Homology
BLAST of CmoCh20G009860 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 500.4 bits (1287), Expect = 3.6e-140
Identity = 242/445 (54.38%), Postives = 330/445 (74.16%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
GHL+L+ + +H++L LS KYG VFSL+LG++LV+VVSSP+A +ECFT NDI+ ANRP
Sbjct: 44 GHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLF 103
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
+ K++ YN T S YG+HWR LRR++ +EIFSA LN FL R+DE+K++L L +
Sbjct: 104 ILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQS 163
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 474
S F +VEM S +SELS N+ MRMV GKR++G+ V D ++A+ FR ++ +V + G+ +
Sbjct: 164 SGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASN 223
Query: 475 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 534
F+P L WID +YEKK+ K+ Q D FLQ LI E R K TMIDHLLSLQ+SQ
Sbjct: 224 PGDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINK--NNVTMIDHLLSLQESQ 283
Query: 535 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 594
++ +DQ+IKG+IMV+LLAGTDT AVT+EWA+S LLN+P L+KAR E+++ +G RL+E
Sbjct: 284 PEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIE 343
Query: 595 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 654
E D+ KL+YL +ISET RL PAAP+LVPH +S++C + GY +P+ TI+L+NAWAIHRDP
Sbjct: 344 EQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDP 403
Query: 655 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 714
W++ F PER+ ++ KL+PFG+GRR+CPG G+A RVVGL+L +LIQCFEWKR
Sbjct: 404 EFWDEPTLFKPERHGGVELEP-SKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKR 463
Query: 715 VSSEVVDMTEGVGLTIPRAQPLEAM 740
+ +DM EG GLT+P+AQPLEA+
Sbjct: 464 IGEAKIDMAEGSGLTMPKAQPLEAL 484
BLAST of CmoCh20G009860 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 481.9 bits (1239), Expect = 1.3e-134
Identity = 242/447 (54.14%), Postives = 321/447 (71.81%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
G+L+ LK+P+H T LS KYG +FSL GS+LVVVVSS + +ECFT NDI+LANRP
Sbjct: 43 GNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHF 102
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
K++ YNNT + S YGDHWR LRRI +IEI S+ RLN FLE R+DEI R++ KL +
Sbjct: 103 LTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQK 162
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGE--GVLDLEQARKFRDIVKQVMQKCGS 474
S + F VE+ M SE++ N +MRMV GKR+YG V D+E+AR FR I+K+V+ G+
Sbjct: 163 SYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGA 222
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
+ F+ L W D EK++ K+ +R D FLQGLIDEHR K NTMIDHLL+ QQ
Sbjct: 223 NNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNS-NTMIDHLLTQQQ 282
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
SQ ++ +DQ+IKGL++V+LLAGTDT +VTIEWA+S+LLN+P ++KKA+ ELD+ IG +R
Sbjct: 283 SQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQ 342
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
V+E D+SKL YLQ ++ ETLRL+ AAPLLVPH +SE+ + GY IP++TI+++NAW IHR
Sbjct: 343 VDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHR 402
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DPN+W D F PER++ ++ KL+ FGLGRRACPG ++ R GL+L LIQCFEW
Sbjct: 403 DPNLWSDPTCFKPERFE--KEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEW 462
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
KR+ E +DM E G+T + L AM
Sbjct: 463 KRIGEEKIDMVEAKGITAGKKTSLNAM 486
BLAST of CmoCh20G009860 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 480.3 bits (1235), Expect = 3.8e-134
Identity = 240/451 (53.22%), Postives = 331/451 (73.39%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLS-----NKYGHVFSLRLGSQLVVVVSSPS-AVKECFTMNDIIL 354
GHL LLK P+H+TL+ S N G V SLRLGS+LV VVSS A +ECF ND++L
Sbjct: 45 GHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVL 104
Query: 355 ANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVL 414
ANRP I KH+ YNNT A+ YGDHWR LRR+ TIEIFS RLN FL R DE++R++
Sbjct: 105 ANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLI 164
Query: 415 GKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQ 474
+L R++ VE+ M+ +L+ N +MRM+ GKR+YGE D E+A++ R +V V
Sbjct: 165 SRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGA 224
Query: 475 KCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLL 534
S ++ ++P+L + YE ++ KLG+ D+FLQGLID+ R Q+E G TMIDHLL
Sbjct: 225 NTSSGNAVDYVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLL 284
Query: 535 SLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIG 594
LQ+S +++ +DQ+IKG+I+++++AGT+T AVT+EWALS+LLN+P+V+ KAR E+D+ +G
Sbjct: 285 VLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG 344
Query: 595 QERLVEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAW 654
+RL+EE+D+S+L YL+ ++ ETLRL+PA PLLVPH ASE+C I Y +PR T +L+NAW
Sbjct: 345 LDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAW 404
Query: 655 AIHRDPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQ 714
AIHRDPN W+D ++F PER++ ++ KL+ FGLGRRACPG G+A R+VGL+L SLIQ
Sbjct: 405 AIHRDPNTWDDPDSFKPERFE--KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQ 464
Query: 715 CFEWKRVSSEVVDMTEGVGLTIPRAQPLEAM 740
CFEW+RV + VDM EGVG T+P+A PL+A+
Sbjct: 465 CFEWERVGNVEVDMKEGVGNTVPKAIPLKAI 490
BLAST of CmoCh20G009860 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 479.6 bits (1233), Expect = 6.5e-134
Identity = 232/447 (51.90%), Postives = 333/447 (74.50%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
G+L+ LKRP+H+T + LS KYGHVFSL GS+LVVVVSS S ++CFT ND++LANRP
Sbjct: 43 GNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRF 102
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
K++ YN T ++SYG+HWR LRRI+ +++ S R+N F R+DE +R++ +L
Sbjct: 103 LSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADD 162
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGE--GVLDLEQARKFRDIVKQVMQKCGS 474
SS +FA +E+SS + +++ N +MRM+ GKR+YGE DL++A +FRD+V +++Q G+
Sbjct: 163 SSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGA 222
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
+ F+PLL ++D + EK++ + + D FL+GLI+EHR +KE NTMIDHLL+LQ
Sbjct: 223 NNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERA-NTMIDHLLNLQD 282
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
SQ ++ +DQ+IKGL + +LLAGTD+ AVT+EW++S+LLN+P VLKK + ELD+ +GQ+RL
Sbjct: 283 SQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRL 342
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
V+ESD+ KL+YL+ VI+ETLRL APLL+PH S+ C I GYK+P+DTI+LINAWAIHR
Sbjct: 343 VDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHR 402
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DP +W +A TF PER++ +L KLI FG+GRRACPG G+A R + ++LA LIQCF+W
Sbjct: 403 DPELWTEATTFKPERFE--KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW 462
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
K ++ + +D+ E G T+ + PL+AM
Sbjct: 463 KLINGDKIDLAERDGFTLTKLVPLKAM 486
BLAST of CmoCh20G009860 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 470.7 bits (1210), Expect = 3.0e-131
Identity = 238/452 (52.65%), Postives = 328/452 (72.57%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
G+L+ LK P+H+T LS YG +FSL GS+LVVVVSSPS ECFT NDIILANRP
Sbjct: 41 GNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRF 100
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCR- 414
K++ YN T ++SYGDHWR LRRI+TI++ S +RLN FL R+DE R++ KL +
Sbjct: 101 LTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKD 160
Query: 415 ISSD--SFARVEMSSMISELSSNIVMRMVMGKRFYGE--GVLDLEQARKFRDIVKQVMQK 474
+ S+ F +VE+ ++E++ N +MRM+ GKR+YG+ V D+E+A++FR+I+ ++M
Sbjct: 161 VVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSL 220
Query: 475 CGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHR--NQKEEGRNTMIDHL 534
G+ + F+PLL +D+ + EK+ ++ +R++ FL+GLI+EHR N +G TMIDHL
Sbjct: 221 LGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDG-GTMIDHL 280
Query: 535 LSLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSII 594
L L +SQ ++ SD +IKGLI +LLAGTDT AVTIEW +S LLN+P VLKKA+ ELD+ I
Sbjct: 281 LKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQI 340
Query: 595 GQERLVEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINA 654
G+ +LV+E D+SKL YLQ +ISETLRL+P APLL+PHY+SE+C I + +P+DTIIL N
Sbjct: 341 GKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNV 400
Query: 655 WAIHRDPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLI 714
W IHRDP W DA +F PER++ +++ K++ FGLGRRACPG+ +A R VG ++ LI
Sbjct: 401 WGIHRDPKHWNDALSFKPERFE--KEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLI 460
Query: 715 QCFEWKRVSSEVVDMTEGVGLTIPRAQPLEAM 740
QCFEW+R S E +DM EG G+T+P PL AM
Sbjct: 461 QCFEWERESEEKLDMMEGKGITMPMKIPLRAM 489
BLAST of CmoCh20G009860 vs. ExPASy TrEMBL
Match:
A0A6J1FUI0 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=1)
HSP 1 Score: 882.5 bits (2279), Expect = 1.2e-252
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS
Sbjct: 41 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 100
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI
Sbjct: 101 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 160
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 474
SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD
Sbjct: 161 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 220
Query: 475 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 534
SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ
Sbjct: 221 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 280
Query: 535 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 594
LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE
Sbjct: 281 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 340
Query: 595 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 654
ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP
Sbjct: 341 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 400
Query: 655 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 714
NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR
Sbjct: 401 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 460
Query: 715 VSSEVVDMTEGVGLTIPRAQPLEAM 740
VSSEVVDMTEGVGLTIPRAQPLEAM
Sbjct: 461 VSSEVVDMTEGVGLTIPRAQPLEAM 485
BLAST of CmoCh20G009860 vs. ExPASy TrEMBL
Match:
A5AJI9 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_015004 PE=3 SV=1)
HSP 1 Score: 831.6 bits (2147), Expect = 2.5e-237
Identity = 407/799 (50.94%), Postives = 562/799 (70.34%), Query Frame = 0
Query: 1 MFLDLMYNVIMRMVAGNKPYEH--DKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDP 60
+F +L +N+I RMVAG + Y D ++ FRE++ K + +NPGDF+PIL W+D
Sbjct: 175 LFSELTFNIITRMVAGKRYYGEGSDFEEAKHFREIIRKSFLLSAASNPGDFLPILRWMDY 234
Query: 61 TGLEKKIMKLGRKMDELLQELVDGIRYQKGTG----NTMIDRLLHLQKTDPQNHTDQIIK 120
G EKK+ K R++D +LQ L+D R G NTMID LL LQK++P+ +TDQIIK
Sbjct: 235 GGYEKKMAKNSRELDVILQGLIDEHRSNSKKGLMGNNTMIDHLLSLQKSEPEYYTDQIIK 294
Query: 121 GLIQIILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYL 180
G+ ++ AG DT AVT+EWA+S LLN+P+VL+KAK E+D+ +GQE L+ EADL L+YL
Sbjct: 295 GVTMNLVFAGTDTAAVTMEWAMSLLLNHPDVLKKAKVELDTXVGQERLLEEADLPKLHYL 354
Query: 181 QGIISETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFK 240
Q IISET RL P APL +PH +S + ++GG+D+PRD ++L+N+W +HRDP LW+D T FK
Sbjct: 355 QNIISETFRLCPPAPLWLPHMSSANCQLGGFDIPRDXMLLVNSWTLHRDPKLWDDPTSFK 414
Query: 241 PERHANPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE 300
PER ++YK LPFG GRRACPG G+A ++ LTLG LIQC+EWER VDM E
Sbjct: 415 PERFEGGERGETYKLLPFGTGRRACPGSGLANKVVGLTLGSLIQCYEWERISEKKVDMME 474
Query: 301 ---------------------------------------------------GHLYLLKRP 360
GHL+LLK P
Sbjct: 475 GKGLTMPKMEPLEAMCSAYEILKXVLQDEETMRNKGKRLNLPPSPPGFPIXGHLHLLKGP 534
Query: 361 VHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYN 420
+H+TL +LS ++G + SLR GS+ V+VVSSPSAV+ECFT ND+I ANRP + K++ Y+
Sbjct: 535 LHRTLHRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFTKNDVIFANRPKFVMGKYIGYD 594
Query: 421 NTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVE 480
T+ S + YGDHWR LRR+S +EIF+++RLN+FL R+DEIK++L +L R S ++FA+VE
Sbjct: 595 YTVVSLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKQLLLRLSRNSVENFAKVE 654
Query: 481 MSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLN 540
+ SM SEL NI MRMV GKRFYG+ + D+E+AR+FR+I K++++ G+ + F P+L
Sbjct: 655 LKSMFSELLLNITMRMVAGKRFYGDNMKDVEEAREFREISKEILEFXGTSNPGDFXPILQ 714
Query: 541 WIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKE---EGRNTMIDHLLSLQQSQLDFMSD 600
WID Y K+ ++LG++ D FLQGL+DE R+ K E RNTMIDHLLSLQ+S+ ++ +D
Sbjct: 715 WIDYQGYNKRALRLGKKMDVFLQGLLDECRSNKRSDLENRNTMIDHLLSLQESEPEYYTD 774
Query: 601 QVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSK 660
++IKGLI+ + + G DT AVTIEWA+S LLN+P VLKKAR ELD+ IG + L++E+D+ K
Sbjct: 775 EIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETDLPK 834
Query: 661 LSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDA 720
L YLQ +ISE+LRL P+ PLLVPH+++E+C + G+ +P T++L+NAWA+HRDP +W D
Sbjct: 835 LQYLQSIISESLRLFPSTPLLVPHFSTEDCKLGGFDVPGGTMLLVNAWALHRDPKLWNDP 894
Query: 721 NTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKRVSSEVV 740
+F PER++ T + YKL+PFG+GRRACPG+G+A+RV+GL+L SLIQCF+WKRV + +
Sbjct: 895 TSFKPERFE-TGESETYKLLPFGVGRRACPGIGLANRVMGLTLGSLIQCFDWKRVDEKEI 954
BLAST of CmoCh20G009860 vs. ExPASy TrEMBL
Match:
A0A7J6G3K7 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_006841 PE=3 SV=1)
HSP 1 Score: 830.9 bits (2145), Expect = 4.3e-237
Identity = 433/785 (55.16%), Postives = 560/785 (71.34%), Query Frame = 0
Query: 4 DLMYNVIMRMVAGNKPYEHDKGRSRE---FRELVTKIMAVGGTTNPGDFIPI-LNWIDPT 63
++ +N+IMRMVAG + Y D E F + + ++ G NP DF+P+ L W+
Sbjct: 64 NMTFNIIMRMVAGKRYYGDDVANKEEANMFMRIFDEAVSSSGVANPADFLPVGLKWL-AM 123
Query: 64 GLEKKIMKLGRKMDELLQELVDGIRYQKGTGNTMIDRLLHLQKTDPQNHTDQIIKGLIQI 123
E K+ + +KMD LQ LVD R K + TMID LL LQ++ P+ ++D+IIKG I +
Sbjct: 124 SYELKMKRASKKMDAYLQRLVDERRSMK-SDITMIDHLLALQESHPEYYSDEIIKGFILV 183
Query: 124 ILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYLQGIIS 183
+L+AG DT++VTLEWALS+LLN+PEVLEKAKAEID IG++ L+ E D+S L YL IIS
Sbjct: 184 LLLAGTDTSSVTLEWALSNLLNHPEVLEKAKAEIDEKIGEK-LMEEEDISKLPYLNNIIS 243
Query: 184 ETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFKPERHA 243
ETLRL P AP+L+PH +S+D I GYD+PRDT+V++N WAIHRDP LW+D FKPER
Sbjct: 244 ETLRLFPPAPMLLPHYSSEDCTIEGYDIPRDTMVMVNVWAIHRDPKLWDDPLSFKPERFE 303
Query: 244 NPIGIDSYKFL-PFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE---- 303
I+ K+L PFGLGRRACPG G+AQR+ LTLG LIQCFEW+R L
Sbjct: 304 R---INDEKYLMPFGLGRRACPGHGLAQRVLGLTLGSLIQCFEWKRIDPFLYGSLSFITI 363
Query: 304 ----------------------------GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQ 363
GHL+LLK VH+ LS KYG VF+L GS+
Sbjct: 364 IILIFFIKTKNKKHYKNLPPSPPSYPIIGHLHLLKPRVHRIFHSLSAKYGDVFTLWFGSR 423
Query: 364 LVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIE 423
VVVVSSPSAV+ECFT NDI+LANRP + KH++YN T A+ YGDHWR LRRI +E
Sbjct: 424 RVVVVSSPSAVEECFTKNDIVLANRPPLLMGKHISYNYTTIVAAPYGDHWRNLRRIGAVE 483
Query: 424 IFSASRLNMFLETRKDEIKRVLGKLCR-ISSDSFARVEMSSMISELSSNIVMRMVMGKRF 483
IFS +RLN+ L RKDEI R+L K+ R ISS++ VEM SM++EL+ NIVMRM+ GKR+
Sbjct: 484 IFSTARLNLLLNIRKDEIDRLLRKINRSISSNTDRAVEMKSMLTELTFNIVMRMMAGKRY 543
Query: 484 YGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFL 543
YG+ V D E+ARKFR+I+K V + G+ + F+P+LNW+ YEK+I KL ++ D FL
Sbjct: 544 YGDDVTDDEEARKFREIMKAVFENGGAGNQADFLPILNWVP-NSYEKRIEKLAKKTDSFL 603
Query: 544 QGLIDEHRNQKEE-------GRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAGTDTI 603
QGLIDEHRN+K++ RNTMIDHLL+LQQSQ ++ +DQ+IKG ++++LLAGTDT
Sbjct: 604 QGLIDEHRNRKDDDDQNSNLDRNTMIDHLLTLQQSQPEYYTDQIIKGFVLILLLAGTDTS 663
Query: 604 AVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRLNPAA 663
AVT+EW +S+LLN+P+VL+K + ELDS IGQ++LV+ESD+SKL YLQ VISETLRL PAA
Sbjct: 664 AVTLEWTMSNLLNHPHVLQKLKDELDSQIGQQQLVDESDLSKLPYLQNVISETLRLYPAA 723
Query: 664 PLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPERY----KITMVD 723
PLLVPHY+S +C I+G+ +PRDTI+L+NAWAIHRDP +WEDA F PER+ K +
Sbjct: 724 PLLVPHYSSNDCTISGFDVPRDTIVLVNAWAIHRDPKLWEDAEIFKPERFENIGKGSENG 783
Query: 724 DLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKRVSSEVVDMTEGVGLTIPRAQ 740
+ YKL+PFGLGRR+CPG+G+A RVVGL+L SLIQCFEW+RVS E +DM+E GLT+P+
Sbjct: 784 EGYKLMPFGLGRRSCPGIGLAQRVVGLTLGSLIQCFEWERVSEEKIDMSENKGLTMPKLV 841
BLAST of CmoCh20G009860 vs. ExPASy TrEMBL
Match:
A0A371E3R5 (Cytochrome P450 81E8 (Fragment) OS=Mucuna pruriens OX=157652 GN=CYP81E8 PE=3 SV=1)
HSP 1 Score: 817.8 bits (2111), Expect = 3.7e-233
Identity = 412/808 (50.99%), Postives = 551/808 (68.19%), Query Frame = 0
Query: 1 MFLDLMYNVIMRMVAGNKPY--EH---DKGRSREFRELVTKIMAVGGTTNPGDFIPILNW 60
MF +L +N+IMRMV G + Y EH D +R+FRE+V +I G +N GDF+P+ W
Sbjct: 173 MFAELTFNIIMRMVCGKRYYGEEHDGTDPEEARKFREIVNEISQFGLGSNLGDFVPVFRW 232
Query: 61 IDPTGLEKKIMKLGRKMDELLQELVDGIRYQKGTGNTMIDRLLHLQKTDPQNHTDQIIKG 120
D +G +K+ K+G K+D Q L+D R +K + NTMI LL Q++ P+ +TDQIIKG
Sbjct: 233 FDFSG-PRKLRKVGEKLDSFFQGLIDEHRNKKESSNTMIGHLLSSQESQPEYYTDQIIKG 292
Query: 121 LIQIILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYLQ 180
LI + +AG +T+AVTLEWA+S+LLN+PE+LEKA+ E+D+ +G+E LV EAD+S L YLQ
Sbjct: 293 LIMALYVAGTETSAVTLEWAMSNLLNHPEILEKARVELDTKVGRERLVEEADVSKLEYLQ 352
Query: 181 GIISETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFKP 240
IISETLRL P P+L+PH +S+D +GGYDVPR+T++ +NAWAIHRDP LW D T FKP
Sbjct: 353 NIISETLRLHPPLPMLLPHFSSQDCTVGGYDVPRNTMLFVNAWAIHRDPELWTDPTSFKP 412
Query: 241 ERHANPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE- 300
ER + ++++K +PFGLGRRACPG G+AQR TL LIQCFEW+R G VD+++
Sbjct: 413 ERFQSG-AVEAHKLIPFGLGRRACPGAGLAQRTVGWTLASLIQCFEWKRIGEEEVDLTDG 472
Query: 301 ------------------------------------------------------------ 360
Sbjct: 473 RGTVVPKLIPLEAQCRARPIICPHNLMSIILLFLLFLMTLNLLRLRKRHKNPPPSPPSLP 532
Query: 361 --GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 420
G+L+ LK+P+H+TL LSNKYG VFSLR GSQ VVVVSS SA +ECFT NDIILANR
Sbjct: 533 IIGNLHQLKQPLHRTLHALSNKYGPVFSLRFGSQAVVVVSSASAAEECFTKNDIILANRF 592
Query: 421 SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 480
S K+L +N+TI A+SYGDHWR LRRIS++EI S RLN FLE RKDE +++L KL
Sbjct: 593 QSIKSKYLGFNHTILLAASYGDHWRNLRRISSLEILSTHRLNSFLEIRKDETQKLLRKLA 652
Query: 481 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGV--LDLEQARKFRDIVKQVMQKC 540
R S + FAR+E S+ ++L+ N +MRMV GKR+YGE + E+A KFRDI+ ++ Q
Sbjct: 653 RASKEDFARLEFRSLFADLTFNTIMRMVCGKRYYGEEFDGTNAEEANKFRDIMNEMSQFG 712
Query: 541 GSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSL 600
F+PL D + K+ K G++ D QGL+DEHRN K E NTMI+HLLSL
Sbjct: 713 LGSHLGDFVPLFRLFDFSGSHNKLRKTGEKMDALFQGLVDEHRN-KNENSNTMINHLLSL 772
Query: 601 QQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQE 660
Q SQ ++ +D++IKGLIMV+++AGT+T A+ +EWA+S+LLN+P VL+KAR+ELD+++GQE
Sbjct: 773 QDSQPEYYTDEIIKGLIMVLIVAGTETSAIALEWAMSNLLNHPQVLEKARVELDALVGQE 832
Query: 661 RLVEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAI 720
RL+EE++++KL YLQ +ISETLRL+P AP+L+PH++SE+C + GY +PR T++ +NAW I
Sbjct: 833 RLIEEAEVTKLEYLQNIISETLRLHPPAPMLLPHFSSEDCTVGGYDVPRSTMLFVNAWTI 892
Query: 721 HRDPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCF 739
HRDP +W D +F PER++ V+ +IPFGLGRRACPG +A R VG +LASLIQCF
Sbjct: 893 HRDPELWTDPTSFKPERFQNGTVEAQKLIIPFGLGRRACPGASLAQRTVGWTLASLIQCF 952
BLAST of CmoCh20G009860 vs. ExPASy TrEMBL
Match:
F6HSX8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0129g00860 PE=3 SV=1)
HSP 1 Score: 812.0 bits (2096), Expect = 2.1e-231
Identity = 407/854 (47.66%), Postives = 563/854 (65.93%), Query Frame = 0
Query: 1 MFLDLMYNVIMRMVAGNKPYEH--DKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDP 60
+F +L +N+I RMVAG + Y D ++ FRE++ K + +NPGDF+PIL W+D
Sbjct: 175 LFSELTFNIITRMVAGKRYYGEGSDFEEAKHFREIIRKSFLLSAASNPGDFLPILRWMDY 234
Query: 61 TGLEKKIMKLGRKMDELLQELVDGIRYQKGTG----NTMIDRLLHLQKTDPQNHTDQIIK 120
G EKK+ K R++D +LQ L+D R G NTMID LL LQK++P+ +TDQIIK
Sbjct: 235 GGYEKKMAKNSRELDVILQGLIDEHRSNSKKGLMGNNTMIDHLLSLQKSEPEYYTDQIIK 294
Query: 121 GLIQIILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYL 180
G+ ++ AG DT AVT+EWA+S LLN+P+VL+KAK E+D+ +GQE L+ EADL L+YL
Sbjct: 295 GVTMNLVFAGTDTAAVTMEWAMSLLLNHPDVLKKAKVELDTCVGQERLLEEADLPKLHYL 354
Query: 181 QGIISETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFK 240
Q IISET RL P APL +PH +S + ++GG+D+PRD ++L+N+W +HRDP LW+D T FK
Sbjct: 355 QNIISETFRLCPPAPLWLPHMSSANCQLGGFDIPRDAMLLVNSWTLHRDPKLWDDPTSFK 414
Query: 241 PERHANPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE 300
PER ++YK LPFG GRRACPG G+A ++ LTLG LIQC+EWER VDM E
Sbjct: 415 PERFEGGERGETYKLLPFGTGRRACPGSGLANKVVGLTLGSLIQCYEWERISEKKVDMME 474
Query: 301 ------------------------------------------------------------ 360
Sbjct: 475 GKGLTMPKMEPLEAMCSAYEILKNVLQDEETMQGELQHQYTTWSARYSDLINRSRSPFQS 534
Query: 361 ----------------------------------------------GHLYLLKRPVHQTL 420
GHL+LLK P+H+TL
Sbjct: 535 NQSMEARWLYSSLSFLFFALAVKFLLQRNKGKRLNLPPSPPGFPIFGHLHLLKGPLHRTL 594
Query: 421 QKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFS 480
+LS ++G + SLR GS+ V+VVSSPSAV+ECFT ND+I ANRP + K++ Y+ T+ S
Sbjct: 595 HRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFTKNDVIFANRPKFVMGKYIGYDYTVVS 654
Query: 481 ASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMI 540
+ YGDHWR LRR+S +EIF+++RLN+FL R+DEIK++L +L R S ++FA+VE+ SM
Sbjct: 655 LAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKQLLLRLSRNSVENFAKVELKSMF 714
Query: 541 SELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDIT 600
SEL NI MRMV GKRFYG+ + D+E+AR+FR+I K++++ G+ + F+P+L WID
Sbjct: 715 SELLLNITMRMVAGKRFYGDNMKDVEEAREFREISKEILEFSGTSNPGDFLPILQWIDYQ 774
Query: 601 HYEKKIIKLGQRNDEFLQGLIDEHRNQKE---EGRNTMIDHLLSLQQSQLDFMSDQVIKG 660
Y K+ ++LG++ D FLQGL+DE R+ K E RNTMIDHLLSLQ+S+ ++ +D++IKG
Sbjct: 775 GYNKRALRLGKKMDVFLQGLLDECRSNKRSDLENRNTMIDHLLSLQESEPEYYTDEIIKG 834
Query: 661 LIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQ 720
LI+ + + G DT AVTIEWA+S LLN+P VLKKAR ELD+ IG + L++E+D+ KL YLQ
Sbjct: 835 LIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETDLPKLQYLQ 894
Query: 721 GVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNP 740
+ISE+LRL P+ PLLVPH+++E+C + G+ +P T++L+NAWA+HRDP +W D +F P
Sbjct: 895 SIISESLRLFPSTPLLVPHFSTEDCKLRGFDVPGGTMLLVNAWALHRDPKLWNDPTSFKP 954
BLAST of CmoCh20G009860 vs. NCBI nr
Match:
KAG7034318.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 891.7 bits (2303), Expect = 4.2e-255
Identity = 448/783 (57.22%), Postives = 570/783 (72.80%), Query Frame = 0
Query: 4 DLMYNVIMRMVAGNKPYEH---DKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDPTG 63
+L +N+ MRM AG + + D+ +R+FREL+ +I+++GG +NPGDFIP+LNWI P
Sbjct: 183 ELTFNISMRMAAGKRYFGDDVADEDEARQFRELIKQIVSLGGVSNPGDFIPMLNWI-PNV 242
Query: 64 LEKKIMKLGRKMDELLQELVDGIRYQK-GTGNTMIDRLLHLQKTDPQNHTDQIIKGLIQI 123
E+K+ +L ++ D LQ L+D R K NTMID LL LQ+++P+ + DQIIKG+I +
Sbjct: 243 FERKVSRLAKRTDAFLQGLIDEHRSNKEEERNTMIDHLLSLQQSEPELYGDQIIKGIILV 302
Query: 124 ILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYLQGIIS 183
+L+AG DT+AVT+EWALSHLLNNPE+L+KA+ E+D+ IGQE LV E+D+ L YLQGIIS
Sbjct: 303 LLLAGTDTSAVTMEWALSHLLNNPEILKKAREELDAQIGQERLVEESDVPKLPYLQGIIS 362
Query: 184 ETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFKPERHA 243
ETLRLTPAAPLLVPH S D I Y VPRDTIVL+NAWAIHRDPS WED TRF PERH
Sbjct: 363 ETLRLTPAAPLLVPHLTSDDCTISNYKVPRDTIVLVNAWAIHRDPSQWEDPTRFNPERHR 422
Query: 244 NPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE----- 303
G ++KFLPFG+GRR+CPG G+AQR+ L L LIQC+EWER G VDM+E
Sbjct: 423 KSEGDHAHKFLPFGIGRRSCPGSGMAQRVVGLALAALIQCYEWERIGEEKVDMAEGRGIT 482
Query: 304 --------------------------------------GHLYLLKRPVHQTLQKLSNKYG 363
GHL+ LK PVH+T Q LS KYG
Sbjct: 483 MPKLTLAHSPSRRAQIWRFRYLRRRRNLPPTPPSLPIIGHLHYLKVPVHRTFQNLSAKYG 542
Query: 364 HVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGDHW 423
VFSL LGS+LVVVVSS AV+ECFT ND++LANRP + K + YN T S YGDHW
Sbjct: 543 PVFSLWLGSRLVVVVSSSEAVEECFTKNDVVLANRPKFLVGKLVAYNCTTVVGSQYGDHW 602
Query: 424 RELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSNIV 483
R LRRI +EIFSASRLN F R +E++R+L KL R S F++V+M S+ISEL+ NI
Sbjct: 603 RNLRRIGAMEIFSASRLNQFAGIRMEELQRLLRKLSRNSFHGFSKVDMQSLISELTFNIS 662
Query: 484 MRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKIIK 543
MR+ GKR++G V D E+AR+FR+++KQ G FIP+LNWI +E+K++K
Sbjct: 663 MRIAAGKRYFGSDVTDEEEARQFREMIKQAASVAGVSYPGDFIPILNWIS-KAFERKLLK 722
Query: 544 LGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAGTD 603
LG+R D FLQGLID+HR+ KEEGR+TMID+LLSLQ+S + +DQVIKG++ V+LLAGT+
Sbjct: 723 LGKRMDTFLQGLIDQHRSNKEEGRDTMIDNLLSLQESDPTYYNDQVIKGMVQVLLLAGTE 782
Query: 604 TIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRLNP 663
T+AVT+EWAL+HLLNNP +LKKAR E+D+ IG+ERLV+ESD SKL Y+Q VI ETLRLNP
Sbjct: 783 TLAVTMEWALAHLLNNPEILKKAREEVDTQIGEERLVDESDASKLPYVQAVILETLRLNP 842
Query: 664 AAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPERYKITMVDDL 723
A+PLLVPH S++C I+ YKIPRDTI+L+NAWA+HRDPN WED FNP+R+ + D L
Sbjct: 843 ASPLLVPHLTSDDCTISNYKIPRDTIVLVNAWALHRDPNQWEDPTRFNPDRHLESEGDML 902
Query: 724 YKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKRVSSEVVDMTEGVGLTIPRAQPL 740
YKL+PFG+GRR+CPG MA R +GL+L +LIQC+EW+RV E+VDM EG G+T+P+A PL
Sbjct: 903 YKLVPFGVGRRSCPGAVMAQRTMGLTLGALIQCYEWERVREEIVDMREGRGVTMPKAVPL 962
BLAST of CmoCh20G009860 vs. NCBI nr
Match:
XP_022944471.1 (cytochrome P450 81D1-like [Cucurbita moschata])
HSP 1 Score: 882.5 bits (2279), Expect = 2.6e-252
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS
Sbjct: 41 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 100
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI
Sbjct: 101 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 160
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 474
SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD
Sbjct: 161 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 220
Query: 475 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 534
SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ
Sbjct: 221 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 280
Query: 535 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 594
LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE
Sbjct: 281 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 340
Query: 595 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 654
ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP
Sbjct: 341 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 400
Query: 655 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 714
NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR
Sbjct: 401 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 460
Query: 715 VSSEVVDMTEGVGLTIPRAQPLEAM 740
VSSEVVDMTEGVGLTIPRAQPLEAM
Sbjct: 461 VSSEVVDMTEGVGLTIPRAQPLEAM 485
BLAST of CmoCh20G009860 vs. NCBI nr
Match:
XP_023513339.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 855.1 bits (2208), Expect = 4.4e-244
Identity = 431/445 (96.85%), Postives = 437/445 (98.20%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSS 354
GHLYLLKRPVHQTLQKLSNKYGHVF+LRLGSQLVVVVSSPS VKECFTMNDIILANRPSS
Sbjct: 41 GHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRPSS 100
Query: 355 NIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRI 414
NIIKHLTYNNTIFSAS YGDHWRELRRISTIEIFSA+RLNMFLETRKDEIK +LGKLC I
Sbjct: 101 NIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLCCI 160
Query: 415 SSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFD 474
SSDSF RVEMSSMISELSSNIVMRMVMGKRFYGEGV DLEQARKFRDIVKQVMQKCGSFD
Sbjct: 161 SSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQVMQKCGSFD 220
Query: 475 SRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 534
SRSFIPLLNWIDITHYEK+IIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ
Sbjct: 221 SRSFIPLLNWIDITHYEKRIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQSQ 280
Query: 535 LDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 594
LDFMSDQVIKGLI VILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE
Sbjct: 281 LDFMSDQVIKGLITVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVE 340
Query: 595 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDP 654
ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENC IAGY+IPRDTIILINAWAIHRDP
Sbjct: 341 ESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCSIAGYEIPRDTIILINAWAIHRDP 400
Query: 655 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 714
NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR
Sbjct: 401 NIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKR 460
Query: 715 VSSEVVDMTEGVGLTIPRAQPLEAM 740
VSSE+VDMTEGVGLTIPRAQPLEAM
Sbjct: 461 VSSELVDMTEGVGLTIPRAQPLEAM 485
BLAST of CmoCh20G009860 vs. NCBI nr
Match:
XP_004141998.3 (uncharacterized protein LOC101215922 [Cucumis sativus])
HSP 1 Score: 842.0 bits (2174), Expect = 3.8e-240
Identity = 438/834 (52.52%), Postives = 568/834 (68.11%), Query Frame = 0
Query: 4 DLMYNVIMRMVAGNKPY---EHDKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDPTG 63
+L +N+ MRM AG + + D +R+ REL+ +I+++GG +NPGDFIP++NW+ P G
Sbjct: 175 ELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWV-PNG 234
Query: 64 LEKKIMKLGRKMDELLQELVDGIRYQK-GTGNTMIDRLLHLQKTDPQNHTDQIIKGLIQI 123
++K+ +LG++MD LQ L+D R K NTMID LL LQ+T+P+ + D+IIKG++ +
Sbjct: 235 YKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDEIIKGIVLV 294
Query: 124 ILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYLQGIIS 183
+L+AG DT+AVT+EWAL+HLLNNPEVL+KA+ E+D+ IG++ L E D+S L YLQGII
Sbjct: 295 LLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIGEKQLAEELDVSKLPYLQGIIY 354
Query: 184 ETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFKPERHA 243
ETLRL PAAP+LVPH S + I Y++PRDTIVL+NAWAIHRDPS WE+ T FKPERH
Sbjct: 355 ETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAWAIHRDPSQWEEPTLFKPERHQ 414
Query: 244 NPIGIDSY---KFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE-- 303
N D + K +PFG+GRRACPG +AQR+ LTL LIQC+EWER G VDM+E
Sbjct: 415 NLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLATLIQCYEWERIGEEKVDMTEGR 474
Query: 304 ------------------------------------------------------------ 363
Sbjct: 475 GVTMPKVVPLEAMCKARPIVHNIFNPPLHLHSPTPNLNITFFNKNAMQIYSLLYLFLSLI 534
Query: 364 -------------------------GHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVV 423
GHL+ LK P HQTL KLS +YG V SL LGS+LVV
Sbjct: 535 SLLFAFNLRTRRTNLPPSPPSLPIIGHLHYLKIPHHQTLHKLSAQYGPVISLWLGSRLVV 594
Query: 424 VVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFS 483
VVSS AV+ECFT NDI+LANRP + KH++YN T +S YGDHWR LRRI IEIFS
Sbjct: 595 VVSSLPAVEECFTKNDIVLANRPRLLVGKHISYNYTTMVSSPYGDHWRNLRRIGAIEIFS 654
Query: 484 ASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEG 543
ASRLN F +RKDE++R+L KL R S F++VEM S ISE + NI MRM GKR++GE
Sbjct: 655 ASRLNKFAGSRKDEVERLLKKLSRNSMYGFSKVEMQSAISERTFNISMRMAAGKRYFGEE 714
Query: 544 VLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLI 603
V D+E+AR+ R+++KQ++ G FIP++NWI +++K+ ++ +R D FLQGLI
Sbjct: 715 VRDVEEARQSRELIKQIVSMGGVSYPGDFIPMMNWIP-NGFKRKVWRVAKRMDAFLQGLI 774
Query: 604 DEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHL 663
DEHR+ KEE RNT+I HLLSLQ+ + ++ D++IKG+++ +LLAG DT AVTIEW L+HL
Sbjct: 775 DEHRSNKEEERNTVIGHLLSLQEMEPEYYGDEIIKGIVL-LLLAGVDTSAVTIEWILAHL 834
Query: 664 LNNPNVLKKARIELDSIIGQERLVEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASEN 723
LNNP+VLKKAR E+D+ IG++RLVEESD+ KL YLQG+ISETLRLNP AP+LVPH S +
Sbjct: 835 LNNPDVLKKAREEIDAQIGEKRLVEESDIPKLPYLQGIISETLRLNPTAPMLVPHLTSHD 894
Query: 724 CWIAGYKIPRDTIILINAWAIHRDPNIWEDANTFNPERYKIT--MVD--DLYKLIPFGLG 740
C I+GYKIPRDTI+L+NAWAIHRDPN WE+ F PER++ + +D + KLIPFG+G
Sbjct: 895 CTISGYKIPRDTIVLVNAWAIHRDPNQWEEPMLFKPERHQKSSESIDHHQIPKLIPFGVG 954
BLAST of CmoCh20G009860 vs. NCBI nr
Match:
CAN60309.1 (hypothetical protein VITISV_015004 [Vitis vinifera])
HSP 1 Score: 831.6 bits (2147), Expect = 5.2e-237
Identity = 407/799 (50.94%), Postives = 562/799 (70.34%), Query Frame = 0
Query: 1 MFLDLMYNVIMRMVAGNKPYEH--DKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDP 60
+F +L +N+I RMVAG + Y D ++ FRE++ K + +NPGDF+PIL W+D
Sbjct: 175 LFSELTFNIITRMVAGKRYYGEGSDFEEAKHFREIIRKSFLLSAASNPGDFLPILRWMDY 234
Query: 61 TGLEKKIMKLGRKMDELLQELVDGIRYQKGTG----NTMIDRLLHLQKTDPQNHTDQIIK 120
G EKK+ K R++D +LQ L+D R G NTMID LL LQK++P+ +TDQIIK
Sbjct: 235 GGYEKKMAKNSRELDVILQGLIDEHRSNSKKGLMGNNTMIDHLLSLQKSEPEYYTDQIIK 294
Query: 121 GLIQIILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQECLVNEADLSSLNYL 180
G+ ++ AG DT AVT+EWA+S LLN+P+VL+KAK E+D+ +GQE L+ EADL L+YL
Sbjct: 295 GVTMNLVFAGTDTAAVTMEWAMSLLLNHPDVLKKAKVELDTXVGQERLLEEADLPKLHYL 354
Query: 181 QGIISETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFK 240
Q IISET RL P APL +PH +S + ++GG+D+PRD ++L+N+W +HRDP LW+D T FK
Sbjct: 355 QNIISETFRLCPPAPLWLPHMSSANCQLGGFDIPRDXMLLVNSWTLHRDPKLWDDPTSFK 414
Query: 241 PERHANPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSE 300
PER ++YK LPFG GRRACPG G+A ++ LTLG LIQC+EWER VDM E
Sbjct: 415 PERFEGGERGETYKLLPFGTGRRACPGSGLANKVVGLTLGSLIQCYEWERISEKKVDMME 474
Query: 301 ---------------------------------------------------GHLYLLKRP 360
GHL+LLK P
Sbjct: 475 GKGLTMPKMEPLEAMCSAYEILKXVLQDEETMRNKGKRLNLPPSPPGFPIXGHLHLLKGP 534
Query: 361 VHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYN 420
+H+TL +LS ++G + SLR GS+ V+VVSSPSAV+ECFT ND+I ANRP + K++ Y+
Sbjct: 535 LHRTLHRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFTKNDVIFANRPKFVMGKYIGYD 594
Query: 421 NTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVE 480
T+ S + YGDHWR LRR+S +EIF+++RLN+FL R+DEIK++L +L R S ++FA+VE
Sbjct: 595 YTVVSLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKQLLLRLSRNSVENFAKVE 654
Query: 481 MSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLN 540
+ SM SEL NI MRMV GKRFYG+ + D+E+AR+FR+I K++++ G+ + F P+L
Sbjct: 655 LKSMFSELLLNITMRMVAGKRFYGDNMKDVEEAREFREISKEILEFXGTSNPGDFXPILQ 714
Query: 541 WIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKE---EGRNTMIDHLLSLQQSQLDFMSD 600
WID Y K+ ++LG++ D FLQGL+DE R+ K E RNTMIDHLLSLQ+S+ ++ +D
Sbjct: 715 WIDYQGYNKRALRLGKKMDVFLQGLLDECRSNKRSDLENRNTMIDHLLSLQESEPEYYTD 774
Query: 601 QVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERLVEESDMSK 660
++IKGLI+ + + G DT AVTIEWA+S LLN+P VLKKAR ELD+ IG + L++E+D+ K
Sbjct: 775 EIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETDLPK 834
Query: 661 LSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHRDPNIWEDA 720
L YLQ +ISE+LRL P+ PLLVPH+++E+C + G+ +P T++L+NAWA+HRDP +W D
Sbjct: 835 LQYLQSIISESLRLFPSTPLLVPHFSTEDCKLGGFDVPGGTMLLVNAWALHRDPKLWNDP 894
Query: 721 NTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEWKRVSSEVV 740
+F PER++ T + YKL+PFG+GRRACPG+G+A+RV+GL+L SLIQCF+WKRV + +
Sbjct: 895 TSFKPERFE-TGESETYKLLPFGVGRRACPGIGLANRVMGLTLGSLIQCFDWKRVDEKEI 954
BLAST of CmoCh20G009860 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 494.6 bits (1272), Expect = 1.4e-139
Identity = 244/447 (54.59%), Postives = 338/447 (75.62%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGH--VFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 354
GHL LLK P+H+T LS + +FSLRLG++LV V SS S +ECFT ND++LANRP
Sbjct: 42 GHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRP 101
Query: 355 SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 414
+ + KH+ Y+ T A+SYGDHWR LRRI ++EIFS RLN FL RKDEI+R++ +L
Sbjct: 102 NFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLS 161
Query: 415 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGS 474
R S F +V+M SM+S+L+ N ++RMV GKR+YG+GV D +A++ R ++ V+ G+
Sbjct: 162 RNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGA 221
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
++ ++P+L ++ YE ++ KL R DEFLQGL+DE R KE+G NTMIDHLL+LQ+
Sbjct: 222 GNAVDYLPVLRL--VSDYETRVKKLAGRLDEFLQGLVDEKREAKEKG-NTMIDHLLTLQE 281
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
SQ D+ +D++IKG ++ ++LAGTDT AVT+EWALS++LN+P+VL KAR E+D IG +RL
Sbjct: 282 SQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRL 341
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
++ESD+S L YLQ ++SETLRL PAAP+L+PH ASE+C +AGY +PR TI+L N WAIHR
Sbjct: 342 MDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHR 401
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DP +W+D +F PER++ + KL+PFGLGRRACPG G+AHR++ L+L SLIQC EW
Sbjct: 402 DPQLWDDPMSFKPERFE--KEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEW 461
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
+++ E VDM+EG G+T+P+A+PLEAM
Sbjct: 462 EKIGEE-VDMSEGKGVTMPKAKPLEAM 482
BLAST of CmoCh20G009860 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 488.8 bits (1257), Expect = 7.7e-138
Identity = 241/447 (53.91%), Postives = 327/447 (73.15%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGH--VFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 354
GHL LLK P+H+ +S G + SLRLG++L+ VVSS S +ECFT ND+ILANR
Sbjct: 42 GHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQ 101
Query: 355 SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 414
++ KH++Y N+ ++SY +HWR LRRI +EIFSA RLN F R+DEI+R++G+L
Sbjct: 102 TTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLL 161
Query: 415 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGS 474
R SS F +VEM SM S+L+ N ++RM+ GK +YG+G D +A++ R ++ + M G
Sbjct: 162 RNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGP 221
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
++ +IP+L W IT+ E +I KL R DEFLQGL+DE R KE+ NTM+DHLL LQ+
Sbjct: 222 GNAADYIPILTW--ITYSETRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQE 281
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
+Q ++ D++IKG ++ ++ GTDT AVT+EWALS LLNNP VL KAR E+D +IG +RL
Sbjct: 282 TQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRL 341
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
+EESD+ L YLQ ++SETLRL PAAP+L+PH AS++C + GY +PR T++L NAWAIHR
Sbjct: 342 LEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHR 401
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DP +W+D +F PER++ + KL+PFGLGRRACPG G+A R+V LSL SLIQCFEW
Sbjct: 402 DPLLWDDPTSFKPERFE--KEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEW 461
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
+R+ E VDMTEG GLT+P+A+PLEAM
Sbjct: 462 ERIGEEEVDMTEGPGLTMPKARPLEAM 484
BLAST of CmoCh20G009860 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 480.3 bits (1235), Expect = 2.7e-135
Identity = 240/451 (53.22%), Postives = 331/451 (73.39%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLS-----NKYGHVFSLRLGSQLVVVVSSPS-AVKECFTMNDIIL 354
GHL LLK P+H+TL+ S N G V SLRLGS+LV VVSS A +ECF ND++L
Sbjct: 45 GHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVL 104
Query: 355 ANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVL 414
ANRP I KH+ YNNT A+ YGDHWR LRR+ TIEIFS RLN FL R DE++R++
Sbjct: 105 ANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLI 164
Query: 415 GKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQ 474
+L R++ VE+ M+ +L+ N +MRM+ GKR+YGE D E+A++ R +V V
Sbjct: 165 SRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGA 224
Query: 475 KCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLL 534
S ++ ++P+L + YE ++ KLG+ D+FLQGLID+ R Q+E G TMIDHLL
Sbjct: 225 NTSSGNAVDYVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLL 284
Query: 535 SLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIG 594
LQ+S +++ +DQ+IKG+I+++++AGT+T AVT+EWALS+LLN+P+V+ KAR E+D+ +G
Sbjct: 285 VLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG 344
Query: 595 QERLVEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAW 654
+RL+EE+D+S+L YL+ ++ ETLRL+PA PLLVPH ASE+C I Y +PR T +L+NAW
Sbjct: 345 LDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAW 404
Query: 655 AIHRDPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQ 714
AIHRDPN W+D ++F PER++ ++ KL+ FGLGRRACPG G+A R+VGL+L SLIQ
Sbjct: 405 AIHRDPNTWDDPDSFKPERFE--KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQ 464
Query: 715 CFEWKRVSSEVVDMTEGVGLTIPRAQPLEAM 740
CFEW+RV + VDM EGVG T+P+A PL+A+
Sbjct: 465 CFEWERVGNVEVDMKEGVGNTVPKAIPLKAI 490
BLAST of CmoCh20G009860 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 476.9 bits (1226), Expect = 3.0e-134
Identity = 233/447 (52.13%), Postives = 322/447 (72.04%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGH--VFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 354
GHL+LLK P+H+T LS G+ VF LRLG++LV V+SS S +ECFT ND++LANRP
Sbjct: 43 GHLHLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRP 102
Query: 355 SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 414
I KHL YN T ++SYGDHWR LRRI+ +EIFS RLN FL RKDEI+R++ L
Sbjct: 103 KFTISKHLGYNATYLLSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLS 162
Query: 415 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGS 474
R S F VEM ++++ L+SN +RM+ GKR++GE D + A+ +++V + + G+
Sbjct: 163 RDSLHGFVEVEMKTLLTNLASNTTIRMLAGKRYFGE---DNDDAKLVKNLVSEAVTSAGA 222
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
+ ++ +L W ++ YEK+I LG R D FLQ L+DE R +KE+G TMIDHLL+LQ
Sbjct: 223 GNPIDYLSILRW--VSSYEKRIKNLGNRFDTFLQKLVDEKRAEKEKG-ETMIDHLLALQD 282
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
Q D+ +D +IKG+I+ +++AGTDT +VT+EWA+S+LLN+P +LKKAR+E+D +G +RL
Sbjct: 283 IQPDYYTDVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRL 342
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
V+ESD+ LSYLQ ++ ETLR+ PA PLL+PH +SE+C + GY IP T++L NAWA+HR
Sbjct: 343 VDESDIVNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHR 402
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DP +WED F PER++ + KLI FG+GRRACPG G+AHR++ +L SL+QCFEW
Sbjct: 403 DPEVWEDPEIFKPERFE--KEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEW 462
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
+RV + VDMTE G T+P+A PL AM
Sbjct: 463 ERVGEDFVDMTEDKGATLPKAIPLRAM 481
BLAST of CmoCh20G009860 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 471.5 bits (1212), Expect = 1.3e-132
Identity = 226/447 (50.56%), Postives = 326/447 (72.93%), Query Frame = 0
Query: 295 GHLYLLKRPVHQTLQKLSNKYGH--VFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 354
GHL+LLK+PVH+T +S G+ +F LRLG++LV V+SS S +ECFT ND++LANRP
Sbjct: 43 GHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRP 102
Query: 355 SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 414
+ KH+ YN T A+SYGDHWR LRRI+ +EIFS+ R++ F RKDEI+R++ L
Sbjct: 103 DIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLS 162
Query: 415 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVMQKCGS 474
R S F VE+ S+++ L+ N ++ MV GKR+YG G D ++A+ R+++ ++M GS
Sbjct: 163 RDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGS 222
Query: 475 FDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQGLIDEHRNQKEEGRNTMIDHLLSLQQ 534
+ ++P +NW +T++E + LG R D LQ L+DE R +KE+G+ T+IDHLLS Q+
Sbjct: 223 GNLADYLPSINW--VTNFENQTKILGNRLDRVLQKLVDEKRAEKEKGQ-TLIDHLLSFQE 282
Query: 535 SQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWALSHLLNNPNVLKKARIELDSIIGQERL 594
++ ++ +D +IKG+I+ ++LAGTDT +VT+EWA+S+LLN+P +L+KAR E+D IG +RL
Sbjct: 283 TEPEYYTDVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRL 342
Query: 595 VEESDMSKLSYLQGVISETLRLNPAAPLLVPHYASENCWIAGYKIPRDTIILINAWAIHR 654
VEESD+ L YLQ ++SETLRL PA PLL+PH++S+ C +AGY +PR T++L N WA+HR
Sbjct: 343 VEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHR 402
Query: 655 DPNIWEDANTFNPERYKITMVDDLYKLIPFGLGRRACPGVGMAHRVVGLSLASLIQCFEW 714
DP +WE+ F PER++ + KL+PFG+GRRACPG + R+V L+L LIQ FEW
Sbjct: 403 DPGLWEEPERFKPERFE--KEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEW 462
Query: 715 KRVSSEVVDMTEGVGLTIPRAQPLEAM 740
+RV +E+VDMTEG G+T+P+A PL AM
Sbjct: 463 ERVGAELVDMTEGEGITMPKATPLRAM 484
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 3.6e-140 | 54.38 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ8 | 1.3e-134 | 54.14 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q9FG65 | 3.8e-134 | 53.22 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
P93147 | 6.5e-134 | 51.90 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNQ9 | 3.0e-131 | 52.65 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FUI0 | 1.2e-252 | 100.00 | cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=... | [more] |
A5AJI9 | 2.5e-237 | 50.94 | Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_015004 PE=3 SV=1 | [more] |
A0A7J6G3K7 | 4.3e-237 | 55.16 | Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_006841 PE=3 SV=1 | [more] |
A0A371E3R5 | 3.7e-233 | 50.99 | Cytochrome P450 81E8 (Fragment) OS=Mucuna pruriens OX=157652 GN=CYP81E8 PE=3 SV=... | [more] |
F6HSX8 | 2.1e-231 | 47.66 | Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0129g00860 PE=3 SV=... | [more] |
Match Name | E-value | Identity | Description | |
KAG7034318.1 | 4.2e-255 | 57.22 | Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022944471.1 | 2.6e-252 | 100.00 | cytochrome P450 81D1-like [Cucurbita moschata] | [more] |
XP_023513339.1 | 4.4e-244 | 96.85 | cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo] | [more] |
XP_004141998.3 | 3.8e-240 | 52.52 | uncharacterized protein LOC101215922 [Cucumis sativus] | [more] |
CAN60309.1 | 5.2e-237 | 50.94 | hypothetical protein VITISV_015004 [Vitis vinifera] | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 1.4e-139 | 54.59 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37360.1 | 7.7e-138 | 53.91 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT5G36220.1 | 2.7e-135 | 53.22 | cytochrome p450 81d1 | [more] |
AT4G37330.1 | 3.0e-134 | 52.13 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT4G37320.1 | 1.3e-132 | 50.56 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |