCTTCTCCCTCCGATTTGGTTCTCGTCTCGTTGTTGTTATCTCTTCTCCTTCGGCCGTCCAAGAATGCTTCACAAAAAACGATATTGTTCTTGCAAACCGCCCATTTTTGAACTCTACAAAGTACCTAAACTACAACCATACCGTCATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCGGATAAGCTCCCTTGAGATCTTCTCAACAAATCGCCTCAATTTGTTCTTGGGAATTCGGGAAGAGGAAATCAAATGTTTGCTATGTAAATTATGTGGGGATTCATTGGAAGATCGATTCAGGGTCGTGGAATTGGAGCCCATGTTGCTTGATCTTATGTTCAATATTGTTACGAGAATGGTGGCTGGGAAGAGGTTTTATGAGCGACATGACAAGTTTAGAGAGATGGTGACACAAATTATGGCTCACAGTGGAGCAACCAATCCAGGAGATTTCATACCCTTATGGAGATTCATTGATCCAAGTGGTTTTGAGAAGAGGGTAAAGAAGCTTGGGAAGACATCTGATGAACTCCTTCAAGAATTAGTGGATGAAATTCGAGATAAAAATGATGGAGGGAACACTATGATTCACCGTTTACTTAGCTTGCAGAATACCGAGCCTGAATATTACAGCGATCAAATTATCAAAGGATTGATTCAATGGACAGTATCACAATTGCTCAACCATCCAGAGGTGTTAGAGAAAGCAAGAGCTGAGATAGACAGTTCGATTGGGTACGAGCGTTTAGTTAACGAATCTGATCTGCCGAGTTTGAGTTATGTTCAAGGAATAATCTCTGAGACTTTGCGGCTTCACCCAGCTGCTCCTCTTCTTTTGCCGCATTGTGCATTTGAAGAATGCAAAATAGAAGGATATGATGTTCCTCGTGATACGATAATATTCATCAATGCTTGGGCCATGCATAGAGACCCGAATCTATGGGAAGATGCTACCAGCTTTAAACCTGAAAGACATGCAAATGGGATGAAGAATGGCGCAGAGTCATACAAACTGATACCATTTGGGTTGGGGAGGAGAGCTTGTCCTGGTTTGGGAATGGCGCAACGAGTGGCTGGCTTGACTTTAGCATCGTTGATACAAGGCTTTGAATGGGAAAGATTGAACAACTCTTTAGTTGACATGAGTGAAGGTAAAGGGATTACAATGCCCAAAGCTCAACCCTTGGTAGCCAAGTGTAAGCCACGCCCCATCATGAAACTCGTTTATGCATGTACCAATTGA

Protein sequence

MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMFNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMKLVYACTN

Homology

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BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.4e-145
Identity = 262/500 (52.40%), Postives = 350/500 (70.00%), Query Frame = 0


Query: 3   ILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKY 62
           ++LLSISL +FP        +  RR  LPP P  +LP++GHLHL+   +HR+L +LS+KY
Sbjct: 13  VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72

Query: 63  GHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYGEH 122
           G VFSL+ G+RLV+V+SSP+A +ECFTKNDIV ANRP     KY+ YN+T M  SPYGEH
Sbjct: 73  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132

Query: 123 WRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMFN 182
           WRNLRR++++EIFS   LN FL IRE+E+K LL  L   S +D F  VE++  L +L FN
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQD-FGKVEMKSKLSELSFN 192

Query: 183 IVTRMVAGKRFY------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVK 242
           +  RMVAGKR++      +    FR ++ ++  H+GA+NPGDF+P  R+ID   +EK+V 
Sbjct: 193 VTMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 252

Query: 243 KLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLI--------- 302
           K+ +  D  LQ L+ E R  N    TMI  LLSLQ ++PEYY+DQIIKG+I         
Sbjct: 253 KISQEMDAFLQRLIHESR-INKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTD 312

Query: 303 ------QWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHP 362
                 +W +S LLNHPE LEKAR EI++ +G  RL+ E DLP L+Y+  IISET RL P
Sbjct: 313 TSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCP 372

Query: 363 AAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGA 422
           AAP+L+PH + ++CK++GYDVP+ TI+ +NAWA+HRDP  W++ T FKPERH  G++   
Sbjct: 373 AAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH-GGVE--L 432

Query: 423 ESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 482
           E  KL+PFG+GRR+CPG G+AQRV GLTL +LIQ FEW+R+  + +DM+EG G+TMPKAQ
Sbjct: 433 EPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQ 492

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BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 498.4 bits (1282), Expect = 8.9e-140
Identity = 255/503 (50.70%), Postives = 346/503 (68.79%), Query Frame = 0


Query: 1   MDIL-LLSISLFIFPLLFAFNFVLKPRRLK-LPPGPLFSLPLLGHLHLLNHPIHRTLTNL 60
           M+IL LLS S+F   L F FN V++ R+ K LPPGP  SLP++G+LH L  P+HRT   L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SQKYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSP 120
           S+KYGHVFSL FGSRLVVV+SS S  Q+CFTKND+VLANRP   S KY+ YN+T +  + 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 YGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLD 180
           YGEHWRNLRRI++L++ S +R+N F GIR +E + L+ +L  DS    F  +EL   L D
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDS-STNFAEMELSSRLYD 180

Query: 181 LMFNIVTRMVAGKRFY---------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPS 240
           + FN + RM++GKR+Y         +   +FR+MV++++  SGA N  DF+PL RF+D  
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 241 GFEKRVKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGL--- 300
             EKR+K +   +D  L+ L++E R K +  NTMI  LL+LQ+++PEYY+DQIIKGL   
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALA 300

Query: 301 ------------IQWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 360
                       ++W++S LLNHPEVL+K + E+D+ +G +RLV+ESDLP L+Y++ +I+
Sbjct: 301 MLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVIN 360

Query: 361 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 420
           ETLRL+  APLLLPH   +EC I GY VP+DTI+ INAWA+HRDP LW +AT+FKPER  
Sbjct: 361 ETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPER-- 420

Query: 421 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 478
              +   E  KLI FG+GRRACPG G+A R   +TLA LIQ F+W+ +N   +D++E  G
Sbjct: 421 --FEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDG 480

[Close]

BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.5e-134
Identity = 254/499 (50.90%), Postives = 327/499 (65.53%), Query Frame = 0


Query: 5   LLSISLFIFPLLFAFNFVLK--PRRLK-LPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQK 64
           L   SL     +     +LK   RRLK LPPGP  ++P++G+LH L HP+HRT T LSQ 
Sbjct: 2   LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61

Query: 65  YGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYGE 124
           YG +FSL FGSRLVVV+SSPS   ECFTKNDI+LANRP   + KY+ YN+T +  + YG+
Sbjct: 62  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121

Query: 125 HWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLED--RFRVVELEPMLLDL 184
           HWRNLRRI+++++ S NRLN FLG+R +E   L+ KL  D + +   F  VEL P L ++
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 181

Query: 185 MFNIVTRMVAGKRFY---------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSG 244
            FN + RM++GKR+Y         E   +FRE+++++M+  GA N GDF+PL R +D   
Sbjct: 182 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 241

Query: 245 FEKRVKKLGKTSDELLQELVDEIRDKN--DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ 304
            EKR K++ K S+  L+ L++E R  N    G TMI  LL L  ++PEYYSD +IKGLIQ
Sbjct: 242 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 301

Query: 305 ---------------WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGII 364
                          W +S+LLNHPEVL+KA+ E+D+ IG  +LV+E DL  L Y+Q II
Sbjct: 302 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 361

Query: 365 SETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERH 424
           SETLRLHP APLLLPH + E+C I  ++VP+DTII  N W +HRDP  W DA SFKPER 
Sbjct: 362 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER- 421

Query: 425 ANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGK 473
               +   E  K++ FGLGRRACPGL +AQR  G T+  LIQ FEWER +   +DM EGK
Sbjct: 422 ---FEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGK 481

[Close]

BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.6e-133
Identity = 245/497 (49.30%), Postives = 333/497 (67.00%), Query Frame = 0


Query: 6   LSISLFIFPLLFAFNFVLKPRRLK-LPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKYGH 65
           L  SLF   + F    + + R+ K LPPGP  SLP++G+LH L  P++RT   L++KYG+
Sbjct: 7   LCYSLFYLSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTEKYGN 66

Query: 66  VFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYGEHWR 125
           V SL FGSRLVVV+SS S  QECFTKND+VLANRP   S KY+ YN+T +  + YGEHWR
Sbjct: 67  VISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWR 126

Query: 126 NLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMFNIV 185
           NLRRI+SL++ S +R+N F  IR +E + L+ KL  DS   +F  VEL     D+ FN +
Sbjct: 127 NLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDS-STKFAEVELTFRFFDMTFNNI 186

Query: 186 TRMVAGKRFY---------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRV 245
            RM++GKR+Y         +   +FR+MV++++  SGA N  DF+PL +F+D    EKRV
Sbjct: 187 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 246

Query: 246 KKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGL--------- 305
           K++G+ +D  L  L+ E R K +  NTMI  LL++Q ++PEYY+D IIKGL         
Sbjct: 247 KRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGT 306

Query: 306 ------IQWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLH 365
                 ++WT+S +LN+PEVL+K R E+D+ +G +RLV+ESDLP L+Y++ +I ETLRL+
Sbjct: 307 DSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLY 366

Query: 366 PAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNG 425
             APLLLPH   +EC + GY VPRDTI+ INAWA+HRDP  W +AT+FKPER        
Sbjct: 367 TPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPER----FDKK 426

Query: 426 AESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKA 478
            E  K+I FG+GRRACPG G+A R   +TLA L+Q F+W+R+N+  +DMSE  G TM K 
Sbjct: 427 GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKL 486

[Close]

BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 8.9e-132
Identity = 251/499 (50.30%), Postives = 335/499 (67.13%), Query Frame = 0


Query: 3   ILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKY 62
           ++L SI   IF L+ +F F LKP++  LPP P   LP++GHL LL  PIHRTL + S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----VFSLRFGSRLVVVISSPS-AVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSV 122
            H     V SLR GSRLV V+SS   A +ECF KND+VLANRP +   K++ YN+T M  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPML 182
           +PYG+HWRNLRR+ ++EIFST+RLN FL +R +E++ L+ +L       +  VVEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPML 187

Query: 183 LDLMFNIVTRMVAGKRFY-------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPS 242
           +DL FN + RM+ GKR+Y       E   + R++V  + A++ + N  D++P+ R    S
Sbjct: 188 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 247

Query: 243 GFEKRVKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLI-- 302
            +E RVKKLG+ +D+ LQ L+D+ R + + G TMI  LL LQ ++ EYY+DQIIKG+I  
Sbjct: 248 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307

Query: 303 -------------QWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 362
                        +W +S LLNHP+V+ KAR EID+ +G +RL+ E+DL  L Y++ I+ 
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 367

Query: 363 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 422
           ETLRLHPA PLL+PH A E+CKI  YD+PR T + +NAWA+HRDPN W+D  SFKPER  
Sbjct: 368 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER-- 427

Query: 423 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 474
              +   E+ KL+ FGLGRRACPG G+AQR+ GL L SLIQ FEWER+ N  VDM EG G
Sbjct: 428 --FEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487

[Close]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 5.5e-278
Identity = 481/496 (96.98%), Postives = 481/496 (96.98%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240
           FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK
Sbjct: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240

Query: 241 TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ------------ 300
           TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ            
Sbjct: 241 TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAV 300

Query: 301 ---WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL 360
              WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL
Sbjct: 301 TLEWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL 360

Query: 361 LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK 420
           LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK
Sbjct: 361 LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK 420

Query: 421 LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480
           LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA
Sbjct: 421 LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480

Query: 481 KCKPRPIMKLVYACTN 482
           KCKPRPIMKLVYACTN
Sbjct: 481 KCKPRPIMKLVYACTN 496

[Close]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 446/497 (89.74%), Postives = 458/497 (92.15%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFAFNFVLKP RL LPPGPL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDI LANRP LNS KYL+YNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLNLFLG+R+EEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240
           FNIV +MVAGK+ +E H  KF+EMVTQIMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ----------- 300
           KTSDELLQELVDEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ           
Sbjct: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300

Query: 301 ----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
               W +SQLLN+PEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYACTN 482
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYESGN 497

[Close]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.6e-248
Identity = 435/497 (87.53%), Postives = 451/497 (90.74%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFA NFVLKP RLKLPP PL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFASNFVLKPHRLKLPPTPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLV+VISSPSAVQECFTKNDI LANRP LNS KYL+YN+TVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVIVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNYTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN FLG+R EEIKCLLCKLCG+ LEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIVSLEIFSTNRLNFFLGVRGEEIKCLLCKLCGNLLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240
           FNIV RM+AGK+ +E H  KF+EMVT IMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVMRMLAGKKSFEGHSYKFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ----------- 300
           KTSDELLQEL+DEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ           
Sbjct: 241 KTSDELLQELLDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLV 300

Query: 301 ----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
               W +SQLLN+PEVLEKARAEIDSSIGYERLVNE+DLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWALSQLLNNPEVLEKARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCA EECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCASEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYACTN 482
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYESGN 497

[Close]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.3e-206
Identity = 359/493 (72.82%), Postives = 411/493 (83.37%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L +  LLFAFNF+  P RL LPP PLFSLPL+GH HLL HP+HR+L  LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVVVISSPSAVQECFTKNDI+LANRP L++ KYL YNHT ++VSPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN+F+GIRE E+K LL KLCGD  E+ FR VELEPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEE-FREVELEPMLSDLT 180

Query: 181 FNIVTRMVAGKRFYER--HDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKL 240
           FN+V  MV G+RFY+     KFR++VTQIMAH G++NPGDFIPLW ++DP+GF+KR+  L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 GKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ---------- 300
            +  DELLQEL+DE RD  DGGNTMIH LL LQ  EPEYYSD IIKGLIQ          
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 -----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
                W +S LLN+P+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ IN WA+HRDPNLWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERH--GDTSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 477
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

[Close]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL
Match: A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.3e-206
Identity = 357/493 (72.41%), Postives = 414/493 (83.98%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L +  LLFAFNF+  P RL LPP PLFSLPL+GH HLL HP+HR+L NLSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVV+ISSPSAVQECFTKNDI+LANRP L++ KYL YNHT +++SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFST+RLNLFLGIR+ E+K LL KLCGD LE+ FR VE+EPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEE-FREVEVEPMLSDLT 180

Query: 181 FNIVTRMVAGKRFYER--HDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKL 240
           FN+V  MV G+RFY+     KFR++VTQIMAH G++NPGDFIPLW ++DP+GF+KR+  L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 GKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ---------- 300
            +  DELLQEL+DE RD  DGGN+MIH LL LQ  EPEYYSD IIKGLIQ          
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 -----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
                W +S LLN+P+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ INAWA+HRDP+LWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERH--GDSSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 477
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

[Close]

BLAST of CmoCh20G009900 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 966.1 bits (2496), Expect = 1.1e-277
Identity = 481/496 (96.98%), Postives = 481/496 (96.98%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240
           FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK
Sbjct: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240

Query: 241 TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ------------ 300
           TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ            
Sbjct: 241 TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAV 300

Query: 301 ---WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL 360
              WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL
Sbjct: 301 TLEWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL 360

Query: 361 LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK 420
           LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK
Sbjct: 361 LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK 420

Query: 421 LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480
           LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA
Sbjct: 421 LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480

Query: 481 KCKPRPIMKLVYACTN 482
           KCKPRPIMKLVYACTN
Sbjct: 481 KCKPRPIMKLVYACTN 496

[Close]

BLAST of CmoCh20G009900 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 926.8 bits (2394), Expect = 7.7e-266
Identity = 456/496 (91.94%), Postives = 471/496 (94.96%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFIFPLLFAFNFVLKP RL LPP PLF LP++GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPHRLNLPPSPLFCLPIIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRP LNS KY++YNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPLLNSAKYIDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240
           FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK
Sbjct: 181 FNIVTRMVAGKRFYERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGK 240

Query: 241 TSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ------------ 300
           T DE+L+ELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ            
Sbjct: 241 TCDEILRELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAV 300

Query: 301 ---WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPL 360
              W +SQLLNHPEVLEKARAEID+SIGYERLVNE DLP+LSYVQGIISET+RL+PAAPL
Sbjct: 301 TLEWALSQLLNHPEVLEKARAEIDNSIGYERLVNEVDLPNLSYVQGIISETMRLNPAAPL 360

Query: 361 LLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYK 420
           L+PHCAFEECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGA+SYK
Sbjct: 361 LVPHCAFEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGADSYK 420

Query: 421 LIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480
           LIPFGLGRRACPG+GMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA
Sbjct: 421 LIPFGLGRRACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVA 480

Query: 481 KCKPRPIMKLVYACTN 482
           KCKPRPIMKLVYAC+N
Sbjct: 481 KCKPRPIMKLVYACSN 496

[Close]

BLAST of CmoCh20G009900 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.0 bits (2301), Expect = 4.7e-255
Identity = 446/497 (89.74%), Postives = 458/497 (92.15%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFAFNFVLKP RL LPPGPL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDI LANRP LNS KYL+YNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLNLFLG+R+EEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240
           FNIV +MVAGK+ +E H  KF+EMVTQIMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ----------- 300
           KTSDELLQELVDEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ           
Sbjct: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300

Query: 301 ----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
               W +SQLLN+PEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYACTN 482
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYESGN 497

[Close]

BLAST of CmoCh20G009900 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 866.7 bits (2238), Expect = 9.5e-248
Identity = 435/497 (87.53%), Postives = 451/497 (90.74%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFA NFVLKP RLKLPP PL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFASNFVLKPHRLKLPPTPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLV+VISSPSAVQECFTKNDI LANRP LNS KYL+YN+TVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVIVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNYTVMSVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN FLG+R EEIKCLLCKLCG+ LEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIVSLEIFSTNRLNFFLGVRGEEIKCLLCKLCGNLLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240
           FNIV RM+AGK+ +E H  KF+EMVT IMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVMRMLAGKKSFEGHSYKFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ----------- 300
           KTSDELLQEL+DEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ           
Sbjct: 241 KTSDELLQELLDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLV 300

Query: 301 ----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
               W +SQLLN+PEVLEKARAEIDSSIGYERLVNE+DLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWALSQLLNNPEVLEKARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCA EECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCASEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYACTN 482
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYESGN 497

[Close]

BLAST of CmoCh20G009900 vs. NCBI nr
Match: XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])

HSP 1 Score: 727.6 bits (1877), Expect = 6.9e-206
Identity = 359/493 (72.82%), Postives = 411/493 (83.37%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L +  LLFAFNF+  P RL LPP PLFSLPL+GH HLL HP+HR+L  LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVVVISSPSAVQECFTKNDI+LANRP L++ KYL YNHT ++VSPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN+F+GIRE E+K LL KLCGD  E+ FR VELEPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEE-FREVELEPMLSDLT 180

Query: 181 FNIVTRMVAGKRFYER--HDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKL 240
           FN+V  MV G+RFY+     KFR++VTQIMAH G++NPGDFIPLW ++DP+GF+KR+  L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 GKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQ---------- 300
            +  DELLQEL+DE RD  DGGNTMIH LL LQ  EPEYYSD IIKGLIQ          
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 -----WTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
                W +S LLN+P+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ IN WA+HRDPNLWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERH--GDTSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 477
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

[Close]

BLAST of CmoCh20G009900 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 474.6 bits (1220), Expect = 9.7e-134
Identity = 248/498 (49.80%), Postives = 336/498 (67.47%), Query Frame = 0


Query: 4   LLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKYG 63
           L+ SI   +  L++    +   R+  LPP P +SLP++GHL LL  PIHRT  +LSQ   
Sbjct: 6   LIFSILFVVLSLIYLIGKL--KRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65

Query: 64  H--VFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYGE 123
           +  +FSLR G+RLV V SS S  +ECFTKND+VLANRP     K++ Y++T M  + YG+
Sbjct: 66  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125

Query: 124 HWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMF 183
           HWRNLRRI S+EIFS +RLN FL IR++EI+ L+ +L   +    F  V+++ ML DL F
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL-SRNFSQEFVKVDMKSMLSDLTF 185

Query: 184 NIVTRMVAGKRFY-------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKR 243
           N + RMVAGKR+Y           + R+++  ++A +GA N  D++P+ R +  S +E R
Sbjct: 186 NNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETR 245

Query: 244 VKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKG--------- 303
           VKKL    DE LQ LVDE R+  + GNTMI  LL+LQ ++P+Y++D+IIKG         
Sbjct: 246 VKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 305

Query: 304 ------LIQWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRL 363
                  ++W +S +LNHP+VL KAR EID  IG +RL++ESD+ +L Y+Q I+SETLRL
Sbjct: 306 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 365

Query: 364 HPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKN 423
           +PAAP+LLPH A E+CK+ GYD+PR TI+  N WA+HRDP LW+D  SFKPER     + 
Sbjct: 366 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPER----FEK 425

Query: 424 GAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPK 478
             E+ KL+PFGLGRRACPG G+A R+  LTL SLIQ  EWE++    VDMSEGKG+TMPK
Sbjct: 426 EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPK 485

[Close]

BLAST of CmoCh20G009900 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 473.4 bits (1217), Expect = 2.2e-133
Identity = 250/499 (50.10%), Postives = 337/499 (67.54%), Query Frame = 0


Query: 1   MDILLLSISLFIFPLLFAFNFVL--KPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNL 60
           MD+  + +  F+F L  +   +L    R+  LPP P + LP++GHLHLL  P+HRT  ++
Sbjct: 1   MDLTQILLLSFLF-LTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSI 60

Query: 61  SQKYGH--VFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSV 120
           S+  G+  +F LR G+RLV VISS S  +ECFTKND+VLANRP +   K++ YN T M  
Sbjct: 61  SKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIA 120

Query: 121 SPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPML 180
           + YG+HWRNLRRI+++EIFS++R++ F  IR++EI+ L+  L  DSL   F  VEL+ +L
Sbjct: 121 ASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHG-FVEVELKSLL 180

Query: 181 LDLMFNIVTRMVAGKRFY----ERHDK---FREMVTQIMAHSGATNPGDFIPLWRFIDPS 240
            +L FN +  MVAGKR+Y    E +D+    RE++ +IMA +G+ N  D++P   ++  +
Sbjct: 181 TNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--T 240

Query: 241 GFEKRVKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLI-- 300
            FE + K LG   D +LQ+LVDE R + + G T+I  LLS Q TEPEYY+D IIKG+I  
Sbjct: 241 NFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILA 300

Query: 301 -------------QWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 360
                        +W +S LLNHPE+LEKARAEID  IG +RLV ESD+ +L Y+Q I+S
Sbjct: 301 LVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVS 360

Query: 361 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 420
           ETLRL+PA PLLLPH + +ECK+ GYD+PR T++  N WAMHRDP LWE+   FKPER  
Sbjct: 361 ETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPER-- 420

Query: 421 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 474
              +   E+ KL+PFG+GRRACPG  + +R+  L L  LIQ FEWER+   LVDM+EG+G
Sbjct: 421 --FEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEG 480

[Close]

BLAST of CmoCh20G009900 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 471.9 bits (1213), Expect = 6.3e-133
Identity = 251/499 (50.30%), Postives = 335/499 (67.13%), Query Frame = 0


Query: 3   ILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKY 62
           ++L SI   IF L+ +F F LKP++  LPP P   LP++GHL LL  PIHRTL + S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----VFSLRFGSRLVVVISSPS-AVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSV 122
            H     V SLR GSRLV V+SS   A +ECF KND+VLANRP +   K++ YN+T M  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPML 182
           +PYG+HWRNLRR+ ++EIFST+RLN FL +R +E++ L+ +L       +  VVEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPML 187

Query: 183 LDLMFNIVTRMVAGKRFY-------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPS 242
           +DL FN + RM+ GKR+Y       E   + R++V  + A++ + N  D++P+ R    S
Sbjct: 188 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 247

Query: 243 GFEKRVKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLI-- 302
            +E RVKKLG+ +D+ LQ L+D+ R + + G TMI  LL LQ ++ EYY+DQIIKG+I  
Sbjct: 248 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307

Query: 303 -------------QWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 362
                        +W +S LLNHP+V+ KAR EID+ +G +RL+ E+DL  L Y++ I+ 
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 367

Query: 363 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 422
           ETLRLHPA PLL+PH A E+CKI  YD+PR T + +NAWA+HRDPN W+D  SFKPER  
Sbjct: 368 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER-- 427

Query: 423 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 474
              +   E+ KL+ FGLGRRACPG G+AQR+ GL L SLIQ FEWER+ N  VDM EG G
Sbjct: 428 --FEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487

[Close]

BLAST of CmoCh20G009900 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 469.9 bits (1208), Expect = 2.4e-132
Identity = 250/493 (50.71%), Postives = 335/493 (67.95%), Query Frame = 0


Query: 3   ILLLS-ISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQK 62
           IL+LS +SLF    L  F      R+L LPP P  SLP++GHLHLL  P+HRT  +LS+ 
Sbjct: 6   ILILSFLSLF---TLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKS 65

Query: 63  YGH--VFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPY 122
            G+  VF LR G+RLV VISS S  +ECFTKND+VLANRP    +K+L YN T +  + Y
Sbjct: 66  IGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASY 125

Query: 123 GEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDL 182
           G+HWRNLRRI+++EIFST+RLN FL IR++EI+ L+  L  DSL   F  VE++ +L +L
Sbjct: 126 GDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHG-FVEVEMKTLLTNL 185

Query: 183 MFNIVTRMVAGKRFY-ERHDK---FREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRV 242
             N   RM+AGKR++ E +D     + +V++ +  +GA NP D++ + R++  S +EKR+
Sbjct: 186 ASNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRI 245

Query: 243 KKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLI-------- 302
           K LG   D  LQ+LVDE R + + G TMI  LL+LQ+ +P+YY+D IIKG+I        
Sbjct: 246 KNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGT 305

Query: 303 -------QWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLH 362
                  +W +S LLNHPE+L+KAR EID  +G +RLV+ESD+ +LSY+Q I+ ETLR++
Sbjct: 306 DTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMY 365

Query: 363 PAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNG 422
           PA PLLLPH + E+CK+ GYD+P  T++  NAWAMHRDP +WED   FKPER     +  
Sbjct: 366 PAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER----FEKE 425

Query: 423 AESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKA 474
            E+ KLI FG+GRRACPG G+A R+    L SL+Q FEWER+    VDM+E KG T+PKA
Sbjct: 426 GEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKA 485

[Close]

BLAST of CmoCh20G009900 vs. TAIR 10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3 )

HSP 1 Score: 466.8 bits (1200), Expect = 2.0e-131
Identity = 242/501 (48.30%), Postives = 331/501 (66.07%), Query Frame = 0


Query: 3   ILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQKY 62
           +++L ++LF+  L +   F  K +R  LPP P +SLP+LGH +LL  P+HR    LS+ +
Sbjct: 5   VIVLPLALFL--LAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTH 64

Query: 63  GHVFSLRFGSRLVVVISSPSAVQECFT-KNDIVLANRPFLNSTKYLNYNHTVMSVSPYGE 122
           G +FSL+FGSR  VVISS S   +CFT +NDI+L+NRP   + KY+ YN+T +  +PYG+
Sbjct: 65  GPIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGD 124

Query: 123 HWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMF 182
           HWRNLRRI SLEI S+NRL  FL IR++EI  +L +L  D  ++    +ELEP+L DL F
Sbjct: 125 HWRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKE----IELEPLLSDLTF 184

Query: 183 NIVTRMVAGKRFY-------ERHDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKR 242
           N + RMV GKR+Y       E  + F+++V  I   SGA +PGD++P  +    S FEK+
Sbjct: 185 NNIVRMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKK 244

Query: 243 VKKLGKTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGL-------- 302
           VK L +  DE+LQ L++E +   D GNTM++ LLSLQ  EPEYY+D  IKGL        
Sbjct: 245 VKALAEAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLMLGMMIAG 304

Query: 303 -------IQWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRL 362
                  ++W +S LLNHPE LEKA+ EID  IG ERL++E D+ +L Y+Q I+SET RL
Sbjct: 305 TDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPYLQNIVSETFRL 364

Query: 363 HPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERH--ANGM 422
           +PAAPLL+P    E+ K+ GYDVPR T++ +NAWA+HRDP LW +   FKPER     G 
Sbjct: 365 YPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFNGGEGG 424

Query: 423 KNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITM 479
             G + +KL+PFG GRR+CPG G+ Q++  L L SLIQ F+W+++N   +DM+E  G+ M
Sbjct: 425 GRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTETPGMAM 484

The following BLAST results are available for this feature:

BLAST of CmoCh20G009900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.4e-145	52.40	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	8.9e-140	50.70	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ9	2.5e-134	50.90	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q6WNR0	1.6e-133	49.30	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]
Q9FG65	8.9e-132	50.30	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]

BLAST of CmoCh20G009900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTX9	5.5e-278	96.98	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=...	[more]
A0A6J1FXS5	2.3e-255	89.74	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=...	[more]
A0A6J1JDS1	4.6e-248	87.53	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 ...	[more]
A0A6J1G5P7	3.3e-206	72.82	cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1	[more]
A0A6J1L2W8	4.3e-206	72.41	cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1	[more]

BLAST of CmoCh20G009900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943757.1	1.1e-277	96.98	isoflavone 2'-hydroxylase-like [Cucurbita moschata]	[more]
XP_023512186.1	7.7e-266	91.94	isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022943410.1	4.7e-255	89.74	isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-...	[more]
XP_022985609.1	9.5e-248	87.53	isoflavone 2'-hydroxylase-like [Cucurbita maxima]	[more]
XP_022947035.1	6.9e-206	72.82	cytochrome P450 81F3 [Cucurbita moschata]	[more]

BLAST of CmoCh20G009900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	9.7e-134	49.80	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37320.1	2.2e-133	50.10	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT5G36220.1	6.3e-133	50.30	cytochrome p450 81d1	[more]
AT4G37330.1	2.4e-132	50.71	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37400.1	2.0e-131	48.30	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 296..309 score: 43.05 coord: 331..342 score: 39.84 coord: 407..416 score: 58.51
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 31..460 e-value: 2.7E-78 score: 263.8
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 59..78 score: 44.3 coord: 371..395 score: 30.4 coord: 83..104 score: 21.43 coord: 416..439 score: 32.89 coord: 287..313 score: 36.69 coord: 330..348 score: 36.76 coord: 406..416 score: 55.18
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 22..470 e-value: 7.2E-106 score: 356.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 31..470
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 8..476
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 8..476
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 409..418

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G009900.1	CmoCh20G009900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen